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https://www.readbyqxmd.com/read/28447190/regulation-of-neat1-mir-214-3p-on-the-growth-migration-and-invasion-of-endometrial-carcinoma-cells
#1
Jian Wang, Xiangzhai Zhao, Zhaojun Guo, Xiaolin Ma, Yueqing Song, Ying Guo
OBJECTIVE: To investigate the function and mechanism of lnc NEAT1 in regulating the growth, migration and invasion of endometrial carcinoma (EC) cells. MATERIALS AND METHODS: NEAT1 and miR-214-3p levels were measured by qRT-PCR. The protein levels of HMGA1, β-catenin, c-myc and MMP9 were evaluated by Western blot. The effects of NEAT1, HMGA1, miR-214-3p on the viability, migration and invasion of HEC-1A cells were accessed by WST-1 assay and transwell migration/invasion assay...
April 26, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28423658/correlation-between-polymorphisms-in-microrna-regulated-genes-and-cervical-cancer-susceptibility-in-a-xinjiang-uygur-population
#2
Jing Fang, Ying Li, Jiayi Zhang, Mengdan Yan, Jingjie Li, Shan Bao, Tianbo Jin
We explored the correlation between single nucleotide polymorphisms (SNPs) and susceptibility to cervical cancer (CC) in a Xinjiang Uygur population. Ten SNPs in eight miRNA-regulated genes were selected for analysis. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated using unconditional logistic regression analysis. Multivariate logistic regression analysis was used to detect correlations between SNPs and CC. We found that minor allele "C" of rs512715 in NEAT1 was associated with an increased risk of CC in the allele, codominant, dominant, overdominant and log-additive models...
March 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/28418939/exploring-the-molecular-pathogenesis-and-biomarkers-of-high-risk-oral-premalignant-lesions-on-the-basis-of-long-noncoding-rna-expression-profiling-by-serial-analysis-of-gene-expression
#3
Hongcheng Jia, Xuan Wang, Zheng Sun
Oral premalignant lesions (OPLs) have malignant transformation potential, with no reliable markers available. This study aimed to assess molecular events to identify biomarkers that can reflect high-risk lesions as predictive factors to tailor clinical decision for patients on the basis of long noncoding RNAs (lncRNA) expression profiling by serial analysis of gene expression. The GSE31021 and GSE8127 datasets were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) and lncRNAs were identified using the LIMMA package in R language...
April 24, 2017: European Journal of Cancer Prevention
https://www.readbyqxmd.com/read/28404604/unusual-semi-extractability-as-a-hallmark-of-nuclear-body-associated-architectural-noncoding-rnas
#4
Takeshi Chujo, Tomohiro Yamazaki, Tetsuya Kawaguchi, Satoshi Kurosaka, Toru Takumi, Shinichi Nakagawa, Tetsuro Hirose
NEAT1_2 long noncoding RNA (lncRNA) is the molecular scaffold of paraspeckle nuclear bodies. Here, we report an improved RNA extraction method: extensive needle shearing or heating of cell lysate in RNA extraction reagent improved NEAT1_2 extraction by 20-fold (a property we term "semi-extractability"), whereas using a conventional method NEAT1_2 was trapped in the protein phase. The improved extraction method enabled us to estimate that approximately 50 NEAT1_2 molecules are present in a single paraspeckle...
April 12, 2017: EMBO Journal
https://www.readbyqxmd.com/read/28401449/silence-of-long-noncoding-rna-neat1-inhibits-malignant-biological-behaviors-and-chemotherapy-resistance-in-gastric-cancer
#5
Jiale Zhang, Bochao Zhao, Xiuxiu Chen, Zhenning Wang, Huimian Xu, Baojun Huang
Gastric cancer (GC) is the most common solid tumor in digestive system. Nuclear-enriched abundant transcript 1 (NEAT1) gene is a lncRNA, and reveal potential oncogene role in several malignant tumors. The aim of this study is to investigate the expression and clinical significance of Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) gene and its influence to malignant biologic behaviors and chemotherapy resistance to adriamycin in GC. This study found NEAT1 was up-regulated in GC tissues and cells, especially in in GC adriamycin-resistant cells...
