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https://www.readbyqxmd.com/read/28527512/protective-effect-of-capsaicin-against-methyl-methanesulphonate-induced-toxicity-in-the-third-instar-larvae-of-transgenic-drosophila-melanogaster-hsp70-lacz-bg-9
#1
Saba Khanam, Ambreen Fatima, Rahul Smita Jyoti, Fahad Ali, Falaq Naz, Barkha Shakya, Yasir Hasan Siddique
Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the main component in hot peppers, including red chili peppers, jalapenos, and habanero, belonging to the genus Capsicum. Capsaicin is a potent antioxidant that interferes with free radical activities. In the present study, the possible protective effect of capsaicin was studied against methyl methanesulphonate (MMS) induced toxicity in third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg(9). The third instar was allowed to feed on the diet having different doses of capsaicin and MMS separately and in combination...
April 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28523530/bag-1l-protects-sh-sy5y-neuroblastoma-cells-against-hypoxia-re-oxygenation-through-up-regulating-hsp70-and-activating-pi3k-akt-signaling-pathway
#2
Yun Wang, Chao Jia, Qing-Shu Li, Chun-Yu Xie, Nan Zhang, Yan Qu
BCL-2-associated athanogene-1(BAG-1) is a multifunctional and anti-apoptotic protein that was first identified as a binding partner of BCL-2. But the effects and mechanisms for BAG-1 against hypoxic damage is unclear up to now. Whether BAG-1 could protect the human brain against hypoxic damage through up-regulating 70 kDa heat shock proteins (HSP70) and PI3K/AKT pathway activation? In present study, we examined the changes of HSP70 and AKT and p-AKT protein level in SH-SY5Y cells with BAG-1L gene over-expression subjected to hypoxia/re-oxygenation injury...
May 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28521848/impacts-of-stocking-density-on-development-and-puberty-attainment-of-replacement-beef-heifers
#3
K M Schubach, R F Cooke, A P Brandão, K D Lippolis, L G T Silva, R S Marques, D W Bohnert
In all, 60 Angus×Hereford heifers were ranked by age and BW (210±2 days and 220±2 kg) on day 0, and assigned to: (a) one of three drylot pens (10×14 m pens; 10 heifers/pen) resulting in a stocking density of 14 m2/heifer (HIDENS; n=3), or (b) one of three pastures (25 ha pastures; 10 heifers/pasture), resulting in a stocking density of 25 000 m2/heifer (LOWDENS; n=3). Pastures were harvested for hay before the beginning of this experiment, and negligible forage was available for grazing to LOWDENS heifers during the experiment (days 0 to 182)...
May 19, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28520897/alpha-linolenic-acid-protects-the-developmental-capacity-of-bovine-cumulus-oocyte-complexes-matured-under-lipotoxic-conditions-in-vitro
#4
W F A Marei, J De Bie, O Mohey-Elsaeed, E Wydooghe, P E J Bols, J L M R Leroy
Elevated concentrations of free fatty acids (FFAs), predominantly palmitic, stearic and oleic acids (PSO), exert detrimental effects on oocyte developmental competence. This study examined the effects of omega-3 alpha-linolenic acid (ALA) during in vitro oocyte maturation (IVM) in the presence of PSO on subsequent embryo development and quality, and the cellular mechanisms that might be involved. Bovine cumulus oocyte complexes (COCs) were supplemented during IVM with ALA (50 μM), PSO (425 μM), or PSO+ALA...
May 18, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28511856/measurement-of-15-n-longitudinal-relaxation-rates-in-15-nh4-spin-systems-to-characterise-rotational-correlation-times-and-chemical-exchange
#5
D Flemming Hansen
Many chemical and biological processes rely on the movement of monovalent cations and an understanding of such processes can therefore only be achieved by characterising the dynamics of the involved ions. It has recently been shown that (15)N-ammonium can be used as a proxy for potassium to probe potassium binding in bio-molecules such as DNA quadruplexes and enzymes. Moreover, equations have been derived to describe the time-evolution of (15)N-based spin density operator elements of (15)NH4(+) spin systems...
