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Nanoparticles and molecular imaging

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https://www.readbyqxmd.com/read/28641018/three-dimensional-imaging-of-transparent-tissues-via-metal-nanoparticle-labeling
#1
Abdullah Muhammad Syed, Shrey Sindhwani, Stefan Wilhelm, Benjamin R Kingston, Dennis S W Lee, Jennifer L Gommerman, Warren C W Chan
Chemical probes are key components of the bioimaging toolbox as they label biomolecules in cells and tissues. The new challenge in bioimaging is to design chemical probes for 3-Dimensional (3D) tissue imaging. Here, we discovered that the scattering of metal nanoparticles can provide imaging contrast in intact tissues in 3D. The nanoparticles can act as a template for chemically growing a metal layer to further enhance the scattering signal. The use of chemically-grown nanoparticles in whole tissues can amplify the scattering to produce a 1...
June 22, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28640995/colloidal-gold-nanoparticles-induce-changes-in-cellular-and-subcellular-morphology
#2
Xiaowei Ma, Raimo Hartmann, Dorleta Jimenez de Aberasturi, Fang Yang, Stefaan J H Soenen, Bella B Manshian, Jonas Franz, Daniel Valdeperez, Beatriz Pelaz, Neus Feliu, Norbert Hampp, Christoph Riethmüller, Henning Vieker, Natalie Frese, Armin Gölzhäuser, Michael Simonich, Robert L Tanguay, Xing-Jie Liang, Wolfgang J Parak
Exposure of cells to colloidal nanoparticles (NPs) can have concentration-dependent harmful effects. Mostly, such effects are monitored with biochemical assays or probes from molecular biology, i.e. viability assays, gene expression profiles, etc., neglecting that the presence of NPs can also drastically affect cellular morphology. In the case of polymer-coated Au NPs we demonstrate that upon NP internalization, cells undergo lysosomal swelling, alterations in mitochondrial morphology, disturbances in actin and tubulin cytoskeleton and associated signaling, and reduction of focal adhesion contact area and number of filopodia...
June 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28639669/plasmon-enhanced-fluorescence-spectroscopy
#3
REVIEW
Jian-Feng Li, Chao-Yu Li, Ricardo F Aroca
Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters...
June 22, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28638783/recent-advances-of-activatable-molecular-probes-based-on-semiconducting-polymer-nanoparticles-in-sensing-and-imaging
#4
REVIEW
Yan Lyu, Kanyi Pu
Molecular probes that change their signals in response to the target of interest have a critical role in fundamental biology and medicine. Semiconducting polymer nanoparticles (SPNs) have recently emerged as a new generation of purely organic photonic nanoagents with desirable properties for biological applications. In particular, tunable optical properties of SPNs allow them to be developed into photoluminescence, chemiluminescence, and photoacoustic probes, wherein SPNs usually serve as the energy donor and internal reference for luminescence and photoacoustic probes, respectively...
June 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/28626876/optical-molecular-imaging-guided-radiation-therapy-part-2-integrated-x-ray-and-fluorescence-molecular-tomography
#5
Junwei Shi, Thirupandiyur S Udayakumar, Zhiqun Wang, Nesrin Dogan, Alan Pollack, Yidong Yang
PURPOSE: Differentiating tumor from its surrounding soft tissues is challenging for x-ray computed tomography (CT). Fluorescence molecular tomography (FMT) can directly localize the internal tumors targeted with specific fluorescent probes. A FMT system was developed and integrated onto a CT guided irradiator to improve tumor localization for image guided radiation. METHODS: The FMT system was aligned orthogonal to the cone beam CT onboard our previously developed image guided small animal arc radiation treatment system (iSMAART)...
