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https://www.readbyqxmd.com/read/28089962/mycoplasma-clearance-and-risk-analysis-in-a-model-bioprocess
#1
Julie Wang, Sarah Johnson, Matthew Brown, Scott Lute, Cyrus Agarabi, Alena Dabrazhynetskaya, Vladimir Chizhikov, Kurt Brorson
Mycoplasmas are a type of bacteria that lack cell walls and are occasional cell culture contaminants. In a biotechnology setting, because they can pass through 0.2 μm filters, mycoplasmas could pose a potential patient safety hazard if undetected contaminants from the production culture were not completely removed by downstream biotechnology manufacturing. In this study we investigated the ability of typical commercial monoclonal antibody (mAb) purification operations to clear and kill mycoplasmas, using Acholeplasma laidlawii as a model organism...
January 15, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28089693/effects-of-six-priority-controlled-phthalate-esters-with-long-term-low-dose-integrated-exposure-on-male-reproductive-toxicity-in-rats
#2
Hai-Tao Gao, Run Xu, Wei-Xin Cao, Liang-Liang Qian, Min Wang, Lingeng Lu, Qian Xu, Shu-Qin Yu
Human beings are inevitably exposed to ubiquitous phthalate esters (PEs) surroundings. The purposes of this study were to investigate the effects of long-term low-dose exposure to the mixture of six priority controlled phthalate esters (MIXPs): dimethyl phthalate (DMP), diethyl phthalate (DEP), di(n-butyl) phthalate (DBP), butyl benzyl phthalate (BBP), di(2-ethyhexyl) phthalate (DEHP) and di-n-octyl phthalate (DNOP), on male rat reproductive system and further to explore the underlying mechanisms of the reproductive toxicity...
January 12, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28042620/summarizing-simulation-results-using-causally-relevant-states
#3
Nidhi Parikh, Madhav Marathe, Samarth Swarup
As increasingly large-scale multiagent simulations are being implemented, new methods are becoming necessary to make sense of the results of these simulations. Even concisely summarizing the results of a given simulation run is a challenge. Here we pose this as the problem of simulation summarization: how to extract the causally-relevant descriptions of the trajectories of the agents in the simulation. We present a simple algorithm to compress agent trajectories through state space by identifying the state transitions which are relevant to determining the distribution of outcomes at the end of the simulation...
May 2016: Multiagent Based Simul
https://www.readbyqxmd.com/read/28033522/effects-of-nitrate-addition-on-water-column-methylmercury-in-occoquan-reservoir-virginia-usa
#4
Marc W Beutel, Ricardi Duvil, Francisco J Cubas, Thomas J Grizzard
Mercury bioaccumulation in aquatic biota poses a widespread threat to human and environmental health. Methylmercury (MeHg), the toxic form of mercury, tends to build up under anaerobic conditions in the profundal zones of lakes. In this study we performed a two-year assessment of spatial and temporal patterns of dissolved oxygen, nitrate, MeHg, manganese (Mn) and iron (Fe) in Occoquan Reservoir, a large run-of-the-river drinking water reservoir in Virginia, USA. A tributary to the reservoir receives input of nitrate-rich tertiary-treated wastewater that enhances the oxidant capacity of bottom water...
December 18, 2016: Water Research
https://www.readbyqxmd.com/read/27997328/pathological-jealousy-an-interactive-condition
#5
Mary V Seeman
OBJECTIVE: The aim of this review is to describe the psychopathology, antecedents, and current management of pathological jealousy from an interpersonal perspective. METHOD: The Google Scholar database was searched with the following terms: delusional jealousy; morbid jealousy; paranoid jealousy; pathological jealousy; Othello syndrome; delusional disorder-jealous type; conjugal paranoia. From a total of 600 articles, 40 were selected based on their currency and pertinence to the interpersonal aspects of jealousy...
