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https://www.readbyqxmd.com/read/29774024/quantification-of-hla-dm-dependent-major-histocompatibility-complex-of-class-ii-immunopeptidomes-by-the-peptide-landscape-antigenic-epitope-alignment-utility
#1
Miguel Álvaro-Benito, Eliot Morrison, Esam T Abualrous, Benno Kuropka, Christian Freund
The major histocompatibility complex of class II (MHCII) immunopeptidome represents the repertoire of antigenic peptides with the potential to activate CD4+ T cells. An understanding of how the relative abundance of specific antigenic epitopes affects the outcome of T cell responses is an important aspect of adaptive immunity and offers a venue to more rationally tailor T cell activation in the context of disease. Recent advances in mass spectrometric instrumentation, computational power, labeling strategies, and software analysis have enabled an increasing number of stratified studies on HLA ligandomes, in the context of both basic and translational research...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29765984/jazf1-inhibits-adipose-tissue-macrophages-and-adipose-tissue-inflammation-in-diet-induced-diabetic-mice
#2
Fanping Meng, Yao Lin, Min Yang, Minyan Li, Gangyi Yang, Po Hao, Ling Li
Background: Juxtaposed with another zinc finger gene 1 (JAZF1) affects gluconeogenesis, insulin sensitivity, lipid metabolism, and inflammation, but its exact role in chronic inflammation remains unclear. This study aimed to examine JAZF1 overexpression in vivo on adipose tissue macrophages (ATMs). Methods: Mouse models of high-fat diet- (HFD-) induced insulin resistance were induced using C57BL/6J and JAZF1-overexpressing (JAZF1-OX) mice. The mice were randomized (8-10/group) to C57BL/6J mice fed regular diet (RD) (NC group), C57BL/6J mice fed HFD (HF group), JAZF1-OX mice fed RD (NJ group), and JAZF1-OX mice fed HFD (HJ group)...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29764680/the-parasite-derived-rov-asp-1-is-an-effective-antigen-sparing-cd4-t-cell-dependent-adjuvant-for-the-trivalent-inactivated-influenza-vaccine-and-functions-in-the-absence-of-myd88-pathway
#3
Sonia Jain, Parakkal Jovvian George, Wanyan Deng, Joseph Koussa, Kaela Parkhouse, Scott E Hensley, Jiu Jiang, Jie Lu, Zhuyun Liu, Junfei Wei, Bin Zhan, Maria Elena Bottazzi, Hao Shen, Sara Lustigman
Vaccination remains the most cost-effective biomedical approach for controlling influenza disease. In times of pandemics, however, these vaccines cannot be produced in sufficient quantities for worldwide use by the current manufacturing capacities and practices. What is needed is the development of adjuvanted vaccines capable of inducing an adequate or better immune response at a decreased antigen dose. Previously we showed that the protein adjuvant rOv-ASP-1 augments influenza-specific antibody titers and survival after virus challenge in both young adult and old-age mice when administered with the trivalent inactivated influenza vaccine (IIV3)...
May 12, 2018: Vaccine
https://www.readbyqxmd.com/read/29738575/adoptive-transfer-of-dendritic-cells-expressing-cd11c-reduces-the-immunological-response-associated-with-experimental-colitis-in-balb-c-mice
#4
Lisiery N Paiatto, Fernanda G D Silva, Áureo T Yamada, Wirla M S C Tamashiro, Patricia U Simioni
INTRODUCTION: In addition to conventional therapies, several new strategies have been proposed for modulating autoimmune diseases, including the adoptive transfer of immunological cells. In this context, dendritic cells (DCs) appear to be one of the most promising treatments for autoimmune disorders. The present study aimed to evaluate the effects of adoptive transfer of DCs obtained from both naïve and ovalbumin (OVA)-tolerant mice on the severity of TNBS induced colitis and analyze the eventual protective mechanisms...
2018: PloS One
https://www.readbyqxmd.com/read/29729115/tissue-expression-of-aquaporine-3-in-different-sites-of-vitiligo-an-immunohistochemical-study
#5
S M Esmat, O A Zeid, D M Abdel Halim, M T Fawzy, M Abdel Latif, V Lazarova, M Assaf
The role of epidermal keratinocytes in the pathogenesis of vitiligo has been ignored despite the fact that both melanocytes and keratinocytes form one functional and structural unit. Aquaporin3 (AQP3), a glycerol water channel expressed in epithelial tissue, is involved in the regulation of keratinocyte proliferation, cell migration, and has a potential role in hypopigmentation in vitiligo. Normally, acral skin differs significantly from non-acral skin. Acral skin shows significantly lower hair follicle density, melanocyte density, Langerhans cell (LC) density, epidermal MHCII expression...
