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Vibrio cholerae

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https://www.readbyqxmd.com/read/29678912/characterizing-the-ecology-of-vibrio-in-the-neuse-river-estuary-north-carolina-using-heat-shock-protein-60-hsp60-next-generation-amplicon-sequencing
#1
Kelsey J Jesser, Rachel T Noble
Of marine eubacteria, the genus Vibrio is intriguing because member species are relevant to both marine ecology and human health. Many have touted the relationships of Vibrio to environmental factors, especially temperature and salinity, to predict total Vibrio abundance, but these studies lacked the taxonomic resolution to identify relationships among species and key drivers of Vibrio dynamics. In order to improve NGS surveys of Vibrio, we have conducted both 16S small subunit ribosomal RNA (rRNA) and heat shock protein 60 ( hsp60 ) amplicon sequencing of water samples collected at two well-studied locations in the Neuse River Estuary, NC...
April 20, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29670590/antimicrobial-potential-of-epiphytic-bacteria-associated-with-seaweeds-of-little-andaman-india
#2
Perumal Karthick, Raju Mohanraju
Seaweeds of the intertidal regions are a rich source of surface associated bacteria and are potential source of antimicrobial molecules. In the present study, 77 epiphytic isolates from eight different algae collected from Little Andaman were enumerated. On testing for their antimicrobial activities against certain pathogens twelve isolates showed positive and six of them showed significant antimicrobial inhibition zone against Shigella boydii type 1, Shigella flexneri type 2a, Shigella dysenteriae type 5, Enterotoxigenic Escherichia coli O115, Enteropathogenic E...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29667809/strand-displacement-probes-combined-with-isothermal-nucleic-acid-amplification-for-instrument-free-detection-from-complex-samples
#3
Elizabeth A Phillips, Taylor J Moehling, Sanchita Bhadra, Andrew D Ellington, Jacqueline C Linnes
Sensitive and specific detection of pathogens via nucleic acid amplification is currently constrained to laboratory settings and portable equipment with costly fluorescent detectors. Nucleic acid-detecting lateral flow immunoassay strips (LFIAs) offer a low-cost visual transduction strategy at points of need. Unfortunately, these LFIAs frequently detect amplification byproducts that can yield spurious results which can only be deciphered through statistical analysis. We integrated customizable strand displacement probes into standard loop mediated isothermal amplification (LAMP) assays to prevent byproduct capture on commercial LFIAs...
April 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29666284/biofilms-comprise-a-component-of-the-annual-cycle-of-vibrio-cholerae-in-the-bay-of-bengal-estuary
#4
Marzia Sultana, Suraia Nusrin, Nur A Hasan, Abdus Sadique, Kabir U Ahmed, Atiqul Islam, Anwar Hossain, Ira Longini, Azhar Nizam, Anwar Huq, Abul K Siddique, David A Sack, Richard B Sack, Rita R Colwell, Munirul Alam
Vibrio cholerae , an estuarine bacterium, is the causative agent of cholera, a severe diarrheal disease that demonstrates seasonal incidence in Bangladesh. In an extensive study of V. cholerae occurrence in a natural aquatic environment, water and plankton samples were collected biweekly between December 2005 and November 2006 from Mathbaria, an estuarine village of Bangladesh near the mangrove forests of the Sundarbans. Toxigenic V. cholerae exhibited two seasonal growth peaks, one in spring (March to May) and another in autumn (September to November), corresponding to the two annual seasonal outbreaks of cholera in this region...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29665368/streptomyces-thermoviolaceus-src3-strain-as-a-novel-source-of-the-antibiotic-adjuvant-streptazolin-a-statistical-approach-toward-the-optimized-production
#5
Ibtissem Djinni, Warda Djoudi, Samiha Souagui, Farida Rabia, Sihem Rahmouni, Ines Mancini, Mouloud Kecha
Streptomyces thermoviolaceus SRC3, a newly isolated actinobacterial strain from Algerian river sediments, exhibited a broad activity against various bacterial and yeast human pathogens (Salmonella typhi ATCC 14028, Vibrio cholerae ATCC 14035, MRSA ATCC 43300 and Candida albicans ATCC 10231). The strain SRC3 was selected from thirty nine actinobacterial isolates and identified as S. thermoviolaceus based on morphology, cultural properties, physiological analyses and 16S rRNA gene sequencing. Culture parameters for the antibiotic production were optimized by sequential statistical strategy including Plackett-Burman design (PBD) and Response Surface Methodology (RSM)...
