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copper metabolism

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https://www.readbyqxmd.com/read/29139577/copper-uptake-patterns-of-bioaccumulation-and-effects-in-glochidia-larvae-of-the-freshwater-mussel-lampsilis-cardium
#1
Marianna Basso Jorge, Adalto Bianchini, Chris M Wood, Patricia L Gillis
The early life stages of freshwater mussels are particularly sensitive to Cu contamination. We measured the acute toxicity, bioaccumulation and sublethal effects of Cu in glochidia. Additionally, we used radiolabeled Cu ((64) Cu) to examine the time-dependent kinetics of uptake over 24 h. (64) Cu uptake by live and dead glochidia exposed to 0.11 μmol/L exhibited similar hyperbolic patterns over the first 40 min, indicating an adsorptive phase independent of larval metabolism. Thereafter, uptake was linear with time, with a 10-fold lower bioaccumulation rate in live than in dead animals, representing a close to steady state of Cu regulation...
November 15, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29138237/natural-variation-in-ser1-and-ena6-underlie-condition-specific-growth-defects-in-saccharomyces%C3%A2-cerevisiae
#2
Amy Sirr, Adrian C Scott, Gareth A Cromie, Catherine L Ludlow, Vida Ahyong, Trey S Morgan, Teresa Gilbert, Aimée M Dudley
Despite their ubiquitous use in laboratory strains, naturally occurring loss-of-function mutations in genes encoding core metabolic enzymes are relatively rare in wild isolates of Saccharomyces cerevisiae Here, we identify a naturally occurring serine auxotrophy in a sake brewing strain from Japan. Through a cross with a honey wine (white tecc) brewing strain from Ethiopia, we map the minimal medium growth defect to SER1, which encodes 3-phosphoserine aminotransferase and is orthologous to the human disease gene, PSAT1 To investigate the impact of this polymorphism under conditions of abundant external nutrients, we examine growth in rich medium alone or with additional stresses, including the drugs caffeine and rapamycin and relatively high concentrations of copper, salt, and ethanol...
November 14, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29131568/diisonitrile-natural-product-sf2768-functions-as-a-chalkophore-that-mediates-copper-acquisition-in-streptomyces-thioluteus
#3
Lijuan Wang, Mengyi Zhu, Qingbo Zhang, Xu Zhang, Panlei Yang, Zihui Liu, Yun Deng, Yiguang Zhu, Xueshi Huang, Li Han, Shengqing Li, Jing He
A non-ribosomal peptide synthetase (NRPS) gene cluster (sfa) was identified in Streptomyces thioluteus to direct the biosynthesis of the diisonitrile antibiotic SF2768. Its biosynthetic pathway was reasonably proposed based on bioinformatics analysis, metabolic profiles of mutants and the elucidation of the intermediate and shunt product structures. Bioinformatics-based alignment found a putative ATP-binding cassette (ABC) transporter related to iron import within the biosynthetic gene cluster, which implied that the product might be a siderophore...
November 13, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/29122590/physiological-serum-copper-concentrations-found-in-malignancies-cause-unfolding-induced-aggregation-of-human-serum-albumin-in-vitro
#4
Asim Rizvi, Mohd Furkan, Imrana Naseem
Malignancies are characterized by several drastic metabolic changes, one of which is a progressive rise in the levels of serum copper. This rise in serum copper is documented across all malignancies and across malignancies in several species. This study aims to explore in vitro the effect of increased copper levels on the structure of the blood protein human serum albumin. Exposure of human serum albumin to physiologically relevant copper concentrations for 21 days resulted in structural modifications in the protein which were evident by changes in the intrinsic florescence...
November 6, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29121854/monitoring-interactions-inside-cells-by-advanced-spectroscopies-overview-of-copper-transporters-and-cisplatin
#5
Alessia Lasorsa, Giovanni Natile, Antonio Rosato, Francesco Tadini-Buoninsegni, Fabio Arnesano
BACKGROUND: Resistance, either at the onset of the treatment or developed after an initial positive response, is a major limitation of antitumor therapy. In the case of platinum-based drugs, copper transporters have been found to interfere with drug trafficking by facilitating the import or favoring the platinum export and inactivation. METHODS: The use of powerful spectroscopic, spectrometric and computational methods have allowed a deep structural insight into the mode of interaction of platinum drugs with the metal-binding domains of the transporter proteins...
