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https://www.readbyqxmd.com/read/29145436/scrambled-eggs-proteomic-portraits-and-novel-biomarkers-of-egg-quality-in-zebrafish-danio-rerio
#1
Ozlem Yilmaz, Amélie Patinote, Thao Vi Nguyen, Emmanuelle Com, Regis Lavigne, Charles Pineau, Craig V Sullivan, Julien Bobe
Egg quality is a complex biological trait and a major determinant of reproductive fitness in all animals. This study delivered the first proteomic portraits of egg quality in zebrafish, a leading biomedical model for early development. Egg batches of good and poor quality, evidenced by embryo survival for 24 h, were sampled immediately after spawning and used to create pooled or replicated sample sets whose protein extracts were subjected to different levels of fractionation before liquid chromatography and tandem mass spectrometry...
2017: PloS One
https://www.readbyqxmd.com/read/29143694/cryo-scanning-electron-microscopy-sem-and-scanning-transmission-electron-microscopy-stem-in-sem-for-bio-and-organo-mineral-interface-characterization-in-the-environment
#2
Guillaume Wille, Jennifer Hellal, Patrick Ollivier, Annie Richard, Agnes Burel, Louis Jolly, Marc Crampon, Caroline Michel
Understanding biofilm interactions with surrounding substratum and pollutants/particles can benefit from the application of existing microscopy tools. Using the example of biofilm interactions with zero-valent iron nanoparticles (nZVI), this study aims to apply various approaches in biofilm preparation and labeling for fluorescent or electron microscopy and energy dispersive X-ray spectrometry (EDS) microanalysis for accurate observations. According to the targeted microscopy method, biofilms were sampled as flocs or attached biofilm, submitted to labeling using 4',6-diamidino-2-phenylindol, lectins PNA and ConA coupled to fluorescent dye or gold nanoparticles, and prepared for observation (fixation, cross-section, freezing, ultramicrotomy)...
November 16, 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/29142605/aviscumine-a-recombinant-ribosomal-inhibitor-increases-the-antitumor-activity-of-natural-killer-cells
#3
Gabriele Gamerith, Arno Amann, Bettina Schenk, Thomas Auer, Hans Lentzen, Dirk O Mügge, Katharina M Cima, Judith Löffler-Ragg, Wolfgang Hilbe, Heinz Zwierzina
Aviscumine, a recombinant lectin I, has been identified as an immunomodulatory agent within a new class of ribotoxic stress-inducing anticancer substances that have demonstrated efficacy in phase I/II trials. The aim of the present study was to elucidate the presumed effect of aviscumine on enhancing human natural killer (NK) cell antitumor cytotoxicity. To measure the effect of aviscumine on human NK cell cytotoxicity, chromium-51-release assays against K-562 cells were performed with isolated NK cells from the whole blood of 34 healthy volunteers...
November 2017: Oncology Letters
https://www.readbyqxmd.com/read/29142050/collectin-11-promotes-the-development-of-renal-tubulointerstitial-fibrosis
#4
Weiju Wu, Chengfei Liu, Conrad A Farrar, Liang Ma, Xia Dong, Steven H Sacks, Ke Li, Wuding Zhou
Collectin-11 is a recently described soluble C-type lectin, a pattern recognition molecule of the innate immune system that has distinct roles in host defense, embryonic development, and acute inflammation. However, little is known regarding the role of collectin-11 in tissue fibrosis. Here, we investigated collectin-11 in the context of renal ischemia-reperfusion injury. Compared with wild-type littermate controls, Collec11 deficient (CL-11(-/-) ) mice had significantly reduced renal functional impairment, tubular injury, renal leukocyte infiltration, renal tissue inflammation/fibrogenesis, and collagen deposition in the kidneys after renal ischemia-reperfusion injury...
November 15, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29141557/halilectin-3-a-lectin-from-the-marine-sponge-haliclona-caerulea-induces-apoptosis-and-autophagy-in-human-breast-cancer-mcf7-cells-through-caspase-9-pathway-and-lc3-ii-protein-expression-haliclona-caerulea-induces-apoptosis-and-autophagy-in-human-breast-cancer
#5
Paula A Videira, Luiz Gonzaga do Nascimento-Neto, Maria Guadalupe Cabral, Romulo Farias Carneiro, Francisco Vassiliepe Sousa Arruda, Zelia Silva, Celso Shiniti Nagano, Alexandre Holanda Sampaio, Edson Holanda Teixeira
BACKGROUND: An ideal strategy for cancer treatment is the specific induction of tumor cell death, sparing normal cells. Marine sponges are rich biological reservoirs of biomolecules, especially lectins, which have attracted considerable attention due to potential biological effect on human cells. Lectins are proteins that bind specific carbohydrate signatures and some gained further interest for their capacity to bind tumor associated carbohydrates antigens and induce tumor cell apoptosis...
