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https://www.readbyqxmd.com/read/28652732/preparation-characterization-and-evaluation-of-amphotericin-b-loaded-mpeg-pcl-g-pei-micelles-for-local-treatment-of-oral-candida-albicans
#1
Li Zhou, Peipei Zhang, Zhuo Chen, Shaona Cai, Ting Jing, Huihui Fan, Fei Mo, Jiye Zhang, Rong Lin
Fatal Candida albicans infections in the mucosal system can occur in association with immune-compromised diseases and dysbacteriosis. Currently, amphotericin B (AmB) is considered to be the most effective antibiotic in the treatment of C. albicans infections, but its clinical application is limited by side effects and poor bioavailability. In order to use AmB in the local treatment of oral C. albicans infections, AmB/MPEG-PCL-g-PEI (monomethoxy poly(ethylene glycol)-poly(epsilon-caprolactone)-graft-polyethylenimine, MPP) micelles were prepared...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28649561/psd1-effects-on-candida-albicans-planktonic-cells-and-biofilms
#2
Sónia Gonçalves, Patrícia M Silva, Mário R Felício, Luciano N de Medeiros, Eleonora Kurtenbach, Nuno C Santos
Candida albicans is an important human pathogen, causing opportunistic infections. The adhesion of planktonic cells to a substrate is the first step for biofilm development. The antimicrobial peptide (AMP) Psd1 is a defensin isolated from Pisum sativum seeds. We tested the effects of this AMP on C. albicans biofilms and planktonic cells, comparing its activity with amphotericin B and fluconazole. Three C. albicans variants were studied, one of them a mutant deficient in glucosylceramide synthase, conferring resistance to Psd1 antifungal action...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28642749/rna-seq-reveals-enhanced-sugar-metabolism-in-streptococcus-mutans-co-cultured-with-candida-albicans-within-mixed-species-biofilms
#3
Jinzhi He, Dongyeop Kim, Xuedong Zhou, Sang-Joon Ahn, Robert A Burne, Vincent P Richards, Hyun Koo
Early childhood caries (ECC), which can lead to rampant tooth-decay that is painful and costly to treat, is one of the most prevalent infectious diseases affecting children worldwide. Previous studies support that interactions between Streptococcus mutans and Candida albicans are associated with the pathogenesis of ECC. The presence of Candida enhances S. mutans growth, fitness and accumulation within biofilms in vitro, although the molecular basis for these behaviors is undefined. Using an established co-cultivation biofilm model and RNA-Seq, we investigated how C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28636236/assessment-of-silibinin-as-a-potential-antifungal-agent-and-investigation-of-its-mechanism-of-action
#4
Dae Gyu Yun, Dong Gun Lee
Silibinin, which is derived from Silybum marianum (milk thistle), has used as a traditional remedy for liver or biliary disorders and known to have superior antioxidant activity. In addition, silibinin was recently reported to have antifungal effect related to fungal apoptosis against Candida albicans and the interest in the therapeutic effect is increasing. In this study, we found another mode of antifungal action of silibinin and its antibiofilm activity on C. albicans. To investigate influence on fungal plasma membrane, propidium iodide and bis-(1, 3-dibutylbarbituric acid) trimethineoxonol [DiBAC4 (3)] assay were primarily carried out...
June 21, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28622460/antifungal-activity-of-tyrosol-and-farnesol-used-in-combination-against-candida-species-in-the-planktonic-state-or-forming-biofilms
#5
Douglas Roberto Monteiro, Laís Salomão Arias, Renan Aparecido Fernandes, Lucas Fernando Deszo da Silva, Manoel Olavo Valentim Fernandes de Castilho, Thaís Oliveira da Rosa, Ana Paula Miranda Vieira, Fabiana Gouveia Straioto, Débora Barros Barbosa, Alberto Carlos Botazzo Delbem
AIM: This study examined the antifungal activity of the combination of tyrosol and farnesol against Candida albicans and Candida glabrata in the planktonic state or forming biofilms. METHODS AND RESULTS: The effect of drug association against Candida planktonic cells was assessed by the fractional inhibitory concentration index. Mono and dual-species biofilms were developed (24 h) and the compounds, applied during 3 days, with 2 daily treatments of 1 minute each...
