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https://www.readbyqxmd.com/read/27935290/aromatic-functionality-of-target-proteins-influences-monomer-selection-for-creating-artificial-antibodies-on-plasmonic-biosensors
#1
Rong Hu, Jingyi Luan, Evan D Kharasch, Srikanth Singamaneni, Jeremiah J Morrissey
Natural antibodies used as biorecognition elements suffer from numerous shortcomings, such as limited chemical and environmental stability and cost. Artificial antibodies based on molecular imprinting are an attractive alternative to natural antibodies. We investigated the role of aromatic interactions in target recognition capabilities of artificial antibodies. Three proteins with different aromatic amino acid content were employed as model targets. Artificial antibodies were formed on nanostructures using combinations of silane monomers of varying aromatic functionality...
December 9, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27935252/innovative-method-for-the-enrichment-of-high-polarity-bioactive-molecules-present-at-low-concentrations-in-complex-matrices
#2
Qing-Shan Liu, Jie He, Wen-Bin Zhou, Yu-Long Gu, Ke-Qin Li, Xiao-Ying Yin
Ginsenoside Rg1 is a valuable bioactive molecule but its high polarity and low concentration in complex mixtures makes it a challenge to separate Ginsenoside Rg1 from other saponins with similar structures, resulting in low extraction efficiency. The successful development of effective Rg1 molecularly imprinted polymers that exhibit high selectivity and adsorption may offer an improved method for the enrichment of active compounds. In this work, molecularly imprinted polymers were prepared with two different methods, precipitation polymerization or surface imprinted polymerization...
December 9, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27933499/assessment-of-molecularly-imprinted-polymers-mips-in-the-preconcentration-of-disperse-red-73-dye-prior-to-photoelectrocatalytic-treatment
#3
Jefferson Honorio Franco, Alejandra Ben Aissa, Guilherme Garcia Bessegato, Laura Martinez Fajardo, Maria Valnice Boldrin Zanoni, María Isabel Pividori, Maria Del Pilar Taboada Sotomayor
Magnetic molecularly imprinted polymers (MMIPs) have become a research hotspot due to their two important characteristics: target recognition and magnetic separation. This paper presents the preparation, characterization, and optimization of an MMIP for the preconcentration of disperse red 73 dye (DR73) and its subsequent efficient degradation by photoelectrocatalytic treatment. The MMIPs were characterized by scanning electron microscopy (SEM), which revealed homogeneous distribution of the particles. Excellent encapsulation of magnetite was confirmed by transmission electron microscopy (TEM)...
December 8, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27933046/phylogenetic-signals-of-salinity-and-season-in-bacterial-community-composition-across-the-salinity-gradient-of-the-baltic-sea
#4
Daniel P R Herlemann, Daniel Lundin, Anders F Andersson, Matthias Labrenz, Klaus Jürgens
Understanding the key processes that control bacterial community composition has enabled predictions of bacterial distribution and function within ecosystems. In this study, we used the Baltic Sea as a model system to quantify the phylogenetic signal of salinity and season with respect to bacterioplankton community composition. The abundances of 16S rRNA gene amplicon sequencing reads were analyzed from samples obtained from similar geographic locations in July and February along a brackish to marine salinity gradient in the Baltic Sea...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27932011/child-physical-development-in-the-uk-the-imprint-of-time-and-socioeconomic-status
#5
B H Apouey
OBJECTIVES: Social health inequalities remain a key policy challenge. The existing literature has not presented a synthetic view on the evolution of inequalities in physical development across childhood. We examine social disparities as children grow older using a range of different outcomes. STUDY DESIGN: Population-based secondary data analysis. METHODS: We employ longitudinal data on British children aged 9 months to 12 years from the Millennium Cohort Study (n = 13,811-18,987) and focus on multiple child physical measures: weight, body mass index (BMI), overweight, fat mass and waist circumference...