June 19, 2017: Medical Physics
https://www.readbyqxmd.com/read/28623338/high-resolution-imaging-of-a-cell-attached-nanointerface-using-a-gold-nanoparticle-two-dimensional-sheet
#6
Shihomi Masuda, Yuhki Yanase, Eiji Usukura, Sou Ryuzaki, Pangpang Wang, Koichi Okamoto, Thasaneeya Kuboki, Satoru Kidoaki, Kaoru Tamada
This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28615226/raman-encoded-molecular-imaging-remi-with-topically-applied-sers-nanoparticles-for-intraoperative-guidance-of-lumpectomy
#7
Yu Wang, Nicholas P Reder, Soyoung Kang, Adam K Glaser, Qian Yang, Matthew A Wall, Sara H Javid, Suzanne Dintzis, Jonathan T C Liu
Intraoperative identification of carcinoma at lumpectomy margins would enable reduced re-excision rates, which are currently as high as 20-50%. While imaging of disease-associated biomarkers can identify malignancies with high specificity, multiplexed imaging of such biomarkers is necessary to detect molecularly heterogeneous carcinomas with high sensitivity. We have developed a Raman-encoded molecular imaging (REMI) technique in which targeted nanoparticles are topically applied on excised tissues to enable rapid visualization of a multiplexed panel of cell surface biomarkers at surgical margin surfaces...
June 14, 2017: Cancer Research
https://www.readbyqxmd.com/read/28614623/a-carbon-dot-based-fluorescent-nanosensor-for-simple-visualization-of-bacterial-nucleic-acids
#8
Ha Neul Lee, Jea-Sung Ryu, Cheong Shin, Hyun Jung Chung
A simple and facile method for sensing of nucleic acids is in great need for disease biomarker detection and diagnosis. Herein, a fluorescent nanosensor utilizing carbon dot nanoparticles is introduced that form visible precipitates in the presence of target DNA. Carbon dot nanoparticles are fabricated by microwave pyrolysis of polyethylenimine, which emits strong photoluminescence and can form precipitates when added to target DNA oligonucleotides. The precipitates can be easily visualized by UV illumination, and data can be acquired as images using a smartphone, which are analyzed for quantification...
June 14, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28612952/a-nanoparticle-decorated-biomolecule-responsive-polymer-enables-robust-signaling-cascade-for-biosensing
#9
Zuan-Tao Lin, Jianhua Gu, Chien-Hung Li, T Randall Lee, Lixin Xie, Shuo Chen, Piao-Yang Cao, Shan Jiang, Yulin Yuan, Xia Hong, Hongting Wang, Dezhi Wang, Xifan Wang, Gang-Biao Jiang, Mikala Heon, Tianfu Wu
To meet the increasing demands for ultrasensitivity in monitoring trace amounts of low-abundance early biomarkers or environmental toxins, the development of a robust sensing system is urgently needed. Here, a novel signal cascade strategy is reported via an ultrasensitive polymeric sensing system (UPSS) composed of gold nanoparticle (gNP)-decorated polymer, which enables gNP aggregation in polymeric network and electrical conductance change upon specific aptamer-based biomolecular recognition. Ultralow concentrations of thrombin (10(-18) m) as well as a low molecular weight anatoxin (165 Da, 10(-14) m) are detected selectively and reproducibly...
June 14, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28612465/mass-spectrometry-imaging-of-low-molecular-weight-compounds-in-garlic-allium-sativum-l-with-gold-nanoparticle-enhanced-target
#10
Maria Misiorek, Justyna Sekuła, Tomasz Ruman
INTRODUCTION: Garlic (Allium sativum) is the subject of many studies due to its numerous beneficial properties. Although compounds of garlic have been studied by various analytical methods, their tissue distributions are still unclear. Mass spectrometry imaging (MSI) appears to be a very powerful tool for the identification of the localisation of compounds within a garlic clove. OBJECTIVE: Visualisation of the spatial distribution of garlic low-molecular weight compounds with nanoparticle-based MSI...
