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https://www.readbyqxmd.com/read/28938870/evaluation-of-prokaryotic-diversity-of-five-hot-springs-in-eritrea
#1
Amanuel M Ghilamicael, Nancy L M Budambula, Sylvester E Anami, Tadesse Mehari, Hamadi I Boga
BACKGROUND: Total community rDNA was used to determine the diversity of bacteria and archaea from water, wet sediment and microbial mats samples of hot springs in the Eastern lowlands of Eritrea. The temperatures of the springs range from 49.5 °C to 100 °C while pH levels varied from 6.97 to 7.54. Akwar and Maiwooi have high carbonate levels. The springs near the seashore, Garbanabra and Gelti, are more saline with higher levels of sodium and chlorides. Elegedi, situated in the Alid volcanic area, has the highest temperature, iron and sulfate concentrations...
September 22, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28936924/expression-of-cross-tolerance-to-a-wide-range-of-conditions-in-a-human-lung-cancer-cell-line-after-adaptation-to-nitric-oxide
#2
Zane Deliu, Timothy Tamas, Juel Chowdhury, Madeeha Aqil, Maaly Bassiony, James A Radosevich
Previously, we have shown that A549, a human lung adenocarcinoma, can be adapted to nitric oxide (NO(●)). NO(●) is a nitrogen-based free radical that is synthesized by a family of enzymes known as nitric oxide synthases. NO(●) has been shown to be overexpressed in patient populations of different cancers. In addition, it has been observed that patients who express high levels of nitric oxide synthases tend to have poorer clinical outcomes than those with low levels of expression. The original cell line A549 (parent) and the adapted A549-HNO (high nitric oxide) cell line serve as a useful model system to investigate the role of NO(●) in tumor progression and prognosis...
September 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28935959/the-crystal-structure-of-the-regulatory-domain-of-the-human-sodium-driven-chloride-bicarbonate-exchanger
#3
Carolina M Alvadia, Theis Sommer, Kaare Bjerregaard-Andersen, Helle Hasager Damkier, Michele Montrasio, Christian Aalkjaer, J Preben Morth
The sodium-driven chloride/bicarbonate exchanger (NDCBE) is essential for maintaining homeostatic pH in neurons. The crystal structure at 2.8 Å resolution of the regulatory N-terminal domain of human NDCBE represents the first crystal structure of an electroneutral sodium-bicarbonate cotransporter. The crystal structure forms an equivalent dimeric interface as observed for the cytoplasmic domain of Band 3, and thus establishes that the consensus motif VTVLP is the key minimal dimerization motif. The VTVLP motif is highly conserved and likely to be the physiologically relevant interface for all other members of the SLC4 family...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28933773/rapid-quantification-of-major-volatile-metabolites-in-fermented-food-and-beverages-using-gas-chromatography-mass-spectrometry
#4
Farhana R Pinu, Silas G Villas-Boas
Here we present a method for the accurate quantification of major volatile metabolites found in different food and beverages, including ethanol, acetic acid and other aroma compounds, using gas chromatography coupled to mass spectrometry (GC-MS). The method is combined with a simple sample preparation procedure using sodium chloride and anhydrous ethyl acetate. The GC-MS analysis was accomplished within 4.75 min, and over 80 features were detected, of which 40 were positively identified using an in-house and a commercialmass spectrometry (MS) library...
July 26, 2017: Metabolites
https://www.readbyqxmd.com/read/28931374/effects-of-dexmedetomidine-on-tnf-%C3%AE-and-interleukin-2-in-serum-of-rats-with-severe-craniocerebral-injury
#5
Wan-Wei Jiang, Qing-Hui Wang, Ya-Jing Liao, Pai Peng, Min Xu, Li-Xin Yin
BACKGROUND: Dexmedetomidine is a highly selective adrenergic receptor agonist, which has a dose-dependent sedative hypnotic effect. Furthermore, it also has pharmacological properties, and the ability to inhibit sympathetic activity and improve cardiovascular stability during an operation. However, its protective effect on patients with severe craniocerebral injury in the perioperative period remains unclear. METHOD: Eighty adult male SD rats were used and divided into two groups (n = 40, each group): dexmedetomidine injury group (experimental group), and sodium chloride injury group (control group)...