May 5, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29712687/cross-talk-between-t-cells-and-hematopoietic-stem-cells-during-adoptive-cellular-therapy-for-malignant-glioma
#6
Tyler J Wildes, Adam Grippin, Kyle A Dyson, Brandon M Wummer, David J Damiani, Rebecca S Abraham, Catherine Flores, Duane A Mitchell
PURPOSE: Adoptive T cell immunotherapy (ACT) has emerged as a viable therapeutic for peripheral and central nervous system (CNS) tumors. In peripheral cancers, optimal efficacy of ACT is reliant on dendritic cells (DCs) in the tumor microenvironment. However, the CNS is largely devoid of resident migratory DCs to function as antigen-presenting cells during immunotherapy. Herein, we demonstrate that cellular interactions between adoptively-transferred tumor-reactive T cells and bone marrow-derived HSPCs lead to the generation of potent intratumoral DCs within the CNS compartment...
April 30, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29697861/microglial-mhc-class-ii-is-dispensable-for-experimental-autoimmune-encephalomyelitis-and-cuprizone-induced-demyelination
#7
Yochai Wolf, Anat Shemer, Liron Levy-Efrati, Mor Gross, Jung-Seok Kim, Adrien Engel, Eyal David, Louise Chappell-Maor, Jonathan Grozovski, Ron Rotkopf, Inbal Biton, Raya Eilam-Altstadter, Steffen Jung
Microglia are resident immune cells in the central nervous system (CNS), strategically positioned to clear dead cells and debris, and orchestrate CNS inflammation and immune defense. In steady state, these macrophages lack major histocompatibility complex class II (MHCII) expression, but microglia activation can be associated with MHCII induction. Whether microglial MHCII serves antigen presentation for critical local T cell re-stimulation in CNS auto-immune disorders or modulates microglial signaling output remains under debate...
April 26, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29682352/optimization-of-ex-vivo-murine-bone-marrow-derived-immature-dendritic-cells-a-comparative-analysis-of-flask-culture-method-and-mouse-cd11c-positive-selection-kit-method
#8
Rahul Ashok Gosavi, Sukeshani Salwe, Sandeepan Mukherjee, Ritwik Dahake, Sweta Kothari, Vainav Patel, Abhay Chowdhary, Ranjana A Deshmukh
12-14 days of culturing of bone marrow (BM) cells containing various growth factors is widely used method for generating dendritic cells (DCs) from suspended cell population. Here we compared flask culture method and commercially available CD11c Positive Selection kit method. Immature BMDCs' purity of adherent as well as suspended cell population was generated in the decreasing concentration of recombinant-murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF) in nontreated tissue culture flasks...
2018: Bone Marrow Research
https://www.readbyqxmd.com/read/29679380/a-non-myeloablative-chimeric-mouse-model-accurately-defines-microglia-and-macrophage-contribution-in-glioma
#9
Kenny Yu, A Saam Youshani, Fiona L Wilkinson, Claire O'Leary, Peter Cook, Liisi Laaniste, Aiyin Liao, Dominic Mosses, Christopher Waugh, Hannah Shorrock, Omar Pathmanaban, Andrew Macdonald, Ian Kamaly-Asl, Federico Roncaroli, Brian W Bigger
AIMS: Resident and peripherally-derived glioma associated microglia/macrophages (GAMM) play a key role in driving tumour progression, angiogenesis, invasion, and attenuating host immune responses. Differentiating these cells' origins is challenging and current pre-clinical models such as irradiation-based adoptive transfer, parabiosis and transgenic mice have limitations. We aimed to develop a novel non-myeloablative transplantation (NMT) mouse model that permits high levels of peripheral chimerism without blood-brain barrier (BBB) damage or brain infiltration prior to tumour implantation...