June 16, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28617874/candida-albicans-mannans-mediate-streptococcus-mutans-exoenzyme-gtfb-binding-to-modulate-cross-kingdom-biofilm-development-in-vivo
#6
Geelsu Hwang, Yuan Liu, Dongyeop Kim, Yong Li, Damian J Krysan, Hyun Koo
Candida albicans is frequently detected with heavy infection by Streptococcus mutans in plaque-biofilms from children with early-childhood caries (ECC). This cross-kingdom biofilm contains an extensive matrix of extracellular α-glucans that is produced by an exoenzyme (GtfB) secreted by S. mutans. Here, we report that mannans located on the outer surface of C. albicans cell-wall mediates GtfB binding, enhancing glucan-matrix production and modulating bacterial-fungal association within biofilms formed in vivo...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28609183/farnesol-signalling-in-candida-albicans-more-than-just-communication
#7
Melanie Polke, Ines Leonhardt, Oliver Kurzai, Ilse D Jacobsen
Candida albicans is a successful colonizer of the human host, which can, under certain circumstances cause a range of clinically diverse infections. Important virulence-associated traits of the fungus, such as the dimorphic switch and biofilm formation, are controlled by the quorum sensing molecule farnesol. Given the potential of farnesol as a novel antifungal drug, there has been increasing research into the mechanism underlying farnesol sensing and action in C. albicans. However, despite the identification of various factors involved in farnesol signalling, its exact mode of action remains largely unclear...
June 13, 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28607012/potent-in-vitro-synergism-of-fluconazole-and-osthole-against-fluconazole-resistant-candida-albicans
#8
De-Dong Li, Dong Chai, Xiao-Wen Huang, Shao-Xing Guan, Jiang Du, Hao-Yu Zhang, Yan Sun, Yuan-Ying Jiang
Osthole is a natural coumarin that exhibits wide biological and pharmacological activities such as neuroprotective, osteogenic, immunomodulation, anti-tumor and anti-inflammatory effects. In this study, we investigated the antifungal effects of osthole in vitro Checkerboard microdilution assay showed that osthole has significant synergistic effect with fluconazole against fluconazole-resistant Candida albicans Similar results was obtained from growth curve assay. Meanwhile, XTT reduction assay demonstrated the synergism of fluconazole and osthole against C...
June 12, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28605597/bacterial-gtfb-augments-candida-albicans-accumulation-in-cross-kingdom-biofilms
#9
K Ellepola, Y Liu, T Cao, H Koo, C J Seneviratne
Streptococcus mutans is a biofilm-forming oral pathogen commonly associated with dental caries. Clinical studies have shown that S. mutans is often detected with Candida albicans in early childhood caries. Although the C. albicans presence has been shown to enhance bacterial accumulation in biofilms, the influence of S. mutans on fungal biology in this mixed-species relationship remains largely uncharacterized. Therefore, we aimed to investigate how the presence of S. mutans influences C. albicans biofilm development and coexistence...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28605112/candida-albicans-biofilms-comparative-analysis-of-room-temperature-and-cryofixation-for-scanning-electron-microscopy
#10
T Vila, B B Fonseca, M M L DA Cunha, G R C Dos Santos, K Ishida, E Barreto-Bergter, W DE Souza, S Rozental
Biofilms are frequently related to invasive fungal infections and are reported to be more resistant to antifungal drugs than planktonic cells. The structural complexity of the biofilm as well as the presence of a polymeric extracellular matrix (ECM) is thought to be associated with this resistant behavior. Scanning electron microscopy (SEM) after room temperature glutaraldehyde-based fixation, have been used to study fungal biofilm structure and drug susceptibility but they usually fail to preserve the ECM and, therefore, are not an optimised methodology to understand the complexity of the fungal biofilm...
June 12, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28601403/-activity-of-amphotericin-b-and-anidulafungin-alone-and-combined-against-candida-tropicalis-biofilms-developed-on-teflon-%C3%A2-and-titanium
#11
Marcelo Ernesto Fernández-Rivero, José L Del Pozo, Amparo Valentín, Victoria Fornes, Araceli Molina de Diego, Javier Pemán, Emilia Cantón
BACKGROUND: Current therapeutic strategies have a limited efficacy against Candida biofilms that form on the surfaces of biomedical devices. Few studies have evaluated the activity of antifungal agents against Candida tropicalis biofilms. OBJECTIVES: To evaluate the activity of amphotericin B (AMB) and anidulafungin (AND), alone and in combination, against C. tropicalis biofilms developed on polytetrafluoroethylene (teflon -PTFE) and titanium surfaces using time-kill assays...
