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https://www.readbyqxmd.com/read/29330519/large-scale-aggregation-analysis-of-eukaryotic-proteins-reveals-an-involvement-of-intrinsically-disordered-regions-in-protein-folding
#1
Eri Uemura, Tatsuya Niwa, Shintaro Minami, Kazuhiro Takemoto, Satoshi Fukuchi, Kodai Machida, Hiroaki Imataka, Takuya Ueda, Motonori Ota, Hideki Taguchi
A subset of the proteome is prone to aggregate formation, which is prevented by chaperones in the cell. To investigate whether the basic principle underlying the aggregation process is common in prokaryotes and eukaryotes, we conducted a large-scale aggregation analysis of ~500 cytosolic budding yeast proteins using a chaperone-free reconstituted translation system, and compared the obtained data with that of ~3,000 Escherichia coli proteins reported previously. Although the physicochemical properties affecting the aggregation propensity were generally similar in yeast and E...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329610/bacopa-monnieri-extracts-prevent-hydrogen-peroxide-induced-oxidative-damage-in-a-cellular-model-of-neuroblastoma-imr32-cells
#2
Gaurav Bhatia, Vikram Dhuna, Kshitija Dhuna, Manpreet Kaur, Jatinder Singh
Neurodegenerative diseases are the consequences of imbalance between the production of oxidative stress and its nullification by cellular defense mechanisms. Hydrogen peroxide (H2O2), a precursor of deleterious reactive oxygen species, elicits oxidative stress, resulting in severe brain injuries. Bacopa monnieri is well known for its nerve relaxing and memory enhancing properties. The present study was designed to evaluate the protective effects of extracts from Bacopa monnieri against H2O2 induced oxidative stress using a cellular model, neuroblastoma IMR32 cell line...
November 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29325453/age-related-lung-tissue-remodeling-due-to-the-local-distribution-of-mmp-2-timp-2-tgf-%C3%AE-and-hsp70
#3
Z Vitenberga, M Pilmane
Lung tissue remodeling requires complex interactions of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), transforming growth factor (TGF) family and heat shock protein 70 (Hsp70). We evaluated the appearance and distribution of MMP-2, TIMP-2, TGF-β1 and Hsp70 in lung tissue using immunohistochemistry. Stained structures were graded semiquantitatively. Overall, more MMP-2, TIMP-2, TGF-β1 and Hsp70 were observed in bronchial cartilage, bronchial and alveola repithelium, and among alveolar macrophages...
January 12, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29323700/heat-shock-protein-hsp-regulation-by-muscarinic-acetylcholine-receptor-machr-activation-in-the-rat-hippocampus
#4
Frinchi Monica, Scaduto Pietro, Cappello Francesco, Belluardo Natale, Mudò Giuseppa
The cholinergic system plays a crucial role in modulating in the central nervous system physiological responses such as neurogenesis, neuronal differentiation, synaptic plasticity and neuroprotection. In a recent study, we showed that Oxotremorine-M, a non-selective muscarinic acetylcholine receptor agonist, is able to transactivate the fibroblast growth factor receptor and to produce a significant increase in the hippocampal primary neurite outgrowth. In the present study we aimed to explore in the rat hippocampus the possible effect of acute or chronic treatment with Oxotremorine-M on some heat shock proteins (Hsp60, Hsp70, Hsp90) and on activation of related transcription factor heat shock factor 1 (HSF1)...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29323281/bap-sil1-regulates-the-molecular-chaperone-bip-by-coupling-release-of-nucleotide-and-substrate
#5
Mathias Rosam, Daniela Krader, Christina Nickels, Janine Hochmair, Katrin C Back, Ganesh Agam, Anders Barth, Cathleen Zeymer, Jelle Hendrix, Markus Schneider, Iris Antes, Jochen Reinstein, Don C Lamb, Johannes Buchner
BiP is the endoplasmic member of the Hsp70 family. BiP is regulated by several co-chaperones including the nucleotide-exchange factor (NEF) Bap (Sil1 in yeast). Bap is a two-domain protein. The interaction of the Bap C-terminal domain with the BiP ATPase domain is sufficient for its weak NEF activity. However, stimulation of the BiP ATPase activity requires full-length Bap, suggesting a complex interplay of these two factors. Here, single-molecule FRET experiments with mammalian proteins reveal that Bap affects the conformation of both BiP domains, including the lid subdomain, which is important for substrate binding...
