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https://www.readbyqxmd.com/read/27843019/the-trypanosoma-cruzi-surface-a-nanoscale-patchwork-quilt
#1
REVIEW
Juan Mucci, Andrés B Lantos, Carlos A Buscaglia, María Susana Leguizamón, Oscar Campetella
The Trypanosoma cruzi trypomastigote membrane provides a major protective role against mammalian host-derived defense mechanisms while allowing the parasite to interact with different cell types and trigger pathogenesis. This surface has been historically appreciated as a rather unstructured 'coat', mainly consisting of a continuous layer of glycolipids and heavily O-glycosylated mucins, occasionally intercalated with different developmentally regulated molecules displaying adhesive and/or enzymatic properties...
November 11, 2016: Trends in Parasitology
https://www.readbyqxmd.com/read/27714511/interventional-eus-using-a-flexible-19-gauge-needle-an-international-multicenter-experience-in-162-patients
#2
Vivek Kumbhari, Irene Peñas, Alan H Tieu, Carlos De la Serna-Higuera, Manie Juneja, Fuad Maufa, Saowanee Ngamruengphong, Mohamad H El-Zein, Nadim Haddad, Sandeep Krishnan, Susana Gonzalez, Peter V Renny, Payal Saxena, Linda Howard, Christopher J DiMaio, Jonathan M Buscaglia, Manuel Perez-Miranda, Mouen A Khashab
BACKGROUND AND AIMS: To facilitate access for interventional EUS, flexible (nitinol) 19-G needles have been introduced to permit needle puncture even when the echoendoscope is in an angulated position, such as in the second part of the duodenum. The aims of the study were to evaluate the performance of a flexible 19-G needle during interventional EUS procedures and compare outcomes when the echoendoscope was in the straight versus angulated position. METHODS: A retrospective review was undertaken of 162 consecutive patients that underwent a variety of interventional EUS procedures with a flexible 19-G needle across five centers...
December 2016: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/27693206/randomized-evidence-for-reduction-of-perioperative-mortality-an-updated-consensus-process
#3
Giovanni Landoni, Antonio Pisano, Vladimir Lomivorotov, Gabriele Alvaro, Ludhmila Hajjar, Gianluca Paternoster, Caetano Nigro Neto, Nicola Latronico, Evgeny Fominskiy, Laura Pasin, Gabriele Finco, Rosetta Lobreglio, Maria Luisa Azzolini, Giuseppe Buscaglia, Alberto Castella, Marco Comis, Adele Conte, Massimiliano Conte, Francesco Corradi, Erika Dal Checco, Giovanni De Vuono, Marco Ganzaroli, Eugenio Garofalo, Gordana Gazivoda, Rosalba Lembo, Daniele Marianello, Martina Baiardo Redaelli, Fabrizio Monaco, Valentina Tarzia, Marta Mucchetti, Alessandro Belletti, Paolo Mura, Mario Musu, Giovanni Pala, Massimiliano Paltenghi, Vadim Pasyuga, Desiderio Piras, Claudio Riefolo, Agostino Roasio, Laura Ruggeri, Francesco Santini, Andrea Székely, Luigi Verniero, Antonella Vezzani, Alberto Zangrillo, Rinaldo Bellomo
OBJECTIVE: Of the 230 million patients undergoing major surgical procedures every year, more than 1 million will die within 30 days. Thus, any nonsurgical interventions that help reduce perioperative mortality might save thousands of lives. The authors have updated a previous consensus process to identify all the nonsurgical interventions, supported by randomized evidence, that may help reduce perioperative mortality. DESIGN AND SETTING: A web-based international consensus conference...
August 2, 2016: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/27639210/microscopical-characterization-of-known-postmortem-root-bands-using-light-and-scanning-electron-microscopy
#4
Jack Hietpas, JoAnn Buscaglia, Adam H Richard, Stephen Shaw, Hilda S Castillo, Joseph Donfack
A postmortem root band (PMRB) is a distinct microscopic feature that is postulated to occur in hair remaining in the follicle during the postmortem interval [1] (Petraco et al., 1998). The scientific validity of this premise has been highlighted in two recent high-profile criminal cases involving PMRBs [2,3] (State of Florida v. Casey Marie Anthony, 2008; People v. Kogut, 2005). To better understand the fundamental aspects of postmortem root banding, the microscopical properties of known PMRBs(1) were characterized by light microscopy, and scanning electron microscope (SEM) imaging of microtomed sections of hairs showing root banding...
