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https://www.readbyqxmd.com/read/29409808/long-non-coding-rna-linc00460-promotes-epithelial-mesenchymal-transition-and-cell-migration-in-lung-cancer-cells
#1
Ke Li, Dan Sun, Qiheng Gou, Xixian Ke, Yanqiu Gong, Yuanli Zuo, Jian-Kang Zhou, Chenglin Guo, Zhichu Xia, Lunxu Liu, Qintong Li, Lunzhi Dai, Yong Peng
Long non-coding RNAs (lncRNAs) function as critical regulators to participate in tumor progression and metastasis. However, their roles in non-small cell lung cancer (NSCLC) are poorly understood. In this study, we found that the expression of the lncRNA linc00460 is significantly upregulated in NSCLC tumors and associated with poor prognosis for NSCLC patients, implying that linc00460 is important for lung cancer development. The accurate transcription initiation and termination sites of linc00460 were then identified by rapid-amplification of cDNA ends (RACE) technologies, and the sequencing data demonstrated that linc00460, predominantly located in the cytoplasm of lung cancer cells, is a novel transcript variant...
January 31, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29396485/hnrnp-a1-a2-and-sam68-collaborate-with-srsf10-to-control-the-alternative-splicing-response-to-oxaliplatin-mediated-dna-damage
#2
Alexandre Cloutier, Lulzim Shkreta, Johanne Toutant, Mathieu Durand, Philippe Thibault, Benoit Chabot
Little is known about how RNA binding proteins cooperate to control splicing, and how stress pathways reconfigure these assemblies to alter splice site selection. We have shown previously that SRSF10 plays an important role in the Bcl-x splicing response to DNA damage elicited by oxaliplatin in 293 cells. Here, RNA affinity assays using a portion of the Bcl-x transcript required for this response led to the recovery of the SRSF10-interacting protein 14-3-3ε and the Sam68-interacting protein hnRNP A1. Although SRSF10, 14-3-3ε, hnRNP A1/A2 and Sam68 do not make major contributions to the regulation of Bcl-x splicing under normal growth conditions, upon DNA damage they become important to activate the 5' splice site of pro-apoptotic Bcl-xS...
February 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29396052/tgf-beta-signaling-in-cancer-post-transcriptional-regulation-of-emt-via-hnrnp-e1
#3
Breege V Howley, Philip H Howe
The TGFβ signaling pathway is a critical regulator of cancer progression in part through induction of the epithelial to mesenchymal transition (EMT). This process is aberrantly activated in cancer cells, facilitating invasion of the basement membrane, survival in the circulatory system, and dissemination to distant organs. The mechanisms through which epithelial cells transition to a mesenchymal state involve coordinated transcriptional and post-transcriptional control of gene expression. One such mechanism of control is through the RNA binding protein hnRNP E1, which regulates splicing and translation of a cohort of EMT and stemness-associated transcripts...
January 27, 2018: Cytokine
https://www.readbyqxmd.com/read/29379020/molecular-basis-for-the-specific-and-multivariant-recognitions-of-rna-substrates-by-human-hnrnp-a2-b1
#4
Baixing Wu, Shichen Su, Deepak P Patil, Hehua Liu, Jianhua Gan, Samie R Jaffrey, Jinbiao Ma
Human hnRNP A2/B1 is an RNA-binding protein that plays important roles in many biological processes, including maturation, transport, and metabolism of mRNA, and gene regulation of long noncoding RNAs. hnRNP A2/B1 was reported to control the microRNAs sorting to exosomes and promote primary microRNA processing as a potential m6A "reader." hnRNP A2/B1 contains two RNA recognition motifs that provide sequence-specific recognition of RNA substrates. Here, we determine crystal structures of tandem RRM domains of hnRNP A2/B1 in complex with various RNA substrates, elucidating specific recognitions of AGG and UAG motifs by RRM1 and RRM2 domains, respectively...
January 29, 2018: Nature Communications
https://www.readbyqxmd.com/read/29366917/functional-characterization-of-naturally-occurring-genetic-variations-of-the-human-guanine-rich-rna-sequence-binding-factor-1-grsf1
#5
Sajad Sofi, Julia C Fitzgerald, Désirée Jähn, Bernhard Dumoulin, Sabine Stehling, Hartmut Kuhn, Christoph Ufer
The guanine-rich RNA sequence binding factor 1 (GRSF1) constitutes an ubiquitously occurring RNA-binding protein (RBP), which belongs to the family of heterogeneous nuclear ribonucleoprotein F/H (hnRNP F/H). It has been implicated in nuclear, cytosolic and mitochondrial RNA metabolism. Although the crystal structures of GRSF1 orthologs have not been solved, amino acid alignments with similar RNA-binding proteins suggested the existence of three RNA-binding domains designated quasi-RNA recognition motifs (qRRMs)...
