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https://www.readbyqxmd.com/read/28636161/infinity-sperm-storage-the-gift-that-keeps-on-giving
#1
Gauthier Schang, Beatriz Fernandez-Fuertes, Samantha C Lean, Alexandra L Nguyen, Osvaldo Bogado Pascottini
Female Drosophila retain sperm for up to two weeks post-copulation in a network of storage organs includng the bursa, the seminal receptacle, and the spermathecae. This image shows the seminal receptacle of a Drosophila melanogaster female, 2-hrs post-copulation between a LHM (wild-type) female with a Protamine B (green), β-Tubulin (blue) fluorescently tagged male, whose sperm can be visualized within the female. This 'infinity' structure of the seminal receptacle resembles the tubular coil that allows for the storage of very long sperm relative to female body size -up to 20 times the length of the female in some Drosophila species...
June 21, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28636039/a-convenient-method-for-multicolour-labelling-of-proteins-with-bodipy-fluorophores-via-tyrosine-residues
#2
Miffy H Y Cheng, Huguette Savoie, Francesca Bryden, Ross W Boyle
Fluorescence is an essential imaging modality for labelling and visualising cells and sub-cellular structures. Multicolour labelling is especially challenging due to differences in physicochemical and photophysical behaviour of structurally unrelated fluorophores in the heterogeneous environments found in sub-cellular compartments. Herein, we report the conjugation of three azide-bearing BODIPYs with similar core structures but widely different emission wavelengths (green, red and NIR) to tyrosine residues of a model globular protein (BSA) via a common linking methodology...
June 21, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28635963/single-molecule-analysis-of-steroid-receptor-and-cofactor-action-in-living-cells
#3
Ville Paakinaho, Diego M Presman, David A Ball, Thomas A Johnson, R Louis Schiltz, Peter Levitt, Davide Mazza, Tatsuya Morisaki, Tatiana S Karpova, Gordon L Hager
Population-based assays have been employed extensively to investigate the interactions of transcription factors (TFs) with chromatin and are often interpreted in terms of static and sequential binding. However, fluorescence microscopy techniques reveal a more dynamic binding behaviour of TFs in live cells. Here we analyse the strengths and limitations of in vivo single-molecule tracking and performed a comprehensive analysis on the intranuclear dwell times of four steroid receptors and a number of known cofactors...
June 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28635650/the-application-of-heptamethine-cyanine-dye-dz-1-and-indocyanine-green-for-imaging-and-targeting-in-xenograft-models-of-hepatocellular-carcinoma
#4
Caiqin Zhang, Yong Zhao, He Zhang, Xue Chen, Ningning Zhao, Dengxu Tan, Hai Zhang, Changhong Shi
Near infrared fluorescence (NIRF) imaging has strong potential for widespread use in noninvasive tumor imaging. Indocyanine green (ICG) is the only Food and Drug Administration (FDA) -approved NIRF dye for clinical diagnosis; however, it is unstable and poorly targets tumors. DZ-1 is a novel heptamethine cyanine NIRF dye, suitable for imaging and tumor targeting. Here, we compared the fluorescence intensity and metabolism of DZ-1 and ICG. Additionally, we assayed their specificities and abilities to target tumor cells, using cultured hepatocellular carcinoma (HCC) cell lines, a nude mouse subcutaneous xenograft model of liver cancer, and a rabbit orthotopic transplantation model...
June 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28635419/multimodal-imaging-gd-nanoparticles-functionalized-with-pittsburgh-compound-b-or-a-nanobody-for-amyloid-plaques-targeting
#5
Jonathan Pansieri, Marie Plissonneau, Nathalie Stransky-Heilkron, Mireille Dumoulin, Laurence Heinrich-Balard, Pascaline Rivory, Jean-François Morfin, Eva Toth, Maria Joao Saraiva, Eric Allémann, Olivier Tillement, Vincent Forge, François Lux, Christel Marquette
AIM: Gadolinium-based nanoparticles were functionalized with either the Pittsburgh compound B or a nanobody (B10AP) in order to create multimodal tools for an early diagnosis of amyloidoses. MATERIALS & METHODS: The ability of the functionalized nanoparticles to target amyloid fibrils made of β-amyloid peptide, amylin or Val30Met-mutated transthyretin formed in vitro or from pathological tissues was investigated by a range of spectroscopic and biophysics techniques including fluorescence microscopy...
