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Biochemical reference values

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https://www.readbyqxmd.com/read/29159585/kfoa-the-udp-glucose-4-epimerase-of-escherichia-coli-strain-o5-k4-h4-shows-preference-for-acetylated-substrates
#1
Hao-Miao Zhu, Bin Sun, Yi-Jun Li, Dan-Hua Meng, Shuang Zheng, Ting-Ting Wang, Feng-Shan Wang, Ju-Zheng Sheng
Capsule of Escherichia coli O5:K4:H4 is formed of a chondroitin-repeat disaccharide unit of glucuronic acid (GlcA)-N-acetylgalactosamine (GalNAc). This polysaccharide, commonly referred to as K4CP, is a potentially important source of precursors for chemoenzymatic or bioengineering synthesis of chondroitin sulfate. KfoA, encoded by a gene from region 2 of the K4 capsular gene cluster, shows high homology to the UDP-glucose-4-epimerase (GalE) from E. coli. KfoA is reputed to be responsible for uridine 5'-diphosphate-N-acetylgalactosamine (UDP-GalNAc) supply for K4CP biosynthesis in vivo, but it has not been biochemically characterized...
November 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29153517/rapid-species-and-subspecies-specific-level-classification-and-identification-of-lactobacillus-casei-group-members-using-maldi-biotyper-combined-with-clinprotools
#2
Chien-Hsun Huang, Lina Huang
Using common taxonomic methods such as 16S rDNA sequencing and physiological and biochemical analysis to identify members of the Lactobacillus casei group (LCG) is time-consuming, expensive and inaccurate. In this study, we applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapid discriminate LCG strains by creating an analytical in-house database (IHDB) and to develop a classification model for subspecies-level differentiation based on MS biomarkers using ClinProTools bioinformatics software (Bruker Daltonics, Billerica, MA)...
November 15, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29134929/mucilaginibacter-panaciglaebae-sp-nov-isolated-from-soil-of-a-ginseng-field
#3
Soon Youl Lee, Muhammad Zubair Siddiqi, Sang Yong Kim, Hong Shan Yu, Jae Hak Lee, Wan-Taek Im
A Gram-reaction-negative, strictly aerobic, non-motile and rod-shaped bacterium, designated strain BXN5-31(T), was isolated from soil of a ginseng field, and its taxonomic position was investigated using a polyphasic approach. Strain BXN5-31(T) grew at 18-37 °C and at pH 6.0-8.0 on R2A medium. Based on 16S rRNA gene sequence similarity, strain BXN5-31(T) was shown to belong to the genus Mucilaginibacter and was closely related to Mucilaginibactersoyangensis HME6664(T), Mucilaginibacterximonensis XM-003(T) and Mucilaginibacterpuniceus WS71(T)...
November 14, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29126424/salivary-ph-calcium-phosphorus-and-selected-enzymes-in-healthy-dogs-a-pilot-study
#4
Ilaria Iacopetti, Anna Perazzi, Tamara Badon, Silvia Bedin, Barbara Contiero, Rebecca Ricci
BACKGROUND: Saliva in dogs, as in humans, is a complex fluid secreted by different salivary glands in the oral cavity to protect the oral mucosa and teeth. The use of saliva as a substitute for blood in diagnosing and prognosticating disease in humans is widely accepted. Salivary biochemistry has also been used as a marker for periodontal disease in humans. No studies have as yet investigated the relation between salivary biochemistry and periodontal disease in dogs, however; neither has the salivary composition of healthy dogs with no oral disease been assessed...
November 10, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29119481/prevalence-of-rheumatoid-cachexia-assessed-by-bioelectrical-impedance-vector-analysis-and-its-relation-with-physical-function
#5
Cira Santillán-Díaz, Noemi Ramírez-Sánchez, Rolando Espinosa-Morales, Arturo Orea-Tejeda, Luis Llorente, Gerardo Rodríguez-Guevara, Lilia Castillo-Martínez
Rheumatoid arthritis (RA) patients frequently have changes in their body composition, with a decrease in muscle mass and an increase in fat mass, a syndrome that is termed rheumatoid cachexia (RC). The prevalence of this nutritional alteration is not well known; there is as yet no consensus, seeing as it depends on the methods, techniques, and cutoff points that are used for its diagnosis. The main aim of this study was to identify RC through assessment by bioelectrical impedance vector analysis (BIVA) and its association with metabolic causes, physical function, and the main disease status, among others...