June 13, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28609289/adverse-eff-ects-of-polymeric-nanoparticle-poly-ethylene-glycol-block-polylactide-methyl-ether-peg-b-pla-on-steroid-hormone-secretion-by-porcine-granulosa-cells
#11
Sona Scsukova, Mlynarcikova A Bujnakova, A Kiss, E Rollerova
OBJECTIVES: Development of nanoparticles (NPs) for biomedical applications, including medical imaging and drug delivery, is currently undergoing a dramatic expansion. Diverse effects of different type NPs relating to mammalian reproductive tissues have been demonstrated. Th e objective of this study was to explore the in vitro effects of polymeric nanoparticle poly(ethylene glycol)-blockpolylactide methyl ether (PEG-b-PLA NPs) on functional state and viability of ovarian granulosa cells (GCs), which play an important role in maintaining ovarian function and female fertility...
April 25, 2017: Endocrine Regulations
https://www.readbyqxmd.com/read/28608889/nanoscale-triplet-exciton-diffusion-via-imaging-of-up-conversion-emission-from-single-hybrid-nanoparticles-in-molecular-crystals
#12
Kaishi Narushima, Shuzo Hirata, Martin Vacha
Up-conversion materials composed of donor and acceptor molecules which convert low energy photons into higher energy ones by triplet-triplet annihilation can improve the sensitivity of photocatalysts or the efficiency of solar cells. The use of crystalline materials can lead to a decrease in the up-conversion threshold intensity due to increased diffusion length LT of triplet excitons. Here, we demonstrate direct microscopic imaging of triplet exciton diffusion in polycrystalline films. The generation of high local density of triplet states is achieved by functionalizing alumina nanospheres with donor molecules and dispersing them in the acceptor films...
June 13, 2017: Nanoscale
https://www.readbyqxmd.com/read/28601058/in-vitro-study-of-spions-c595-as-molecular-imaging-probe-for-specific-breast-cancer-mcf-7-cells-detection
#13
Pegah Moradi Khaniabadi, Daryoush Shahbazi-Gahrouei, Amin Malik Shah Abdul Majid, Mohammad Suhaimi Jaafar, Bita Moradi Khaniabadi, Saghar Shahbazi-Gahrouei
Background: Magnetic resonance imaging (MRI) plays an essential role in molecular imaging by delivering the contrast agent into targeted cancer cells. The aim of this study was to evaluate the C595 monoclonal antibody-conjugated superparamagnetic iron oxide nanoparticles (SPIONs-C595) for the detection of breast cancer cell (MCF-7). Methods: The conjugation of monoclonal antibody and nanoparticles was confirmed using X-ray diffraction, transmission electron microscopy, and photon correlation spectroscopy...
June 11, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28597608/advanced-photoacoustic-imaging-applications-of-near-infrared-absorbing-organic-nanoparticles
#14
REVIEW
Yuyan Jiang, Kanyi Pu
Progress of nanotechnology in recent years has stimulated fast development of nanoparticles in biomedical research. Photoacoustic (PA) imaging as an emerging non-invasive technique in molecular imaging has improved imaging depth relative to conventional optical imaging, demonstrating great potential in clinical applications. The convergence of nanotechnology and PA imaging has enabled a broad spectrum of new opportunities in fundamental biology and translation medicine. This review focuses on the recent advances of organic nanoparticles in PA imaging applications...
June 8, 2017: Small
https://www.readbyqxmd.com/read/28593942/dynamic-contrast-enhanced-photoacoustic-imaging-using-photothermal-stimuli-responsive-composite-nanomodulators
#15
Yun-Sheng Chen, Soon Joon Yoon, Wolfgang Frey, Mary Dockery, Stanislav Emelianov
Molecular photoacoustic imaging has shown great potential in medical applications; its sensitivity is normally in pico-to-micro-molar range, dependent on exogenous imaging agents. However, tissue can produce strong background signals, which mask the signals from the imaging agents, resulting in orders of magnitude sensitivity reduction. As such, an elaborate spectral scan is often required to spectrally un-mix the unwanted background signals. Here we show a new single-wavelength photoacoustic dynamic contrast-enhanced imaging technique by employing a stimuli-responsive contrast agent...