June 14, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28598524/nicotine-upregulates-coaggregation-of-candida-albicans-and-streptococcus-mutans
#12
Ali Ashkanane, Grace F Gomez, John Levon, L Jack Windsor, George J Eckert, Richard L Gregory
PURPOSE: Denture stomatitis is a condition of painless inflammation of denture-bearing mucosa. Reports indicate that nicotine, the major psychoactive ingredient in tobacco, increases growth of Streptococcus mutans and Candida albicans in denture biofilm. The purpose of this study was to determine the in vitro effects of nicotine on coaggregation of C. albicans with S. mutans. MATERIAL AND METHODS: C. albicans strain ATCC 10231, S. mutans strain UA159 (ATTC 700610), and nicotine dilutions (ranging from 0 to 32 mg/ml) were used for this study...
June 9, 2017: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
https://www.readbyqxmd.com/read/28591237/association-between-pathogens-from-tracheal-aspirate-and-oral-biofilm-of-patients-on-mechanical-ventilation
#13
Luana Carneiro Diniz Souza, Vanise Barros Rodrigues da Mota, Alícia Valéria Dos Santos Zaranza de Carvalho, Rita da Graça Carvalhal Frazão Corrêa, Silvana Amado Libério, Fernanda Ferreira Lopes
The aim of this study was to detect possible associations between respiratory pathogens from tracheal aspirate and oral biofilm samples in intubated patients in an intensive care unit (ICU), and to identify the most common respiratory pathogens in oral biofilm, particularly in patients that developed ventilator-associated pneumonia (VAP). Two oral biofilm samples were collected from the tongue of intubated patients (at admission and after 48 hours) and analyzed by culture with the Antibiotic Sensitivity Test...
June 5, 2017: Brazilian Oral Research
https://www.readbyqxmd.com/read/28584159/in-vitro-antibiofilm-activity-of-eucarobustol-e-against-candida-albicans
#14
Rui-Huan Liu, Zhi-Chun Shang, Tian-Xiao Li, Ming-Hua Yang, Ling-Yi Kong
Formyl-phloroglucinol meroterpenoids (FPMs) are an important type of natural products with various bioactivities. Our antifungal susceptibility assay showed that one of the Eucalyptus robusta derived FPMs-Eucarobustol E (EE) exerted strong inhibitory effect against Candida albicans biofilms at a concentration of 16 μg/ml. EE was found to block the yeast-to-hypha transition and reduce the cellular surface hydrophobicity of the biofilm cells. RNA sequencing and real time reverse-transcription PCR analysis showed that exposure to 16 μg/ml of EE resulted in marked reduction of expressions of genes involved in hyphal growth (EFG1, CPH1, TEC1, EED1, UME6, and HGC1) and cell surface proteins (ALS3, HWP1, and SAP5)...
June 5, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28578870/the-essential-oil-of-allium-sativum-as-an-alternative-agent-against-candida-isolated-from-dental-prostheses
#15
Alejandro Mendoza-Juache, Saray Aranda-Romo, Josué R Bermeo-Escalona, Araceli Gómez-Hernández, Amaury Pozos-Guillén, Luis Octavio Sánchez-Vargas
BACKGROUND: The colonization of the surfaces of dental prostheses by Candida albicans is associated with the development of denture stomatitis. In this context, the use of fluconazole has been proposed, but its disadvantage is microbial resistance. Meanwhile, the oil of Allium sativum has shown an effect in controlling biofilm formation by C. albicans. AIMS: The objective of this study was to determine the antifungal activities of the essential oil of A. sativum and fluconazole against clinical isolates of Candida species obtained from rigid, acrylic-based partial or total dentures and to compare these agents' effects on both biofilm and planktonic cells...