January 2018: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/29323280/nucleotide-exchange-factors-fes1-and-hspbp1-mimic-substrate-to-release-misfolded-proteins-from-hsp70
#6
Naveen K C Gowda, Jayasankar M Kaimal, Roman Kityk, Chammiran Daniel, Jobst Liebau, Marie Öhman, Matthias P Mayer, Claes Andréasson
Protein quality control depends on the tight regulation of interactions between molecular chaperones and polypeptide substrates. Substrate release from the chaperone Hsp70 is triggered by nucleotide-exchange factors (NEFs) that control folding and degradation fates via poorly understood mechanisms. We found that the armadillo-type NEFs budding yeast Fes1 and its human homolog HspBP1 employ flexible N-terminal release domains (RDs) with substrate-mimicking properties to ensure the efficient release of persistent substrates from Hsp70...
January 2018: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/29323151/downregulation-of-heat-shock-protein-70-impairs-osteogenic-and-chondrogenic-differentiation-in-human-mesenchymal-stem-cells
#7
Chenghai Li, Kristifor Sunderic, Steven B Nicoll, Sihong Wang
Human mesenchymal stem cells (hMSCs) show promise for bone and cartilage regeneration. Our previous studies demonstrated that hMSCs with periodic mild heating had enhanced osteogenic and chondrogenic differentiation with significantly upregulated heat shock protein 70 (HSP70). However, the role of HSP70 in adult tissue regeneration is not well studied. Here, we revealed an essential regulatory mechanism of HSP70 in osteogenesis and chondrogenesis using adult hMSCs stably transfected with specific shRNAs to knockdown HSP70...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29323127/differential-proteomics-of-lesional-vs-non-lesional-biopsies-revealed-non-immune-mechanisms-of-alopecia-areata
#8
Kanchalit Thanomkitti, Rattiyaporn Kanlaya, Kedsarin Fong-Ngern, Chompunoot Kapincharanon, Kanyarat Sueksakit, Prangwalai Chanchaem, Rattapon Thuangtong, Visith Thongboonkerd
Alopecia areata (AA) is one of the common hair disorders for which treatment is frequently ineffective and associated with relapsing episodes. Better understanding of disease mechanisms and novel therapeutic targets are thus required. From 10 AA patients, quantitative proteomics using LTQ-Orbitrap-XL mass spectrometer revealed 104 down-regulated, 4 absent, 3 up-regulated and 11 newly present proteins in lesional vs. non-lesional biopsies. Among these, the decreased levels of α-tubulin, vimentin, heat shock protein 70 (HSP70), HSP90, annexin A2 and α-enolase were successfully confirmed by Western blotting...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29317827/optimal-management-of-cogan-s-syndrome-a-multidisciplinary-approach
#9
REVIEW
Vittorio D'Aguanno, Massimo Ralli, Marco de Vincentiis, Antonio Greco
Cogan's syndrome (CS) is a rare disorder characterized by nonsyphilitic interstitial keratitis (IK) and audio-vestibular symptoms. CS affects mainly young Caucasian adults, mostly during their first three decades of age, and may develop into typical and atypical variants. Typical CS manifests primarily with IK and hearing loss, whereas atypical CS usually presents with inflammatory ocular manifestations in association with audio-vestibular symptoms but mostly different Ménière-like symptoms and, more frequently, with systemic inflammation (70%), of which vasculitis is the pathogenic mechanism...
2018: Journal of Multidisciplinary Healthcare
https://www.readbyqxmd.com/read/29313594/effects-of-different-rearing-temperatures-on-muscle-development-and-stress-response-in-the-early-larval-stages-of-acipenser-baerii
#10
Lucia Aidos, Luisa M Pinheiro Valente, Vera Sousa, Marco Lanfranchi, Cinzia Domeneghini, Alessia Di Giancamillo
The present study aims at investigating muscle development and stress response in early stages of Siberian sturgeon when subjected to different rearing temperatures, by analysing growth and development of the muscle and by assessing the stress response of yolk-sac larvae. Siberian sturgeon larvae were reared at 16°C, 19°C and 22°C until the yolk-sac was completely absorbed. Sampling timepoints were: hatching, schooling and complete yolk-sac absorption stage. Histometrical, histochemical and immunohistochemical analyses were performed in order to characterize muscle growth (total muscle area, TMA; slow muscle area, SMA; fast muscle area, FMA), development (anti-proliferating cell nuclear antigen -PCNA or anticaspase) as well as stress conditions by specific stress biomarkers (heat shock protein 70 or 90, HSP70 or HSP90)...
