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https://www.readbyqxmd.com/read/29042750/current-perspective-on-rituximab-in-rheumatic-diseases
#1
REVIEW
Tommaso Schioppo, Francesca Ingegnoli
The steadily increasing knowledge regarding pathogenetic mechanisms in autoimmune rheumatic diseases has paved the way to different therapeutic approaches. In particular, the market entry of biologics has dramatically modified the natural history of rheumatic chronic inflammatory diseases with a meaningful impact on patients' quality of life. Among the wide spectrum of available biological treatments, rituximab (RTX), first used in the treatment of non-Hodgkin's lymphoma, was later approved for rheumatoid arthritis and anti-neutrophil cytoplasmic antibodies-associated vasculitis...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29040878/c-phycocyanin-supplementation-during-in%C3%A2-vitro-maturation-enhances-pre-implantation-developmental-competence-of-parthenogenetic-and-cloned-embryos-in-pigs
#2
Shuang Liang, Jing Guo, Yong Xun Jin, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim
C-Phycocyanin (C-PC), a protein from green microalgae, has been suggested to possess various biological activities, including antioxidant and free radical scavenging properties. The aim of the current study was to explore the effects of C-PC on the maturation of porcine oocytes and subsequent developmental competence after parthenogenetic activation and somatic cell nuclear transfer (SCNT) as well as the underlying mechanisms. There was no significant improvement in nuclear maturation rates between the control and C-PC supplementation groups (1, 3, 5, 10 μg/mL)...
September 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29040674/evaluation-of-apoptotic-and-autophagic-related-protein-expressions-before-and-after-in-vitro-maturation-of-fresh-slow-frozen-and-vitrified-pre-pubertal-mouse-testicular-tissue
#3
L Dumont, F Chalmel, A Oblette, B Berby, A Rives, V Duchesne, C Rondanino, N Rives
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: In vitro maturation strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i...
October 11, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29036896/remote-sensing-based-quantification-of-the-impact-of-flash-flooding-on-the-rice-production-a-case-study-over-northeastern-bangladesh
#4
M Razu Ahmed, Khan Rubayet Rahaman, Aaron Kok, Quazi K Hassan
The northeastern region of Bangladesh often experiences flash flooding during the pre-harvesting period of the boro rice crop, which is the major cereal crop in the country. In this study, our objective was to delineate the impact of the 2017 flash flood (that initiated on 27 March 2017) on boro rice using multi-temporal Landsat-8 OLI and MODIS data. Initially, we opted to use Landsat-8 OLI data for mapping the damages; however, during and after the flooding event the acquisition of cloud free images were challenging...
October 14, 2017: Sensors
https://www.readbyqxmd.com/read/29033393/new-bioactive-glass-scaffolds-with-exceptional-qualities-for-bone-tissue-regeneration-response-of-osteoblasts-and-osteoclasts
#5
Tia Kowal, Natalie C Hahn, Stephanie Eider, Jutta Marzillier, Daniella Marie Fodera, Ukrit Thamma, Himanshu Jain, Matthias M Falk
Tissue regeneration is a significantly improved alternative to tissue replacement by implants. It requires porous bioscaffolds for the restoration of natural tissue rather than relying on bio-inactive, often metallic implants. Recently, we developed technology for fabricating novel, nano-macroporous bioactive 'Tailored Amorphous Multi-Porous (TAMP)' hard tissue scaffolds using a 70 mol% SiO2-30 mol% CaO model composition. The TAMP silicate scaffolds, fabricated by a modified sol-gel process, have shown excellent biocompatibility via the rapid formation of hydroxyapatite in biological fluids as well as in early tests with bone forming cells...