January 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29358076/mechanistic-view-of-hnrnpa2-low-complexity-domain-structure-interactions-and-phase-separation-altered-by-mutation-and-arginine-methylation
#6
Veronica H Ryan, Gregory L Dignon, Gül H Zerze, Charlene V Chabata, Rute Silva, Alexander E Conicella, Joshua Amaya, Kathleen A Burke, Jeetain Mittal, Nicolas L Fawzi
hnRNPA2, a component of RNA-processing membraneless organelles, forms inclusions when mutated in a syndrome characterized by the degeneration of neurons (bearing features of amyotrophic lateral sclerosis [ALS] and frontotemporal dementia), muscle, and bone. Here we provide a unified structural view of hnRNPA2 self-assembly, aggregation, and interaction and the distinct effects of small chemical changes-disease mutations and arginine methylation-on these assemblies. The hnRNPA2 low-complexity (LC) domain is compact and intrinsically disordered as a monomer, retaining predominant disorder in a liquid-liquid phase-separated form...
January 17, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29357978/reccurrent-f8-intronic-deletion-found-in-mild-hemophilia-a-causes-alu-exonization
#7
Yohann Jourdy, Alexandre Janin, Mathilde Fretigny, Anne Lienhart, Claude Négrier, Dominique Bozon, Christine Vinciguerra
Incorporation of distant intronic sequences in mature mRNA is an underappreciated cause of genetic disease. Several disease-causing pseudoexons have been found to contain repetitive elements such as Alu elements. This study describes an original pathological mechanism by which a small intronic deletion leads to Alu exonization. We identified an intronic deletion, c.2113+461_2113+473del, in the F8 intron 13, in two individuals with mild hemophilia A. In vivo and in vitro transcript analysis found an aberrant transcript, with an insertion of a 122-bp intronic fragment (c...
January 8, 2018: American Journal of Human Genetics
https://www.readbyqxmd.com/read/29344170/phosphorylation-of-ser6-in-hnrnpa1-by-s6k2-regulates-glucose-metabolism-and-cell-growth-in-colorectal-cancer
#8
Yan Sun, Man Luo, Guilin Chang, Weiying Ren, Kefen Wu, Xi Li, Jiping Shen, Xiaoping Zhao, Yu Hu
Abnormal glucose metabolism is critical in colorectal cancer (CRC) development. Expression of the pyruvate kinase (PK) M2 isoform, rather than the PKM1 isoform, serves important functions in reprogramming the glucose metabolism of cancer cells. Preferential expression of PKM2 is primarily driven by alternative splicing, which is coordinated by a group of splicing factors including heterogeneous nuclear ribonucleoprotein (hnRNP)A1, hnRNPA2 and RNA binding motif containing. However, the underlying molecular mechanisms associated with cancer cell expression of PKM2, instead of PKM1, remain unknown...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29331391/microrna-mediated-regulation-of-splicing-factors-srsf1-srsf2-and-hnrnp-a1-in-context-of-their-alternatively-spliced-3-utrs
#9
Elżbieta Sokół, Hanna Kędzierska, Alicja Czubaty, Beata Rybicka, Katarzyna Rodzik, Zbigniew Tański, Joanna Bogusławska, Agnieszka Piekiełko-Witkowska
SRSF1, SRSF2 and hnRNP A1 are splicing factors that regulate the expression of oncogenes and tumor suppressors. SRSF1 and SRSF2 contribute to the carcinogenesis in the kidney. Despite their importance, the mechanisms regulating their expression in cancer are not entirely understood. Here, we investigated the microRNA-mediated regulation of SRSF1, SRSF2 and hnRNP A1 in renal cancer. The expression of microRNAs predicted to target SRSF1, SRSF2 and hnRNP A1 was disturbed in renal tumors compared with controls...
January 10, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29328485/knockdown-of-hnrnp-a2-b1-inhibits-cell-proliferation-invasion-and-cell-cycle-triggering-apoptosis-in-cervical-cancer-via-pi3k-akt-signaling-pathway
#10
Xiang Shi, Li Ran, Yao Liu, Shu-Huai Zhong, Ping-Ping Zhou, Ming-Xin Liao, Wen Fang
Cervical cancer is currently one of the major threats to women's health. The overexpression of heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) as the biomarker has been investigated in various cancers. In our previous study, we found that lobaplatin induced apoptosis and cell cycle arrest via downregulation of proteins including hnRNP A2/B1 in cervical cancer cells. However, the underlying relationship between hnRNP A2/B1 and cervical cancer remained largely unknown. hnRNP A2/B1 knock‑down in HeLa and CaSki cells was performed by shRNA transfection...