October 2016: Forensic Science International
https://www.readbyqxmd.com/read/27629703/trim9-mediates-netrin-1-induced-neuronal-morphogenesis-in-the-developing-and-adult-hippocampus
#5
Jayne Aiken, Georgia Buscaglia
No abstract text is available yet for this article.
September 14, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27520399/issue-highlights
#6
Jonathan M Buscaglia
No abstract text is available yet for this article.
October 2016: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27477511/nrf2-promotes-tumor-maintenance-by-modulating-mrna-translation-in-pancreatic-cancer
#7
Iok In Christine Chio, Seyed Mehdi Jafarnejad, Mariano Ponz-Sarvise, Youngkyu Park, Keith Rivera, Wilhelm Palm, John Wilson, Vineet Sangar, Yuan Hao, Daniel Öhlund, Kevin Wright, Dea Filippini, Eun Jung Lee, Brandon Da Silva, Christina Schoepfer, John Erby Wilkinson, Jonathan M Buscaglia, Gina M DeNicola, Herve Tiriac, Molly Hammell, Howard C Crawford, Edward E Schmidt, Craig B Thompson, Darryl J Pappin, Nahum Sonenberg, David A Tuveson
Pancreatic cancer is a deadly malignancy that lacks effective therapeutics. We previously reported that oncogenic Kras induced the redox master regulator Nfe2l2/Nrf2 to stimulate pancreatic and lung cancer initiation. Here, we show that NRF2 is necessary to maintain pancreatic cancer proliferation by regulating mRNA translation. Specifically, loss of NRF2 led to defects in autocrine epidermal growth factor receptor (EGFR) signaling and oxidation of specific translational regulatory proteins, resulting in impaired cap-dependent and cap-independent mRNA translation in pancreatic cancer cells...
August 11, 2016: Cell
https://www.readbyqxmd.com/read/27349928/impact-and-outcomes-of-research-sponsored-by-the-american-society-for-gastrointestinal-endoscopy
#8
Mohammad F Madhoun, Gregory A Cote, Sushil K Ahlawat, Nuzhat A Ahmad, Jonathan M Buscaglia, Audrey H Calderwood, Seth Crockett, Dayna S Early, Ferga C Gleeson, Suryakanth R Gurudu, Thomas F Imperiale, Julia J Liu, Patrick Mosler, Rahul Pannala, Patrick R Pfau, Joseph Romagnuolo, Jewel Samadder, Amrita Sethi, Amandeep K Shergill, Eun J Shin, Field Willingham, Jason A Dominitz
BACKGROUND AND AIMS: Since 1985, the American Society for Gastrointestinal Endoscopy (ASGE) has awarded grants for endoscopic-related research. The goals of this study were to examine trends in ASGE grant funding and to assess productivity of previous recipients of the ASGE grant awards. METHODS: This was a retrospective cohort analysis of all research grants awarded by the ASGE through 2009. Measures of academic productivity and self-assessment of the ASGE awards' impact on the recipients' careers were defined by using publicly available resources (eg, National Library of Medicine-PubMed) and administration of an electronic survey to award recipients...
September 2016: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/27297851/a-randomized-controlled-trial-of-levosimendan-to-reduce-mortality-in-high-risk-cardiac-surgery-patients-cheetah-rationale-and-design
#9
Alberto Zangrillo, Gabriele Alvaro, Antonio Pisano, Fabio Guarracino, Rosetta Lobreglio, Nikola Bradic, Rosalba Lembo, Stefano Gianni, Maria Grazia Calabrò, Valery Likhvantsev, Evgeny Grigoryev, Giuseppe Buscaglia, Giovanni Pala, Elisabetta Auci, Bruno Amantea, Fabrizio Monaco, Giovanni De Vuono, Antonio Corcione, Nicola Galdieri, Claudia Cariello, Tiziana Bove, Evgeny Fominskiy, Stefano Auriemma, Massimo Baiocchi, Alessandro Bianchi, Mario Frontini, Gianluca Paternoster, Fabio Sangalli, Chew-Yin Wang, Maria Chiara Zucchetti, Giuseppe Biondi-Zoccai, Marco Gemma, Michael J Lipinski, Vladimir V Lomivorotov, Giovanni Landoni
OBJECTIVE: Patients undergoing cardiac surgery are at risk of perioperative low cardiac output syndrome due to postoperative myocardial dysfunction. Myocardial dysfunction in patients undergoing cardiac surgery is a potential indication for the use of levosimendan, a calcium sensitizer with 3 beneficial cardiovascular effects (inotropic, vasodilatory, and anti-inflammatory), which appears effective in improving clinically relevant outcomes. DESIGN: Double-blind, placebo-controlled, multicenter randomized trial...