2016: Psychiatry
https://www.readbyqxmd.com/read/27984678/assessing-the-ecosystem-level-consequences-of-a-small-scale-artisanal-kelp-fishery-within-the-context-of-climate-change
#6
K A Krumhansl, J N Bergman, A K Salomon
Coastal communities worldwide rely on small-scale artisanal fisheries as a means of increasing food security and alleviating poverty. Even small-scale fishing activities, however, are prone to resource depletion and environmental degradation, which can erode livelihoods in the long run. Thus, there is a pressing need to identify viable and resilient artisanal fisheries, and generate knowledge to support management within the context of a rapidly changing climate. We examined the ecosystem-level consequences of an artisanal kelp fishery (Macrocystis pyrifera), finding minimal impacts of small-scale harvest on kelp recovery rates, survival, and biomass dynamics, and abundances of associated commercial and culturally important fish species...
December 16, 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/27957780/an-uhplc-ms-ms-quantitative-method-for-trace-analysis-of-potential-endocrine-disrupting-steroid-hormones-in-estuarine-sediments
#7
Vanisree Mulabagal, Caleb Wilson, Joel S Hayworth
RATIONALE: Estuaries are dynamic ecosystems, providing vital habitat for unique organisms of great ecological and commercial importance. The influx of natural and synthetic steroid hormones into estuaries poses risks to these organisms and to broader ecosystem health. However, detecting these trace level pollutants in estuarine water and sediment requires improved analytical techniques. METHODS: We describe an optimized UHPLC-MS/MS method for simultaneous quantitation of four classes of steroid hormones (estrogens, glucocorticoids, androgens and progestins) in sediment samples collected from an Alabama estuary...
December 13, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27917249/perspectives-in-flow-based-microfluidic-gradient-generators-for-characterizing-bacterial-chemotaxis
#8
Christopher J Wolfram, Gary W Rubloff, Xiaolong Luo
Chemotaxis is a phenomenon which enables cells to sense concentrations of certain chemical species in their microenvironment and move towards chemically favorable regions. Recent advances in microbiology have engineered the chemotactic properties of bacteria to perform novel functions, but traditional methods of characterizing chemotaxis do not fully capture the associated cell motion, making it difficult to infer mechanisms that link the motion to the microbiology which induces it. Microfluidics offers a potential solution in the form of gradient generators...
November 2016: Biomicrofluidics
https://www.readbyqxmd.com/read/27915142/atrazine-triggers-hepatic-oxidative-stress-and-apoptosis-in-quails-coturnix-c-coturnix-via-blocking-nrf2-mediated-defense-response
#9
Cong Zhang, Xue-Nan Li, Li-Run Xiang, Lei Qin, Jia Lin, Jin-Long Li
The bioaccumulation and environmental persistence of atrazine (ATZ) poses a severe hazard to animal ecosystem. Quail has strong sensitivity to environmental pollutant, thus it is one of the most important ecological pollution indicator. However, true proof for the effects of ATZ exposure on the liver of quails is lacking. To evaluate the liver injury and the role of Nrf2-mediated defense responses during ATZ exposure, male quails were treated with ATZ (0, 50, 250 and 500mg/kg) by oral gavage for 45 days. Histopathological and ultrastructural changes, oxidative stress indices, apoptosis-related factors and Nrf2 pathway were detected...
March 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27906367/cultivation-of-lemon-fields
#10
Sushanta Kumar Pal, P Senthilkumaran
The sign rule requires that adjacent singularities on a contour have opposite signs and hence cultivation of lemon only fields poses problem as all lemons have positive index. In this paper we show that the interference of three linearly polarized plane waves can create regions of ellipse and vector fields in 2-dimensions (2D) in which a lemon lattice is interlaced in a V-point lattice. The lemons appear at intensity maxima of the lattice structure while the V-points take care of index conservation by sitting at intensity minima...
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27886526/food-and-beverage-product-reformulation-as-a-corporate-political-strategy
#11
C Scott, B Hawkins, C Knai
Product reformulation- the process of altering a food or beverage product's recipe or composition to improve the product's health profile - is a prominent response to the obesity and noncommunicable disease epidemics in the U.S. To date, reformulation in the U.S. has been largely voluntary and initiated by actors within the food and beverage industry. Similar voluntary efforts by the tobacco and alcohol industry have been considered to be a mechanism of corporate political strategy to shape public health policies and decisions to suit commercial needs...
