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Bone broth

Daniel A Bossard, Bruno Ledergerber, Patrick O Zingg, Christian Gerber, Annelies S Zinkernagel, Reinhard Zbinden, Yvonne Achermann
Diagnosis of Propionibacterium acnes bone and joint infection is challenging due to long cultivation time of up to 14 days. We retrospectively studied whether reducing cultivation time to 7 days allows accurate diagnosis without losing sensitivity. We identified patients with at least one positive P. acnes sample between 2005 and 2015 and grouped into 'infection' and 'no infection'. An 'infection' was defined when at least two samples from the same case were positive. Clinical and microbiological data including time to positivity for different cultivation methods were recorded...
October 12, 2016: Journal of Clinical Microbiology
L Kvich, P Ø Jensen, U S Justesen, T Bjarnsholt
The aim of this study was to prospectively investigate the incidence of Propionibacterium acnes in thioglycollate broths reported as culture-negative at the Department of Clinical Microbiology, Rigshospitalet, to evaluate whether 5 days of incubation was enough to find all relevant cases. Five hundred thioglycollate broths reported as culture-negative after 5 days were consecutively collected and incubated for at least a further 9 days (at least 14 days of incubation in total). Only tissue samples from sterile sites of the body (n = 298), bone tissue (n = 197) and foreign material (n = 5) were included in this study...
August 11, 2016: Clinical Microbiology and Infection
Ian P Goodwin, Ogan K Kumova, Shira Ninio
The OmpA-like protein domain has been associated with peptidoglycan-binding proteins, and is often found in virulence factors of bacterial pathogens. The intracellular pathogen Legionella pneumophila encodes for six proteins that contain the OmpA-like domain, among them the highly conserved uncharacterized protein we named CmpA. Here we set out to characterize the CmpA protein and determine its contribution to intracellular survival of L. pneumophila Secondary structure analysis suggests that CmpA is an inner membrane protein with a peptidoglycan-binding domain at the C-teminus...
August 2016: FEMS Microbiology Letters
Jeong-Tae Yeon, Hiyoung Kim, Kwang-Jin Kim, Jihye Lee, Dong Hwan Won, Sang-Jip Nam, Seong Hwan Kim, Heonjoong Kang, Young-Jin Son
A new inhibitor, acredinone C (1), of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation was isolated from the culture broth of the fungus Acremonium sp. (F9A015) along with acredinones A (2) and B (3). The structure of acredinone C (1), which incorporates benzophenone and xanthone moieties, was established by the analyses of combined spectroscopic data including 1D and 2D NMR and MS. All of the acredinones studied efficiently inhibited the RANKL-induced formation of TRAP(+)-MNCs in a dose-dependent manner without any cytotoxicity up to 10 μM...
July 22, 2016: Journal of Natural Products
Elena De Vecchi, Monica Bortolin, Valentina Signori, Carlo L Romanò, Lorenzo Drago
BACKGROUND: Prosthetic implants, periprosthetic and osteoarticular tissues are specimens of choice for diagnosis of bone and joint infections. Homogenization is considered the best procedure for treatment of tissues samples, but, it is not always performed in all laboratories. Dithiothreitol (DTT) has been proposed as an alternative treatment to sonication for microbiological diagnosis of prosthetic joint infections. In this study, the applicability of DTT treatment for processing of periprosthetic and osteoarticular tissues for diagnosis of bone and joint infections was evaluated and compared with normal saline solution treatment...
May 11, 2016: Journal of Arthroplasty
Kun Hwang, Hyung Mook Kim
The authors experienced a patient of infection necrosis after an extracorporeal reduction of a condyle fracture and reconstructed it with an iliac bone graft.A 37-year-old man visited with a bilateral condyle fracture and a left para-symphyseal fracture. On the post-trauma third day, an intermaxillary fixation was applied using arch bars. On the post-trauma tenth day, an extracorporeal reduction was carried out. On postoperative day (POD) 7, the amount of the drain was 9 cc and the drain was removed. On POD 9, redness and a pus drain were observed on the operation site...
May 2016: Journal of Craniofacial Surgery
T J Keenan, L M Placek, M M Hall, A W Wren
A bioactive glass series (0.42SiO2 -0.10Na2 O-0.08CaO-(0.40 - x)ZnO-(x)Ga2 O3 ) was synthesized, and it is efficacy against the Gram (-ve) bacteria Escherichia coli (E. coli), the Gram (+ve) bacteria Staphylococcus aureus (S. aureus), and the fungus Candida albicans (C. albicans), were characterized through liquid broth analysis. The glass series was also seeded in CMC-Dex hydrogels at three different loadings (0.05, 0.10, and 0.25 m(2) ), and the antibacterial and antifungal efficacies of the resulting composites were characterized using both liquid broth and agar diffusion analysis...