January 5, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29322631/cold-inducible-rna-binding-protein-in-cancer-and-inflammation
#11
REVIEW
Daniel A Lujan, Joey L Ochoa, Rebecca S Hartley
RNA binding proteins (RBPs) play key roles in RNA dynamics, including subcellular localization, translational efficiency and metabolism. Cold-inducible RNA binding protein (CIRP) is a stress-induced protein that was initially described as a DNA damage-induced transcript (A18 hnRNP), as well as a cold-shock domain containing cold-stress response protein (CIRBP) that alters the translational efficiency of its target messenger RNAs (mRNAs). This review summarizes recent work on the roles of CIRP in the context of inflammation and cancer...
January 11, 2018: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29321325/the-identification-and-characterization-of-sindbis-virus-rna-host-protein-interactions
#12
Autumn T LaPointe, Natasha N Gebhart, Megan E Meller, Richard W Hardy, Kevin J Sokoloski
Arthropod-borne viruses, such as the members of genus Alphavirus, are a significant concern to global public health. As obligate intracellular pathogens, RNA viruses must interact with the host cell machinery to establish, and complete, their viral lifecycles. Despite considerable efforts to define the host/pathogen interactions essential for alphaviral replication, an unbiased and inclusive assessment of alphaviral RNA:protein interactions has not been undertaken. Moreover, the biological and molecular importance of these interactions, in the full context of their molecular function as RNA-binding proteins, has not been fully realized...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29302256/heterogeneous-nuclear-ribonucleoprotein-a2b1-exerts-a-regulatory-role-in-lipopolysaccharide-stimulated-38b9-b-cell-activation
#13
Jisang Park, Chung-Hyeon Choe, Ju Kim, Jing Shian Yang, Jin Hyun Kim, Hyonseok Jang, Yong-Suk Jang
Major histocompatibility complex (MHC) class II molecules, which are recognized for their primary function of presenting an antigen to the T cell receptor, are involved in various signaling pathways in B cell activation. We identified heterogeneous nuclear ribonucleoprotein (hnRNP) A2B1 as an MHC class II molecule-associated protein involved in MHC class II-mediated signal transduction in lipopolysaccharide (LPS)-stimulated 38B9 B cells. Although the function of hnRNP A2B1 in the nucleus is primarily known, the level of hnRNP A2B1 in the cytoplasm was increased in LPS-stimulated 38B9 cells, while it was not detected in the cytoplasm of non-treated 38B9 cells...
December 2017: Immune Network
https://www.readbyqxmd.com/read/29296177/isg15-silencing-increases-cisplatin-resistance-via-activating-p53-mediated-cell-dna-repair
#14
Yi Huo, Zhaoyun Zong, Qingtao Wang, Zhenyu Zhang, Haiteng Deng
Tumor cells frequently evolved resistance to cisplatin that greatly compromises the efficacy of chemotherapy. Identification of the mechanisms underlying drug resistance is important for developing new therapeutic approaches. ISG15 is found to be elevated in many human carcinomas and cancer cell lines. Here, we identified that the expressions of ISG15 and ISG15-conjugating system were downregulated in drug resistant A549/DDP cells compared to drug sensitive A549 cells. Silencing of ISG15 robustly elevated the resistance to cisplatin, suggesting ISG15 plays an important role in cisplatin resistance...
December 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/29229447/hnrnp-a1-promotes-keratinocyte-cell-survival-post-uvb-radiation-through-pi3k-akt-mtor-pathway
#15
Jianguo Feng, Yi Liao, Xichao Xu, Qian Yi, Ling He, Liling Tang
hnRNP A1 acts as a critical splicing factor in regulating many alternative splicing events in various physiological and pathophysiological progressions. hnRNP A1 is capable of regulating UVB-induced hdm2 gene alternative splicing according to our previous study. However, the biological function and underlying molecular mechanism of hnRNP A1 in cell survival and cell cycle in response to UVB irradiation are still unclear. In this study, silencing hnRNP A1 expression by siRNA transfection led to decreased cell survival after UVB treatment, while promoting hnRNP A1 by lentiviruse vector resulted in increased cell survival...