January 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/27863713/improving-the-productivity-of-a-multidimensional-chromatographic-preparative-system-by-collecting-pure-chemicals-after-each-of-three-chromatographic-dimensions
#12
Danilo Sciarrone, Sebastiano Pantò, Paola Donato, Luigi Mondello
The enhanced sample collection capability of a heart-cutting three-dimensional GC-prep system is reported. In its original configuration, a highly pure component can be usually collected after the last (3D) column outlet by means of a dedicated preparative station. The latter is located after the last chromatographic column, and this poses the requirement for multiple heart cuts even for those components showing satisfactory degree of purity after the first (or second) separation dimension. The feasibility to collect pure components after each chromatographic dimension is here described, employing a three-dimension MDGC system equipped with high-temperature valves, located inside the first and second GC ovens, with the aim to improve the productivity of the collection procedure...
December 2, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27837116/predicting-hairline-fractures-in-eggs-of-mature-hens
#13
S van Mourik, B P G J Alders, F Helderman, L J F van de Ven, P W G Groot Koerkamp
Eggshell damage poses a serious problem for the consumption egg industry. Increasing the maximum age of laying hens will increase eggshell damage due to loss of shell strength. This poses a serious problem for automatic collection, packing, and transport. We performed a model based study focused on hairline fractures in eggs of 88-week-old hens, and simulated side collisions on 1,235 eggs using a specially designed pendulum. The kinetic energy at the moment of impact was related to the accelerations measured by an electronic egg going through the transport chain...
November 11, 2016: Poultry Science
https://www.readbyqxmd.com/read/27779324/fate-and-risk-of-atrazine-and-sulfentrazone-to-nontarget-species-at-an-agriculture-site
#14
Jordan L Thorngren, Amanda D Harwood, Tracye M Murphy, Kara E Huff Hartz, Courtney Y Fung, Michael J Lydy
The present study evaluated the risk associated with the application and co-occurrence of 2 herbicides, atrazine and sulfentrazone, applied to a 32-ha corn and soybean rotational field. Field concentrations of the compounds were measured in soil, runoff water, and groundwater, with peak mean atrazine and sulfentrazone concentrations found in the soil (144 ng/g dry wt, and 318 ng/g dry wt, respectively). Individual and mixture laboratory bioassays were conducted to determine the effects of atrazine and sulfentrazone on the survival of Daphnia magna and Pimephales promelas, the germination of Lactuca sativa, and the growth of Pseudokirchneriella subcapita and Lemna minor...
October 25, 2016: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/27747741/the-relationship-between-age-and-driving-attitudes-and-behaviors-among-older-americans
#15
Alexander J Mizenko, Brian C Tefft, Lindsay S Arnold, Jurek G Grabowski
BACKGROUND: Due to a decreasing birth rate and longer life expectancy, the proportion of Americans over the age of 65 is expected to rise in coming years. Drivers over 65 drive two billion miles yearly, a number that will increase. For that reason, it is imperative to understand their attitudes and perceptions. It is also important to understand whether drivers over 65 can be treated as one cohesive group, or if there are differences among them. METHODS: A web-enabled survey was conducted among Americans in the years 2011-2013...
December 2015: Injury Epidemiology
https://www.readbyqxmd.com/read/27743349/computational-tracking-of-shear-mediated-platelet-interactions-with-von-willebrand-factor
#16
Adam Ralph, Martin Somers, Jonathan Cowman, Bruno Voisin, Emma Hogan, Hannah Dunne, Eimear Dunne, Barry Byrne, Nigel Kent, Antonio J Ricco, Dermot Kenny, Simon Wong
The imaging of shear-mediated dynamic platelet behavior interacting with surface-immobilized von Willebrand factor (vWF) has tremendous potential in characterizing changes in platelet function for clinical diagnostics purposes. However, the imaging output, a series of images representing platelets adhering and rolling on the surface, poses unique, non-trivial challenges for software algorithms that reconstruct the positional trajectories of platelets. We report on an algorithm that tracks platelets using the output of such flow run experiments, taking into account common artifacts encountered by previously-published methods, and we derive seven key metrics of platelet dynamics that can be used to characterize platelet function...