November 10, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29114657/chemistry-of-mammalian-metallothioneins-and-their-interaction-with-amyloidogenic-peptides-and-proteins
#6
Elena Atrián-Blasco, Alice Santoro, Dean L Pountney, Gabriele Meloni, Christelle Hureau, Peter Faller
Cu and Zn ions are essential in most living beings. Their metabolism is critical for health and mis-metabolism can be lethal. In the last two decades, a large body of evidence has reported the role of copper, zinc and iron, and oxidative stress in several neurodegenerative diseases like Alzheimer's, Parkinson's, prion diseases, etc. To what extent this mis-metabolism is causative or a consequence of these diseases is still a matter of research. In this context metallothioneins (MTs) appear to play a central gate-keeper role in controlling aberrant metal-protein interactions...
November 8, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/29114014/arabidopsis-pollen-fertility-requires-the-transcription-factors-cit1-and-spl7-that-regulate-copper-delivery-to-anthers-and-jasmonic-acid-synthesis
#7
Jiapei Yan, Ju-Chen Chia, Huajin Sheng, Ha-Il Jung, Tetiana-Olena Zavodna, Zhang Lu, Rong Huang, Chen Jiao, Eric Craft, Zhangjun Fei, Leon Vincent Kochian, Olena K Vatamaniuk
A deficiency of the micronutrient copper (Cu) leads to infertility and grain/seed yield reduction in plants. How Cu affects fertility, which reproductive structures require Cu, and which transcriptional networks coordinate Cu delivery to reproductive organs is poorly understood. Using RNA-seq analysis, we showed that the expression of a gene encoding a novel transcription factor, CITF1 (Cu-deficiency Induced Transcription Factor 1), was strongly upregulated in Arabidopsis thaliana flowers subjected to Cu deficiency...
November 7, 2017: Plant Cell
https://www.readbyqxmd.com/read/29100100/link-of-impaired-metal-ion-homeostasis-to-mitochondrial-dysfunction-in-neurons
#8
REVIEW
Eunju Nam, Jiyeon Han, Jong-Min Suh, Yelim Yi, Mi Hee Lim
Manganese, iron, copper, and zinc are observed to play essential roles in mitochondria. The overload and depletion of metal ions in mitochondria under pathological conditions, however, could disturb mitochondrial compartments and functions leading to cell death. In this review, we mainly summarize how impaired metal ion homeostasis affects mitochondrial systems, such as membrane potentials, the tricarboxylic acid cycle, oxidative phosphorylation, and glutathione metabolism. In addition, based on current findings, we briefly describe a recent understanding of the relationship among metal ion dysregulation, mitochondrial dysfunction, and the pathogeneses of neurodegenerative diseases...
October 27, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29099786/the-extracellular-domain-of-human-high-affinity-copper-transporter-hndctr1-synthesized-by-e-coli-cells-chelates-silver-and-copper-ions-in-vivo
#9
Tatiana P Sankova, Iurii A Orlov, Andrey N Saveliev, Demid A Kirilenko, Polina S Babich, Pavel N Brunkov, Ludmila V Puchkova
There is much interest in effective copper chelators to correct copper dyshomeostasis in neurodegenerative and oncological diseases. In this study, a recombinant fusion protein for expression in Escherichia coli cells was constructed from glutathione-S-transferase (GST) and the N-terminal domain (ectodomain) of human high affinity copper transporter CTR1 (hNdCTR1), which has three metal-bound motifs. Several biological properties of the GST-hNdCTR1 fusion protein were assessed. It was demonstrated that in cells, the protein was prone to oligomerization, formed inclusion bodies and displayed no toxicity...
