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https://www.readbyqxmd.com/read/29785832/lectin-microarray-combined-with-mass-spectrometry-identifies-haptoglobin-related-protein-hpr-as-a-potential-serologic-biomarker-for-separating-non-bacterial-pneumonia-from-bacterial-pneumonia-in-childhood
#1
Lin Yang, Zhou Yang, Lei Cheng, Juan Cheng, Li Cheng, Yangyang Sun, Wenting Li, Kai Song, Weichun Huang, Yong Yin, Shengce Tao, Qinghua Zhang
PURPOSE: Etiological diagnosis of pediatric patients with community-acquired pneumonia is difficult. For therapy, one of the major problems is the difficulty in separating bacterial pneumonia which would benefit from antibiotics from non-bacterial pneumonia. Therefore, we sought to identify potential biomarkers for distinguishing non-bacterial pneumonia from bacterial pneumonia. EXPERIMENTAL DESIGN: Lectin microarray containing 91 lectins was used to screen serums from pediatric patients with pneumonia...
May 22, 2018: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29784879/er-resident-protein-46-erp46-triggers-the-mannose-trimming-activity-of-er-degradation-enhancing-%C3%AE-mannosidase-like-protein-3-edem3
#2
Shangyu Yu, Shinji Ito, Ikuo Wada, Nobuko Hosokawa
Protein folding in the cell is regulated by several quality-control mechanisms. Correct folding of glycoproteins in the endoplasmic reticulum (ER) is tightly monitored by the recognition of glycan signals by lectins in the ER-associated degradation (ERAD) pathway. In mammals, mannose trimming from N-glycans is crucial for disposal of misfolded glycoproteins. The mannosidases responsible for this process are ER mannosidase I and ER degradation-enhancing α-mannosidase-like proteins (EDEMs). However, the molecular mechanism of mannose removal by EDEMs remains unclear, partly owing to the difficulty of reconstituting mannosidase activity in vitro Here, our analysis of EDEM3-mediated mannose-trimming activity on a misfolded glycoprotein revealed that ERp46, an ER-resident oxidoreductase, associates stably with EDEM3...
May 21, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29783620/binding-of-helicobacter-pylori-to-human-gastric-mucins-correlates-with-binding-of-tff1
#3
Ciara Dunne, Julie Naughton, Gina Duggan, Catherine Loughrey, Michelle Kilcoyne, Lokesh Joshi, Stephen Carrington, Helen Earley, Steffen Backert, Catherine Robbe Masselot, Felicity E B May, Marguerite Clyne
Helicobacter pylori binds to the gastric mucin, MUC5AC, and to trefoil factor, TFF1, which has been shown to interact with gastric mucin. We examined the interactions of TFF1 and H. pylori with purified gastrointestinal mucins from different animal species and from humans printed on a microarray platform to investigate whether TFF1 may play a role in locating H. pylori in gastric mucus. TFF1 bound almost exclusively to human gastric mucins and did not interact with human colonic mucins. There was a strong correlation between binding of TFF1 and H...
May 18, 2018: Microorganisms
https://www.readbyqxmd.com/read/29781989/bile-salt-induced-biofilm-formation-in-enteric-pathogens-techniques-for-identification-and-quantification
#4
Kourtney P Nickerson, Christina S Faherty
Biofilm formation is a dynamic, multistage process that occurs in bacteria under harsh environmental conditions or times of stress. For enteric pathogens, a significant stress response is induced during gastrointestinal transit and upon bile exposure, a normal component of human digestion. To overcome the bactericidal effects of bile, many enteric pathogens form a biofilm hypothesized to permit survival when transiting through the small intestine. Here we present methodologies to define biofilm formation through solid-phase adherence assays as well as extracellular polymeric substance (EPS) matrix detection and visualization...
May 6, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29781747/sialylation-pattern-in-lung-epithelial-cell-line-and-siglecs-expression-in-monocytic-thp-1-cells-as-cellular-indicators-of-cigarette-smoke-induced-pathology-in-vitro
#5
Przemyslaw Wielgat, Emil Trofimiuk, Robert Czarnomysy, Adam Holownia, Jan J Braszko
PURPOSE: Cellular response to cigarette smoke (CS) involves activation of recognition receptors resulting in changes in immune status, oxidative stress and cell turnover. We investigated the effects of CS on sialic acid-binding immunoglobulin type lectins (Siglecs) expression and their sialylated ligands in human immune and non-immune cells. METHODS: Human monocytes (THP-1) and epithelial cells (A549) were cultured in CS-conditioned medium (CSM). Expression of Siglec-8 and Siglec-5/Siglec-14 was analysed in THP-1 cells using flow cytometry...
