keyword
MENU ▼
Read by QxMD icon Read
search

Recent studies

keyword
https://www.readbyqxmd.com/read/29224252/are-inhaled-steroids-safe-and-effective-for-prevention-or-treatment-of-bronchopulmonary-dysplasia
#1
Eric S Shinwell
Bronchopulmonary Dysplasia (BPD) represents a major threat to the short and long-term health and development of very preterm infants. Unfortunately, despite much study and progress in neonatology in recent years and some modest improvements in survival, both the incidence and severity of BPD remain frustratingly stable (1). This article is protected by copyright. All rights reserved.
December 9, 2017: Acta Paediatrica
https://www.readbyqxmd.com/read/29224215/deleterious-variants-in-dchs1-are-prevalent-in-sporadic-cases-of-mitral-valve-prolapse
#2
Alisson Clemenceau, Jean-Christophe Bérubé, Paméla Bélanger, Nathalie Gaudreault, Maxime Lamontagne, Oumhani Toubal, Marie-Annick Clavel, Romain Capoulade, Patrick Mathieu, Philippe Pibarot, Yohan Bosse
BACKGROUND: A recent study identified DCHS1 as a causal gene for mitral valve prolapse. The goal of this study is to investigate the presence and frequency of known and novel variants in this gene in 100 asymptomatic patients with moderate to severe organic mitral regurgitation. METHODS: DNA sequencing assays were developed for two previously identified functional missense variants, namely p.R2330C and p.R2513H, and all 21 exons of DCHS1. Pathogenicity of variants was evaluated in silico...
December 10, 2017: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29224194/establishment-and-evaluation-of-an-indirect-immunofluorescence-assay-for-the-detection-of-salmonid-alphavirus
#3
S Gao, W Shi, Y T Wang, M T Guo, K X Duan, A C Song, G H Lian, Tong Ren, Y J Li, L J Tang, L Sun, M Liu
Salmonid alphaviruses (SAV) severely infects farmed salmonids and rainbow trout. Owing to the recent increase in fish import trade, several SAV-unreported countries, such as China, may face serious threat of this infection. Thus, it is necessary to develop efficient detection methods for the prevention and diagnosis of SAV infection. In this study, we selected a conserved segment of the SAV E1 protein as a target immunogen for the preparation of monoclonal antibodies (mAbs) specific to SAV. A sensitive indirect immunofluorescence (IF) method was developed using 26E9C2 mAb that detected E1 protein and identified subtypes 1, 2 and 5 of SAV...
December 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29224190/metabolic-surgery-for-the-treatment-of-type-2-diabetes-in-obese-individuals
#4
REVIEW
David E Cummings, Francesco Rubino
Several bariatric operations originally designed to promote weight loss have been found to powerfully treat type 2 diabetes, causing remission in most cases, through diverse mechanisms additional to the secondary consequences of weight loss. These observations have prompted consideration of such operations as 'metabolic surgery', used expressly to treat diabetes, including among patients who are only mildly obese or merely overweight. Large, long-term observational studies consistently demonstrate that bariatric/metabolic surgery is associated with reductions in all cardiovascular risk factors, actual cardiovascular events, microvascular diabetes complications, cancer and death...
December 9, 2017: Diabetologia
https://www.readbyqxmd.com/read/29224175/thalamic-interactions-of-cerebellum-and-basal-ganglia
#5
REVIEW
Andreas Hintzen, Esther Annegret Pelzer, Marc Tittgemeyer
Cerebellum and basal ganglia are reciprocally interconnected with the neocortex via oligosynaptic loops. The signal pathways of these loops predominantly converge in motor areas of the frontal cortex and are mainly segregated on subcortical level. Recent evidence, however, indicates subcortical interaction of these systems. We have reviewed literature that addresses the question whether, and to what extent, projections of main output nuclei of basal ganglia (reticular part of the substantia nigra, internal segment of the globus pallidus) and cerebellum (deep cerebellar nuclei) interact with each other in the thalamus...