November 8, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/29116560/preoperative-and-postoperative-assessments-of-biochemical-parameters-in-patients-with-severe-obesity-undergoing-laparoscopic-sleeve-gastrectomy
#6
Deborah M Gregory, Laurie K Twells, Kendra K Lester, William K Midodzi, Mette Rode Pedersen, David Pace, Chris Smith, Darrell Boone, Edward W Randell, Christopher S Kovacs
BACKGROUND: The study aim was to determine the prevalence of abnormal serum biochemistries associated with micronutrient deficiencies before and after laparoscopic sleeve gastrectomy (LSG). METHODS: Two hundred and one patients had LSG surgery between May 2011 and May 2014. Using a prospective cohort study design, data were collected on ferritin, hemoglobin (Hgb), mean cell volume (MCV), calcium, albumin, 25-hydroxyvitamin D (25-OH-D), PTH, and vitamin B12 with follow-up of 75...
November 8, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29107010/combining-high-sensitivity-cardiac-troponin-and-b-type-natriuretic-peptide-in-the-detection-of-inducible-myocardial-ischemia
#7
Christian Puelacher, Max Wagener, Ursina Honegger, Mustafa Assadian, Nicolas Schaerli, Deborah Mueller, Ivo Strebel, Raphael Twerenbold, Jasper Boeddinghaus, Thomas Nestelberger, Karin Wildi, Zaid Sabti, Lorraine Sazgary, Patrick Badertscher, Jeanne du Fay de Lavallaz, Stella Marbot, Christoph Kaiser, Damian Wild, Michael J Zellweger, Tobias Reichlin, Christian Mueller
BACKGROUND: Single biomarker approaches provide only moderate accuracy in the non-invasive detection of exercise-induced myocardial ischemia. We therefore assessed the combination of the two most promising single biomarkers: high-sensitivity cardiac troponin I (hs-cTnI) and B-type natriuretic peptide (BNP). METHODS: Consecutive patients with suspected myocardial ischemia referred to stress myocardial perfusion single-photon emission tomography imaging (MPI) were enrolled...
November 8, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/29105797/-99m-tc-mip-1404-spect-ct-for-the-detection-of-psma-positive-lesions-in-225-patients-with-biochemical-recurrence-of-prostate-cancer
#8
Christian Schmidkonz, Claudia Hollweg, Michael Beck, Julia Reinfelder, Theresa I Goetz, James C Sanders, Daniela Schmidt, Olaf Prante, Tobias Bäuerle, Alexander Cavallaro, Michael Uder, Bernd Wullich, Peter Goebell, Torsten Kuwert, Philipp Ritt
BACKGROUND: (99m) Tc-MIP-1404 (Progenics Pharmaceuticals, Inc., New York, NY) is a novel, SPECT-compatible (99m) Tc-labeled PSMA inhibitor for the detection of prostate cancer. We present results of its clinical use in a cohort of 225 men with histologically confirmed prostate cancer referred for workup of biochemical relapse. METHODS: From April 2013 to April 2017, (99m) Tc-MIP1404-scintigraphy was performed in 225 patients for workup of PSA biochemical relapse of prostate cancer...
November 6, 2017: Prostate
https://www.readbyqxmd.com/read/29096421/glucocorticosteroids-for-people-with-alcoholic-hepatitis
#9
REVIEW
Chavdar S Pavlov, Daria L Varganova, Giovanni Casazza, Emmanuel Tsochatzis, Dimitrinka Nikolova, Christian Gluud
BACKGROUND: Alcoholic hepatitis is a form of alcoholic liver disease, characterised by steatosis, necroinflammation, fibrosis, and potential complications to the liver disease. Typically, alcoholic hepatitis presents in people between 40 and 50 years of age. Alcoholic hepatitis can be resolved if people abstain from drinking, but the risk of death will depend on the severity of the liver damage and abstinence from alcohol. Glucocorticosteroids are used as anti-inflammatory drugs for people with alcoholic hepatitis...
November 2, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29082604/-68-ga-psma-pet-ct-for-the-detection-of-bone-metastases-in-prostate-cancer-a-systematic-review-of-the-published-literature
#10
Helle D Zacho, Julie B Nielsen, Uwe Haberkorn, Louise Stenholt, Lars J Petersen
BACKGROUND: (68) Ga-labelled prostate-specific membrane antigen (PSMA) is a promising PET ligand for the detection of prostate cancer. Little attention has been given to the ability of (68) Ga-PSMA PET/CT to detect malignant bone lesions and whether this approach is superior to existing bone imaging modalities. AIM: To review the existing data of (68) Ga-PSMA PET/CT for the diagnosis of bone metastases in prostate cancer. METHODS: Systematic review of the peer-reviewed literature...
