keyword
MENU ▼
Read by QxMD icon Read
search

Kinesin-1

keyword
https://www.readbyqxmd.com/read/29790963/the-presenilin-loop-region-is-essential-for-glycogen-synthase-kinase-3-beta-mediated-functions-on-motor-proteins-during-axonal-transport
#1
Rupkatha Banerjee, Zoe Rudloff, Crystal Naylor, Michael Yu, Shermali Gunawardena
Neurons require intracellular transport of essential components for function and viability, and defects in transport has been implicated in many neurodegenerative diseases including Alzheimer's disease (AD). One possible mechanism by which transport defects could occur is by improper regulation of molecular motors. Previous work showed that reduction of Presenilin (PS) or Glycogen synthase kinase 3 beta (GSK3β) stimulated APP vesicle motility. Excess GSK3β caused transport defects and increased motor binding to membranes, while reduction of PS decreased active GSK3β and motor binding to membranes...
May 22, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29790183/fasting-inhibits-the-recruitment-of-kinesin-1-to-lipid-droplets-and-stalls-hepatic-triglyceride-secretion
#2
Ryan J Schulze, Mark A McNiven
No abstract text is available yet for this article.
May 23, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29782552/role-of-kinesins-in-directed-adenovirus-transport-and-cytoplasmic-exploration
#3
Jie Zhou, Julian Scherer, Julie Yi, Richard B Vallee
Many viruses, including adenovirus, exhibit bidirectional transport along microtubules following cell entry. Cytoplasmic dynein is responsible for microtubule minus end transport of adenovirus capsids after endosomal escape. However, the identity and roles of the opposing plus end-directed motor(s) remain unknown. We performed an RNAi screen of 38 kinesins, which implicated Kif5B (kinesin-1 family) and additional minor kinesins in adenovirus 5 (Ad5) capsid translocation. Kif5B RNAi markedly increased centrosome accumulation of incoming Ad5 capsids in human A549 pulmonary epithelial cells within the first 30 min post infection, an effect dramatically enhanced by blocking Ad5 nuclear pore targeting using leptomycin B...
May 21, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29728423/autoinhibition-of-kinesin-1-is-essential-to-the-dendrite-specific-localization-of-golgi-outposts
#4
Michael T Kelliher, Yang Yue, Ashley Ng, Daichi Kamiyama, Bo Huang, Kristen J Verhey, Jill Wildonger
Neuronal polarity relies on the selective localization of cargo to axons or dendrites. The molecular motor kinesin-1 moves cargo into axons but is also active in dendrites. This raises the question of how kinesin-1 activity is regulated to maintain the compartment-specific localization of cargo. Our in vivo structure-function analysis of endogenous Drosophila melanogaster kinesin-1 reveals a novel role for autoinhibition in enabling the dendrite-specific localization of Golgi outposts. Mutations that disrupt kinesin-1 autoinhibition result in the axonal mislocalization of Golgi outposts...
May 4, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29712642/spatial-regulation-of-microtubule-disruption-during-dendrite-pruning-in-drosophila
#5
Svende Herzmann, Ina Götzelmann, Lea-Franziska Reekers, Sebastian Rumpf
Large scale neurite pruning is an important specificity mechanism during neuronal morphogenesis. Drosophila sensory neurons prune their larval dendrites during metamorphosis. Pruning dendrites are severed in their proximal regions, but how this spatial information is encoded is not clear. Dendrite severing is preceded by local breakdown of dendritic microtubules through PAR-1-mediated inhibition of Tau. Here, we investigated spatial aspects of microtubule breakdown during dendrite pruning. Live imaging of fluorescently tagged tubulin shows that microtubule breakdown first occurs at proximal dendritic branchpoints, followed by breakdown at more distal branchpoints, suggesting that the process is triggered by a signal emanating from the soma...