June 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28510570/bag-1-as-a-biomarker-in-early-breast-cancer-prognosis-a-systematic-review-with-meta-analyses
#6
E S Papadakis, T Reeves, N H Robson, T Maishman, G Packham, R I Cutress
BACKGROUND: The co-chaperone protein Bcl-2-associated athanogene-1 (BAG-1) is overexpressed in breast cancer and has been incorporated in the oncotype DX and PAM50 breast cancer prognostic assays. Bcl-2-associated athanogene-1 exists as multiple protein isoforms that interact with diverse partners, including chaperones Hsc70/Hsp70, Ser/Thr kinase Raf-1 and Bcl-2, to promote cancer cell survival. The BAG-1L isoform specifically binds to and increases the transcriptional activity of oestrogen receptor in cells, and in some, but not all studies, BAG-1 expression is predictive of clinical outcome in breast cancer...
May 16, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28510050/a-review-of-multi-domain-and-flexible-molecular-chaperones-studies-by-small-angle-x-ray-scattering
#7
REVIEW
Júlio C Borges, Thiago V Seraphim, Paulo R Dores-Silva, Leandro R S Barbosa
Intrinsic flexibility is closely related to protein function, and a plethora of important regulatory proteins have been found to be flexible, multi-domain or even intrinsically disordered. On the one hand, understanding such systems depends on how these proteins behave in solution. On the other, small-angle X-ray scattering (SAXS) is a technique that fulfills the requirements to study protein structure and dynamics relatively quickly with few experimental limitations. Molecular chaperones from Hsp70 and Hsp90 families are multi-domain proteins containing flexible and/or disordered regions that play central roles in cellular proteostasis...
June 2016: Biophysical Reviews
https://www.readbyqxmd.com/read/28505454/perturbation-response-scanning-reveals-key-residues-for-allosteric-control-in-hsp70
#8
David Penkler, Ozge Sensoy, Canan Atilgan, Ozlem Tastan Bishop
Hsp70 molecular chaperones play an important role in maintaining cellular homeostasis, and are implicated in a wide array of cellular processes including protein recovery from aggregates, cross membrane protein translocation, and protein biogenesis. Hsp70 consists of two domains, a nucleotide binding domain (NBD) and a substrate binding domain (SBD), each of which communicates via an allosteric mechanism such that the protein interconverts between two functional states, an ATP bound open conformation and an ADP bound closed conformation...
May 15, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28504929/evolution-of-an-intricate-j-protein-network-driving-protein-disaggregation-in-eukaryotes
#9
Nadinath B Nillegoda, Antonia Stank, Duccio Malinverni, Niels Alberts, Anna Szlachcic, Alessandro Barducci, Paolo De Los Rios, Rebecca C Wade, Bernd Bukau
Hsp70 participates in a broad spectrum of protein folding processes extending from nascent chain folding to protein disaggregation. This versatility in function is achieved through a diverse family of J-protein cochaperones that select substrates for Hsp70. Substrate selection is further tuned by transient complexation between different classes of J-proteins, which expands the range of protein aggregates targeted by metazoan Hsp70 for disaggregation. We assessed the prevalence and evolutionary conservation of J-protein complexation and cooperation in disaggregation...
May 15, 2017: ELife
https://www.readbyqxmd.com/read/28504607/innate-inflammatory-gene-expression-profiling-in-potential-brain-dead-donors-detailed-investigation-of-the-effect-of-common-corticosteroid-therapy
#10
Reza Gholamnezhadjafari, Nader Tajik, Reza Falak, Reza Aflatoonian, Sanaz Dehghan, Abbas Rezaei
Our study aimed to assess the influence of common methylprednisolone therapy on innate inflammatory factors in potential brain-dead organ donors (BDDs). The study groups consisted of 50 potential BDDs who received 15 mg/kg/d methylprednisolone and 25 live organ donors (LDs) as control group. Innate immunity gene expression profiling was performed by RT-PCR array. Soluble serum cytokines and chemokines, complement components, heat shock protein 70 (HSP70) and high mobility group box-1 (HMGB1) were measured by ELISA...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/28504248/the-flavonoid-tl-2-8-induces-cell-death-and-immature-mitophagy-in-breast-cancer-cells-via-abrogating-the-function-of-the-aha1-hsp90-complex
#11
Hui-Juan Liu, Xiao-Xiao Jiang, Yi-Zhen Guo, Fang-Hui Sun, Xin-Hui Kou, Yong Bao, Zhu-Qing Zhang, Zhao-Hu Lin, Ting-Bo Ding, Lan Jiang, Xin-Sheng Lei, Yong-Hua Yang
The flavonoid quercetin exhibits significant anticancer activities with few side effects. In the current study, we characterized TL-2-8, a quercetin derivative, as a novel anticancer agent in vitro and in vivo. Cell proliferation and viability were assessed using Cell Counting Kit-8 and CellTiter-Blue assay, respectively. Cell death was examined using PI staining or a TUNEL assay. Mitophagy was determined by measuring autophagic flux and by confocal imaging. Protein expression was examined by Western blotting...
