Luciano F Huergo, Khaled A Selim, Marcelo S Conzentino, Edileusa C M Gerhardt, Adrian R S Santos, Berenike Wagner, Janette T Alford, Nelli Deobald, Fabio O Pedrosa, Emanuel M de Souza, Meri B Nogueira, Sônia M Raboni, Dênio Souto, Fabiane G M Rego, Dalila L Zanette, Mateus N Aoki, Jeanine M Nardin, Bruna Fornazari, Hugo M P Morales, Vânia A Borges, Annika Nelde, Juliane S Walz, Matthias Becker, Nicole Schneiderhan-Marra, Ulrich Rothbauer, Rodrigo A Reis, Karl Forchhammer
Immunological methods to detect SARS-CoV-2 seroconversion in humans are important to track COVID-19 cases and the humoral response to SARS-CoV-2 infections and immunization to future vaccines. The aim of this work was to develop a simple chromogenic magnetic bead-based immunoassay which allows rapid, inexpensive, and quantitative detection of human antibodies against SARS-CoV-2 in serum, plasma, or blood. Recombinant 6xHis-tagged SARS-CoV-2 Nucleocapsid protein was mobilized on the surface of Ni2+ magnetic beads and challenged with serum or blood samples obtained from controls or COVID-19 cases...
January 26, 2021: ACS Sensors