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https://www.readbyqxmd.com/read/29933224/a-molecularly-imprinted-electrochemiluminescence-sensor-for-ultrasensitive-hiv-1-gene-detection-using-eus-nanocrystals-as-luminophore
#1
Bahareh Babamiri, Abdollah Salimi, Rahman Hallaj
Development of simple, sensitive and specific method for human immunodeficiency virus (HIV) assays are urgently demand. In this study, we developed a novel molecularly imprinted polymer (MIP) electrochemiluminescence (MIP-ECL) sensor for the highly sensitive and selective HIV-1 gene detection using Europium sulfide nanocrystals (EsNCs) as signal producing compound. Here, the HIV aptamer as the template and o-phenylenediamine as the functional monomer, were electropolymerized directly on the surface of ITO electrode...
June 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29933161/highly-efficient-extraction-of-lead-ions-from-smelting-wastewater-slag-and-contaminated-soil-by-two-dimensional-montmorillonite-based-surface-ion-imprinted-polymer-absorbent
#2
Chao Zhu, Tianjue Hu, Lin Tang, Guangming Zeng, Yaocheng Deng, Yue Lu, Siyuan Fang, Jingjing Wang, Yani Liu, Jiangfang Yu
The rapid, efficient and selective extraction of heavy metal ions is significant for wastewater pretreatment and metal ion recycle. Using montmorillonite as the template substrate and Pb2+ as template ions, a novel two-dimensional montmorillonite-based surface ion imprinted polymer (IIP-MMT) adsorbent is successfully synthesized via activators generated by electron transfer for atom transfer radical polymerization (AGET-ATRP). Batch adsorption experiments are performed to assess the properties of the imprinted polymer sorbent, along with its selectivity and reusability in practical extraction of Pb2+ ...
June 15, 2018: Chemosphere
https://www.readbyqxmd.com/read/29932725/imprints-of-the-molecular-electronic-structure-in-the-photoelectron-spectra-of-strong-field-ionized-asymmetric-triatomic-model-molecules
#3
Matthias Paul, Lun Yue, Stefanie Gräfe
We examine the circular dichroism in the angular distribution of photoelectrons of triatomic model systems ionized by strong-field ionization. Following our recent work on this effect [Paul, Yue, and Gräfe, J. Mod. Opt. 64, 1104 (2017)JMOPEW0950-034010.1080/09500340.2017.1299883], we demonstrate how the symmetry and electronic structure of the system is imprinted into the photoelectron momentum distribution. We use classical trajectories to reveal the origin of the threefolded pattern in the photoelectron momentum distribution, and show how an asymmetric nuclear configuration of the triatomic system effects the photoelectron spectra...
June 8, 2018: Physical Review Letters
https://www.readbyqxmd.com/read/29932057/in-praise-of-mealybugs
#4
Vani Brahmachari, Surbhi Kohli, Parul Gulati
The fascinating chromosomal cycle leading to facultative heterochromatization in the mealybugs has been a challenging system for mechanistic understanding of the phenomenon of genomic imprinting and epigenetics. The elegant cytological dissection of the various processes reported in the literature is equally fascinating for the researchers of current molecular age. Presently, a two way approach is being pursued; continued efforts of utilizing elegant cytology, in combination with the molecular probes to decipher molecular correlates on one hand and on the other, the de novo biochemical/molecular analysis for the identification of the molecular players using genomic tools...
June 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29932056/epigenetics-with-special-reference-to-the-human-x-chromosome-inactivation-and-the-enigma-of-drosophila-dna-methylation
#5
Deepti Deobagkar
Epigenetics confers adaptability and survival advantage to an organism. Most epigenetic processes demonstrate memory and heritability. DNA methylation is an epigenetic process that adds imprints which can be inherited during cell division and across generations. DNA methylation adds an additional level of information to the basic DNA sequence and can influence chromatin organization and the function of the DNA sequence. In bacteria, it works as a defence strategy and preserves genome integrity. DNA methylation in eukaryotes has been implicated in a large number of cellular regulatory processes and is implied in development, differentiation, life style diseases and cancer...
