keyword
MENU ▼
Read by QxMD icon Read
search

Fluorescent imaging

keyword
https://www.readbyqxmd.com/read/27915091/electroconvulsive-stimulation-transiently-enhances-the-permeability-of-the-rat-blood-brain-barrier-and-induces-astrocytic-changes
#1
Masanobu Ito, Kuerban Bolati, Tomoya Kinjo, Koichiro Ichimura, Akiko Furuta, Declan M McLoughlin, Toshihito Suzuki, Heii Arai
The blood-brain barrier (BBB) plays important roles in both the physiological and pharmacological state of the brain. Transiently enhancing the permeability of the BBB may allow use of more types of medications for neuropsychiatric diseases. Several studies have demonstrated that seizures cause a transient decrease in BBB integrity. We studied the timing of BBB changes following seizures and the role of astrocytes in this process. Rats received 10 applications of electroconvulsive stimulation (ECS). They were then infused with sodium fluorescein, a fluorescent substance that rarely passes the BBB, via the inferior vena cava...
November 30, 2016: Brain Research Bulletin
https://www.readbyqxmd.com/read/27914988/imaging-of-macrophage-dynamics-with-optical-coherence-tomography-in-anterior-ischemic-optic-neuropathy
#2
Despina Kokona, Nathanael U Häner, Andreas Ebneter, Martin S Zinkernagel
Anterior ischemic optic neuropathy (AION) is a relatively common cause of visual loss and results from hypoperfusion of the small arteries of the anterior portion of the optic nerve. AION is the leading cause of sudden optic nerve related vision loss with approximately 10 cases per 100'000 in the population over 50 years. To date there is no established treatment for AION and therefore a better understanding of the events occurring at the level of the optic nerve head (ONH) would be important to design future therapeutic strategies...
November 30, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27914984/recapitulating-the-human-tumor-microenvironment-colon-tumor-derived-extracellular-matrix-promotes-angiogenesis-and-tumor-cell-growth
#3
Mónica Romero-López, Andrew L Trinh, Agua Sobrino, Michaela M S Hatch, Mark T Keating, Cristhian Fimbres, David E Lewis, Paul D Gershon, Elliot L Botvinick, Michelle Digman, John S Lowengrub, Christopher C W Hughes
Extracellular matrix (ECM) is an essential and dynamic component of all tissues and directly affects cellular behavior by providing both mechanical and biochemical signaling cues. Changes in ECM can alter tissue homeostasis, potentially leading to promotion of cellular transformation and the generation of tumors. Therefore, understanding ECM compositional changes during cancer progression is vital to the development of targeted treatments. Previous efforts to reproduce the native 3D cellular microenvironment have utilized protein gels and scaffolds that incompletely recapitulate the complexity of native tissues...
November 24, 2016: Biomaterials
https://www.readbyqxmd.com/read/27914983/neuropeptide-y-y1-receptor-mediated-biodegradable-photoluminescent-nanobubbles-as-ultrasound-contrast-agents-for-targeted-breast-cancer-imaging
#4
Juan Li, Yuchen Tian, Dingying Shan, An Gong, Leyong Zeng, Wenzhi Ren, Lingchao Xiang, Ethan Gerhard, Jinshun Zhao, Jian Yang, Aiguo Wu
Targeted molecular imaging has attracted great attention in cancer diagnosis and treatment. However, most clinically used ultrasound contrast agents (UCAs) are non-targeted microbubbles seldom used for cancer imaging. Here, we fabricated fluorescent nanobubbles (NBs) by encapsulation of liquid tetradecafluorohexane (C6F14) within biodegradable photoluminescent polymers (BPLPs) through an emulsion-evaporation process and conjugation of PNBL-NPY ligand for specific targeting of Y1 receptors overexpressed in breast tumors...
