keyword
https://read.qxmd.com/read/38534387/all-but-small-mirnas-from-wharton-s-jelly-mesenchymal-stromal-cell-small-extracellular-vesicles-rescue-premature-white-matter-injury-after-intranasal-administration
#1
JOURNAL ARTICLE
Vera Tscherrig, Marel Steinfort, Valérie Haesler, Daniel Surbek, Andreina Schoeberlein, Marianne Simone Joerger-Messerli
White matter injury (WMI) is a common neurological issue in premature-born neonates, often causing long-term disabilities. We recently demonstrated a key beneficial role of Wharton's jelly mesenchymal stromal cell-derived small extracellular vesicles (WJ-MSC-sEVs) microRNAs (miRNAs) in WMI-related processes in vitro. Here, we studied the functions of WJ-MSC-sEV miRNAs in vivo using a preclinical rat model of premature WMI. Premature WMI was induced in rat pups through inflammation and hypoxia-ischemia. Small EVs were purified from the culture supernatant of human WJ-MSCs...
March 19, 2024: Cells
https://read.qxmd.com/read/38517158/excessive-fatty-acids-activate-prmt5-mdm2-drosha-pathway-to-regulate-mirna-biogenesis-and-lipid-metabolism
#2
JOURNAL ARTICLE
Aijun Hou, Xiaoding Xu, Yu Zhang, Hongxiu He, Yihan Feng, Wenhui Fan, Rongrong Tan, Likun Gong, Jing Chen
BACKGROUND: Excessive fatty acids in the liver lead to the accumulation of lipotoxic lipids and then cellular stress to further evoke the related disease, like non-alcoholic fatty liver disease (NAFLD). As reported, fatty acid stimulation can cause some specific miRNA dysregulation, which caused us to investigate the relationship between miRNA biogenesis and fatty acid overload. METHODS: Gene expression omnibus (GEO) dataset analysis, miRNA-seq, miRNA cleavage assay, RT-qPCR, western blotting, immunofluorescence and co-immunoprecipitation (co-IP) were used to reveal the change of miRNAs under pathological status and explore the relevant mechanism...
March 22, 2024: Liver International: Official Journal of the International Association for the Study of the Liver
https://read.qxmd.com/read/38509178/non-canonical-rna-substrates-of-drosha-lack-many-of-the-conserved-features-found-in-primary-microrna-stem-loops
#3
JOURNAL ARTICLE
Karen Gu, Lawrence Mok, Matthew J Wakefield, Mark M W Chong
The RNase III enzyme Drosha has a central role in microRNA (miRNA) biogenesis, where it is required to release the stem-loop intermediate from primary (pri)-miRNA transcripts. However, it can also cleave stem-loops embedded within messenger (m)RNAs. This destabilizes the mRNA causing target gene repression and appears to occur primarily in stem cells. While pri-miRNA stem-loops have been extensively studied, such non-canonical substrates of Drosha have yet to be characterized in detail. In this study, we employed high-throughput sequencing to capture all polyA-tailed RNAs that are cleaved by Drosha in mouse embryonic stem cells (ESCs) and compared the features of non-canonical versus miRNA stem-loop substrates...
March 20, 2024: Scientific Reports
https://read.qxmd.com/read/38472669/variant-analysis-of-mirna-regulatory-genes-in-35-sporadic-lung-carcinoma-tumors
#4
JOURNAL ARTICLE
Özkan Bağcı, Ebru Marzioğlu Özdemir, Batuhan Şanlıtürk
UNLABELLED: Lung cancer is one of the cancer types with the highest mortality worldwide. The most frequently mutated genes known to be clinically important in lung cancers are EGFR, BRAF, and KRAS genes. Therefore, the therapeutic agents developed are directed against variants that cause over-activation of the EGFR-KRAS-BRAF-BRAF-MEK/ERK signalling pathway. However, different responses of patients to Tyrosine Kinase Inhibitors (TKIs) suggest that new prognostic biomarkers should be defined and epigenetic mechanisms may be related to this situation...