June 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29931170/a-tandem-repeat-array-in-ig-dmr-is-essential-for-imprinting-of-paternal-allele-at-the-dlk1-dio3-domain-during-embryonic-development
#6
Takeshi Saito, Satoshi Hara, Tomoko Kato, Moe Tamano, Akari Muramatsu, Hiroshi Asahara, Shuji Takada
Genomic imprinting is a phenomenon that causes parent-origin-specific monoallelic expression of a small subset of genes, known as imprinted genes, by parentally inherited epigenetic marks. Imprinted genes at the delta-like homolog 1 gene (Dlk1)-type III iodothyronine deiodinase gene (Dio3) imprinted domain, regulated by intergenic differentially methylated region (IG-DMR), are essential for normal development of late embryonic stages. Although the functions of IG-DMR have been reported by generating knockout mice, molecular details of the regulatory mechanisms are not fully understood as the specific sequence(s) of IG-DMR have not been identified...
June 20, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29931015/molecularly-imprinted-electrospun-nanofibers-for-adsorption-of-2-4-dinitrotoluene-in-water
#7
Xiaoting Xue, Rui Lu, Yi Li, Qing Wang, Jiansheng Li, Lianjun Wang
The detection and adsorption of nitroaromatic compounds such as 2,4-dinitrotoluene (DNT) is of great importance, but the selective detection of trace DNT in water remains a challenge. Here, we report molecularly imprinted polymer (MIP) nanofibers fabricated by electrospinning using DNT as a template molecule and poly(allylamine) as a functional macromer. The physical and chemical properties of the nanofibers were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM), and the DNT adsorption and selectivity properties were also studied by high performance liquid chromatography (HPLC)...
June 22, 2018: Analyst
https://www.readbyqxmd.com/read/29930742/multifactorial-analysis-of-the-stochastic-epigenetic-variability-in-cord-blood-confirmed-an-impact-of-common-behavioral-and-environmental-factors-but-not-of-in-vitro-conception
#8
D Gentilini, E Somigliana, L Pagliardini, E Rabellotti, P Garagnani, L Bernardinelli, E Papaleo, M Candiani, A M Di Blasio, P Viganò
Background: An increased incidence of imprint-associated disorders has been reported in babies born from assisted reproductive technology (ART). However, previous studies supporting an association between ART and an altered DNA methylation status of the conceived babies have been often conducted on a limited number of methylation sites and without correction for critical potential confounders. Moreover, all the previous studies focused on the identification of methylation changes shared among subjects while an evaluation of stochastic differences has never been conducted...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29929216/molecularly-imprinted-covalent-organic-polymers-for-the-selective-extraction-of-benzoxazole-fluorescent-whitening-agents-from-food-samples
#9
Hui Ding, Rongyu Wang, Xiao Wang, Wenhua Ji
Molecularly imprinted covalent organic polymers were constructed by an imine-linking reaction between 1,3,5-triformylphloroglucinol and 2,6-diaminopyridine and used for the selective solid-phase extraction of benzoxazole fluorescent whitening agents from food samples. Binding experiments showed that imprinting sites on molecularly imprinted polymers had higher selectivity for targets compared with those of the corresponding non-imprinted polymers. Parameters affecting the solid-phase extraction procedure were examined...
June 21, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29928916/improving-phospholipase-d-activity-and-selectivity-by-bio-imprinting-immobilization-to-produce-phosphatidylglycerol
#10
Binglin Li, Dandan Duan, Jiao Wang, Huanyu Li, Xiaoli Zhang, Binxia Zhao
Phospholipase D (PLD) was firstly hyperactivated by the bio-imprinting and, then, these hyperactivated PLD molecules were immobilized by adsorption and precipitation, followed by cross-linking. The high degree of conformational rigidification provided by intra- and intermolecular cross-linking can make PLD "remember" imprint-induced characteristics even in aqueous solutions. The obtained immobilized PLD showed the excellent catalytic performance. The maximum activity of immobilized PLD reached 166953 U/gprotein , which was approximately 14 times higher than that of free form (11922 U/gprotein )...
