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https://www.readbyqxmd.com/read/28714761/gamma-irradiation-of-medicinally-important-plants-and-the-enhancement-of-secondary-metabolite-production
#1
P Vivek Vardhan, Lata I Shukla
PURPOSE: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area of interest. We reviewed different types of secondary metabolites, their mode of synthesis and effect of γ-radiation on their yield for different plants, organs and in vitro cultures (callus, suspension, hairy root). Special effort has been made to review the biochemical mechanisms underlying the increase in secondary metabolites...
July 17, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28706523/identification-of-optimal-reference-genes-for-normalization-of-qpcr-analysis-during-pepper-fruit-development
#2
Yuan Cheng, Xin Pang, Hongjian Wan, Golam J Ahammed, Jiahong Yu, Zhuping Yao, Meiying Ruan, Qingjing Ye, Zhimiao Li, Rongqing Wang, Yuejian Yang, Guozhi Zhou
Due to its high sensitivity and reproducibility, quantitative real-time PCR (qPCR) is practiced as a useful research tool for targeted gene expression analysis. For qPCR operations, the normalization with suitable reference genes (RGs) is a crucial step that eventually determines the reliability of the obtained results. Although pepper is considered an ideal model plant for the study of non-climacteric fruit development, at present no specific RG have been developed or validated for the qPCR analyses of pepper fruit...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28702041/characterization-of-bip-genes-from-pepper-capsicum-annuum-l-and-the-role-of-cabip1-in-response-to-endoplasmic-reticulum-and-multiple-abiotic-stresses
#3
Hu Wang, Huanhuan Niu, Yufei Zhai, Minghui Lu
Adverse environmental conditions have a detrimental impact on crop growth and development, and cause protein denaturation or misfolding. The binding protein (BiP) plays an important protective role by alleviating endoplasmic reticulum (ER) stress induced by misfolded proteins. In this study, we characterized three BiP genes (CaBiP1, CaBiP2, and CaBiP3) in pepper, an economically important vegetable and spice species. The role of CaBiP1 in plant tolerance to ER stress and adverse environmental conditions (including heat, salinity, osmotic and drought stress) were investigated...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28702034/cardr1-an-rna-dependent-rna-polymerase-plays-a-positive-role-in-pepper-resistance-against-tmv
#4
Lei Qin, Ning Mo, Yang Zhang, Tayeb Muhammad, Guiye Zhao, Yan Zhang, Yan Liang
RNA silencing functions as a major natural antiviral defense mechanism in plants. RNA-dependent RNA polymerases (RDRs) that catalyze the synthesis of double-stranded RNAs, are considered as a fundamental element in RNA silencing pathways. In Arabidopsis thaliana, RDR1, 2 and 6 play important roles in anti-viral RNA silencing. Expression of RDR1 can be elevated following plant treatment with defense hormones and virus infection. RDR1 has been studied in several crop species, but not in pepper (Capsicum annuum L...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28692124/genetic-variability-of-a-brazilian-capsicum-frutescens-germplasm-collection-using-morphological-characteristics-and-ssr-markers
#5
S I C Carvalho, L B Bianchetti, C F Ragassi, C S C Ribeiro, F J B Reifschneider, G S C Buso, F G Faleiro
Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - SSR) molecular markers. Morphological characterization was carried out using 57 descriptors, and molecular characterization was based on 239 alleles from 24 microsatellite loci...
July 6, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28692116/variability-among-capsicum-baccatum-accessions-from-goi%C3%A3-s-brazil-assessed-by-morphological-traits-and-molecular-markers
#6
A L A Martinez, J S P Araújo, C F Ragassi, G S C Buso, F J B Reifschneider
Capsicum peppers are native to the Americas, with Brazil being a significant diversity center. Capsicum baccatum accessions at Instituto Federal (IF) Goiano represent a portion of the species genetic resources from central Brazil. We aimed to characterize a C. baccatum working collection comprising 27 accessions and 3 commercial cultivars using morphological traits and molecular markers to describe its genetic and morphological variability and verify the occurrence of duplicates. This set included 1 C. baccatum var...
