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https://www.readbyqxmd.com/read/28339719/the-pepper-wpp-domain-protein-cawdp1-acts-as-a-novel-negative-regulator-of-drought-stress-via-aba-signaling
#1
Chanmi Park, Chae Woo Lim, Woonhee Baek, Jung-Hyun Kim, Sohee Lim, Sang Hyon Kim, Kyung-Nam Kim, Sung Chul Lee
Plants are constantly challenged by various environmental stresses, including high salinity and drought, and they have evolved defense mechanisms to counteract the deleterious effects of these stresses. The plant hormone abscisic acid (ABA) regulates plant growth and developmental processes and mediates abiotic stress responses. Here, we report the identification and characterization of a novel CaWDP1 (Capsicum annuumWPP Domain Protein 1) protein. The expression of CaWDP1 in pepper leaves was induced by ABA, drought, and NaCl treatments, suggesting its role in the abiotic stress response...
February 22, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28330555/deciphering-the-role-of-the-phenylpropanoid-metabolism-in-the-tolerance-of-capsicum-annuum-l-to-verticillium-dahliae-kleb
#2
Marta Novo, Cristina Silvar, Fuencisla Merino, Teresa Martínez-Cortés, Fachuang Lu, John Ralph, Federico Pomar
Verticillium dahliae is an economically relevant soilborne pathogen that causes vascular wilt in several crops, including pepper (Capsicum annuum). Fungal infection is usually visualized as a vascular browning, likely due to the onset of phenylpropanoid metabolism, which also seems to play a crucial role in the tolerance of some pepper varieties. In the current work, the potential function of distinct phenylpropanoid derivatives (suberin, lignin and phenolic compounds) in the pepper tolerance response against V...
May 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28321795/phytase-production-by-aspergillus-niger-ncim-563-for-a-novel-application-to-degrade-organophosphorus-pesticides
#3
Parin C Shah, V Ravi Kumar, Syed G Dastager, Jayant M Khire
The production of phytase using Aspergillus niger NCIM 563 under submerged fermentation conditions was studied using protein rich chickpea flour as substrate. Employing a hybrid statistical media optimization strategy of Plackett-Burman and Box-Behnken experimental designs in shake-flasks gave an increased phytase activity from an initial 66 IU/mL in 216 h to 160 IU/mL in a reduced time of 132 h. Productivity, thus increased by 3.97 times from 7.3 to 29 IU/mL/day. Using the optimized media, the production was successfully scaled-up further and improved up to 164 IU/mL in 96 h by studies carried out employing 2 and 10-L fermenters...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28314149/cytokinin-response-in-pepper-plants-capsicum-annuum-l-exposed-to-silver-nanoparticles
#4
Tomislav Vinković, Ondrej Novák, Miroslav Strnad, Walter Goessler, Darija Domazet Jurašin, Nada Parađiković, Ivana Vinković Vrček
The increasing development of different nanomaterials, such as silver nanoparticles (AgNPs), and their practical use in agriculture and biotechnology has created a strong need for elucidations of biological effects and risk assessments of AgNPs in plants. This study was aimed to investigate AgNPs effects on metal uptake and their biodistribution in pepper plants as well as on morphological parameters and hormonal responses of the isoprenoid cytokinin (CK) family. In addition, the comparison of effects silver form, nanoparticles vs...
March 14, 2017: Environmental Research
https://www.readbyqxmd.com/read/28300025/evaluation-of-matrix-effects-in-multiresidue-analysis-of-pesticide-residues-in-vegetables-and-spices-by-lc-ms-ms
#5
Suchi Chawla, Hemlatta K Patel, Hetal N Gor, Kiran M Vaghela, Priti P Solanki, Paresh G Shah
The study was conducted to investigate matrix interferences using QuEChERS sample preparation to understand whether thedilution of matrix and/or the grouping of commodities can eliminate the need for selective individual matrix-matched standards in LC with tandem MS (MS/MS) analysis, and whether the calibration graph based on only one matrix can be used for quantification in the other matrixes. Matrix effects (MEs) were studied by comparing the slopes of calibration curves of the matrix-matched standards (diluted with mobile phase) vis-à-vis the solvent-based standards...
