Tania Churasacari Vinces, Anacleto Silva de Souza, Cecília F Carvalho, Aline Biazola Visnardi, Raphael D Teixeira, Edgar E Llontop, Beatriz Aparecida Passos Bismara, Elisabete J Vicente, José O Pereira, Robson Francisco de Souza, Mauricio Yonamine, Sandro Roberto Marana, Chuck Shaker Farah, Cristiane R Guzzo
Herein, we present a novel esterase enzyme, Ade1, isolated from a metagenomic library of Amazonian dark earths soils, demonstrating its broad substrate promiscuity by hydrolyzing ester bonds linked to aliphatic groups. The three-dimensional structure of the enzyme was solved in the presence and absence of substrate (tributyrin), revealing its classification within the α/β-hydrolase superfamily. Despite being a monomeric enzyme, enzymatic assays reveal a cooperative behavior with a sigmoidal profile (initial velocities vs substrate concentrations)...
April 26, 2024: Biochemistry