July 6, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28691663/quantitative-trait-loci-in-pepper-control-the-effective-population-size-of-two-rna-viruses-at-inoculation
#7
Lucie Tamisier, Elsa Rousseau, Sebastien Barraillé, Ghislaine Nemouchi, Marion Szadkowski, Ludovic Mailleret, Frederic Grognard, Frederic Fabre, Benoit Moury, Alain Palloix
Infection of plants by viruses is a complex process involving several steps: inoculation into plant cells, replication in inoculated cells and plant colonization. The success of the different steps depends, in part, on the viral effective population size (Ne), defined as the number of individuals passing their genes to the next generation. During infection, the virus population will undergo bottlenecks, leading to drastic reductions in Ne and, potentially, to the loss of the fittest variants. Therefore, it is crucial to better understand how plants affect Ne...
July 8, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28686086/a-risk-assessment-model-for-bacterial-leaf-spot-bls-of-pepper-capsicum-annuum-l-caused-by-xanthomonas-euvesicatoria-based-on-concentrations-of-macronutrients-and-micronutrients-and-micronutrient-ratios
#8
Bhabesh Dutta, David B Langston, Xuelin Luo, Jeremy Kichler, Scott Carlson, Ron Gitaitis
The phytopathogenic bacterium Xanthomonas euvesicatoria causes bacterial leaf spot (BLS) of pepper and has a worldwide distribution. BLS is difficult to control and an integrated management strategy that incorporates crop rotation, use of clean seeds and clean plants, weed control, resistant varieties, and applications of bactericides, biocontrol agents, and systemic acquired resistance (SAR) inducers is generally recommended. However, even with that arsenal of weapons, BLS can still be responsible for severe losses under favorable environmental conditions...
July 7, 2017: Phytopathology
https://www.readbyqxmd.com/read/28671253/identification-of-colletotrichum-isolates-from-capsicum-chinense-in-amazon
#9
C V S de Oliveira, K S Matos, D M C de Albuquerque, R E Hanada, G F da Silva
Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular characterization, using RFLP-PCR, ERIC-PCR and ISSR, was carried out initially for screening of morphologically similar isolates...
June 29, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28664295/complete-genome-sequence-of-a-capsicum-chlorosis-virus-in-china-and-the-structural-variation-and-evolutionary-origin-of-its-s-rna-intergenic-region
#10
Ying Huang, Hao Hong, Xiao-Hui Zhao, Jia Li, Xiao-Rong Tao
The complete genome sequence of a Capsicum chlorosis virus from China (CaCV-Hainan) was determined. The tripartite genome of CaCV-Hainan consists of small (S), medium (M), and large (L) RNAs of 3629, 4859, and 8912 nucleotides (nt), respectively. The S and M RNAs contain intergenic regions (IGRs) of 1348 and 462 nt, respectively. Strikingly, sequence comparisons among CaCV isolates revealed that the S RNA IGR of CaCV-Hainan derived from the CaCV-Qld-3432 Australia isolate through deletion of two stretches of 25- and 325-nt sequences within the S RNA IGR of CaCV-Qld-3432...
June 29, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28649008/environmental-traditional-knowledge-in-a-natural-protected-area-as-the-basis-for-management-and-conservation-policies
#11
Juan Fernando Pío-León, Francisco Delgado-Vargas, Bernardo Murillo-Amador, José Luís León-de-la-Luz, Rito Vega-Aviña, Alejandra Nieto-Garibay, Miguel Córdoba-Matson, Alfredo Ortega-Rubio
Ethnobotany is an adequate tool to identify the most import wild edible plants used by local people and to develop strategies for its sustainable use and policy making. This research records the wild edible plants used by the inhabitants of the Sierra la Laguna Biosphere Reserve, in Mexico, and identified priority species for sustainable development. The employed quantitative approach included the application of a food-oriented index named Food Significance Index (FSI), which integrated cultural, agricultural and food parameters; moreover, it used multivariate analysis to identify priority edible species for use and/or conservation...