June 18, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29928277/gestational-hypothyroxinemia-affects-its-offspring-with-a-reduced-suppressive-capacity-impairing-the-outcome-of-the-experimental-autoimmune-encephalomyelitis
#11
Henny Haensgen, Eduardo Albornoz, María C Opazo, Katherinne Bugueño, Evelyn Liliana Jara Fernández, Rebecca Binzberger, Tomás Rivero-Castillo, Luis F Venegas Salas, Felipe Simon, Claudio Cabello-Verrugio, Alvaro A Elorza, Alexis M Kalergis, Susan M Bueno, Claudia A Riedel
Hypothyroxinemia (Hpx) is a thyroid hormone deficiency (THD) condition highly frequent during pregnancy, which although asymptomatic for the mother, it can impair the cognitive function of the offspring. Previous studies have shown that maternal hypothyroidism increases the severity of experimental autoimmune encephalomyelitis (EAE), an autoimmune disease model for multiple sclerosis (MS). Here, we analyzed the immune response after EAE induction in the adult offspring gestated in Hpx. Mice gestated in Hpx showed an early appearance of EAE symptoms and the increase of all parameters of the disease such as: the pathological score, spinal cord demyelination, and immune cell infiltration in comparison to the adult offspring gestated in euthyroidism...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29927602/coarse-grained-simulation-of-protein-imprinted-hydrogels
#12
Israel Zadok, Simcha Srebnik
The MARTINI force-field is adapted for acrylic functional monomers and used to simulate protein-imprinted polymers, including complexation in solution, cross-linking reaction, washing, and rebinding of the protein. A globally-controlled grand canonical Monte Carlo simulation combined with molecular dynamics is used to achieve proper hydration and swelling of the gel. Two gel formulations are compared, one with only polar functional monomers, and one with additional small number of charged monomers. Imprinted hydrogels are evaluated for two proteins, lysozyme and cytochrome C, chosen due to their similarity in size and isoelectric point, though it is known that their imprinting behavior is very different...
June 21, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29927061/the-key-role-of-behaviour-in-animal-camouflage
#13
Martin Stevens, Graeme D Ruxton
Animal camouflage represents one of the most important ways of preventing (or facilitating) predation. It attracted the attention of the earliest evolutionary biologists, and today remains a focus of investigation in areas ranging from evolutionary ecology, animal decision-making, optimal strategies, visual psychology, computer science, to materials science. Most work focuses on the role of animal morphology per se, and its interactions with the background in affecting detection and recognition. However, the behaviour of organisms is likely to be crucial in affecting camouflage too, through background choice, body orientation and positioning; and strategies of camouflage that require movement...
June 21, 2018: Biological Reviews of the Cambridge Philosophical Society
https://www.readbyqxmd.com/read/29926809/enhancing-adsorption-capacity-while-maintaining-specific-recognition-performance-of-mesoporous-silica-a-novel-imprinting-strategy-with-amphiphilic-ionic-liquid-as-surfactant
#14
Shichao Ding, Zhiling Li, Yuan Cheng, Chunbao Du, Junfeng Gao, Yong-Wei Zhang, Nan Zhang, Zhaotong Li, Ninghui Chang, Xiaoling Hu
In order to facilitate the broad applications of molecular recognition materials in biomedical areas, it is critical to enhance their adsorption capacity while maintaining their excellent recognition performance. In this work, we designed and synthesized well-defined peptide-imprinted mesoporous silica (PIMS) for specific recognition of an immunostimulating hexapeptide from human casein (IHHC) by using amphiphilic ionic liquid as the surfactant to anchor IHHC via a combination of one step sol-gel method and docking oriented imprinting approach...