November 13, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29137999/structural-and-functional-changes-of-fibrinogen-due-to-aging
#6
Nikola Gligorijević, Martina Zámorová Križáková, Ana Penezić, Jaroslav Katrlík, Olgica Nedić
Different factors affect coagulation process. Since fibrinogen is the main coagulation factor, the influence of aging on fibrinogen structure and function was investigated in this study. Fibrinogen was isolated from plasma obtained from healthy persons in the age range 21-83 and examined. Lectin microarray analysis demonstrated increased glycosylation of fibrinogen due to aging, with predominant increase in high-mannose or hybrid type N-glycans, as well as tri-/tetraantennary complex N-glycans with greater content of galactose and N-acetylglucosamine residues...
November 11, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29136334/identification-of-plasma-membrane-glycoproteins-specific-to-human-glioblastoma-multiforme-cells-using-lectin-arrays-and-lc-ms-ms
#7
YaeEun Park, Jeonghun Yeom, YoungSoo Kim, Hye Jin Lee, Ki-Cheol Han, SeungTaek Lee, Cheolju Lee, Ji Eun Lee
Glioblastoma, also known as glioblastoma multiforme (GBM), is the most malignant type of brain cancer and has poor prognosis with a median survival of less than one year. While the structural changes of tumor cell surface carbohydrates are known to be associated with invasive behavior of tumor cells, the cell surface glycoproteins to differentiate the low- and high-grade glioma cells can be potential diagnostic markers and therapeutic targets for GBMs. In the present study, lectin arrays consisting of eight lectins were employed to explore cell surface carbohydrate expression patterns on low-grade oligodendroglioma cells (Hs683) and GBM cells (T98G)...
November 14, 2017: Proteomics
https://www.readbyqxmd.com/read/29134205/towards-the-electrochemical-diagnostic-of-influenza-virus-development-of-a-graphene-au-hybrid-nanocomposite-modified-influenza-virus-biosensor-based-on-neuraminidase-activity
#8
Ülkü Anik, Yudum Tepeli, Maher Sayhi, Jihene Nsiri, Mohamed Fethi Diouani
An effective electrochemical influenza A biosensor based on a graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10(-8) U mL(-1) and 10(-1) U mL(-1) was found with a relative standard deviation value of 3...
November 14, 2017: Analyst
https://www.readbyqxmd.com/read/29134199/rapid-directed-transport-of-dc-sign-clusters-in-the-plasma-membrane
#9
Ping Liu, Violetta Weinreb, Marc Ridilla, Laurie Betts, Pratik Patel, Aravinda M de Silva, Nancy L Thompson, Ken Jacobson
C-type lectins, including dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), are all-purpose pathogen receptors that exist in nanoclusters in plasma membranes of dendritic cells. A small fraction of these clusters, obvious from the videos, can undergo rapid, directed transport in the plane of the plasma membrane at average speeds of more than 1 μm/s in both dendritic cells and MX DC-SIGN murine fibroblasts ectopically expressing DC-SIGN. Surprisingly, instantaneous speeds can be considerably greater...
November 2017: Science Advances
https://www.readbyqxmd.com/read/29133924/bio-detoxification-of-ricin-in-castor-bean-ricinus-communis-l-seeds
#10
Natália L Sousa, Glaucia B Cabral, Pabline M Vieira, Aisy B Baldoni, Francisco J L Aragão
Ricin is a highly toxic ribosome-inactivating lectin occurring in the seeds of castor bean (Ricinus communis L.). Castor bean grows throughout tropical and sub-tropical regions and is a very important crop due to its high seed content of ricinoleic acid, an unusual fatty acid, which has several industrial applications. However, due to the presence of the toxin, castor bean can cause death after the exposure of animals to low doses of ricin through skin contact, injection, inhalation or oral routes. Aiming to generate a detoxified genotype, we explored the RNAi concept in order to silence the ricin coding genes in the endosperm of castor bean seeds...
