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https://www.readbyqxmd.com/read/28446778/tumor-necrosis-factor-prevents-candida-albicans-biofilm-formation
#1
Francisco Airton Castro Rocha, Anelise Maria Costa Vasconcelos Alves, Marcos Fábio Gadelha Rocha, Rossana de Aguiar Cordeiro, Raimunda Sâmia Nogueira Brilhante, Ana Carolina Matias Dinelly Pinto, Rodolfo de Melo Nunes, Virgínia Cláudia Carneiro Girão, José Julio Costa Sidrim
Candida species are commensals but some develop biofilms in prosthetic materials and host surfaces that may represent up to 30% of deaths related to infections, particularly in immunosuppressed patients. Tumor necrosis factor (TNF) exhibits a plethora of functions in host defense mechanisms whereas excessive release of TNF in inflammation promotes tissue damage. Cytokines released in an inflammatory milieu may influence the development of microorganisms either by promoting their growth or displaying antimicrobial activity...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28441606/growth-inhibition-and-antibiofilm-potential-of-ag-nanoparticles-coated-with-lectin-an-arthropod-immune-molecule
#2
Sangily Jayanthi, Sathappan Shanthi, Baskaralingam Vaseeharan, Narayanan Gopi, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
Lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. Here, lectin was purified from the haemolymph of blue swimmer crab Portunus pelagicus, using mannose-coupled sepharose CL-4B affinity column chromatography. In SDS-PAGE analysis, lectin showed a molecular mass of approximately 155kDa. The synthesis of lectin-coated silver nanoparticles (lectin-coated AgNPs) was confirmed by UV-Vis spectroscopy, XRD, FTIR, SEM, TEM, SAED, and EDX analysis...
April 13, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28439794/the-effectiveness-of-voriconazole-in-therapy-of-candida-glabrata-s-biofilms-oral-infections-and-its-influence-on-the-matrix-composition-and-gene-expression
#3
Célia F Rodrigues, Bruna Gonçalves, Maria Elisa Rodrigues, Sónia Silva, Joana Azeredo, Mariana Henriques
Candida glabrata is one of most prevalent yeast in fungal infections, especially in immunocompromised patients. Its azole resistance results in a low therapeutic response, particularly when associated with biofilms. The main goal of this work was to study the effectiveness of voriconazole (Vcz) against C. glabrata biofilms oral pathologies, as esophageal or oropharyngeal candidiasis. Antifungal susceptibilities were determined in pre-formed 24-h-biofilms and ERG genes expression was determined by qRT-PCR. Protein quantification was performed using BCA(®) Kit, carbohydrate was estimated according to the Dubois assay and β-1,3 glucans concentration were determined using Glucatell(®) kit...
April 24, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28439502/ambroxol-hydrochloride-combined-with-fluconazole-reverses-the-resistance-of-candida-albicans-to-fluconazole
#4
Xiuyun Li, Yuanhao Zhao, Xin Huang, Cuixiang Yu, Yilei Yang, Shujuan Sun
In this study, we found that ambroxol hydrochloride (128 μg/mL) exhibits synergistic antifungal effects in combination with fluconazole (2 μg/mL) against resistant planktonic Candida albicans (C. albicans) cells. This combination also exhibited synergistic effects against resistant C. albicans biofilms in different stages (4, 8, and 12 h) according to the microdilution method. In vitro data were further confirmed by the success of this combination in treating Galleria mellonella infected by resistant C. albicans...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28438935/candida-guilliermondii-complex-is-characterized-by-high-antifungal-resistance-but-low-mortality-a-report-on-22-cases-of-candidemia
#5
Laura Judith Marcos-Zambrano, Mireia Puig-Asensio, Felipe Pérez-García, Pilar Escribano, Carlos Sánchez-Carrillo, Oscar Zaragoza, Belén Padilla, Manuel Cuenca-Estrella, Benito Almirante, M Teresa Martin-Gómez, Patricia Muñoz, Emilio Bouza, Jesús Guinea
Objectives: The objectives of our study were to describe the characteristics of patients infected with C. guilliermondii candidemia and to perform an in-depth microbiological characterization of isolates and compare them with those of patients with C. albicans candidemia.Methods: We described the risk factors and outcome of 22 patients with candidemia caused by C. guilliermondii complex. Incident isolates were identified using molecular techniques, and susceptibility to fluconazole, anidulafungin and micafungin was studied...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28434823/influence-of-serum-and-polystyrene-plate-type-on-stability-of-candida-albicans-biofilms
#6
Purity N Kipanga, Walter Luyten
Adhesion is a crucial initial step in microbial biofilm formation. Firm attachment to a target surface subsequently ensures successful colonization and survival despite turbulent conditions. In the laboratory, polystyrene plates are commonly used in biofilm experiments and the 'washing/rinse steps' before staining are critical for assaying biofilm viability. However, these rinse steps risk the removal (partially or entirely) of the formed biofilm, resulting in inconsistent results. The aim of the present study was to optimize conditions for firmer biofilms, less prone to disruption and thus significantly reducing well-to-well variability...