March 16, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28296187/lethal-and-sublethal-effects-of-pesticides-in-the-management-of-polyphagotarsonemus-latus-banks-acari-tarsonemidae-on-capsicum-annuum-l
#6
Mariana O Breda, José V Oliveira, Alberto B Esteves Filho, Douglas R S Barbosa, Andrezo A Santos
BACKGROUND: The evaluation of lethal and sublethal effects are of great importance for a complete assessment of the total impact of chemical compounds upon pest populations and the development of management strategies. At this study, the lethal and sublethal effects of different synthetic and botanical products were evaluated upon the broad mite, Polyphagotarsonemus latus (Banks), a major pest of Capsicum annuum L and other crops. RESULTS: Abamectin presented the highest lethal effect to P...
March 15, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28293725/a-nano-au-c-mwcnt-based-label-free-amperometric-immunosensor-for-the-detection-of-capsicum-chlorosis-virus-in-bell-pepper
#7
Anshul Sharma, Ankur Kaushal, Saurabh Kulshrestha
Accurate and on time diagnosis of plant viruses is an essential prerequisite for efficient control in field conditions. A number of diagnostic methods have been reported with the required level of sensitivity. Here, we propose a label free immunosensor for efficient and sensitive detection of capsicum chlorosis virus (CaCV) in bell pepper. Antigen was immobilized over the surface of gold nanoparticle/multi-walled carbon nanotube (Nano-Au/C-MWCNT) screen printed electrodes using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/N-hydroxysuccinimide (NHS) cross linking chemistry followed by interaction with groundnut bud necrosis virus (GBNV)/CaCV specific polyclonal antibody...
March 14, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28283949/impact-of-chemical-organic-and-bio-fertilizers-application-on-bell-pepper-capsicum-annuum-l-and-biological-parameters-of-myzus-persicae-sulzer-hem-aphididae
#8
M Mardani-Talaee, J Razmjou, G Nouri-Ganbalani, M Hassanpour, B Naseri
Myzus persicae (Sulzer) is a polyphagous aphid that causes chlorosis, necrosis, stunting, and reduce growth rate of the host plants. In this research, the effects of Zinc sulfate and vermicompost (30%), Bacillus subtilis, Pseudomonas fluorescens, Glomus intraradices, G. intraradices × B. subtilis, and G. intraradices × P. fluorescens compared to control was investigated on the growth characters of Capsicum annuum L. and biological parameters of M. persicae. Different fertilizers caused a significant effect on growth characters of C...
March 11, 2017: Neotropical Entomology
https://www.readbyqxmd.com/read/28281078/amelioration-of-boron-toxicity-in-sweet-pepper-as-affected-by-calcium-management-under-an-elevated-co2-concentration
#9
María Carmen Piñero, Margarita Pérez-Jiménez, Josefa López-Marín, Francisco M Del Amor
We investigated B tolerance in sweet pepper plants (Capsicum annuun L.) under an elevated CO2 concentration, combined with the application of calcium as a nutrient management amelioration technique. The data show that high B affected the roots more than the aerial parts, since there was an increase in the shoot/root ratio, when plants were grown with high B levels; however, the impact was lessened when the plants were grown at elevated CO2, since the root FW reduction caused by excess B was less marked at the high CO2 concentration (30...
March 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28252171/characterizing-and-marker-assisting-a-novel-chili-pepper-capsicum-annuum-l-yellow-bud-mutant-with-cytoplasmic-male-sterility
#10
G S Sun, Z L Dai, P W Bosland, Q Wang, C Q Sun, Z C Zhang, Z H Ma
Cytoplasmic male sterility (CMS) in pepper is a better way to produce hybrid seeds compared to manual production. We used the two sequence characterized amplified region (SCAR) markers (CRF-SCAR and CMS-SCAR130) in CMS pepper, to identify the genotype. We assembled two CMS yellow bud mutants (YBM; YBM12-A and YBM12-B). This mutation in leaf color is controlled by a single dominant nuclear gene. The aim was to create a new hybrid seed production method that reduces the costs and increases F1 hybrid seed purity...
February 23, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28231158/irradiation-maintains-functional-components-of-dry-hot-peppers-capsicum-annuum-l-under-ambient-storage
#11
Qumer Iqbal, Muhammad Amjad, Muhammad Rafique Asi, Aamir Nawaz, Samiya Mahmood Khan, Agustin Ariño, Tanveer Ahmad
Hot peppers used as natural flavoring and coloring agents are usually irradiated in prepacked form for decontamination. The effects of gamma radiation on the stability of functional components such as capsaicinoids and antioxidant compounds (carotenoids, ascorbic acid and total phenolics) were investigated in hot peppers (Capsicum annuum). Whole dried peppers packed in polyethylene bags were gamma irradiated at 0 (control), 2, 4, and 6 kGy and subsequently stored at 25 °C for 90 days. The irradiation dose did not substantially affect the initial contents of capsaicinoids, ascorbic acid and total phenolics, though the concentration of carotenoids declined by 8% from the control (76...