April 20, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29666605/can-a-proper-t-cell-development-occur-in-an-altered-thymic-epithelium-lessons-from-ephb-deficient-thymi
#10
Juan José Muñoz, Javier García-Ceca, Sara Montero-Herradón, Beatriz Sánchez Del Collado, David Alfaro, Agustín Zapata
For a long time, the effects of distinct Eph tyrosine kinase receptors and their ligands, ephrins on the structure, immunophenotype, and development of thymus and their main cell components, thymocytes (T) and thymic epithelial cells (TECs), have been studied. In recent years, the thymic phenotype of mutant mice deficient in several Ephs and ephrins B has been determined. Remarkably, thymic stroma in these animals exhibits important defects that appear early in ontogeny but little alterations in the proportions of distinct lymphoid cell populations...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29662488/subretinal-injection-of-hy-peptides-induces-systemic-antigen-specific-inhibition-of-effector-cd4-and-cd8-t-cell-responses
#11
Julie Vendomèle, Safa Dehmani, Quentin Khebizi, Anne Galy, Sylvain Fisson
Purpose: Injection of an antigen into the anterior chamber of the eye induces a peripheral antigen-specific immune modulation mechanism, known as anterior chamber-associated immune deviation (ACAID). Delayed-type hypersensitivity experiments argue that the subretinal space (SR) of the eye displays properties similar to ACAID. However, no investigation was performed regarding the differential impact of a subretinal antigen injection on peripheral CD4+ versus CD8+ T cells, on the potential immune deviation regarding Th profiles, and on the antigen-specificity of the inhibition...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29642539/in-silico-functional-networks-identified-in-fish-nucleated-red-blood-cells-by-means-of-transcriptomic-and-proteomic-profiling
#12
Sara Puente-Marin, Iván Nombela, Sergio Ciordia, María Carmen Mena, Verónica Chico, Julio Coll, María Del Mar Ortega-Villaizan
Nucleated red blood cells (RBCs) of fish have, in the last decade, been implicated in several immune-related functions, such as antiviral response, phagocytosis or cytokine-mediated signaling. RNA-sequencing (RNA-seq) and label-free shotgun proteomic analyses were carried out for in silico functional pathway profiling of rainbow trout RBCs. For RNA-seq, a de novo assembly was conducted, in order to create a transcriptome database for RBCs. For proteome profiling, we developed a proteomic method that combined: (a) fractionation into cytosolic and membrane fractions, (b) hemoglobin removal of the cytosolic fraction, (c) protein digestion, and (d) a novel step with pH reversed-phase peptide fractionation and final Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometric (LC ESI-MS/MS) analysis of each fraction...
April 9, 2018: Genes
https://www.readbyqxmd.com/read/29627302/inhibitive-effect-of-resveratrol-on-the-inflammation-in-cultured-astrocytes-and-microglia-induced-by-a%C3%AE-1-42
#13
Haifeng Zhao, Qian Wang, Xuejiao Cheng, Xuemin Li, Na Li, Tiantian Liu, Jing Li, Qian Yang, Ruirui Dong, Yusen Zhang, Luping Zhang
Astrocytes and microglia appear central to the initiation and progression of neuroinflammation in Alzheimer's disease (AD). In this study, inflammation was mimicked by Aβ1-42 treatment of rat astrocytes (RA) and N9 microglia cell lines. Inflammation induced by Aβ1-42 can be inhibited by pyrrolidinedithiocarbamicacid (PDTC), indicating that the NF-κB signal pathway is involved in the inflammation. Then, the inhibitive effects of resveratrol (Res) on the inflammation in RA and N9 cells were assessed by observing the changes in inflammatory factors, chemokines, cell cycle and adhesion molecules on the cell surface...
April 5, 2018: Neuroscience
https://www.readbyqxmd.com/read/29599140/follicular-b-cells-promote-atherosclerosis-via-t-cell-mediated-differentiation-into-plasma-cells-and-secreting-pathogenic-immunoglobulin-g
#14
Christopher Tay, Yu-Han Liu, Peter Kanellakis, Axel Kallies, Yi Li, Anh Cao, Hamid Hosseini, Peter Tipping, Ban-Hock Toh, Alex Bobik, Tin Kyaw
OBJECTIVE: B cells promote or protect development of atherosclerosis. In this study, we examined the role of MHCII (major histocompatibility II), CD40, and Blimp-1 (B-lymphocyte-induced maturation protein) expression by follicular B (FO B) cells in development of atherosclerosis together with the effects of IgG purified from atherosclerotic mice. APPROACH AND RESULTS: Using mixed chimeric Ldlr -/- mice whose B cells are deficient in MHCII or CD40, we demonstrate that these molecules are critical for the proatherogenic actions of FO B cells...
March 29, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29584685/characterization-of-human-type-c-enterotoxin-produced-by-clinical-s-epidermidis-isolates
#15
Chimène Nanoukon, Dissou Affolabi, Daniel Keller, Rodrigue Tollo, Philippe Riegel, Lamine Baba-Moussa, Gilles Prévost
Staphylococcal Enterotoxins (SEs) are superantigens (SAg) originally produced by S. aureus , but their presence in coagulase negative staphylococci (CNS) has long been suspected. This study aims to better characterize a novel C-like enterotoxin expressed by clinical S. epidermidis strains, called SECepi . We isolated and characterized SECepi for its molecular and functional properties. The toxin was structurally modeled according to its significant similarity with S. aureus SEC3. Most of SEC amino acid residues important for the formation of the trimolecular Major Histocompatibility Complex II MHCII-SEC-T Cell Receptor TCR complex are conserved in SECepi ...