May 1, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28503893/a-comparison-of-two-sources-of-methionine-supplemented-at-different-levels-on-heat-shock-protein-70-expression-and-oxidative-stress-product-of-peking-ducks-subjected-to-heat-stress
#12
L Guo, R Li, Y F Zhang, T Y Qin, Q S Li, X X Li, Z L Qi
The aim of this study was to examine the effects of different sources and levels of methionine (Met) on Heat shock proteins HSP70 expression and protein carbonylation in liver, HSP70 expression and malondialdehyde (MDA) concentration in intestine under heat stress conditions during summer. A total of 720 (4 days old) Peking ducks were placed 20 per pen into six replicates for each of the six treatments with a 2 × 3 factorial arrangement, such that two sources of Met (DL-methionine [DLM] and DL-2-hydroxy-4-methylthiobutyrate [HMTBA] were supplemented at three different levels (0...
May 15, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28502644/leishmania-v-braziliensis-infecting-bats-from-pantanal-wetland-brazil-first-records-for-platyrrhinus-lineatus-and-artibeus-planirostris
#13
Eduardo de Castro Ferreira, Agnes Antônio Sampaio Pereira, Maurício Silveira, Carina Margonari, Glaucia Elisete Barbosa Marcon, Adriana de Oliveira França, Ludiele Souza Castro, Marcelo Oscar Bordignon, Erich Fischer, Walfrido Moraes Tomas, Maria Elizabeth Cavalheiros Dorval, Célia Maria Ferreira Gontijo
In the New World genus Leishmania parasites are etiological agents of neglected zoonoses known as leishmaniasis. Its epidemiology is very complex due to the participation of several species of sand fly vectors and mammalian hosts, and man is an accidental host. Control is very difficult because of the different epidemiological patterns of transmission observed. Studies about Leishmania spp. infection in bats are so scarce, which represents a large gap in knowledge about the role of these animals in the transmission cycle of these pathogens, especially when considering that Chiroptera is one of the most abundant and diverse orders among mammals...
May 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28501483/reversal-of-bioenergetics-dysfunction-by-diphenyl-diselenide-is-critical-to-protection-against-the-acetaminophen-induced-acute-liver-failure
#14
Nélson R Carvalho, Cintia C Tassi, Fernando Dobraschinski, Guilherme P Amaral, Ana P Zemolin, Ronaldo M Golombieski, Cristiane L Dalla Corte, Jeferson L Franco, José L Mauriz, Javier González-Gallego, Félix A Soares
Physiopathological conditions such as acute liver failure (ALF) induced by acetaminophen (APAP) can often impair the mitochondrial bioenergetics. Diphenyl diselenide [(PhSe)2] has been shown protects against APAP-induced ALF. The present study aimed to clarify the signaling mechanism involved in the protection of bioenergetics dysfunction associated with ALF-induced by APAP overdose. Mice received APAP (600mg/kg) or (PhSe)2 (15.6mg/kg) alone, or APAP+(PhSe)2, all the solutions were administered by the intraperitoneal (i...
May 10, 2017: Life Sciences
https://www.readbyqxmd.com/read/28500894/mercury-induced-oxidative-stress-dna-damage-and-activation-of-antioxidative-system-and-hsp70-induction-in-duckweed-lemna-minor
#15
Tingting Zhang, Qianqian Lu, Chunlei Su, Yaru Yang, Dan Hu, Qinsong Xu
Mercury uptake and its effects on physiology, biochemistry and genomic stability were investigated in Lemna minor after 2 and 6d of exposure to 0-30μM Hg. The accumulation of Hg increased in a concentration- and duration-dependent manner, and was positively correlated with the leaf damage. Oxidative stress after Hg exposure was evidenced in L. minor by a significant decrease in photosynthetic pigments, an increase in malondialdehyde and lipoxygenase activities (total enzyme activity and isoenzymes activity)...
