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https://www.readbyqxmd.com/read/28454287/smad3-mutant-mice-develop-colon-cancer-with-overexpression-of-cox-2
#1
Yu-Ping Zhu, Zhuo Liu, Zhi-Xuan Fu, De-Chuan Li
Colon cancer is the second most common cause of cancer-associated mortality in human populations. The aim of the present study was to identify the role of cyclooxygenase-2 (COX-2) in Smad3 mutant mice, which are known to develop colon cancer. Homozygous Smad3 (-/-) mutant mice were generated from inbred and hybrid Smad3 mouse strains by intercrossing the appropriate heterozygotes. Immunohistochemistry with COX-2 antibody was performed throughout this experiment and the data was validated and cross-checked with reverse transcription-polymerase chain reaction (RT-PCR)...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28453996/broca-s-region-a-causal-role-in-implicit-processing-of-grammars-with-crossed-non-adjacent-dependencies
#2
Julia Uddén, Martin Ingvar, Peter Hagoort, Karl Magnus Petersson
Non-adjacent dependencies are challenging for the language learning machinery and are acquired later than adjacent dependencies. In this transcranial magnetic stimulation (TMS) study, we show that participants successfully discriminated between grammatical and non-grammatical sequences after having implicitly acquired an artificial language with crossed non-adjacent dependencies. Subsequent to transcranial magnetic stimulation of Broca's region, discrimination was impaired compared to when a language-irrelevant control region (vertex) was stimulated...
April 25, 2017: Cognition
https://www.readbyqxmd.com/read/28453968/association-of-altered-collagen-content-and-lysyl-oxidase-expression-in-degenerative-mitral-valve-disease
#3
K-Raman Purushothaman, Meerarani Purushothaman, Irene C Turnbull, David H Adams, Anelechi Anyanwu, Prakash Krishnan, Annapoorna Kini, Samin K Sharma, William N O'Connor, Pedro R Moreno
BACKGROUND: Collagen cross-linking is mediated by lysyl oxidase (LOX) enzyme in the extracellular matrix (ECM) of mitral valve leaflets. Alterations in collagen content and LOX protein expression in the ECM of degenerative mitral valve may enhance leaflet expansion and disease severity. METHODS: Twenty posterior degenerative mitral valve leaflets from patients with severe mitral regurgitation were obtained at surgery. Five normal posterior mitral valve leaflets procured during autopsy served as controls...
April 12, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/28453840/modulation-of-the-host-environment-by-human-cytomegalovirus-with-viral-interleukin-10-in-peripheral-blood
#4
Vivian P Young, Margarette C Mariano, Carolyn C Tu, Kathryn M Allaire, Selmir Avdic, Barry Slobedman, Juliet V Spencer
Background.: Human cytomegalovirus (HCMV) is a herpesvirus with both lytic and latent life cycles. Human cytomegalovirus encodes 2 viral cytokines that are orthologs of human cellular interleukin 10 (cIL-10). Both cytomegalovirus interleukin 10 (cmvIL-10) and Latency-associated cytomegalovirus interleukin 10 (LAcmvIL-10) (collectively vIL-10) are expressed during lytic infection and cause immunosuppressive effects that impede virus clearance. LAcmvIL-10 is also expressed during latent infection of myeloid progenitor cells and monocytes and facilitates persistence...
March 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28453587/-association-of-physical-activity-and-dietary-habits-with-overweight-in-fertile-chilean-women
#5
Ximena Guerra M, Paula Bedregal, Gerardo Weisstaub, Patricia Caro M
BACKGROUND: Women in Chile are mainly responsible in the formation of eating habits in the population, particularly at their homes. AIM: To explore the association of physical activity and alimentary habits with overweight among adult Chilean women in fertile age. MATERIAL AND METHODS: Cross sectional study using the data from de National Health Survey 2009-2010. Socio-demographic variables, dietary issues, multidimensional physical activity, sedentary free time and their link with nutritional status were studied in a sample of 1,195 women aged between 20 and 44 years...
