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https://www.readbyqxmd.com/read/28328544/non-toxic-fluorescent-phosphonium-probes-to-detect-mitochondrial-potential
#1
Ana Šarić, Ivo Crnolatac, Frédéric Bouillaud, Sandra Sobočanec, Ana-Matea Mikecin, Željka Mačak Šafranko, Todor Delgeorgiev, Ivo Piantanida, Tihomir Balog, Patrice X Petit
We evaluated our phosphonium-based fluorescent probes for selective staining of mitochondria. Currently used probes for monitoring mitochondrial membrane potential show varying degrees of interference with cell metabolism, photo-induced damage and probe binding. Here presented probes are characterised by highly efficient cellular uptake and specific accumulation in mitochondria. Fluorescent detection of the probes was accomplished using flow cytometry and confocal microscopy imaging of yeast and mammalian cells...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328542/an-excited-state-intramolecular-photon-transfer-fluorescence-probe-for-localizable-live-cell-imaging-of-cysteine
#2
Wei Liu, Wen Chen, Si-Jia Liu, Jian-Hui Jiang
Small molecule probes suitable for selective and specific fluorescence imaging of some important but low-concentration intracellular reactive sulfur species such as cysteine (Cys) pose a challenge in chemical biology. We present a readily available, fast-response fluorescence probe CHCQ-Ac, with 2-(5'-chloro-2-hydroxyl-phenyl)-6-chloro-4(3 H)-quinazolinone (CHCQ) as the fluorophore and acrylate group as the functional moiety, that enables high-selectivity and high-sensitivity for detecting Cys in both solution and biological system...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328541/molecular-engineering-of-two-photon-fluorescent-probes-for-bioimaging-applications
#3
Hong-Wen Liu, Yongchao Liu, Peng Wang, Xiao-Bing Zhang
During the past two decades, two-photon microscopy (TPM), which utilizes two near-infrared photons as the excitation source, has emerged as a novel, attractive imaging tool for biological research. Compared with one-photon microscopy, TPM offers several advantages, such as lowering background fluorescence in living cells and tissues, reducing photodamage to biosamples, and a photobleaching phenomenon, offering better 3D spatial localization, and increasing penetration depth. Small-molecule-based two-photon fluorescent probes have been well developed for the detection and imaging of various analytes in biological systems...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328539/polarization-sensitive-localization-based-super-resolution-microscopy-with-a-birefringent-wedge
#4
József Sinkó, Tamás Gajdos, Elvira Czvik, Gábor Szabó, Miklós Erdélyi
A practical method has been presented for polarization sensitive localization based super-resolution microscopy using a birefringent dual wedge. The measurement of the polarization degree at the single molecule level can reveal the chemical and physical properties of the local environment of the fluorescent dye molecule and can hence provide information about the sub-diffraction sized structure of biological samples. Polarization sensitive STORM imaging of the F-Actins proved correlation between the orientation of fluorescent dipoles and the axis of the fibril...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328502/spatial-statistics-for-segmenting-histological-structures-in-h-e-stained-tissue-images
#5
Luong Nguyen, A Burak Tosun, Jeffrey Fine, Adrian Lee, D Lansing Taylor, Chakra Chennubhotla
Segmenting a broad class of histological structures in transmitted light and/or fluorescence-based images is a prerequisite for determining the pathological basis of cancer, elucidating spatial interactions between histological structures in tumor microenvironments (e.g. tumor infiltrating lymphocytes), facilitating precision medicine studies with deep molecular profiling, and providing an exploratory tool for pathologists. Our paper focuses on segmenting histological structures in hematoxylin and eosin (H&E) stained images of breast tissues, e...