June 21, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28634606/a-new-aie-multi-block-polyurethane-copolymer-material-for-subcellular-microfilament-imaging-in-living-cells
#6
Yu-Qing Niu, Tao He, Jun Song, Si-Ping Chen, Xiang-Yu Liu, Zhi-Gang Chen, Ying-Jie Yu, Shi-Guo Chen
A multi-block fluorescent amphiphilic polyurethane copolymer (TPE-PU), self-assembling into hairy, water-soluble micelles, is used as a subcellular microfilament probe in living cells.
June 21, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28634228/spatial-mapping-and-profiling-of-metabolite-distributions-during-germination
#7
Adam D Feenstra, Liza E Alexander, Zhihong Song, Andrew R Korte, Marna Yandeau-Nelson, Basil J Nikolau, Young-Jin Lee
Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids...
June 20, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28633520/ion-selective-nanosensor-for-photoacoustic-and-fluorescence-imaging-of-potassium
#8
Chang Heon Lee, Jeff Folz, Wuliang Zhang, Janggun Jo, Joel W Y Tan, Xueding Wang, Raoul Kopelman
Ion-selective optodes (ISOs), the optical analog of ion-selective electrodes, have played an increasingly important role in chemical and biochemical analysis. Here we extend this technique to ion-selective photo-acoustic optodes (ISPAOs) that serve at the same time as fluorescence-based ISOs, and apply it specifically to potassium (K+). Notably, the potassium ion is one of the most abundant cations in biological systems, involved in numerous physiological and pathological processes. Furthermore, it has been recently reported that the presence of abnormal extracellular potassium concentrations in tumors suppresses the immune responses and thus suppresses immunotherapy...
June 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28632960/snap-tag-based-subcellular-proteins-labeling-and-fluorescent-imaging-with-naphthalimides
#9
Chao Wang, Xinbo Song, Yi Xiao
Genetically encoded technologies provide methods for specific labeling and imaging of proteins, which is essential for the understanding of their subcellular localization and function. Herein, we employ naphthalimide, an efficient two-photon fluorophore, to develop O6-benzylguanine (BG) derivatives for specific labeling of subcellular proteins and fluorescent imaging through the SNAP-tag. The three naphthalimide-BG derivatives, TNI-BG, QNI-BG and ONI-BG are conveniently synthesized through modular "click chemistry" with high yields...
June 20, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28631299/carbazole-azine-based-fluorescence-off-on-sensor-for-selective-detection-of-cu-2-and-its-live-cell-imaging
#10
Denzil Britto Christopher Leslee, Sekar Karuppannan, Karmegam Muthu Vengaian, Sivaraman Gandhi, Singaravadivel Subramanian
A new carbazole-azine based fluorescent sensor was synthesized and characterized. The selectivity of the sensor for Cu(2+) over other counter ions in a dimethyl sulfoxide/H2 O mixture was shown through enhancement in fluorescence - an off to on transformation. The specificity of the probe towards Cu(2+) was evident in ultraviolet/visible, fluorescence, Fourier transform infrared and mass studies. Application of the probe in the cell imaging and cytotoxicity of living cells is illustrated.
