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https://www.readbyqxmd.com/read/28223935/morus-alba-leaf-lectin-mll-sensitizes-mcf-7-cells-to-anoikis-by-inhibiting-fibronectin-mediated-integrin-fak-signaling-through-ras-and-activation-of-p-38-mapk
#1
Jayaram Saranya, Ganesan Shilpa, Kozhiparambil G Raghu, Sulochana Priya
Lectins are a unique class of carbohydrate binding proteins/glycoproteins, and many of them possess anticancer properties. They can induce cell cycle arrest and apoptosis, inhibit protein synthesis, telomerase activity and angiogenesis in cancer cells. In the present study, we have demonstrated the effect of Morus alba leaf lectin (MLL) on anoikis induction in MCF-7 cells. Anoikis induction in cancer cells has a significant role in preventing early stage metastasis. MLL treatment in monolayers of MCF-7 cells caused significant detachment of cells in a time and concentration dependent manner...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28223515/rational-design-of-adjuvants-targeting-the-c-type-lectin-mincle
#2
Alexiane Decout, Sandro Silva-Gomes, Daniel Drocourt, Sophie Barbe, Isabelle André, Francisco J Cueto, Thierry Lioux, David Sancho, Eric Pérouzel, Alain Vercellone, Jacques Prandi, Martine Gilleron, Gérard Tiraby, Jérôme Nigou
The advances in subunit vaccines development have intensified the search for potent adjuvants, particularly adjuvants inducing cell-mediated immune responses. Identification of the C-type lectin Mincle as one of the receptors underlying the remarkable immunogenicity of the mycobacterial cell wall, via recognition of trehalose-6,6'-dimycolate (TDM), has opened avenues for the rational design of such molecules. Using a combination of chemical synthesis, biological evaluation, molecular dynamics simulations, and protein mutagenesis, we gained insight into the molecular bases of glycolipid recognition by Mincle...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28221015/taking-orders-from-light-photo-switchable-working-inactive-smart-surfaces-for-protein-and-cell-adhesion
#3
Junji Zhang, Wenjing Ma, Xiao-Peng He, He Tian
Photo-responsive smart surfaces are promising candidates for a variety of applications in optoelectronics and sensing devices. The use of light as an order-signal provides advantages of remote and non-invasive control with high temporal and spatial resolutions. Modification of the photo-switches with target bio-macromolecules, such as peptides, DNA and small-molecules including folic acid derivatives and sugars, has recently become a popular strategy to empower the smart surfaces with an improved detection efficiency and specificity...
February 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28220817/a-simple-approach-for-preparation-of-affinity-matrices-simultaneous-purification-and-reversible-immobilization-of-a-streptavidin-mutein-to-agarose-matrix
#4
Sau-Ching Wu, Chris Wang, Dave Hansen, Sui-Lam Wong
SAVSBPM18 is an engineered streptavidin for affinity purification of both biotinylated biomolecules and recombinant proteins tagged with streptavidin binding peptide (SBP) tags. To develop a user-friendly approach for the preparation of the SAVSBPM18-based affinity matrices, a designer fusion protein containing SAVSBPM18 and a galactose binding domain was engineered. The galactose binding domain derived from the earthworm lectin EW29 was genetically modified to eliminate a proteolytic cleavage site located at the beginning of the domain...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28219890/local-tnfr1-signaling-licenses-murine-neutrophils-for-increased-tlr-dependent-cytokine-and-eicosanoid-production
#5
Jacques Deguine, Jessica Wei, Roman Barbalat, Karsten Gronert, Gregory M Barton
Neutrophils are generally the first immune cells recruited during the development of sterile or microbial inflammation. As these cells express many innate immune receptors with the potential to directly recognize microbial or endogenous signals, we set out to assess whether their functions are locally influenced by the signals present at the onset of inflammation. Using a mouse model of peritonitis, we demonstrate that neutrophils elicited in the presence of C-type lectin receptor ligands have an increased ability to produce cytokines, chemokines, and lipid mediators in response to subsequent TLR stimulation...
