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Biochemical reference values

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https://www.readbyqxmd.com/read/28342316/comparison-of-cytomegalovirus-cmv-specific-neutralization-capacity-of-hyperimmunoglobulin-hig-versus-standard-intravenous-immunoglobulin-ivig-preparations-impact-of-cmv-igg-normalization
#1
Matthias Stefan Schampera, Katrin Schweinzer, Harald Abele, Karl Oliver Kagan, Reinhild Klein, Ingo Rettig, Gerhard Jahn, Klaus Hamprecht
BACKGROUND: Based on a non-randomized study of Nigro et al. (2005) the intravenous administration of hyperimmunoglobulins (HIGs) is applied frequently to women with primary CMV-infection as "off-label use" in Germany. OBJECTIVES: In order to describe their CMV-specific neutralization-capacity in vitro, we analyzed the HIG preparations Cytotect(®), and Cytogam(®) as well as the standard intravenous immunoglobulins (IVIG) Octagam(®), Gamunex(®), Kiovig(®). STUDY DESIGN: We performed short-term cell-free CMV neutralization assays (CFNT) and long-term cell-adapted neutralization-plaque-reduction assays (PRANT)...
March 16, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28331946/-technique-of-proton-and-phosphorous-mr-spectroscopy
#2
M Backens
CLINICAL/METHODICAL ISSUE: Magnetic resonance spectroscopy (MRS) is an important non-invasive method that can reveal the concentration and spatial distribution of particular biochemically relevant tissue metabolites. STANDARD RADIOLOGICAL METHODS: Proton MRS is routinely applicable in the clinical setting providing good quality results even with a moderate magnetic field strength of 1.5 T. Relative values of metabolite concentrations are mostly used for the assessment of metabolic disorders...
March 22, 2017: Der Radiologe
https://www.readbyqxmd.com/read/28331563/%C3%A2-evaluation-of-serum-hbv-viral-load-transaminases-and-histological-features-in-chronic-hbeag-negative-hepatitis-b-patients
#3
Abbas Esmaeelzadeh, Hassan Saadatnia, Bahram Memar, Elham Mokhtari Amirmajdi, Azita Ganji, Ladan Goshayeshi, Zahra Meshkat, Alireza Pasdar, Hassan Vosoughinia, Mohammadreza Farzanehfar, Shahrzad Tehranian, Kamran Ghaffarzadehgan, Farnood Rajabzadeh, Mitra Ahadi
AIM: To evaluate the association between biochemical, virologic and histologic features in patients with HBeAg-negative chronic hepatitis B (CHB). BACKGROUND: Hepatitis-B e-antigen (HBeAg)-negative is common in Iran, is progressive with poor prognosis. Therefore, it seems necessary to perform a comprehensive evaluation of different spectrum of laboratory measurements accompanying histological findings. METHODS: HBeAg- negative CHB patients referring to two university hospitals during two years were enrolled...
2017: Gastroenterology and Hepatology From Bed to Bench
https://www.readbyqxmd.com/read/28325824/persistence-of-myopathy-in-cushing-s-syndrome-evaluation-of-the-german-cushing-s-registry
#4
Christina Maria Berr, Mareike R Stieg, Timo Deutschbein, Marcus Quinkler, Ralf Schmidmaier, Andrea Osswald, Nicole Reisch, Katrin Ritzel, Christina Dimopoulou, Julia Fazel, Stefanie Hahner, Gunter K Stalla, Felix Beuschlein, Martin Reincke
BACKGROUND: Cushing's syndrome (CS) is characterized by an excessive secretion of glucocorticoids that results in a characteristic clinical phenotype. One feature of clinical hypercortisolism is breakdown of protein metabolism translating into clinical consequences including glucocorticoid induced myopathy. While surgery is effective in control of cortisol excess, the effect of biochemical remission on muscular function is yet unclear. METHODS: In a cross-sectional study we analyzed 47 patients with CS during the florid phase (ActiveCS)...
March 21, 2017: European Journal of Endocrinology
https://www.readbyqxmd.com/read/28302053/a-fast-and-efficient-python-library-for-interfacing-with-the-biological-magnetic-resonance-data-bank
#5
Andrey Smelter, Morgan Astra, Hunter N B Moseley
BACKGROUND: The Biological Magnetic Resonance Data Bank (BMRB) is a public repository of Nuclear Magnetic Resonance (NMR) spectroscopic data of biological macromolecules. It is an important resource for many researchers using NMR to study structural, biophysical, and biochemical properties of biological macromolecules. It is primarily maintained and accessed in a flat file ASCII format known as NMR-STAR. While the format is human readable, the size of most BMRB entries makes computer readability and explicit representation a practical requirement for almost any rigorous systematic analysis...