June 20, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28630487/multicolor-two-photon-imaging-of-endogenous-fluorophores-in-living-tissues-by-wavelength-mixing
#11
Chiara Stringari, Lamiae Abdeladim, Guy Malkinson, Pierre Mahou, Xavier Solinas, Isabelle Lamarre, Sébastien Brizion, Jean-Baptiste Galey, Willy Supatto, Renaud Legouis, Ana-Maria Pena, Emmanuel Beaurepaire
Two-photon imaging of endogenous fluorescence can provide physiological and metabolic information from intact tissues. However, simultaneous imaging of multiple intrinsic fluorophores, such as nicotinamide adenine dinucleotide(phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD) and retinoids in living systems is generally hampered by sequential multi-wavelength excitation resulting in motion artifacts. Here, we report on efficient and simultaneous multicolor two-photon excitation of endogenous fluorophores with absorption spectra spanning the 750-1040 nm range, using wavelength mixing...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28630436/a-novel-gd-dtpa-conjugated-poly-l-%C3%AE-glutamyl-glutamine-paclitaxel-polymeric-delivery-system-for-tumor-theranostics
#12
Lipeng Gao, Jinge Zhou, Jing Yu, Qilong Li, Xueying Liu, Lei Sun, Ting Peng, Jing Wang, Jianzhong Zhu, Jihong Sun, Weiyue Lu, Lei Yu, Zhiqiang Yan, Yiting Wang
The conventional chemotherapeutics could not be traced in vivo and provide timely feedback on the clinical effectiveness of drugs. In this study, poly(L-γ-glutamyl-glutamine)-paclitaxel (PGG-PTX), as a model polymer, was chemically conjugated with Gd-DTPA (Gd-diethylenetriaminepentaacetic acid), a T1-contrast agent of MRI, to prepare a Gd-DTPA-conjugated PGG-PTX (PGG-PTX-DTPA-Gd) delivery system used for tumor theranostics. PGG-PTX-DTPA-Gd can be self-assembled to NPs in water with a z-average hydrodynamic diameter about 35...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28630286/efficient-switching-of-mcherry-fluorescence-using-chemical-caging
#13
Bas M C Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C Kapitein
Fluorophores with dynamic or controllable fluorescence emission have become essential tools for advanced imaging, such as superresolution imaging. These applications have driven the continuing development of photoactivatable or photoconvertible labels, including genetically encoded fluorescent proteins. These new probes work well but require the introduction of new labels that may interfere with the proper functioning of existing constructs and therefore require extensive functional characterization. In this work we show that the widely used red fluorescent protein mCherry can be brought to a purely chemically induced blue-fluorescent state by incubation with β-mercaptoethanol (βME)...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28629611/sentinel-lymph-node-mapping-in-minimally-invasive-surgery-role-of-imaging-with-color-segmented-fluorescence-csf
#14
Maite I Lopez Labrousse, Michael Frumovitz, M Guadalupe Patrono, Pedro T Ramirez
OBJECTIVE: Sentinel lymph node mapping, alone or in combination with pelvic lymphadenectomy, is considered a standard approach in staging of patients with cervical or endometrial cancer [1-3]. The goal of this video is to demonstrate the use of indocyanine green (ICG) and color-segmented fluorescence when performing lymphatic mapping in patients with gynecologic malignancies. METHODS: Injection of ICG is performed in two cervical sites using 1mL (0.5mL superficial and deep, respectively) at the 3 and 9 o'clock position...
June 16, 2017: Gynecologic Oncology
https://www.readbyqxmd.com/read/28629057/a-facile-strategy-for-fabrication-of-aggregation-induced-emission-aie-active-fluorescent-polymeric-nanoparticles-fpns-via-post-modification-of-synthetic-polymers-and-their-cell-imaging
#15
Yanzhu Liu, Liucheng Mao, Xinhua Liu, Meiying Liu, Dazhuang Xu, Ruming Jiang, Fengjie Deng, Yongxiu Li, Xiaoyong Zhang, Yen Wei
Aggregation-induced emission (AIE) active fluorescent polymeric nanoparticles (FPNs) have recently emerged as the promising nanoprobes for biological imaging for their intensive fluorescence, good photostability, desirable biocompatibility and well designability of structure and optical properties. Herein, we proposed a novel strategy for fabrication of AIE-active FPNs through the post modification of synthetic copolymers to form Schiff base. The size, morphology, optical properties and biocompatibility as well as cell uptake behavior were evaluated in detailed...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629054/synthesis-and-cell-imaging-applications-of-amphiphilic-aie-active-poly-amino-acid-s