May 21, 2018: Experimental Lung Research
https://www.readbyqxmd.com/read/29781274/the-synthesis-of-branched-trehalose-glycolipids-and-their-mincle-agonist-activity
#6
Jessie H Bird, Ashna A Khan, Naoya Nishimura, Sho Yamasaki, Mattie S M Timmer, Bridget L Stocker
The macrophage inducible C-type lectin (Mincle) is a pattern recognition receptor that recognises trehalose dimycolate (TDM), and trehalose dibehenate (TDB) and related trehalose diesters, and thus represents a promising target for the development of vaccine adjuvants based on the trehalose glycolipid scaffold. To this end, we report on the synthesis of a series of long-chain α-branched, β-modified trehalose monoesters and diesters to explore how glycolipid structure affects signalling through Mincle. Key steps in our synthetic strategy include a Fráter-Seebach α-alkylation to install the C20 aliphatic lipid on a malic acid derivative, and the formation of a β, γ-epoxide as an intermediate from which modifications to the β-position of the lipid can be made...
May 21, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29780375/effect-of-artinm-on-human-blood-cells-during-infection-with-paracoccidioides-brasiliensis
#7
Luciana P Ruas, Livia M Genaro, Amauri S Justo-Junior, Lilian O Coser, Lívia F de Castro, Plinio Trabasso, Ronei L Mamoni, Maria-Cristina Roque-Barreira, Maria-Heloisa S L Blotta
Infections caused by fungi are prominent in our environment and can be potentially fatal. paracoccidioidomycosis (PCM), caused by fungi of the Paracoccidioides genus, is the most frequent systemic mycosis in Brazil and the main cause of death among immunocompetent individuals. The antifungal therapy for PCM is usually effective but side effects and relapses are often reported. The latter could be avoided with alternative or complementary therapies aimed at boosting the immune response to combat this pathogen...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29778193/single-nucleotide-variants-in-innate-immune-genes-associated-with-salmonella-shedding-and-colonization-in-swine-on-commercial-farms
#8
Margaret H Ainslie-Garcia, Abdolvahab Farzan, Mohsen Jafarikia, Brandon N Lillie
Foodborne human salmonellosis is an important food safety concern worldwide. Food-producing animals are one of the major sources of human salmonellosis, and thus control of Salmonella at the farm level could reduce Salmonella spread in the food supply system. Genetic selection of pigs with resistance to Salmonella infection may be one way to control Salmonella on swine farms. The objective of this study was to investigate the association between genetic variants in the porcine innate immune system with on-farm Salmonella shedding and Salmonella colonization tested at slaughter...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778018/phytochemistry-and-pharmacology-of-anti-depressant-medicinal-plants-a-review
#9
REVIEW
Jeanette Martins, Brijesh S
Stress renders an individual to experience mental pressure and exhaustion which brings about feelings of anxiety, depression, anger and/or other negative emotions. Depression affects a person's state of mind, behaviour, health and is often associated with suicide. The use of anti-depressant drugs as therapeutic agents is associated with symptoms such as, delayed onset of action, side-effects, drug-drug and dietary interactions, sexual dysfunction, cardiac toxicity, etc. Thus, there is need to target these issues and improve current treatment options...
May 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29777873/cinnamaldehyde-protects-vsmcs-against-ox-ldl-induced-proliferation-and-migration-through-s-arrest-and-inhibition-of-p38-jnk-mapks-and-nf-%C3%AE%C2%BAb
#10
Weifeng Li, Wenbing Zhi, Jinmeng Zhao, Qing Yao, Fang Liu, Xiaofeng Niu
Cinnamaldehyde (Cin), as a traditional flavor constituent isolated from the bark of Cinnamonum cassia Presl, has been commonly used for - digestive, cardiovascular and immune system diseases. The pathology of vascular smooth muscle cells (VSMCs) accelerated the progression of atherosclerosis. In our study, we found that cinnamaldehyde significantly suppressed ox-LDL-induced VSMCs proliferation, migration and inflammatory cytokine overproduction, as well as foam cell formation in VSMCs and macrophages. Moreover, cinnamaldehyde inhibited the phosphorylation of p38, JNK and p65 NF-κB and increased heme oxygenase-1 (HO-1) activity...