November 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29133252/l-rhamnose-binding-lectins-rbls-in-nile-tilapia-oreochromis-niloticus-characterization-and-expression-profiling-in-mucosal-tissues
#11
Dongdong Zhang, Benjamin H Beck, Haitham Mohammed, Honggang Zhao, Wilawan Thongda, Zhi Ye, Qifan Zeng, Craig Shoemaker, S Adam Fuller, Eric Peatman
Rhamnose-binding lectins (RBLs) are crucial elements associated with innate immune responses to infections and have been characterized from a variety of teleost fishes. Given the importance of RBL in teleost fishes, we sought to study the diversity and expression profiles of RBLs in an important cultured fish, Nile tilapia (Oreochromis niloticus) following experimental infection with Streptococcus agalactiae, a major cause of streptococcosis in farmed tilapia. In this study, four predicted RBL genes were identified from Nile tilapia and were designated as OnRBL3a, OnRBL3b, OnRBL3c, and OnRBL3d...
November 10, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29133078/enteric-delivery-of-regenerating-family-member-3-alpha-alters-the-intestinal-microbiota-and-controls-inflammation-in-mice-with-colitis
#12
Marion Darnaud, Alexandre Dos Santos, Patrick Gonzalez, Sandrine Augui, Claire Lacoste, Christophe Desterke, Gert De Hertogh, Emma Valentino, Emilie Braun, Jinzi Zheng, Raphael Boisgard, Christel Neut, Laurent Dubuquoy, Franck Chiappini, Didier Samuel, Patricia Lepage, Francesca Guerrieri, Joel Doré, Christian Bréchot, Nicolas Moniaux, Jamila Faivre
BACKGROUND & AIMS: Paneth cell dysfunction causes deficiencies in intestinal C-type lectins and antimicrobial peptides, which leads to dysbiosis of the intestinal microbiota, alters the mucosal barrier, and promotes development of inflammatory bowel diseases. We investigated whether transgenic expression of the human regenerating family member 3 alpha gene (REG3A) alters the fecal microbiota and affects development of colitis in mice. METHODS: We performed studies with C57BL/6 mice that express human regenerating family member 3 alpha (hREG3A) in hepatocytes, via the albumin gene promoter...
November 10, 2017: Gastroenterology
https://www.readbyqxmd.com/read/29131842/positive-selection-and-comparative-molecular-evolution-of-reproductive-proteins-from-new-zealand-tree-weta-orthoptera-hemideina
#13
Victoria G Twort, Alice B Dennis, Duckchul Park, Kathryn F Lomas, Richard D Newcomb, Thomas R Buckley
Animal reproductive proteins, especially those in the seminal fluid, have been shown to have higher levels of divergence than non-reproductive proteins and are often evolving adaptively. Seminal fluid proteins have been implicated in the formation of reproductive barriers between diverging lineages, and hence represent interesting candidates underlying speciation. RNA-seq was used to generate the first male reproductive transcriptome for the New Zealand tree weta species Hemideina thoracica and H. crassidens...
2017: PloS One
https://www.readbyqxmd.com/read/29131134/mannose-binding-lectin-is-not-acting-as-a-biomarker-for-the-progression-of-pre-invasive-lesions-of-invasive-cervical-cancer
#14
Carlos Afonso Maestri, Renato Nisihara, Guilherme P Ramos, Hellen Weinschutz Mendes, Iara Messias-Reason, Newton Sérgio de Carvalho
OBJECTIVE: To evaluate serum concentrations of mannose binding lectin (MBL) in women presenting with different human papilloma virus (HPV)-associated cervical lesions. SUBJECTS AND METHODS: A total of 364 women, who underwent screening for cervical cancer or treatment at Erasto Gaertner Cancer Hospital (HEG), Curitiba, Southern region, Brazil, were enrolled in the study. Based on latest cervical colposcopy-guided biopsy results, the women were divided into four groups: cervical intraepithelial neoplasia (CIN) CIN-I (n=54); CIN-II (n=72); CIN-III (n=145) and invasive cancer (n=93)...
November 7, 2017: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/29130773/alteration-of-salivary-glycopatterns-in-oral-lichen-planus
#15
Liang Fang, Qing Liu, Pengfei He, Xinwen Wang, Ying Wang, Minghui Wei, Lihua Chen
BACKGROUND: Oral lichen planus (OLP) is one of the most common oral mucosal lesions affecting 0.5% to 2% of the adult population. It is difficult to distinguish between OLP and other oral mucosal diseases. Structural changes in the glycans of saliva proteins might be reliable indicators of OLP. However, little is known about the alteration of salivary glycopatterns during OLP. OBJECTIVE: We aimed to investigate the alterations of salivary protein glycosylation related to OLP...