April 11, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28379604/neat1-mir-181c-regulates-osteopontin-opn-mediated-synoviocyte-proliferation-in-osteoarthritis
#6
Qiyuan Wang, Wanchun Wang, Fan Zhang, Youwen Deng, Zeling Long
Osteoarthritis (OA) is characterized by progressive destruction of articular cartilage, resulting in significant disability. Inflammatory cytokines commonly initiate the extreme changes in the synovium and cartilage microenvironment of the OA patients, subsequently resulting in cell dysfunctions, especially synoviocyte dysfunction. We revealed that the expression of osteopontin (OPN), which has been reported to regulate expression of various inflammatory factors associating with the pathogenesis of OA including matrix metalloprotease 13 (MMP13), interlukine-6 and 8 (IL-6 and IL-8), is significantly upregulated in OA tissues...
April 5, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28358873/whole-genome-analysis-of-papillary-kidney-cancer-finds-significant-noncoding-alterations
#7
Shantao Li, Brian M Shuch, Mark B Gerstein
To date, studies on papillary renal-cell carcinoma (pRCC) have largely focused on coding alterations in traditional drivers, particularly the tyrosine-kinase, Met. However, for a significant fraction of tumors, researchers have been unable to determine a clear molecular etiology. To address this, we perform the first whole-genome analysis of pRCC. Elaborating on previous results on MET, we find a germline SNP (rs11762213) in this gene predicting prognosis. Surprisingly, we detect no enrichment for small structural variants disrupting MET...
March 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28349920/profiling-the-mapk-erk-dependent-and-independent-activity-regulated-transcriptional-programs-in-the-murine-hippocampus-in-vivo
#8
Nils Blüthgen, Mirjam van Bentum, Barbara Merz, Dietmar Kuhl, Guido Hermey
Activity-dependent alteration of the transcriptional program is central for shaping neuronal connectivity. Constitutively expressed transcription factors orchestrate the initial response to neuronal stimulation and serve as substrates for second messenger-regulated kinase signalling cascades. The mitogen-activated protein kinase ERK conveys signalling from the synapse to the nucleus but its genetic signature following neuronal activity has not been revealed. The goal of the present study was to identify ERK dependent and independent activity regulated transcriptional programs in the murine hippocampus...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28338194/lncrna-neat1-is-closely-related-with-progression-of-breast-cancer-via-promoting-proliferation-and-emt
#9
M Zhang, W-B Wu, Z-W Wang, X-H Wang
OBJECTIVE: Breast cancer (BC) is one of the most common cancers in females. Abnormal proliferation and metastasis are key reasons that cause mortality in BC patients. More and more evidence showed that lncRNA played an important role in the BC development, but the mechanism was not well established. PATIENTS AND METHODS: The expression of lncRNA NEAT1 was detected in 40 BC patients by qRT-PCR technology. MTT and Wound Healing assays were applied to detect the effect of lncRNA NEAT1 in proliferation and metastasis in BC...
March 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28325845/functional-dissection-of-neat1-using-genome-editing-reveals-substantial-localisation-of-the-neat1_1-isoform-outside-paraspeckles
#10
Ruohan Li, Alan R Harvey, Stuart I Hodgetts, Archa H Fox
Large numbers of long non-coding RNAs have been discovered in recent years, but only a few have been characterised. NEAT1 (nuclear paraspeckle assembly transcript 1) is a mammalian long non-coding RNA that is important for the reproductive physiology of mice, cancer development and the formation of subnuclear bodies termed paraspeckles. The two major isoforms of NEAT1 (3.7kb NEAT1_1 and 23kb NEAT1_2 in human) are generated from a common promoter and are produced through the use of alternative transcription termination sites...
