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Biochemical reference values

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https://www.readbyqxmd.com/read/28220408/guanidinoacetate-methyltransferase-activity-in-lymphocytes-for-a-fast-diagnosis
#1
Lisette M Berends, Eduard A Struys, Birthe Roos, Ulbe Holwerda, Erwin E W Jansen, Gajja S Salomons, Mirjam M C Wamelink
INTRODUCTION: Guanidinoacetate methyltransferase (GAMT) deficiency is an inborn error of metabolism (IEM), clinically characterized by intellectual disability, developmental delay, seizures, and movement disorders. Biochemical diagnosis of GAMT deficiency is based on the measurement of creatine and guanidinoacetate in urine, plasma, or CSF and is confirmed genetically by DNA analysis or by enzyme assay in lymphoblasts or fibroblasts. To obtain enough cells, these cells need to be cultured for at least 1 month...
February 21, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28217151/infantile-tremor-syndrome-role-of-vitamin-b12-revisited
#2
Rajesh Gupta, Jagdish Mandliya, Pavan Sonker, Vandana Patil, Manish Agrawal, Ashish Pathak
OBJECTIVE: To study the role of Vitamin B12 as an etiological factor in patients of infantile tremor syndrome (ITS). METHODS: Twelve consecutive admissions of children diagnosed clinically as ITS were assessed. Assessment was done using a predefined pro forma to document patient demographic factors, general examination, systemic examination as well as relevant hematological and biochemical investigations. RESULTS: Out of the 12 cases of ITS, 6 were males and 6 were females...
October 2016: Journal of Pediatric Neurosciences
https://www.readbyqxmd.com/read/28168177/effect-of-nutrition-counseling-program-on-weight-control-in-obese-university-students
#3
Bo Ryeong Kim, Seung Yeon Seo, Na Gyeong Oh, Jung-Sook Seo
This study was conducted to investigate the effect of nutrition counseling program and related factors on weight control for obese university students. Subjects were 24 students with a body mass index (BMI) of 25 or above. The program was conducted from September 16th to November 18th, 2015. Change of body composition, blood index and nutrient intake were observed in subjects before and after the program. The average age of the subjects was 23.2 years old and the percentage of male and female was 66.7% and 33...
January 2017: Clinical Nutrition Research
https://www.readbyqxmd.com/read/28164550/theoretical-and-experimental-evidence-in-mixed-serum-samples-as-natural-reference-materials-for-measuring-activity-of-biomacromolecules-in-serum
#4
Yang Guang, Dong Feng, Li Yuzhong, Liu Hui
BACKGROUND: The target substances of biomacromolecules are primarily unstable in biological, chemical, and immunological activity, whereby, the standardized substances are obtained with considerable difficulty. We explored the possibility of using a mixed serum sample as a natural reference material for measurement of biomacromolecules in serum. METHODS: Eight serum samples were selected randomly from a clinical laboratory. Then, 2, 4, and 8 serum samples were mixed (in equal quantity) resulting in 4, 2, and 1 sample groups, respectively, to compare mean measurement results of biochemical indices between single samples and mixed samples...
December 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28154581/detection-of-methicillin-resistant-staphylococcus-aureus-and-determination-of-minimum-inhibitory-concentration-of-vancomycin-for-staphylococcus-aureus-isolated-from-pus-wound-swab-samples-of-the-patients-attending-a-tertiary-care-hospital-in-kathmandu-nepal
#5
Raghabendra Adhikari, Narayan Dutt Pant, Sanjeev Neupane, Mukesh Neupane, Roshan Bhattarai, Sabita Bhatta, Raina Chaudhary, Binod Lekhak
The present study was conducted to evaluate the performance of cefoxitin disc diffusion method and oxacillin broth microdilution method for detection of methicillin resistant S. aureus (MRSA), taking presence of mecA gene as reference. In addition, inducible clindamycin resistance and beta-lactamase production were studied and minimum inhibitory concentration (MIC) of vancomycin for S. aureus isolates was determined. A total of 711 nonrepeated pus/wound swab samples from different anatomic locations were included in the study...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28132763/alternative-indices-of-glucose-homeostasis-as-biochemical-diagnostic-tests-for-abnormal-glucose-tolerance-in-an-african-setting
#6
Andre Pascal Kengne, Rajiv T Erasmus, Naomi S Levitt, Tandi E Matsha
AIMS: Accurate diabetes diagnosis is important in Africa, where rates are increasing, and the disease largely undiagnosed. The cumbersome oral glucose tolerance test (OGTT) remains the reference standard, while alternative diagnostic methods are not yet established in Africans. We assessed the ability of fasting plasma glucose (FPG), HbA1c and fructosamine, to diagnose OGTT-based abnormal glucose tolerance in mixed-ancestry South Africans. METHODS: Mixed-ancestry adults, residing in Cape Town were examined between February and November 2015...