May 10, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28498104/modeling-hsp70-hsp40-interaction-by-multi-scale-molecular-simulations-and-co-evolutionary-sequence-analysis
#16
Duccio Malinverni, Alfredo Jost Lopez, Paolo De Los Rios, Gerhard Hummer, Alessandro Barducci
The interaction between the Heat Shock Proteins 70 and 40 is at the core of the ATPase regulation of the chaperone machinery that maintains protein homeostasis. However, the structural details of the interaction are still elusive and contrasting models have been proposed for the transient Hsp70/Hsp40 complexes. Here we combine molecular simulations based on both coarse-grained and atomistic models with co-evolutionary sequence analysis to shed light on this problem by focusing on the bacterial DnaK/DnaJ system...
May 12, 2017: ELife
https://www.readbyqxmd.com/read/28497897/expression-profile-of-heat-shock-proteins-in-placental-tissues-of-patients-with-preterm-prelabor-rupture-of-membranes-and-spontaneous-preterm-labor-with-intact-membranes
#17
Lenka Dvorakova, Katarina Ivankova, Ladislav Krofta, Ilona Hromadnikova
PROBLEM: Investigating the stress response in the central cotyledon zone of placental tissue in pregnancies with PPROM, PTB, and at term in labor. METHOD OF STUDY: Gene expression of Hsp27, Hsp60, Hsp70, Hsp90, and HspBP1 was compared between these particular groups. Correlation between variables including Hsp gene expression in placental tissue and the gestational age at delivery, WBC count at admission, and serum levels of CRP at admission in patients with PPROM and PTB was determined...
May 12, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28497562/different-aggregation-states-of-a-nuclear-localization-signal-tagged-25-kda-c-terminal-fragment-of-tar-rna-dna-binding-protein-43%C3%A2-kda
#18
Akira Kitamura, Sachiko Yuno, Hideki Muto, Masataka Kinjo
The mechanism and cause of motor neuronal cell death in amyotrophic lateral sclerosis (ALS), a devastating neurodegenerative disorder, are unknown; gain of function of oligomers and aggregation of misfolded proteins, including carboxyl-terminal fragments (CTFs) of TAR RNA/DNA-binding protein 43 kDa (TDP-43), have been proposed as important causative factors in the onset of ALS. We recently reported that a nuclear localization signal (NLS)-tagged 25-kDa CTF of TDP-43 (TDP25) could decrease the cell-death proportion compared with that promoted by TDP25...
May 12, 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/28497369/co-enzyme-q10-and-acetyl-salicylic-acid-enhance-hsp70-expression-in-primary-chicken-myocardial-cells-to-protect-the-cells-during-heat-stress
#19
Jiao Xu, Shu Tang, Bin Yin, Jiarui Sun, Erbao Song, Endong Bao
We investigated the effects of co-enzyme Q10 (Q10) and acetyl salicylic acid (ASA) on expression of Hsp70 in the protection of primary chicken myocardial cells during heat stress. Western blot analysis showed that Q10 and ASA accelerated the induction of Hsp70 when chicken myocardial cells were exposed to hyperthermia. In the absence of heat stress, however, neither Q10 nor ASA are able to upregulate Hsp70 expression. Analysis of enzymes that respond to cellular damage and pathological examination revealed that ectopic expression of ASA and Q10 alleviate cellular damage during heat stress...
May 11, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28496305/methanol-leaf-extract-of-actinodaphne-sesquipedalis-lauraceae-enhances-gastric-defense-against-ethanol-induced-ulcer-in-rats
#20
Hanita Omar, Noraziah Nordin, Pouya Hassandarvish, Maryam Hajrezaie, Ainnul Hamidah Syahadah Azizan, Mehran Fadaeinasab, Nazia Abdul Majid, Mahmood Ameen Abdulla, Najihah Mohd Hashim, Hapipah Mohd Ali
Actinodaphne sesquipedalis Hook. F. Var. Glabra (Kochummen), also known as "Medang payung" by the Malay people, belongs to the Lauraceae family. In this study, methanol leaf extract of A. sesquipedalis was investigated for their acute toxicity and gastroprotective effects to reduce ulcers in rat stomachs induced by ethanol. The rats were assigned to one of five groups: normal group (group 1), ulcer group (group 2), control positive drug group (group 3) and two experimental groups treated with 150 mg/kg (group 4) and 300 mg/kg (group 5) of leaf extract...
2017: Drug Design, Development and Therapy
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