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Carmen Adriaens, Jean-Christophe Marine
No abstract text is available yet for this article.
October 4, 2016: Cell Cycle
Aparna Anantharaman, Mahdieh Jadaliha, Vidisha Tripathi, Shinichi Nakagawa, Tetsuro Hirose, Michael F Jantsch, Supriya G Prasanth, Kannanganattu V Prasanth
Paraspeckles are sub-nuclear domains that are nucleated by long noncoding RNA Neat1. While interaction of protein components of paraspeckles and Neat1 is understood, there is limited information on the interaction of non-structural RNA components with paraspeckles. Here, by varying paraspeckle number and size, we investigate how paraspeckles influence the nuclear organization of their non-structural RNA component Ctn RNA. Our results show that Ctn RNA remains nuclear-retained in the absence of intact paraspeckles, suggesting that they do not regulate nuclear retention of Ctn RNA...
September 26, 2016: Scientific Reports
Zhiguo Li, Dianjun Wei, Chao Yang, Honggang Sun, Tao Lu, Defeng Kuang
Long noncoding RNAs (lncRNAs) are emerging as important modulators in the biological processes and tumorigenesis. However, whether lncRNAs are involved in endometrial endometrioid adenocarcinoma (EEC) remains unclear. In the present study, we explored the expression pattern, clinical significance and biological function of nuclear enriched abundant transcript 1 (NEAT1) in EEC. The expression levels of NEAT1 were elevated in EEC tissues and cell lines, and higher expression levels of NEAT1 were positively correlated with FIGO stage and lymph node metastasis...
September 21, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jason A West, Mari Mito, Satoshi Kurosaka, Toru Takumi, Chiharu Tanegashima, Takeshi Chujo, Kaori Yanaka, Robert E Kingston, Tetsuro Hirose, Charles Bond, Archa Fox, Shinichi Nakagawa
Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle proteins are found within different layers along the radially arranged bundles of Neat1 transcripts, forming a characteristic core-shell spheroidal structure...
September 26, 2016: Journal of Cell Biology
Shi-Bin Hu, Run-Wen Yao, Ling-Ling Chen
The nuclear body paraspeckle is built on the lncRNA Neat1 and plays important roles in gene regulation. In this issue, West et al. (2016. J. Cell Biol use super-resolution structured illumination microscopy to show that paraspeckles are organized in a core-shell spheroidal structure composed of Neat1 and seven proteins.
September 26, 2016: Journal of Cell Biology
Sha Ni, Xiaoyu Zhao, Ling Ouyang
Researchers have recently demonstrated the key role of long non-coding RNAs (lncRNAs) in regulating embryogenesis and gene expression. However, the exact mechanism used by lncRNAs in carcinogenesis is still unclear. In particular, studies regarding the role of lncRNAs in vulvar squamous cell carcinomas (VSCCs) are limited. Using microarray analysis, the genome-wide expression profile of lncRNAs was investigated in four paired VSCCs and adjacent normal vulvar tissues. Accordingly, several novel lncRNA candidates (HOAIR, MALAT1, MEG3, NEAT1, MIR31HG and LINC00478) were chosen for further study and real-time reverse transcription PCR (RT-PCR) was used to confirm the expression levels among 35 tissue samples...
September 8, 2016: Oncology Reports
Z-J Chen, Z Zhang, B-B Xie, H-Y Zhang
OBJECTIVE: The purpose of the present study was to examine the expression levels of lncRNA NEAT1 in fresh ovarian cancer (OC) tissue and matched adjacent normal tissue specimens, and investigate the clinical significance of NEAT1 in OC. PATIENTS AND METHODS: NEAT1 levels in OC tissues and matched adjacent normal tissue specimens were tested by qRT-PCR. Then, statistical analysis was performed to explore the associations of NEAT1 expression with the clinical features and the prognosis of OC...
August 2016: European Review for Medical and Pharmacological Sciences
Wei Gong, Jian Zheng, Xiaobai Liu, Jun Ma, Yunhui Liu, Yixue Xue
Nuclear paraspeckle assembly transcript 1 (NEAT1), a long non-coding RNA, promotes oncogenesis in various tumors, including human gliomas. Herein, we studied the expression and function of NEAT1 in glioma stem cells (GSCs). Quantitative real-time PCR demonstrated that NEAT1 was upregulated in GSCs. NEAT1 knockdown inhibited GSC cell proliferation, migration and invasion and promoted GSC apoptosis. A potential binding region between NEAT1 and microRNA let-7e was confirmed by dual-luciferase assays. Upregulation of NEAT1 reduced the expression of let-7e, and there was reciprocal repression between NEAT1 and let-7e in an Argonaute 2-dependent manner...