March 21, 2017: RNA
https://www.readbyqxmd.com/read/28295289/long-non-coding-rna-neat1-is-a-transcriptional-target-of-p53-and-modulates-p53-induced-transactivation-and-tumor-suppressor-function
#11
Masashi Idogawa, Tomoko Ohashi, Yasushi Sasaki, Hiroshi Nakase, Takashi Tokino
p53 is one of the most important tumor suppressor genes, and the direct transcriptional targets of p53 must be explored to elucidate its functional mechanisms. Thus far, the p53 targets that have been primarily studied are protein-coding genes. Our previous study revealed that several long non-coding RNAs (lncRNAs) are direct transcriptional targets of p53, and knockdown of specific lncRNAs modulates p53-induced apoptosis. In this study, analysis of next-generation chromatin immunoprecipitation-sequencing (ChIP-seq) data for p53 revealed that the lncRNA NEAT1 is a direct transcriptional target of p53...
March 14, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28288210/depletion-of-neat1-lncrna-attenuates-nucleolar-stress-by-releasing-sequestered-p54nrb-and-psf-to-facilitate-c-myc-translation
#12
Wen Shen, Xue-Hai Liang, Hong Sun, Cheryl L De Hoyos, Stanley T Crooke
Altered expression of NEAT1, the architectural long non-coding RNA (lncRNA) of nuclear paraspeckles, has been reported during tumorigenesis, as well as under various cellular stress conditions. Here we report that the depletion of NEAT1 lncRNA alleviates nucleolar stress during RNAP I inhibition through releasing sequestered P54nrb and PSF to facilitate the IRES-dependent translation of c-Myc. RNAP I inhibitor CX5461 disrupts the SL1-rDNA interaction and induces nucleolar disruption, demonstrated by the accumulation of fibrillarin-containing nucleoplasmic foci and nucleolar clearance of ribosomal proteins in HeLa cells...
2017: PloS One
https://www.readbyqxmd.com/read/28269753/lncrna-neat1-is-a-prognosis-biomarker-and-regulates-cancer-progression-via-epithelial-mesenchymal-transition-in-clear-cell-renal-cell-carcinoma
#13
Li Ning, Zhiguo Li, Dianjun Wei, Haiyan Chen, Chao Yang
BACKGROUND: The long non-coding RNAs (lncRNAs) are emerging as important regulators in cancer progression. Clear cell renal cell carcinoma (ccRCC) is one of the most common urological cancers with poor prognosis. In this study, we examined the functional role of the lncRNA, nuclear enriched abundant transcript 1 (NEAT1) in ccRCC progression. METHODS: We performed quantitative real time PCR and western blotting assays to measure mRNA and protein expression levels, respectively...
February 27, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28260918/long-noncoding-rna-neat1-promotes-cell-proliferation-and-invasion-by-regulating-hnrnp-a2-expression-in-hepatocellular-carcinoma-cells
#14
Yuanyi Mang, Li Li, Jianghua Ran, Shengning Zhang, Jing Liu, Laibang Li, Yiming Chen, Jian Liu, Yang Gao, Gang Ren
Growing evidence demonstrates that long noncoding RNAs (lncRNAs) are involved in the progression of various cancers, including hepatocellular carcinoma (HCC). The role of nuclear-enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, has not been fully explored in HCC. We aimed to determine the expression, roles and functional mechanisms of NEAT1 in the proliferation and invasion of HCC. Based on real-time polymerase chain reaction data, we suggest that NEAT1 is upregulated in HCC tissues compared with noncancerous liver tissues...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28255245/prognostic-role-of-long-noncoding-rna-neat1-in-various-carcinomas-a-meta-analysis
#15
Tao Chen, Hui Wang, Peng Yang, Zhen-Yu He
A number of studies have revealed that nuclear paraspeckle assembly transcript 1 (NEAT1), a long noncoding RNA (lncRNA), was aberrantly regulated in various cancers. High NEAT1 expression was associated with a poor prognosis and an increased risk of lymph node metastasis (LNM) in cancer patients. This meta-analysis was conducted to identify the potential value of NEAT1 as a biomarker for cancer prognosis. We searched the electronic databases PubMed, Web of Science, and China National Knowledge Infrastructure (up to November 13, 2016) to collect all relevant studies to explore the association between the expression of lncRNA NEAT1 and overall survival (OS) and LNM...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28239820/long-non-coding-rna-neat1-acts-as-oncogene-in-nsclc-by-regulating-the-wnt-signaling-pathway