December 9, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/29224170/affinity-based-enrichment-techniques-for-the-genome-wide-analysis-of-5-hydroxymethylcytosine
#6
John P Thomson, Richard R Meehan
Since its initial characterization in 2009 there has been a great degree of interest in comparative profiling of 5-hydroxymethylcytosine (5hmC) nucleotides in vertebrate DNA. Through a host of genome-wide studies the distribution of 5hmC has been mapped in a range of cell lines, tissue types and organisms; the majority of which have been generated through affinity-based methods for 5hmC enrichment. Although recent advances in the field have resulted in the ability to investigate the levels of both methylated and hydroxymethylated cytosines at single base resolution, such studies are still relatively cost-prohibitive as well as technically challenging...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224139/antibody-based-detection-of-global-nuclear-dna-methylation-in-cells-tissue-sections-and-mammalian-embryos
#7
Nathalie Beaujean, Juliette Salvaing, Nur Annies Abd Hadi, Sari Pennings
Immunostaining is widely used in cell biology for the in situ detection of proteins in fixed cells. The method is based on the specificity of antibodies for recognizing and binding to a selected target, combined with immunolabeling techniques for microscopic imaging. Antibodies with high specificities for modified nucleotides have also been widely developed, and among those, antibodies that recognize modified cytosine: 5-methylcytosine (5mC), and more recently, its derivates 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC)...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224136/a-summary-of-the-biological-processes-disease-associated-changes-and-clinical-applications-of-dna-methylation
#8
Gitte Brinch Andersen, Jörg Tost
DNA methylation at cytosines followed by guanines, CpGs, forms one of the multiple layers of epigenetic mechanisms controlling and modulating gene expression through chromatin structure. It closely interacts with histone modifications and chromatin remodeling complexes to form the local genomic and higher-order chromatin landscape. DNA methylation is essential for proper mammalian development, crucial for imprinting and plays a role in maintaining genomic stability. DNA methylation patterns are susceptible to change in response to environmental stimuli such as diet or toxins, whereby the epigenome seems to be most vulnerable during early life...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224133/epidemiology-of-chronic-hepatitis-b-virus-in-ireland-using-routinely-collected-surveillance-and-administrative-data-2004-2014
#9
Caroline E van Gemert, Niamh Murphy, Tara A Mitchell, Margaret E Hellard, Thornton Thornton
BACKGROUND: Ireland has a low prevalence of chronic hepatitis B virus (HBV) infection; however, there are limited recently published epidemiological data. This study aimed to describe the epidemiology of chronic HBV in Ireland between 2004 and 2014 using routine surveillance data and identify and interrogate additional data sources in Ireland to complement the interpretation of HBV surveillance data. METHODS: Routinely collected passive surveillance data of notified cases of HBV infection were collated for 2004-2014...
December 9, 2017: Irish Journal of Medical Science
https://www.readbyqxmd.com/read/29224100/estrogens-and-body-weight-regulation-in-men
#10
Katya B Rubinow
Our understanding of the metabolic roles of sex steroids in men has evolved substantially over recent decades. Whereas testosterone once was believed to contribute to metabolic risk in men, the importance of adequate androgen exposure for the maintenance of metabolic health has been demonstrated unequivocally. A growing body of evidence now also supports a critical role for estrogens in metabolic regulation in men. Recent data from clinical intervention studies indicate that estradiol may be a stronger determinant of adiposity than testosterone in men, and even short-term estradiol deprivation contributes to fat mass accrual...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29224094/sexual-dimorphism-and-estrogen-action-in-mouse-liver
#11
Sara Della Torre, Federica Lolli, Paolo Ciana, Adriana Maggi
Recent studies have demonstrated that in mice, the estrogen receptor alpha (ERα) is expressed in the liver and has a direct effect on the regulation of the hepatic genes relevant for energy metabolism and drug metabolism. The sex-related differential expression of the hepatic ERα raises the questions as to whether this receptor is responsible for the sexual differences observed in the physiopathology of the liver.
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29224083/electron-transfer-between-enzymes-and-electrodes
#12
Tanja Vidakovic-Koch
Efficient electron transfer between redox enzymes and electrocatalytic surfaces plays a significant role in development of novel energy conversion devices as well as novel reactors for production of commodities and fine chemicals. Major application examples are related to enzymatic fuel cells and electroenzymatic reactors, as well as enzymatic biosensors. The two former applications are still at the level of proof-of-concept, partly due to the low efficiency and obstacles to electron transfer between enzymes and electrodes...
December 10, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/29224069/clickseq-replacing-fragmentation-and-enzymatic-ligation-with-click-chemistry-to-prevent-sequence-chimeras
#13
Elizabeth Jaworski, Andrew Routh
We recently reported a fragmentation-free method for the synthesis of Next-Generation Sequencing libraries called "ClickSeq" that uses biorthogonal click-chemistry in place of enzymes for the ligation of sequencing adaptors. We found that this approach dramatically reduces artifactual chimera formation, allowing the study of rare recombination events that include viral replication intermediates and defective-interfering viral RNAs. ClickSeq illustrates how robust, bio-orthogonal chemistry can be harnessed in vitro to capture and dissect complex biological processes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224067/multiplexed-targeted-sequencing-for-oxford-nanopore-minion-a-detailed-library-preparation-procedure
#14
Timokratis Karamitros, Gkikas Magiorkinis
MinION is a small form factor sequencer recently retailed by Oxford Nanopore technologies. This lighter-sized USB3.0-interfaced device uses innovative nanotechnology to generate extra-long reads from libraries prepared using only standard molecular biology lab equipment. The flexibility and the portability of the platform makes it ideal for point-of-interest and real-time surveillance applications. However, MinION's limited capacity is not enough for the study of specific targets within larger genomes. Apart from just PCR-amplifying regions of interest, the capture of long reads spanning the edges of known-unknown genomic regions is of great importance for structural studies, such as the identification of mobile elements' integrations sites, bridging over low complexity repetitive regions etc...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224043/identification-of-human-papillomavirus-hpv-subtype-in-oral-cancer-patients-through-microarray-technology
#15
Soung Min Kim, Ik Jae Kwon, Hoon Myoung, Jong Ho Lee, Suk Keun Lee
PURPOSE: Human papilloma virus (HPV) is the main source of cervical cancer. Many recent studies have revealed the prevalence and prognosis of HPV associated with oropharyngeal squamous cell carcinoma, but fewer reports have evaluated HPV in oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the prevalence and prognosis of HPV associated with OSCC according to HPV and tumor types. MATERIALS AND METHODS: We used a DNA chip kit (MY-HPV chip kit ®, Mygene Co...