March 16, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28328200/fluorescence-resonance-energy-transfer-based-dna-tetrahedron-nano-tweezer-for-highly-reliable-detection-of-tumor-related-mrna-in-living-cells
#6
Lei He, Dan-Qing Lu, Hao Liang, Sitao Xie, Can Luo, Miaomiao Hu, Liujun Xu, Xiaobing Zhang, Weihong Tan
Accurate detection and imaging of tumor-related mRNA in living cells hold great promise for early cancer detection. However, currently, most probes designed to image intracellular mRNA confront intrinsic interferences arising from complex biological matrices and resulting in inevitable false-positive signals. To circumvent this problem, a new class of intracellular DNA nanoprobe, termed DNA tetrahedron nano-tweezer (DTNT), was developed to reliably image tumor-related mRNA in living cells based on the FRET (fluorescence resonance energy transfer) "off" to "on" signal readout mode...
March 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28328030/estimation-of-microscope-drift-using-fluorescent-nanodiamonds-as-fiducial-markers
#7
W Colomb, J Czerski, J D Sau, S K Sarkar
Fiducial markers are used to correct the microscope drift and should be photostable, be usable at multiple wavelengths and be compatible for multimodal imaging. Fiducial markers such as beads, gold nanoparticles, microfabricated patterns and organic fluorophores lack one or more of these criteria. Moreover, the localization accuracy and drift correction can be degraded by other fluorophores, instrument noise and artefacts due to image processing and tracking algorithms. Estimating mechanical drift by assuming Gaussian distributed noise is not suitable under these circumstances...
March 22, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28328028/large-area-elemental-imaging-reveals-van-eyck-s-original-paint-layers-on-the-ghent-altarpiece-1432-rescoping-its-conservation-treatment
#8
Geert Van der Snickt, Hélène Dubois, Jana Sanyova, Stijn Legrand, Alexia Coudray, Cécile Glaude, Marie Postec, Piet Van Espen, Koen Janssens
A combination of large-scale and micro-scale elemental imaging, yielding elemental distribution maps obtained by, respectively non-invasive macroscopic X-ray fluorescence (MA-XRF) and by secondary electron microscopy/energy dispersive X-ray analysis (SEM-EDX) and synchrotron radiation-based micro-XRF (SR μ-XRF) imaging was employed to reorient and optimize the conservation strategy of van Eyck's renowned Ghent Altarpiece. By exploiting the penetrative properties of X-rays together with the elemental specificity offered by XRF, it was possible to visualize the original paint layers by van Eyck hidden below the overpainted surface and to simultaneously assess their condition...
March 22, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28327978/a-dataset-of-images-and-morphological-profiles-of-30-000-small-molecule-treatments-using-the-cell-painting-assay
#9
Mark-Anthony Bray, Sigrun M Gustafsdottir, Vebjorn Ljosa, Shantanu Singh, Katherine L Sokolnicki, Joshua A Bittker, Nicole E Bodycombe, Vlado Dancík, Thomas P Hasaka, C Suk-Yee Hon, Melissa M Kemp, Kejie Li, Deepika Walpita, Mathias J Wawer, Todd R Golub, Stuart L Schreiber, Paul A Clemons, Alykhan F Shamji, Anne E Carpenter
Background: Large-scale image sets acquired by automated microscopy of perturbed samples enable a detailed comparison of cell states induced by each perturbation, such as a small molecule from a diverse library. Highly multiplexed measurements of cellular morphology can be extracted from each image and subsequently mined for a number of applications. Findings: This microscopy data set includes 919,874 five-channel fields of view representing 30,616 tested compounds, available at 'The Cell Image Library' repository...
January 7, 2017: GigaScience
https://www.readbyqxmd.com/read/28327735/reverse-micelle-based-water-soluble-nanoparticles-for-simultaneous-bioimaging-and-drug-delivery
#10
Ying Chen, Yong Liu, Yongchao Yao, Shiyong Zhang, Zhongwei Gu
With special confined water pools, reverse micelles (RMs) have shown potential for a wide range of applications. However, the inherent water-insolubility of RMs hinders their further application prospects, especially for applications related to biology. We recently reported the first successful transfer of RMs from organic media to an aqueous phase without changing the smart water pools by the hydrolysis of an arm-cleavable interfacial cross-linked reverse micelles. Herein, we employed another elaborate amphiphile 1 to construct new acrylamide-based cross-linked water-soluble nanoparticles (ACW-NPs) under much gentler conditions...