April 14, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29664387/cholera-epidemic-in-south-sudan-and-uganda-and-need-for-international-collaboration-in-cholera-control
#6
Abdinasir Abubakar, Godfrey Bwire, Andrew S Azman, Malika Bouhenia, Lul L Deng, Joseph F Wamala, John Rumunu, Atek Kagirita, Jean Rauzier, Lise Grout, Stephen Martin, Christopher Garimoi Orach, Francisco J Luquero, Marie-Laure Quilici
Combining the official cholera line list data and outbreak investigation reports from the ministries of health in Uganda and South Sudan with molecular analysis of Vibrio cholerae strains revealed the interrelatedness of the epidemics in both countries in 2014. These results highlight the need for collaboration to control cross-border outbreaks.
May 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29661863/-vibrio-cholerae-outer-membrane-vesicles-inhibit-bacteriophage-infection
#7
Tamara Reyes-Robles, Rebecca S Dillard, Lynne S Cairns, Cecilia A Silva-Valenzuela, Max Housman, Afsar Ali, Elizabeth R Wright, Andrew Camilli
Novel preventatives could help in efforts to limit Vibrio cholerae infection and the spread of cholera. Bacteriophage (or phage) treatment has been proposed to be an alternative intervention, given the rapid replication of virulent phages, prey specificity, and relative ease of finding new virulent phages. Phage tropism is dictated in part by the presence of phage receptors on the bacterial surface. While many phages that can kill V. cholerae have been isolated, whether this pathogen is able to defend itself by neutralizing phage binding is unknown...
April 16, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29659916/human-gut-microbiota-predicts-susceptibility-to-vibrio-cholerae-infection
#8
Firas S Midani, Ana A Weil, Fahima Chowdhury, Yasmin A Begum, Ashraful I Khan, Meti D Debela, Heather K Durand, Aspen T Reese, Sai N Nimmagadda, Justin D Silverman, Crystal N Ellis, Edward T Ryan, Stephen B Calderwood, Jason B Harris, Firdausi Qadri, Lawrence A David, Regina C LaRocque
Background: Cholera is a public health problem worldwide and the risk factors for infection are only partially understood. Methods: We prospectively studied household contacts of cholera patients to compare those who were infected with those who were not. We constructed predictive machine learning models of susceptibility using baseline gut microbiota data. We identified bacterial taxa associated with susceptibility to Vibrio cholerae infection and tested these taxa for interactions with V...
April 12, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29659578/cell-adhesion-and-fluid-flow-jointly-initiate-genotype-spatial-distribution-in-biofilms
#9
Ricardo Martínez-García, Carey D Nadell, Raimo Hartmann, Knut Drescher, Juan A Bonachela
Biofilms are microbial collectives that occupy a diverse array of surfaces. It is well known that the function and evolution of biofilms are strongly influenced by the spatial arrangement of different strains and species within them, but how spatiotemporal distributions of different genotypes in biofilm populations originate is still underexplored. Here, we study the origins of biofilm genetic structure by combining model development, numerical simulations, and microfluidic experiments using the human pathogen Vibrio cholerae...
April 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29659574/the-impact-of-school-water-sanitation-and-hygiene-improvements-on-infectious-disease-using-serum-antibody-detection
#10
Anna N Chard, Victoria Trinies, Delynn M Moss, Howard H Chang, Seydou Doumbia, Patrick J Lammie, Matthew C Freeman
BACKGROUND: Evidence from recent studies assessing the impact of school water, sanitation and hygiene (WASH) interventions on child health has been mixed. Self-reports of disease are subject to bias, and few WASH impact evaluations employ objective health measures to assess reductions in disease and exposure to pathogens. We utilized antibody responses from dried blood spots (DBS) to measure the impact of a school WASH intervention on infectious disease among pupils in Mali. METHODOLOGY/PRINCIPAL FINDINGS: We randomly selected 21 beneficiary primary schools and their 21 matched comparison schools participating in a matched-control trial of a comprehensive school-based WASH intervention in Mali...