December 2016: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/27720255/low-drift-nozzles-vs-standard-nozzles-for-pesticide-application-in-the-biological-efficacy-trials-of-pesticides-in-apple-pest-and-disease-control
#17
Grzegorz Doruchowski, Waldemar Świechowski, Sylwester Masny, Alicja Maciesiak, Małgorzata Tartanus, Hanna Bryk, Ryszard Hołownicki
The coarse spray air-induction nozzles have documented pesticide drift reducing potential and hence pose lower risk of environmental pollution than the standard fine spray hollow cone nozzles. However, it is questioned that use of the low-drift nozzles might not provide as effective crop protection as the standard nozzles. The objective of work was to assess the pest and disease control efficacy as affected by spray volume rate and nozzle type. The experiment was carried out in apple orchard, cv Jonagold/M26...
January 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/27703948/recurrent-patellar-instabilty-culminating-in-a-vertically-rotated-and-a-locked-patellar-dislocation-a-rare-entity
#18
Devgan A, Rohilla R, Jain A, Mehta H, Singh S
INTRODUCTION: Locked vertical patellar dislocations are rare and pose a therapeutic challenge. This case is more unusual, as the patient was a known case of recurrent patellar dislocation and presented with an atraumatic locked and vertically rotated patellar dislocation. This type of presentation has never been reported in literature to the best of our knowledge. CASE PRESENTATION: A 14-year-old healthy male child with previous history of recurrent lateral dislocation of patella presented to accident & emergency department with complaints of inability to walk or bear weight on his left lower limb after he spontaneously dislocated his patella while running on uneven ground...
April 2016: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/27649310/a-fence-that-eats-the-weed-alginate-lyase-immobilization-on-ultrafiltration-membrane-for-fouling-mitigation-and-flux-recovery
#19
Pradnya Meshram, Rachna Dave, Hiren Joshi, Gopal Dharani, Ramalingam Kirubagaran, Vayalam P Venugopalan
Polysaccharide fouling poses a significant challenge in the widespread application of membrane filtration for water purification. In order to mitigate the problem, a polysaccharide-degrading enzyme alginate lyase (Alg L; EC 4.2.2.3) was successfully immobilized on cellulose acetate ultrafiltration membrane using a dead-end filtration unit. Attenuated total reflectance Fourier transform infrared microscopy confirmed covalent linkage of the Alg L to the membrane. HPLC and Alg L activity studies confirmed that Alg L in immobilized form was enzymatically active...
December 2016: Chemosphere
https://www.readbyqxmd.com/read/27602589/identification-of-dual-ligands-targeting-angiotensin-ii-type-1-receptor-and-peroxisome-proliferator-activated-receptor-%C3%AE-by-core-hopping-of-telmisartan
#20
Jun Zhang, Xin Liu, Shu-Qing Wang, Gui-You Liu, Wei-Ren Xu, Xian-Chao Cheng, Run-Ling Wang
It has been reported previously that some angiotensin II receptor blockers not only antagonize angiotensin II type 1 receptor (AT1R), but also exert stimulation in peroxisome proliferator-activated receptor γ (PPARγ) partial activation, among which telmisartan displays the best. Telmisartan has been tested as a bifunctional ligand with antihypertensive and hypoglycemic activity. Aiming at more potent leads with selective AT1R antagonism and PPARγ partial agonism, the three parts of telmisartan including the distal benzimidazole ring, the biphenyl moiety, and the carboxylic acid group experienced modification by core hopping method in our study...
September 7, 2016: Journal of Biomolecular Structure & Dynamics
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