March 12, 2024: Doklady. Biochemistry and Biophysics
https://read.qxmd.com/read/38365130/effect-of-heat-stress-on-the-hypothalamic-expression-of-water-channel-and-noncoding-rna-biogenesis-related-genes-in-modern-broilers-and-their-ancestor-red-jungle-fowl
#5
JOURNAL ARTICLE
Elizabeth S Greene, Travis W Tabler, Sara K Orlowski, Sami Dridi
Genetic selection for high growth rate has resulted in spectacular progress in feed efficiency in chickens. As feed intake and water consumption (WC) are associated and both are affected by environmental conditions, we evaluated WC and its hypothalamic regulation in three broiler-based research lines and their ancestor jungle fowl (JF) under heat stress (HS) conditions. Slow growing ACRB, moderate growing 95RB, fast growing MRB, and JF were exposed to daily chronic cyclic HS (36 °C, 9 h/d) or thermoneutral temperature (24 °C)...
February 14, 2024: Brain Research
https://read.qxmd.com/read/38343691/implications-of-snp-triggered-mirna-dysregulation-in-schizophrenia-development
#6
REVIEW
Fadumo Abdullahi Mohamed, Kristine Freude
This review examines the substantial involvement of Single Nucleotide Polymorphisms (SNPs) and microRNAs (miRNAs) in the etiology and susceptibility to Schizophrenia, with particular emphasis on the dopaminergic, glutamatergic, and GABAergic systems. It elucidates the potential of SNPs to disrupt miRNA-mRNA interactions, leading to alterations in the regulatory mechanisms of Schizophrenia risk genes and subsequently influencing the susceptibility to Schizophrenia. Specific attention is given to the impact of SNPs in DICER, DROSHA , and DGCR8 , as well as the potential for changes in DRD2 gene expression driven by miR-9 and miR-326, heightening the likelihood of Schizophrenia development...
2024: Frontiers in Genetics
https://read.qxmd.com/read/38241848/cytoplasmic-and-nuclear-drosha-in-human-villous-trophoblasts
#7
JOURNAL ARTICLE
Syunya Noguchi, Sadayuki Ohkura, Yasuyuki Negishi, Shohei Tozawa, Takami Takizawa, Rimpei Morita, Hironori Takahashi, Akihide Ohkuchi, Toshihiro Takizawa
In villous trophoblasts, DROSHA is a key ribonuclease III enzyme that processes pri-microRNAs (pri-miRNAs) into pre-miRNAs at the placenta-specific, chromosome 19 miRNA cluster (C19MC) locus. However, little is known of its other functions. We performed formaldehyde crosslinking, immunoprecipitation, and sequencing (fCLIP-seq) analysis of terminal chorionic villi to identify DROSHA-binding RNAs in villous trophoblasts. In villous trophoblasts, DROSHA predominantly generated placenta-specific C19MC pre-miRNAs, including antiviral C19MC pre-miRNAs...
January 2, 2024: Journal of Reproductive Immunology
https://read.qxmd.com/read/38226175/sars-cov-2-produces-a-microrna-cov2-mir-o8-in-patients-with-covid-19-infection
#8
JOURNAL ARTICLE
Elise J Tucker, Soon Wei Wong, Shashikanth Marri, Saira Ali, Anthony O Fedele, Michael Z Michael, Darling Rojas-Canales, Jordan Y Li, Chuan Kok Lim, Jonathan M Gleadle
Many viruses produce microRNAs (miRNAs), termed viral miRNAs (v-miRNAs), with the capacity to target host gene expression. Bioinformatic and cell culture studies suggest that SARS-CoV-2 can also generate v-miRNAs. This patient-based study defines the SARS-CoV-2 encoded small RNAs present in nasopharyngeal swabs of patients with COVID-19 infection using small RNA-seq. A specific conserved sequence (CoV2-miR-O8) is defined that is not expressed in other coronaviruses but is preserved in all SARS-CoV-2 variants...