February 20, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28217370/tryptophan-a-gut-microbiota-derived-metabolites-regulating-inflammation
#6
REVIEW
Lucie Etienne-Mesmin, Benoit Chassaing, Andrew T Gewirtz
Inflammatory bowel diseases (IBD), which comprise Crohn's disease and ulcerative colitis, are chronic intestinal disorders with an increased prevalence and incidence over the last decade in many different regions over the world. The etiology of IBD is still not well defined, but evidence suggest that it results from perturbation of the homeostasis between the intestinal microbiota and the mucosal immune system, with the involvement of both genetic and environmental factors. Genome wide association studies, which involve large-scale genome-wide screening of potential polymorphism, have identified several mutations associated with IBD...
February 6, 2017: World Journal of Gastrointestinal Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28217098/quercetin-inhibits-lps-induced-inflammation-and-ox-ldl-induced-lipid-deposition
#7
Feng Xue, Xiaobo Nie, Jianping Shi, Qingxue Liu, Ziwei Wang, Xiting Li, Jinqiu Zhou, Jia Su, Mingming Xue, Wei-Dong Chen, Yan-Dong Wang
Aberrant activation of inflammation and excess accumulation of lipids play crucial role in the occurrence and progression of atherosclerosis (AS). Quercetin (QCT) has been tested effectively to cure AS. It is widely distributed in plant foods and has been proved to have potential antioxidative and anticancer activities. However, the underlying molecular mechanisms of OCT in AS are not completely understood. In the present study, we stimulated murine RAW264.7 cells with lipopolysaccharide (LPS) or oxidized low-density lipoproteins (ox-LDL) to mimic the development of AS...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28216633/pentraxin-3-ficolin-2-and-lectin-pathway-associated-serine-protease-masp-3-as-early-predictors-of-myocardial-infarction-the-hunt2-study
#8
Inga Thorsen Vengen, Tone Bull Enger, Vibeke Videm, Peter Garred
The lectin complement pathway is suggested to play a role in atherogenesis. Pentraxin-3 (PTX3), ficolin-1, ficolin-2, ficolin-3, MBL/ficolin/collectin-associated serine protease-3 (MASP-3) and MBL/ficolin/collectin-associated protein-1 (MAP-1) are molecules related to activation of the lectin complement pathway. We hypothesized that serum levels of these molecules may be associated with the incidence of myocardial infarction (MI). In a Norwegian population-based cohort (HUNT2) where young to middle-aged relatively healthy Caucasians were followed up for a first-time MI from 1995-1997 through 2008, the 370 youngest MI patients were matched by age (range 29-62 years) and gender to 370 controls...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28215876/in-vitro-lectin-binding-to-the-outer-surface-of-spirocerca-lupi-at-different-life-stages
#9
I Aroch, I Arogeti, A Marcovics, Y Spiegel, E Lavy
Spirocerca lupi is the esophageal nematode of dogs. Early, transient eosinophilia occurs in experimentally infected dogs, but is absent in advanced cases, suggesting that the nematode evades the dog's immune system. Lectins are proteins or glycoproteins of plant or animal origin, binding different saccharides, with varying specificities and avidities, used to characterize surface haptens in plant and animal parasitic helminths. This study investigated the in vitro binding of six lectins (Concanavalin A [ConA], wheat germ agglutinin [WGA], peanut agglutinin [PNA], soybean agglutinin [SBA], Dolichus biflorus agglutinin [DBA] and Ulex earopaeus agglutinin I [UEA]) to the surface of S...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28215157/glycans-in-infectious-diseases-a-molecular-recognition-perspective
#10
Luca Unione, Ana Gimeno, Pablo Valverde, Ilaria Calloni, Helena Coelho, Stefania Mirabella, Ana Poveda, Ana Arda, Jesús Jiménez-Barbero
The importance of glycans in infection processes has been demonstrated during the past few years. Indeed, it is today a hot topic with diverse branches and perspectives. The field is therefore attracting increased attention and it is expected that will provide spectacular developments. In this non-exhaustive review, we have focused on the molecular recognition aspects that involve the participation of glycans in different infection processes of paramount importance, with special attention to the applications of NMR spectroscopy and other biophysical techniques...