September 12, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28220324/virus-surveys-of-capsicum-spp-in-the-republic-of-benin-reveal-the-prevalence-of-pepper-vein-yellows-virus-and-the-identification-of-a-previously-uncharacterised-polerovirus-species
#12
Leonard Afouda, Daouda Kone, Valerien Zinsou, Laurence Dossou, Lawrence Kenyon, Stephan Winter, Dennis Knierim
Surveys were conducted in 2014 and 2015 in Southern and Northern Benin, respectively, to identify the viruses infecting peppers (Capsicum spp.). The samples were screened by ELISA for cucumber mosaic virus (CMV), pepper veinal mottle virus (PVMV), potato virus Y (PVY) and tomato yellow leaf curl virus (TYLCV). A generic reverse transcription PCR (RT-PCR) was used to test for the presence of poleroviruses. ELISA tests confirmed the prevalence of all viruses, while the RT-PCR detected pepper vein yellows virus (PeVYV) which is reported for the first time in Benin...
February 20, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28207797/mutation-increasing-%C3%AE-carotene-concentrations-does-not-adversely-affect-concentrations-of-essential-mineral-elements-in-pepper-fruit
#13
Nasya B Tomlekova, Philip J White, Jacqueline A Thompson, Emil A Penchev, Stephan Nielen
Vitamin and mineral deficiencies are prevalent in human populations throughout the world. Vitamin A deficiency affects hundreds of millions of pre-school age children in low income countries. Fruits of pepper (Capsicum annuum L.) can be a major dietary source of precursors to Vitamin A biosynthesis, such as β-carotene. Recently, pepper breeding programs have introduced the orange-fruited (of) trait of the mutant variety Oranzheva kapiya, which is associated with high fruit β-carotene concentrations, to the mutant variety Albena...
2017: PloS One
https://www.readbyqxmd.com/read/28204897/characterization-of-tomato-apical-stunt-viroid-isolated-from-a-24-year-old-seed-lot-of-capsicum-annuum
#14
J Th J Verhoeven, H M S Koenraadt, M Westenberg, J W Roenhorst
Tomato apical stunt viroid (TASVd) has been identified in a 24-year old seed lot of Capsicum annuum produced in Taiwan. It is the first finding of TASVd in this plant species. The isolate could be discriminated from all reported isolates of TASVd based on its nucleotide sequence, which showed only 94.8% identity with the most related genotype of TASVd. This discrimination was substantiated by phylogenetic analysis. Inoculation of a RNA extract of contaminated seeds to healthy pepper plants showed that the infectivity of the viroid had remained over time...
February 15, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28184010/the-pepper-ring-type-e3-ligase-caairf1-regulates-the-aba-and-drought-signaling-via-caadip1-protein-phosphatase-degradation
#15
Chae Woo Lim, Woonhee Baek, Sung Chul Lee
Ubiquitin-mediated protein modification occurs at multiple steps of abscisic acid (ABA) signaling. Here, we sought proteins responsible for degradation of the pepper type 2C protein phosphatase CaADIP1 via the 26S proteasome system. We showed that the RING type E3 ligase CaAIRF1 (Capsicum annuum ADIP1 Interacting RING Finger Protein 1) interacts with and ubiquitinates CaADIP1. CaADIP1 degradation was slower in crude proteins from CaAIRF1-silenced peppers than in those from control plants. CaAIRF1-silenced pepper plants displayed reduced ABA sensitivity and decreased drought tolerance characterized by delayed stomatal closure and suppressed induction of ABA- and drought-responsive marker genes...
February 9, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28182679/comparative-host-selection-responses-of-specialist-helicoverpa-assulta-and-generalist-helicoverpa-armigera-moths-in-complex-plant-environments
#16
Wei-Zheng Li, Xiao-Hui Teng, Hong-Fei Zhang, Ting Liu, Qiong Wang, Guohui Yuan, Xian-Ru Guo
We tested the behavioral responses of ovipositing females and natal larvae of two sibling species, a generalist Helicoverpa armigera (Hübner) and a specialist Helicoverpa assulta (Guenée), to odor sources emitted from different combinations of six plant species (tobacco, Nicotiana tabacum; hot pepper, Capsicum annuum; tomato, Solanum esculentum; cotton, Gossypium hirsutum; peanut, Arachis hypogaea; maize, Zea mays). Under the conditions of plant materials versus corresponding controls, both stages of both species could find their corresponding host plants...