April 30, 2018: Development
https://www.readbyqxmd.com/read/29662074/competition-between-microtubule-associated-proteins-directs-motor-transport
#6
Brigette Y Monroy, Danielle L Sawyer, Bryce E Ackermann, Melissa M Borden, Tracy C Tan, Kassandra M Ori-McKenney
Within cells, motor and non-motor microtubule-associated proteins (MAPs) simultaneously converge on the microtubule. How the binding activities of non-motor MAPs are coordinated and how they contribute to the balance and distribution of motor transport is unknown. Here, we examine the relationship between MAP7 and tau owing to their antagonistic roles in vivo. We find that MAP7 and tau compete for binding to microtubules, and determine a mechanism by which MAP7 displaces tau from the lattice. MAP7 promotes kinesin-based transport in vivo and strongly recruits kinesin-1 to the microtubule in vitro, providing evidence for direct enhancement of motor motility by a MAP...
April 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29606421/a-camp-pka-kinesin-1-axis-promotes-the-axonal-transport-of-mitochondria-in-aging-drosophila-neurons
#7
Alessio Vagnoni, Simon L Bullock
Mitochondria play fundamental roles within cells, including energy provision, calcium homeostasis, and the regulation of apoptosis. The transport of mitochondria by microtubule-based motors is critical for neuronal structure and function. This process allows local requirements for mitochondrial functions to be met and also facilitates recycling of these organelles [1, 2]. An age-related reduction in mitochondrial transport has been observed in neurons of mammalian and non-mammalian organisms [3-6], and has been proposed to contribute to the broader decline in neuronal function that occurs during aging [3, 5-7]...
March 20, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29604873/a-fluid-membrane-enhances-the-velocity-of-cargo-transport-by-small-teams-of-kinesin-1
#8
Qiaochu Li, Kuo-Fu Tseng, Stephen J King, Weihong Qiu, Jing Xu
Kinesin-1 (hereafter referred to as kinesin) is a major microtubule-based motor protein for plus-end-directed intracellular transport in live cells. While the single-molecule functions of kinesin are well characterized, the physiologically relevant transport of membranous cargos by small teams of kinesins remains poorly understood. A key experimental challenge remains in the quantitative control of the number of motors driving transport. Here we utilized "motile fraction" to overcome this challenge and experimentally accessed transport by a single kinesin through the physiologically relevant transport by a small team of kinesins...
March 28, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29572128/a-simple-and-reproducible-protocol-of-glass-surface-silanization-for-tirf-microscopy-imaging
#9
Michał Szkop, Beata Kliszcz, Andrzej A Kasprzak
We describe a simple and reproducible protocol for the preparation of microscope glass slides for in vitro motility assays that use total internal reflection fluorescence microscopy. The developed method utilizes trimethylchlorosilane (TMCS) as a silanizing reagent, which in the presence of imidazole as a catalyst and under optimized conditions enables reproducible preparation of high-quality hydrophobic glass surfaces. This method presents a simplification and improvement in reproducibility over the commonly applied protocol utilizing dichlorodimethylsilane (DDS) as a silanizing agent...
March 20, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29531331/kinesin-expands-and-stabilizes-the-gdp-microtubule-lattice
#10
Daniel R Peet, Nigel J Burroughs, Robert A Cross
Kinesin-1 is a nanoscale molecular motor that walks towards the fast-growing (plus) ends of microtubules, hauling molecular cargo to specific reaction sites in cells. Kinesin-driven transport is central to the self-organization of eukaryotic cells and shows great promise as a tool for nano-engineering 1 . Recent work hints that kinesin may also play a role in modulating the stability of its microtubule track, both in vitro2,3 and in vivo 4 , but the results are conflicting5-7 and the mechanisms are unclear...