February 16, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28213038/hydrolysate-from-a-mixture-of-legume-flours-with-antifungal-activity-as-an-ingredient-for-prolonging-the-shelf-life-of-wheat-bread
#11
Carlo Giuseppe Rizzello, Michela Verni, Stefano Bordignon, Valerio Gramaglia, Marco Gobbetti
Aiming at identifying antifungal compounds from plant matrices to be used as ingredients in the bakery industry, a water/salt-soluble extract (WSE) was produced from a legume enzyme hydrolysate, consisting of a mixture of pea, lentil, and faba bean flours, and assayed towards Penicillium roqueforti DPPMAF1. Agar diffusion assays allowed the selection of the optimal processing conditions for hydrolysis. As shown by hyphal radial growth rate, the inhibition was observed towards several fungi, including Aspergillus parasiticus CBS971...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28212932/purification-and-characterisation-of-a-pronase-inducible-lectin-isolated-from-human-serum
#12
Beulaja Manikandan, Manikandan Ramar, Thiagarajan Raman, Mullainadhan Periasamy, Arumugam Munusamy
A new lectin was purified to electrophoretic homogeneity from pronase treated human serum by a single-step of affinity chromatography on concanavalin A-Sepharose 4B. The isolated lectin agglutinated five types of vertebrate RBC, with highest titer against hen RBC. This activity was independent of divalent cations, insensitive to EDTA and specific to mannosamine, glucosamine as well as galactosamine. This lectin gave a single symmetrical peak in its native form with a molecular mass estimate of 6kDa in FPLC analysis and 6...
February 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28210565/label-free-proteome-analysis-of-plasma-from-patients-with-breast-cancer-stage-specific-protein-expression
#13
Marina Duarte Pinto Lobo, Frederico Bruno Mendes Batista Moreno, Gustavo Henrique Martins Ferreira Souza, Sara Maria Moreira Lima Verde, Renato de Azevedo Moreira, Ana Cristina de Oliveira Monteiro-Moreira
Breast cancer is one of the most commonly diagnosed types of cancer among women. Breast cancer mortality rates remain high probably because its diagnosis is hampered by inaccurate detection methods. Since changes in protein expression as well as modifications in protein glycosylation have been frequently reported in cancer development, the aim of this work was to study the differential expression as well as modifications of glycosylation of proteins from plasma of women with breast cancer at different stages of disease (n = 30) compared to healthy women (n = 10)...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28209773/mannan-binding-lectin-a-serum-collectin-suppresses-t-cell-proliferation-via-direct-interaction-with-cell-surface-calreticulin-and-inhibition-of-proximal-t-cell-receptor-signaling
#14
Na Zhao, Jie Wu, Simin Xiong, Liyun Zhang, Xiao Lu, Shangliang Chen, Qifeng Wu, Hailan Wang, Ying Liu, Zhengliang Chen, Daming Zuo
Mannan binding lectin (MBL), initially described to activate the complement pathway, is also known to be involved in the pathogenesis of autoimmune diseases. We report a thus far unknown function of MBL as a suppressor of T-cell activation. MBL markedly inhibited T-cell proliferation induced by anti-CD3 and anti-CD28 antibodies. Moreover, the presence of MBL during T-cell priming interfered with proximal T-cell receptor signaling by decreasing phosphorylation of Lck, ZAP-70, and LAT. MBL bound to T cells through interaction between the collagen-like region of MBL and calreticulin (CRT) expressed on the T-cell surface...
February 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28208623/fluorescence-lectin-bar-coding-of-glycoconjugates-in-the-extracellular-matrix-of-biofilm-and-bioaggregate-forming-microorganisms
#15
Thomas R Neu, Ute Kuhlicke
Microbial biofilm systems are defined as interface-associated microorganisms embedded into a self-produced matrix. The extracellular matrix represents a continuous challenge in terms of characterization and analysis. The tools applied in more detailed studies comprise extraction/chemical analysis, molecular characterization, and visualisation using various techniques. Imaging by laser microscopy became a standard tool for biofilm analysis, and, in combination with fluorescently labelled lectins, the glycoconjugates of the matrix can be assessed...