November 22, 2016: Biomaterials
https://www.readbyqxmd.com/read/27914952/independent-tonotopy-and-thalamocortical-projection-patterns-in-two-adjacent-parts-of-the-classical-primary-auditory-cortex-in-mice
#5
Hiroaki Tsukano, Masao Horie, Kuniyuki Takahashi, Ryuichi Hishida, Hirohide Takebayashi, Katsuei Shibuki
Amid recent amendment of delineation of a mouse auditory cortical map, a caudal auditory field, originally defined as the primary auditory cortex (AI), was divided into the AI and dorsomedial field (DM), based on distinct high frequency areas. A low frequency area was not previously established in the DM because responses to low frequency tones were weak in this area. This may lead to the misconception that the DM is an atypical region that lacks a low frequency band. In the current study, we confirmed that the DM has a low frequency area that is completely independent from the AI...
November 30, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/27914343/fluorescence-and-drug-loading-properties-of-znse-mn-zns-paclitaxel-sio2-nanocapsules-templated-by-f127-micelles
#6
Tingting Zhao, Xin Liu, Yuebin Li, Mingguang Zhang, Jian He, Xuewen Zhang, Hui Liu, Xingguo Wang, Haoshuang Gu
Hydrophobic ZnSe:Mn/ZnS core-shell fluorescence quantum dots (QDs) and anticancer drug paclitaxel (PTX) have been co-loaded into folate conjugated hybrid silica nanocapsules via F127 micelles based soft-template method in a mild aqueous environment at room temperature. The encapsulation of QDs shows a F127: QDs mass ratio dependent behavior, which impact much on the morphology and optical properties of composite nanocapsules. These as prepared composite nanocapsules also exhibit good photoluminescence stability under the temperature ranges from 19°C to 49°C...
November 23, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27914328/synthesis-and-stability-of-ir-820-and-fitc-doped-silica-nanoparticles
#7
Atul V Thorat, Tandra Ghoshal, Lan Chen, Justin D Holmes, Michael A Morris
Fluorescent silica nanoparticles (NPs) have potential in biomedical applications as diagnostics and traceable drug delivery agents. In this study, we have synthesized fluorescent dye grafted silica NPs in two step process. First, a stable method to synthesize various sizes of silica NPs ranging from 20 to 52, 95, 210 and 410nm have been successfully demonstrated. Secondly, as-synthesized silica NPs are readily grafted with some fluorescent dyes like IR-820 and fluorescein isothiocyanate (FITC) dyes by simple impregnation method...
November 15, 2016: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/27914080/immobilization-techniques-of-bacteria-for-live-super-resolution-imaging-using-structured-illumination-microscopy
#8
Amy L Bottomley, Lynne Turnbull, Cynthia B Whitchurch, Elizabeth J Harry
Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-quality microscopy is good sample preparation to avoid bacterial cell movement that can result in motion blur during image acquisition. Here, we describe two techniques of sample preparation that reduce unwanted cell movement and are suitable for application to a number of bacterial species and imaging methods...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27914077/making-fluorescent-streptococci-and-enterococci-for-live-imaging
#9
Sarah Shabayek, Barbara Spellerberg
Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, outstanding fluorescent labeling tools with numerous applications in vastly different areas of life sciences have been developed. To optimize GFP for diverse life science applications, a large variety of GFP derivatives with different environmental characteristics have been generated by mutagenesis. The enhanced green fluorescent protein (EGFP) is a well-known GFP derivative with highly increased fluorescence intensity compared to the GFP wild-type molecule...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27913947/preclinical-evaluation-of-cathepsin-based-fluorescent-imaging-system-for-cytoreductive-surgery
#10
Carlos H F Chan, Lukas F Liesenfeld, Isabel Ferreiro-Neira, James C Cusack
BACKGROUND: Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy (CRS-HIPEC) is a treatment option for peritoneal surface malignancies. The ability to detect microscopic foci of peritoneal metastasis intraoperatively may ensure the completeness of cytoreduction. In this study, we evaluated the suitability of a hand-held cathepsin-based fluorescent imaging system for intraoperative detection of appendiceal and colorectal peritoneal metastasis. METHODS: Peritoneal tumors and normal peritoneal tissues were collected from patients with appendiceal and colorectal peritoneal metastasis...
