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Chenlin Pei, Chao Zhao, An-Jiang Wang, Anya X Fan, Viktoriya Grinchuk, Allen Smith, Rex Sun, Yue Xie, Nonghua Lu, Joseph F Urban, Terez Shea-Donohue, Aiping Zhao, Zhonghan Yang
Infection of humans with parasitic nematodes especially gastrointestinal geohelminths affect hundreds of millions of people worldwide and thus poses a major risk to global health. The host defense mechanism against enteric nematode infection remains to be fully understood, but it involves a polarized type 2 immunity leading to alterations in intestinal function that facilitates worm expulsion. We investigated the role of IL-25 in host protection against Heligmosomoides (H.) polygyrus bakeri infection in mice...
September 12, 2016: Infection and Immunity
Jee-Boong Lee, Chun-Yu Chen, Bo Liu, Luke Mugge, Pornpimon Angkasekwinai, Valeria Facchinetti, Chen Dong, Yong-Jun Liu, Marc E Rothenberg, Simon P Hogan, Fred D Finkelman, Yui-Hsi Wang
BACKGROUND: Food-mediated allergic reactions have emerged as a major health problem. The underlying mechanisms that promote uncontrolled type 2 immune responses to dietary allergens in the gastrointestinal tract remain elusive. OBJECTIVE: We investigated whether altering IL-25 signaling enhances or attenuates allergic responses to food allergens. METHODS: Mice of an IL-25 transgenic mouse line (iIL-25Tg mice), which constitutively overexpress intestinal IL-25, and Il17rb(-/-) mice, in which Il17rb gene expression is disrupted, were sensitized and gavage fed with ovalbumin (OVA)...
April 2016: Journal of Allergy and Clinical Immunology
Priya Rajavelu, Gang Chen, Yan Xu, Joseph A Kitzmiller, Thomas R Korfhagen, Jeffrey A Whitsett
Epithelial cells that line the conducting airways provide the initial barrier and innate immune responses to the abundant particles, microbes, and allergens that are inhaled throughout life. The transcription factors SPDEF and FOXA3 are both selectively expressed in epithelial cells lining the conducting airways, where they regulate goblet cell differentiation and mucus production. Moreover, these transcription factors are upregulated in chronic lung disorders, including asthma. Here, we show that expression of SPDEF or FOXA3 in airway epithelial cells in neonatal mice caused goblet cell differentiation, spontaneous eosinophilic inflammation, and airway hyperresponsiveness to methacholine...
May 2015: Journal of Clinical Investigation
Bertram Bleck, Angeliki Kazeros, Keren Bakal, Lymaris Garcia-Medina, Alexandra Adams, Mengling Liu, Richard A Lee, Doris B Tse, Amanda Chiu, Gabriele Grunig, John P Egan, Joan Reibman
BACKGROUND: Noninvasive sputum sampling has enabled the identification of biomarkers in asthmatic patients. Studies of discrete cell populations in sputum can enhance measurements compared with whole sputum in which changes in rare cells and cell-cell interactions can be masked. OBJECTIVE: We sought to enrich for sputum-derived human bronchial epithelial cells (sHBECs) and sputum-derived myeloid type 1 dendritic cells (sDCs) to describe transcriptional coexpression of targets associated with a type 2 immune response...
September 2015: Journal of Allergy and Clinical Immunology
Paola Tortorella, Maria Marcella Laganà, Marina Saresella, Eleonora Tavazzi, Maria Giulia Preti, Cristian Ricci, Francesca Baglio, Ivana Marventano, Federica Piancone, Giuseppe Baselli, Pietro Cecconi, Domenico Caputo, Mario Clerici, Marco Rovaris
Multiple sclerosis (MS) is characterized by a wide interpatient clinical variability and available biomarkers of disease severity still have suboptimal reliability. We aimed to assess immunological and MRI-derived measures of brain tissue damage in patients with different motor impairment degrees, for in vivo investigating the pathogenesis of MS-related disability. Twenty-two benign (B), 26 secondary progressive (SP), and 11 early, nondisabled relapsing-remitting (RR) MS patients and 37 healthy controls (HC) underwent conventional and diffusion tensor brain MRI and, as regards MS patients, immunophenotypic and functional analysis of stimulated peripheral blood mononuclear cells (PBMC)...