September 20, 2017: BMC Anesthesiology
https://www.readbyqxmd.com/read/28929938/fo-md-hybrid-system-for-real-dairy-wastewater-recycling
#6
Hongwei Song, Fang Xie, Weiwei Chen, Jinrong Liu
This study investigated a forward osmosis and membrane distillation (FO/MD) hybrid system for real dairy wastewater (DWW) recycling. Two types of FO membranes, cellulose triacetate-embedded polyester screen support (CTA-ES) and aquaporin inside (AQP), were employed. Sodium chloride was used as the draw solution. A cross-flow FO cell and an air gap membrane distillation module were established to conduct individual FO experiments and FO/MD experiments. From the experiments, an analysis of the water flux (Jw), reverse draw solute flux (Js), Js/Jw ratio and contaminant rejection was performed...
September 25, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28927409/biological-and-physicochemical-properties-of-biosurfactants-produced-by-lactobacillus-jensenii-p6a-and-lactobacillus-gasseri-p65
#7
I M C Morais, A L Cordeiro, G S Teixeira, V S Domingues, R M D Nardi, A S Monteiro, R J Alves, E P Siqueira, V L Santos
BACKGROUND: Lactobacillus species produce biosurfactants that can contribute to the bacteria's ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and the skin. Here, we described the biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. RESULTS: The biosurfactants produced by L. jensenii P6A and L. gasseri P65 reduced the water surface tension from 72 to 43...
September 19, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28924618/optical-studies-of-the-solution-phase-reduction-and-stabilization-of-indigo-tetrasulfonate-in-polyelectrolyte-complexes
#8
Becca Hoene, Dion Rivera
Ultraviolet-visible (UV-vis) and fluorescence spectroscopy have been used to characterize the polyelectrolyte complexes (PECs) formed when potassium indigo tetrasulfonate (ITS) interacts with poly diallydimethylammonium chloride (PDADMAC) through columbic attraction in the presence of the reducing agent sodium bisulfite, NaHSO3. The PDADMAC facilitates both the reduction of the ITS and the stabilization of the reduced state of the ITS in an atmospheric oxygen environment. Dilutions of the dye solution show that the PEC is stable to dilutions of at least 1 to 1000...
September 2017: Heliyon
https://www.readbyqxmd.com/read/28924220/towards-addressing-the-body-electrolyte-environment-via-sweat-analysis-pilocarpine-iontophoresis-supports-assessment-of-plasma-potassium-concentration
#9
Donato Vairo, Laurie Bruzzese, Marion Marlinge, Lea Fuster, Nabil Adjriou, Nathalie Kipson, Philippe Brunet, Jennifer Cautela, Yves Jammes, Giovanna Mottola, Stephane Burtey, Jean Ruf, Regis Guieu, Emmanuel Fenouillet
Electrolyte concentration in sweat depends on environmental context and physical condition but also on the pathophysiological status. Sweat analyzers may be therefore the future way for biological survey although how sweat electrolyte composition can reflect plasma composition remains unclear. We recruited 10 healthy subjects and 6 patients to have a broad range of plasma electrolyte concentrations (chloride, potassium and sodium) and pH. These variables were compared to those found in sweat produced following cycling exercise or pilocarpine iontophoresis, a condition compatible with operating a wearable device...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923771/differential-expression-of-putative-sodium-dependent-cation-chloride-cotransporters-in-aedes-aegypti
#10
Peter M Piermarini, Daniel C Akuma, John C Crow, Taylor L Jamil, Willa G Kerkhoff, Kenyatta C M F Viel, Christopher M Gillen
The yellow fever mosquito, Aedes aegypti, has three genes that code for proteins with sequence similarity to vertebrate Na(+)-K(+)-Cl(-) cotransporters (NKCCs) of the solute-linked carrier 12 superfamily of cation-chloride cotransporters (CCCs). We hypothesized that these mosquito NKCC orthologues have diverged to perform distinct roles in salt secretion and absorption. In phylogenetic analyses, one protein (aeNKCC1) groups with a Drosophila melanogaster NKCC that mediates salt secretion whereas two others (aeCCC2 and aeCCC3) group with a Drosophila transporter that is not functionally characterized...