January 26, 2017: Primary Care Diabetes
https://www.readbyqxmd.com/read/28121522/-18-f-fluorocholine-pet-mr-imaging-in-patients-with-primary-hyperparathyroidism-and-inconclusive-conventional-imaging-a-prospective-pilot-study
#7
Wouter P Kluijfhout, Jesse D Pasternak, Jessica E Gosnell, Wen T Shen, Quan-Yang Duh, Menno R Vriens, Bart de Keizer, Thomas A Hope, Christine M Glastonbury, Miguel H Pampaloni, Insoo Suh
Purpose To investigate the performance of flourine 18 ((18)F) fluorocholine (FCH) positron emission tomography (PET)/magnetic resonance (MR) imaging in patients with hyperparathyroidism and nonlocalized disease who have negative or inconclusive results at ultrasonography (US) and technetium 99m ((99m)Tc) sestamibi scintigraphy. Materials and Methods This study was approved by the institutional review board. Between May and December 2015, 10 patients (mean age, 70.4 years; range, 58-82 years) with biochemical primary hyperparathyroidism and inconclusive results at US and (99m)Tc sestamibi scintigraphy were prospectively enrolled...
January 25, 2017: Radiology
https://www.readbyqxmd.com/read/28115177/prediction-of-lymph-node-parasite-load-from-clinical-data-in-dogs-with-leishmaniasis-an-application-of-radial-basis-artificial-neural-networks
#8
Rafaela Beatriz Pintor Torrecilha, Yuri Tani Utsunomiya, Luís Fábio da Silva Batista, Anelise Maria Bosco, Cáris Maroni Nunes, Paulo César Ciarlini, Márcia Dalastra Laurenti
Quantification of Leishmania infantum load via real-time quantitative polymerase chain reaction (qPCR) in lymph node aspirates is an accurate tool for diagnostics, surveillance and therapeutics follow-up in dogs with leishmaniasis. However, qPCR requires infrastructure and technical training that is not always available commercially or in public services. Here, we used a machine learning technique, namely Radial Basis Artificial Neural Network, to assess whether parasite load could be learned from clinical data (serological test, biochemical markers and physical signs)...
January 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28111337/worse-outcomes-of-patients-with-hfe-hemochromatosis-with-persistent-increases-in-transferrin-saturation-during-maintenance-therapy
#9
Edouard Bardou-Jacquet, Fabrice Lainé, Pascal Guggenbuhl, Jeff Morcet, Caroline Jézéquel, Dominique Guyader, Romain Moirand, Yves Deugnier
BACKGROUND & AIMS: Even if patients with hemochromatosis maintain low serum levels of ferritin, they still have an increased risk of general and joint symptoms, which reduce quality of life. This could be related to persistently increased transferrin saturation. We assessed whether duration of exposure to increased transferrin saturation during maintenance therapy is associated with more severe general and joint symptoms. METHODS: We performed a longitudinal cohort study of 266 individuals homozygous for the C282Y substitution in HFE, seen at a tertiary reference center in Rennes, France, and followed for 3 or more years after initial iron removal...
January 19, 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28107066/hematologic-plasma-biochemical-and-lipid-panel-reference-intervals-in-orange-winged-amazon-parrots-amazona-amazonica
#10
Claire Vergneau-Grosset, Tamsen Polley, Danielle Carrade Holt, William Vernau, Joanne Paul-Murphy
To establish reference intervals in orange-winged Amazon parrots ( Amazona amazonica ) for the complete blood count, plasma biochemical values, and lipid panel and to evaluate age- and sex-related variations, blood samples were obtained from 29 healthy juvenile and adult parrots. Concentrations of total protein, bile acids, phosphorus, total cholesterol, low-density-lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol were significantly higher in adult compared with juvenile birds, while uric acid concentration was significantly higher in juveniles...
