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https://www.readbyqxmd.com/read/29331515/mhc-class-i-loaded-ligands-from-breast-cancer-cell-lines-a-potential-hla-i-typed-antigen-collection
#1
Dmitri V Rozanov, Nikita D Rozanov, Kami Chiotti, Ashok Reddy, Phillip A Wilmarth, Larry L David, Seung W Cha, Sunghee Woo, Pavel Pevzner, Vineet Bafna, Gregory G Burrows, Juha K Rantala, Trevor Levin, Pavana Anur, Katie Johnson-Camacho, Shaadi Tabatabaei, Daniel J Munson, Tullia C Bruno, Jill E Slansky, John W Kappler, Naoto Hirano, Sebastian Boegel, Bernard A Fox, Colt Egelston, Diana L Simons, Grecia Jimenez, Peter P Lee, Joe W Gray, Paul T Spellman
To build a catalog of peptides presented by breast cancer cells, we undertook systematic MHC class I immunoprecipitation followed by elution of MHC class I-loaded peptides in breast cancer cells. We determined the sequence of 3196 MHC class I ligands representing 1921 proteins from a panel of 20 breast cancer cell lines. After removing duplicate peptides, i.e., the same peptide eluted from more than one cell line, the total number of unique peptides was 2740. Of the unique peptides eluted, more than 1750 had been previously identified, and of these, sixteen have been shown to be immunogenic...
January 10, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29330541/19f-perfluorocarbon-labeled-human-peripheral-blood-mononuclear-cells-can-be-detected-in-vivo-using-clinical-mri-parameters-in-a-therapeutic-cell-setting
#2
Corby Fink, Jeffrey M Gaudet, Matthew S Fox, Shashank Bhatt, Sowmya Viswanathan, Michael Smith, Joseph Chin, Paula J Foster, Gregory A Dekaban
A 19Fluorine (19F) perfluorocarbon cell labeling agent, when employed with an appropriate cellular MRI protocol, allows for in vivo cell tracking. 19F cellular MRI can be used to non-invasively assess the location and persistence of cell-based cancer vaccines and other cell-based therapies. This study was designed to determine the feasibility of labeling and tracking peripheral blood mononuclear cells (PBMC), a heterogeneous cell population. Under GMP-compliant conditions human PBMC were labeled with a 19F-based MRI cell-labeling agent in a manner safe for autologous re-injection...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330173/seasonal-variations-in-tuberculosis-diagnosis-among-hiv-positive-individuals-in-southern-africa-analysis-of-cohort-studies-at-antiretroviral-treatment-programmes
#3
Marie Ballif, Kathrin Zürcher, Stewart E Reid, Andrew Boulle, Matthew P Fox, Hans W Prozesky, Cleophas Chimbetete, Marcel Zwahlen, Matthias Egger, Lukas Fenner
OBJECTIVES: Seasonal variations in tuberculosis diagnoses have been attributed to seasonal climatic changes and indoor crowding during colder winter months. We investigated trends in pulmonary tuberculosis (PTB) diagnosis at antiretroviral therapy (ART) programmes in Southern Africa. SETTING: Five ART programmes participating in the International Epidemiology Database to Evaluate AIDS in South Africa, Zambia and Zimbabwe. PARTICIPANTS: We analysed data of 331 634 HIV-positive adults (>15 years), who initiated ART between January 2004 and December 2014...
January 11, 2018: BMJ Open
https://www.readbyqxmd.com/read/29330092/a-novel-double-antigen-sandwich-elisa-for-the-species-independent-detection-of-crimean-congo-hemorrhagic-fever-virus-specific-antibodies
#4
Miriam A Sas, Loic Comtet, Fabien Donnet, Marc Mertens, Zati Vatansever, Noel Tordo, Philippe Pourquier, Martin H Groschup
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease in humans caused by the CCHF virus (CCHFV). The detection of anti-CCHFV antibodies in animals is used to reveal infection risk areas. Therefore a simple, quick and reliable multispecies assay for the detection of CCHFV-specific antibodies is needed. This work presents the development and validation of a novel CCHF double-antigen ELISA for the detection of anti-CCHFV nucleoprotein antibodies. The test requires 30 μl of serum, and results are obtained within 90 min...