January 19, 2024: IScience
https://read.qxmd.com/read/38200156/maintaining-drosha-expression-with-cdk5-inhibitors-as-a-potential-therapeutic-strategy-for-early-intervention-after-tbi
#9
JOURNAL ARTICLE
Lu Huang, Li Xia, Tiejian Nie, Bozhou Cui, Jianjun Lu, Fangfang Lu, Feiyan Fan, Dongni Ren, Yuan Lu, Guodong Gao, Qian Yang
Traumatic brain injury (TBI) is a major cause of death and disability in adults. The pathological process of TBI involves a multifactorial cascade in which kinases have been proven contribute to interactions between relevant factors and amplification of signaling cascades. Cyclin-dependent kinase 5 (Cdk5) is a promising kinase that has been implicated in various brain disorders, including TBI. However, the mechanism by which Cdk5 induces neuronal damage remains unclear. Here, we show for the first time that Drosha, a key enzyme in microRNA biogenesis, is a pivotal substrate of abnormally activated Cdk5...
January 10, 2024: Experimental & Molecular Medicine
https://read.qxmd.com/read/38109320/nuclear-localization-of-argonaute-2-is-affected-by-cell-density-and-may-relieve-repression-by-micrornas
#10
JOURNAL ARTICLE
Krystal C Johnson, Audrius Kilikevicius, Cristina Hofman, Jiaxin Hu, Yang Liu, Selina Aguilar, Jon Graswich, Yi Han, Tao Wang, Jill M Westcott, Rolf A Brekken, Lan Peng, Georgios Karagkounis, David R Corey
Argonaute protein is associated with post-transcriptional control of cytoplasmic gene expression through miRNA-induced silencing complexes (miRISC). Specific cellular and environmental conditions can trigger AGO protein to accumulate in the nucleus. Localization of AGO is central to understanding miRNA action, yet the consequences of AGO being in the nucleus are undefined. We show nuclear enrichment of AGO2 in HCT116 cells grown in two-dimensional culture to high density, HCT116 cells grown in three-dimensional tumor spheroid culture, and human colon tumors...
December 18, 2023: Nucleic Acids Research
https://read.qxmd.com/read/37993925/exosomal-mir-331-3p-derived-from-chemoresistant-osteosarcoma-cells-induces-chemoresistance-through-autophagy
#11
JOURNAL ARTICLE
Chenyang Meng, Yun Yang, Wei Feng, Penglei Ma, Rui Bai
BACKGROUND: Osteosarcoma is a common malignant bone tumor, and chemotherapy can effectively improve the prognosis. MicroRNA-331 (MiR-331) is associated with poor cancer outcomes. However, the role of miR-331 in osteosarcoma remains to be explored. METHODS: Drug-resistant osteosarcoma cells were cultured, and their exosomes were purified. The secretion and uptake of exosomes by drug-resistant osteosarcoma and osteosarcoma cells were confirmed using a fluorescence tracking assay and Transwell experiments...
November 22, 2023: Journal of Orthopaedic Surgery and Research
https://read.qxmd.com/read/37926315/role-of-mir-276-3p-in-the-cyantraniliprole-resistance-mechanism-of-bemisia-tabaci-via-cyp6cx3-targeting
#12
JOURNAL ARTICLE
Zanrong Wen, Kaixin Li, Wei Xu, Zhuang Zhang, Ni Liang, Moxian Chen, Lei Guo
The sweet potato whitefly, Bemisia tabaci, is an important insect pest that transmits over 200 different plant viruses and causes serious damage to the production of cotton and Solanaceae vegetables. Cyantraniliprole is the first diamide insecticide, showing toxicity against B. tabaci. However, B. tabaci has developed resistance to this insecticide by upregulating the expressions of cytochrome P450 genes such as CYP6CX3, while there is limited information on the regulatory mechanism mediated by miRNA. In the present study, ten miRNAs were predicted to target CYP6CX3, in which miR-276-3p showed an inverse expression pattern with CYP6CX3 in two cyantraniliprole resistant strains and under cyantraniliprole exposure...