December 2016: Public Health
https://www.readbyqxmd.com/read/27931036/clinical-exome-sequencing-reveals-mkrn3-pathogenic-variants-in-familial-and-nonfamilial-idiopathic-central-precocious-puberty
#6
Nelmar Valentina Ortiz-Cabrera, Rosa Riveiro-Álvarez, Miguel Ángel López-Martínez, Pilar Pérez-Segura, Isabel Aragón-Gómez, María José Trujillo-Tiebas, Leandro Soriano-Guillén
BACKGROUND/AIMS: Idiopathic central precocious puberty (ICPP) is the premature activation of the hypothalamic-pituitary-gonadal axis in the absence of organic disease. Up to now, just gain-of-function mutations of KISS1/KISS1R and loss-of-function mutations of the maternally imprinted gene MKRN3 are the known genetic causes of ICPP. Our intention is to evaluate variants present in genes related to the pubertal onset pathway that could act as disease-causing or predisposing variants. METHODS: We studied the clinical exome of 20 patients diagnosed with ICPP using the Illumina platform...
December 9, 2016: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/27928889/synthesis-of-a-molecularly-imprinted-polymer-for-the-selective-recognition-of-carmoisine-azorubin-e122-from-pomegranate-juice
#7
Zahra Ghasempour, Mohammad Alizadeh-Khaledabad, Mohammad-Reza Vardast, Mahmoud Rezazad- Bari
Since natural pigments are lost during the processing of beverages like pomegranate juice, carmoisine, as an adulterant, is often added into the pure juice to improve color characteristics. In this study, molecularly imprinted polymers, as adsorbent of carmoisine, were synthesized using acrylamide, methacrylic acid and 4-vinylpyridine as functional monomers and then they were evaluated in terms of separation and detection of carmoisine. Experiments on batch adsorption of carmoisine 10 ppm stock solution revealed a better binding capacity for 4-vinylpyridine based polymer in comparison to methacrylic acid and acrylamide polymers...
December 8, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27928531/a-simple-and-fast-method-based-on-new-magnetic-ion-imprinted-polymer-as-a-highly-selective-sorbent-for-preconcentration-and-determination-of-cadmium-in-environmental-samples
#8
Zahra Panjali, Ali Akbar Asgharinezhad, Homeira Ebrahimzadeh, Niloofar Jalilian, Rasoul Yarahmadi, Seyed Jamaleddin Shahtaheri
BACKGROUND: The analysis of heavy metals at trace level is one of the main toxicologists concern, due to their vital rules in human`s life. Cadmium is one of these toxic heavy metals, which released to the environment from various industries. METHODS: In order to determine Cd (II) ions in various matrices magnetic ion-imprinted polymer (IIP) method has been developed and applied. This nano-sorbent has been synthesized by coating an IIP compound on Fe3O4 nanoparticles core to achieve highest surface area...
August 2016: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/27925383/molecular-imprinting-for-substrate-selectivity-and-enhanced-activity-of-enzyme-mimics
#9
Zijie Zhang, Biwu Liu, Juewen Liu
Using molecular imprinting, a peroxidase-mimicking DNAzyme has achieved substrate specificity with enhanced activity in a nanoscale gel for three different substrates.
December 7, 2016: Small
https://www.readbyqxmd.com/read/27925264/three-intronic-lncrnas-with-monoallelic-expression-derived-from-the-meg8-gene-in-cattle
#10
W Yang, D Li, G Wang, C Zhang, M Zhang, W Zhang, S Li
The field of long noncoding RNA (lncRNA) research has been rapidly advancing in recent years. Antisense lncRNAs, intergenetic lncRNAs and enhancer lncRNAs can regulate genomic imprinting, which leads to parent-origin-specific monoalletic expression of genes. However, the function of intronic ncRNAs in genomic imprinting remains unclear. Previously, we obtained the cDNA sequence of cattle MEG8 gene, which is located in the DLK1-DIO3 imprinted clusters of cattle chromosome 21. In this study, we undertook a systematic search for transcripts mapping to the MEG8 intronic region and identified three novel lncRNAs, named MEG8 intronic RNA 1 (MEG8-IT1), MEG8 intronic RNA 2 (MEG8-IT2) and MEG8 intronic RNA 3 (MEG8-IT3) according to the GENCODE annotated bibliography...