May 2018: Nature Nanotechnology
https://www.readbyqxmd.com/read/29519941/kinesin-1-is-a-new-actor-involved-in-platelet-secretion-and-thrombus-stability
#11
Frédéric Adam, Alexandre Kauskot, Mathieu Kurowska, Nicolas Goudin, Isabelle Munoz, Jean-Claude Bordet, Jian-Dong Huang, Marijke Bryckaert, Alain Fischer, Delphine Borgel, Geneviève de Saint Basile, Olivier D Christophe, Gaël Ménasché
OBJECTIVE: Platelet secretion is crucial for many physiological platelet responses. Even though several regulators of the fusion machinery for secretory granule exocytosis have been identified in platelets, the underlying mechanisms are not yet fully characterized. APPROACH AND RESULTS: By studying a mouse model (cKO [conditional knockout]Kif5b ) lacking Kif5b (kinesin-1 heavy chain) in its megakaryocytes and platelets, we evidenced unstable hemostasis characterized by an increase of blood loss associated to a marked tendency to rebleed in a tail-clip assay and thrombus instability in an in vivo thrombosis model...
May 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29519888/nesprins-and-opposing-microtubule-motors-generate-a-point-force-that-drives-directional-nuclear-motion-in-migrating-neurons
#12
You Kure Wu, Hiroki Umeshima, Junko Kurisu, Mineko Kengaku
Nuclear migration of newly born neurons is essential for cortex formation in the brain. The nucleus is translocated by actin and microtubules, yet the actual force generated by the interplay of these cytoskeletons remains elusive. High-resolution time-lapse observation of migrating murine cerebellar granule cells revealed that the nucleus actively rotates along the direction of its translocation, independently of centrosome motion. Pharmacological and molecular perturbation indicated that spin torque is primarily generated by microtubule motors through the LINC complex in the absence of actomyosin contractility...
March 8, 2018: Development
https://www.readbyqxmd.com/read/29510577/loss-of-actin-based-motility-impairs-ectromelia-virus-release-in-vitro-but-is-not-critical-to-spread-in-vivo
#13
Melanie Laura Duncan, Jacquelyn Horsington, Preethi Eldi, Zahrah Al Rumaih, Gunasegaran Karupiah, Timothy P Newsome
Ectromelia virus (ECTV) is an orthopoxvirus and the causative agent of mousepox. Like other poxviruses such as variola virus (agent of smallpox), monkeypox virus and vaccinia virus (the live vaccine for smallpox), ECTV promotes actin-nucleation at the surface of infected cells during virus release. Homologs of the viral protein A36 mediate this function through phosphorylation of one or two tyrosine residues that ultimately recruit the cellular Arp2/3 actin-nucleating complex. A36 also functions in the intracellular trafficking of virus mediated by kinesin-1...
March 5, 2018: Viruses
https://www.readbyqxmd.com/read/29507520/how-the-formation-of-amyloid-plaques-and-neurofibrillary-tangles-may-be-related-a-mathematical-modelling-study
#14
I A Kuznetsov, A V Kuznetsov
We develop a mathematical model that enables us to investigate possible mechanisms by which two primary markers of Alzheimer's disease (AD), extracellular amyloid plaques and intracellular tangles, may be related. Our model investigates the possibility that the decay of anterograde axonal transport of amyloid precursor protein (APP), caused by toxic tau aggregates, leads to decreased APP transport towards the synapse and APP accumulation in the soma. The developed model thus couples three processes: (i) slow axonal transport of tau, (ii) tau misfolding and agglomeration, which we simulated by using the Finke-Watzky model and (iii) fast axonal transport of APP...