2017: PloS One
https://www.readbyqxmd.com/read/28176159/new-korean-isolates-of-pepper-mild-mottle-virus-pmmov-differ-in-symptom-severity-and-subcellular-localization-of-the-126%C3%A2-kda-protein
#17
Sang-Hyuk Han, Jong-Seo Park, Jae-Yeong Han, Jun-Su Gong, Chan-Hwan Park, Jung-Kyu Kim, Eun-Young Seo, Leslie L Domier, John Hammond, Hyoun-Sub Lim
Two isolates of Pepper mild mottle virus (PMMoV) were selected from a nationwide survey of pepper fields in South Korea in 2014 and 2015, in which Cucumber mosaic virus was also detected; the two PMMoV isolates, Sangcheong 47 (S-47, KX399390) and Jeongsong 76 (J-76, KX399389), share ~99% nucleotide and amino acid identity and are closely related to Japanese and Chinese isolates at the nucleotide level. Amino acid sequence comparisons revealed 99.73, 99.81, 98.44, and 100% identity in the ORF1, ORF2, MP, and CP, respectively, between S-47 and J-76...
February 7, 2017: Virus Genes
https://www.readbyqxmd.com/read/28161544/purification-and-characterization-of-peptides-from-capsicum-annuum-fruits-which-are-%C3%AE-amylase-inhibitors-and-exhibit-high-antimicrobial-activity-against-fungi-of-agronomic-importance
#18
Layrana de Azevedo Dos Santos, Gabriel Bonan Taveira, Suzanna de Fátima Ferreira Ribeiro, Lídia da Silva Pereira, André de Oliveira Carvalho, Rosana Rodrigues, Antônia Elenir Amâncio Oliveira, Olga Lima Tavares Machado, Jucélia da Silva Araújo, Ilka Maria Vasconcelos, Valdirene Moreira Gomes
Proteins extracted from Capsicum annuum L. fruits were initially subjected to reversed-phase chromatography on HPLC, resulting in eight peptide-rich fractions. All the fractions obtained were tested for their ability to inhibit porcine trypsin and amylase from both human saliva and from larval insect in vitro. All fractions were also tested for their ability to inhibit growth of the phytopathogenic fungi. Several fractions inhibited the activity of human salivary amylase and larval insect amylase, especially fraction Fa5...
February 2, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28155188/inducible-expression-of-bs2-r-gene-from-capsicum-chacoense-in-sweet-orange-citrus-sinensis-l-osbeck-confers-enhanced-resistance-to-citrus-canker-disease
#19
Lorena Noelia Sendín, Ingrid Georgina Orce, Rocío Liliana Gómez, Ramón Enrique, Carlos Froilán Grellet Bournonville, Aldo Sergio Noguera, Adrián Alberto Vojnov, María Rosa Marano, Atilio Pedro Castagnaro, María Paula Filippone
Transgenic expression of the pepper Bs2 gene confers resistance to Xanthomonas campestris pv. vesicatoria (Xcv) pathogenic strains which contain the avrBs2 avirulence gene in susceptible pepper and tomato varieties. The avrBs2 gene is highly conserved among members of the Xanthomonas genus, and the avrBs2 of Xcv shares 96% homology with the avrBs2 of Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker disease. A previous study showed that the transient expression of pepper Bs2 in lemon leaves reduced canker formation and induced plant defence mechanisms...
February 2, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28140774/microcystin-accumulation-and-potential-effects-on-antioxidant-capacity-of-leaves-and-fruits-of-capsicum-annuum
#20
Damjana Drobac, Nada Tokodi, Biljana Kiprovski, Djordje Malenčić, Tamara Važić, Sonja Nybom, Jussi Meriluoto, Zorica Svirčev
Surface water, often used for irrigation purposes, may sometimes be contaminated with blooming cyanobacteria and thereby may contain their potent and harmful toxins. Cyanotoxins adversely affect many terrestrial plants, and accumulate in plant tissues that are subsequently ingested by humans. Studies were undertaken to (1) examine the bioaccumulation of microcystins (MCs) in leaves and fruits of pepper Capsicum annuum and (2) examine the potential effects of MCs on antioxidant capacity of these organs. Plants were irrigated with water containing MCs for a period of 3 mo...
January 31, 2017: Journal of Toxicology and Environmental Health. Part A
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