March 22, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28327707/direct-visualization-of-carbon-nanotube-degradation-in-primary-cells-by-photothermal-imaging
#11
Julie Russier, Laura Oudjedi, Martin Piponnier, Cyrill Bussy, Maurizio Prato, Kostas Kostarelos, Brahim Lounis, Alberto Bianco, Laurent Cognet
Assessment of biodegradability of carbon nanotubes (CNTs) is a critically important aspect that needs to be solved before their translation into new biomedical tools. CNT biodegradation has been shown both in vitro and in vivo, but we are limited by the number of analytical techniques that can be used to follow the entire process. Photothermal imaging (PhI) is an innovative technique that enables the quantitative detection of nanometer-sized absorptive objects. In this study, we demonstrate that PhI allows the observation of the degradation process of functionalized multi-walled carbon nanotubes (MWCNTs) following their internalization by primary glial cells...
March 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/28327582/real-time-sentinel-lymph-node-biopsy-guidance-using-combined-ultrasound-photoacoustic-fluorescence-imaging-in-vivo-proof-of-principle-and-validation-with-nodal-obstruction
#12
Jeeun Kang, Jin Ho Chang, Sun Mi Kim, Hak Jong Lee, Haemin Kim, Brian C Wilson, Tai-Kyong Song
Precise sentinel lymph node (SLN) identification is crucial not only for accurate diagnosis of micro-metastases at an early stage of cancer progression but also for reducing the number of SLN biopsies (SLNB) to minimize their severe side effects. Furthermore, it is desirable that an SLNB guidance should be as safe as possible in routine clinical use. Although there are currently various SLNB guidance methods for pre-operative or intra-operative assessment, none are ideal. We propose a real-time SLNB guidance method using contrast-enhanced tri-modal images (i...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28326794/urothelial-tumors-and-dual-band-imaging-a-new-concept-in-confocal-laser-endomicroscopy
#13
Arnaud Marien, Aurélien Rock, Khaled El Maadarani, Catherine Francois, Pierre Gosset, Brigitte Mauroy, Jean-Louis Bonnal
OBJECTIVES: Confocal laser endomicroscopy (CLE) uses a low-energy laser light source to obtain microscopic histology images of bladder tissue exposed to a fluorescent dye. To evaluate the feasibility of using CLE with two fluorophores: fluorescein (FLUO) and hexylaminolevulinate (HAL) to determine histologic and cytologic bladder cancer criteria. METHODS: Patients eligible for HAL-photodynamic diagnosis-assisted transurethral resection of bladder tumor were included...
March 22, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28326459/preparation-and-evaluation-of-plga-coated-capsaicin-magnetic-nanoparticles
#14
Mrudhula Baskaran, Padmamalini Baskaran, Navamoney Arulsamy, Baskaran Thyagarajan
PURPOSE: Drugs used in the treatment of diseases can cause several unwanted systemic side effects. A site-specific drug delivery system can eliminate such consequences by delivering drugs to certain target areas of the body where therapeutic effects are required. Here we present the preparation and evaluation of magnetic nanoparticles of capsaicin, the active ingredient in chili peppers, coated with poly-L-lactide co-glycolide (PLGA), a FDA-approved biodegradable bioavailable polymer...