January 9, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29329889/exploring-individual-adaptations-to-an-anterior-cruciate-ligament-injury-prevention-programme
#5
Aaron S Fox, Jason Bonacci, Scott G McLean, Natalie Saunders
BACKGROUND: Individual responses to anterior cruciate ligament injury prevention programmes (ACL IPPs) have received little attention. This study examined the effects of an ACL IPP on neuromuscular control and lower limb biomechanics during landing at the group and individual levels. METHODS: Sixteen female athletes were randomly allocated to training (n=8) or control (n=8) groups. Electromyography, and three-dimensional kinematic and kinetic data were collected during landing at two testing sessions...
January 9, 2018: Knee
https://www.readbyqxmd.com/read/29329255/peri-implant-behavior-of-sloped-shoulder-dental-implants-used-for-all-on-four-protocols-an-histomorphometric-analysis-in-dogs
#6
Jose Luis Calvo Guirado, Aldo Fabian Lucero-Sánchez, Ana Boquete Castro, Marcus Abboud, Sergio Gehrke, Manuel Fernández Dominguez, Rafael Arcesio Delgado Ruiz
The aim of this study was to evaluate the soft tissue thickness and marginal bone loss around dental implants with sloped micro-threaded shoulder (35° angle) in comparing with conventional design, inserted 35° degrees angulated in post extraction sockets and immediate loaded with temporary prosthesis simulating the all-on-four protocol. Materials and Methods: Six fox hound dogs received forty-eight post extraction dental implants with the same diameter and length (Medentika, Germany), but with different neck configurations...
January 12, 2018: Materials
https://www.readbyqxmd.com/read/29328159/adaptive-compensation-of-intracavity-tilts-during-lasing-through-detecting-the-direction-of-output-beams
#7
Boheng Lai, Lizhi Dong, Xin Yu, Kangjian Yang, Shanqiu Chen, Shuai Wang, Xing He, Bing Xu, Ping Yang
We present adaptive compensation of intracavity tilts based on detecting the direction of the output beams. We use the initial direction of the output beam when the laser cavity is well collimated for reference. Then the difference between the actual direction of the output beam and the reference is used as feedback to control the intracavity tip/tilt mirror. The relation between the direction of the output beam and the intracavity tilt is investigated with both the Fox-Li method and measurements. A series of experiments demonstrate that intracavity tilts can be well compensated by the proposed method...
January 10, 2018: Applied Optics
https://www.readbyqxmd.com/read/29327343/changes-in-out-of-pocket-charges-associated-with-obstetric-care-provided-under-medicare-in-australia
#8
Emily Callander, Haylee Fox
Recent health reforms alongside unregulated provider fees have led to increased attention being given to out-of-pocket healthcare costs. This study utilised annual statistics published by the Department of Health for services provided under the Medicare Benefits Schedule (MBS) from 1992/3 to 2016/17 to identify changes in out-of-pocket charges for obstetric items over time, and estimate the change in demand for obstetric items in response to price increases. Since 1992/3 out-of-pocket charges increased by 1035% for out-of-hospital items and 77% for in-hospital items...
January 12, 2018: Australian & New Zealand Journal of Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/29327326/il-11-facilitates-a-novel-connection-between-ra-joint-fibroblasts-and-endothelial-cells
#9
Hatem A Elshabrawy, Michael V Volin, Abdul B Essani, Zhenlong Chen, Iain B McInnes, Katrien Van Raemdonck, Karol Palasiewicz, Shiva Arami, Mark Gonzalez, Hossam M Ashour, Seung-Jae Kim, Guofei Zhou, David A Fox, Shiva Shahrara
IL-11 has been detected in inflamed joints; however, its role in the pathogenesis of arthritis is not yet clear. Studies were conducted to characterize the expression and functional significance of IL-11 and IL-11Rα in rheumatoid arthritis (RA). IL-11 levels were elevated in RA synovial fluid (SF) compared to osteoarthritis (OA) SF and plasma from RA, OA and normal individuals (NLs). Morphologic studies established that IL-11 was detected in lining fibroblasts and macrophages in addition to sublining endothelial cells and macrophages at higher levels in RA compared to NL synovial tissues...