May 16, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29777223/card9-s12n-facilitates-the-production-of-il-5-by-alveolar-macrophages-for-the-induction-of-type-2-immune-responses
#11
Xia Xu, Jin-Fu Xu, Guoxing Zheng, Hai-Wen Lu, Jie-Lin Duan, Wei Rui, Jian-Hong Guan, Li-Qing Cheng, Dan-Dan Yang, Ming-Chao Wang, Quan-Zhen Lv, Jian-Xiong Li, Xueqiang Zhao, Chun-Xia Chen, Peng Shi, Xin-Ming Jia, Xin Lin
The adaptor CARD9 functions downstream of C-type lectin receptors (CLRs) for the sensing of microbial infection, which leads to responses by the TH 1 and TH 17 subsets of helper T cells. The single-nucleotide polymorphism rs4077515 at CARD9 in the human genome, which results in the substitution S12N (CARD9S12N ), is associated with several autoimmune diseases. However, the function of CARD9S12N has remained unknown. Here we generated CARD9S12N knock-in mice and found that CARD9S12N facilitated the induction of type 2 immune responses after engagement of CLRs...
May 18, 2018: Nature Immunology
https://www.readbyqxmd.com/read/29775825/binding-capacity-of-mannose-binding-lectin-mbl-is-associated-with-the-severity-of-chronic-chagas-cardiomyopathy
#12
Elisa de A N Azevedo, Silvana Barreto, Raul Emídio de Lima, Romero Henrique Teixeira, George Diniz, Wilson Oliveira, Maria da Glória A M Cavalcanti, Yara M Gomes, Patrícia M M de F Moura, Clarice N L Morais
Chagas disease (CD) is a global problem. Currently, it affects approximately 15 million individuals in Latin America. It is well know that the human immune response is related to different clinical manifestations. Mannose binding lectin (MBL) plays an important role in innate immunity, and it mediates the phagocytosis and complement-mediated destruction of pathogens. The binding capacity is enhanced by the oligomerization of MBL. In this study, we evaluated the serum concentration and the binding capacity of MBL in patients with chronic chagasic cardiomyopathy...
May 15, 2018: Parasitology International
https://www.readbyqxmd.com/read/29775739/effects-of-environmental-factors-on-c-type-lectin-recognition-to-zooxanthellae-in-the-stony-coral-pocillopora-damicornis
#13
Zhi Zhou, Shuimiao Zhao, Junyi Ni, Yilu Su, Lingui Wang, Yanlai Xu
C-type lectin is a superfamily of Ca2+ -dependent carbohydrate-recognition proteins that play significant roles in nonself-recognition and pathogen clearance. In the present study, a C-type lectin (PdC-Lectin) was chosen from stony coral Pocillopora damicornis to understand its recognition characteristics to zooxanthellae. PdC-Lectin protein contained a signal peptide and a carbohydrate-recognition domain with EPN motif in Ca2+ -binding site 2. The PdC-Lectin recombinant protein was expressed and purified in vitro...
May 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29774302/ocular-histopathology-and-immunohistochemical-analysis-in-the-oldest-known-individual-with-autosomal-dominant-vitreoretinochoroidopathy
#14
Morton F Goldberg, Scott McLeod, Mark Tso, Kirk Packo, Malia Edwards, Imran A Bhutto, Rajkumar Baldeosingh, Charles Eberhart, Bernhard H F Weber, Gerard A Lutty
Purpose: To assess the immunohistochemical and histopathological changes in a subject with Autosomal Dominant Vitreoretinochoroidopathy (ADVIRC). Design: Case study. Participant: Ninety two year-old Caucasian male with ADVIRC. Methods: The subject was documented clinically for 54 Years. The retina/choroid complex of the right eye was evaluated with cryosections stained with hematoxylin and eosin or periodic acid schiff reagent...