March 17, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28299435/genome-wide-identification-classification-and-functional-analysis-of-the-basic-helix-loop-helix-transcription-factors-in-the-cattle-bos-taurus
#6
Fengmei Li, Wuyi Liu
The basic helix-loop-helix (bHLH) transcription factors (TFs) form a huge superfamily and play crucial roles in many essential developmental, genetic, and physiological-biochemical processes of eukaryotes. In total, 109 putative bHLH TFs were identified and categorized successfully in the genomic databases of cattle, Bos Taurus, after removing redundant sequences and merging genetic isoforms. Through phylogenetic analyses, 105 proteins among these bHLH TFs were classified into 44 families with 46, 25, 14, 3, 13, and 4 members in the high-order groups A, B, C, D, E, and F, respectively...
March 15, 2017: Mammalian Genome: Official Journal of the International Mammalian Genome Society
https://www.readbyqxmd.com/read/28289429/hematological-and-plasma-biochemical-parameters-in-a-wild-population-of-naja-naja-linnaeus-1758-in-sri-lanka
#7
Duminda S B Dissanayake, Lasanthika D Thewarage, Rathnayake M P Manel Rathnayake, Senanayake A M Kularatne, Jamburagoda G Shirani Ranasinghe, Rajapakse P V Jayantha Rajapakse
BACKGROUND: Hematological studies of any animal species comprise an important diagnostic method in veterinary medicine and an essential tool for the conservation of species. In Sri Lanka, this essential technique has been ignored in studies of many species including reptiles. The aim of the present work was to establish a reference range of hematological values and morphological characterization of wild spectacled cobras (Naja naja) in Sri Lanka in order to provide a diagnostic tool in the assessment of health condition in reptiles and to diagnose diseases in wild populations...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28281516/-features-of-a-necrotic-and-inflammatory-process-in-different-forms-of-nonalcoholic-fatty-liver-disease
#8
I V Kurbatova, O P Dudanova
AIM: To identify the features of development of a necrotic and inflammatory process in different forms of nonalcoholic fatty liver disease (NAFLD), by comparatively analyzing a full set of clinical and laboratory parameters, including the cytokine status and the expression level of enzyme genes controlling the apoptosis of peripheral leukocytes. SUBJECTS AND METHODS: 86 patients with NAFLD, including 8 (9.3%) with hepatic steatosis (HS), 70 (81.4%) with nonalcoholic steatohepatitis (NASH), 40, 19, and 11 with mild, moderate, and high disease activity, respectively, and 8 (9...
2017: Terapevticheskiĭ Arkhiv
https://www.readbyqxmd.com/read/28250659/antidiabetic-effects-of-aqueous-and-dichloromethane-methanol-stem-bark-extracts-of-pterocarpus-soyauxii-taub-papilionaceae-on-streptozotocin-induced-diabetic-rats
#9
Marie Claire Tchamadeu, Paul Désiré Djomeni Dzeufiet, Nelly Blaes, Jean-Pierre Girolami, Pierre Kamtchouing, Théophile Dimo
AIM OF THE STUDY: The aim is to evaluate the hypoglycemic and antidiabetic effects of aqueous and CH2Cl2/CH3OH stem bark extracts of Pterocarpus soyauxii Taub in normal and diabetic rats. MATERIALS AND METHODS: Streptozotocin (STZ)-induced diabetic and normal adult Wistar rats were orally administered with aqueous and CH2Cl2/CH3OH plant extracts of P. soyauxii at various doses (38-300 mg/kg) in a single administration. In addition, STZ-induced diabetic rats received prolonged daily administration for 14 days...
January 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/28249232/prevotella-intermedia-infection-causing-acute-and-complicated-aortitis-a-case-report
#10
C Boersma, L M Kampschreur, H Buter, B M Doorenbos, P Klinkert, G G Koning
INTRODUCTION: Aortitis is a general term that refers to all conditions involving an inflammation of the aortic wall. This case report describes the surgical approach of a patient with infectious and symptomatic aortitis caused by the rare vector Prevotella intermedia. PRESENTATION OF CASE: A 44-year old male patient was admitted with fever and general discomfort after a period of sore throat in a non-teaching hospital. After two weeks he developed acute abdominal and back pain accompanied by sweating and elevated infection parameters...
