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https://www.readbyqxmd.com/read/27910732/binding-of-anti-cardiovascular-drug-to-serum-albumin-an-insight-in-the-light-of-spectroscopic-and-computational-approaches
#1
Tajalli Ilm Chandel, Gulam Rabbani, MohsinVahid Khan, Masihuz Zaman, Parvez Alam, Yasser Shahein, Rizwan Hasan Khan
Isoprenaline hydrochloride is a potential cardiovascular drug helps in the smooth functioning of the heart muscles. So, we have performed the binding study of ISO with BSA. This study was investigated by UV absorption, fluorescence, synchronous fluorescence, circular dichroism etc. The analysis of intrinsic fluorescence data showed the low binding affinity of ISO. The binding constant Kb was 2.8×103 M-1 and binding stoichiometry (n) was approximately one and the Gibb's free energy change at 310 K was determined to be -8...
December 2, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27909797/wd40-repeat-protein-mocrec-is-essential-for-carbon-repression-and-is-involved-in-conidiation-growth-and-pathogenicity-of-magnaporthe-oryzae
#2
Khalid Abdelkarim Omer Matar, Xiaofeng Chen, Dongjie Chen, Wilfred Mabeche Anjago, Justice Norvienyeku, Yahong Lin, Meilian Chen, Zonghua Wang, Daniel J Ebbole, Guo-Dong Lu
Carbon catabolite repression (CCR) is a common regulatory mechanism used by microorganisms to prioritize use of a preferred carbon source (usually glucose). The CreC WD40-repeat protein is a major component of the CCR pathway in Aspergillus nidulans. To clarify the function of the CreC ortholog from Magnaporthe oryzae in regulating gene expression important for pathogenesis, MoCreC was identified and genetically characterized. The vegetative growth rate of the MoCreC deletion mutant on various carbon sources was reduced...
December 1, 2016: Current Genetics
https://www.readbyqxmd.com/read/27909291/engineering-of-a-thermostable-esterase-est816-to-improve-its-quorum-quenching-activity-and-the-underlying-structural-basis
#3
Xiwen Liu, Li-Chuang Cao, Xin-Jiong Fan, Yu-Huan Liu, Wei Xie
N-acyl-homoserine lactones (AHLs) are small diffusible molecules called autoinducers that mediate cell-to-cell communications. Enzymatic degradation of AHLs is a promising bio-control strategy known as quorum-quenching. To improve the quorum-quenching activity of a thermostable esterase Est816, which had been previously cloned, we have engineered the enzyme by random mutagenesis. One of the mutants M2 with double amino acid substitutions (A216V/K238N) showed 3-fold improvement on catalytic efficiency. Based on the crystal structure determined at 2...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27908721/stress-response-in-honeybees-is-associated-with-changes-in-task-related-physiology-and-energetic-metabolism
#4
Célia Bordier, Séverine Suchail, Maryline Pioz, Jean Marc Devaud, Claude Collet, Mercedes Charreton, Yves Le Conte, Cédric Alaux
In a rapidly changing environment, honeybee colonies are increasingly exposed to diverse sources of stress (e.g., new parasites, pesticides, climate warming), which represent a challenge to individual and social homeostasis. However, bee physiological responses to stress remain poorly understood. We therefore exposed bees specialised in different tasks (nurses, guards and foragers) to ancient (immune and heat stress) or historically more recent sources of stress (pesticides), and we determined changes in the expression of genes linked to behavioural maturation (vitellogenin - vg and juvenile hormone esterase - jhe) as well as in energetic metabolism (glycogen level, expression level of the receptor to the adipokinetic hormone - akhr, and endothermic performance)...
November 28, 2016: Journal of Insect Physiology
https://www.readbyqxmd.com/read/27907079/identification-of-pectin-degrading-enzymes-secreted-by-xanthomonas-oryzae-pv-oryzae-and-determination-of-their-role-in-virulence-on-rice
#5
Lavanya Tayi, Roshan V Maku, Hitendra Kumar Patel, Ramesh V Sonti
Xanthomonas oryzae pv.oryzae (Xoo) causes the serious bacterial blight disease of rice. Xoo secretes a repertoire of plant cell wall degrading enzymes (CWDEs) like cellulases, xylanases, esterases etc., which act on various components of the rice cell wall. The major cellulases and xylanases secreted by Xoo have been identified and their role in virulence has been determined. In this study, we have identified some of the pectin degrading enzymes of Xoo and assessed their role in virulence. Bioinformatics analysis indicated the presence of four pectin homogalacturonan (HG) degrading genes in the genome of Xoo...