November 3, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37925180/pri-mirna-cleavage-assays-for-the-microprocessor-complex
#13
JOURNAL ARTICLE
Thi Nhu-Y Le, Cong Truc Le, Tuan Anh Nguyen
The Microprocessor complex (MP) is a vital component in the biogenesis of microRNAs (miRNAs) in animals. It plays a crucial role in the biogenesis of microRNAs (miRNAs) in mammals as it cleaves primary miRNAs (pri-miRNAs) to initiate their production. The accurate enzymatic activity of MP is critical to ensuring proper sequencing and expression of miRNAs and their correct cellular functions. RNA elements in pri-miRNAs, including secondary structures and sequencing motifs, RNA editing and modifications, and cofactors, can impact MP cleavage and affect miRNA expression and sequence...
2023: Methods in Enzymology
https://read.qxmd.com/read/37890416/tert-promoter-mutations-and-gene-amplification-in-endometrial-cancer
#14
JOURNAL ARTICLE
Aaron M Praiss, Antonio Marra, Qin Zhou, Eric Rios-Doria, Amir Momeni-Boroujeni, Alexia Iasonos, Pier Selenica, David N Brown, Carol Aghajanian, Nadeem R Abu-Rustum, Lora H Ellenson, Britta Weigelt
OBJECTIVE: To assess the clinicopathologic, molecular profiles, and survival outcomes of patients with endometrial carcinomas (ECs) harboring telomerase reverse transcriptase (TERT) hotspot mutations or gene amplification. METHODS: ECs harboring somatic TERT promoter hotspot mutations or gene amplification (TERT-altered) were identified from 1944 ECs that underwent clinical tumor-normal sequencing from 08/2016-12/2021. Clinicopathologic variables, somatic mutation profiles, and survival outcomes of TERT-alt and TERT-wild-type EC were assessed...
December 2023: Gynecologic Oncology
https://read.qxmd.com/read/37889026/the-relationship-between-prognosis-of-patients-with-traumatic-brain-injury-and-microrna-biogenesis-proteins
#15
JOURNAL ARTICLE
Ayşe Çabukusta Acar, Şükran Burçak Yoldaş, Elif Sarıönder Gencer, İlker Öngüç Aycan, Suat Hayri Sanlı
BACKGROUND: This study aims to investigate whether the expression levels of proteins involved in microRNA (miRNA) biogenesis vary in early- and late-stage traumatic brain injury (TBI) patients and to evaluate its effect on prognosis. METHODS: Dicer, Drosha, DiGeorge Syndrome Critical Region eight (DGCR8), Exportin5 (XPO5), and Argonaute2 (AGO2) levels were measured in the blood samples of severe TBI patients collected 4-6 h and 72 h after the trauma and compared with the control group...
November 2023: Turkish Journal of Trauma & Emergency Surgery: TJTES
https://read.qxmd.com/read/37827159/dicer-ribonuclease-removes-harmful-r-loops
#16
JOURNAL ARTICLE
Lola P Camino, Arijit Dutta, Sonia Barroso, Carmen Pérez-Calero, Jeffrey N Katz, María García-Rubio, Patrick Sung, Belén Gómez-González, Andrés Aguilera
R-loops, which consist of a DNA-RNA hybrid and a displaced DNA strand, are known to threaten genome integrity. To counteract this, different mechanisms suppress R-loop accumulation by either preventing the hybridization of RNA with the DNA template (RNA biogenesis factors), unwinding the hybrid (DNA-RNA helicases), or degrading the RNA moiety of the R-loop (type H ribonucleases [RNases H]). Thus far, RNases H are the only nucleases known to cleave DNA-RNA hybrids. Now, we show that the RNase DICER also resolves R-loops...
