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https://www.readbyqxmd.com/read/29220867/sarcomeric-tpm3%C3%AE-in-developing-chicken
#1
Syamalima Dube, Lynn Abbott, Samender Randhawa, Yingli Fan, Jushuo Wang, Jean M Sanger, Joseph W Sanger, Bernard J Poiesz, Dipak K Dube
Cloning and sequencing of various tropomyosin isoforms expressed in chickens have been described since the early 1980s. However, to the best of our knowledge, this is the first report on the molecular characterization and the expression of the sarcomeric isoform of the TPM3 gene in cardiac and skeletal muscles from developing as well as adult chickens. Expression of TPM3α was performed by conventional RT-PCR as well as qRT-PCR using relative expression (by ΔCT as well as ΔΔCT methods) and by determining absolute copy number...
December 8, 2017: Cytoskeleton
https://www.readbyqxmd.com/read/29217433/hypertrophic-cardiomyopathy-linked-mutation-in-troponin-t-causes-myofibrillar-disarray-and-pro-arrhythmic-action-potential-changes-in-human-ipsc-cardiomyocytes
#2
Lili Wang, Kyungsoo Kim, Shan Parikh, Adrian Gabriel Cadar, Kevin R Bersell, Huan He, Jose R Pinto, Dmytro O Kryshtal, Bjorn C Knollmann
BACKGROUND: Mutations in cardiac troponin T (TnT) are linked to increased risk of ventricular arrhythmia and sudden death despite causing little to no cardiac hypertrophy. Studies in mice suggest that the hypertrophic cardiomyopathy (HCM)-associated TnT-I79N mutation increases myofilament Ca sensitivity and is arrhythmogenic, but whether findings from mice translate to human cardiomyocyte electrophysiology is not known. OBJECTIVES: To study the effects of the TnT-I79N mutation in human cardiomyocytes...
December 4, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29214232/hybrid-protein-glycosaminoglycan-hydrogels-promote-chondrogenic-stem-cell-differentiation
#3
Vladimíra Moulisová, Sara Poveda-Reyes, Esther Sanmartín-Masiá, Luis Quintanilla-Sierra, Manuel Salmerón-Sánchez, Gloria Gallego Ferrer
Gelatin-hyaluronic acid (Gel-HA) hybrid hydrogels have been proposed as matrices for tissue engineering because of their ability to mimic the architecture of the extracellular matrix. Our aim was to explore whether tyramine conjugates of Gel and HA, producing injectable hydrogels, are able to induce a particular phenotype of encapsulated human mesenchymal stem cells without the need for growth factors. While pure Gel allowed good cell adhesion without remarkable differentiation and pure HA triggered chondrogenic differentiation without cell spreading, the hybrids, especially those rich in HA, promoted chondrogenic differentiation as well as cell proliferation and adhesion...
November 30, 2017: ACS Omega
https://www.readbyqxmd.com/read/29208528/heterochromatin-protein-hp-1%C3%AE-is-not-only-in-the-nucleus-but-also-in-the-cytoplasm-interacting-with-actin-in-both-cell-compartments
#4
Nancy L Charó, Natalia M Galigniana, Graciela Piwien-Pilipuk
Confocal and electron microscopy images, and WB analysis of cellular fractions revealed that HP1γ is in the nucleus but also in the cytoplasm of C2C12 myoblasts, myotubes, skeletal and cardiac muscles, N2a, HeLa and HEK293T cells. Signal specificity was tested with different antibodies and by HP1γ knockdown. Leptomycin B treatment of myoblasts increased nuclear HP1γ, suggesting that its nuclear export is Crm-1-dependent. HP1γ exhibited a filamentous pattern of staining partially co-localizing with actin in the cytoplasm of myotubes and myofibrils...
December 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29207039/effective-component-of-salvia%C3%A2-miltiorrhiza-in-promoting-cardiomyogenic-differentiation-of-human-placenta%C3%A2-derived-mesenchymal-stem-cells
#5
Kun Li, Jieqiong Song, Qian Zhao, Bo Wang, Yunli Zhang, Xuezhe Wang, Tiantian Tang, Shizheng Li
Our previous study indicated that Salvia miltiorrhiza (SM) induced human placenta‑derived mesenchymal stem cells (hPDMSCs) to differentiate into cardiomyocytes, however, the effective component of SM in promoting cardiomyogenic differentiation remained to be elucidated. In the present study, the most commonly examined components of SM, including danshensu, salvianolic acid B, protocatechuic aldehyde, tanshinone I (TS I), TS IIA and cryptotanshinone, were used to determine the effective components of SM in promoting cardiomyogenic differentiation...