November 28, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29307045/a-comparison-of-cell-survival-and-heat-shock-protein-expression-after-radiation-in-normal-dermal-fibroblasts-microvascular-endothelial-cells-and-different-head-and-neck-squamous-carcinoma-cell-lines
#11
Dominique Muschter, Fabian Geyer, Richard Bauer, Tobias Ettl, Stephan Schreml, Frank Haubner
OBJECTIVES: Head and neck squamous cell carcinoma (HNSCC) shows increased radioresistance due to the manipulation of homeostatic mechanisms like the heat shock response. This study intended to comparatively analyze effects of ionizing radiation on different HNSCC cell lines (PCI) and normal human dermal fibroblasts (NHFs) and human dermal microvascular endothelial cells (HDMECs) to uncover differences in radiation coping strategies. MATERIALS AND METHODS: Proliferation (BrdU assay), apoptosis (caspase 3/7) and intracellular protein expression of heat shock protein (HSP)-70, and phosphorylated and total HSP27, determined by enzyme-linked immunosorbent assay (ELISA), were analyzed after exposure to increasing doses of ionizing radiation (2, 6, and 12 Gray, Gy)...
January 6, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29304094/positive-associations-between-upregulated-levels-of-stress-induced-phosphoprotein-1-and-matrix-metalloproteinase-9-in-endometriosis-adenomyosis
#12
Hsin-Shih Wang, Chia-Lung Tsai, Pi-Yueh Chang, Angel Chao, Ren-Chin Wu, Shun-Hua Chen, Chin-Jung Wang, Chih-Feng Yen, Yun-Shien Lee, Tzu-Hao Wang
Stress-induced phosphoprotein-1 (STIP1), an adaptor protein that coordinates the functions of HSP70 and HSP90 in protein folding, has been implicated in the development of human gynecologic malignancies. This case-control study investigates STIP1 serum levels and tissue expression in relation to endometriosis/adenomyosis in Taiwanese population. Female patients with surgically confirmed endometriosis/adenomyosis were compared with women free of endometriosis/adenomyosis. Serum STIP1 levels were measured using an enzyme-linked immunosorbent assay and surgical tissues were analyzed by immunohistochemistry...
2018: PloS One
https://www.readbyqxmd.com/read/29303960/lhcsr-expression-under-hsp70-rbcs2-promoter-as-a-strategy-to-increase-productivity-in-microalgae
#13
Federico Perozeni, Giulio Rocco Stella, Matteo Ballottari
Microalgae are unicellular photosynthetic organisms considered as potential alternative sources for biomass, biofuels or high value products. However, limited biomass productivity is commonly experienced in their cultivating system despite their high potential. One of the reasons for this limitation is the high thermal dissipation of the light absorbed by the outer layers of the cultures exposed to high light caused by the activation of a photoprotective mechanism called non-photochemical quenching (NPQ). In the model organism for green algae Chlamydomonas reinhardtii, NPQ is triggered by pigment binding proteins called light-harvesting-complexes-stress-related (LHCSRs), which are over-accumulated in high light...
January 5, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29301687/heat-shock-response-and-shape-regulation-during-newt-tail-regeneration
#14
Elena Radugina, Eleonora Grigoryan
Regenerating newt tail has recently been found to react to hypergravity in a stable and reproducible way - by curving downwards. Such morphogenetic effect of non-specific physical factor applied to a complex structure of an adult animal is a rare phenomenon with unknown molecular basis. For the first steps of unraveling this basis we've chosen heat shock proteins (HSPs) as promising candidates. Morphometrical analysis of tail regeneration was performed in aquarium (control), on substrate (relative hypergravity) and in aquarium under weekly application of heat shock...
January 2018: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29301599/experimental-study-on-the-protection-of-agrimony-extracts-from-different-extracting-methods-against-cerebral-ischemia-reperfusion-injury
#15
Hui-Yuan Zhu, Yu-Long Bie, Jiang Wang, Jing Gao, Bing-Yue Yang, Hai-Tong Wan
Objective To study the protective effect of agrimony extracts from different extracting methods on cerebral ischemia-reperfusion injury in rats, in order to optimize the extraction scheme of agrimony. Methods Male rats were randomly assigned into seven groups: 1. Sham-operated group, 2. Untreated MCAO group (MCAO), 3. Petroleum ether extract of Agrimonia pilosa treated MCAO group (PEA), 4. Ethyl acetate extract of Agrimonia pilosa treated MCAO group (EAEA), 5. Ethanol extract of Agrimonia pilosa treated MCAO group (EEA), 6...