November 3, 2017: Biomolecules
https://www.readbyqxmd.com/read/29098318/-wilson-s-disease-what-has-been-confirmed-in-diagnostic-and-therapy
#10
REVIEW
E-D Pfister
Wilson's disease (WD) is a rare autosomal recessive disorder characterized by abnormal copper accumulation. Presenting a broad variety of phenotypes and, thus, being a chameleon within the group of metabolic diseases, the manifold clinical symptoms of WD can include hepatologic, neurologic, and psychiatric manifestations. Early onset presentations in infancy and late-onset manifestations in adults older than 70 years of age have been described. If the typical laboratory blood test values are missing, the diagnosis of WD may be difficult and often involves a combination of different parameters...
November 2, 2017: Der Internist
https://www.readbyqxmd.com/read/29080170/analysis-of-the-intracellular-localization-of-transiently-expressed-and-fluorescently-labeled-copper-containing-amine-oxidases-diamine-oxidase-and-n-methylputrescine-oxidase-in-tobacco-using-an-agrobacterium-infiltration-protocol
#11
Tsubasa Shoji
The intracellular localization of enzymes provides key information for understanding complex metabolic pathways. Based on enzyme localization data, the involvement of multiple organelles and the movement of metabolites between cellular compartments have been suggested for a number of pathways. Transient expression of fluorescently tagged proteins in the leaves of Nicotiana benthamiana through Agrobacterium infiltration is a simple and versatile way to examine the intracellular localization of proteins of interest...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29073182/blood-parameters-as-biomarkers-in-a-salmonella-spp-disease-model-of-weaning-piglets
#12
Emili Barba-Vidal, Victor Fernando Buttow Roll, Edgar Garcia Manzanilla, Carlos Torrente, José Antonio Moreno Muñoz, José Francisco Pérez, Susana María Martín-Orúe
BACKGROUND: The weaning pig is used as an experimental model to assess the impact of diet on intestinal health. Blood parameters (BP) are considered a useful tool in humans, but there is very scarce information of such indicators in the weaning pig. The objective of the present study is to evaluate the use of different BP as indicators in an experimental model of salmonellosis. METHODOLOGY: Seventy-two 28-day-old piglets were divided into four groups in a 2x2 factorial arrangement, with animals receiving or not a probiotic combination based on B...
2017: PloS One
https://www.readbyqxmd.com/read/29070548/excess-iron-considerations-related-to-development-and-early-growth
#13
REVIEW
Marianne Wessling-Resnick
What effects might arise from early life exposures to high iron? This review considers the specific effects of high iron on the brain, stem cells, and the process of erythropoiesis and identifies gaps in our knowledge of what molecular damage may be incurred by oxidative stress that is imparted by high iron status in early life. Specific areas to enhance research on this topic include the following: longitudinal behavioral studies of children to test associations between iron exposures and mood, emotion, cognition, and memory; animal studies to determine epigenetic changes that reprogram brain development and metabolic changes in early life that could be followed through the life course; and the establishment of human epigenetic markers of iron exposures and oxidative stress that could be monitored for early origins of adult chronic diseases...
October 25, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29063292/the-six-metal-binding-domains-in-human-copper-transporter-atp7b-molecular-biophysics-and-disease-causing-mutations
#14
REVIEW
Candan Ariöz, Yaozong Li, Pernilla Wittung-Stafshede
Wilson Disease (WD) is a hereditary genetic disorder, which coincides with a dysfunctional copper (Cu) metabolism caused by mutations in ATP7B, a membrane-bound P1B-type ATPase responsible for Cu export from hepatic cells. The N-terminal part (~ 600 residues) of the multi-domain 1400-residue ATP7B constitutes six metal binding domains (MBDs), each of which can bind a copper ion, interact with other ATP7B domains as well as with different proteins. Although the ATP7B's MBDs have been investigated in vitro and in vivo intensively, it remains unclear how these domains modulate overall structure, dynamics, stability and function of ATP7B...