July 2016: American Heart Journal
https://www.readbyqxmd.com/read/27294185/data-on-the-interexaminer-variation-of-minutia-markup-on-latent-fingerprints
#10
Bradford T Ulery, R Austin Hicklin, Maria Antonia Roberts, JoAnn Buscaglia
The data in this article supports the research paper entitled "Interexaminer variation of minutia markup on latent fingerprints" [1]. The data in this article describes the variability in minutia markup during both analysis of the latents and comparison between latents and exemplars. The data was collected in the "White Box Latent Print Examiner Study," in which each of 170 volunteer latent print examiners provided detailed markup documenting their examinations of latent-exemplar pairs of prints randomly assigned from a pool of 320 pairs...
September 2016: Data in Brief
https://www.readbyqxmd.com/read/27193921/quantitative-real-time-detection-of-carcinoembryonic-antigen-cea-from-pancreatic-cyst-fluid-using-3-d-surface-molecular-imprinting
#11
Yingjie Yu, Qi Zhang, Jonathan Buscaglia, Chung-Chueh Chang, Ying Liu, Zhenhua Yang, Yichen Guo, Yantian Wang, Kalle Levon, Miriam Rafailovich
In this study, a sensitive, yet robust, biosensing system with real-time electrochemical readout was developed. The biosensor system was applied to the detection of carcinoembryonic antigen (CEA), which is a common marker for many cancers such as pancreatic, breast, and colon cancer. Real time detection of CEA during a medical procedure can be used to make critical decisions regarding further surgical intervention. CEA was templated on gold surface (RMS roughness ∼3-4 nm) coated with a hydrophilic self-assembled monolayer (SAM) on the working electrode of an open circuit potentiometric network...
July 21, 2016: Analyst
https://www.readbyqxmd.com/read/27122358/label-free-detection-of-dna-single-base-mismatches-using-a-simple-reflectance-based-optical-technique
#12
G Nava, E Ceccarello, F Giavazzi, M Salina, F Damin, M Chiari, M Buscaglia, T Bellini, G Zanchetta
Rapid and quantitative detection of the binding of nucleic acids to surface-immobilized probes remains a challenge in many biomedical applications. We investigated the hybridization of a set of fully complementary and defected 12-base long DNA oligomers by using the Reflective Phantom Interface (RPI), a recently developed multiplexed label-free detection technique. Based on the simple measurement of reflected light intensity, this technology enables to quantify the hybridization directly as it occurs on the surface with a sensitivity of 10 pg mm(-2)...
May 21, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27058585/sialic-acid-glycobiology-unveils-trypanosoma-cruzi-trypomastigote-membrane-physiology
#13
Andrés B Lantos, Giannina Carlevaro, Beatriz Araoz, Pablo Ruiz Diaz, María de Los Milagros Camara, Carlos A Buscaglia, Mariano Bossi, Hai Yu, Xi Chen, Carolyn R Bertozzi, Juan Mucci, Oscar Campetella
Trypanosoma cruzi, the flagellate protozoan agent of Chagas disease or American trypanosomiasis, is unable to synthesize sialic acids de novo. Mucins and trans-sialidase (TS) are substrate and enzyme, respectively, of the glycobiological system that scavenges sialic acid from the host in a crucial interplay for T. cruzi life cycle. The acquisition of the sialyl residue allows the parasite to avoid lysis by serum factors and to interact with the host cell. A major drawback to studying the sialylation kinetics and turnover of the trypomastigote glycoconjugates is the difficulty to identify and follow the recently acquired sialyl residues...
April 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27046517/interexaminer-variation-of-minutia-markup-on-latent-fingerprints
#14
Bradford T Ulery, R Austin Hicklin, Maria Antonia Roberts, JoAnn Buscaglia
Latent print examiners often differ in the number of minutiae they mark during analysis of a latent, and also during comparison of a latent with an exemplar. Differences in minutia counts understate interexaminer variability: examiners' markups may have similar minutia counts but differ greatly in which specific minutiae were marked. We assessed variability in minutia markup among 170 volunteer latent print examiners. Each provided detailed markup documenting their examinations of 22 latent-exemplar pairs of prints randomly assigned from a pool of 320 pairs...