June 21, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29923557/molecularly-imprinted-polymer-nanoparticle-based-assay-mina-application-for-fumonisin-b1-determination
#15
Hasim Munawar, Katarzyna Smolinska-Kempisty, Alvaro Garcia Cruz, Francesco Canfarotta, Elena Piletska, Khalku Karim, Sergey A Piletsky
The enzyme-linked immunosorbent assay (ELISA) has been used as a standard tool for monitoring food and animal feed contamination from the carcinogenic fumonisin B1 (FB1). Unfortunately, ELISA is not always efficient due to the instability of the antibody and enzyme components in the immunoassay, the presence of natural enzyme inhibitors in the samples and the high levels of non-specific protein binding. Additionally, the production of antibodies for ELISA can be time-consuming and costly, due to the involvement of animals in the manufacturing process...
June 20, 2018: Analyst
https://www.readbyqxmd.com/read/29922862/a-molecularly-imprinted-sensor-with-enzymatic-enhancement-of-electrochemiluminescence-of-quantum-dots-for-ultratrace-clopyralid-determination
#16
Qingyu Wang, Shuhuai Li, Jianping Li
A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. During the analytical cycle, horseradish peroxidase (HRP)-labeled CPD was replaced by CPD in the sample...
June 19, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29922638/-de-novo-duplication-of-11p15-associated-with-congenital-diaphragmatic-hernia
#17
Gabriel C Dworschak, Hartmut Engels, Jessica Becker, Lukas Soellner, Thomas Eggermann, Florian Kipfmueller, Andreas Müller, Heiko Reutter, Martina Kreiß
Background: Congenital diaphragmatic hernia (CDH) is a rare defect of the diaphragm commonly associated with high morbidity and mortality due to lung hypoplasia and pulmonary hypertension. Although in 70% of patients the etiology of a CDH remains unknown, a multitude of causative chromosomal aberrations has been identified. Case presentation: We describe the first case of isolated 11p15 duplication with CDH. The 18.6 Mb large duplication affected 285 RefSeq genes and included the Beckwith-Wiedemann (BWS)-associated imprinting control region 2 (ICR2, KCNQ1OT1 TSS DMR), whereas the ICR1 ( H19 TSS DMR) was not affected...
2018: Frontiers in Pediatrics
https://www.readbyqxmd.com/read/29921775/the-type-3-deiodinase-epigenetic-control-of-brain-thyroid-hormone-action-and-neurological-function
#18
REVIEW
Arturo Hernandez, J Patrizia Stohn
Thyroid hormones (THs) influence multiple processes in the developing and adult central nervous system, and their local availability needs to be maintained at levels that are tailored to the requirements of their biological targets. The local complement of TH transporters, deiodinase enzymes, and receptors is critical to ensure specific levels of TH action in neural cells. The type 3 iodothyronine deiodinase (DIO3) inactivates THs and is highly present in the developing and adult brain, where it limits their availability and action...
June 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29920665/the-evolution-of-sexual-imprinting-as-an-assortative-mating-mechanism
#19
Kwan Lung Elroy Au
In this issue, Yeh et al. (2018) investigated whether sexual imprinting could act as an assortative mating mechanism, reducing hybridization and increasing premating isolation. While they indeed find that imprinting leads to assortative mating and reduced hybridization, the strength at which imprinting evolves is usually intermediate, because it is counterbalanced by the costs of imprinting and the benefits of adaptive hybridization. Thus, while sexual imprinting can act as an assortative mating mechanism, it is often not the sole element of female mate choice...
June 19, 2018: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/29920607/seasonal-changes-of-%C3%AE-d-and-%C3%AE-18o-in-tree-ring-cellulose-of-quercus-crispula-suggest-a-change-in-post-photosynthetic-processes-during-earlywood-growth
#20
Eri Nabeshima, Takeshi Nakatsuka, Akira Kagawa, Tsutom Hiura, Ryo Funada
Leaf photosynthetic and post-photosynthetic processes modulate the isotope ratios of tree-ring cellulose. Post-photosynthetic processes, such as the remobilization of stored starch in early spring, are important to understanding the mechanisms of xylem formation in tree stems; however, untangling the isotope ratio signals of photosynthetic and post-photosynthetic processes imprinted on tree rings is difficult. Portions of carbon-bound hydrogen and oxygen atoms are exchanged with medium water during post-photosynthetic processes...
June 15, 2018: Tree Physiology
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