February 2017: Revista Médica de Chile
https://www.readbyqxmd.com/read/28453522/potential-biological-pathways-linking-type-d-personality-and-poor-health-a-cross-sectional-investigation
#6
Vera K Jandackova, Julian Koenig, Marc N Jarczok, Joachim E Fischer, Julian F Thayer
BACKGROUND: Type-D personality, defined as a combination of high negative affect and high social isolation, has been associated with poor health outcomes. However, pathways underlying this association are largely unknown. We investigated the relationship between Type-D personality and several biological and behavioral pathways including the autonomic nervous system, the immune system, glucose regulation and sleep in a large, apparently healthy sample. METHODS: Data from a total of 646 respondents (age 41...
2017: PloS One
https://www.readbyqxmd.com/read/28453255/characterization-and-identification-of-dityrosine-cross-linked-peptides-using-tandem-mass-spectrometry
#7
Soumya Mukherjee, Eugene A Kapp, Amber Lothian, Anne M Roberts, Yury Vasilev, Berin Boughton, Kevin Jeffrey Barnham, W Mei Kok, Craig A Hutton, Colin L Masters, Ashley Bush, Joseph S Beckman, Somdatta Ghosh Dey, Blaine R Roberts
The use of mass spectrometry coupled with chemical cross-linking of proteins has become a powerful tool for proteins structure and interactions studies. Unlike structural analysis of proteins using chemical reagents specific for lysine or cysteine residues, identification of gas-phase fragmentation patterns of endogenous dityrosine cross-linked peptides have not been investigated. Dityrosine cross-linking in proteins and peptides are clinical markers of oxidative stress, ageing and neurodegenerative diseases including Alzheimer's disease and Parkinson's disease...
April 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28449085/guanine-quadruplex-monoclonal-antibody-1h6-cross-reacts-with-restrained-thymidine-rich-single-stranded-dna
#8
Hinke G Kazemier, Katrin Paeschke, Peter M Lansdorp
Previously we reported the production and characterization of monoclonal antibody 1H6 raised against (T4G4)2 intermolecular guanine quadruplex (G4) DNA structures (Henderson A. et al. (2014) Nucleic Acids Res., 42, 860-869; Hoffmann R.F. et al. (2016) Nucleic Acids Res., 44, 152-163). It was shown that 1H6 strongly stains nuclei and has exquisite specificity for heterochromatin by immuno-electron microscopy. Here we extend our studies of 1H6 reactivity using enzyme-linked immunosorbent assay (ELISA) and microscale thermophoresis (MST)...
April 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28449070/in-situ-screening-for-postsynaptic-cell-adhesion-molecules-during-synapse-formation
#9
Takeshi Uemura, Tomoko Shiroshima, Asami Maeda, Misato Yasumura, Takashi Shimada, Yuko Fukata, Masaki Fukata, Tomoyuki Yoshida
Neuronal synapse formation is regulated by pre- and postsynaptic cell adhesion molecules. Presynaptic neurexins (NRXNs) and receptor protein tyrosine phosphatases (RPTPs; PTPδ, PTPσ, and LAR in mammals) can induce postsynaptic differentiation through the interaction with various postsynaptic cell adhesion molecules. Here, we developed a novel in situ screening method to identify postsynaptic membranous proteins involved in synaptogenesis. Magnetic beads coated with the extracellular domains of NRXN1β(-S4) and PTPδ-A6 variants preferentially induced excitatory postsynaptic differentiation on the beads' surface when co-cultured with cortical neurons...
April 25, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28448762/bone-turnover-in-osteoporotic-women-during-long-term-oral-bisphosphonates-treatment-implications-for-treatment-failure-and-drug-holiday-in-the-real-world
#10
Yair Liel, Ygal Plakht, Muhammad Abu-Tailakh
OBJECTIVE: Little data exists to support concerns over bone turnover suppression during prolonged oral bisphosphonates treatment and on consequences of the recommended "drug holiday". This study was set to assess bone resorption rates in postmenopausal osteoporotic women on prolonged oral bisphosphonates treatment, and in response to switching to "drug holiday", intravenous bisphosphonate or continuation of oral bisphosphonates. METHODS: Frequency distribution of the bone resorption marker urinary deoxypyridinoline cross-links (uDPD), was obtained retrospectively from 211 osteoporotic women attended at an academic hospital endocrine clinic, treated for >2 years with oral bisphosphonates...