September 18, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28922715/pm2-5-components-and-outpatient-visits-for-asthma-a-time-stratified-case-crossover-study-in-a-suburban-area
#11
Chau-Ren Jung, Li-Hao Young, Hui-Tsung Hsu, Ming-Yeng Lin, Yu-Cheng Chen, Bing-Fang Hwang, Perng-Jy Tsai
The effects of fine particles (PM2.5) on asthma have been widely confirmed by epidemiological research studies. However, a limited number of studies have investigated the relationship between exposure to different PM2.5 components and asthma. We characterized the PM2.5 components in a suburban site of central Taiwan and conducted a time-stratified case-crossover study to elaborate the effects of daily concentration of each PM2.5 component on asthma outpatient visits. We retrieved asthma outpatient claims for individuals less than 20 years old with a residential address in the Shalu district, Taiwan, from the National Health Insurance Research Database during 2000-2010...
September 12, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28922372/reduction-of-organelle-motility-by-removal-of-potassium-and-other-solutes
#12
John W Murray, David Yin, Allan W Wolkoff
There are surprisingly few studies that describe how the composition of cell culture medium may affect the trafficking of organelles. Here we utilize time lapse multi-channel fluorescent imaging to show that short term exposure of Huh-7 cells to medium lacking potassium, sodium, or chloride strongly reduces but does not eliminate the characteristic back and forth and cell-traversing movement of fluorescent EGF (FL-EGF) containing organelles. We focused on potassium because of its relatively low abundance in media and serum and its energy requiring accumulation into cells...
2017: PloS One
https://www.readbyqxmd.com/read/28918434/improved-dna-purification-with-quality-assurance-for-evaluation-of-the-microbial-genetic-content-of-constructed-wetlands
#13
Wenda Huang, Jingjing Guo, Ran Tao, Ying Man, Yunv Dai, Yang Yang
Efficient isolation of target DNA is a crucial first step of DNA-based metagenomic analyses of environmental samples. Insufficient quantity and purity of DNA isolated using commercial kits result in missing genetic information, especially for large-diameter substrates in constructed wetlands (CWs). Here, we addressed this problem by devising a cost-effective calcium chloride lysozyme-sodium dodecyl sulfate (SDS) method (CCLS), with key improvements in the steps of humic acid removal and cell lysis. The buffer comprising Tris, EDTA, Na2O2P7 and PVPP (TENP), and skim milk, could reduce adsorption between microorganisms and substrates, and calcium chloride precipitated and removed over 94% of humic acid...
September 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28915761/a-potential-model-for-sodium-chloride-solutions-based-on-the-tip4p-2005-water-model
#14
A L Benavides, M A Portillo, V C Chamorro, J R Espinosa, J L F Abascal, C Vega
Despite considerable efforts over more than two decades, our knowledge of the interactions in electrolyte solutions is not yet satisfactory. Not even one of the most simple and important aqueous solutions, NaCl(aq), escapes this assertion. A requisite for the development of a force field for any water solution is the availability of a good model for water. Despite the fact that TIP4P/2005 seems to fulfill the requirement, little work has been devoted to build a force field based on TIP4P/2005. In this work, we try to fill this gap for NaCl(aq)...
September 14, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28915474/simultaneous-removal-of-cadmium-zinc-and-manganese-using-electrocoagulation-influence-of-operating-parameters-and-electrolyte-nature
#15
Longqian Xu, Guangzhu Cao, Xiaojun Xu, Shuli Liu, Zhengyang Duan, Changhua He, Yao Wang, Qihua Huang
In the present study, the influence of operating parameters and electrolyte nature on the simultaneous removal of toxic metals (cadmium, zinc and manganese) from synthetic smelting wastewater by batch electrocoagulation was investigated. This wastewater contained high concentrations of anion-cation electrolytes. Results indicated that the efficiency of heavy metals removal can be enhanced by increasing the solution pH and current density. The Fe-Fe electrode combination is more effective than the other combinations (Al-Al, Al-Fe and Fe-Al)...
