Fan Yang, Alireza Labani-Motlagh, Jose Alejandro Bohorquez, Josimar Dornelas Moreira, Danish Ansari, Sahil Patel, Fabrizio Spagnolo, Jon Florence, Abhinav Vankayalapati, Tsuyoshi Sakai, Osamu Sato, Mitsuo Ikebe, Ramakrishna Vankayalapati, John J Dennehy, Buka Samten, Guohua Yi
The continuing emergence of new strains of antibiotic-resistant bacteria has renewed interest in phage therapy; however, there has been limited progress in applying phage therapy to multi-drug resistant Mycobacterium tuberculosis (Mtb) infections. In this study, we show that bacteriophage strains D29 and DS6A can efficiently lyse Mtb H37Rv in 7H10 agar plates. However, only phage DS6A efficiently kills H37Rv in liquid culture and in Mtb-infected human primary macrophages. We further show in subsequent experiments that, after the humanized mice were infected with aerosolized H37Rv, then treated with DS6A intravenously, the DS6A treated mice showed increased body weight and improved pulmonary function relative to control mice...
March 9, 2024: Communications Biology