March 21, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Shreya Ghosh, Victoria Wu, Sebastian Pernal, Vuk Uskoković
Osteomyelitis, an infectious disease predominantly tied to poor sanitary conditions in underdeveloped regions of the world, is in need of inexpensive, easily in situ synthesizable and administrable materials for its treatment. The results of this study stem from the attempt to create one such affordable and minimally invasive therapeutic platform in the form of a self-setting, injectable cement with a tunable drug release profile, composed of only nanoparticulate hydroxyapatite, the synthetic version of the bone mineral...
March 2016: ACS Applied Materials & Interfaces
Yu Chen, Da Wu, Min Sun, Mingjun Deng, Shuhua Cui, Chengzhu Liang, Juan Geng, Tao Sun, Ling Long, Xizhi Xiao
While inspecting animal feed for Salmonella contamination, we routinely observed bacterial colonies on selective agars that were similar in appearance to those formed by Salmonella. These were identified as Citrobacter freundii, Proteus mirabilis, and Serratia fonticola using biochemical and serological techniques. Because the presence of these bacterial species confounds identification of Salmonella, we refer to them as "interference bacteria." Polyvalent antisera against these interference bacteria were prepared by immunizing rabbits with a mixture of all three organisms...
January 2016: Journal of AOAC International
Jean-Winoc Decousser, Marine Desroches, Nadège Bourgeois-Nicolaos, Julien Potier, François Jehl, Gérard Lina, Vincent Cattoir, François Vandenesh, Florence Doucet-Populaire
Multiresistance in staphylococci constitutes a major challenge for the antimicrobial chemotherapy of invasive infections such as bacteraemia or bone and joint infections (BJIs). A nationwide prospective study was performed to detect antimicrobial resistance trends among staphylococci causing invasive infections. Between October 2011 and February 2012, 367 meticillin-resistant Staphylococcus aureus (MRSA) and 695 coagulase-negative staphylococci (CoNS) were collected from 37 French hospitals, mainly from bacteraemia (59...
December 2015: International Journal of Antimicrobial Agents
V V Gostev, O S Kalinogorskaya, L N Popenko, T V Chernenkaya, Z S Naumenko, T M Voroshilova, Yu A Zakharova, O E Khokhlova, A N Kruglov, M G Ershova, I V Molchanova, S V Sidorenko
The results of the multicentre trial on estimation of MRSA antibiotic susceptibility to 17 antibiotics are presented. 474 nonrepeting isolates of MRSA (mecA+), collected in 2011-2014 in 10 cities of the Russian Federation were used in the trial. The antibiotic susceptibility was determined by the method of serial microdilutions in broth with estimation of the MICs in accordance with the international standards CLSI 2014 and EUCAST 2014. The highest levels of the MRSA resistance were stated against ciprofloxacin--92%(MIC50 32 mcg/ml), gentamicin--85% (MIC50 128 mcg/ml), erythromycin--54% (MIC50 32-mcg/ml) and clindainycin - 45% (MIC50 0...
2015: Antibiotiki i Khimioterapii︠a︡, Antibiotics and Chemoterapy [sic]
L Drago, E De Vecchi, L Cappelletti, C Vassena, M Toscano, M Bortolin, R Mattina, C L Romanò
Since the optimal incubation period of cultures for diagnosis of bone and joint infections is still a matter of debate, the present study aimed to evaluate the effects of different incubation periods (5 and 15 days) on microbial isolation. Samples from 387 patients with bone and joint infections (including prosthetic ones) were analyzed from March 2012 to February 2014. In 197 patients (51 %) growth was obtained within 48 hrs, while in 124 (32 %) and 66 (17 %) patients cultures yielded positive results within and after 5 days of incubation, respectively...
September 2015: European Journal of Clinical Microbiology & Infectious Diseases
Umang Jain, Craig A Midgen, Wilhelm J Schwaeble, Cordula M Stover, Andrew W Stadnyk
BACKGROUND: Interleukin 10-deficient mice (IL-10(-/-)) are a popular model used to dissect the mechanisms underlying inflammatory bowel diseases. The role of complement, a host defense mechanism that bridges the innate and adaptive immune systems, has not been described in this model. We therefore studied the effect of deficiency of properdin, a positive regulator of complement, on colitis in mice with the IL-10(-/-) background. METHODS: For acute colitis, IL-10(-/-) and IL-10/properdin double knockout (DKO) or radiation bone marrow-reconstituted chimeric mice, had piroxicam added to their powdered chow for 14 days...
July 2015: Inflammatory Bowel Diseases
Minjung You, Lijuan Liao, Soo Hyun Hong, Wanki Park, Dah In Kwon, Jeeyeon Lee, Minsoo Noh, Dong-Chan Oh, Ki-Bong Oh, Jongheon Shin
Terrelumamides A (1) and B (2), two new lumazine-containing peptides, were isolated from the culture broth of the marine-derived fungus Aspergillus terreus. From the results of combined spectroscopic and chemical analyses, the structures of these compounds were determined to be linear assemblies of 1-methyllumazine-6-carboxylic acid, an amino acid residue and anthranilic acid methyl ester connected by peptide bonds. These new compounds exhibited pharmacological activity by improving insulin sensitivity, which was evaluated in an adipogenesis model using human bone marrow mesenchymal stem cells...