June 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28592112/characterization-and-cellular-studies-of-molecular-nanoparticle-of-iron-iii-tannic-complexes-toward-a-low-cost-magnetic-resonance-imaging-agent
#16
Saowalak Krungchanuchat, Titipun Thongtem, Somchai Thongtem, Chalermchai Pilapong
Herein, a new magnetic resonance imaging (MRI) agent based on molecular nanoparticles of iron(III)-tannic complexes (Fe-TA NPs) is reported. The paramagnetic and molecularlike Fe-TA NPs were successfully synthesized at room temperature within a few minutes without the use of any toxic agents or expensive equipment. The coordination states of the Fe-TA NPs were pH-dependent. The r1 relaxivity values of the bis-dominated and tris-dominated structures of the Fe-TA NPs were determined to be 6.31 and 5.24 mM(-1 )s(-1), respectively, by using a Philips Achieva 1...
June 7, 2017: Biointerphases
https://www.readbyqxmd.com/read/28590908/biomimetic-insulin-imprinted-polymer-nanoparticles-as-a-potential-oral-drug-delivery-system
#17
Pijush Kumar Paul, Alongkot Treetong, Roongnapa Suedee
In this study, we investigate molecularly imprinted polymers (MIPs), which form a three-dimensional image of the region at and around the active binding sites of pharmaceutically active insulin or are analogous to b cells bound to insulin. This approach was employed to create a welldefined structure within the nanospace cavities that make up functional monomers by cross-linking. The obtained MIPs exhibited a high adsorption capacity for the target insulin, which showed a significantly higher release of insulin in solution at pH 7...
June 27, 2017: Acta Pharmaceutica
https://www.readbyqxmd.com/read/28589558/potential-applications-and-human-biosafety-of-nanomaterials-used-in-nanomedicine
#18
REVIEW
Hong Su, Yafei Wang, Yuanliang Gu, Linda Bowman, Jinshun Zhao, Min Ding
With the rapid development of nanotechnology, potential applications of nanomaterials in medicine have been widely researched in recent years. Nanomaterials themselves can be used as image agents or therapeutic drugs, and for drug and gene delivery, biological devices, nanoelectronic biosensors or molecular nanotechnology. As the composition, morphology, chemical properties, implant sites as well as potential applications become more and more complex, human biosafety of nanomaterials for clinical use has become a major concern...
June 6, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28569778/single-cell-time-lapse-imaging-of-intracellular-o2-in-response-to-metabolic-inhibition-and-mitochondrial-cytochrome-c-release
#19
Heiko Düssmann, Sergio Perez-Alvarez, Ujval Anilkumar, Dmitri B Papkovsky, Jochen Hm Prehn
The detection of intracellular molecular oxygen (O2) levels is important for understanding cell physiology, cell death, and drug effects, and has recently been improved with the development of oxygen-sensitive probes that are compatible with live cell time-lapse microscopy. We here provide a protocol for the use of the nanoparticle probe MitoImage-MM2 to monitor intracellular oxygen levels by confocal microscopy under baseline conditions, in response to mitochondrial toxins, and following mitochondrial cytochrome-c release...
June 1, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28561568/tunable-semiconducting-polymer-nanoparticles-with-indt-based-conjugated-polymers-for-photoacoustic-molecular-imaging
#20
Thomas Stahl, Robin Bofinger, Ivan Lam, Kealan J Fallon, Peter Johnson, Olumide Ogunlade, Vessela Vassileva, R Barbara Pedley, Paul C Beard, Helen C Hailes, Hugo Bronstein, Alethea B Tabor
Photoacoustic imaging combines both excellent spatial resolution with high contrast and specificity, without the need for patients to be exposed to ionizing radiation. This makes it ideal for the study of physiological changes occurring during tumorigenesis and cardiovascular disease. In order to fully exploit the potential of this technique, new exogenous contrast agents with strong absorbance in the near-infrared range, good stability and biocompatibility, are required. In this paper, we report the formulation and characterization of a novel series of endogenous contrast agents for photoacoustic imaging in vivo...
June 21, 2017: Bioconjugate Chemistry
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