November 13, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/29129804/recent-advances-in-galactose-engineered-nanocarriers-for-the-site-specific-delivery-of-sirna-and-anticancer-drugs
#16
REVIEW
Ashay Jain, Atul Jain, Prahlad Parajuli, Vijay Mishra, Gargi Ghoshal, Bhupinder Singh, Uma Shankar Shivhare, Om Prakash Katare, Prashant Kesharwani
Galactosylated nanocarriers have recently emerged as viable and versatile tools to deliver drugs at an optimal rate specifically to their target tissues or cells, thus maximizing their therapeutic benefits while circumventing off-target effects. The abundance of lectin receptors on cell surfaces makes the galactosylated carriers suitable for the targeted delivery of bioactives. Additionally, tethering of galactose (GAL) to various carriers, including micelles, liposomes, and nanoparticles (NPs), might also be appropriate for drug delivery...
November 10, 2017: Drug Discovery Today
https://www.readbyqxmd.com/read/29129586/mannose-binding-lectin-and-its-roles-in-immune-responses-in-grass-carp-ctenopharyngodon-idella-against-aeromonas-hydrophila
#17
Yunfei Dang, Xinzhan Meng, Shentong Wang, Lisen Li, Meng Zhang, Moyan Hu, Xiaoyan Xu, Yubang Shen, Liqun Lv, Rongquan Wang, Jiale Li
The complement system is a crucial component of the innate immune system that links innate and adaptive immunity via four pathways. Mannose-binding lectin (MBL), the initiating molecule of the lectin pathway, plays a significant role in the innate immune system in mammals and fish. Herein, we identified an MBL homolog (gcMBL) in grass carp (Ctenopharyngodon idella). The full-length 948 bp gcMBL cDNA includes a 741 bp open reading frame encoding a 246 amino acid protein with a signal peptide, collagen triple helix repeat domain, and a C-type lectin-like/link domain...
November 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29127943/quantum-dot-cramoll-lectin-as-novel-conjugates-to-glycobiology
#18
Cássia R A Cunha, Camila G Andrade, Maria I A Pereira, Paulo E Cabral Filho, Luiz B Carvalho, Luana C B B Coelho, Beate S Santos, Adriana Fontes, Maria T S Correia
The optical properties of quantum dots (QDs) make them useful tools for biology, especially when combined with biomolecules such as lectins. QDs conjugated to lectins can be used as nanoprobes for carbohydrate expression analysis, which can provide valuable information about glycosylation changes related to cancer and pathogenicity of microorganisms, for example. In this study, we evaluated the best strategy to conjugate Cramoll lectin to QDs and used the fluorescent labeling of Candida albicans cells as a proof-of-concept...
October 25, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29127846/supramolecular-glycorhodamine-polymer-dot-ensembles-for-the-homogeneous-fluorogenic-analysis-of-lectins
#19
Chang-Zheng Wang, Xiao-Peng He
We have developed a new series of glycoprobe-polymer dot ensembles for the fluorogenic, homogeneous detection of lectins. Electrostatic self-assembly between positively charged rhodamine-based glycosides and negatively charged poly(3-hexylthiophene-2,5-diyl)/poly(styrene-co-maleic anhydride) polymer dots produces the ensembles with a quenched fluorescence. Fluorescence spectroscopy showed that the ensembles exhibited a concentration-dependent fluorescence enhancement with selective lectins over a range of unselective lectins and proteins...
November 1, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29127069/lectin-conjugated-ph-responsive-mesoporous-silica-nanoparticles-for-targeted-bone-cancer-treatment
#20
Marina Martínez-Carmona, Daniel Lozano, Montserrat Colilla, María Vallet-Regí
A novel multifunctional nanodevice based in doxorubicin (DOX)-loaded mesoporous silica nanoparticles (MSNs) as nanoplatforms for the assembly of different building blocks has been developed for bone cancer treatment. These building blocks consists of: i) a polyacrylic acid (PAA) capping layer grafted to MSNs via an acid-cleavable acetal linker, to minimize premature cargo release and provide the nanosystem of pH-responsive drug delivery ability; and ii) a targeting ligand, the plant lectin concanavalin A (ConA), able to selectively recognize, bind and internalize owing to certain cell-surface glycans, such as sialic acids (SA), overexpressed in given tumor cells...
November 7, 2017: Acta Biomaterialia
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