January 11, 2018: Angiogenesis
https://www.readbyqxmd.com/read/29327240/geriatric-oncology-in-spain-survey-results-and-analysis-of-the-current-situation
#10
R Gironés, I Morilla, C Guillen-Ponce, M D Torregrosa, I Paredero, E Bustamante, S Del Barco, G Soler, B Losada, L Visa, E Llabrés, B Fox, J L Firvida, R Blanco, M Antonio, F Aparisi, M Pi-Figueras, E Gonzalez-Flores, M J Molina-Garrido, J Saldaña
INTRODUCTION: Geriatric oncology (GO) is a discipline that focuses on the management of elderly patients with cancer. The Spanish Society of Medical Oncology (SEOM) created a Working group dedicated to geriatric oncology in February 2016. OBJECTIVES: The main goal of this study was to describe the current situation in Spain regarding the management of elderly cancer patients through an online survey of medical oncologists. METHODS: A descriptive survey was sent to several hospitals by means of the SEOM website...
December 11, 2017: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/29325878/can-we-identify-futility-in-kids-an-evaluation-of-admission-parameters-predicting-100-mortality-in-1292-severely-injured-children
#11
Kyle J Kalkwarf, Shane D Jensen, Myron Allukian, Matthew T Harting, Charles S Cox, Erin E Fox, Charles E Wade, Bryan A Cotton
BACKGROUND: Objective parameters predicting futility of care in severely injured pediatric patients are lacking. While futility of care has been investigated in a limited number of studies in trauma patients, none of these studies achieves a 100% success rate in a large cohort of pediatric patients. The purpose of the current study was to identify extreme laboratory values that could be used to predict 100% mortality in severely injured children. STUDY DESIGN: Registry-based, historical cohort of all severely injured children (level 1 trauma, <16 years old), who were not dead on arrival, between January 2010 and December 2016 from a single level 1 trauma center...
January 8, 2018: Journal of the American College of Surgeons
https://www.readbyqxmd.com/read/29325820/an-assessment-of-shedding-with-the-oral-rabies-virus-vaccine-strain-spbn-gasgas-in-target-and-non-target-species
#12
Ad Vos, Conrad Freuling, Steffen Ortmann, Antje Kretzschmar, Dietmar Mayer, Annette Schliephake, Thomas Müller
A safety requirement for live vaccines is investigating possible shedding in recipients since the presence of replication competent vaccine in secretions could result in direct and indirect horizontal transmission. This is especially relevant for oral rabies vaccine baits that are deliberately distributed into the environment. In the current study, survival of an oral rabies virus vaccine, SPBN GASGAS, was examined in excretions from different target and non-target species; red fox, raccoon dog, small Indian mongoose, raccoon, striped skunk, domestic dog, domestic cat and domestic pig...
January 8, 2018: Vaccine
https://www.readbyqxmd.com/read/29324991/orbita-revisited-what-it-really-means-and-what-it-does-not
#13
Bernard R Chaitman, Maria Mori Brooks, Kim Fox, Thomas F Lüscher
No abstract text is available yet for this article.
January 6, 2018: European Heart Journal
https://www.readbyqxmd.com/read/29324609/outcomes-of-planned-compared-with-urgent-deliveries-using-a-multidisciplinary-team-approach-for-morbidly-adherent-placenta
#14
Alireza A Shamshirsaz, Karin A Fox, Hadi Erfani, Steven L Clark, Amir A Shamshirsaz, Ahmed A Nassr, Nathan C Sundgren, Jeffery A Jones, Matthew L Anderson, Elias Kassir, Bahram Salmanian, Alexandra W Buffie, Shiu-Ki Hui, Jimmy Espinoza, Lynda A Tyer-Viola, Martha Rac, Niloofar Karbasian, Jerasimos Ballas, Gary A Dildy, Michael A Belfort
OBJECTIVE: To compare outcomes between planned and urgent cesarean hysterectomy for morbidly adherent placenta managed by a multidisciplinary team. METHODS: This is a retrospective case-control study of women with singleton pregnancies with antenatally suspected and pathologically confirmed morbidly adherent placenta who underwent cesarean hysterectomy between January 1, 2011, and February 30, 2017. Timing of delivery was classified as either planned (delivery at 34-35 weeks of gestation) or urgent (need for urgent delivery as a result of uterine contractions, bleeding, or both)...
January 9, 2018: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29324464/sacubitril-valsartan-associated-small-bowel-ileus
#15
Lilianna Hanefeld-Fox, Lissette Jimenez, Rebecca Heflin, Luis Quiel
No abstract text is available yet for this article.