April 27, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/28448614/obtaining-retrotransposon-sequences-analysis-of-their-genomic-distribution-and-use-of-retrotransposon-derived-genetic-markers-in-lentil-lens-culinaris-medik
#11
Rita Rey-Baños, Luis E Sáenz de Miera, Pedro García, Marcelino Pérez de la Vega
Retrotransposons with long terminal repeats (LTR-RTs) are widespread mobile elements in eukaryotic genomes. We obtained a total of 81 partial LTR-RT sequences from lentil corresponding to internal retrotransposon components and LTRs. Sequences were obtained by PCR from genomic DNA. Approximately 37% of the LTR-RT internal sequences presented premature stop codons, pointing out that these elements must be non-autonomous. LTR sequences were obtained using the iPBS technique which amplifies sequences between LTR-RTs...
2017: PloS One
https://www.readbyqxmd.com/read/28448547/circulating-leptin-and-adiponectin-are-associated-with-insulin-resistance-in-healthy-postmenopausal-women-with-hot-flashes
#12
Wan-Yu Huang, Chia-Chu Chang, Dar-Ren Chen, Chew-Teng Kor, Ting-Yu Chen, Hung-Ming Wu
INTRODUCTION: Hot flashes have been postulated to be linked to the development of metabolic disorders. This study aimed to evaluate the relationship between hot flashes, adipocyte-derived hormones, and insulin resistance in healthy, non-obese postmenopausal women. PARTICIPANTS AND DESIGN: In this cross-sectional study, a total of 151 women aged 45-60 years were stratified into one of three groups according to hot-flash status over the past three months: never experienced hot flashes (Group N), mild-to-moderate hot flashes (Group M), and severe hot flashes (Group S)...
2017: PloS One
https://www.readbyqxmd.com/read/28448455/snacking-quality-is-associated-with-secondary-school-academic-achievement-and-the-intention-to-enroll-in-higher-education-a-cross-sectional-study-in-adolescents-from-santiago-chile
#13
Paulina Correa-Burrows, Yanina Rodríguez, Estela Blanco, Sheila Gahagan, Raquel Burrows
Although numerous studies have approached the effects of exposure to a Western diet (WD) on academic outcomes, very few have focused on foods consumed during snack times. We explored whether there is a link between nutritious snacking habits and academic achievement in high school (HS) students from Santiago, Chile. We conducted a cross-sectional study with 678 adolescents. The nutritional quality of snacks consumed by 16-year-old was assessed using a validated food frequency questionnaire. The academic outcomes measured were HS grade point average (GPA), the likelihood of HS completion, and the likelihood of taking college entrance exams...
April 27, 2017: Nutrients
https://www.readbyqxmd.com/read/28448086/effects-of-running-wheel-activity-and-dietary-hmb-and-%C3%AE-alanine-co-supplementation-on-muscle-quality-in-aged-male-rats
#14
D W Russ, C Acksel, K W McCorkle, N K Edens, S M Garvey
OBJECTIVE: Loss of skeletal muscle function is linked to increased risk for loss of health and independence in older adults. Dietary interventions that can enhance aging muscle function, alone or in combination with exercise, may offer an effective way to reduce these risks. The goal of this study was to evaluate the muscular effects of beta-hydroxy-beta-methylbutyrate (HMB) and beta-alanine (β-Ala) co-supplementation in aged Sprague-Dawley rats with voluntary access to running wheels (RW)...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28448077/construct-validation-of-the-dietary-inflammatory-index-among-african-americans
#15
M D Wirth, N Shivappa, L Davis, T G Hurley, A Ortaglia, R Drayton, S N Blair, J R Hébert
OBJECTIVE: Chronic inflammation is linked to many chronic conditions. One of the strongest modulators of chronic inflammation is diet. The Dietary Inflammatory Index (DII) measures dietary inflammatory potential and has been validated previously, but not among African Americans (AAs). DESIGN: Cross-sectional analysis using baseline data from the Healthy Eating and Active Living in the Spirit (HEALS) intervention study. SETTING: Baseline data collection occurred between 2009 and 2012 in or near Columbia, SC...
