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Padakandla Nagasukeerthi, A Mooventhan, N K Manjunath
BACKGROUND: Type 2 diabetes mellitus (T2DM) is a major global health problem. Though various studies have reported the beneficial effect of Yoga in patient with T2DM, there is a lack of study in combination with bell pepper and yoga. Hence, the present study aims at evaluating short-term effect of add on bell pepper juice with integrated approach of yoga therapy (IAYT) on blood glucose levels and cardiovascular variables in patients with T2DM. MATERIALS AND METHODS: Fifty T2DM subjects with the age varied from 34 to 69-years were recruited and randomly divided into either study group or control group...
October 2017: Complementary Therapies in Medicine
Eunji Hong, Chae Woo Lim, Sang-Wook Han, Sung Chul Lee
Abscisic acid (ABA) is a plant hormone that plays a critical role in the response to environmental stress conditions, especially regulation of the stomatal aperture under water-deficit conditions. The signal transduction occurring during the stress response is initiated by transcription of defense-related genes. Here, we isolated the pepper ethylene-responsive transcription factor CaAIEF1 (Capsicum annuum ABA Induced ERF 1). The CaAIEF1 gene was significantly induced after exposure to ABA, drought, and high salinity...
2017: Frontiers in Plant Science
Rahul S Tanpure, Ranjit S Barbole, Vishal V Dawkar, Yashashree A Waichal, Rakesh S Joshi, Ashok P Giri, Vidya S Gupta
Plant proteinase inhibitors (PIs) are plant defense proteins and considered as potential candidates for engineering plant resistances against herbivores. Capsicum annuum proteinase inhibitor (CanPI7) is a multi-domain potato type II inhibitor (Pin-II) containing four inhibitory repeat domains (IRD), which target major classes of digestive enzymes in the gut of Helicoverpa armigera larvae. Stable integration and expression of the transgene in T1 transgenic generation, were confirmed by established molecular techniques...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Tea Stipcovich, Gerardo F Barbero, Marta Ferreiro-González, Miguel Palma, Carmelo G Barroso
A rapid high-performance liquid chromatography method with a C18 reverse-phase fused-core column has been developed for the determination and quantification of the main capsaicinoids (nornordihydrocapsaicin, nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin and homodihydrocapsaicin) present in Naga Jolokia peppers. A fused-core Kinetex™ C18 column (50×2.1mm i.d.; 2.6μm) was used for the analysis. The chromatographic separation was obtained with a gradient method in which the mobile phase was water (0...
January 15, 2018: Food Chemistry
M F Lima, S I C Carvalho, C F Ragassi, L B Bianchetti, F G Faleiro, F J B Reifschneider
Germplasm banks are essential as sources of genetic variability for plant breeding programs. To characterize a Brazilian Capsicum frutescens collection, 21 malagueta and 5 Tabasco hot pepper accessions were evaluated under field and greenhouse conditions regarding morphological and molecular traits, as well as resistance to viruses. Morphological characterization was performed using 53 IPGRI (International Plant Genetic Resources Institute) descriptors, 15 vegetative, 13 inflorescence, 22 fruit, and 3 seed...
August 31, 2017: Genetics and Molecular Research: GMR
R Ananthan, K Subhash, T Longvah
The world hottest Naga king chilli is cultivated and consumed in Northeast India. Capsaicinoids, amino acids and fatty acids were studied in fruit and fruit components of Naga king chilli. Capsaicinoid content was increased in each ripening stage and maximum level was observed at red color fruits. Total protein and fat content of placenta was 19.41 and 20.36% respectively. Capsaicinoids of placenta (7.35±2.241%) was higher followed by seed (3.83±1.358%) and pericarp (2.91±0.667%). Similarly, essential amino acid content was also higher in placenta compared to other components...
January 1, 2018: Food Chemistry
I M Al-Shahwan, O A Abdalla, M A Al-Saleh, M A Amer
A total of 1368 symptomatic plant samples showing different virus-like symptoms such as mottling, chlorosis, mosaic, yellow mosaic, vein clearing and stunting were collected from alfalfa, weed and cultivated plant species growing in vicinity of alfalfa fields in five principal regions of alfalfa production in Saudi Arabia. DAS-ELISA test indicated occurrence of 11 different viruses in these samples, 10 of which were detected for the first time in Saudi Arabia. Eighty percent of the alfalfa samples and 97.5% of the weed and cultivated plants samples were found to be infected with one or more of these viruses...
