N C Wortham, E Ahamed, S M Nicol, R S Thomas, M Periyasamy, J Jiang, A M Ochocka, S Shousha, L Huson, S E Bray, R C Coombes, S Ali, F V Fuller-Pace
The DEAD-box RNA helicases p68 (DDX5) and p72 (DDX17) have been shown to act as transcriptional co-activators for a diverse range of transcription factors, including oestrogen receptor-alpha (ERalpha). Here, we show that, although both proteins interact with and co-activate ERalpha in reporter gene assays, small interfering RNA-mediated knockdown of p72, but not p68, results in a significant inhibition of oestrogen-dependent transcription of endogenous ERalpha-responsive genes and oestrogen-dependent growth of MCF-7 and ZR75-1 breast cancer cells...
November 19, 2009: Oncogene