February 2018: Proceedings. Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/29445114/kinesin-1-regulates-cilia-length-through-an-interaction-with-the-bardet-biedl-syndrome-related-protein-ccdc28b
#15
Rossina Novas, Magdalena Cardenas-Rodriguez, Paola Lepanto, Matías Fabregat, Magela Rodao, María Inés Fariello, Mauricio Ramos, Camila Davison, Gabriela Casanova, Lucía Alfaya, Federico Lecumberry, Gualberto González-Sapienza, Florencia Irigoín, Jose L Badano
Bardet-Biedl syndrome (BBS) is a ciliopathy characterized by retinal degeneration, obesity, polydactyly, renal disease and mental retardation. CCDC28B is a BBS-associated protein that we have previously shown plays a role in cilia length regulation whereby its depletion results in shortened cilia both in cells and Danio rerio (zebrafish). At least part of that role is achieved by its interaction with the mTORC2 component SIN1, but the mechanistic details of this interaction and/or additional functions that CCDC28B might play in the context of cilia remain poorly understood...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29432173/a-posttranslational-modification-of-the-mitotic-kinesin-eg5-that-enhances-its-mechanochemical-coupling-and-alters-its-mitotic-function
#16
Joseph M Muretta, Babu J N Reddy, Guido Scarabelli, Alex F Thompson, Shashank Jariwala, Jennifer Major, Monica Venere, Jeremy N Rich, Belinda Willard, David D Thomas, Jason Stumpff, Barry J Grant, Steven P Gross, Steven S Rosenfeld
Numerous posttranslational modifications have been described in kinesins, but their consequences on motor mechanics are largely unknown. We investigated one of these-acetylation of lysine 146 in Eg5-by creating an acetylation mimetic lysine to glutamine substitution (K146Q). Lysine 146 is located in the α2 helix of the motor domain, where it makes an ionic bond with aspartate 91 on the neighboring α1 helix. Molecular dynamics simulations predict that disrupting this bond enhances catalytic site-neck linker coupling...
February 20, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29401437/motor-reattachment-kinetics-play-a-dominant-role-in-multimotor-driven-cargo-transport
#17
Qingzhou Feng, Keith J Mickolajczyk, Geng-Yuan Chen, William O Hancock
Kinesin-based cargo transport in cells frequently involves the coordinated activity of multiple motors, including kinesins from different families that move at different speeds. However, compared to the progress at the single-molecule level, mechanisms by which multiple kinesins coordinate their activity during cargo transport are poorly understood. To understand these multimotor coordination mechanisms, defined pairs of kinesin-1 and kinesin-2 motors were assembled on DNA scaffolds and their motility examined in vitro...
January 23, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29387789/superresolution-and-pulse-chase-imaging-reveal-the-role-of-vesicle-transport-in-polar-growth-of-fungal-cells
#18
Lu Zhou, Minoas Evangelinos, Valentin Wernet, Antonia F Eckert, Yuji Ishitsuka, Reinhard Fischer, G Ulrich Nienhaus, Norio Takeshita
Polarized growth of filamentous fungi requires continuous transport of biomolecules to the hyphal tip. To this end, construction materials are packaged in vesicles and transported by motor proteins along microtubules and actin filaments. We have studied these processes with quantitative superresolution localization microscopy of live Aspergillus nidulans cells expressing the photoconvertible protein mEosFP thermo fused to the chitin synthase ChsB. ChsB is mainly located at the Spitzenkörper near the hyphal tip and produces chitin, a key component of the cell wall...
January 2018: Science Advances
https://www.readbyqxmd.com/read/29382807/correction-for-phosphorylation-of-multiple-sites-within-an-acidic-region-of-alcadein-%C3%AE-is-required-for-kinesin-1-association-and-golgi-exit-of-alcadein-%C3%AE-cargo
#19
(no author information available yet)
No abstract text is available yet for this article.
February 1, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29380607/measuring-microtubule-supertwist-and-defects-by-three-dimensional-force-clamp-tracking-of-single-kinesin-1-motors
#20
Michael Bugiel, Aniruddha Mitra, Salvatore Girardo, Stefan Diez, Erik Schäffer
Three-dimensional (3D) nanometer tracking of single biomolecules provides important information about their biological function. However, existing microscopy approaches often have only limited spatial or temporal precision and do not allow the application of defined loads. Here, we developed and applied a high-precision 3D-optical-tweezers force clamp to track in vitro the 3D motion of single kinesin-1 motor proteins along microtubules. To provide the motors with unimpeded access to the whole microtubule lattice, we mounted the microtubules on topographic surface features generated by UV-nanoimprint lithography...
February 14, 2018: Nano Letters
keyword
keyword
47212
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"