April 2018: Ophthalmology Retina
https://www.readbyqxmd.com/read/29774107/clinical-outcome-of-hepatocellular-carcinoma-can-be-predicted-by-the-expression-of-hepatic-progenitor-cell-markers-and-serum-tumour-markers
#15
Satoshi Seino, Atsunori Tsuchiya, Yusuke Watanabe, Yuzo Kawata, Yuichi Kojima, Shunzo Ikarashi, Hiroyuki Yanai, Koji Nakamura, Daisuke Kumaki, Masaaki Hirano, Kazuhiro Funakoshi, Takashi Aono, Takeshi Sakai, Jun Sakata, Masaaki Takamura, Hirokazu Kawai, Satoshi Yamagiwa, Toshifumi Wakai, Shuji Terai
The high heterogeneity of hepatocellular carcinomas (HCCs) complicates stratification of HCC patients for treatment. Therefore, it is necessary to establish a comprehensive panel of HCC biomarkers related to tumour behaviour and cancer prognosis. Resected HCCs from 251 patients were stained for hepatic progenitor cell (HPC) markers epithelial cell adhesion molecule (EpCAM), neural cell adhesion molecule (NCAM), delta-like 1 homolog (DLK1), and cytokeratin 19 (CK19). Staining patterns were analysed for their prognostic association with relapse-free survival and overall survival...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29773815/the-trimeric-solution-structure-and-fucose-binding-mechanism-of-the-core-fucosylation-specific-lectin-phosl
#16
Kazuhiko Yamasaki, Tomoko Yamasaki, Hiroaki Tateno
The core α1-6 fucosylation-specific lectin from a mushroom Pholiota squarrosa (PhoSL) is a potential tool for precise diagnosis of cancers. This lectin consists of only 40 amino acids and can be chemically synthesized. We showed here that a synthesized PhoSL peptide formed a trimer by gel filtration and chemical cross-linking assays, and determined a structure of the PhoSL trimer by NMR. The structure possesses a β-prism motif with a three-fold rotational symmetry, where three antiparallel β-sheets are tightly connected by swapping of β-strands...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29772373/a-novel-c-type-lectin-from-the-sea-cucumber-apostichopus-japonicus-ajctl-2-with-preferential-binding-of-d-galactose
#17
Hui Wang, Zhuang Xue, Zhaoqun Liu, Weilin Wang, Feifei Wang, Ying Wang, Lingling Wang, Linsheng Song
C-type lectins (CTLs) are Ca2+ dependent carbohydrate-binding proteins that share structural homology in their carbohydrate-recognition domains (CRDs). In the present study, a novel CTL was identified from sea cucumber Apostichopus japonicus (named as AjCTL-2). The deduced amino acid sequence of AjCTL-2 was homologous to CTLs from other animals with the identities ranging from 33% to 40%. It contained a canonical signal peptide at the N-terminus, a low density lipoprotein receptor class A (LDLa), a C1r/C1s/Uegf/bone morphogenetic protein 1 (CUB), and a CRD with two motif Glu-Pro-Asn (EPN) and Trp-Asn-Asp (WND) in Ca2+ binding site 2...
May 14, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29771685/enterotoxigenic-escherichia-coli-blood-group-a-interactions-intensify-diarrheal-severity
#18
Pardeep Kumar, F Matthew Kuhlmann, Subhra Chakroborty, A Louis Bourgeois, Jennifer Foulke-Abel, Brunda Tumala, Tim J Vickers, David A Sack, Barbara DeNearing, Clayton D Harro, W Shea Wright, Jeffrey C Gildersleeve, Matthew A Ciorba, Srikanth Santhanam, Chad K Porter, Ramiro L Gutierrez, Michael G Prouty, Mark S Riddle, Alexander Polino, Alaullah Sheikh, Mark Donowitz, James M Fleckenstein
Enterotoxigenic Escherichia coli (ETEC) infections are highly prevalent in developing countries where clinical presentations range from asymptomatic colonization to severe cholera-like illness. The molecular basis for these varied presentations, that may involve strain-specific virulence features as well as host factors, have not been elucidated. We demonstrate that when challenged with ETEC strain H10407, originally isolated from a case of cholera-like illness, blood group A human volunteers developed severe diarrhea more frequently than individuals from other blood groups...
May 17, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29769410/human-anti-thrombospondin-type-1-domain-containing-7a-antibodies-induce-membranous-nephropathy-through-activation-of-lectin-complement-pathway
#19
Zheng Wang, Lu Wen, Yanna Dou, Zhanzheng Zhao
To investigate whether the human anti-thrombospondin type 1 domain-containing 7A (THSD7A) antibody-induced membranous nephropathy is mediated by activating lectin complement pathway. Automatic biochemical apparatus was used to assess renal function of mice. The serum levels of anti-THSD7A antibodies and complement were tested by using Enzyme-linked immunosorbent assay (ELISA). The expression level of THSD7A and Mannose-Binding Lectin (MBL) in clinical tissue, and the histological features of MN in mice were examined by immunochemical methods...
May 16, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29769321/lectin-mediated-binding-and-sialoglycans-of-porcine-surfactant-protein-d-synergistically-neutralize-influenza-a-virus
#20
Martin van Eijk, Michael J Rynkiewicz, Kshitij Khatri, Nancy Leymarie, Joseph Zaia, Mitchell R White, Kevan L Hartshorn, Tanya R Cafarella, Irma Van Die, Martin Hessing, Barbara A Seaton, Henk P Haagsman
Innate immunity is critical in the early containment of influenza A virus (IAV) infection, and surfactant protein D (SP-D) plays a crucial role in the pulmonary defense against IAV. In pigs, which are important intermediate hosts during the generation of pandemic IAVs, SP-D uses its unique carbohydrate recognition domain (CRD) to interact with IAV. An N-linked CRD-glycosylation provides interactions with the sialic acid binding site of IAV, and a tripeptide loop at the lectin binding site facilitates enhanced interactions with IAV glycans...
May 16, 2018: Journal of Biological Chemistry
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