#16
Jianwen Tian, Ruming Jiang, Peng Gao, Dazhuang Xu, Liucheng Mao, Guangjian Zeng, Meiying Liu, Fengjie Deng, Xiaoyong Zhang, Yen Wei
The poly(amino acid)s based biomaterials have attracted great research attention over the past few decades because of their biocompatibility, biodegradability and well designability. Although much progress has achieved in the synthesis and biomedical applications of poly(amino acid)s, the synthesis of luminescent poly(amino acid)s has been rarely reported. In this work, novel amphiphilic luminescent poly(amino acid)s with aggregation-induced emission (AIE) feature have been synthesized by a new approach of controlling N-carboxy anhydride (NCA) ring-opening polymerization, in which hydrophobic 2-(4-aminophenyl)-3-(10-hexadecyl-4H-phenothiazin-3-yl)acrylonitrile (Phe-NH2) with AIE feature was used as initiator and hydrophilic oligomeric glycol functionalized glutamate (OEG-glu) NCA was acted as monomer...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629043/carbon-quantum-dots-from-carbonized-walnut-shells-structural-evolution-fluorescence-characteristics-and-intracellular-bioimaging
#17
Chaoge Cheng, Yanni Shi, Min Li, Malcolm Xing, Qilin Wu
Carbon quantum dots (CQDs) have attracted tremendous attention for their prominent fluorescence, excellent stability, and outstanding biocompatibility. Herein, we report a facile method to prepare CQDs from walnut shells, which mainly consist of natural cellulose. After carbonization of the walnut shells and acid treatments, zigzag and armchair edges of CQDs with an average size of 3.4nm were revealed by the high-resolution transmission electron microscopy. Consistent with the (100) planes of graphitic carbon, the lattice spacing distance of these CQDs was 0...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28629007/upconverting-and-persistent-luminescent-nanocarriers-for-accurately-imaging-guided-photothermal-therapy
#18
Peiqi Zhao, Wei Ji, Shiyong Zhou, Lihua Qiu, Lanfang Li, Zhengzi Qian, Xianming Liu, Huilai Zhang, Xuchen Cao
The fluorescence-guided photothermal therapy (FPTT) has great potential in cancer treatment. However, the conventional FPTT has to be stimulated by external light, which tends to increase background noise and leads to the inaccurate infrared light irradiation for PTT. In this study, upconverting and persistent luminescent nanocarriers (UPLNs) loaded mesoporous silica nanoparticles (UPLNs@mSiO2) were first designed to solve the problem mentioned above. The UPLNs cores can effectively reduce the short-lived autofluorescence interference by exerting the delay time between signal acquisition and pulsed excitation light...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28628728/characterizing-the-detection-threshold-for-optical-imaging-in-surgical-oncology
#19
Andrew C Prince, Aditi Jani, Melissa Korb, Kiranya E Tipirneni, Benjamin B Kasten, Eben L Rosenthal, Jason M Warram
BACKGROUND AND OBJECTIVES: Optical imaging to guide cancer resections is rapidly transitioning into the operating room. However, the sensitivity of this technique to detect subclinical disease is yet characterized. The purpose of this study was to determine the minimum range of cancer cells that can be detected by antibody-based fluorescence imaging. METHODS: 2LMP (breast), COLO-205 (colon), MiaPaca-2 (pancreas), and SCC-1 (head and neck) cells incubated in vitro with cetuximab-IRDye800CW (dose range 8...
June 19, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28628674/hepatic-microvascular-dysfunction-and-increased-advanced-glycation-end-products-are-components-of-non-alcoholic-fatty-liver-disease
#20
Evelyn Nunes Goulart da Silva Pereira, Raquel Rangel Silvares, Edgar Eduardo Ilaquita Flores, Karine Lino Rodrigues, Isalira Peroba Ramos, Igor José da Silva, Marcelo Pelajo Machado, Rosiane Aparecida Miranda, Carmen Cabanelas Pazos-Moura, Cassiano F Gonçalves-de-Albuquerque, Hugo Caire de Castro Faria-Neto, Eduardo Tibiriça, Anissa Daliry
BACKGROUND: This study aimed to investigate the pathophysiology of hepatic microcirculatory dysfunction in non-alcoholic fatty liver disease (NAFLD). METHODS: In Wistar rats, NAFLD model was induced by 20 weeks of high-fat diet (HFD) feeding. Rolling and adhesion of leukocytes and tissue perfusion in hepatic microcirculation were examined using in vivo microscopic and laser speckle contrast imaging (LSCI), respectively. Oxidative stress and inflamatory parameters were analysed by TBARs, catalase enzyme activity, RT-PCR and ELISA...
2017: PloS One
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