April 20, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28434686/effects-of-trimethylsilane-plasma-coating-on-the-hydrophobicity-of-denture-base-resin-and-adhesion-of-candida-albicans-on-resin-surfaces
#7
Tianshuang Liu, Changqi Xu, Liang Hong, Franklin Garcia-Godoy, Timothy Hottel, Jegdish Babu, Qingsong Yu
STATEMENT OF PROBLEM: Candida-associated denture stomatitis is the most common oral mucosal lesion among denture wearers. Trimethylsilane (TMS) plasma coating may inhibit the growth of Candida albicans on denture surfaces. PURPOSE: The purpose of this in vitro study was to investigate whether TMS plasma coatings can effectively reduce C albicans adhesion on denture base acrylic resin surfaces. MATERIAL AND METHODS: Sixty denture base acrylic resin disks with smooth and rough surfaces were prepared and were either left untreated (control group) or coated with TMS monomer (experimental group) by using plasma...
April 20, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28428132/novel-properties-of-hippophae-rhamnoides-l-twig-and-leaf-extracts-anti-virulence-action-and-synergy-with-antifungals-studied-in%C3%A2-vitro-on-candida-spp-model
#8
Beata Sadowska, Aleksandra Budzyńska, Anna Stochmal, Jerzy Żuchowski, Barbara Różalska
Original, chemically characterized Sea buckthorn (SBT) twig and leaf extracts were in vitro studied in terms of anti-Candida activity. Minimum inhibitory concentrations (MICs) of the extracts against C. albicans ATCC 10231 ranged: 250 μg/ml (twig), 31.5 μg/ml (leaf), and against C. glabrata G1 (clinical isolate) - 15.6 μg/ml (twig), 3.9 μg/ml (leaf). Next the extracts have been used at their subMIC. Both extracts significantly enhanced activity of fluconazole (FLC) and caspofungin (CAS) against C...
April 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28426292/efficacy-of-three-different-lasers-on-eradication-of-enterococcus-faecalis-and-candida-albicans-biofilms-in-root-canal-system
#9
Silvija Kasić, Marita Knezović, Nataša Beader, Dragana Gabrić, Ana Ivanišević Malčić, Anja Baraba
OBJECTIVE: The objective was to compare the efficacy of three different lasers in disinfection of root canals inoculated with Enterococcus faecalis and Candida albicans biofilms. BACKGROUND: Endodontic space disinfection depends on the type of root canal irrigant used and the way it is delivered and agitated because irrigants have limited ability to reach all parts of root canal system. MATERIALS AND METHODS: Thirty single-rooted human teeth were selected...
April 20, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28424663/antimicrobial-photodynamic-therapy-against-endodontic-enterococcus-faecalis-and-candida-albicans-mono-and-mixed-biofilms-in-the-presence-of-photosensitizers-a-comparative-study-with-classical-endodontic-irrigants
#10
Patrícia Diogo, Chantal Fernandes, Francisco Caramelo, Marta Mota, Isabel M Miranda, M A F Faustino, M G P M S Neves, Marciana P Uliana, Kleber T de Oliveira, João M Santos, Teresa Gonçalves
Endodontic biofilms eradication from the infected root canal system remains as the primary focus in endodontic field. In this study, it was assessed the efficacy of antimicrobial Photodynamic Therapy (aPDT) with the Zn(II)chlorin e6 methyl ester (Zn(II)e6Me) activated by red light against monospecies and mixed biofilms of Enterococcus faecalis and Candida albicans. The results were compared with the ones obtained with Rose Bengal (RB), Toluidine Blue-O (TBO), the synthetic tetracationic porphyrin (TMPyP) as well as classical endodontic irrigants (3% NaOCl, 17% EDTA and 2% CHX)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28422057/antifungal-and-anti-biofilm-activities-of-acetone-lichen-extracts-against-candida-albicans
#11
Marion Millot, Marion Girardot, Lucile Dutreix, Lengo Mambu, Christine Imbert
Candida albicans is a commensal coloniser of the human gastrointestinal tract and an opportunistic pathogen, especially thanks to its capacity to form biofilms. This lifestyle is frequently involved in infections and increases the yeast resistance to antimicrobials and immune defenses. In this context, 38 lichen acetone extracts have been prepared and evaluated for their activity against C. albicans planktonic and sessile cells. Minimum inhibitory concentrations of extracts (MICs) were determined using the broth microdilution method...