September 2017: Saudi Journal of Biological Sciences
Soumya Rao, Madhusudan R Nandineni
Colletotrichum truncatum, a major fungal phytopathogen, causes the anthracnose disease on an economically important spice crop chilli (Capsicum annuum), resulting in huge economic losses in tropical and sub-tropical countries. It follows a subcuticular intramural infection strategy on chilli with a short, asymptomatic, endophytic phase, which contrasts with the intracellular hemibiotrophic lifestyle adopted by most of the Colletotrichum species. However, little is known about the molecular determinants and the mechanism of pathogenicity in this fungus...
2017: PloS One
Javed Yakoob, Zaigham Abbas, Malik Hassan Mehmood, Kanwal Tariq, Saima Azhar Saleem, Safia Awan, Abdul Malik, Saeed Hamid, Rustam Khan, Wasim Jafri
OBJECTIVE: Helicobacter pylori is a Gram-negative organism. Its outer membrane protein Q (HopQ) mediates host-pathogen interactions; HopQ genotypes 1 and 2 are found associating with gastroduodenal pathologies. The authors measured the anti-adhesion effects of the extracts of Abelmoschus esculentus, Zingiber officinale, Trachyspermum ammi, Glycyrrhiza glabra, Curcuma longa and Capsicum annum against HopQ genotypes and H. pylori cytotoxin-associated gene A (CagA). METHODS: DNA was extracted by polymerase chain reaction of the HopQ genotypes (i...
September 2017: Journal of Integrative Medicine
Jing Li, Jianming Xie, Jihua Yu, Jian Lv, Junfeng Zhang, Xiaolong Wang, Cheng Wang, Chaonan Tang, Yingchun Zhang, Mohammed Mujitaba Dawuda, Daiqiang Zhu, Guoli Ma
Carotenoids are considered to be crucial elements in many fields and, furthermore, the significant factor in pepper leaves under low light and chilling temperature. However, little literature focused on the method to determinate and extract the contents of carotenoid compositions in pepper leaves. Therefore, a time-saving and highly sensitive reversed-phase high-performance liquid chromatography method for separation and quantification of 10 carotenoids was developed, and an optimized technological process for carotenoid composition extraction in pepper leaves was established for the first time...
September 8, 2017: Journal of Agricultural and Food Chemistry
Shaoliang Mou, Zhiqin Liu, Feng Gao, Sheng Yang, Meixia Su, Lei Shen, Yang Wu, Shuilin He
Homeodomain-leucine zipper class I (HD-Zip I) transcription factors (TFs) have been functionally characterized in plant responses to abiotic stresses, but their roles in plant immunity are poorly understood. Here, a HD-Zip I gene, CaHZ27, was isolated from pepper (Capsicum annum) and characterized for its role in pepper immunity. Quantitative real-time PCR showed that CaHDZ27 was transcriptionally induced by Ralstonia solanacearum inoculation and exogenous application of methyl jasmonate (MeJA), salicylic acid (SA), or ethephon (ETH)...
August 25, 2017: Molecular Plant-microbe Interactions: MPMI
R Sandoval-Oliveros, L Guevara-Olvera, J P Beltrán, C Gómez-Mena, G Acosta-García
Pepper (Capsicum annuum L.) is an important horticultural crop in many regions of the world. The final shape and size of the fruit are known to be determined at a very early step of flower development. During flower development hormonal treatments using gibberellins seem to promote growth resulting in higher yield and fruit quality. However, the morphological changes that occur in the pepper flowers after these treatments are largely unknown. In the present study, we provide a description of floral development landmarks of jalapeño chili pepper (cultivar Huichol), divided in nine representative stages from its initiation until the opening of the bud...
August 24, 2017: Plant Reproduction
Yuan Jin, Richard E Goodman, Afua O Tetteh, Mei Lu, Leena Tripathi
Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap) or/and the plant ferredoxin-like protein (Pflp) gene(s) from sweet pepper (Capsicum annuum). Several of these GM banana events showed 100% resistance to BXW disease under field conditions in Uganda. The current study evaluated the potential allergenicity and toxicity of the expressed proteins HRAP and PFLP based on evaluation of published information on the history of safe use of the natural source of the proteins as well as established bioinformatics sequence comparison methods to known allergens (www...
August 19, 2017: Food and Chemical Toxicology
A C Maracahipes, J W S Correa, P E Teodoro, K L Araújo, M A A Barelli, L G Neves
Anthracnose is among the major diseases of the Capsicum culture. It is caused by different species of the genus Colletotrichum, which may result in major damages to the cultivation of this genus. Studies aiming to search for cultivars resistant to diseases are essential to reduce financial and agricultural losses. The objective of this study was to evaluate the correlation between the variables analyzed to select Capsicum genotypes resistant to the fungus Colletotrichum gloeosporioides. The experimental design was completely randomized blocks with three replications, 88 treatments, four ripe fruits, and four unripe fruits per replication...