December 2, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27913845/mitogen-activated-protein-kinases-are-involved-in-hepatocanalicular-dysfunction-and-cholestasis-induced-by-oxidative-stress
#11
Flavia D Toledo, Cecilia L Basiglio, Ismael R Barosso, Andrea C Boaglio, Andrés E Zucchetti, Enrique J Sánchez Pozzi, Marcelo G Roma
In previous studies, we showed that the pro-oxidant model agent tert-butyl hydroperoxide (tBuOOH) induces alterations in hepatocanalicular secretory function by activating Ca(2+)-dependent protein kinase C isoforms (cPKC), via F-actin disorganization followed by endocytic internalization of canalicular transporters relevant to bile formation (Mrp2, Bsep). Since mitogen-activated protein kinases (MAPKs) may be downstream effectors of cPKC, we investigated here the involvement of the MAPKs of the ERK1/2, JNK1/2, and p38(MAPK) types in these deleterious effects...
December 2, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27912872/anti-allergic-potential-of-typhonium-blumei-inhibition-of-degranulation-via-suppression-of-pi3k-plc%C3%AE-2-phosphorylation-and-calcium-influx
#12
Michal Korinek, Kuan-Ming Chen, Yu-Han Jiang, Mohamed El-Shazly, Joel Stocker, Chon-Kit Chou, Tsong-Long Hwang, Yang-Chang Wu, Bing-Hung Chen, Fang-Rong Chang
BACKGROUND: Typhonium blumei Nicolson & Sivadasan (Araceae) is a traditional Chinese medicinal herb possessing detumescent, detoxifying, and anti-inflammatory activities. It is used in Taiwan as a folk medicine to treat cancer and inflammatory diseases. Typhonium blumei is usually not distinguished from Typhonium roxburghii Schott and they are commonly used interchangeably. PURPOSE: To evaluate and compare the anti-allergic and anti-inflammatory properties of T...
December 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27911982/enantioselective-fluorescent-imaging-of-free-amino-acids-in-living-cells
#13
Chaoyuan Zeng, Xiaoling Zhang, Lin Pu
The 1,1'-spirobiindane-7,7'-diol (SPINOL)-based chiral aldehydes (R)- and (S)-3 in combination with Zn2+ are found to be highly enantioselective fluorescent sensors for structurally diverse chiral amino acids. Application of this enantiomeric sensor pair in HeLa cells containing a free amino acid shows that the chirality matched sensor-amino acid interaction leads to strong blue emission and the chirality mismatched gives only weak fluorescence. This allows a quick determination of the chiral configuration of the free amino acid in living cells...
December 2, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27911841/high-performance-multiplexed-fluorescence-in-situ-hybridization-in-culture-and-tissue-with-matrix-imprinting-and-clearing
#14
Jeffrey R Moffitt, Junjie Hao, Dhananjay Bambah-Mukku, Tian Lu, Catherine Dulac, Xiaowei Zhuang
Highly multiplexed single-molecule FISH has emerged as a promising approach to spatially resolved single-cell transcriptomics because of its ability to directly image and profile numerous RNA species in their native cellular context. However, background-from off-target binding of FISH probes and cellular autofluorescence-can become limiting in a number of important applications, such as increasing the degree of multiplexing, imaging shorter RNAs, and imaging tissue samples. Here, we developed a sample clearing approach for FISH measurements...
November 22, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27911813/spatiotemporal-imaging-of-small-gtpases-activity-in-live-cells
#15
Stephanie Voss, Dennis M Krüger, Oliver Koch, Yao-Wen Wu
Ras-like small GTPases function as molecular switches and regulate diverse cellular events. To examine the dynamics of signaling requires spatiotemporal visualization of their activity in the cell. Current small GTPase sensors rely on specific effector domains that are available for only a small number of GTPases and compete for endogenous regulator/effector binding. Here, we describe versatile conformational sensors for GTPase activity (COSGAs) based on the conserved GTPase fold. Conformational changes upon GDP/GTP exchange were directly observed in solution, on beads, and in live cells by Förster resonance energy transfer (FRET)...