2014: BioMed Research International
L Tordesillas, C Gómez-Casado, M Garrido-Arandia, A Murua-García, A Palacín, J Varela, P Konieczna, J Cuesta-Herranz, C A Akdis, L O'Mahony, A Díaz-Perales
BACKGROUND: Since intestinal absorption of food protein can trigger an allergic reaction, the effect of plant food allergen on intestinal epithelial cell permeability and its ability to cross the epithelial monolayer was evaluated. OBJECTIVE: To study the interaction of Pru p 3 with intestinal epithelium, its natural entrance, analyzing transport kinetics and cellular responses that trigger. METHODS: This was achieved using Pru p 3, the peach LTP, as a model...
December 2013: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
X Yu, R Pappu, V Ramirez-Carrozzi, N Ota, P Caplazi, J Zhang, D Yan, M Xu, W P Lee, J L Grogan
Immune responses at mucosal barriers are regulated by innate type 2 lymphoid cells (ILC2s) that elaborate effector cytokines interleukins 5 and 13 (IL5 and IL13). IL25 and IL33 are key cytokines that support ILC2s; however, mice deficient in these pathways retain some functional ILC2s. Analysis of human and murine cells revealed that ILC2s highly express tumor necrosis factor (TNF)-receptor superfamily member DR3 (TNFRSF25). Engagement of DR3 with cognate ligand TL1A promoted ILC2 expansion, survival, and function...
May 2014: Mucosal Immunology
Wei Lv, Qianglin Duan, Lemin Wang, Zhu Gong, Fan Yang, Yanli Song
The aim of this study was to investigate the differential gene expression of cytokines in peripheral blood mononuclear cells (PBMCs) from patients with pulmonary embolism (PE) and controls. Twenty patients with PE and twenty control patients matched for gender and age with the PE group were recruited into the study. Human cDNA microarray analysis was used to detect differences in the expression of cytokine-associated genes between the two groups. In PE patients, the expression levels of the genes encoding IFNα5, IFNα6, IFNα8, IFNα14, IFNκ, IFNω1, IFNε1 and IFNγ were significantly lower compared with controls (P<0...
April 2013: Molecular Medicine Reports
Maira Gironi, Marina Saresella, Marco Rovaris, Matilde Vaghi, Raffaello Nemni, Mario Clerici, Enzo Grossi
UNLABELLED: HASH(0x47de938) BACKGROUND: Multiple Sclerosis (MS) is a multi-factorial disease, where a single biomarker unlikely can provide comprehensive information. Moreover, due to the non-linearity of biomarkers, traditional statistic is both unsuitable and underpowered to dissect their relationship. Patients affected with primary (PP=14), secondary (SP=33), benign (BB=26), relapsing-remitting (RR=30) MS, and 42 sex and age matched healthy controls were studied...
2013: Immunity & Ageing: I & A
Jianing Li, Yu Wang, Lihua Tang, Willem J S de Villiers, Donald Cohen, Jerold Woodward, Fred D Finkelman, Erik R M Eckhardt
BACKGROUND: The prevalence of peanut allergies is increasing. Peanuts and many other allergen sources contain significant amounts of triglycerides, which affect absorption of antigens but have unknown effects on sensitization and anaphylaxis. We recently reported that dietary medium-chain triglycerides (MCTs), which bypass mesenteric lymph and directly enter portal blood, reduce intestinal antigen absorption into blood compared with long-chain triglycerides (LCTs), which stimulate mesenteric lymph flow and are absorbed in chylomicrons through mesenteric lymph...
February 2013: Journal of Allergy and Clinical Immunology
Qianglin Duan, Wei Lv, Lemin Wang, Zhu Gong, Qiang Wang, Haoming Song, Hao Wang
Few studies have investigated the changes of Th1- and Th2-type cytokines in pulmonary embolism (PE) patients. In this study, the gene expression of interleukins and the balance of Th1- and Th2-type cytokines in the peripheral blood mononuclear cells (PBMCs) of PE patients and controls were investigated. A total of 20 PE patients and 20 gender- and age-matched controls were included in the study. Human cDNA microarray analysis was used to detect the differences in cytokine gene expression between the two groups and a random variance model corrected t-test was used to analyze the statistical data...
January 2013: Molecular Medicine Reports
M R Green, E Camilleri, M K Gandhi, J Peake, L R Griffiths
Many primary immunodeficiency disorders of differing etiologies have been well characterized, and much understanding of immunological processes has been gained by investigating the mechanisms of disease. Here, we have used a whole-genome approach, employing single-nucleotide polymorphism and gene expression microarrays, to provide insight into the molecular etiology of a novel immunodeficiency disorder. Using DNA copy number profiling, we define a hyperploid region on 14q11.2 in the immunodeficiency case associated with the interleukin (IL)-25 locus...
