Wade Cavender, Christine Swan, Skylar Wolf, Danielle Van Vliet, Alison Aceves Johnson, Anna Forest, Robert Shields, Thomas Loch, Christopher Knupp, John Drennan, Gavin Glenney, Sascha L Hallett, Joe Marcino, Aimee Reed
OBJECTIVE: We sought to evaluate accurate and reproducible detection of Myxobolus cerebralis (Mc), the causative agent of whirling disease, by using nested polymerase chain reaction (nPCR) and three previously established real-time quantitative PCR (qPCR) assays: K18S (Kelley 18S), C18S (Cavender 18S), and Hsp70 (heat shock protein 70). We used a "fit for purpose" approach combined with intra- and interlaboratory testing to identify a molecular testing method that would be equivalent to the currently accepted nPCR procedure for Mc...
April 15, 2024: Journal of Aquatic Animal Health