2016: PloS One
https://www.readbyqxmd.com/read/27905486/biochemical-and-structural-analysis-of-a-novel-esterase-from-caulobacter-crescentus-related-to-penicillin-binding-protein-pbp
#6
Bum Han Ryu, Tri Duc Ngo, Wanki Yoo, Sojeong Lee, Boo-Young Kim, Euijoo Lee, Kyeong Kyu Kim, T Doohun Kim
Considering that the prevalence of antibiotic-resistant pathogenic bacteria is largely increasing, a thorough understanding of penicillin-binding proteins (PBPs) is of great importance and crucial significance because this enzyme family is a main target of β-lactam-based antibiotics. In this work, combining biochemical and structural analysis, we present new findings that provide novel insights into PBPs. Here, a novel PBP homologue (CcEstA) from Caulobacter crescentus CB15 was characterized using native-PAGE, mass spectrometry, gel filtration, CD spectroscopy, fluorescence, reaction kinetics, and enzyme assays toward various substrates including nitrocefin...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27905115/treatment-for-hereditary-angioedema-with-normal-c1-inh-and-specific-mutations-in-the-f12-gene-hae-fxii
#7
K Bork, K Wulff, G Witzke, J Hardt
Hereditary angioedema with normal C1 esterase inhibitor and mutations in the F12 gene (HAE-FXII) is associated with skin swellings, abdominal pain attacks, and the risk of asphyxiation due to upper airway obstruction. It occurs nearly exclusively in women. We report our experience treating HAE-FXII with discontinuation of potential trigger factors and drug therapies. The study included 72 patients with HAE-FXII. Potential triggers included estrogen-containing oral contraceptives (eOC), hormonal replacement therapy, or angiotensin-converting enzyme inhibitors...
November 6, 2016: Allergy
https://www.readbyqxmd.com/read/27904769/disruption-of-actin-filaments-and-suppression-of-pancreatic-cancer-cell-viability-and-migration-following-treatment-with-polyisoprenylated-cysteinyl-amides
#8
Augustine T Nkembo, Olufisayo Salako, Rosemary A Poku, Felix Amissah, Elizabeth Ntantie, Hernan Flores-Rozas, Nazarius S Lamango
Pancreatic cancer is characterized by K-Ras mutations in over 90% of the cases. The mutations make the tumors aggressive and resistant to current therapies resulting in very poor prognoses. Valiant efforts to drug mutant K-Ras and related proteins for the treatment of cancers with Ras mutations have been elusive. The need thus persists for therapies to target and suppress the hyperactive K-Ras mutant proteins to normal levels of activity. Polyisoprenylated cysteinyl amide inhibitors (PCAIs) of polyisoprenylated methylated protein methyl esterase (PMPMEase) were designed to disrupt polyisoprenylated protein metabolism and/or functions...
2016: American Journal of Cancer Research
https://www.readbyqxmd.com/read/27900690/a-microtiter-plate-based-assay-to-screen-for-active-and-stereoselective-hydrolytic-enzymes-in-enzyme-libraries
#9
Dominique Böttcher, Patrick Zägel, Marlen Schmidt, Uwe T Bornscheuer
A procedure for the high-throughput screening (HTS) of esterases is described. This includes a pretest for discrimination of active and inactive clones using an agar plate overlay assay, the enzyme expression in microtiter plates and the measurement of activity and enantioselectivity (E) of the esterase variants using acetates of secondary alcohols as model substrates. Acetic acid released is converted in an enzyme cascade leading to the stoichiometric formation of NADH, which is quantified in a spectrophotometer...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27899916/estdz3-a-new-esterolytic-enzyme-exhibiting-remarkable-thermostability
#10
Dimitra Zarafeta, Zalan Szabo, Danai Moschidi, Hien Phan, Evangelia D Chrysina, Xu Peng, Colin J Ingham, Fragiskos N Kolisis, Georgios Skretas
Lipolytic enzymes that retain high levels of catalytic activity when exposed to a variety of denaturing conditions are of high importance for a number of biotechnological applications. In this study, we aimed to identify new lipolytic enzymes, which are highly resistant to prolonged exposure to elevated temperatures. To achieve this, we searched for genes encoding for such proteins in the genomes of a microbial consortium residing in a hot spring located in China. After performing functional genomic screening on a bacterium of the genus Dictyoglomus, which was isolated from this hot spring following in situ enrichment, we identified a new esterolytic enzyme, termed EstDZ3...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27898037/analyses-of-evolutionary-characteristics-of-the-hemagglutinin-esterase-gene-of-influenza-c-virus-during-a-period-of-68-years-reveals-evolutionary-patterns-different-from-influenza-a-and-b-viruses
#11
Yuki Furuse, Yoko Matsuzaki, Hidekazu Nishimura, Hitoshi Oshitani
Infections with the influenza C virus causing respiratory symptoms are common, particularly among children. Since isolation and detection of the virus are rarely performed, compared with influenza A and B viruses, the small number of available sequences of the virus makes it difficult to analyze its evolutionary dynamics. Recently, we reported the full genome sequence of 102 strains of the virus. Here, we exploited the data to elucidate the evolutionary characteristics and phylodynamics of the virus compared with influenza A and B viruses...