October 23, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
https://www.readbyqxmd.com/read/29062176/supplementation-of-%C3%AE-tocopherol-attenuates-minerals-disturbance-oxidative-stress-and-apoptosis-occurring-in-favism
#15
Khaled M M Koriem, Mahmoud S Arbid, Nawal E Gomaa
The favism is a metabolic disease that characterized with an acute hemolytic anemia where α-tocopherol is a type of tocopherol accumulated inside the human body. The objective of such a study was established to evaluate the effect of α-tocopherol in favism disorders. A total of 75 human cases were divided into 5 groups as follow; group 1 normal cases without any treatment and group 2 normal cases orally administrated α-tocopherol (200 mg/kg) once a day over 30 days period. Group 3 favism patients without any treatment...
October 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/29058724/a-crispr-screen-identifies-a-pathway-required-for-paraquat-induced-cell-death
#16
Colleen R Reczek, Kıvanç Birsoy, Hyewon Kong, Inmaculada Martínez-Reyes, Tim Wang, Peng Gao, David M Sabatini, Navdeep S Chandel
Paraquat, a herbicide linked to Parkinson's disease, generates reactive oxygen species (ROS), which causes cell death. Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat-induced cell death. Furthermore, our results revealed POR as the source of paraquat-induced ROS production...
October 23, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/29051457/hydrophobic-sand-is-a-non-toxic-method-of-urine-collection-appropriate-for-urinary-metal-analysis-in-the-rat
#17
Jessica F Hoffman, Vernieda B Vergara, Steven R Mog, John F Kalinich
Hydrophobic sand is a relatively new method of urine collection in the rodent, comparable to the established method using a metabolic cage. Urine samples are often used in rodent research, especially for biomarkers of health changes after internal contamination from embedded metals, such as in a model of a military shrapnel wound. However, little research has been done on the potential interference of hydrophobic sand with urine metal concentrations either by contamination from the sand particulate, or adsorption of metals from the urine...
October 11, 2017: Toxics
https://www.readbyqxmd.com/read/29050588/sequential-one-pot-multienzyme-synthesis-of-hyaluronan-and-its-derivative
#18
Shuang Li, Shuaishuai Wang, Xuan Fu, Xian-Wei Liu, Peng George Wang, Junqiang Fang
Hyaluronan (HA) is a linear polysaccharide composed of repeating disaccharide units. It has been well documented to play an array of biological functions in cancer events. Here, we reported a sequential one-pot multienzyme (OPME) strategy for in vitro synthesis of HA and its derivatives. The strategy, which combined in situ sugar nucleotides generation with HA chain polymerization, could convert cheap monosaccharides into HA polymers without consuming exogenous sugar nucleotide donors. HA polymers (number-average molecular weight ranged from 1...
December 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29048718/azido-functionalized-5-cap-analogs-for-preparation-of-translationally-active-mrnas-suitable-for-fluorescent-labeling-in-living-cells
#19
Adam Mamot, Pawel Sikorski, Marcin Warminski, Joanna Kowalska, Jacek Jemielity
The 7-methylguanosine (m7G) cap structure is a unique feature present at the 5' ends of messenger RNAs (mRNAs) that can be subjected to extensive modifications, resulting in alterations to mRNA properties (e.g. translability, susceptibility to degradation). It also can provide molecular tools to study mRNA metabolism. Here, we developed new mRNA 5' cap-based tools that enable the site-specific labeling of RNA at the 5' end using strain-promoted azide-alkyne cycloaddition (SPAAC), i.e. bioorthogonal, copper-free click chemistry, and support the mRNA's basic function in protein biosynthesis...
October 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29040823/urea-increased-nickel-and-copper-accumulation-in-the-leaves-of-egeria-densa-planch-casp-and-ceratophyllum-demersum-l-during-short-term-exposure
#20
Maria Maleva, Galina Borisova, Nadezhda Chukina, Adarsh Kumar
In the present study, two fresh water plant species Egeria densa (Planch.) Casp. and Ceratophyllum demersum L. were subjected to separate and combined action of urea (2mМ) and metals (Ni and Cu, 10μM) to investigate the phytoremediation potential of these two submerged macrophytes during short-term experiments (48h). Both submerged macrophytes demonstrated high accumulative potential for Ni and Cu (average bioconcentration factors were 2505 for Ni and 3778 for Cu). The urea (2 mM) was not significantly toxic for studied plant species...
October 14, 2017: Ecotoxicology and Environmental Safety
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