December 2011: Genes and Immunity
Anton Gossner, Anna Peers, Virginia Venturina, John Hopkins
This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M(r) 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M(r) 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical...
February 15, 2011: Veterinary Immunology and Immunopathology
Steven A Saenz, Mark C Siracusa, Jacqueline G Perrigoue, Sean P Spencer, Joseph F Urban, Joel E Tocker, Alison L Budelsky, Melanie A Kleinschek, Robert A Kastelein, Taku Kambayashi, Avinash Bhandoola, David Artis
CD4(+) T helper 2 (T(H)2) cells secrete interleukin (IL)4, IL5 and IL13, and are required for immunity to gastrointestinal helminth infections. However, T(H)2 cells also promote chronic inflammation associated with asthma and allergic disorders. The non-haematopoietic-cell-derived cytokines thymic stromal lymphopoietin, IL33 and IL25 (also known as IL17E) have been implicated in inducing T(H)2 cell-dependent inflammation at mucosal sites, but how these cytokines influence innate immune responses remains poorly defined...
April 29, 2010: Nature
Daniel R Neill, See Heng Wong, Agustin Bellosi, Robin J Flynn, Maria Daly, Theresa K A Langford, Christine Bucks, Colleen M Kane, Padraic G Fallon, Richard Pannell, Helen E Jolin, Andrew N J McKenzie
Innate immunity provides the first line of defence against invading pathogens and provides important cues for the development of adaptive immunity. Type-2 immunity-responsible for protective immune responses to helminth parasites and the underlying cause of the pathogenesis of allergic asthma-consists of responses dominated by the cardinal type-2 cytokines interleukin (IL)4, IL5 and IL13 (ref. 5). T cells are an important source of these cytokines in adaptive immune responses, but the innate cell sources remain to be comprehensively determined...
April 29, 2010: Nature
Yue Ma, Lydia Visser, Han Roelofsen, Marcel de Vries, Arjan Diepstra, Gustaaf van Imhoff, Tineke van der Wal, Marjan Luinge, Gloria Alvarez-Llamas, Hans Vos, Sibrand Poppema, Roel Vonk, Anke van den Berg
Hodgkin and Reed-Sternberg (HRS) cells in Hodgkin lymphoma (HL) secrete factors that interact with inflammatory background cells and may serve as biomarkers for disease activity. To detect new proteins related to pathogenesis, we analyzed the secretome of HRS cells. Proteins in cell culture supernatant of 4 HL cell lines were identified using 1DGE followed by in-gel trypsin digestion and LC-MS/MS. In total, 1290 proteins, including 368 secreted proteins, were identified. Functional grouping of secreted proteins revealed 37 proteins involved in immune response...
February 15, 2008: Blood
Melanie A Kleinschek, Alexander M Owyang, Barbara Joyce-Shaikh, Claire L Langrish, Yi Chen, Daniel M Gorman, Wendy M Blumenschein, Terrill McClanahan, Frank Brombacher, Stephen D Hurst, Robert A Kastelein, Daniel J Cua
Interleukin (IL)-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. We now show that IL-25 also regulates the development of autoimmune inflammation mediated by IL-17-producing T cells. We have generated IL-25-deficient (il25-/-) mice and found that they are highly susceptible to experimental autoimmune encephalomyelitis (EAE). The accelerated disease in the il25-/- mice is associated with an increase of IL-23 in the periphery and a subsequent increase in the number of inflammatory IL-17-, IFNgamma-, and TNF-producing T cells that invade the central nervous system...
January 22, 2007: Journal of Experimental Medicine
Padraic G Fallon, Sarah J Ballantyne, Niamh E Mangan, Jillian L Barlow, Ayan Dasvarma, Duncan R Hewett, Ann McIlgorm, Helen E Jolin, Andrew N J McKenzie
Type 2 immunity, which involves coordinated regulation of innate and adaptive immune responses, can protect against helminth parasite infection, but may lead to allergy and asthma after inappropriate activation. We demonstrate that il25(-/-) mice display inefficient Nippostrongylus brasiliensis expulsion and delayed cytokine production by T helper 2 cells. We further establish a key role for interleukin (IL)-25 in regulating a novel population of IL-4-, IL-5-, IL-13-producing non-B/non-T (NBNT), c-kit+, FcepsilonR1- cells during helminth infection...
April 17, 2006: Journal of Experimental Medicine
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