July 2016: Forensic Science International
https://www.readbyqxmd.com/read/27038277/laparoscopic-right-colectomy-versus-laparoscopic-assisted-colonoscopic-polypectomy-for-endoscopically-unresectable-polyps-a-randomised-controlled-trial
#15
Chris Lascarides, Jonathan M Buscaglia, Paula I Denoya, Satish Nagula, Juan Carlos Bucobo, Roberto Bergamaschi
AIM: An RCT was conducted to test the null hypothesis that there is no difference in complication rates and length of stay (LOS) between laparoscopic right colectomy (LRC) and laparoscopic-assisted colonoscopic polypectomy (LACP) for endoscopically unresectable polyps of the right colon. METHOD: A single-centre RCT (NCT01986699) was conducted on patients with polyps of the right colon deemed non-resectable by the gastroenterologist. They underwent a repeat colonoscopy with biopsy by an interventional endoscopist and were allocated to LRC or LACP...
April 1, 2016: Colorectal Disease: the Official Journal of the Association of Coloproctology of Great Britain and Ireland
https://www.readbyqxmd.com/read/26953946/relationship-between-values-of-fowlpox-elisa-and-the-presence-of-takes-after-vaccination
#16
Celina Buscaglia Barreda
Values from an ELISA for evaluating the immune response induced by a commercial vaccine against fowlpox virus and the lesion at the site of inoculation (i.e., swelling of the skin or a pox where the vaccine was applied) were compared. The ELISA was carried out with an antigen prepared by precipitation of a cell culture-propagated virus suspension with ammonium sulfate and concentration by centrifugation. A 0.1 M acetate buffer (pH 5) was used as the sensitizing solution for maximum specific binding of the antigen to the microplate plastic well...
March 2016: Avian Diseases
https://www.readbyqxmd.com/read/26874684/function-and-regulation-of-trpm7-as-well-as-intracellular-magnesium-content-are-altered-in-cells-expressing-%C3%AE-f508-cftr-and-g551d-cftr
#17
F Huguet, M L Calvez, N Benz, S Le Hir, O Mignen, P Buscaglia, F D Horgen, C Férec, M Kerbiriou, P Trouvé
Cystic fibrosis (CF), one of the most common fatal hereditary disorders, is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. The CFTR gene product is a multidomain adenosine triphosphate-binding cassette (ABC) protein that functions as a chloride (Cl(-)) channel that is regulated by intracellular magnesium [Mg(2+)]i. The most common mutations in CFTR are a deletion of a phenylalanine residue at position 508 (ΔF508-CFTR, 70-80 % of CF phenotypes) and a Gly551Asp substitution (G551D-CFTR, 4-5 % of alleles), which lead to decreased or almost abolished Cl(-) channel function, respectively...
September 2016: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/26802749/electrochemical-magnetic-microbeads-based-biosensor-for-point-of-care-serodiagnosis-of-infectious-diseases
#18
María E Cortina, Luciano J Melli, Mariano Roberti, Mijal Mass, Gloria Longinotti, Salvador Tropea, Paulina Lloret, Diego A Rey Serantes, Francisco Salomón, Matías Lloret, Ana J Caillava, Sabrina Restuccia, Jaime Altcheh, Carlos A Buscaglia, Laura Malatto, Juan E Ugalde, Liliana Fraigi, Carlos Moina, Gabriel Ybarra, Andrés E Ciocchini, Diego J Comerci
Access to appropriate diagnostic tools is an essential component in the evaluation and improvement of global health. Additionally, timely detection of infectious agents is critical in early diagnosis and treatment of infectious diseases. Conventional pathogen detection methods such as culturing, enzyme linked immunosorbent assay (ELISA) or polymerase chain reaction (PCR) require long assay times, and complex and expensive instruments making them not adaptable to point-of-care (PoC) needs at resource-constrained places and primary care settings...
June 15, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/26796073/thermoelectric-behavior-of-ruddlesden-popper-series-iridates
#19
I Pallecchi, M T Buscaglia, V Buscaglia, E Gilioli, G Lamura, F Telesio, M R Cimberle, D Marré
The goal of this work is to study the evolution of thermoelectric transport across the members of the Ruddlesden-Popper series iridates [Formula: see text], where a metal-insulator transition driven by bandwidth change occurs, from the strongly insulating Sr2IrO4 to the metallic non Fermi liquid behavior of SrIrO3. Sr2IrO4 ([Formula: see text]), Sr3Ir2O7 ([Formula: see text]) and SrIrO3 ([Formula: see text]) polycrystals are synthesized at high pressure and characterized by structural, magnetic, electric and thermoelectric transport analyses...
February 17, 2016: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/26713890/get-ready-to-meet-the-growing-demand-from-patients-with-pancreatic-cysts
#20
EDITORIAL
Mohamed O Othman, Jonathan M Buscaglia
No abstract text is available yet for this article.
April 2016: Clinical Gastroenterology and Hepatology
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