December 30, 2017: Chinese Medical Sciences Journal, Chung-kuo i Hsüeh K'o Hsüeh Tsa Chih
https://www.readbyqxmd.com/read/29301492/effect-of-grm7-polymorphisms-on-the-development-of-noise-induced-hearing-loss-in-chinese-han-workers-a-nested-case-control-study
#16
Peipei Yu, Jie Jiao, Guoshun Chen, Wenhui Zhou, Huanling Zhang, Hui Wu, Yanhong Li, Guizhen Gu, Yuxin Zheng, Yue Yu, Shanfa Yu
BACKGROUND: Noise-induced hearing loss (NIHL) is a complex, irreversible disease caused by the interaction of genetic and environmental factors. In recent years, a great many studies have been done to explore the NIHL susceptibility genes among humans. So far, high powerful detections have been founded that genes of potassium ion channel genes (KCNQ4 and KCNE1), catalase (CAT), protocadherin 15 (PCDH15), myosin 14 (MYH14) and heart shock protein (HSP70) which have been identified in more than one population may be associated with the susceptibility to NIHL...
January 5, 2018: BMC Medical Genetics
https://www.readbyqxmd.com/read/29300467/disrupted-hydrogen-bond-network-and-impaired-atpase-activity-in-an-hsc70-cysteine-mutant
#17
John P O'Donnell, Heather M Marsh, Holger Sondermann, Carolyn Sevier
The ATPase domain of the 70-kD heat shock protein (Hsp70) family members shows a high degree of sequence, structural, and functional homology across species. A broadly conserved residue within the Hsp70 ATPase domain that captured our attention is an unpaired cysteine, positioned proximal to the site of nucleotide binding. Prior studies of several Hsp70 family members show this cysteine is not required for Hsp70 ATPase activity, yet select amino acid replacements of the cysteine can dramatically alter ATP hydrolysis...
January 4, 2018: Biochemistry
https://www.readbyqxmd.com/read/29300447/hsp70-inhibits-the-nucleation-and-elongation-of-tau-and-sequesters-tau-aggregates-with-high-affinity
#18
Franziska Kundel, Suman De, Patrick Flagmeier, Mathew H Horrocks, Magnus Kjaergaard, Sarah L Shammas, Sophie E Jackson, Christopher M Dobson, David Klenerman
As a key player of the protein quality control network of the cell, the molecular chaperone Hsp70 inhibits the aggregation of the amyloid protein tau. To date, the mechanism of this inhibition and the tau species targeted by Hsp70 remain unknown. This is partly due to the inherent difficulty of studying amyloid aggregates because of their heterogeneous and transient nature. Here, we used ensemble and single-molecule fluorescence measurements to dissect how Hsp70 counteracts the self-assembly process of K18 tau with the pathological deletion ∆K280...
January 4, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29299441/effects-of-partial-dietary-supplementation-of-fish-meal-with-soymeal-on-the-stress-and-apoptosis-response-in-the-digestive-system-of-common-dentex-dentex-dentex
#19
Efthimia Antonopoulou, Eleni Chouri, Konstantinos Feidantsis, Antigone Lazou, Stavros Chatzifotis
Background: Soybean is a common alternative protein source of plant origin in aquafeeds as it has a reasonably balanced amino acid profile and is widely available. This study aimed to investigate the influence of partial substitution of fish meal with soy meal on cytoprotective pathways and apoptosis in the digestive system of common dentex (Dentex dentex), using the activation of Hsp70, p38 MAPK, Bcl-2 and caspase-3. The experimental approach involved feeding of common dentex with three isoprotein and isoenergetic diets that contained fish meal as a protein source (FM), partial replacement of fish meal by soy meal 25% (SM25) and 40% (SM40) for 3 months...
December 2017: Journal of Biological Research
https://www.readbyqxmd.com/read/29298131/a-systematic-review-of-p53-regulation-of-oxidative-stress-in-skeletal-muscle
#20
Kaitlyn Beyfuss, David A Hood
BACKGROUND: p53 is a tumor suppressor protein involved in regulating a wide array of signaling pathways. The role of p53 in the cell is determined by the type of imposed oxidative stress, its intensity and duration. The last decade of research has unravelled a dual nature in the function of p53 in mediating the oxidative stress burden. However, this is dependent on the specific properties of the applied stress and thus requires further analysis. METHODS: A systematic review was performed following an electronic search of Pubmed, Google Scholar, and ScienceDirect databases...
January 3, 2018: Redox Report: Communications in Free Radical Research
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