April 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28420830/antimicrobial-and-antibiofilm-activities-of-mta-supplemented-with-bismuth-lipophilic-nanoparticles
#12
Rene Hernandez-Delgadillo, Casiano Del Angel-Mosqueda, Juan Manuel Solís-Soto, Silvia Munguia-Moreno, Nayely Pineda-Aguilar, Rosa Isela Sánchez-Nájera, Shankararaman Chellam, Claudio Cabral-Romero
The objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (MTA) supplemented with bismuth lipophilic nanoparticles (BisBAL NPs). The antimicrobial activity of the composite MTA-BisBAL NPs was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. The cytotoxicity of MTA-BisBAL NPs was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. MTA-BisBAL NPs inhibited the growth of Enterococcus faecalis, Escherichia coli, and Candida albicans and also detached the biofilm of fluorescent E...
April 18, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28417912/electrospun-fiber-pads-of-cellulose-acetate-and-essential-oils-with-antimicrobial-activity
#13
Ioannis L Liakos, Alina Maria Holban, Riccardo Carzino, Simone Lauciello, Alexandru Mihai Grumezescu
The method of electrospinning was used to create nanofibers made of cellulose acetate (CA) and essential oils (EOs). CA polymer at 15% w/v was dissolved in acetone and then 1% or 5% v/v of EOs was added to the polymer solution. The utilized essential oils were rosemary and oregano oils. Then, the CA/EOs in acetone solution were electrospun, creating micro/nanofibers, approximately 700-1500 nm in diameter. Raman spectroscopy was used to detect the attachment of the EOs in the CA electrospun fibers (ESFs). Scanning electron microscopy was used to study the morphology, topography and dimensions of the ESFs...
April 12, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28417524/biofilms-of-candida-spp-from-the-ocular-conjunctiva-of-horses-with-reduced-azole-susceptibility-a-complicating-factor-for-the-treatment-of-keratomycosis
#14
Raimunda Sâmia Nogueira Brilhante, Paula Vago Bittencourt, Débora de Souza Collares Castelo-Branco, Glaucia Morgana de Melo Guedes, Jonathas Sales de Oliveira, Lucas Pereira Alencar, Rossana de Aguiar Cordeiro, Mariana Pinheiro, Evilázio Fernandes Nogueira-Filho, Waldemiro de Aquino Pereira-Neto, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
OBJECTIVES: This study aimed to assess the biofilm-forming ability of Candida spp. from the ocular conjunctiva of horses and to investigate the antifungal susceptibility of these biofilms. PROCEDURES: Initially, the biofilm-forming ability of 15 strains was assessed by crystal violet staining, which reveals the fungal biomass adhered to the polystyrene plates, and scanning electron microscopy. Then, the minimum inhibitory concentrations (MICs) of amphotericin B, fluconazole, itraconazole, and caspofungin were initially determined against strains in planktonic form...
April 18, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28411060/in%C3%A2-vitro-anti-candida-activity-of-selective-serotonin-reuptake-inhibitors-against-fluconazole-resistant-strains-and-their-activity-against-biofilm-forming-isolates
#15
Rose Anny Costa Silva, Cecília Rocha da Silva, João Batista de Andrade Neto, Anderson Ramos da Silva, Rosana Sousa Campos, Letícia Serpa Sampaio, Francisca Bruna Stefany Aires do Nascimento, Brenda da Silva Gaspar, Said Gonçalves da Cruz Fonseca, Maria Aparecida Alexandre Josino, Thalles Barbosa Grangeiro, Danielle Macedo Gaspar, David Freitas de Lucena, Manoel Odorico de Moraes, Bruno Coêlho Cavalcanti, Hélio Vitoriano Nobre Júnior
Recent research has shown broad antifungal activity of the classic antidepressants selective serotonin reuptake inhibitors (SSRIs). This fact, combined with the increased cross-resistance frequency of the genre Candida regarding the main treatment today, fluconazole, requires the development of novel therapeutic strategies. In that context, this study aimed to assess the antifungal potential of fluoxetine, sertraline, and paroxetine against fluconazole-resistant Candida spp. planktonic cells, as well as to assess the mechanism of action and the viability of biofilms treated with fluoxetine...