January 8, 2018: American Journal of Therapeutics
https://www.readbyqxmd.com/read/29322198/association-of-cardiovascular-biomarkers-with-incident-heart-failure-with-preserved-and-reduced-ejection-fraction
#16
Rudolf A de Boer, Matthew Nayor, Christopher R deFilippi, Danielle Enserro, Vijeta Bhambhani, Jorge R Kizer, Michael J Blaha, Frank P Brouwers, Mary Cushman, Joao A C Lima, Hossein Bahrami, Pim van der Harst, Thomas J Wang, Ron T Gansevoort, Caroline S Fox, Hanna K Gaggin, Willem J Kop, Kiang Liu, Ramachandran S Vasan, Bruce M Psaty, Douglas S Lee, Hans L Hillege, Traci M Bartz, Emelia J Benjamin, Cheeling Chan, Matthew Allison, Julius M Gardin, James L Januzzi, Sanjiv J Shah, Daniel Levy, David M Herrington, Martin G Larson, Wiek H van Gilst, John S Gottdiener, Alain G Bertoni, Jennifer E Ho
Importance: Nearly half of all patients with heart failure have preserved ejection fraction (HFpEF) as opposed to reduced ejection fraction (HFrEF), yet associations of biomarkers with future heart failure subtype are incompletely understood. Objective: To evaluate the associations of 12 cardiovascular biomarkers with incident HFpEF vs HFrEF among adults from the general population. Design, Setting, and Participants: This study included 4 longitudinal community-based cohorts: the Cardiovascular Health Study (1989-1990; 1992-1993 for supplemental African-American cohort), the Framingham Heart Study (1995-1998), the Multi-Ethnic Study of Atherosclerosis (2000-2002), and the Prevention of Renal and Vascular End-stage Disease study (1997-1998)...
January 10, 2018: JAMA Cardiology
https://www.readbyqxmd.com/read/29321909/detecting-rare-carnivores-using-scats-implications-for-monitoring-a-fox-incursion-into-tasmania
#17
David S L Ramsey, Candida Barclay, Catriona D Campbell, Elise Dewar, Anna J MacDonald, Elodie Modave, Sumaiya Quasim, Stephen D Sarre
The ability to detect the incursion of an invasive species or destroy the last individuals during an eradication program are some of the most difficult aspects of invasive species management. The presence of foxes in Tasmania is a contentious issue with recent structured monitoring efforts, involving collection of carnivore scats and testing for fox DNA, failing to detect any evidence of foxes. Understanding the likelihood that monitoring efforts would detect fox presence, given at least one is present, is therefore critical for understanding the role of scat monitoring for informing the response to an incursion...
January 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29321894/development-of-a-genotype-by-sequencing-immunogenetic-assay-as-exemplified-by-screening-for-variation-in-red-fox-with-and-without-endemic-rabies-exposure
#18
Michael E Donaldson, Yessica Rico, Karsten Hueffer, Halie M Rando, Anna V Kukekova, Christopher J Kyle
Pathogens are recognized as major drivers of local adaptation in wildlife systems. By determining which gene variants are favored in local interactions among populations with and without disease, spatially explicit adaptive responses to pathogens can be elucidated. Much of our current understanding of host responses to disease comes from a small number of genes associated with an immune response. High-throughput sequencing (HTS) technologies, such as genotype-by-sequencing (GBS), facilitate expanded explorations of genomic variation among populations...
January 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29321249/facing-each-other-mammal-mothers-and-infants-prefer-the-position-favouring-right-hemisphere-processing
#19
Andrey Giljov, Karina Karenina, Yegor Malashichev
The right hemisphere plays a crucial role in social processing. Human mothers show a robust left cradling/holding bias providing greater right-hemispheric involvement in the exchange of social information between mother and infant. Here, we demonstrate that a similar bias is evident in face-to-face spatial interactions in marine and terrestrial non-primate mammals. Walruses and Indian flying foxes showed a significant population-level preference for the position which facilitates the use of the left visual field in both mother and infant...
January 2018: Biology Letters
https://www.readbyqxmd.com/read/29318487/dpf-soot-as-an-adsorbent-for-cu-ii-cd-ii-and-cr-vi-compared-with-commercial-activated-carbon
#20
Kun Yang, John Fox
The use of carbon soot recovered from diesel particulate filters (DPF) has been investigated as a potential adsorbent for heavy metals including cadmium, chromium, and copper from wastewater. Results were compared with the adsorption performance of powder activated carbon (PAC). The uptake capacity of heavy metals for soot was found to be higher than PAC. And the thermodynamic study result for both soot and PAC indicated the adsorption procedures are exothermic. The adsorption studies were carried out for both single and binary systems...
January 9, 2018: Environmental Science and Pollution Research International
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