November 29, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27911379/rapid-acquisition-of-3d-images-using-high-resolution-episcopic-microscopy
#16
Haochuan Zhang, JunGang Huang, Xin Liu, Ping Zhu, Zhongrong Li, Xue Li
High-resolution episcopic microscopic (HREM) technology enables rapid acquisition of high-resolution digital volumetric and three-dimensional (3D) morphometric data. Here, we describe the detailed protocol to image the entire mouse embryo. The protocol consists of four major sections: sample preparation, embedding, image acquisition and finally, 3D visualization. The technology requires specimens to be stained with a fluorescent dye, which can be problematic for large or dense specimens. To overcome this limitation, we have improved the existing protocol to enhance tissue penetration of the dye by pretreating the specimen with a solution containing urea and sodium dodecyl sulfate...
November 21, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910894/calcium-ions-function-as-a-booster-of-chromosome-condensation
#17
Rinyaporn Phengchat, Hideaki Takata, Kenichi Morii, Noriko Inada, Hideji Murakoshi, Susumu Uchiyama, Kiichi Fukui
Chromosome condensation is essential for the faithful transmission of genetic information to daughter cells during cell division. The depletion of chromosome scaffold proteins does not prevent chromosome condensation despite structural defects. This suggests that other factors contribute to condensation. Here we investigated the contribution of divalent cations, particularly Ca(2+), to chromosome condensation in vitro and in vivo. Ca(2+) depletion caused defects in proper mitotic progression, particularly in chromosome condensation after the breakdown of the nuclear envelope...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910868/relationship-between-nanotopographical-alignment-and-stem-cell-fate-with-live-imaging-and-shape-analysis
#18
Peter Newman, Jorge Luis Galenano-Niño, Pamela Graney, Joselito M Razal, Andrew I Minett, João Ribas, Raquel Ovalle-Robles, Maté Biro, Hala Zreiqat
The topography of a biomaterial regulates cellular interactions and determine stem cell fate. A complete understanding of how topographical properties affect cell behavior will allow the rational design of material surfaces that elicit specified biological functions once placed in the body. To this end, we fabricate substrates with aligned or randomly organized fibrous nanostructured topographies. Culturing adipose-derived stem cells (ASCs), we explore the dynamic relationship between the alignment of topography, cell shape and cell differentiation to osteogenic and myogenic lineages...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910670/alignment-and-absolute-wavelength-calibration-of-imaging-bragg-spectrometers
#19
G Bertschinger, O Marchuk, R Barnsley
In the present and the next generation of fusion devices, imaging Bragg spectrometers are key diagnostics to measure plasma parameters in the hot core, especially ion temperature and plasma rotation. The latter quantities are routinely obtained using the Doppler-width and -shift of the emitted spectral lines, respectively. Line shift measurements require absolute accuracies Δλ/λ of about 10 ppm, where λ-is the observed wavelength. For ITER and the present fusion devices, spectral lines of He-and H-like argon, iron, and krypton as well as Ne-like tungsten are foreseen for the measurements...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910631/a-simple-approach-to-spectrally-resolved-fluorescence-and-bright-field-microscopy-over-select-regions-of-interest
#20
Peter D Dahlberg, Christopher T Boughter, Nabil F Faruk, Lu Hong, Young Hoon Koh, Matthew A Reyer, Alon Shaiber, Aiman Sherani, Jiacheng Zhang, Justin E Jureller, Adam T Hammond
A standard wide field inverted microscope was converted to a spatially selective spectrally resolved microscope through the addition of a polarizing beam splitter, a pair of polarizers, an amplitude-mode liquid crystal-spatial light modulator, and a USB spectrometer. The instrument is capable of simultaneously imaging and acquiring spectra over user defined regions of interest. The microscope can also be operated in a bright-field mode to acquire absorption spectra of micron scale objects. The utility of the instrument is demonstrated on three different samples...
November 2016: Review of Scientific Instruments
keyword
keyword
4758
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"