April 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29657131/unusual-constriction-zones-in-the-major-porins-ompu-and-ompt-from-vibrio-cholerae
#11
Monisha Pathania, Silvia Acosta-Gutierrez, Satya Pratyusha Bhamidimarri, Arnaud Baslé, Mathias Winterhalter, Matteo Ceccarelli, Bert van den Berg
The outer membranes (OM) of many Gram-negative bacteria contain general porins, which form nonspecific, large-diameter channels for the diffusional uptake of small molecules required for cell growth and function. While the porins of Enterobacteriaceae (e.g., E. coli OmpF and OmpC) have been extensively characterized structurally and biochemically, much less is known about their counterparts in Vibrionaceae. Vibrio cholerae, the causative agent of cholera, has two major porins, OmpU and OmpT, for which no structural information is available despite their importance for the bacterium...
April 3, 2018: Structure
https://www.readbyqxmd.com/read/29655627/lipopolysaccharide-specific-memory-b-cell-responses-to-an-attenuated-live-cholera-vaccine-are-associated-with-protection-against-vibrio-cholerae-infection
#12
Douglas J Haney, Michael D Lock, Marc Gurwith, Jakub K Simon, Glenn Ishioka, Mitchell B Cohen, Beth D Kirkpatrick, Caroline E Lyon, Wilbur H Chen, Marcelo B Sztein, Myron M Levine, Jason B Harris
BACKGROUND: The single-dose live attenuated vaccine CVD 103-HgR protects against experimental Vibrio cholerae infection in cholera-naïve adults for at least 6 months after vaccination. While vaccine-induced vibriocidal seroconversion is associated with protection, vibriocidal titers decline rapidly from their peak 1-2 weeks after vaccination. Although vaccine-induced memory B cells (MBCs) might mediate sustained protection in individuals without detectable circulating antibodies, it is unknown whether oral cholera vaccination induces a MBC response...
April 11, 2018: Vaccine
https://www.readbyqxmd.com/read/29649471/principal-coordinate-analysis-assisted-chromatographic-analysis-of-bacterial-cell-wall-collection-a-robust-classification-approach
#13
Keshav Kumar, Felipe Cava
In the present work, Principal coordinate analysis (PCoA) is introduced to develop a robust model to classify the chromatographic data sets of peptidoglycan sample. PcoA captures the heterogeneity present in the data sets by using the dissimilarity matrix as input. Thus, in principle, it can even capture the subtle differences in the bacterial peptidoglycan composition and can provide a more robust and fast approach for classifying the bacterial collection and identifying the novel cell wall targets for further biological and clinical studies...
April 9, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29630036/paraffin-embedding-and-thin-sectioning-of-microbial-colony-biofilms-for-microscopic-analysis
#14
William C Cornell, Chase J Morgan, Leslie Koyama, Hassan Sakhtah, Jennifer H Mansfield, Lars E P Dietrich
Sectioning via paraffin embedding is a broadly established technique in eukaryotic systems. Here we provide a method for the fixation, embedding, and sectioning of intact microbial colony biofilms using perfused paraffin wax. To adapt this method for use on colony biofilms, we developed techniques for maintaining each sample on its growth substrate and laminating it with an agar overlayer, and added lysine to the fixative solution. These optimizations improve sample retention and preservation of micromorphological features...
March 23, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29625977/spatiotemporal-dynamics-of-vibrio-cholerae-in-turbid-alkaline-lakes-determined-with-quantitative-pcr
#15
Rupert Bliem, Georg Reischer, Rita Linke, Andreas Farnleitner, Alexander Kirschner
In recent years, global warming has led to a growing number of Vibrio cholerae infections of bathing water users in regions formerly unaffected by this pathogen. It is therefore of high importance to monitor V. cholerae in the aquatic environments and to elucidate the main factors governing their prevalence and abundance. For this purpose, rapid and standardizable methods that can be performed by routine water laboratories are prerequisite. In this study we applied a recently developed multiplex quantitative PCR strategy to (i) monitor the spatiotemporal variability of V...