December 9, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29224025/alterations-in-adiposity-and-glucose-homeostasis-in-adult-gasp-1-overexpressing-mice
#16
Luce Périè, Alexis Parenté, Fabienne Baraige, Laetitia Magnol, Véronique Blanquet
BACKGROUND/AIMS: Myostatin is known as a powerful negative regulator of muscle growth playing a key role in skeletal muscle homeostasis. Recent studies revealed that myostatin-deficient mice lead to an increase of insulin sensitivity, a decrease of adiposity and a resistance to obesity, showing that myostatin can also impact on metabolism. Thus, myostatin appeared as a potential therapeutic target to treat insulin resistance. METHODS: We generated transgenic mice overexpressing Gasp-1, a myostatin inhibitor...
December 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29223967/questionnaire-based-algorithm-for-assessing-occupational-noise-exposure-of-construction-workers
#17
Kate Lewkowski, Kahlia McCausland, Jane S Heyworth, Ian W Li, Warwick Williams, Lin Fritschi
OBJECTIVES: Occupational noise exposure is a major cause of hearing loss worldwide. In order to inform preventative strategies, we need to further understand at a population level which workers are most at risk. METHODS: We have developed a new questionnaire-based algorithm that evaluates an individual worker's noise exposure. The questionnaire and supporting algorithms are embedded into the existing software platform, OccIDEAS. Based on the tasks performed by a worker during their most recent working shift and using a library of task-based noise exposure levels, OccIDEAS estimates whether a worker has exceeded the full-shift workplace noise exposure limit (LAeq,8h≥85 dBA)...
December 9, 2017: Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/29223963/field-evaluation-of-two-point-of-care-tests-for-syphilis-among-men-who-have-sex-with-men-verona-italy
#18
Antonella Zorzi, Maddalena Cordioli, Lorenzo Gios, Paola Del Bravo, Igor Toskin, Rosanna W Peeling, Karel Blondeel, Giuseppe Cornaglia, James Kiarie, Ronald Ballard, Massimo Mirandola
OBJECTIVES: The incidence of HIV and syphilis among men who have sex with men (MSM) in Europe has recently increased. Rapid point-of-care tests (POCTs) for syphilis can improve access to screening. The purpose of this study was to evaluate the performance of two syphilis POCTs compared with laboratory tests among MSM. METHODS: The study was undertaken in Verona, Italy. Asymptomatic MSM, potentially exposed to syphilis, were enrolled prospectively. The POCTs evaluated were SD Bioline Syphilis 3...
December 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29223961/a-systematic-review-and-meta-analysis-of-studies-evaluating-the-performance-of-point-of-care-tests-for-human-papillomavirus-screening
#19
REVIEW
Helen Kelly, Philippe Mayaud, Michel Segondy, Nitika Pant Pai, Rosanna W Peeling
BACKGROUND: High-risk human papillomavirus (HPV) is a necessary cause of high-grade cervical intraepithelial neoplasia (grade 2 or higher, CIN2+). Simplified and rapid HPV DNA assays designed for use in resource-limited settings have recently become available. METHODS: We performed a systematic review and meta-analysis by searching Medline, Embase, Global Health and CINAHL databases for studies from 1 January 2004 to 25 February 2017 that reported the performance of careHPV or OncoE6 for the detection of histological CIN2+ in cervical cancer screening...
December 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/29223951/heart-rate-variability-in-bipolar-disorder-and-borderline-personality-disorder-a-clinical-review
#20
REVIEW
Oliver Carr, Maarten de Vos, Kate E A Saunders
Heart rate variability (HRV) in psychiatric disorders has become an increasing area of interest in recent years following technological advances that enable non-invasive monitoring of autonomic nervous system regulation. However, the clinical interpretation of HRV features remain widely debated or unknown. Standardisation within studies of HRV in psychiatric disorders is poor, making it difficult to reproduce or build on previous work. Recently, a Guidelines for Reporting Articles on Psychiatry and Heart rate variability checklist has been proposed to address this issue...
December 9, 2017: Evidence-based Mental Health
keyword
keyword
4763
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"