November 28, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29206857/z-disc-protein-chapb-induces-cardiomyopathy-and-contractile-dysfunction-in-the-postnatal-heart
#6
Willemijn van Eldik, Brigit den Adel, Jantine Monshouwer-Kloots, Daniela Salvatori, Saskia Maas, Ingeborg van der Made, Esther E Creemers, Derk Frank, Norbert Frey, Nicky Boontje, Jolanda van der Velden, Paul Steendijk, Christine Mummery, Robert Passier, Abdelaziz Beqqali
AIMS: The Z-disc is a crucial structure of the sarcomere and is implicated in mechanosensation/transduction. Dysregulation of Z-disc proteins often result in cardiomyopathy. We have previously shown that the Z-disc protein Cytoskeletal Heart-enriched Actin-associated Protein (CHAP) is essential for cardiac and skeletal muscle development. Furthermore, the CHAP gene has been associated with atrial fibrillation in humans. Here, we studied the misregulated expression of CHAP isoforms in heart disease...
2017: PloS One
https://www.readbyqxmd.com/read/29203715/the-role-of-heat-shock-proteins-and-co-chaperones-in-heart-failure
#7
REVIEW
Mark J Ranek, Marisa J Stachowski, Jonathan A Kirk, Monte S Willis
The ongoing contractile and metabolic demands of the heart require a tight control over protein quality control, including the maintenance of protein folding, turnover and synthesis. In heart disease, increases in mechanical and oxidative stresses, post-translational modifications (e.g., phosphorylation), for example, decrease protein stability to favour misfolding in myocardial infarction, heart failure or ageing. These misfolded proteins are toxic to cardiomyocytes, directly contributing to the common accumulation found in human heart failure...
January 19, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29199018/chemical-crosslinking-mass-spectrometry-analysis-of-protein-conformations-and-supercomplexes-in-heart-tissue
#8
Juan D Chavez, Chi Fung Lee, Arianne Caudal, Andrew Keller, Rong Tian, James E Bruce
While modern structural biology technologies have greatly expanded the size and type of protein complexes that can now be studied, the ability to derive large-scale structural information on proteins and complexes as they exist within tissues is practically nonexistent. Here, we demonstrate the application of crosslinking mass spectrometry to identify protein structural features and interactions in tissue samples, providing systems structural biology insight into protein complexes as they exist in the mouse heart...
November 24, 2017: Cell Systems
https://www.readbyqxmd.com/read/29196542/cardiac-myosin-binding-protein-c-is-a-novel-marker-of-myocardial-injury-and-fibrosis-in-aortic-stenosis
#9
Atul Anand, Calvin Chin, Anoop S V Shah, Jacek Kwieciński, Alex Vesey, Joanna Cowell, Ekkehard Weber, Thomas Kaier, David E Newby, Marc Dweck, Michael S Marber, Nicholas L Mills
OBJECTIVE: Cardiac myosin-binding protein C (cMyC) is an abundant sarcomeric protein and novel highly specific marker of myocardial injury. Myocyte death characterises the transition from hypertrophy to replacement myocardial fibrosis in advanced aortic stenosis. We hypothesised that serum cMyC concentrations would be associated with cardiac structure and outcomes in patients with aortic stenosis. METHODS: cMyC was measured in two cohorts in which serum had previously been prospectively collected: a mechanism cohort of patients with aortic stenosis (n=161) and healthy controls (n=46) who underwent cardiac MRI, and an outcome cohort with aortic stenosis (n=104) followed for a median of 11...
December 1, 2017: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/29196500/reduced-prostaglandin-i2-signaling-in-arid5b-primary-skeletal-muscle-cells-attenuates-myogenesis
#10
Jennifer Murray, Robert H Whitson, Keiichi Itakura
The AT-rich interaction domain (ARID) family of proteins regulates gene expression, development, and differentiation. Although Arid5b has important functions in adipogenesis and chondrogenesis, the role of Arid5b in skeletal muscle myogenesis has not been investigated. Therefore, we isolated primary skeletal muscle cells from Arid5b+/+ and Arid5b-/- mice and characterized differentiation in these cells. We found that Arid5b-/- primary skeletal muscle cells showed differentiation defects and impaired sarcomeric assembly...
