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https://www.readbyqxmd.com/read/28421090/comparative-genomics-and-phylogenomics-of-east-asian-tulips-amana-liliaceae
#1
Pan Li, Rui-Sen Lu, Wu-Qin Xu, Tetsuo Ohi-Toma, Min-Qi Cai, Ying-Xiong Qiu, Kenneth M Cameron, Cheng-Xin Fu
The genus Amana Honda (Liliaceae), when it is treated as separate from Tulipa, comprises six perennial herbaceous species that are restricted to China, Japan and the Korean Peninsula. Although all six Amana species have important medicinal and horticultural uses, studies focused on species identification and molecular phylogenetics are few. Here we report the nucleotide sequences of six complete Amana chloroplast (cp) genomes. The cp genomes of Amana range from 150,613 bp to 151,136 bp in length, all including a pair of inverted repeats (25,629-25,859 bp) separated by the large single-copy (81,482-82,218 bp) and small single-copy (17,366-17,465 bp) regions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28411161/a-reassessment-of-the-phylogeny-and-circumscription-of-zaluzianskya-scrophulariaceae
#2
Jenny K Archibald, Jacqueline Cook, Bruce Anderson, Steven D Johnson, Mark E Mort
The genus Zaluzianskya (Scrophulariaceae s.s.) encompasses a diversity of traits, such as flowers ranging from 4 to 60 mm in length with various patterns of color and indumentum. However, this diversity, as described by the current taxonomic circumscription of Zaluzianskya, is an underestimate. The boundaries of this genus require expansion via incorporation of species from other genera and recognition of unnamed species. This study advances prior molecular phylogenies for the southern African genus through the addition of DNA regions and an up to 70% increase in population sampling, targeting specific taxa...
April 11, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28408750/transcriptional-regulatory-networks-underlying-the-reprogramming-of-spermatogonial-stem-cells-to-multipotent-stem-cells
#3
Hoe-Su Jeong, Jinhyuk Bhin, Hyung Joon Kim, Daehee Hwang, Dong Ryul Lee, Kye-Seong Kim
Spermatogonial stem cells (SSCs) are germline stem cells located along the basement membrane of seminiferous tubules in testes. Recently, SSCs were shown to be reprogrammed into multipotent SSCs (mSSCs). However, both the key factors and biological networks underlying this reprogramming remain elusive. Here, we present transcriptional regulatory networks (TRNs) that control cellular processes related to the SSC-to-mSSC reprogramming. Previously, we established intermediate SSCs (iSSCs) undergoing the transition to mSSCs and generated gene expression profiles of SSCs, iSSCs and mSSCs...
April 14, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28279810/madagascar-sheds-new-light-on-the-molecular-systematics-and-biogeography-of-grammitid-ferns-new-unexpected-lineages-and-numerous-long-distance-dispersal-events
#4
Lucie Bauret, Myriam Gaudeul, Michael A Sundue, Barbara S Parris, Tom A Ranker, France Rakotondrainibe, Sabine Hennequin, Jaona Ranaivo, Marc-André Selosse, Germinal Rouhan
Based on a worldwide phylogenetic framework filling the taxonomic gap of Madagascar and surrounding islands of the Western Indian Ocean (WIO), we revisited the systematics of grammitid fern species (Polypodiaceae). We also investigated the biogeographic origin of the extant diversity in Madagascar and estimated the relative influence of vicariance, long-distance dispersals (LDD) and in situ diversification. Phylogenetic inferences were based on five plastid DNA regions (atpB, rbcL, rps4-trnS, trnG-trnR, trnL-trnF) and the most comprehensive taxonomic sampling ever assembled (224 species belonging to 31 out of 33 recognized grammitids genera)...
March 6, 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28207921/pneumatic-stimulation-of-c-elegans-mechanoreceptor-neurons-in-a-microfluidic-trap
#5
Adam L Nekimken, Holger Fehlauer, Anna A Kim, Sandra N Manosalvas-Kjono, Purim Ladpli, Farah Memon, Divya Gopisetty, Veronica Sanchez, Miriam B Goodman, Beth L Pruitt, Michael Krieg
New tools for applying force to animals, tissues, and cells are critically needed in order to advance the field of mechanobiology, as few existing tools enable simultaneous imaging of tissue and cell deformation as well as cellular activity in live animals. Here, we introduce a novel microfluidic device that enables high-resolution optical imaging of cellular deformations and activity while applying precise mechanical stimuli to the surface of the worm's cuticle with a pneumatic pressure reservoir. To evaluate device performance, we compared analytical and numerical simulations conducted during the design process to empirical measurements made with fabricated devices...