December 7, 2016: Animal Genetics
https://www.readbyqxmd.com/read/27924985/creating-bhb-imprinted-magnetic-nanoparticles-with-multiple-binding-sites
#11
Yanxia Li, Yiting Chen, Lu Huang, BenYong Lou, Guonan Chen
A kind of protein imprinted over magnetic Fe3O4@Au multifunctional nanoparticles (NPs) with multiple binding sites was synthesized and investigated. Magnetic Fe3O4@Au NPs as carrier materials were modified with 4-mercaptophenylboronic acid (MPBA) and mercaptopropionic acid (MPA) to introduce boronic acids and carboxyl groups. Using Bovine Hemoglobin (BHb) as a template, a polydopamine(PDA)-based molecular imprinted film was fabricated to produce a kind of magnetic molecularly imprinted nanoparticle (MMIP), possessing multiple binding sites with benzene-diol, amino groups, boronic acids and carboxyl groups...
December 7, 2016: Analyst
https://www.readbyqxmd.com/read/27924045/a-novel-method-for-the-simultaneous-identification-of-methylcytosine-and-hydroxymethylcytosine-at-a-single-base-resolution
#12
Yuki Kawasaki, Yukiko Kuroda, Isao Suetake, Shoji Tajima, Fumitoshi Ishino, Takashi Kohda
Since the discovery of oxidative demethylation of methylcytosine (mC) by Tet enzymes, an analytical method has been urgently needed that would enable the identification of mC and hydroxymethylcytosine (hmC) at the single base resolution level, because their roles in gene regulation are quite different from each other. However, the bisulfite sequencing method, the gold standard for DNA methylation analysis at present, does not distinguish them. Recently reported alternative methods, such as oxBS-seq and TAB-seq, are not even capable of determining mC and hmC simultaneously...
October 24, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27922643/a-kanamycin-sensor-based-on-an-electrosynthesized-molecularly-imprinted-poly-o-phenylenediamine-film-on-a-single-walled-carbon-nanohorn-modified-glassy-carbon-electrode
#13
Shuang Han, Bingqian Li, Ze Song, Sihao Pan, Zhichao Zhang, Hui Yao, Shuyun Zhu, Guobao Xu
A single-walled carbon nanohorn (SWCNH) has been used to construct a molecularly imprinted electrochemical sensor for the first time. Kanamycin, a widely used aminoglycoside antibiotic, is used as a representative analyte to test the detection strategy. The kanamycin sensor was constructed by the electropolymerization of a molecularly imprinted poly-o-phenylenediamine film on a SWCNH modified glassy carbon electrode. The sensor was investigated in the presence or absence of kanamycin by cyclic voltammetry to verify the changes in the redox peak currents of K3Fe(CN)6...
December 6, 2016: Analyst
https://www.readbyqxmd.com/read/27920589/dna-methylation-of-regulatory-regions-of-imprinted-genes-at-birth-and-its-relation-to-infant-temperament
#14
Bernard F Fuemmeler, Chien-Ti Lee, Adelheid Soubry, Edwin S Iversen, Zhiqing Huang, Amy P Murtha, Joellen M Schildkraut, Randy L Jirtle, Susan K Murphy, Cathrine Hoyo
BACKGROUND: DNA methylation of the differentially methylated regions (DMRs) of imprinted genes is relevant to neurodevelopment. METHODS: DNA methylation status of the DMRs of nine imprinted genes in umbilical cord blood leukocytes was analyzed in relation to infant behaviors and temperament (n = 158). RESULTS: MEG3 DMR levels were positively associated with internalizing (β = 0.15, P = 0.044) and surgency (β = 0.19, P = 0.018) behaviors, after adjusting for birth weight, gender, gestational age at birth, maternal age at delivery, race/ethnicity, education level, smoking status, parity, and a history of anxiety or depression...
2016: Genetics & Epigenetics
https://www.readbyqxmd.com/read/27920363/detection-of-imprinting-effects-for-qualitative-traits-on-x-chromosome-based-on-nuclear-families
#15
Ji-Yuan Zhou, Xiao-Ping You, Ran Yang, Wing Kam Fung
Methods for detecting imprinting effects have been developed primarily for autosomal markers. However, no method is available in the literature to test for imprinting effects on X chromosome. Therefore, it is necessary to suggest methods for detecting such imprinting effects. In this article, the parental-asymmetry test on X chromosome (XPAT) is first developed to test for imprinting for qualitative traits in the presence of association, based on family trios each with both parents and their affected daughter...
