Ya-Cheng Liao, Michael S Fernandopulle, Guozhen Wang, Heejun Choi, Ling Hao, Catherine M Drerup, Rajan Patel, Seema Qamar, Jonathon Nixon-Abell, Yi Shen, William Meadows, Michele Vendruscolo, Tuomas P J Knowles, Matthew Nelson, Magdalena A Czekalska, Greta Musteikyte, Mariam A Gachechiladze, Christina A Stephens, H Amalia Pasolli, Lucy R Forrest, Peter St George-Hyslop, Jennifer Lippincott-Schwartz, Michael E Ward
Long-distance RNA transport enables local protein synthesis at metabolically-active sites distant from the nucleus. This process ensures an appropriate spatial organization of proteins, vital to polarized cells such as neurons. Here, we present a mechanism for RNA transport in which RNA granules "hitchhike" on moving lysosomes. In vitro biophysical modeling, live-cell microscopy, and unbiased proximity labeling proteomics reveal that annexin A11 (ANXA11), an RNA granule-associated phosphoinositide-binding protein, acts as a molecular tether between RNA granules and lysosomes...
September 19, 2019: Cell