August 17, 2017: Genetics and Molecular Research: GMR
Magaji G Usman, Mohd Y Rafii, Mohammad Y Martini, Oladosu A Yusuff, Mohd R Ismail, Gous Miah
Backcrossing together with simple sequence repeat marker strategy was adopted to improve popular Malaysian chilli Kulai (Capsicum annuum L.) for heat tolerance. The use of molecular markers in backcross breeding and selection contributes significantly to overcoming the main drawbacks such as increase linkage drag and time consumption, in the ancient manual breeding approach (conventional), and speeds up the genome recovery of the recurrent parent. The strategy was adopted to introgress heat shock protein gene(s) from AVPP0702 (C...
August 15, 2017: Cell Stress & Chaperones
Gloria Aderonke Otunola, Anthony Jide Afolayan, Emmanuel Olusegun Ajayi, Samuel Wale Odeyemi
BACKGROUND: Herbal drug delivery is limited by poor solubility and bioavailability which can be overcome with suitable nanomaterials that will enhance their pharmacokinetics and performance. OBJECTIVE: This study aimed to analyze the synthesis, characterization, and biological activities of silver nanoparticles (AgNPs) from three spices. MATERIALS AND METHODS: AgNPs were prepared using 0.1 M silver nitrate and aqueous extracts of Allium sativum L...
July 2017: Pharmacognosy Magazine
Samia Azabou, Fadia Ben Taheur, Mourad Jridi, Mohamed Bouaziz, Moncef Nasri
The chemical composition and the antioxidant properties of Capsicum annum discarded seeds from processing industry with their corresponding extracted oil were investigated. C. annum seeds had high levels of crude proteins (18.30%), crude oil (11.04%), and dietary fibers (60.96%). The lipophilic fraction of C. annum seeds showed higher radical scavenging activity compared to their hydrophilic fraction, while this latter exhibited the highest reducing power. The results of fatty acid composition showed that fatty acids present in C...
August 9, 2017: Environmental Science and Pollution Research International
Mi-Seon Hahm, Jin-Soo Son, Ye-Ji Hwang, Duk-Ki Kwon, Sa-Youl Ghim
In the present study, we demonstrate that the growth of salt-stressed pepper plants is improved by inoculation with plant growth-promoting rhizobacteria (PGPR). Three PGPR strains (Microbacterium oleivorans KNUC7074, Brevibacterium iodinum KNUC7183, and Rhizobium massiliae KNUC7586) were isolated from the rhizosphere of pepper plants growing in saline soil, and pepper plants inoculated with these PGPR strains exhibited significantly greater plant height, fresh weight, dry weight, and total chlorophyll content than non-inoculated plants...
August 8, 2017: Journal of Microbiology and Biotechnology
Naiying Wu, Wei Gao, Yunhe Lian, Jingjing Du, Xiaowei Tie
Occurrence of Rhodamine B (RhB) contamination in paprika caused by agricultural materials during the vegetation process has been reported. It may transfer during the process of active compounds extraction, and eventually exist in final products. Herein, the re-distribution of RhB during the extraction process was assessed in terms of RhB contents, as well as mass, color value and capsaicinoids yield of each process. Results revealed that natural RhB contamination at 0.55-1.11µg/kg originated from raw paprika fruit then transferred with the extraction proceeded...
December 15, 2017: Food Chemistry
Zhiqin Liu, Lanping Shi, Sheng Yang, Youquan Lin, Yahong Weng, Xia Li, Ansar Hussain, Ali Noman, Shuilin He
Despite the involvement of many members of the chitinase family in plant immunity, the precise functions of the majority of the members remain poorly understood. Herein, the gene ChiIV3 in Capsicum annuum encoding a chitinase protein containing a chitin binding domain and targeting to the plasma membrane was found to be induced by Phytophthora capsici inoculation (PCI) and applied chitin treatment. Besides its direct inhibitory effect on growth of Phytophthora capsici (P. capsici), ChiIV3 was also found by virus-induced gene silencing (VIGS) and transient overexpression (TOE) in pepper plants to act as a positive regulator of plant cell death and in triggering defense signaling and upregulation of PR (pathogenesis related) genes against PCI...
August 1, 2017: International Journal of Molecular Sciences
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