September 12, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28904420/oxygen-reduction-reaction-affected-by-sulfate-reducing-bacteria-different-roles-of-bacterial-cells-and-metabolites
#16
Jiajia Wu, Huaiqun Liu, Peng Wang, Dun Zhang, Yan Sun, Ee Li
Sulfate-reducing bacteria (SRB) were found to be capable of tolerating a certain amount of oxygen (O2), but how they affect oxygen reduction reaction (ORR) has not been clear. The present work investigated the impact of SRB on ORR in 3.5 wt% sodium chloride solution with the cyclic voltammetry method. The addition of SRB culture solution hampered both the reduction of O2 to superoxide (O2(·-)) and hydrogen peroxide (H2O2) to water (H2O), and the influence of SRB metabolites was much larger than that of bacterial cells...
September 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28904232/effects-of-high-versus-moderate-intensity-training-on-neuroplasticity-and-functional-recovery-after-focal-ischemia
#17
Caroline Pin-Barre, Annabelle Constans, Jeanick Brisswalter, Christophe Pellegrino, Jérôme Laurin
BACKGROUND AND PURPOSE: This study was designed to compare the effects of high-intensity interval training (HIT) and moderate-intensity aerobic training (MOD) on functional recovery and cerebral plasticity during the first 2 weeks after cerebral ischemia. METHODS: Rats were randomized as follows: control (n=15), SHAM (n=9), middle cerebral artery occlusion (n=13), middle cerebral artery occlusion at day 1 (n=7), MOD (n=13), and HIT (n=13). Incremental tests were performed at day 1 (D1) and 14 (D14) to identify the running speed associated with the lactate threshold (SLT) and the maximal speed (Smax)...
September 13, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28903007/characterization-of-repulsive-forces-and-surface-deformation-in-thin-micellar-films-via-afm
#18
Benjamin Locke Micklavzina, Marjorie L Longo
Here we examine how the force on an AFM tip varies as it approaches micellar surfactant films, and use this information to discern the film's surface structure and Young's Modulus. Rows of wormlike hemi-micelles were created at a graphite interface using 10 mM sodium dodecyl sulfate (SDS). We found that the repulsive force on a silicon nitride tip as it approached the surface was exponential, with a decay length of 2.0±0.1 nm. The addition of Na2SO4 was found to cause a change in this behavior, with a clear split into two exponential regions at concentrations above 1 mM...
September 13, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28902163/infants-and-children-s-salt-taste-perception-and-liking-a-review
#19
REVIEW
Djin G Liem
Sodium is an essential nutrient for the human body. It is widely used as sodium chloride (table salt) in (processed) foods and overconsumed by both children and adults, placing them at risk for adverse health effects such as high blood pressure and cardiovascular diseases. The current review focusses on the development of salt taste sensitivity and preferences, and its association with food intake. Three -to- four month old infants are able to detect and prefer sodium chloride solutions over plain water, which is thought to be a biological unlearned response...
September 13, 2017: Nutrients
https://www.readbyqxmd.com/read/28901673/catanionic-coacervate-micro-droplets-as-a-surfactant-based-membrane-free-protocell-model
#20
Jean-Paul Douliez, Nicolas Martin, Stephen Mann, Cédric Gaillard, Thomas Beneyton, Jean-Christophe Baret, Laure Beven
We report on the formation of surfactant complex catanionic coacervate micro-droplets in mixtures of decanoic acid and cetyl pyridinium chloride or cetyl trimethyl ammonium bromide. We show that coacervation occurs over a broad range of composition, pH and ionic strength. The catanionic coacervates consist of elongated micelles, sequester a wide range of solutes including water-soluble organic dyes, polysaccharides, proteins, enzymes and DNA, and can be structurally stabilized by sodium alginate or gelatin-based hydrogelation...
September 13, 2017: Angewandte Chemie
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