August 19, 2016: Oncotarget
Pang-Kuo Lo, Yongshu Zhang, Benjamin Wolfson, Ramkishore Gernapudi, Yuan Yao, Nadire Duru, Qun Zhou
Dysregulation of long non-codng RNA (lncRNA) expression has been found to contribute to tumorigenesis. However, the roles of lncRNAs in BRCA1-related breast cancer remain largely unknown. In this study, we delineate the role of the novel BRCA1/lncRNA NEAT1 signaling axis in breast tumorigenesis. BRCA1 inhibits NEAT1 expression potentially through binding to its genomic binding site upstream of the NEAT1 gene. BRCA1 deficiency in human normal/cancerous breast cells and mouse mammary glands leads to NEAT1 overexpression...
August 18, 2016: Oncotarget
A Rasim Barutcu, Deli Hong, Bryan R Lajoie, Rachel Patton McCord, Andre J van Wijnen, Jane B Lian, Janet L Stein, Job Dekker, Anthony N Imbalzano, Gary S Stein
RUNX1 is a transcription factor functioning both as an oncogene and a tumor suppressor in breast cancer. RUNX1 alters chromatin structure in cooperation with chromatin modifier and remodeling enzymes. In this study, we examined the relationship between RUNX1-mediated transcription and genome organization. We characterized genome-wide RUNX1 localization and performed RNA-seq and Hi-C in RUNX1-depleted and control MCF-7 breast cancer cells. RNA-seq analysis showed that RUNX1 depletion led to up-regulation of genes associated with chromatin structure and down-regulation of genes related to extracellular matrix biology, as well as NEAT1 and MALAT1 lncRNAs...
August 9, 2016: Biochimica et Biophysica Acta
Caihua Gao, Jianying Zhang, Qingyan Wang, Chunhua Ren
Leukemia is a heterogeneous clonal disorder in which early hematopoietic cells fail to differentiate and do not undergo programmed cell death or apoptosis. Less than one-third of adult patients with leukemia are managed using current therapies due to the emergence of multidrug resistance (MDR), emphasizing the need for newer and more robust approaches. Recent reports have suggested that long non-coding RNAs (lncRNAs) contribute to selective gene expression and, hence, could be manipulated effectively to halt the progression of cancer...
August 2016: Oncology Letters
Manon Torres, Denis Becquet, Marie-Pierre Blanchard, Séverine Guillen, Bénédicte Boyer, Mathias Moreno, Jean-Louis Franc, Anne-Marie François-Bellan
Paraspeckles are nuclear bodies form around the long non-coding RNA, Neat1, and RNA-binding proteins. While their role is not fully understood, they are believed to control gene expression at a post-transcriptional level by means of the nuclear retention of mRNA containing in their 3'-UTR inverted repeats of Alu sequences (IRAlu). In this study, we found that, in pituitary cells, all components of paraspeckles including four major proteins and Neat1 displayed a circadian expression pattern. Furthermore the insertion of IRAlu at the 3'-UTR of the EGFP cDNA led to a rhythmic circadian nuclear retention of the egfp mRNA that was lost when paraspeckles were disrupted whereas insertion of a single antisense Alu had only a weak effect...
2016: ELife
Chen XianGuo, Hao ZongYao, Zhou Jun, Fan Song, Luo GuangYue, Zhang LiGang, Zhang KaiPing, Zhang YangYang, Liang ChaoZhao
Bladder cancer was the most important reason of cancer-related death around the world, and urgently requires new therapeutic methods targeting the malignant tumor. There are many reports that the long noncoding RNAs are participated in different cancers, however, limited data are found between the long noncoding RNAs and bladder cancer. Previous studies have indicated that lncRNAs play vital roles in gene regulatory processes which could influence carcinoma progression.It is well known that lncRNAs can't code proteins, however, controlling transcription was found in the life process...