October 19, 2023: Molecular Cell
https://read.qxmd.com/read/37823932/the-fluctuations-of-expression-profiles-of-critical-genes-in-the-mirna-maturation-process-and-pro-and-anti-inflammatory-cytokines-in-the-pathogenesis-and-progression-of-multiple-sclerosis
#17
JOURNAL ARTICLE
Fatemeh Khakdan, Athar Sadat Javanmard, Parisa Shahmoradipour, Maryam Jalali Jahromi
BACKGROUND: Multiple sclerosis (MS) is a central nervous system disease known for immune-mediated demyelination, inflammatory, and neurodegeneration symptoms. Discovering molecular biomarkers to classify RRMS and SPMS patients, monitor the disease activity, and response to particular treatments is one area that has received notable attraction. MicroRNA (miRNA), a single-stranded non-coding RNA molecule, is a significant regulator of gene expression recruited in pathogenic mechanisms in diverse diseases, especially cancer and MS...
October 12, 2023: Molecular Biology Reports
https://read.qxmd.com/read/37810246/essential-role-of-the-amino-terminal-region-of-drosha-for-the-microprocessor-function
#18
JOURNAL ARTICLE
Amit Prabhakar, Song Hu, Jin Tang, Prajakta Ghatpande, Giorgio Lagna, Xuan Jiang, Akiko Hata
Drosha is a core component of the Microprocessor complex that cleaves primary-microRNAs (pri-miRNAs) to generate precursor-miRNA and regulates the expression of ∼80 ribosomal protein (RP) genes. Despite the fact that mutations in the amino-terminal region of Drosha (Drosha-NTR) are associated with a vascular disorder, hereditary hemorrhagic telangiectasia, the precise function of Drosha-NTR remains unclear. By deleting exon 5 from the Drosha gene and generating a Drosha mutant lacking the NTR (ΔN), we demonstrate that ΔN is unable to process pri-miRNAs, which leads to a global miRNA depletion, except for the miR-183/96/182 cluster...
October 20, 2023: IScience
https://read.qxmd.com/read/37793530/review-non-canonical-role-of-drosha-ribonuclease-iii
#19
REVIEW
Xuanshuo Wei, Jin Tang, Chuwen Lin, Xuan Jiang
The typical function of Drosha is participating in cleaving pri-miRNA, the initial step of miRNA biogenesis, in the nucleus. Since Drosha has a double-stranded RNA-binding domain and two RNase III domains, when it binds and/or cleaves other RNA species other than pri-miRNA, Drosha is able to induce a variety of novel biological effects. Moreover, by interacting with other protein, Drosha is able to modify the function of other protein complexes. Recently, diverse non-classical functions of Drosha have been demonstrated, such as promoting DNA damage repair, transcriptional activation and inhibition, pre-mRNA splicing regulation, mRNA destabilization, and virus-host interaction...
October 2, 2023: International Journal of Biological Macromolecules
https://read.qxmd.com/read/37791873/drosha-dependent-micrornas-modulate-fus-mediated-neurodegeneration-in-vivo
#20
JOURNAL ARTICLE
Sukhleen Kour, Tyler Fortuna, Eric N Anderson, Darilang Mawrie, Jessica Bilstein, Ramakrishnan Sivasubramanian, Caroline Ward, Rishit Roy, Dhivyaa Rajasundaram, Jared Sterneckert, Udai Bhan Pandey
Mutations in the Fused in Sarcoma (FUS) gene cause the familial and progressive form of amyotrophic lateral sclerosis (ALS). FUS is a nuclear RNA-binding protein involved in RNA processing and the biogenesis of a specific set of microRNAs. Here we report that Drosha and two previously uncharacterized Drosha-dependent miRNAs are strong modulators of FUS expression and prevent the cytoplasmic segregation of insoluble mutant FUS in vivo. We demonstrate that depletion of Drosha mitigates FUS-mediated degeneration, survival and motor defects in Drosophila...
October 4, 2023: Nucleic Acids Research
keyword
keyword
47440
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.