June 22, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28631818/inactivation-of-bacillus-cereus-spores-on-red-chili-peppers-using-a-combined-treatment-of-aqueous-chlorine-dioxide-and-hot-air-drying
#12
Songyi Kim, Huyong Lee, Jee-Hoon Ryu, Hoikyung Kim
The effect of a combined treatment using aqueous chlorine dioxide (ClO2 ) and hot-air drying to inactivate Bacillus cereus spores on red chili peppers was evaluated. Ten washed and dried pepper samples, each comprising half of a single pepper (Capsicum annuum L.), were inoculated with B. cereus spore suspension. The inoculated samples were washed with sodium hypochlorite (NaOCl; 50, 100, or 200 μg/mL) or ClO2 (50, 100, or 200 μg/mL) solution for 1 min and then air-dried (25 ± 1 °C, 47 ± 1% relative humidity), which was followed by drying with hot air at 55 °C for up to 48 h...
June 20, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28631815/expansion-of-sesquiterpene-biosynthetic-gene-clusters-in-pepper-confers-nonhost-resistance-to-the-irish-potato-famine-pathogen
#13
Hyun-Ah Lee, Sejun Kim, Seungill Kim, Doil Choi
Chemical barriers contribute to nonhost resistance, which is defined as the resistance of an entire plant species to nonadapted pathogen species. However, the molecular basis of metabolic defense in nonhost resistance remains elusive. Here, we report genetic evidence for the essential role of phytoalexin capsidiol in nonhost resistance of pepper (Capsicum spp.) to potato late blight Phytophthora infestans using transcriptome and genome analyses. Two different genes for capsidiol biosynthesis, 5-epi-aristolochene synthase (EAS) and 5-epi-aristolochene-1,3-dihydroxylase (EAH), belong to multigene families...
June 20, 2017: New Phytologist
https://www.readbyqxmd.com/read/28624494/antibacterial-activity-and-phospholipid-recognition-of-the-recombinant-defensin-j1-1-from-capsicum-genus
#14
Francisco Guillén-Chable, Iván Arenas-Sosa, Ignacio Islas-Flores, Gerardo Corzo, Cynthia Martinez-Liu, Georgina Estrada
The gene of the four disulfide-bridged defensin J1-1 from Capsicum was cloned into the expression vector pQE30 containing a 6His-tag as fusion protein. This construct was transfected into Origami strain of Escherichia coli and expressed after induction with isopropyl thiogalactoside (IPTG). The level of expression was 4 mg/L of culture medium, and the His-tagged recombinant defensin (HisXarJ1-1) was expressed exclusively into inclusion bodies. After solubilization, HisXarJ1-1 was purified by affinity and hydrophobic interaction chromatography...