June 15, 2016: Oncotarget
Carmen Adriaens, Laura Standaert, Jasmine Barra, Mathilde Latil, Annelien Verfaillie, Peter Kalev, Bram Boeckx, Paul W G Wijnhoven, Enrico Radaelli, William Vermi, Eleonora Leucci, Gaëlle Lapouge, Benjamin Beck, Joost van den Oord, Shinichi Nakagawa, Tetsuro Hirose, Anna A Sablina, Diether Lambrechts, Stein Aerts, Cédric Blanpain, Jean-Christophe Marine
In a search for mediators of the p53 tumor suppressor pathway, which induces pleiotropic and often antagonistic cellular responses, we identified the long noncoding RNA (lncRNA) NEAT1. NEAT1 is an essential architectural component of paraspeckle nuclear bodies, whose pathophysiological relevance remains unclear. Activation of p53, pharmacologically or by oncogene-induced replication stress, stimulated the formation of paraspeckles in mouse and human cells. Silencing Neat1 expression in mice, which prevents paraspeckle formation, sensitized preneoplastic cells to DNA-damage-induced cell death and impaired skin tumorigenesis...
August 2016: Nature Medicine
Chengcao Sun, Shujun Li, Feng Zhang, Yongyong Xi, Liang Wang, Yongyi Bi, Dejia Li
Recently, the long non-coding RNA (lncRNA) NEAT1 has been identified as an oncogenic gene in multiple cancer types and elevated expression of NEAT1 was tightly linked to tumorigenesis and cancer progression. However, the molecular basis for this observation has not been characterized in progression of non-small cell lung cancer (NSCLC). In our studies, we identified NEAT1 was highly expressed in patients with NSCLC and was a novel regulator of NSCLC progression. Patients whose tumors had high NEAT1 expression had a shorter overall survival than patients whose tumors had low NEAT1 expression...
June 16, 2016: Oncotarget
Paola Riva, Antonia Ratti, Marco Venturin
BACKGROUND: Long-non-coding RNAs (lncRNAs), RNA molecules longer than 200 nucleotides, have been involved in several biological processes and in a growing number of diseases, controlling gene transcription, pre-mRNA processing, the transport of mature mRNAs to specific cellular compartments, the regulation of mRNA stability, protein translation and turnover. The fundamental role of lncRNAs in central nervous system (CNS) is becoming increasingly evident. LncRNAs are abundantly expressed in mammalian CNS in a specific spatio-temporal manner allowing a quick response to environmental/molecular changes...
June 22, 2016: Current Alzheimer Research
Guillaume Cornelis, Sylvie Souquere, Cécile Vernochet, Thierry Heidmann, Gérard Pierron
Long non-coding RNAs (lncRNAs) are widely expressed and play various roles in cell homeostasis. However, because of their low conservation at the sequence level, recapitulating lncRNA evolutionary history is often challenging. While performing an ultrastructural analysis of viral particles present in uterine glands of gestating opossum females, we serendipitously noticed the presence of numerous structures similar to paraspeckles, nuclear bodies which in human and mouse cells are assembled around an architectural NEAT1/MENϵ/β lncRNA...
September 2016: RNA Biology
Lauren C Butchart, Archa Fox, Tea Shavlakadze, Miranda D Grounds
Post-natal growth of skeletal muscle is a dynamic process involving proliferation and fusion of myoblasts with elongating myofibres (hyperplasia of myonuclei) until 3 weeks post-natally in mice, with ongoing differentiation and further increases in myofibre size mostly by hypertrophy until about 12 weeks of age. The expression of mRNAs that control these events are well described, but little is known about the in vivo roles of non-coding RNAs (ncRNAs), including both microRNAs (miRNAs) and the lesser-studied long non-coding RNAs (lncRNAs)...
June 9, 2016: Differentiation; Research in Biological Diversity
Pan Jiang, Xiaoyue Wu, Xuemin Wang, Wenbin Huang, Qing Feng
Platinum-based drugs are the firstline of treatment for non-small cell lung cancer (NSCLC), but resistance to these drugs is a major obstacle to effective chemotherapy. Our previous study revealed that the green tea polyphenol, EGCG, induced cisplatin transporter CTR1 (copper transporter 1) and enhanced cisplatin sensitivity in ovarian cancer. In this study, we found that EGCG upregulated CTR1 and increased platinum accumulation in NSCLC (A549, H460 and H1299) cells, cDDP-resistant A549 cells and a nude mouse xenograft model...
May 30, 2016: Oncotarget
Marta Melé, John L Rinn
There is growing evidence that transcription and nuclear organization are tightly linked. Yet, whether transcription of thousands of long noncoding RNAs (lncRNAs) could play a role in this packaging process remains elusive. Although some lncRNAs have been found to have clear roles in nuclear architecture (e.g., FIRRE, NEAT1, XIST, and others), the vast majority remain poorly understood. In this Perspective, we highlight how the act of transcription can affect nuclear architecture. We synthesize several recent findings into a proposed model where the transcription of lncRNAs can serve as guide-posts for shaping genome organization...
June 2, 2016: Molecular Cell
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