October 29, 2017: Clinical Physiology and Functional Imaging
https://www.readbyqxmd.com/read/29070545/laboratory-methodologies-for-indicators-of-iron-status-strengths-limitations-and-analytical-challenges
#11
REVIEW
Christine M Pfeiffer, Anne C Looker
Biochemical assessment of iron status relies on serum-based indicators, such as serum ferritin (SF), transferrin saturation, and soluble transferrin receptor (sTfR), as well as erythrocyte protoporphyrin. These indicators present challenges for clinical practice and national nutrition surveys, and often iron status interpretation is based on the combination of several indicators. The diagnosis of iron deficiency (ID) through SF concentration, the most commonly used indicator, is complicated by concomitant inflammation...
October 25, 2017: American Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29062181/micronutrients-drift-during-daily-and-weekly-iron-supplementation-in-non-anaemic-and-anaemic-pregnancy
#12
Hari Shankar, Neeta Kumar, Rajat Sandhir, Suneeta Mittal, Tulsi Adhikari, Arun Kumar, D N Rao
AIMS: Pregnancy is a phenomenon associated with dynamic changes in physical, mental and biochemical status of body and demands increased nutritional intake for developing foetus. The level of various micronutrients which act as co-factors for antioxidant enzymes or it-self as antioxidants gets altered with the progression of pregnancy. The present longitudinal study summarized the trend of selected micronutrients level in anaemic (AP) and non-anaemic primigravida (NAP) supplemented with daily and weekly oral iron folic acid (IFA) tablet during pregnancy and postpartum...
October 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/29055982/vitamin-a-status-and-its-determinants-in-patients-with-cystic-fibrosis
#13
Ewa Sapiejka, Patrycja Krzyżanowska, Dariusz Walkowiak, Ewa Wenska-Chyży, Mariusz Szczepanik, Szczepan Cofta, Andrzej Pogorzelski, Wojciech Skorupa, Jarosław Walkowiak
BACKGROUND: Routine administration of vitamin A, recommended in CF patients, can help to prevent its deficiency. However, high vitamin A supplementation may lead to its excessive level and possible toxicity. Therefore, the aim of the present study was to assess the status of vitamin A and the determinants of its body resources in CF patients. METHODS: In 196 CF patients aged from 4 months to 47 years, the following parameters  were analysed: nutritional status (standardized body weight and height, serum albumin concentration) and clinical expression of disease (lung function - spirometry; biochemical markers of liver function - ALT, AST, GGT; respiratory tract colonization by Pseudomonas aeruginosa; diabetes; cirrhosis, non-cirrhotic liver disease; exocrine pancreatic function - fecal elastase-1 concentration; blood clotting -  INR  and  vitamin  A supplementation)...
July 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29038888/simultaneous-whole-body-18-f-psma-1007-pet-mri-with-integrated-high-resolution-multiparametric-imaging-of-the-prostatic-fossa-for-comprehensive-oncological-staging-of-patients-with-prostate-cancer-a-pilot-study
#14
Martin T Freitag, Claudia Kesch, Jens Cardinale, Paul Flechsig, Ralf Floca, Matthias Eiber, David Bonekamp, Jan P Radtke, Clemens Kratochwil, Klaus Kopka, Markus Hohenfellner, Albrecht Stenzinger, Heinz-Peter Schlemmer, Uwe Haberkorn, Frederik Giesel
INTRODUCTION: The aim of the present study was to explore the clinical feasibility and reproducibility of a comprehensive whole-body (18)F-PSMA-1007-PET/MRI protocol for imaging prostate cancer (PC) patients. METHODS: Eight patients with high-risk biopsy-proven PC underwent a whole-body PET/MRI (3 h p.i.) including a multi-parametric prostate MRI after (18)F-PSMA-1007-PET/CT (1 h p.i.) which served as reference. Seven patients presented with non-treated PC, whereas one patient presented with biochemical recurrence...
October 16, 2017: European Journal of Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/29026467/chromogranin-a-as-a-biochemical-marker-for-neuroendocrine-tumors-a-single-center-experience-at-royal-hospital-oman
#15
Elham S Al-Risi, Fatma S Al-Essry, Waad-Allah S Mula-Abed
OBJECTIVES: To evaluate the significance of serum chromogranin A (CgA) status in patients with and without different neuroendocrine tumors (NETs) by conducting a retrospective assessment of the diagnostic utility and limitations of CgA as a biomarker for NETs in a tertiary care hospital in Oman. METHODS: We conducted a retrospective analysis of CgA requests referred to the Clinical Biochemistry Laboratory, Royal Hospital, Oman over a 24-month period (April 2012 to March 2014)...