November 26, 2016: Viruses
https://www.readbyqxmd.com/read/27896426/expression-and-characterization-of-a-codon-optimized-alkaline-stable-carbonic-anhydrase-from-aliivibrio-salmonicida-for-co2-sequestration-applications
#12
So-Young Jun, Sung Ho Kim, Bashista Kumar Kanth, Jinwon Lee, Seung Pil Pack
The CO2 mineralization process, accelerated by carbonic anhydrase (CA) was proposed for the efficient capture and storage of CO2, the accumulation of which in the atmosphere is the main cause of global warming. Here, we characterize a highly stable form of the cloned CA from the Gram-negative marine bacterium Aliivibrio salmonicida, named ASCA that can promote CO2 absorption in an alkaline solvent required for efficient carbon capture. We designed a mature form of ASCA (mASCA) using a codon optimization of ASCA gene and removal of ASCA signal peptide...
November 28, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27892561/aie-fluorophore-with-enhanced-cellular-uptake-for-tracking-esterase-activated-release-of-taurine-and-ros-scavenging
#13
Jun Wang, Yinglong Wu, Fang Zeng, Shuailing Huang, Shuizhu Wu
Fluorophores with aggregation-induced emission (AIE) characteristics are attractive and versatile tools for both chemical sensing and biological imaging. Herein, we designed and synthesized a fluorescent light-up system CTPE-Tau with enhanced cellular uptake ability. The system possesses several advantages, such as a large Stokes shift, low cytotoxicity, and good photostability. Also, it has been successfully utilized to track esterase-activated release of taurine and to scavenge intracellular ROS, and shows great potential for trackable visualized therapy...
November 28, 2016: Faraday Discussions
https://www.readbyqxmd.com/read/27891369/the-diagnostic-value-of-urinalysis-in-hemodialysis-patients-with-fever-sepsis-or-suspected-urinary-tract-infection
#14
Katerina G Oikonomou, Adib Alhaddad
INTRODUCTION: The diagnostic validity of urinalysis in asymptomatic Hemodialysis (HD) patients is low and there is limited data on the diagnostic value of urinalysis in HD patients with fever, sepsis, or suspected Urinary Tract Infection (UTI). AIM: The aim of this study was to assess the sensitivity, specificity, positive and negative predictive value of pyuria, bacteriuria, Leukocyte Esterase (LE) and nitrite positivity in symptomatic, febrile or/and septic HD patients...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27890013/in-silico-characterization-of-a-hypothetical-protein-rv1288-of-mycobacterium-tuberculosis-containing-an-esterase-signature-and-an-uncommon-lyte-domain
#15
Arbind Kumar, Pratibha Maan, Gurpreet Singh, Jagdeep Kaur
BACKGROUND: Death toll due to tuberculosis is still rising day by day. Whole genome sequence of Mycobacterium tuberculosis has provided a platform to conduct research in order to identify the probable drug target. OBJECTIVES: Out of 4000 gene products of M. tuberculosis, approximately 40% of proteins are annotated as hypothetical. Identifying and characterizing these proteins could provide a new prescriptive for developing new TB drugs. Rv1288, a protein of M. tuberculosis H37Rv has been annotated as a hypothetical protein in database...