December 8, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29228625/long-noncoding-rna-sfta1p-promoted-apoptosis-and-increased-cisplatin-chemosensitivity-via-regulating-the-hnrnp-u-gadd45a-axis-in-lung-squamous-cell-carcinoma
#16
Ling Li, Ji-Ye Yin, Fa-Zhong He, Ma-Sha Huang, Tao Zhu, Yuan-Feng Gao, Yi-Xin Chen, Dong-Bo Zhou, Xiang Chen, Lun-Quan Sun, Wei Zhang, Hong-Hao Zhou, Zhao-Qian Liu
Chemotherapeutic insensitivity remains one of the major obstacles in clinical treatment of lung squamous cell carcinoma (LSCC). Recently, increasing evidence has suggested that long non-coding RNAs (lncRNAs) promote tumorigenesis in many cancer types. However, the potential biological roles and regulatory mechanisms of lncRNAs in response to cisplatin treatment are poorly understood. Here, we found that lncRNA SFTA1P (surfactant associated 1, pseudogene), highly expressed in lung, was down-regulated in LSCC tissues and could be induced upon cisplatin treatment in LSCC cells...
November 14, 2017: Oncotarget
https://www.readbyqxmd.com/read/29217678/hrpu-2-a-homologue-of-mammalian-hnrnp-u-regulates-synaptic-transmission-by-controlling-the-expression-of-slo-2-potassium-channel-in-c-elegans
#17
Ping Liu, Sijie Jason Wang, Zhao-Wen Wang, Bojun Chen
Slo2 channels are large-conductance potassium channels abundantly expressed in the nervous system. However, it is unclear how their expression level in neurons is regulated. Here we report that HRPU-2, an RNA-binding protein homologous to mammalian hnRNP U, plays an important role in regulating the expression of SLO-2 (a homologue of mammalian Slo2) in C. elegans Loss-of-function (lf) mutants of hrpu-2 were isolated in a genetic screen for suppressors of a sluggish phenotype caused by a hyperactive SLO-2. In hrpu-2(lf) mutants, SLO-2-mediated delayed outward currents in neurons are greatly decreased, and neuromuscular synaptic transmission is enhanced...
December 7, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29213352/echinacoside-alleviates-uvb-irradiation-mediated-skin-damage-via-inhibition-of-oxidative-stress-dna-damage-and-apoptosis
#18
Di Zhang, Chengtao Lu, Zhe Yu, Xiayin Wang, Li Yan, Juanli Zhang, Hua Li, Jianbo Wang, Aidong Wen
Ultraviolet B (UVB) irradiation has been known to cause skin damage, which is associated with oxidative stress, DNA damage, and apoptosis. Echinacoside is a phenylethanoid glycoside isolated from Herba Cistanches, which exhibits strong antioxidant activity. In this study, we evaluate the photoprotective effect of echinacoside on UVB-induced skin damage and explore the potential molecular mechanism. BALB/c mice and HaCaT cells were treated with echinacoside before UVB exposure. Histopathological examination was used to evaluate the skin damage...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29191962/nontypeable-haemophilus-influenzae-lysates-increase-heterogeneous-nuclear-ribonucleoprotein-secretion-and-exosome-release-in-human-middle-ear-epithelial-cells
#19
Stéphanie Val, Anna Krueger, Marian Poley, Ariella Cohen, Kristy Brown, Aswini Panigrahi, Diego Preciado
Nontypeable Haemophilus influenzae (NTHi), one of the most common acute otitis media (OM) pathogens, is postulated to promote middle-ear epithelial remodeling in the progression of OM from acute to chronic. The goal of this study was to examine early quantitative proteomic secretome effects of NTHi lysate exposure in a human middle-ear epithelial cell (HMEEC) line. NTHi lysates were used to stimulate HMEEC, and conditional quantitative stable isotope labeling with amino acids in cell culture of cell secretions was performed...
November 30, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29187863/smooth-an-hnrnp-l-homolog-might-decrease-mitochondrial-metabolism-by-post-transcriptional-regulation-of-isocitrate-dehydrogenase-idh-and-other-metabolic-genes-in-the-sub-acute-phase-of-traumatic-brain-injury
#20
Arko Sen, Katherine Gurdziel, Jenney Liu, Wen Qu, Oluwademi O Nuga, Rayanne B Burl, Maik Hüttemann, Roger Pique-Regi, Douglas M Ruden
Traumatic brain injury (TBI) can cause persistent pathological alteration of neurons. This may lead to cognitive dysfunction, depression and increased susceptibility to life threatening diseases, such as epilepsy and Alzheimer's disease. To investigate the underlying genetic and molecular basis of TBI, we subjected w1118Drosophila melanogaster to mild closed head trauma and found that mitochondrial activity is reduced in the brains of these flies 24 h after inflicting trauma. To determine the transcriptomic changes after mild TBI, we collected fly heads 24 h after inflicting trauma, and performed RNA-seq analyses...
2017: Frontiers in Genetics
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