February 20, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28247485/is-the-first-urinary-albumin-creatinine-ratio-acr-in-women-with-suspected-pre-eclampsia-a-prognostic-factor-for-maternal-and-neonatal-adverse-outcome-a-retrospective-cohort-study
#11
Eleni G Elia, Amy O Robb, Karla Hemming, Malcolm J Price, Richard D Riley, Anna French-Constant, Fiona C Denison, Mark D Kilby, Rachel K Morris, Sarah J Stock
INTRODUCTION: The aim of this study was to determine the prognostic value of the first urinary albumin/creatinine ratio (ACR) for adverse maternal and neonatal outcomes and how it relates to other prognostic factors. MATERIAL AND METHODS: We performed a retrospective cohort study from December 2009 to February 2012 with analysis of demographic, clinical and biochemical data from two obstetric day assessment units in hospitals in Southeast Scotland. We included 717 pregnant women, with singleton pregnancies after 20 weeks gestation, referred for evaluation of suspected pre-eclampsia and having their first ACR performed...
March 1, 2017: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/28220408/guanidinoacetate-methyltransferase-activity-in-lymphocytes-for-a-fast-diagnosis
#12
Lisette M Berends, Eduard A Struys, Birthe Roos, Ulbe Holwerda, Erwin E W Jansen, Gajja S Salomons, Mirjam M C Wamelink
INTRODUCTION: Guanidinoacetate methyltransferase (GAMT) deficiency is an inborn error of metabolism (IEM), clinically characterized by intellectual disability, developmental delay, seizures, and movement disorders. Biochemical diagnosis of GAMT deficiency is based on the measurement of creatine and guanidinoacetate in urine, plasma, or CSF and is confirmed genetically by DNA analysis or by enzyme assay in lymphoblasts or fibroblasts. To obtain enough cells, these cells need to be cultured for at least 1 month...
February 21, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28217151/infantile-tremor-syndrome-role-of-vitamin-b12-revisited
#13
Rajesh Gupta, Jagdish Mandliya, Pavan Sonker, Vandana Patil, Manish Agrawal, Ashish Pathak
OBJECTIVE: To study the role of Vitamin B12 as an etiological factor in patients of infantile tremor syndrome (ITS). METHODS: Twelve consecutive admissions of children diagnosed clinically as ITS were assessed. Assessment was done using a predefined pro forma to document patient demographic factors, general examination, systemic examination as well as relevant hematological and biochemical investigations. RESULTS: Out of the 12 cases of ITS, 6 were males and 6 were females...
October 2016: Journal of Pediatric Neurosciences
https://www.readbyqxmd.com/read/28168177/effect-of-nutrition-counseling-program-on-weight-control-in-obese-university-students
#14
Bo Ryeong Kim, Seung Yeon Seo, Na Gyeong Oh, Jung-Sook Seo
This study was conducted to investigate the effect of nutrition counseling program and related factors on weight control for obese university students. Subjects were 24 students with a body mass index (BMI) of 25 or above. The program was conducted from September 16th to November 18th, 2015. Change of body composition, blood index and nutrient intake were observed in subjects before and after the program. The average age of the subjects was 23.2 years old and the percentage of male and female was 66.7% and 33...
January 2017: Clinical Nutrition Research
https://www.readbyqxmd.com/read/28164550/theoretical-and-experimental-evidence-in-mixed-serum-samples-as-natural-reference-materials-for-measuring-activity-of-biomacromolecules-in-serum
#15
Yang Guang, Dong Feng, Li Yuzhong, Liu Hui
BACKGROUND: The target substances of biomacromolecules are primarily unstable in biological, chemical, and immunological activity, whereby, the standardized substances are obtained with considerable difficulty. We explored the possibility of using a mixed serum sample as a natural reference material for measurement of biomacromolecules in serum. METHODS: Eight serum samples were selected randomly from a clinical laboratory. Then, 2, 4, and 8 serum samples were mixed (in equal quantity) resulting in 4, 2, and 1 sample groups, respectively, to compare mean measurement results of biochemical indices between single samples and mixed samples...