February 10, 2017: Microorganisms
https://www.readbyqxmd.com/read/28207759/investigations-on-therapeutic-glucocerebrosidases-through-paired-detection-with-fluorescent-activity-based-probes
#16
Wouter W Kallemeijn, Saskia Scheij, Sascha Hoogendoorn, Martin D Witte, Daniela Herrera Moro Chao, Cindy P A A van Roomen, Roelof Ottenhoff, Herman S Overkleeft, Rolf G Boot, Johannes M F G Aerts
Deficiency of glucocerebrosidase (GBA) causes Gaucher disease (GD). In the common non-neuronopathic GD type I variant, glucosylceramide accumulates primarily in the lysosomes of visceral macrophages. Supplementing storage cells with lacking enzyme is accomplished via chronic intravenous administration of recombinant GBA containing mannose-terminated N-linked glycans, mediating the selective uptake by macrophages expressing mannose-binding lectin(s). Two recombinant GBA preparations with distinct N-linked glycans are registered in Europe for treatment of type I GD: imiglucerase (Genzyme), contains predominantly Man(3) glycans, and velaglucerase (Shire PLC) Man(9) glycans...
2017: PloS One
https://www.readbyqxmd.com/read/28206701/differential-responses-of-helicoverpa-armigera-c-type-immunlectin-genes-to-the-endoparasitoid-campoletis-chlorideae
#17
Xiong-Ya Wang, Su-Fen Bai, Xin Li, Shi-Heng An, Xin-Ming Yin, Xian-Chun Li
The C-type lectins mediate nonself recognition in insects. The previous studies focused on host immunlectin response to bacterial infection; however, the molecular basis of immunlectin reactions to endoparasitoids has not been elucidated. The present study investigated the effect of parasitization by Campoletis chlorideae on hemagglutination activity (HA; defined as the ability of lectin to agglutinate erythrocytes or other cells), and transcriptional expression of C-type immunlectin genes in the larval host, Helicoverpa armigera...
February 16, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28205391/influence-of-iodine-in-excess-on-seminiferous-tubular-structure-and-epididymal-sperm-character-in-male-rats
#18
Amar K Chandra, Arijit Chakraborty
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress in the testis. However, information on the possible effect of iodine in excess on spermatozoa found less. In the present investigation flow cytometric techniques and scanning electron microscopy (SEM) have been used to study the spermatozoal functional as well as structural status under the influence of excess iodine; generation of ROS in the spermatozoa as evident by DCFDA, altered acrosomal integrity as observed by fluorescence lectin staining method and depolarized mitochondrial membrane potential (ΔΨm ) noticed by JC-1 staining...
February 16, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28205244/spatial-organization-of-the-three-component-lichen-peltigera-aphthosa-in-functional-terms
#19
Konstantin Chekanov, Alexandr Feoktistov, Elena Lobakova
The cephalolichen Peltigera aphthosa (L.) Willd. is characterized by lateral heterogeneity, which manifests itself in the presence of three thallus zones, referred to as the apical, basal and medial zone. These zones differ in terms of interaction between lichen bionts and their physiological activity. The apical thallus zone is more efficient in establishing a contact with cyanobacteria, due to a higher lectin content and a larger overall thallus surface area due to the presence of numerous mycobiont hyphae...
February 16, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28203237/activation-of-the-wnt-pathway-by-mycobacterium-tuberculosis-a-wnt-wnt-situation
#20
REVIEW
Tomás Villaseñor, Edgardo Madrid-Paulino, Rafael Maldonado-Bravo, Antonio Urbán-Aragón, Leonor Pérez-Martínez, Gustavo Pedraza-Alva
Mycobacterium tuberculosis (M. tuberculosis), an intracellular pathogenic Gram-positive bacterium, is the cause of tuberculosis (TB), a major worldwide human infectious disease. The innate immune system is the first host defense against M. tuberculosis. The recognition of this pathogen is mediated by several classes of pattern recognition receptors expressed on the host innate immune cells, including Toll-like receptors, Nod-like receptors, and C-type lectin receptors like Dectin-1, the Mannose receptor, and DC-SIGN...
2017: Frontiers in Immunology
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