December 5, 2016: Statistical Methods in Medical Research
https://www.readbyqxmd.com/read/27919817/selective-adsorption-of-cr-vi-ions-from-aqueous-solutions-using-cr-6-imprinted-pebax-chitosan-go-aptes-nanofibrous-adsorbent
#16
Mohammadmahdi Etemadi, Saman Samadi, Shabnam Sharif Yazd, Pooya Jafari, Negin Yousefi, Majid Aliabadi
In the present study, a novel Cr(6+)-imprinted Pebax/chitosan/GO/APTES nanofibrous adsorbent (Cr(6+)-PCGA) was prepared and its performance was compared with PCGA nanofibers for selective sorption of Cr(VI) ions from aqueous solutions. The prepared nanofibers were characterized using FTIR, SEM and EDAX analysis. The influence of batch sorption experiments including GO/APTES content, pH, contact time, Cr(VI) initial concentration and temperature on the Cr(VI) sorption efficiency using synthesized nanofibers was investigated...
December 2, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27919305/a-new-model-for-parent-of-origin-effect-analyses-applied-to-brown-swiss-cattle-slaughterhouse-data
#17
I Blunk, M Mayer, H Hamann, N Reinsch
Genomic imprinting is a phenomenon that arises when the expression of genes depends on the parental origin of alleles. Epigenetic mechanisms may induce the full or partial suppression of maternal or paternal alleles, thereby leading to different types of imprinting. However, imprinting effects have received little consideration in animal breeding programmes, although their relevance to some agricultural important traits has been demonstrated. A recently proposed model (imprinting model) with two path-of-transmission (male and female)-specific breeding values for each animal accounts for all types of imprinting simultaneously (paternal, maternal, full and partial)...
December 6, 2016: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/27916961/recent-progress-in-electrochemical-biosensors-for-glycoproteins
#18
REVIEW
Uichi Akiba, Jun-Ichi Anzai
This review provides an overview of recent progress in the development of electrochemical biosensors for glycoproteins. Electrochemical glycoprotein sensors are constructed by combining metal and carbon electrodes with glycoprotein-selective binding elements including antibodies, lectin, phenylboronic acid and molecularly imprinted polymers. A recent trend in the preparation of glycoprotein sensors is the successful use of nanomaterials such as graphene, carbon nanotube, and metal nanoparticles. These nanomaterials are extremely useful for improving the sensitivity of glycoprotein sensors...
December 1, 2016: Sensors
https://www.readbyqxmd.com/read/27916849/one-step-fabrication-of-microchannels-with-integrated-three-dimensional-features-by-hot-intrusion-embossing
#19
Mike Debono, Dan Voicu, Mohammad Pousti, Muhammad Safdar, Robert Young, Eugenia Kumacheva, Jesse Greener
We build on the concept of hot intrusion embossing to develop a one-step fabrication method for thermoplastic microfluidic channels containing integrated three-dimensional features. This was accomplished with simple, rapid-to-fabricate imprint templates containing microcavities that locally control the intrusion of heated thermoplastic based on their cross-sectional geometries. The use of circular, rectangular and triangular cavity geometries was demonstrated for the purposes of forming posts, multi-focal length microlense arrays, walls, steps, tapered features and three-dimensional serpentine microchannels...
November 29, 2016: Sensors
https://www.readbyqxmd.com/read/27916660/isoform-switch-of-tet1-regulates-dna-demethylation-and-mouse-development
#20
Wenhao Zhang, Weikun Xia, Qiujun Wang, Aaron J Towers, Jiayu Chen, Rui Gao, Yu Zhang, Chia-An Yen, Ah Young Lee, Yuanyuan Li, Chen Zhou, Kaili Liu, Jing Zhang, Tian-Peng Gu, Xiuqi Chen, Zai Chang, Danny Leung, Shaorong Gao, Yong-Hui Jiang, Wei Xie
The methylcytosine oxidase TET proteins play important roles in DNA demethylation and development. However, it remains elusive how exactly they target substrates and execute oxidation. Interestingly, we found that, in mice, the full-length TET1 isoform (TET1e) is restricted to early embryos, embryonic stem cells (ESCs), and primordial germ cells (PGCs). By contrast, a short isoform (TET1s) is preferentially expressed in somatic cells, which lacks the N terminus including the CXXC domain, a DNA-binding module that often recognizes CpG islands (CGIs) where TET1 predominantly occupies...
November 15, 2016: Molecular Cell
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