December 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28154581/detection-of-methicillin-resistant-staphylococcus-aureus-and-determination-of-minimum-inhibitory-concentration-of-vancomycin-for-staphylococcus-aureus-isolated-from-pus-wound-swab-samples-of-the-patients-attending-a-tertiary-care-hospital-in-kathmandu-nepal
#16
Raghabendra Adhikari, Narayan Dutt Pant, Sanjeev Neupane, Mukesh Neupane, Roshan Bhattarai, Sabita Bhatta, Raina Chaudhary, Binod Lekhak
The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S. aureus isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28132763/alternative-indices-of-glucose-homeostasis-as-biochemical-diagnostic-tests-for-abnormal-glucose-tolerance-in-an-african-setting
#17
Andre Pascal Kengne, Rajiv T Erasmus, Naomi S Levitt, Tandi E Matsha
AIMS: Accurate diabetes diagnosis is important in Africa, where rates are increasing, and the disease largely undiagnosed. The cumbersome oral glucose tolerance test (OGTT) remains the reference standard, while alternative diagnostic methods are not yet established in Africans. We assessed the ability of fasting plasma glucose (FPG), HbA1c and fructosamine, to diagnose OGTT-based abnormal glucose tolerance in mixed-ancestry South Africans. METHODS: Mixed-ancestry adults, residing in Cape Town were examined between February and November 2015...
April 2017: Primary Care Diabetes
https://www.readbyqxmd.com/read/28121522/-18-f-fluorocholine-pet-mr-imaging-in-patients-with-primary-hyperparathyroidism-and-inconclusive-conventional-imaging-a-prospective-pilot-study
#18
Wouter P Kluijfhout, Jesse D Pasternak, Jessica E Gosnell, Wen T Shen, Quan-Yang Duh, Menno R Vriens, Bart de Keizer, Thomas A Hope, Christine M Glastonbury, Miguel H Pampaloni, Insoo Suh
Purpose To investigate the performance of flourine 18 ((18)F) fluorocholine (FCH) positron emission tomography (PET)/magnetic resonance (MR) imaging in patients with hyperparathyroidism and nonlocalized disease who have negative or inconclusive results at ultrasonography (US) and technetium 99m ((99m)Tc) sestamibi scintigraphy. Materials and Methods This study was approved by the institutional review board. Between May and December 2015, 10 patients (mean age, 70.4 years; range, 58-82 years) with biochemical primary hyperparathyroidism and inconclusive results at US and (99m)Tc sestamibi scintigraphy were prospectively enrolled...
January 25, 2017: Radiology
https://www.readbyqxmd.com/read/28115177/prediction-of-lymph-node-parasite-load-from-clinical-data-in-dogs-with-leishmaniasis-an-application-of-radial-basis-artificial-neural-networks
#19
Rafaela Beatriz Pintor Torrecilha, Yuri Tani Utsunomiya, Luís Fábio da Silva Batista, Anelise Maria Bosco, Cáris Maroni Nunes, Paulo César Ciarlini, Márcia Dalastra Laurenti
Quantification of Leishmania infantum load via real-time quantitative polymerase chain reaction (qPCR) in lymph node aspirates is an accurate tool for diagnostics, surveillance and therapeutics follow-up in dogs with leishmaniasis. However, qPCR requires infrastructure and technical training that is not always available commercially or in public services. Here, we used a machine learning technique, namely Radial Basis Artificial Neural Network, to assess whether parasite load could be learned from clinical data (serological test, biochemical markers and physical signs)...
January 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28111337/worse-outcomes-of-patients-with-hfe-hemochromatosis-with-persistent-increases-in-transferrin-saturation-during-maintenance-therapy
#20
Edouard Bardou-Jacquet, Fabrice Lainé, Pascal Guggenbuhl, Jeff Morcet, Caroline Jézéquel, Dominique Guyader, Romain Moirand, Yves Deugnier
BACKGROUND & AIMS: Even if patients with hemochromatosis maintain low serum levels of ferritin, they still have an increased risk of general and joint symptoms, which reduce quality of life. This could be related to persistently increased transferrin saturation. We assessed whether duration of exposure to increased transferrin saturation during maintenance therapy is associated with more severe general and joint symptoms. METHODS: We performed a longitudinal cohort study of 266 individuals homozygous for the C282Y substitution in HFE, seen at a tertiary reference center in Rennes, France, and followed for 3 or more years after initial iron removal...
January 19, 2017: Clinical Gastroenterology and Hepatology
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