Toshiki Namiki, Minoru Takemoto, Aiko Hayashi, Hiroki Yamagata, Takahiro Ishikawa, Koutaro Yokote, Shu-Yang Li, Masaaki Kubota, Bo-Shi Zhang, Yoichi Yoshida, Tomoo Matsutani, Seiichiro Mine, Toshio Machida, Yoshio Kobayashi, Jiro Terada, Akira Naito, Koichiro Tatsumi, Hirotaka Takizawa, Rika Nakamura, Hideyuki Kuroda, Yasuo Iwadate, Takaki Hiwasa
BACKGROUND: Autoantibodies develop in autoimmune diseases, cancer, diabetes mellitus (DM), and atherosclerosis-related diseases. However, autoantibody biomarkers have not been successfully examined for diagnosis and therapy. METHODS: Serological identification of antigens through recombinant cDNA expression cloning (SEREX) was used for primary screening of antigens. The cDNA product was expressed in bacteria and purified. Amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) was used to evaluate antibody levels in serum samples...
October 30, 2023: BMC Endocrine Disorders