March 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28188878/towards-a-higher-level-ensifera-phylogeny-inferred-from-mitogenome-sequences
#6
Zhijun Zhou, Ling Zhao, Nian Liu, Huifang Guo, Bei Guan, Juanxia Di, Fuming Shi
Although mitogenomes are useful tools for inferring evolutionary history, only a few representative ones can be used for most Ensifera lineages. Thirty-two ensiferan mitogenomes were determined using ABI Sanger sequencing and standard primer walking of 2-3 overlapping Long-PCR fragments, or Illumina® HiSeq2000 for "shotgun" sequenced long-PCR-amplified mitochondrial or total genomic DNA. Six patterns of gene arrangements, including the novel trnR-trnS(AGN)-trnA-trnN-trnG-nad3 in Lipotactes tripyrga (Lipotactinae), were identified from 59 ensiferan mitogenomes...
March 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28179182/chloroplast-and-its-phylogenies-to-understand-the-evolutionary-history-of-southern-south-american-azorella-laretia-and-mulinum-azorelloideae-apiaceae
#7
Martina Fernández, Cecilia Ezcurra, Carolina I Calviño
Azorella, Laretia and Mulinum are taxonomically complex, and good candidates to study evolutionary radiations in the Andes and the importance of hybridizations. Previous phylogenetic studies of subfamily Azorelloideae agree that Azorella and Mulinum as currently conceived are not monophyletic, and hence a revision of their circumscription is necessary. However, these phylogenies were based only on chloroplast DNA sequence data. Here, phylogenetic relationships within Azorelloideae were inferred using sequence data from five chloroplast DNA (rps16 intron, trnQ-rps16, rps16-trnK(UUU) 5' -exon, trnG(GCC)-trnS(GCU) and rpL32-trnL(UAG)), and from nuclear rDNA ITS regions to assess the monophyly of Azorella and Mulinum and discuss generic re-circumscriptions, determine hybridization and radiation events, identify and characterize important lineages, and propose hypotheses on evolution of key morphological characters...
March 2017: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/28122062/hedysarum-l-fabaceae-hedysareae-is-not-monophyletic-evidence-from-phylogenetic-analyses-based-on-five-nuclear-and-five-plastid-sequences
#8
Pei-Liang Liu, Jun Wen, Lei Duan, Emine Arslan, Kuddisi Ertuğrul, Zhao-Yang Chang
The legume family (Fabaceae) exhibits a high level of species diversity and evolutionary success worldwide. Previous phylogenetic studies of the genus Hedysarum L. (Fabaceae: Hedysareae) showed that the nuclear and the plastid topologies might be incongruent, and the systematic position of the Hedysarum sect. Stracheya clade was uncertain. In this study, phylogenetic relationships of Hedysarum were investigated based on the nuclear ITS, ETS, PGDH, SQD1, TRPT and the plastid psbA-trnH, trnC-petN, trnL-trnF, trnS-trnG, petN-psbM sequences...
2017: PloS One
https://www.readbyqxmd.com/read/28111318/in-silico-predicted-reproductive-endocrine-transcriptional-regulatory-networks-during-zebrafish-danio-rerio-development
#9
D Hala
The interconnected topology of transcriptional regulatory networks (TRNs) readily lends to mathematical (or in silico) representation and analysis as a stoichiometric matrix. Such a matrix can be 'solved' using the mathematical method of extreme pathway (ExPa) analysis, which identifies uniquely activated genes subject to transcription factor (TF) availability. In this manuscript, in silico multi-tissue TRN models of brain, liver and gonad were used to study reproductive endocrine developmental programming in zebrafish (Danio rerio) from 0...
January 19, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28072813/a-case-report-on-red-ear-syndrome-with-tinnitus-successfully-treated-with-transcranial-random-noise-stimulation
#10
Peter M Kreuzer, Veronika Vielsmeier, Timm B Poeppl, Berthold Langguth
BACKGROUND: The red ear syndrome represents a rare symptom complex consisting of auricular erythema associated with painful and burning sensations. It has been described in combination with tinnitus rarely. It has been hypothesized to be etiologically related to altered trigeminal afferent input, temporomandibular disorders, and thalamic dysfunction. OBJECTIVES: The initial objective of applying transcranial random noise stimulation (tRNS) in a case of red ear syndrome in combination with tinnitus was the alleviation of the phantom sounds...