October 16, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/29032448/effect-of-single-residue-bulges-on-rna-double-helical-structures-crystallographic-database-analysis-and-molecular-dynamics-simulation-studies
#6
Angana Ray, Ankita Agarwal, Dhananjay Bhattacharyya
Asymmetric bulge loop motifs are widely dispersed in all types of functional RNAs. They are frequently occurring structural motifs in folded RNA structures and appear commonly in pre-microRNA and ribosomes, where they are involved in specific RNA-RNA and RNA-protein interactions. It is therefore necessary to understand such motifs from a structural point of view. We analyzed all available RNA structures and identified quite a few fragments of double helices that contain bulges. We found that these discontinuities often introduce kinks into the double helices, which also affects the stacking overlap between the base pairs across the irregularity...
October 14, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29032146/microrna-single-polynucleotide-polymorphism-influences-on-microrna-biogenesis-and-mrna-target-specificity
#7
REVIEW
Jarosław Króliczewski, Aleksandra Sobolewska, Dawid Lejnowski, James F Collawn, Rafał Bartoszewski
microRNAs (miRNAs) are nowadays recognized as an essential component of gene regulatory networks. Furthermore, deregulation of miRNAs expression often contributes to human pathologies. Recently, a substantial number of single nucleotide polymorphism (SNPs) and rare mutations within pri-, pre- and mature miRNA sequences have been reported. These miRNA SNPs have often been associated with human disease. However, due to the complexity of miRNA biogenesis and the genome-wide functional effects of miRNAs, the determination of biological consequences of these miRNA SNPs remains challenging...
October 12, 2017: Gene
https://www.readbyqxmd.com/read/29031730/bidirectional-manipulation-of-gene-expression-in-adipocytes-using-crispra-and-sirna
#8
Morten Lundh, Kaja Pluciñska, Marie S Isidor, Patricia S S Petersen, Brice Emanuelli
OBJECTIVE: Functional investigation of novel gene/protein targets associated with adipocyte differentiation or function heavily relies on efficient and accessible tools to manipulate gene expression in adipocytes in vitro. Recent advances in gene-editing technologies such as CRISPR-Cas9 have not only eased gene editing but also greatly facilitated modulation of gene expression without altering the genome. Here, we aimed to develop and validate a competent in vitro adipocyte model of controllable functionality as well as multiplexed gene manipulation in adipocytes, using the CRISPRa "SAM" system and siRNAs to simultaneously overexpress and silence selected genes in the same cell populations...
October 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/29030553/actomyosin-polarisation-through-plc-pkc-triggers-symmetry-breaking-of-the-mouse-embryo
#9
Meng Zhu, Chuen Yan Leung, Marta N Shahbazi, Magdalena Zernicka-Goetz
Establishment of cell polarity in the mammalian embryo is fundamental for the first cell fate decision that sets aside progenitor cells for both the new organism and the placenta. Yet the sequence of events and molecular mechanism that trigger this process remain unknown. Here, we show that de novo polarisation of the mouse embryo occurs in two distinct phases at the 8-cell stage. In the first phase, an apical actomyosin network is formed. This is a pre-requisite for the second phase, in which the Par complex localises to the apical domain, excluding actomyosin and forming a mature apical cap...
October 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29029549/progress-and-future-prospect-of-in-vitro-spermatogenesis
#10
REVIEW
Fahar Ibtisham, Jiang Wu, Mei Xiao, Lilong An, Zachary Banker, Aamir Nawab, Yi Zhao, Guanghui Li
Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40-50% is due to "male factor" infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment. Developments in reproductive biotechnology have made it possible to rescue fertility and uphold biological fatherhood. In vitro production of haploid male germ cell is a powerful tool, not only for the treatment of infertility including oligozoospermic or azoospermic patient, but also for the fertility preservation in pre-pubertal boys whose gonadal function is threatened by gonadotoxic therapies...
September 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29026709/needle-aspiration-biopsy-of-seminiferous-tubules-nabst-as-a-diagnostic-method-in-korea
#11
Jay Ho Jung, Tae Ho Lee, Kyung Hwa Choi, Jae Yup Hong
To evaluate the diagnostic efficacy of needle aspiration biopsy of seminiferous tubules (NABST) and to represent the redistributed diagnostic results corresponding to testicular volumes and follicle-stimulating hormone (FSH) levels. In this retrospective study, we investigated 65 infertile men with either azoospermia or oligoasthenoteratozoospermia. Following NABST, specimens were stained with hematoxylin and eosin and classified into five histological types. With pre-procedure FSH levels and testicular volumes, we evaluated the probabilities of detecting sperms within biopsy specimens...