June 1, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28577425/denaturing-gradient-gel-electrophoresis-profiles-of-bacteria-from-the-saliva-of-twenty-four-different-individuals-form-clusters-that-showed-no-relationship-to-the-yeasts-present
#16
Manjula M Weerasekera, Chris H Sissons, Lisa Wong, Sally A Anderson, Ann R Holmes, Richard D Cannon
OBJECTIVES: The aim was to investigate the relationship between groups of bacteria identified by cluster analysis of the DGGE fingerprints and the amounts and diversity of yeast present. METHODS: Bacterial and yeast populations in saliva samples from 24 adults were analysed using denaturing gradient gel electrophoresis (DGGE) of the bacteria present and by yeast culture. RESULTS: Eubacterial DGGE banding patterns showed considerable variation between individuals...
May 22, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28570675/mitochondrial-complex-i-bridges-a-connection-between-regulation-of-carbon-flexibility-and-gastrointestinal-commensalism-in-the-human-fungal-pathogen-candida-albicans
#17
Xinhua Huang, Xiaoqing Chen, Yongmin He, Xiaoyu Yu, Shanshan Li, Ning Gao, Lida Niu, Yinhe Mao, Yuanyuan Wang, Xianwei Wu, Wenjuan Wu, Jianhua Wu, Dongsheng Zhou, Xiangjiang Zhan, Changbin Chen
Efficient assimilation of alternative carbon sources in glucose-limited host niches is critical for colonization of Candida albicans, a commensal yeast that frequently causes opportunistic infection in human. C. albicans evolved mechanistically to regulate alternative carbon assimilation for the promotion of fungal growth and commensalism in mammalian hosts. However, this highly adaptive mechanism that C. albicans employs to cope with alternative carbon assimilation has yet to be clearly understood. Here we identified a novel role of C...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28560581/antimicrobial-and-anti-biofilm-properties-of-polypropylene-meshes-coated-with-metal-containing-dlc-thin-films
#18
Elisa M Cazalini, Walter Miyakawa, Guilherme R Teodoro, Argemiro S S Sobrinho, José E Matieli, Marcos Massi, Cristiane Y Koga-Ito
A promising strategy to reduce nosocomial infections related to prosthetic meshes is the prevention of microbial colonization. To this aim, prosthetic meshes coated with antimicrobial thin films are proposed. Commercial polypropylene meshes were coated with metal-containing diamond-like carbon (Me-DLC) thin films by the magnetron sputtering technique. Several dissimilar metals (silver, cobalt, indium, tungsten, tin, aluminum, chromium, zinc, manganese, tantalum, and titanium) were tested and compositional analyses of each Me-DLC were performed by Rutherford backscattering spectrometry...
June 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28559743/adhesion-of-candida-spp-and-pichia-spp-to-wooden-surfaces
#19
Ružica Tomičić, Zorica Tomičić, Peter Raspor
Yeast adhesion to and biofilm formation on surfaces is present in many different environments. In food industry, biofilms may be a source of contaminations, causing food spoilage and reducing quality of products. Candida and Pichia are two common yeast genera involved in the spoilage of some food products. The aim of this study is to assess the potential of Candida and Pichia species to adhere to two types of wooden surfaces (smooth and rough), one of the materials typical for the food processing industry, and investigate the influence of surface roughness of wood on the degree of yeast adhesion...
March 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/28559266/an-immunomodulatory-peptide-that-confers-protection-in-an-experimental-candidemia-murine-model
#20
Camila G Freitas, Stella M F Lima, Mirna S Freire, Ana Paula C Cantuária, Nelson G O Júnior, Tatiane S Santos, Jéssica S Folha, Suzana M Ribeiro, Simoni C Dias, Taia M B Rezende, Patrícia Albuquerque, André M Nicola, César de la Fuente-Núñez, Robert E W Hancock, Octávio L Franco, Maria Sueli S Felipe
Fungal Candida species are commensals present in the mammalian skin and mucous membranes. Candida spp. are capable of breaching the epithelial barrier of immuno-compromised patients with neutrophil and cell-mediated immune dysfunctions, and it can also disseminate to multiple organs through the bloodstream. Here we examined the action of innate defence regulator (IDR)-1018, a 12-aminoacid residue peptide derived from bovine bactenecin (Bac2A), showed weak antifungal and anti-biofilm activity against C. albicans laboratory (ATCC 10231) and a clinical isolate (CI) strains (MICs of 32 μg...
May 30, 2017: Antimicrobial Agents and Chemotherapy
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