2017: Journal of Nutrition, Health & Aging
https://www.readbyqxmd.com/read/28448030/synthesis-of-biocompatible-liquid-crystal-elastomer-foams-as-cell-scaffolds-for-3d-spatial-cell-cultures
#16
Marianne E Prévôt, Senay Ustunel, Leah E Bergquist, Richard Cukelj, Yunxiang Gao, Taizo Mori, Lindsay Pauline, Robert J Clements, Elda Hegmann
Here, we present a step-by-step preparation of a 3D, biodegradable, foam-like cell scaffold. These scaffolds were prepared by cross-linking star block co-polymers featuring cholesterol units as side-chain pendant groups, resulting in smectic-A (SmA) liquid crystal elastomers (LCEs). Foam-like scaffolds, prepared using metal templates, feature interconnected microchannels, making them suitable as 3D cell culture scaffolds. The combined properties of the regular structure of the metal foam and of the elastomer result in a 3D cell scaffold that promotes not only higher cell proliferation compared to conventional porous templated films, but also better management of mass transport (i...
April 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447814/undetected-scars-self-criticism-attachment-and-romantic-relationships-among-otherwise-well-functioning-childhood-sexual-abuse-survivors
#17
Dana Lassri, Patrick Luyten, Peter Fonagy, Golan Shahar
BACKGROUND: Studies have consistently demonstrated the negative impact of childhood sexual abuse (CSA) on intimate relationships. The majority of studies have focused on revictimization in at-risk or clinical samples, with very few addressing the impact of CSA on otherwise well-functioning adults and even fewer investigating the psychological mechanisms involved. To fill this gap, this study focuses on the effect of CSA on "normative" (nonviolent) romantic relationships in otherwise well-functioning young women, and the mediating role of personality dimension self-criticism and attachment in this regard...
April 27, 2017: Psychological Trauma: Theory, Research, Practice and Policy
https://www.readbyqxmd.com/read/28447578/proprotein-convertase-subtilisin-kexin-type-9-affects-insulin-but-not-lipid-metabolism-in-cystic-fibrosis
#18
Adèle Coriati, Elizabeth Arslanian, Guillaume F Bouvet, Annik Prat, Nabil G Seidah, Rémi Rabasa-Lhoret, Yves Berthiaume
PURPOSE: Cystic Fibrosis (CF) is the most common genetic disorder and, with improved survival, glucose abnormalities have emerged as a major comorbidity. Proprotein convertase subtilisin/kexin type 9 (PCSK9), a regulator of plasma LDL-cholesterol homeostasis, is associated with lipid and glucose metabolism in healthy individuals. Here we report on the link between PCSK9 and markers of metabolism in CF. METHODS: Cross-sectional analysis was performed on CF patients (≥ 18 years, N=94) from the Montreal Cohort, without known diabetes, and on healthy individuals (N=19)...
April 26, 2017: Clinical and Investigative Medicine. Médecine Clinique et Experimentale
https://www.readbyqxmd.com/read/28447053/deciphering-the-pathophysiology-of-irritable-bowel-syndrome-and-functional-gastrointestinal-disorders-an-alternative-model-for-pathogenesis-cytokine-controlled-transepithelial-multi-feedback-loop
#19
REVIEW
Ricky McCullough, Jeremiah McCullough
A working theoretical model for irritable bowel syndrome (IBS) and other functional gastrointestinal disorders (FGIDs) does not exist, hampered by the lack of any clear cut invention that address all symptom and signs of the disease. Reports of cessation of symptom and signs of both major types of IBS have been published using a non-systemic, topically active agent-high potency polymerized cross-linked sucralfate (HPPCLS). The unique clinical effect of this non-systemic agent restricted to the luminal surface of the gut provides opportunity to elaborate on an alternative working model for the pathogenesis of IBS and FGIDs...
2017: Translational Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28446702/alteration-of-cerebrovascular-haemodynamic-patterns-due-to-atrial-fibrillation-an-in-silico-investigation
#20
S Scarsoglio, A Saglietto, M Anselmino, F Gaita, L Ridolfi
There has recently been growing evidence that atrial fibrillation (AF), the most common cardiac arrhythmia, is independently associated with the risk of dementia. This represents a very recent frontier with high social impact for the number of individuals involved and for the expected increase in AF incidence in the next 40 years. Although a number of potential haemodynamic processes, such as microembolisms, altered cerebral blood flow, hypoperfusion and microbleeds, arise as connecting links between the two pathologies, the causal mechanisms are far from clear...
April 2017: Journal of the Royal Society, Interface
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