April 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28410367/nitric-oxide-charged-catheters-as-a-potential-strategy-for-prevention-of-hospital-acquired-infections
#16
David Margel, Mark Mizrahi, Gili Regev-Shoshani, Mary Ko, Maya Moshe, Rachel Ozalvo, Liat Shavit-Grievink, Jack Baniel, Daniel Kedar, Ofer Yossepowitch, David Lifshitz, Andrei Nadu, David Greenberg, Yossef Av-Gay
BACKGROUND: Catheter-Associated Hospital-Acquired Infections (HAI's) are caused by biofilm-forming bacteria. Using a novel approach, we generated anti-infective barrier on catheters by charging them with Nitric Oxide (NO), a naturally-produced gas molecule. NO is slowly released from the catheter upon contact with physiological fluids, and prevents bacterial colonization and biofilm formation onto catheter surfaces. AIMS AND METHODS: The aim of the study was to assess the anti-infective properties of NO-charged catheters exposed to low concentration (up to 103 CFU/ml) of microbial cells in-vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28409362/the-antibacterial-agent-moxifloxacin-inhibits-virulence-factors-of-candida-albicans-through-multitargeting
#17
Ashwini Jadhav, Bhagyashree Bansode, Datta Phule, Amruta Shelar, Rajendra Patil, Wasudev Gade, Kiran Kharat, Sankunny Mohan Karuppayil
Fluoroquinolines are broad spectrum fourth generation antibiotics. Some of the Fluoroquinolines exhibit antifungal activity. We are reporting the potential mechanism of action of a fluoroquinoline antibiotic, moxifloxacin on the growth, morphogenesis and biofilm formation of the human pathogen Candida albicans. Moxifloxacin was found to be Candidacidal in nature. Moxifloxacin seems to inhibit the yeast to Hyphal morphogenesis by affecting signaling pathways. It arrested the cell cycle of C. albicans at S phase...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28408902/the-naturally-occurring-host-defense-peptide-ll-37-and-its-truncated-mimetics-ke-18-and-kr-12-have-selected-biocidal-and-antibiofilm-activities-against-candida-albicans-staphylococcus-aureus-and-escherichia-coli-in-vitro
#18
Yu Luo, Denise T F McLean, Gerard J Linden, Danny F McAuley, Ronan McMullan, Fionnuala T Lundy
Amongst the recognized classes of naturally occurring antimicrobials, human host defense peptides are an important group with an advantage (given their source) that they should be readily translatable to medicinal products. It is also plausible that truncated versions will display some of the biological activities of the parent peptide, with the benefit that they are less costly to synthesize using solid-phase chemistry. The host defense peptide, LL-37, and two truncated mimetics, KE-18 and KR-12, were tested for their inhibitory effects and antibiofilm properties against Candida albicans, Staphylococcus aureus, and Escherichia coli, microorganisms commonly implicated in biofilm-related infections such as ventilator-associated pneumonia (VAP)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28400000/influence-of-growth-conditions-on-adhesion-of-yeast-candida-spp-and-pichia-spp-to-stainless-steel-surfaces
#19
Ružica Tomičić, Peter Raspor
An understanding of adhesion behavior of Candida and Pichia yeast under different environmental conditions is key to the development of effective preventive measures against biofilm-associated infection. Hence in this study we investigated the impact of growth medium and temperature on Candida and Pichia adherence using stainless steel (AISI 304) discs with different degrees of surface roughness (Ra = 25.20-961.9 nm), material typical for the food processing industry as well as medical devices. The adhesion of the yeast strains to stainless steel surfaces grown in Malt Extract broth (MEB) or YPD broth at three temperatures (7 °C, 37 °C, 43 °C for Candida strains and 7 °C, 27 °C, 32 °C for Pichia strains) was assessed by crystal violet staining...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28396417/enterococcus-faecalis-bacteriocin-entv-inhibits-hyphal-morphogenesis-biofilm-formation-and-virulence-of-candida-albicans
#20
Carrie E Graham, Melissa R Cruz, Danielle A Garsin, Michael C Lorenz
Enterococcus faecalis, a Gram-positive bacterium, and Candida albicans, a fungus, occupy overlapping niches as ubiquitous constituents of the gastrointestinal and oral microbiome. Both species also are among the most important and problematic, opportunistic nosocomial pathogens. Surprisingly, these two species antagonize each other's virulence in both nematode infection and in vitro biofilm models. We report here the identification of the E. faecalis bacteriocin, EntV, produced from the entV (ef1097) locus, as both necessary and sufficient for the reduction of C...
April 10, 2017: Proceedings of the National Academy of Sciences of the United States of America
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