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Alessia Nottegar, Fabrizio Tabbò, Claudio Luchini, Matteo Brunelli, Emilio Bria, Nicola Veronese, Antonio Santo, Sara Cingarlini, Eliana Gilioli, Chiara Ogliosi, Albino Eccher, Licia Montagna, Serena Pedron, Claudio Doglioni, Maria G Cangi, Giorgio Inghirami, Marco Chilosi
Pulmonary adenocarcinoma with enteric differentiation (PAED) is a rare subtype of lung adenocarcinoma recently recognized in the WHO classification. It is defined as an adenocarcinoma in which the enteric component exceeds 50% and have to show the expression of at least 1 immunohistochemical marker of enteric differentiation. Although the definition of this tumor type is very important, above all in the differential diagnosis between a primary lung tumor and a metastasis of colorectal adenocarcinoma, this cancer still lacks a distinctive immunohistochemical and molecular signature...
October 7, 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
Lawrence J Thomas, Laura Vitale, Thomas O'Neill, Ree Y Dolnick, Paul K Wallace, Hans Minderman, Lauren E Gergel, Eric M Forsberg, James M Boyer, James R Storey, Catherine D Pilsmaker, Russell A Hammond, Jenifer Widger, Karuna Sundarapandiyan, Andrea Crocker, Henry C Marsh, Tibor Keler
T cell immunoglobulin and mucin domain 1 (TIM-1) is a type I transmembrane protein that was originally described as kidney injury molecule 1 (KIM-1) due to its elevated expression in kidney and urine after renal injury. TIM-1 expression is also upregulated in several human cancers, most notably in renal and ovarian carcinomas, but has very restricted expression in healthy tissues thus representing a promising target for antibody-mediated therapy. To this end we have developed a fully human monoclonal IgG1 antibody specific for the extracellular domain of TIM-1...
September 26, 2016: Molecular Cancer Therapeutics
Harald Enzmann, Ralf Meyer, Karl Broich
The new European regulation for in vitro diagnostics (IVD) divides the certification of IVD including companion diagnostics (CDx) by notified bodies (NB) from the market authorization of medicines. With the new regulation, CDx will require conformity assessment which is expected to include clinical evidence by NB. This is a significant change from the current situation: until now most IVD have been certified based on their manufacturers' assessment. For one medicine assessed by the EMA, certification of several different CDx by different NB is possible...
September 23, 2016: Biomarkers in Medicine
Yunqi Bu, Xiao-Hua Zhou
Personalized medicine is an area of growing attention in medical research and practice. A market-ready companion diagnostic test (CDx) is used in personalized medicine for identifying the best treatment for an individual patient. Unfortunately, development of CDx may lag behind the development of the drug, and consequently we use a clinical trial assay (CTA) to enroll patients into the drug pivotal clinical trial instead. Thus, when CDx becomes available, a bridging study will be required to assess the drug efficacy in the CDx intended use (CDx IU) population...
September 20, 2016: Journal of Biopharmaceutical Statistics
Diana Murro Lin, Ji-Weon Park, Paolo Gattuso
Primary bladder signet-ring cell carcinoma (SRCC) is extremely rare and associated with an aggressive course. To our knowledge, we describe the first metastatic bladder SRCC identified in cerebrospinal fluid (CSF). A 68-year-old male with 1 year history of primary bladder SRCC with spinal metastasis presented with multiple falls and loss of consciousness. Brain imaging showed high signal in the frontoparietal sulci and superior cerebellum. CSF analysis was significant for increased leukocytes with monocyte predominance while protein and glucose values were within normal range...
September 15, 2016: Diagnostic Cytopathology
Xiang-Rong Zhao, Chao Gao, Yong Zhang, Lei Kong, Wei Qu, Jia Li, Yong-Sheng Gao, Yong-Hua Yu
BACKGROUND: The urachus is a vestigial tubular structure that connects the urinary bladder to the allantois during early embryonic development. Urachal carcinoma develops in the urachus, which is an embryological remnant of the urogenital sinus and allantois. The estimated annual incidence of urachal carcinoma in the general population is 0.01% of all cancers in adults. Moreover, urachal carcinoma accounts for 0.34% to 0.7% of all bladder carcinoma cases. And breast metastasis is extremely rarer...
August 2016: Medicine (Baltimore)
Antonio Marchetti, Maria Vittoria Pace, Alessia Di Lorito, Sara Canarecci, Lara Felicioni, Tommaso D'Antuono, Marcella Liberatore, Giampaolo Filice, Luigi Guetti, Felice Mucilli, Fiamma Buttitta
OBJECTIVES: Anaplastic Lymphoma Kinase (ALK) gene rearrangements have been described in 3-5% of lung adenocarcinomas (ADC) and their identification is essential to select patients for treatment with ALK tyrosine kinase inhibitors. For several years, fluorescent in situ hybridization (FISH) has been considered as the only validated diagnostic assay. Currently, alternative methods are commercially available as diagnostic tests. MATERIAL AND METHODS: A series of 217 ADC comprising 196 consecutive resected tumors and 21 ALK FISH-positive cases from an independent series of 702 ADC were investigated...