March 2015: Marine Drugs
Mehdi Fatahi Bafghi, Parvin Heidarieh, Tahereh Soori, Sasan Saber, Alipasha Meysamie, Khavar Gheitoli, Shadi Habibnia, Masoumeh Rasouli Nasab, Seyyed Saeed Eshraghi
BACKGROUND: The genus Nocardia is a cause of infection in the lungs, skin, brain, cerebrospinal fluid, eyes, joints and kidneys. Nocardia isolation from polymicrobial specimens is difficult due to its slow growth. Several methods have been reported for Nocardia isolation from clinical samples. In the current study, we used three methods: paraffin baiting technique, paraffin agar, and conventional media for Nocardia isolation from various clinical specimens from Iranian patients. METHODS: In this study, we examined 517 samples from various clinical specimens such as: sputum of patients with suspected tuberculosis, bronchoalveolar lavage, sputum of patients with cystic fibrosis, tracheal aspirate, cutaneous and subcutaneous abscesses, cerebrospinal fluid, dental abscess, mycetoma, wound, bone marrow biopsy, and gastric lavage...
March 2015: Germs
Patricio Peñalba Arias, Ulrika Furustrand Tafin, Bertrand Bétrisey, Sebastian Vogt, Andrej Trampuz, Olivier Borens
Daptomycin is a promising candidate for local treatment of bone infection due to its activity against multi-resistant staphylococci. We investigated the activity of antibiotic-loaded PMMA against Staphylococcus epidermidis biofilms using an ultra-sensitive method bacterial heat detection method (microcalorimetry). PMMA cylinders loaded with daptomycin alone or in combination with gentamicin or PEG600, vancomycin and gentamicin were incubated with S. epidermidis-RP62A in tryptic soy broth (TSB) for 72 h. Cylinders were thereafter washed and transferred in microcalorimetry ampoules pre-filled with TSB...
February 2015: Injury
J P Blackmur, E Y H Tang, J Dave, A H R W Simpson
We compared the use of broth culture medium for samples taken in theatre with the standard practice of placing tissue samples in universal containers. A total of 67 consecutive patients had standard multiple samples of deep tissue harvested at surgery and distributed equally in theatre either to standard universal containers or to broth culture medium. These samples were cultured by direct and enrichment methods. The addition of broth in theatre to standard practice led to an increase in sensitivity from 83% to 95% and an increase in negative predictive value from 77% to 91%...
November 2014: Bone & Joint Journal
K Nazari Moghadam, H Aghili, A Rashed Mohassel, S Zahedpasha, A A Moghadamnia
AIM: The aim of this study was to compare the bacterial leakage of mineral trioxide aggregate (MTA), calcium enriched cement (CEM), and bone cement (BC) as repair materials in furcal perforations. METHODS: The pulp chambers of 57 human mandibular molar teeth were accessed and the root canal orifices were located. The roots were horizontally sectioned in the middle third. Composite resin was used to fill the root canal orifices and the apical end of the roots. The 1 mm furcation perforations were performed in the center of the pulp chamber floor, using diamond fissure burs...
June 2014: Minerva Stomatologica
Camila Rizek, Liang Fu, Leticia Cavalcanti Dos Santos, Gleice Leite, Jessica Ramos, Flavia Rossi, Thais Guimaraes, Anna S Levin, Silvia Figueiredo Costa
BACKGROUND: Carbapenemase genes are one of the most frequent mechanisms reported in carbapenem-resistant P. aeruginosa; however, description of P. aeruginosa co-harbouring two or more carbapenemases is unusual. METHODS: In this study we evaluated the presence of carbapenemase genes and the clonality of P. aeruginosa isolates obtained from a hospital over a 12-year period. A total of 127 isolates of carbapenem-resistant P. aeruginosa recovered from 109 patients feces (four samples), rectal swab (three samples), nasal swab (one sample) and anal abscess (one sample), were evaluated...
2014: Annals of Clinical Microbiology and Antimicrobials
M Clauss, U Furustrand Tafin, B Betrisey, N van Garderen, A Trampuz, T Ilchmann, M Bohner
Various compositions of synthetic calcium phosphates (CaP) have been proposed and their use has considerably increased over the past decades. Besides differences in physico-chemical properties, resorption and osseointegration, artificial CaP bone graft might differ in their resistance against biofilm formation. We investigated standardised cylinders of 5 different CaP bone grafts (cyclOS, chronOS (both β-TCP (tricalcium phosphate)), dicalcium phosphate (DCP), calcium-deficient hydroxyapatite (CDHA) and α-TCP)...
2014: European Cells & Materials
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