March 21, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28325928/a-microplate-reader-based-system-for-visualizing-transcriptional-activity-during-in-vivo-microbial-interactions-in-space-and-time
#15
Rosanna C Hennessy, Peter Stougaard, Stefan Olsson
Here, we report the development of a microplate reader-based system for visualizing gene expression dynamics in living bacterial cells in response to a fungus in space and real-time. A bacterium expressing the red fluorescent protein mCherry fused to the promoter region of a regulator gene nunF indicating activation of an antifungal secondary metabolite gene cluster was used as a reporter system. Time-lapse image recordings of the reporter red signal and a green signal from fluorescent metabolites combined with microbial growth measurements showed that nunF-regulated gene transcription is switched on when the bacterium enters the deceleration growth phase and upon physical encounter with fungal hyphae...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28325018/imquant-an-image-based-fluorescence-reader-for-quantitative-lateral-flow-immunoassays
#16
Kaushik Basak Chowdhury, Jayaraj Joseph, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam
Fluorescence immunoassays (FIA) have strong potential in enabling rapid quantitative immunological testing at point of care. Existing state of the art instruments employ laser scanning of FIA test kits, which require the use of electromechanical systems, making the instrument bulky, heavy and less reliable in the long term. There is a growing need for an extremely compact and reliable, motion-free, automated reader for accurate quantification of immunoassays. Here, we present ImQuant, an image based FIA reader with integrated monochromatic excitation source, optical filtering and real time image capture, assisted with an intuitive graphical user interface...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324863/croconaine-nanoparticles-with-enhanced-tumor-accumulation-for-multimodality-cancer-theranostics
#17
Longguang Tang, Fuwu Zhang, Fei Yu, Wenjing Sun, Menglin Song, Xiaoyuan Chen, Xianzhong Zhang, Xiaolian Sun
A novel nanoparticle self-assembled by polyethylene glycol (PEG) modified croconaine dye (CR780) is presented for photoacoustic (PA)/near-infrared (NIR) fluorescence imaging-guided photothermal therapy (PTT). The simple PEGylation made CR780 amphiphilic, and led to their self-assembly into well-defined and uniform nanostructures with size tunable by controlling the assembly conditions. The CR780-PEG5K not only displayed the strength of small molecules (including rapid distribution to different organs, fast renal clearance and minimal accumulation to normal tissues), but also demonstrated the advantages of nanomaterials (including high physiological stability, multimodal theranostic ability, high tumor accumulation and retention)...
March 13, 2017: Biomaterials
https://www.readbyqxmd.com/read/28324723/specific-light-up-pullulan-based-nanoparticles-with-reduction-triggered-emission-and-activatable-photoactivity-for-the-imaging-and-photodynamic-killing-of-cancer-cells
#18
Jing Xia, Liuwei Zhang, Ming Qian, Yongming Bao, Jingyun Wang, Yachen Li
Activatable photosensitizers that can be activated by cancer-associated stimuli have drawn increasing attention for simultaneous fluorescence imaging and photodynamic ablation of cancer cells. Here, we developed a cancer-cell specific photosensitizer nano-delivery system by synthesizing protoporphyrin IX (PpIX)-conjugated pullulan (P) with reducible disulfide bonds. The amphiphilic P-s-s-PpIX conjugate self-assembled in aqueous condition to form core-shell structured nanoparticles (P-s-s-PpIX NPs) with average size of 166nm, showing reduction-controllable stability...
March 15, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28324684/tea-is-a-key-virulence-factor-for-alternaria-alternata-fr-keissler-infection-of-its-host
#19
Ye Kang, Hongwei Feng, Jingxu Zhang, Shiguo Chen, Bernal E Valverde, Sheng Qiang
A toxin-deficient mutant strain, HP001 mutant of Alternaria alternata, whose mycelium is unable to infect its host, produces little tenuazonic acid (TeA) toxin. How TeA plays a role in initiating host infection by A. alternata remains unclear. In this research we use Imaging-PAM based on chlorophyll fluorescence parameters and transmission electron microscopy to explore the role of TeA toxin during the infection process of A. alternata. Photosystem II damage began even before wild type mycelium infected the leaves of its host, croftonweed (Ageratina adenophora)...
March 4, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28324614/fluorescence-based-high-throughput-and-targeted-image-acquisition-and-analysis-for-phenotypic-screening
#20
Manuel Gunkel, Jan Philipp Eberle, Holger Erfle
Applying the right acquisition method in a fluorescence imaging-based screening context is of great importance to obtain an appropriate readout and to select the right scale of the screen. In order to save imaging time and data, we have developed routines for multiscale targeted imaging, providing both a broad overview of a sample and additional in-depth information for targets of interest identified within the screen. These objects can be identified and acquired on-the-fly by an interconnection of image acquisition and image analysis...
2017: Methods in Molecular Biology
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