#16
S-J Sun, Q Lin, J-X Ma, W-W Shi, B Yang, F Li
OBJECTIVE: The present study aimed to explore the role of long non-coding RNA NEAT1 (NEAT1) in mediating non-small cell lung cancer (NSCLC) cell migration and invasion, as well as the underlying regulatory mechanisms. PATIENTS AND METHODS: The NEAT1 expression in NSCLC tissues and cell lines was measured using reverse transcription‑quantitative polymerase chain reaction (RT-qPCR). The relationships between NEAT1 expression and clinicopathological parameters were examined by chi-square test...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28202761/the-long-noncoding-rna-neat1-exerts-anti-hantaviral-effects-by-acting-as-a-positive-feedback-for-rig-i-signaling
#17
Hongwei Ma, Peijun Han, Wei Ye, Hesong Chen, Xuyang Zheng, Linfeng Cheng, Liang Zhang, Lan Yu, Xing'an Wu, Zhikai Xu, Yingfeng Lei, Fanglin Zhang
Hantavirus infection, which causes zoonotic diseases with a high mortality rate in humans, has long been a global public health concern. Over the past decades, accumulating evidence suggests that long noncoding RNAs (lncRNAs) play key regulatory roles in innate immunity. However, the involvement of host lncRNAs in hantaviral control remains uncharacterized. In this study, we identified the lncRNA, NEAT1, as a vital antiviral modulator. NEAT1 was dramatically upregulated after Hantaan virus (HTNV) infection, whereas its downregulation in vitro or in vivo delayed host innate immune responses and aggravated HTNV replication...
February 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28185956/long-non-coding-rna-neat1-regulates-permeability-of-the-blood-tumor-barrier-via-mir-181d-5p-mediated-expression-changes-in-zo-1-occludin-and-claudin-5
#18
Junqing Guo, Heng Cai, Jun Ma, Xiaobai Liu, Yunhui Liu, Jian Zheng, Zhongyou Que, Wei Gong, Yana Gao, Wei Tao, Yixue Xue
The blood-tumor barrier (BTB) constitutes an efficient organization of tight junctions that limits the delivery of chemotherapeutic drugs to brain tumor tissues and impacts the treatment of glioma. Long non-coding RNAs (lncRNAs) are non-protein coding RNAs regulating gene expression, some lncRNAs play a crucial role in BTB permeability. However, the function of lncRNAs in BTB permeability is still largely unclear. Here, we have identified lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1), was remarkably up-regulated in glioma endothelial cells (GECs) obtained from an in vitro BTB model...
February 6, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28132817/systematic-mapping-of-rna-chromatin-interactions-in%C3%A2-vivo
#19
Bharat Sridhar, Marcelo Rivas-Astroza, Tri C Nguyen, Weizhong Chen, Zhangming Yan, Xiaoyi Cao, Lucie Hebert, Sheng Zhong
RNA molecules can attach to chromatin. It remains difficult to know what RNAs are associated with chromatin and where the genomic target loci of these RNAs are. Here, we present MARGI (mapping RNA-genome interactions), a technology to massively reveal native RNA-chromatin interactions from unperturbed cells. The gist of this technology is to ligate chromatin-associated RNAs (caRNAs) with their target genomic sequences by proximity ligation, forming RNA-DNA chimeric sequences, which are converted to a sequencing library for paired-end sequencing...
February 20, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28128360/discovery-of-cancer-driver-long-noncoding-rnas-across-1112-tumour-genomes-new-candidates-and-distinguishing-features
#20
Andrés Lanzós, Joana Carlevaro-Fita, Loris Mularoni, Ferran Reverter, Emilio Palumbo, Roderic Guigó, Rory Johnson
Long noncoding RNAs (lncRNAs) represent a vast unexplored genetic space that may hold missing drivers of tumourigenesis, but few such "driver lncRNAs" are known. Until now, they have been discovered through changes in expression, leading to problems in distinguishing between causative roles and passenger effects. We here present a different approach for driver lncRNA discovery using mutational patterns in tumour DNA. Our pipeline, ExInAtor, identifies genes with excess load of somatic single nucleotide variants (SNVs) across panels of tumour genomes...
January 27, 2017: Scientific Reports
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