December 1, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29190771/synergistic-role-of-5-azacytidine-and-ascorbic-acid-in-directing-cardiosphere-derived-cells-to-cardiomyocytes-in-vitro-by-downregulating-wnt-signaling-pathway-via-phosphorylation-of-%C3%AE-catenin
#11
Reddy Sailaja Mundre, Pavani Koka, Prakash Dhanaraj, Nitin Khatri, Sanjana Vig, Yamini Chandramohan, Anuradha Dhanasekaran
BACKGROUND: Cardiosphere derived cells (CDCs) represent a valuable source in stem cell based therapy for cardiovascular diseases, yet poor differentiation rate hinders the transplantation efficiency. The aim of this study is to check the ability of 5-Azacytidine (Aza) alone and in combination with ascorbic acid (Aza+AA) in delineating CDCs to cardiomyogenesis and the underlying Wnt signaling mechanism in induced differentiation. METHODS: CDCs were treated with Aza and Aza+AA for a period of 14 days to examine the expression of cardiac specific markers and Wnt downstream regulators by immunofluorescence, real time PCR and western blot...
2017: PloS One
https://www.readbyqxmd.com/read/29187535/transient-receptor-potential-channel-6-regulates-abnormal-cardiac-s-nitrosylation-in-duchenne-muscular-dystrophy
#12
Heaseung Sophia Chung, Grace E Kim, Ronald J Holewinski, Vidya Venkatraman, Guangshuo Zhu, Djahida Bedja, David A Kass, Jennifer E Van Eyk
Duchenne muscular dystrophy (DMD) is an X-linked disorder with dystrophin loss that results in skeletal and cardiac muscle weakening and early death. Loss of the dystrophin-sarcoglycan complex delocalizes nitric oxide synthase (NOS) to alter its signaling, and augments mechanosensitive intracellular Ca2+ influx. The latter has been coupled to hyperactivation of the nonselective cation channel, transient receptor potential canonical channel 6 (Trpc6), in isolated myocytes. As Ca2+ also activates NOS, we hypothesized that Trpc6 would help to mediate nitric oxide (NO) dysregulation and that this would be manifest in increased myocardial S-nitrosylation, a posttranslational modification increasingly implicated in neurodegenerative, inflammatory, and muscle disease...
November 29, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29183906/cardiac-enriched-baf-chromatin-remodeling-complex-subunit-baf60c-regulates-gene-expression-programs-essential-for-heart-development-and-function
#13
Xin Sun, Swetansu K Hota, Yu-Qing Zhou, Stefanie Novak, Dario Miguel-Perez, Danos Christodoulou, Christine E Seidman, J G Seidman, Carol C Gregorio, R Mark Henkelman, Janet Rossant, Benoit G Bruneau
How chromatin-remodeling complexes modulate gene networks to control organ-specific properties is not well understood. For example, Baf60c (Smarcd3) encodes a cardiac-enriched subunit of the SWI/SNF-like BAF chromatin complex, but its role in heart development is not fully understood. We found that constitutive loss of Baf60c leads to embryonic cardiac hypoplasia and pronounced cardiac dysfunction. Conditional deletion of Baf60c in cardiomyocytes resulted in postnatal dilated cardiomyopathy with impaired contractile function...
November 28, 2017: Biology Open
https://www.readbyqxmd.com/read/29180479/influence-of-residual-force-enhancement-and-elongation-of-attached-cross-bridges-on-stretch-shortening-cycle-in-skinned-muscle-fibers
#14
Atsuki Fukutani, Venus Joumaa, Walter Herzog
Increased muscle force during stretch-shortening cycles (SSCs) has been widely examined. However, the mechanisms causing increased muscle force in SSCs remain unknown. The purpose of this study was to determine the influence of residual force enhancement and elongation of attached cross-bridges on the work enhancement in SSCs. For the Control condition, skinned rabbit soleus fibers were elongated passively from an average sarcomere length of 2.4 to 3.0 μm, activated and then actively shortened to 2.4 μm...
November 2017: Physiological Reports
https://www.readbyqxmd.com/read/29177058/biomarkers-of-cardiovascular-stress-and-fibrosis-in-preclinical-hypertrophic-cardiomyopathy
#15
Jennifer E Ho, Ling Shi, Sharlene M Day, Steven D Colan, Mark W Russell, Jeffrey A Towbin, Mark V Sherrid, Charles E Canter, John Lynn Jefferies, Anne Murphy, Matthew Taylor, Luisa Mestroni, Allison L Cirino, Lynn A Sleeper, Peter Jarolim, Begoña Lopez, Arantxa Gonzalez, Javier Diez, E John Orav, Carolyn Y Ho
Objective: Sarcomeric gene mutation carriers without overt left ventricular hypertrophy (G+/LVH-) can harbour subclinical changes in cardiovascular structure and function that precede the development of hypertrophic cardiomyopathy (HCM). We sought to investigate if circulating biomarkers of cardiovascular stress and collagen metabolism among G+/LVH- individuals, measured at rest and following exercise provocation, yield further insights into the underlying biology of HCM. Methods: We studied 76 individuals with overt HCM, 50 G+/LVH- individuals and 41 genotype-negative related controls enrolled in a cross-sectional, multicentre observational study (HCMNet)...