September 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/29026527/recent-advances-in-in-vitro-fertilization
#12
REVIEW
Robert Casper, Jigal Haas, Tzu-Bou Hsieh, Rawad Bassil, Chaula Mehta
The field of assisted reproductive technology is rapidly progressing with many new advances in the last decade. The present review discusses methods to improve oocyte quality in older women and new stimulation protocols that may improve the number of mature oocytes retrieved during an in vitro fertilization cycle. We will discuss the present use of pre-implantation genetic screening (PGS) and finally focus on some new methods to determine endometrial receptivity. The focus of this review is to point out areas of technology that may be controversial or are new enough to require proper controlled studies for validation...
2017: F1000Research
https://www.readbyqxmd.com/read/29026134/mct1-and-mct4-expression-and-lactate-flux-activity-increase-during-white-and-brown-adipogenesis-and-impact-adipocyte-metabolism
#13
Charlotte Petersen, Mette D Nielsen, Elise S Andersen, Astrid L Basse, Marie S Isidor, Lasse K Markussen, Birgitte M Viuff, Ian H Lambert, Jacob B Hansen, Stine F Pedersen
Adipose tissue takes up glucose and releases lactate, thereby contributing significantly to systemic glucose and lactate homeostasis. This implies the necessity of upregulation of net acid and lactate flux capacity during adipocyte differentiation and function. However, the regulation of lactate- and acid/base transporters in adipocytes is poorly understood. Here, we tested the hypothesis that adipocyte thermogenesis, browning and differentiation are associated with an upregulation of plasma membrane lactate and acid/base transport capacity that in turn is important for adipocyte metabolism...
October 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29024760/rna-processing-in-the-male-germline-mechanisms-and-implications-for-fertility
#14
REVIEW
Julien M D Legrand, Robin M Hobbs
Mammalian spermatogenesis is a tightly coordinated process that gives rise to mature spermatozoa capable of fertilising an ovum during sexual reproduction. A population of stem and progenitor cells known as undifferentiated spermatogonia enables continual spermatogenesis throughout life. A complex transcriptional network that balances self-renewal of spermatogonia with their timely differentiation in order to maintain constant fertility regulates this process. Importantly, post-transcriptional regulation of gene expression plays a critical role in spermatogenesis, necessitated by the profound genetic and morphological changes that occur during meiosis and sperm maturation...
October 9, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29023331/effects-of-plyometric-training-on-components-of-physical-fitness-in-prepuberal-male-soccer-athletes-the-role-of-surface-instability
#15
Yassine Negra, Helmi Chaabene, Senda Sammoud, Raja Bouguezzi, Bessem Mkaouer, Younés Hachana, Urs Granacher
Negra, Y, Chaabene, H, Sammoud, S, Bouguezzi, R, Mkaouer, B, Hachana, Y, and Granacher, U. Effects of plyometric training on components of physical fitness in prepuberal male soccer athletes: the role of surface instability. J Strength Cond Res XX(X): 000-000, 2017-Previous studies contrasted the effects of plyometric training (PT) conducted on stable vs. unstable surfaces on components of physical fitness in child and adolescent soccer players. Depending on the training modality (stable vs. unstable), specific performance improvements were found for jump (stable PT) and balance performances (unstable PT)...
October 10, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29021243/zucchini-dependent-pirna-processing-is-triggered-by-recruitment-to-the-cytoplasmic-processing-machinery
#16
Alicia K Rogers, Kathy Situ, Edward M Perkins, Katalin Fejes Toth
The piRNA pathway represses transposable elements in the gonads and thereby plays a vital role in protecting the integrity of germline genomes of animals. Mature piRNAs are processed from longer transcripts, piRNA precursors (pre-piRNAs). In Drosophila, processing of pre-piRNAs is initiated by piRNA-guided Slicer cleavage or the endonuclease Zucchini (Zuc). As Zuc does not have any sequence or structure preferences in vitro, it is not known how piRNA precursors are selected and channeled into the Zuc-dependent processing pathway...