April 6, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29624592/anti-o-specific-polysaccharide-osp-immune-responses-following-vaccination-with-oral-cholera-vaccine-cvd-103-hgr-correlate-with-protection-against-cholera-after-infection-with-wild-type-vibrio-cholerae-o1-el-tor-inaba-in-north-american-volunteers
#16
Kamrul Islam, Motaher Hossain, Meagan Kelly, Leslie M Mayo Smith, Richelle C Charles, Taufiqur Rahman Bhuiyan, Pavol Kováč, Peng Xu, Regina C LaRocque, Stephen B Calderwood, Jakub K Simon, Wilbur H Chen, Douglas Haney, Michael Lock, Caroline E Lyon, Beth D Kirkpatrick, Mitchell Cohen, Myron M Levine, Marc Gurwith, Jason B Harris, Firdausi Qadri, Edward T Ryan
BACKGROUND: Cholera is an acute voluminous dehydrating diarrheal disease caused by toxigenic strains of Vibrio cholerae O1 and occasionally O139. A growing body of evidence indicates that immune responses targeting the O-specific polysaccharide (OSP) of V. cholerae are involved in mediating protection against cholera. We therefore assessed whether antibody responses against OSP occur after vaccination with live attenuated oral cholera vaccine CVD 103-HgR, and whether such responses correlate with protection against cholera...
April 6, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29621708/abundances-and-profiles-of-antibiotic-resistance-genes-as-well-as-co-occurrences-with-human-bacterial-pathogens-in-ship-ballast-tank-sediments-from-a-shipyard-in-jiangsu-province-china
#17
Baoyi Lv, Yuxue Cui, Wen Tian, Jing Li, Bing Xie, Fang Yin
Ship ballasting operations may transfer harmful aquatic organisms across global ocean. This study aims to reveal the occurrences and abundances of antibiotic resistance genes (ARGs) and human bacterial pathogens (HBPs) in ballast tank sediments. Nine samples were collected and respectively analyzed by real-time quantitative PCR and high-throughput sequencing technologies. Ten ARGs (aadA1, blaCTX-M, blaTEM, ermB, mefA, strB, sul1, sul2, tetM, and tetQ) and the Class-I integron gene (intI1) were highly prevalent (105 -109 gene copies/g) in ballast tank sediments...
April 2, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29621296/inhibition-of-biofilm-formation-on-the-surface-of-water-storage-containers-using-biosand-zeolite-silver-impregnated-clay-granular-and-silver-impregnated-porous-pot-filtration-systems
#18
Phumudzo Budeli, Resoketswe Charlotte Moropeng, Lizzy Mpenyana-Monyatsi, Maggie Ndombo Benteke Momba
Development of biofilms occurring on the inner surface of storage vessels offers a suitable medium for the growth of microorganisms and consequently contributes to the deterioration of treated drinking water quality in homes. The aim of this study was to determine whether the two point-of-use technologies (biosand zeolite silver-impregnated clay granular (BSZ-SICG) filter and silver-impregnated porous pot (SIPP) filter) deployed in a rural community of South Africa could inhibit the formation of biofilm on the surface of plastic-based containers generally used by rural households for the storage of their drinking water...
2018: PloS One
https://www.readbyqxmd.com/read/29616596/decoding-and-modelling-of-time-series-count-data-using-poisson-hidden-markov-model-and-markov-ordinal-logistic-regression-models
#19
Tunny Sebastian, Visalakshi Jeyaseelan, Lakshmanan Jeyaseelan, Shalini Anandan, Sebastian George, Shrikant I Bangdiwala
Hidden Markov models are stochastic models in which the observations are assumed to follow a mixture distribution, but the parameters of the components are governed by a Markov chain which is unobservable. The issues related to the estimation of Poisson-hidden Markov models in which the observations are coming from mixture of Poisson distributions and the parameters of the component Poisson distributions are governed by an m-state Markov chain with an unknown transition probability matrix are explained here...
January 1, 2018: Statistical Methods in Medical Research
https://www.readbyqxmd.com/read/29616005/a-comparative-analysis-of-vibrio-cholerae-contamination-in-point-of-drinking-and-source-water-in-a-low-income-urban-community-bangladesh
#20
Jannatul Ferdous, Rebeca Sultana, Ridwan B Rashid, Md Tasnimuzzaman, Andreas Nordland, Anowara Begum, Peter K M Jensen
Bangladesh is a cholera endemic country with a population at high risk of cholera. Toxigenic and non-toxigenic Vibrio cholerae ( V. cholerae ) can cause cholera and cholera-like diarrheal illness and outbreaks. Drinking water is one of the primary routes of cholera transmission in Bangladesh. The aim of this study was to conduct a comparative assessment of the presence of V. cholerae between point-of-drinking water and source water, and to investigate the variability of virulence profile using molecular methods of a densely populated low-income settlement of Dhaka, Bangladesh...
2018: Frontiers in Microbiology
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