January 2017: Pain Physician
https://www.readbyqxmd.com/read/27965082/analysis-of-whole-chloroplast-genomes-from-the-genera-of-the-clauseneae-the-curry-tribe-rutaceae-citrus-family
#11
Vikram S Shivakumar, Marc S Appelhans, Gabriel Johnson, Monica Carlsen, Elizabeth A Zimmer
The Clauseneae (Aurantioideae, Rutaceae) is a tribe in the Citrus family that, although economically important as it contains the culinary and medicinally-useful curry tree (Bergera koenigii), has been relatively understudied. Due to the recent significant taxonomic changes made to this tribe, a closer inspection of the genetic relationships among its genera has been warranted. Whole genome skimming was used to generate chloroplast genomes from six species, representing each of the four genera (Bergera, Clausena, Glycosmis, Micromelum) in the Clauseneae tribe plus one closely related outgroup (Merrillia), using the published plastome sequence of Citrus sinensis as a reference...
December 11, 2016: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/27934947/opposite-effects-of-high-and-low-frequency-transcranial-random-noise-stimulation-probed-with-visual-motion-adaptation
#12
Gianluca Campana, Rebecca Camilleri, Beatrice Moret, Filippo Ghin, Andrea Pavan
Transcranial random noise stimulation (tRNS) is a recent neuro-modulation technique whose effects at both behavioural and neural level are still debated. Here we employed the well-known phenomenon of motion after-effect (MAE) in order to investigate the effects of high- vs. low-frequency tRNS on motion adaptation and recovery. Participants were asked to estimate the MAE duration following prolonged adaptation (20 s) to a complex moving pattern, while being stimulated with either sham or tRNS across different blocks...
December 9, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27912894/self-generation-and-positivity-effects-following-transcranial-random-noise-stimulation-in-medial-prefrontal-cortex-a-reality-monitoring-task-in-older-adults
#13
Nicola Mammarella, Alberto Di Domenico, Rocco Palumbo, Beth Fairfield
Activation of medial Prefrontal Cortex (mPFC) has been typically found during reality monitoring tasks (i.e., distinguishing between internal self-generated vs external information). No study, however, has yet investigated whether transcranial Random Noise Stimulation (tRNS) over the mPFC leads to a reduction in reality-monitoring misattributions in aging. In particular, stimulating mPFC should increase the number of cognitive operations engaged while encoding and this distinctive information may help older adults to discriminate between internal and external sources better...
November 15, 2016: Cortex; a Journal Devoted to the Study of the Nervous System and Behavior
https://www.readbyqxmd.com/read/27899929/efficient-identification-of-the-forest-tree-species-in-aceraceae-using-dna-barcodes
#14
Yu-Wei Han, Dong Duan, Xiong-Feng Ma, Yun Jia, Zhan-Lin Liu, Gui-Fang Zhao, Zhong-Hu Li
Aceraceae is a large forest tree family that comprises many economically and ecologically important species. However, because interspecific and/or intraspecific morphological variations result from frequent interspecific hybridization and introgression, it is challenging for non-taxonomists to accurately recognize and identify the tree species in Aceraceae based on a traditional approach. DNA barcoding is a powerful tool that has been proposed to accurately distinguish between species. In this study, we assessed the effectiveness of three core standard markers (matK, rbcL and ITS) plus the chloroplast locus trnS-trnG as Aceraceae barcodes...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27827992/the-complete-mitochondrial-genome-of-aleurocanthus-camelliae-insights-into-gene-arrangement-and-genome-organization-within-the-family-aleyrodidae
#15
Shi-Chun Chen, Xiao-Qing Wang, Pin-Wu Li, Xiang Hu, Jin-Jun Wang, Ping Peng
There are numerous gene rearrangements and transfer RNA gene absences existing in mitochondrial (mt) genomes of Aleyrodidae species. To understand how mt genomes evolved in the family Aleyrodidae, we have sequenced the complete mt genome of Aleurocanthus camelliae and comparatively analyzed all reported whitefly mt genomes. The mt genome of A. camelliae is 15,188 bp long, and consists of 13 protein-coding genes, two rRNA genes, 21 tRNA genes and a putative control region (GenBank: KU761949). The tRNA gene, trnI, has not been observed in this genome...