October 11, 2017: Genes & Development
https://www.readbyqxmd.com/read/29020459/effect-of-pre-harvest-and-post-harvest-conditions-on-the-fruit-allergenicity-a-review
#17
Jin Wang, Sai Kranthi Vanga, Vijaya Raghavan
Fruits are an important source of vitamins and antioxidants that can effectively delay aging and contribute to health and well-being of the human kind. However, they are growing to be one of the primary elicitors of food allergies around the world. Fruit allergens can induce an IgE-mediated (Immunoglobulin E) reaction, presenting with a symptom like localized oral allergy syndrome (OAS). Numerous studies showed that varying environmental and cultivation conditions can influence the fruit allergen content during flowering and ripening stages...
October 11, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29017919/whole-cell-biopanning-with-a-synthetic-phage-display-library-of-nanobodies-enabled-the-recovery-of-follicle-stimulating-hormone-receptor-inhibitors
#18
Ronan Crepin, Gianluca Veggiani, Selma Djender, Anne Beugnet, François Planeix, Christophe Pichon, Sandrine Moutel, Sebastian Amigorena, Franck Perez, Nicolae Ghinea, Ario de Marco
Antibodies are essential reagents that are increasingly used in diagnostics and therapy. Their specificity and capacity to recognize their native antigen are critical characteristics for their in vivo application. Follicle-stimulating hormone receptor is a GPCR protein regulating ovarian follicular maturation and spermatogenesis. Recently, its potentiality as a cancer biomarker has been demonstrated but no antibody suitable for in vivo tumor targeting and treatment has been characterized so far. In this paper we describe the first successful attempt to recover recombinant antibodies against the FSHR and that: i) are directly panned from a pre-immune library using whole cells expressing the target receptor at their surface; ii) show inhibitory activity towards the FSH-induced cAMP accumulation; iii) do not share the same epitope with the natural binder FSH; iv) can be produced inexpensively as mono- or bivalent functional molecules in the bacterial cytoplasm...
October 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29016763/a-cephalometric-study-of-the-skeletal-and-dento-alveolar-effects-of-the-modified-louisiana-state-university-activator-in-class-ii-malocclusion
#19
Yunlong Kang, Lorenzo Franchi, David J Manton, Paul M Schneider
Objectives: To analyse the skeletal and dento-alveolar effects of the modified Louisiana State University activator (MLSUA) in Class II treatment. Materials and methods: A test group of 46 participants (25 females, 21 males) with Class II malocclusion treated with MLSUA followed by fixed appliances was compared with a matched Class II control group. Lateral cephalograms were taken at T1 (initial records), T2 (completion of MLSUA treatment), and T3 (before deband)...
July 11, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28994389/molecular-architecture-underlying-fluid-absorption-by-the-developing-inner-ear
#20
Keiji Honda, Sung Huhn Kim, Michael C Kelly, Joseph C Burns, Laura Constance, Xiangming Li, Fei Zhou, Michael Hoa, Matthew W Kelley, Philine Wangemann, Robert J Morell, Andrew J Griffith
Mutations of SLC26A4 are a common cause of hearing loss associated with enlargement of the endolymphatic sac (EES). Slc26a4 expression in the developing mouse endolymphatic sac is required for acquisition of normal inner ear structure and function. Here, we show that the mouse endolymphatic sac absorbs fluid in an SLC26A4-dependent fashion. Fluid absorption was sensitive to ouabain and gadolinium but insensitive to benzamil, bafilomycin and S3226. Single-cell RNA-seq analysis of pre- and postnatal endolymphatic sacs demonstrates two types of differentiated cells...
October 10, 2017: ELife
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