November 24, 2016: Current Computer-aided Drug Design
https://www.readbyqxmd.com/read/27889517/rice-pectin-methylesterase-inhibitor28-ospmei28-encodes-a-functional-pmei-and-its-overexpression-results-in-a-dwarf-phenotype-through-increased-pectin-methylesterification-levels
#16
Hong Phuong Nguyen, Ho Young Jeong, Seung Ho Jeon, Donghyuk Kim, Chanhui Lee
Pectin methylesterases (PMEs, EC 3.1.1.11) belonging to carbohydrate esterase family 8 cleave the ester bond between a galacturonic acid and an methyl group and the resulting change in methylesterification level plays an important role during the growth and development of plants. Optimal pectin methylesterification status in each cell type is determined by the balance between PME activity and post-translational PME inhibition by PME inhibitors (PMEIs). Rice contains 49 PMEIs and none of them are functionally characterized...
November 18, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27886906/the-angiotensin-converting-enzyme-induced-angioedema
#17
REVIEW
Murat Bas
The bradykinin B2 receptor antagonist icatibant is effective in angiotensin-converting enzyme inhibitor-induced angioedema. The drug is not approved officially for this indication and has to be administered in an emergency situation off-label. Corticosteroids or antihistamines do not seem to work in this condition. The effectiveness of C1-esterase-inhibitor in angiotensin-converting enzyme-induced angioedema must be verified in a double-blind study.
February 2017: Immunology and Allergy Clinics of North America
https://www.readbyqxmd.com/read/27883027/disturbed-phospholipid-homeostasis-in-endoplasmic-reticulum-initiates-tri-o-cresyl-phosphate-induced-delayed-neurotoxicity
#18
Li Zhu, Pan Wang, Ying-Jian Sun, Ming-Yuan Xu, Yi-Jun Wu
Tri-o-cresyl phosphate (TOCP) is a widely used organophosphorus compound, which can cause a neurodegenerative disorder, i.e., organophosphate-induced delayed neurotoxicity (OPIDN). The biochemical events in the initiation of OPIDN were not fully understood except for the essential inhibition of neuropathy target esterase (NTE). NTE, located in endoplasmic reticulum (ER), catalyzes the deacylation of phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) to glycerophosphocholine (GPC). The present study aims to study the changes of ER phospholipids profile as well as levels of important intermediates of phospholipid synthesis such as diacylglycerol (DAG) and phosphatidic acid (PA) at the initiation stage of OPIDN...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27881148/susceptibility-profile-and-metabolic-mechanisms-involved-in-aedes-aegypti-and-aedes-albopictus-resistant-to-ddt-and-deltamethrin-in-the-central-african-republic
#19
Carine Ngoagouni, Basile Kamgang, Cécile Brengues, Gildas Yahouedo, Christophe Paupy, Emmanuel Nakouné, Mirdad Kazanji, Fabrice Chandre
BACKGROUND: Aedes aegypti and Ae. albopictus are the main epidemic vectors of dengue, chikungunya and Zika viruses worldwide. Their control during epidemics relies mainly on control of larvae and adults with insecticides. Unfortunately, loss of susceptibility of both species to several insecticide classes limits the efficacy of interventions. In Africa, where Aedes-borne viruses are of growing concern, few data are available on resistance to insecticides. To fill this gap, we assessed the susceptibility to insecticides of Ae...
November 24, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27878934/two-degradation-strategies-for-overcoming-the-recalcitrance-of-natural-lignocellulosic-xylan-by-polysaccharides-binding-gh10-and-gh11-xylanases-of-filamentous-fungi
#20
Youzhi Miao, Pan Li, Guangqi Li, Dongyang Liu, Irina S Druzhinina, Christian P Kubicek, Qirong Shen, Ruifu Zhang
The recalcitrance of lignocellulose forms a strong barrier for the bioconversion of lignocellulosic biomass in chemical or biofuel industries. Filamentous fungi are major plant biomass decomposer, and capable of forming all the required enzymes. Here, we characterized the GH10 and GH11 endo-xylanases and a CE1 acetyl-xylan esterase (Axe1) from a superior biomass-degrading strain, Aspergillus fumigatus Z5, and examined how they interact in xylan degradation. Cellulose-binding (CBM1) domain inhibited GH10 xylanase activities for pure xylan, but afforded them an ability to hydrolyze washed corncob particles (WCCP)...
November 23, 2016: Environmental Microbiology
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