March 27, 2018: Toxins
https://www.readbyqxmd.com/read/29574785/resident-alveolar-macrophages-are-master-regulators-of-arrested-alveolarization-in-experimental-bronchopulmonary-dysplasia
#16
Tatiana V Kalymbetova, Balachandar Selvakumar, José Alberto Rodríguez-Castillo, Miša Gunjak, Christina Malainou, Miriam Ruth Heindl, Alena Moiseenko, Cho-Ming Chao, István Vadász, Konstantin Mayer, Jürgen Lohmeyer, Saverio Bellusci, Eva Böttcher-Friebertshäuser, Werner Seeger, Susanne Herold, Rory E Morty
Trophic functions for macrophages are emerging as key mediators of developmental processes; including bone, vessel, and mammary gland development. Yolk sac-derived macrophages mature in the distal lung shortly after birth. Myeloid-lineage macrophages are recruited to the lung and are activated under pathological conditions. These pathological conditions include bronchopulmonary dysplasia (BPD), a common complication of preterm birth characterized by stunted lung development, where formation of alveoli is blocked...
March 25, 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29569714/unexpected-involvement-of-il-13-signalling-via-a-stat6-independent-mechanism-during-murine-igg2a-development-following-viral-vaccination
#17
Megat A Hamid, Ronald J Jackson, Sreeja Roy, Mayank Khanna, Charani Ranasinghe
In this study, recombinant pox viral vaccination was shown to induce highly elevated IgG2a and low IgG1 antibody expression in mice lacking IL-4 or STAT6, whilst IL-13-/- mice exhibited elevated IgG1, but very low IgG2a. These findings revealed that IL-13 and IL-4 differentially regulated antibody development. To understand this further, when STAT6-/- mice were given a vaccine co-expressing IL-13Rα2 that temporarily sequestered IL-13, significantly reduced IgG2a expression, was detected. These findings for the first time demonstrated that IL-13 regulated IgG2a differentiation utilising an alternative IL-13R signalling pathway independent of STAT6 (IL-13Rα2 pathway)...
March 23, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/29567418/plasticizer-di-2-ethylhexyl-phthalate-dehp-enhances-cockroach-allergen-extract-driven-airway-inflammation-by-enhancing-pulmonary-th2-as-well-as-th17-immune-responses-in-mice
#18
Ali S Alfardan, Ahmed Nadeem, Sheikh F Ahmad, Naif O Al-Harbi, Mohammad M Al-Harbi, Shakir D AlSharari
In recent decades, there has been a gradual increase in the prevalence of asthma. Various factors including environmental pollutants have contributed to this phenomenon. Plasticizer, di(2-ethylhexyl)phthalate (DEHP) is one of the commonest environmental pollutants due to its association with plastic products. DEHP gets released from plastic products easily leading to respiratory exposure in humans. As a consequence, DEHP is associated with allergic asthma in humans and animals. DEHP is reported to act as an adjuvant in ovalbumin-induced mouse models of asthma at high doses...
March 19, 2018: Environmental Research
https://www.readbyqxmd.com/read/29549257/calcineurin-mediated-il-2-production-by-cd11c-high-mhcii-myeloid-cells-is-crucial-for-intestinal-immune-homeostasis
#19
Andrea Mencarelli, Hanif Javanmard Khameneh, Jan Fric, Maurizio Vacca, Sary El Daker, Baptiste Janela, Jing Ping Tang, Sabrina Nabti, Akhila Balachander, Tong Seng Lim, Florent Ginhoux, Paola Ricciardi-Castagnoli, Alessandra Mortellaro
The intestinal immune system can respond to invading pathogens yet maintain immune tolerance to self-antigens and microbiota. Myeloid cells are central to these processes, but the signaling pathways that underlie tolerance versus inflammation are unclear. Here we show that mice lacking Calcineurin B in CD11chigh MHCII+ cells (Cnb1 CD11c mice) spontaneously develop intestinal inflammation and are susceptible to induced colitis. In these mice, colitis is associated with expansion of T helper type 1 (Th1) and Th17 cell populations and a decrease in the number of FoxP3+ regulatory T (Treg) cells, and the pathology is linked to the inability of intestinal Cnb1-deficient CD11chigh MHCII+ cells to express IL-2...
March 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29546878/inflammation-induces-two-types-of-inflammatory-dendritic-cells-in-inflamed-lymph-nodes
#20
Jiyoun Min, Dongchan Yang, Mirang Kim, Keeok Haam, Anji Yoo, Jae-Hoon Choi, Barbara U Schraml, Yong Sung Kim, Dongsup Kim, Suk-Jo Kang
The spatiotemporal regulation of immune cells in lymph nodes (LNs) is crucial for mounting protective T-cell responses, which are orchestrated by dendritic cells (DCs). However, it is unclear how the DC subsets are altered by the inflammatory milieu of LNs. Here, we show that the inflamed LNs of Listeria-infected mice are characterized by the clustering of neutrophils and monocytes and IFN-γ production. Significantly, the early inflammatory responses are coupled with the differentiation of not one, but two types of CD64+ CD11c+ MHCII+ inflammatory DCs...
March 16, 2018: Experimental & Molecular Medicine
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