November 7, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27770241/the-complete-mitochondrial-genome-of-trabala-vishnou-guttata-lepidoptera-lasiocampidae-and-the-related-phylogenetic-analyses
#16
Liuyu Wu, Xiao Xiong, Xuming Wang, Tianrong Xin, Jing Wang, Zhiwen Zou, Bin Xia
The bluish yellow lappet moth, Trabala vishnou guttata is an extraordinarily important pest in China. The complete mitochondrial genome is sequenced and determined firstly, which is based on traditional PCR amplification and primer walking methods with a length of 15,281 bp, including 13 protein-coding (PCG) genes, 22 transfer RNA (rRNA) genes, two ribosomal RNA (tRNA) genes, and an A + T-rich region. The gene order and orientation of the T. vishnou guttata mitogenome were identical to the other sequenced Lasiocampidae species...
December 2016: Genetica
https://www.readbyqxmd.com/read/27761741/the-added-value-of-auditory-cortex-transcranial-random-noise-stimulation-trns-after-bifrontal-transcranial-direct-current-stimulation-tdcs-for-tinnitus
#17
Wing Ting To, Jan Ost, John Hart, Dirk De Ridder, Sven Vanneste
Tinnitus is the perception of a sound in the absence of a corresponding external sound source. Research has suggested that functional abnormalities in tinnitus patients involve auditory as well as non-auditory brain areas. Transcranial electrical stimulation (tES), such as transcranial direct current stimulation (tDCS) to the dorsolateral prefrontal cortex and transcranial random noise stimulation (tRNS) to the auditory cortex, has demonstrated modulation of brain activity to transiently suppress tinnitus symptoms...
October 19, 2016: Journal of Neural Transmission
https://www.readbyqxmd.com/read/27603700/applying-dna-barcodes-to-identify-closely-related-species-of-ferns-a-case-study-of-the-chinese-adiantum-pteridaceae
#18
Fan-Hong Wang, Jin-Mei Lu, Jun Wen, Atsushi Ebihara, De-Zhu Li
DNA barcoding is a fast-developing technique to identify species by using short and standard DNA sequences. Universal selection of DNA barcodes in ferns remains unresolved. In this study, five plastid regions (rbcL, matK, trnH-psbA, trnL-F and rps4-trnS) and eight nuclear regions (ITS, pgiC, gapC, LEAFY, ITS2, IBR3_2, DET1, and SQD1_1) were screened and evaluated in the fern genus Adiantum from China and neighboring areas. Due to low primer universality (matK) and/or the existence of multiple copies (ITS), the commonly used barcodes matK and ITS were not appropriate for Adiantum...
2016: PloS One
https://www.readbyqxmd.com/read/27571244/relationship-between-internal-load-indicators-and-changes-on-intermittent-performance-after-the-preseason-in-professional-soccer-players
#19
Miguel A Campos-Vazquez, Francisco J Toscano-Bendala, José Carlos Mora-Ferrera, Luis Suarez-Arrones
The aim of this study was to examine the effects of accumulated internal training load during the preseason (4 weeks) on changes in the intermittent performance, in a professional soccer team. Twelve professionals soccer players (Mean±SD age 27.7±4.3 years; height: 177.1±6.2 cm; body mass: 73.1±5.2 kg; % body fat (Faulkner): 10.2±1.2) belonging to a Spanish second division team (2013-2014) participated in this study. The 30-15 intermittent fitness test was performed before and after the preseason, and the speed for the last period completed by each player was recorded (VIFT)...
August 22, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27496671/imaging-transcranial-direct-current-stimulation-tdcs-of-the-prefrontal-cortex-correlation-or-causality-in-stimulation-mediated-effects
#20
Jana Wörsching, Frank Padberg, Birgit Ertl-Wagner, Ulrike Kumpf, Beatrice Kirsch, Daniel Keeser
Transcranial current stimulation approaches include neurophysiologically distinct non-invasive brain stimulation techniques widely applied in basic, translational and clinical research: transcranial direct current stimulation (tDCS), oscillating transcranial direct current stimulation (otDCS), transcranial alternating current stimulation (tACS) and transcranial random noise stimulation (tRNS). Prefrontal tDCS seems to be an especially promising tool for clinical practice. In order to effectively modulate relevant neural circuits, systematic research on prefrontal tDCS is needed that uses neuroimaging and neurophysiology measures to specifically target and adjust this method to physiological requirements...
October 2016: Neuroscience and Biobehavioral Reviews
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