December 2016: Journal of Avian Medicine and Surgery
https://www.readbyqxmd.com/read/28091907/evaluation-of-the-hepatoprotective-effect-of-l-ascorbate-1-2-hydroxyethyl-4-6-dimethyl-1-2-dihydropyrimidine-2-one-upon-exposure-to-carbon-tetrachloride
#11
A B Vyshtakalyuk, N G Nazarov, V V Zobov, S R Abdulkhakov, O A Minnekhanova, V E Semenov, I V Galyametdinova, G V Cherepnev, V S Reznik
Hepatoprotective properties of a new pyrimidine derivative - L-ascorbate 1-(2-hydroxyethyl)-4,6-dimethyl-1,2-dihydropyrimidine-2-one, synthesized on the basis Xymedon, were assessed in white rats exposed to CCl4. The compound under study administered prior to exposure to CCl4 reduced the deviation of biochemical parameters from reference values and severity of structural and morphological changes in liver, when compared to the control. Hepatoprotective properties of the studied compound were more pronounced than those of Xymedon...
January 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28088454/alpha-l-iduronidase-and-arylsulfatase-b-in-dried-blood-spots-on-filter-paper-biochemical-parameters-and-time-stability
#12
Ana Carolina Breier, Jaqueline Cé, Jamila Mezzalira, Vanessa V Daitx, Vitoria C Moraes, Mariana P S Goldim, Janice C Coelho
BACKGROUND: The goal of this study was to assess the biochemical parameters of the enzymes α-l-iduronidase (IDUA) and arylsulfatase B (ASB), which are deficient in mucopolysaccharidosis (MPS) I and VI, respectively, in dried blood spot (DBS) samples impregnated on filter paper. METHODS AND RESULTS: The optimal pH, Km, and Vmax of IDUA and ASB in DBS are hereby presented. After these analyses, the reference values for the activities of these enzymes in DBS with cutoff of 3...
January 11, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28087298/the-utility-of-sex-hormone-binding-globulin-in-hypogonadism-and-infertile-males
#13
Joshua Ring, Charles Welliver, Mike Parenteau, Stephen Markwell, Robert E Brannigan, Tobias S Köhler
PURPOSE: We sought to determine the role of sex hormone-binding globulin in patients with male infertility. MATERIALS AND METHODS: We retrospectively reviewed the records of 168 males seen at a fertility clinic from 2012 to 2014, to investigate the accuracy of total testosterone in the biochemical diagnosis of hypogonadism using calculated bioavailable testosterone as the reference value. We used multivariable analysis to assess sex hormone-binding globulin as an independent predictor of infertility...
January 10, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28080924/selected-plasma-biochemistry-analytes-of-healthy-captive-sulcata-african-spurred-tortoises-centrochelys-sulcata
#14
David Eshar, Ady Y Gancz, Nili Avni-Magen, Effi Wagshal, Lisa M Pohlman, Mark A Mitchell
The sulcata or African spurred tortoise (Centrochelys sulcata) is a large tortoise species that is commonly kept in zoologic collections and as a pet. The objectives of this study were to establish reference intervals for selected biochemical analytes in clinically healthy captive sulcata tortoises and to evaluate the impact of blood sampling site and sex. Blood samples were collected from 60 tortoises from either the dorsal coccygeal (tail) vein or the subcarapacial venous plexus based on their body size. The packed cell volume and refractometric total solids (TS) were determined...
December 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28040257/the-2016-update-of-the-international-study-group-isgps-definition-and-grading-of-postoperative-pancreatic-fistula-11-years-after
#15
REVIEW
Claudio Bassi, Giovanni Marchegiani, Christos Dervenis, Micheal Sarr, Mohammad Abu Hilal, Mustapha Adham, Peter Allen, Roland Andersson, Horacio J Asbun, Marc G Besselink, Kevin Conlon, Marco Del Chiaro, Massimo Falconi, Laureano Fernandez-Cruz, Carlos Fernandez-Del Castillo, Abe Fingerhut, Helmut Friess, Dirk J Gouma, Thilo Hackert, Jakob Izbicki, Keith D Lillemoe, John P Neoptolemos, Attila Olah, Richard Schulick, Shailesh V Shrikhande, Tadahiro Takada, Kyoichi Takaori, William Traverso, Charles R Vollmer, Christopher L Wolfgang, Charles J Yeo, Roberto Salvia, Marcus Buchler
BACKGROUND: In 2005, the International Study Group of Pancreatic Fistula developed a definition and grading of postoperative pancreatic fistula that has been accepted universally. Eleven years later, because postoperative pancreatic fistula remains one of the most relevant and harmful complications of pancreatic operation, the International Study Group of Pancreatic Fistula classification has become the gold standard in defining postoperative pancreatic fistula in clinical practice. The aim of the present report is to verify the value of the International Study Group of Pancreatic Fistula definition and grading of postoperative pancreatic fistula and to update the International Study Group of Pancreatic Fistula classification in light of recent evidence that has emerged, as well as to address the lingering controversies about the original definition and grading of postoperative pancreatic fistula...