June 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28612389/biochar-potential-in-intensive-cultivation-of-capsicum-anuum-l-sweet-pepper-crop-yield-and-plant-protection
#15
Abhay Kumar, Yigal Elad, Ludmila Tsechansky, Vikas Abrol, Beni Lew, Rivka Offenbach, Ellen R Graber
BACKGROUND: The influence of various biochars on crop yield and disease resistance of Capsicum anuum L. (sweet pepper) under modern, high-input, intensive net house cultivation was tested over the course of 2011 to 2014 in the Arava desert region of Israel. A pot experiment with Lactuca sativa L. (lettuce) grown in the absence of fertilizer employed the 3-year old field trial soils to determine if biochar treatments contributed to soil intrinsic fertility. RESULTS: Biochar amendments resulted in a significant increase in number and weight of pepper fruits over three years...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28588235/parasitic-nematode-meloidogyne-incognita-interactions-with-different-capsicum-annum-cultivars-reveal-the-chemical-constituents-modulating-root-herbivory
#16
Ruth Kihika, Lucy K Murungi, Danny Coyne, Margaret Ng'ang'a, Ahmed Hassanali, Peter E A Teal, Baldwyn Torto
Plant volatile signatures are often used as cues by herbivores to locate their preferred hosts. Here, we report on the volatile organic compounds used by the subterranean root-knot nematode (RKN) Meloidogyne incognita for host location. We compared responses of infective second stage juveniles (J2s) to root volatiles of three cultivars and one accession of the solanaceous plant, Capsicum annum against moist sand in dual choice assays. J2s were more attracted to the three cultivars than to the accession, relative to controls...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28584274/overexpression-of-pea-dna-helicase-45-pdh45-imparts-tolerance-to-multiple-abiotic-stresses-in-chili-capsicum-annuum-l
#17
Tagginahalli N Shivakumara, Rohini Sreevathsa, Prasanta K Dash, M S Sheshshayee, Pradeep K Papolu, Uma Rao, Narendra Tuteja, M UdayaKumar
Imparting tolerance to abiotic stresses is of global importance as they inflict significant yield losses in field as well as in vegetable crops. Transcriptional activators, including helicases are identified to play a pivotal role in stress mitigation. Helicases, also known as molecular motors, are involved in myriad cellular processes that impart intrinsic tolerance to abiotic stresses in plants. Our study demonstrates the potential of a Pea DNA Helicase 45 (PDH45), in combating multiple abiotic stresses in chili...
June 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28578966/new-findings-on-green-sweet-pepper-capsicum-annum-pectins-rhamnogalacturonan-and-type-i-and-ii-arabinogalactans
#18
Georgia Erdmann do Nascimento, Marcello Iacomini, Lucimara M C Cordeiro
Polysaccharides were extracted from sweet pepper (Capsicum annum) with hot water and named ANW (9% yield). Starch was precipitated by freeze-thaw treatment, while pectic polysaccharides (8% yield) remained soluble and consisted of GalA (67.0%), Rha (1.6%), Ara (6.4%), Xyl (0.3%), Gal (6.7%) and Glc (4.4%). A highly methoxylated homogalacturonan (HG, degree of methylesterification of 85% and degree of acetylation of 5%), and type I and type II arabinogalactans (AG-I and AG-II) were observed in NMR analyses. These were fractionated with Fehling's solution to give HG (5...
September 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28573647/brazilian-capsicum-peppers-capsaicinoids-content-and-antioxidant-activity
#19
Stanislau Bogusz, Silvia H Libardi, Fernanda F G Dias, Janclei P Coutinho, Vivian C Bochi, Daniele Rodrigues, Arlete M T Melo, Helena T Godoy
BACKGROUND: Capsicum peppers are known as a source of capsaicinoids, phenolic compounds, and antioxidants. Brazilian Capsicum peppers are important spices use in foods worldwide. However, little information is available on the chemical composition and antioxidant activity of these peppers. RESULTS: Capsaicin, dihydrocapsaicin, total phenolic compounds, and antioxidant activity were investigated in the extracts of three Brazilian peppers: Capsicum frutescens, C. chinense and C...
June 2, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28572963/antibacterial-activity-of-jalape%C3%A3-o-pepper-capsicum-annuum-var-annuum-extract-fractions-against-select-foodborne-pathogens
#20
Karleigh Bacon, Renee Boyer, Cynthia Denbow, Sean O'Keefe, Andrew Neilson, Robert Williams
Capsicum annuum fruits have been investigated for antimicrobial activity in a number of studies. Capsaicin or other cinnamic acid pathway intermediates are often suggested to be the antimicrobial component, however there are conflicting results. No research has specifically fractionated jalapeño pepper (Capsicum annuum var. annuum) extract to isolate and identify compound(s) responsible for inhibition. In this study, fractions were collected from jalapeño pepper extracts using reverse-phase HPLC and tested for antibacterial activity using the disk diffusion method...
May 2017: Food Science & Nutrition
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