September 2016: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
Haiguang Zhao, Daniele Benetti, Lei Jin, Yufeng Zhou, Federico Rosei, Alberto Vomiero
Luminescent solar concentrators (LSCs) can potentially reduce the cost of solar cells by decreasing the photoactive area of the device and boosting the photoconversion efficiency (PCE). This study demonstrates the application of "giant" CdSe/Cdx Pb1-x S core/shell quantum dots (QDs) as light harvesters in high performance LSCs with over 1.15% PCE. Pb addition is critical to maximize PCE. First, this study synthesizes "giant" CdSe/Cdx Pb1-x S QDs with high quantum yield (40%), narrow size distribution (<10%), and stable photoluminescence in a wide temperature range (100-300 K)...
October 2016: Small
April A N Rose, Matthew G Annis, Dennie T Frederick, Marco Biondini, Zhifeng Dong, Lawrence N Kwong, Lynda Chin, Tibor Keler, Thomas Hawthorne, Ian R Watson, Keith T Flaherty, Peter M Siegel
PURPOSE: To determine if BRAF and/or MEK inhibitor-induced GPNMB expression renders melanomas sensitive to CDX-011, an antibody-drug conjugate targeting GPNMB. EXPERIMENTAL DESIGN: The TCGA melanoma dataset was interrogated for a panel of MITF-regulated melanosomal differentiation antigens, including GPNMB. BRAF mutant melanoma cell lines treated with BRAF or MEK inhibitors were assessed for GPNMB expression by RT-qPCR, immunoblot and FACs analyses. Transient siRNA-mediated knockdown approaches were used to determine if MITF is requirement for treatment-induced GPNMB upregulation...
August 11, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Johanna S M Mattsson, Hans Brunnström, Verena Jabs, Karolina Edlund, Karin Jirström, Stephanie Mindus, Linnéa la Fleur, Fredrik Pontén, Mats G Karlsson, Christina Karlsson, Hirsh Koyi, Eva Brandén, Johan Botling, Gisela Helenius, Patrick Micke, Maria A Svensson
BACKGROUND: Identification of targetable EML4-ALK fusion proteins has revolutionized the treatment of a minor subgroup of non-small cell lung cancer (NSCLC) patients. Although fluorescence in situ hybridization (FISH) is regarded as the gold standard for detection of ALK rearrangements, ALK immunohistochemistry (IHC) is often used as screening tool in clinical practice. In order to unbiasedly analyze the diagnostic impact of such a screening strategy, we compared ALK IHC with ALK FISH in three large representative Swedish NSCLC cohorts incorporating clinical parameters and gene expression data...
2016: BMC Cancer
Yogesh Kumar, Nidhi Shukla, Gatha Thacker, Isha Kapoor, Savita Lochab, Madam Lal Brahma Bhatt, Naibedya Chattopadhyay, Sabyasachi Sanyal, Arun Kumar Trivedi
: Drosophila caudal-related homeobox transcription factor 2 (CDX2) drives differentiation of the intestinal epithelium. Loss of CDX2 expression has been reported in several colorectal cancers and cancer cell lines with a potential inverse correlation between CDX2 levels and tumor stage. Ubiquitination of CDX2 leading to its down-regulation has been implicated in several studies; however, the E3 ubiquitin ligases involved in CDX2 ubiquitination have largely remained unknown. Here, it is mechanistically determined that the E3 ubiquitin ligase Fbw7 promotes CDX2 ubiquitination and degradation through two phosphodegron motifs present within CDX2 in a GSK3beta-dependent manner leading to its reduced expression and function in colon cancer cells...
July 28, 2016: Molecular Cancer Research: MCR
Rémy Lartia, Christopher K Jankowski, Sébastien Arseneau
A,D-substituted cyclodextrin (CDX) substituted on their primary rim side are ideal scaffolds for the macromolecular assembly and formation of templated structures. Their substitution can be achieved through various reactions. However, the use of the well-known Huisgen reaction in this context is under-reported. We present here results of the synthesis of model conjugates formed between CDX and representative peptides by click chemistry. Notably, bis-conjugation of peptides onto a unique scaffold promotes α-helix formation...
August 2016: Journal of Peptide Science: An Official Publication of the European Peptide Society
Yingjuan Lv, Qingbin Yao, Wenjiang Ma, Hua Liu, Jian Ji, Xiaorong Li
BACKGROUND: Vitamin D deficiency and vitamin D receptor gene polymorphisms are known to be significantly associated with high myopia. Whether this genetic variant may impact primary open-angle glaucoma is largely unknown. This study investigated whether vitamin D receptor gene polymorphisms are altered in primary open-angle glaucoma subjects carrying the risk allele, and whether vitamin D deficiency is an important factor in the development of glaucoma. METHODS: Seventy-three POAG patients and 71 age-matched controls from the Han population were enrolled...