March 2017: Surgery
https://www.readbyqxmd.com/read/28027666/distress-tolerance-treatment-for-weight-concern-in-smoking-cessation-among-women
#16
Erika Litvin Bloom, Rena R Wing, Christopher W Kahler, J Kevin Thompson, Sari Meltzer, Jacki Hecht, Haruka Minami, Lawrence H Price, Richard A Brown
Fear of gaining weight after quitting cigarette smoking is a major barrier to smoking cessation among women. Distress tolerance, which refers to one's ability and willingness to tolerate physical and emotional discomfort, predicts successful behavior change. Novel interventions rooted in Acceptance and Commitment Therapy (ACT) have emerged that aim to increase distress tolerance and engagement in values-oriented behavior. In this study, we developed a 9-week, group-based distress tolerance intervention for weight concern in smoking cessation among women (DT-W)...
December 1, 2016: Behavior Modification
https://www.readbyqxmd.com/read/28004439/prediction-of-fetal-growth-restriction-using-estimated-fetal-weight-versus-a-combined-screening-model-at-32-36-weeks-of-gestation
#17
Jezid Miranda, Merida Rodriguez-Lopez, Stefania Triunfo, Mikko Sairanen, Heikki Kouru, Miguel Parra-Saavedra, Francesca Crovetto, Francesc Figueras, Fatima Crispi, Eduard Gratacos
OBJECTIVES: To compare the performance of a third trimester screening based on estimated fetal weight (EFW) versus a combined model composed by maternal baseline characteristics, mean arterial pressure (MAP), ultrasound and biochemical markers for the prediction of smallness for gestational age (SGA) and late-onset fetal growth restriction (FGR). METHODS: Nested case-control study within a prospective cohort of 1,590 singleton gestations referred for third-trimester evaluation (32-36 weeks of gestation)...
December 22, 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28003718/histological-evaluation-of-non-alcoholic-fatty-liver-disease-and-its-correlation-with-different-noninvasive-scoring-systems-with-special-reference-to-fibrosis-a-single-center-experience
#18
Mitali M Rath, Manas K Panigrahi, Kaumudee Pattnaik, Pallavi Bhuyan, Sanjib K Kar, Bijay Misra, Debasis Misra, Chudamani Meher, Omprakash Agrawal, Jayshree Rath, Shivaram P Singh
BACKGROUND: Although liver biopsy remains the gold standard for the diagnosis of non-alcoholic fatty liver disease [NAFLD], many non-invasive markers of liver fibrosis have recently been proposed and assessed as surrogates of liver biopsy. AIMS AND OBJECTIVE: To evaluate the degree of liver fibrosis by different non-invasive fibrosis scoring systems and to compare each non-invasive fibrosis scoring system with histological fibrosis stage. MATERIALS AND METHODS: The study population consists of consecutive patients with biopsy proven NAFLD...
December 2016: Journal of Clinical and Experimental Hepatology
https://www.readbyqxmd.com/read/27989606/improving-diagnostic-recognition-of-primary-hyperparathyroidism-with-machine-learning
#19
Yash R Somnay, Mark Craven, Kelly L McCoy, Sally E Carty, Tracy S Wang, Caprice C Greenberg, David F Schneider
BACKGROUND: Parathyroidectomy offers the only cure for primary hyperparathyroidism, but today only 50% of primary hyperparathyroidism patients are referred for operation, in large part, because the condition is widely under-recognized. The diagnosis of primary hyperparathyroidism can be especially challenging with mild biochemical indices. Machine learning is a collection of methods in which computers build predictive algorithms based on labeled examples. With the aim of facilitating diagnosis, we tested the ability of machine learning to distinguish primary hyperparathyroidism from normal physiology using clinical and laboratory data...
December 15, 2016: Surgery
https://www.readbyqxmd.com/read/27957776/health-and-mineral-nutrition-status-of-yaks-in-southern-mustang-nepal
#20
Hajime Kumagai, Mitsumi Nakajima, Hiroki Anzai, Takashi Sakai, Kazato Oishi, Hiroyuki Hirooka, Manoj Kumar Shah
Biochemical values and mineral concentrations in blood plasma were investigated to evaluate the statuses of health and mineral nutrition among yaks in Mustang District, Nepal. In total, 118 plasma samples of female yaks collected in April and September/October of 2013-2015 were offered. Seventy-four percent of yaks showed lower plasma total-cholesterol concentrations than the lowest limit of reference range (100 mg/dL) and the values in spring (83.41 mg/dL) were lower (P < 0.05) than those in autumn (95...
December 12, 2016: Animal Science Journal, Nihon Chikusan Gakkaihō
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