September 2017: Oman Medical Journal
https://www.readbyqxmd.com/read/29017389/the-canadian-laboratory-initiative-on-pediatric-reference-intervals-a-caliper-white-paper
#16
Khosrow Adeli, Victoria Higgins, Karin Trajcevski, Nicole White-Al Habeeb
Laboratory investigations provide physicians with objective data to aid in disease diagnosis, clinical decision making, and patient follow up. Clinical interpretation of laboratory test results relies heavily on the availability of appropriate population-based reference intervals (i.e. normative values) or decision limits developed through clinical outcome studies. Although reference intervals are fundamental to accurate laboratory test interpretation, and thus critically important to healthcare, the need for sound evidence-based reference intervals has been largely overlooked, particularly in the pediatric population...
October 11, 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/28992911/whole-body-vibration-exercise-on-hematology-and-serum-biochemistry-in-healthy-dogs
#17
Ivan Felismino Charas Santos, Sheila Canevese Rahal, Jussara Shimono, Miriam Tsunemi, Regina Takahira, Carlos Roberto Teixeira
The present study aimed to evaluate the influence of whole-body vibration (WBV) exercise on hematology and serum biochemistry in healthy dogs. Ten intact beagle dogs, 3 females, and 7 males, with a mean age of 3 years, and mean body mass of 14.3kg, body condition score between 4.0/9 and 4.5/9 were evaluated. The WBV sessions were done with the dog standing up on all 4 feet on a vibrating platform. Daily session of 30Hz for 5 minutes, followed by 50Hz for 5 minutes and finishing with 30Hz for 5 minutes was accomplished for 5 days...
June 2017: Topics in Companion Animal Medicine
https://www.readbyqxmd.com/read/28975108/comparing-the-values-of-intact-parathormone-and-1-84-pth-to-predict-hyperparathyroidism-in-hemodialysis-patients
#18
Aria Jenabi, Mosadegh Jabbari, Hossein Ziaie
BACKGROUND: Secondary hyperparathyroidism (SHPT) is a common complication of chronic kidney disease (CKD) leading high mortality and even long-term morbidity. SHPT is manifested by elevation of parathyroid hormone (PTH) and accurate determining the level of serum PTH is very essential for early diagnosis of SHPT secondary to CKD. It is very important to match the values obtained for intact parathormone (iPTH) and 1- 84 PTH with the minimized measurement bias. OBJECTIVES: The present study aimed to first determine the agreement value between the iPTH and 1- 84 PTH measures in patients with hyperparathyroidism secondary to endstage renal disease under chronic hemodialysis...
July 2017: Journal of Nephropathology
https://www.readbyqxmd.com/read/28965606/mineral-status-and-superoxide-dismutase-enzyme-activity-in-alzheimer-s-disease
#19
Gilmara Péres Rodrigues, Silvia Maria Franciscato Cozzolino, Dilina do Nascimento Marreiro, Daniele Rodrigues Carvalho Caldas, Kelcylene Gomes da Silva, Kaluce Gonçalves de Sousa Almondes, José Machado Moita Neto, José Alexandre Coelho Pimentel, Cecília Maria Resende Gonçalves de Carvalho, Nadir do Nascimento Nogueira
The study evaluated the dietary intake of zinc and copper, as measured by plasma and erythrocyte concentrations, the Cu/Zn ratio and measure the erythrocyte superoxide dismutase enzyme (eSOD) activity and the relationship between these markers and the degree of dementia in elderly individuals with and without Alzheimer's Disease (AD). A total of 93 elderly people aged 60-94 years were divided into two groups: with AD (n=44) and without AD (n=49). The NINCDS-ADRDA criteria were used for diagnosing AD, and dementia staging was determined using the Clinical Dementia Rating (CDR) scale...
December 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/28929047/determining-frequency-of-genes-of-ctx-m-and-ctx-m-15-of-producing-enterobacteriaceae-of-isolated-extended-spectrum-beta-lactamases-from-clinical-samples-of-patients-referred-to-training-hospitals-of-medical-sciences-university-khorramabad-iran
#20
Seyed Mojtaba Mosavian, Azam Rezvani-Rad
OBJECTIVE: The purpose of conducting this research was evaluation of the frequency of extended-spectrum beta-lactamases (ESBLs) in separated Enterobacteriaceae isolates from clinical samples in Khorramabad city and determination of their antimicrobial resistance pattern. MATERIALS AND METHODS: In this study, 240 isolates belonging to Enterobacteriaceae family were collected in time duration between March and June in 2014. The isolates were identified by standard biochemical tests...
April 2017: International Journal of Pharmaceutical Investigation
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