2016: BMC Ophthalmology
Jana Brejchova, Miroslava Vosahlikova, Lenka Roubalova, Marco Parenti, Mario Mauri, Oleksandr Chernyavskiy, Petr Svoboda
Decrease of cholesterol level in plasma membrane of living HEK293 cells transiently expressing FLAG-δ-OR by β-cyclodextrin (β-CDX) resulted in a slight internalization of δ-OR. Massive internalization of δ-OR induced by specific agonist DADLE was diminished in cholesterol-depleted cells. These results suggest that agonist-induced internalization of δ-OR, which has been traditionally attributed exclusively to clathrin-mediated pathway, proceeds at least partially via membrane domains. Identification of internalized pools of FLAG-δ-OR by colocalization studies with proteins of Rab family indicated the decreased presence of receptors in early endosomes (Rab5), late endosomes and lysosomes (Rab7) and fast recycling vesicles (Rab4)...
August 2016: Journal of Bioenergetics and Biomembranes
Minhua Li, Jiangguo Wei, Xiaofei Yao, Cheng Wang
Purpose: Primary low-grade thyroid-like papillary adenocarcinomas are extremely rare neoplasms that generally originate in the nasopharynx. Here, we describe a novel case of a 15-year-old Chinese girl who was diagnosed with low-grade thyroid-like papillary adenocarcinoma, including a brief review of the literature to reveal the clinicopathological features of low-grade thyroid-like nasopharyngeal papillary adenocarcinoma. Materials and Methods: Immunohistochemistry was used to evaluate the expression of CKpan, CK7, TTF-1, vimentin, EMA, thyroglobulin, CD15, S100, P40, CK20, CDX-2, GFAP and Ki67...
July 4, 2016: Cancer Research and Treatment: Official Journal of Korean Cancer Association
Lu Lai, Si-Yu Sheng, Ping Mei, Yi Liu, Qing-Lian Guo
High-quality Cdx Zn1 - x Se and Cdx Zn1 - x Se/ZnS core/shell quantum dots (QDs) emitting in the violet-green spectral range have been successfully prepared using hydrothermal methods. The obtained aqueous Cdx Zn1 - x Se and Cdx Zn1 - x Se/ZnS QDs exhibit a tunable photoluminescence (PL) emission (from 433.5 nm to 501.2 nm) and a favorable narrow photoluminescence bandwidth [full width at half maximum (FWHM): 30-42 nm]. After coating with a ZnS shell, the quantum yield increases from 40.2% to 48...
June 30, 2016: Luminescence: the Journal of Biological and Chemical Luminescence
Dario Baratti, Shigeki Kusamura, Massimo Milione, Filippo Pietrantonio, Marta Caporale, Marcello Guaglio, Marcello Deraco
BACKGROUND: Pseudomyxoma peritonei (PMP) usually originates from appendiceal neoplasms and, less commonly, from extra-appendiceal lesions. To date, the clinical and therapeutic implications of extra-appendiceal origin are largely unknown. METHODS: A prospective database of 225 PMP patients uniformly treated by cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) was reviewed to identify cases with extra-appendiceal primaries. Histologically, negative appendix defined extra-appendiceal origin...
June 28, 2016: Annals of Surgical Oncology
Bita Geramizadeh, Ali Kashkooe, Seyed Ali Malekhosseini
BACKGROUND: Gastrointestinal neuroendocrine tumors (GI-NETs) are potentially malignant tumors, and their most common location of metastasis is the liver. OBJECTIVES: In this report, we will describe our experience with some clinical and pathologic findings of hepatic metastasis in a group of cases of GI-NETs at the largest referral center of GI and liver diseases in south Iran. MATERIALS AND METHODS: In this four-year study (2011 - 2014), all GI and liver NETs were extracted from the pathology files of hospitals affiliated with Shiraz University of Medical Sciences...
May 2016: Hepatitis Monthly
Jiajun Wang, Jie Meng, Qunxiang Li, Jinlong Yang
Recently, various single-layer materials have been explored as desirable photocatalyts for water splitting. In this work, based on extensive density functional theory calculations, we examine the geometric, electronic, optical, and potential photocatalytic properties of single-layer cadmium chalcogenides (CdX sheets, X = S, Se, and Te), which are cleaved from the (001) plane of the bulk wurtzite structure. The predicted formation energies have relatively low values and a suitable substrate (i.e. graphene) that can effectively stabilize CdX sheets, which imply that the fabrication and application of CdX sheets are highly possible in experiments...
June 22, 2016: Physical Chemistry Chemical Physics: PCCP
Raghidah Wagia, Ilya Strashnov, Michael W Anderson, Martin P Attfield
Identifying the form and role of the chemical species that traverse the stages of crystallization is critical to understanding the formation process of coordination polymers. Herein, we report the combined use of in situ atomic force microscopy and mass spectrometry to identify preformed, complex, cadmium 2-ethylimidazole containing solution species in the growth solution of the cadmium 2-ethylimidazolate metal-organic framework CdIF-4, and show that they are critical in the surface nucleation for the crystal growth of this material...
July 25, 2016: Angewandte Chemie
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