2017: Open Heart
https://www.readbyqxmd.com/read/29175173/diagnostic-and-clinical-significance-of-the-titin-fragment-in-urine-of-duchenne-muscular-dystrophy-patients
#16
Hiroyuki Awano, Masaaki Matsumoto, Masashi Nagai, Taku Shirakawa, Nobuhiro Maruyama, Kazumoto Iijima, Yo-Ichi Nabeshima, Masafumi Matsuo
Duchenne muscular dystrophy (DMD) is a fatal progressive muscle wasting disease of childhood. Titin in sarcomere is digested by calcium dependent protease. To explore muscle damage in DMD, the urinary concentrations of the N-terminal fragment of titin were determined using a newly developed enzyme linked immune sorbent assay kit. The urinary titin concentrations were normalized to creatinine (Cr). A total of 145 urine samples were obtained at a single Japanese hospital from 113 DMD patients aged 3-29years. Normalized urinary titin concentration was 965...
November 23, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29169900/cardiotonic-actions-of-quercetin-and-its-metabolite-tamarixetin-through-a-digitalis-like-enhancement-of-ca-2-transients
#17
Kengo Hayamizu, Sachio Morimoto, Miki Nonaka, Sumio Hoka, Toshiyuki Sasaguri
The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca(2+) transients (CaT) of mouse single cardiomyocytes were simultaneously measured and compared with those of isoproterenol and digoxin. Furthermore, cardiac function and plasma concentrations were analyzed after bolus intravenous administration of QCT in mice. QCT and its metabolite, tamarixetin, as well as isoproterenol and digoxin, enhanced the contraction and CaT of cardiomyocytes...
November 20, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29169065/can-postmortem-proteolysis-explain-tenderness-differences-in-various-bovine-muscles
#18
Eva Veiseth-Kent, Mona E Pedersen, Sissel B Rønning, Rune Rødbotten
This study investigated the relationship between postmortem proteolysis, muscle pH decline, sarcomere length (SL), intramuscular fat (IMF) and Warner-Bratzler shear force (WBSF) in four bovine muscles (biceps femoris (BF), infraspinatus (IS), longissimus lumborum (LL), psoas major (PM). The WBSF was low in BF, IS and PM, while LL had a higher value (P<0.001), but still considered as tender. The PM had fastest pH decline (P<0.001), ultimate pH was lowest in LL and PM and highest for IS (P<0.001), sarcomeres were longest for PM and shortest for BF and LL (P<0...
November 8, 2017: Meat Science
https://www.readbyqxmd.com/read/29168801/the-histone-code-reader-spin1-controls-skeletal-muscle-development
#19
Holger Greschik, Delphine Duteil, Nadia Messaddeq, Dominica Willmann, Laura Arrigoni, Manuela Sum, Manfred Jung, Daniel Metzger, Thomas Manke, Thomas Günther, Roland Schüle
While several studies correlated increased expression of the histone code reader Spin1 with tumor formation or growth, little is known about physiological functions of the protein. We generated Spin1(M5) mice with ablation of Spin1 in myoblast precursors using the Myf5-Cre deleter strain. Most Spin1(M5) mice die shortly after birth displaying severe sarcomere disorganization and necrosis. Surviving Spin1(M5) mice are growth-retarded and exhibit the most prominent defects in soleus, tibialis anterior, and diaphragm muscle...
November 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29167554/phenotypic-expression-of-a-novel-desmin-gene-mutation-hypertrophic-cardiomyopathy-followed-by-systemic-myopathy
#20
Haruhito Harada, Takeharu Hayashi, Hirofumi Nishi, Ken Kusaba, Yoshinori Koga, Yasutoshi Koga, Ikuya Nonaka, Akinori Kimura
Hypertrophic cardiomyopathy is a heterogeneous disease caused by gene mutations. Most of the disease-causing mutations were found in the genes for sarcomeric proteins, but there are several cases carrying mutations in genes for extra-sarcomeric cytoskeletons. Desmin is a member of extra-sarcomeric cytoskeletons and plays an important role in muscle contraction. Mutations in the desmin gene cause various type of general myopathy and/or cardiomyopathy, known as desmin-related myopathies. We identified a novel desmin missense mutation, Thr219Pro, in the homozygous state in a patient, who first manifested with hypertrophic cardiomyopathy and later progressed to general myopathy...
November 22, 2017: Journal of Human Genetics
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