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https://www.readbyqxmd.com/read/28812232/introgression-of-heat-shock-protein-hsp70-and-shsp-genes-into-the-malaysian-elite-chilli-variety-kulai-capsicum-annuum-l-through-the-application-of-marker-assisted-backcrossing-mab
#1
Magaji G Usman, Mohd Y Rafii, Mohammad Y Martini, Oladosu A Yusuff, Mohd R Ismail, Gous Miah
Backcrossing together with simple sequence repeat marker strategy was adopted to improve popular Malaysian chilli Kulai (Capsicum annuum L.) for heat tolerance. The use of molecular markers in backcross breeding and selection contributes significantly to overcoming the main drawbacks such as increase linkage drag and time consumption, in the ancient manual breeding approach (conventional), and speeds up the genome recovery of the recurrent parent. The strategy was adopted to introgress heat shock protein gene(s) from AVPP0702 (C...
August 15, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28808381/characterization-antibacterial-and-antioxidant-properties-of-silver-nanoparticles-synthesized-from-aqueous-extracts-of-allium-sativum-zingiber-officinale-and-capsicum-frutescens
#2
Gloria Aderonke Otunola, Anthony Jide Afolayan, Emmanuel Olusegun Ajayi, Samuel Wale Odeyemi
BACKGROUND: Herbal drug delivery is limited by poor solubility and bioavailability which can be overcome with suitable nanomaterials that will enhance their pharmacokinetics and performance. OBJECTIVE: This study aimed to analyze the synthesis, characterization, and biological activities of silver nanoparticles (AgNPs) from three spices. MATERIALS AND METHODS: AgNPs were prepared using 0.1 M silver nitrate and aqueous extracts of Allium sativum L...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28795322/discarded-seeds-from-red-pepper-capsicum-annum-processing-industry-as-a-sustainable-source-of-high-added-value-compounds-and-edible-oil
#3
Samia Azabou, Fadia Ben Taheur, Mourad Jridi, Mohamed Bouaziz, Moncef Nasri
The chemical composition and the antioxidant properties of Capsicum annum discarded seeds from processing industry with their corresponding extracted oil were investigated. C. annum seeds had high levels of crude proteins (18.30%), crude oil (11.04%), and dietary fibers (60.96%). The lipophilic fraction of C. annum seeds showed higher radical scavenging activity compared to their hydrophilic fraction, while this latter exhibited the highest reducing power. The results of fatty acid composition showed that fatty acids present in C...
August 9, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28783895/alleviation-of-salt-stress-in-pepper-capsicum-annum-l-plants-by-plant-growth-promoting-rhizobacteria
#4
Mi-Seon Hahm, Jin-Soo Son, Ye-Ji Hwang, Duk-Ki Kwon, Sa-Youl Ghim
In the present study, we demonstrate that the growth of salt-stressed pepper plants is improved by inoculation with plant growth-promoting rhizobacteria (PGPR). Three PGPR strains (Microbacterium oleivorans KNUC7074, Brevibacterium iodinum KNUC7183, and Rhizobium massiliae KNUC7586) were isolated from the rhizosphere of pepper plants growing in saline soil, and pepper plants inoculated with these PGPR strains exhibited significantly greater plant height, fresh weight, dry weight, and total chlorophyll content than non-inoculated plants...
August 8, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28764068/the-transfer-of-natural-rhodamine-b-contamination-from-raw-paprika-fruit-to-capsicum-oleoresin-during-the-extraction-process
#5
Naiying Wu, Wei Gao, Yunhe Lian, Jingjing Du, Xiaowei Tie
Occurrence of Rhodamine B (RhB) contamination in paprika caused by agricultural materials during the vegetation process has been reported. It may transfer during the process of active compounds extraction, and eventually exist in final products. Herein, the re-distribution of RhB during the extraction process was assessed in terms of RhB contents, as well as mass, color value and capsaicinoids yield of each process. Results revealed that natural RhB contamination at 0.55-1.11µg/kg originated from raw paprika fruit then transferred with the extraction proceeded...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763001/functional-and-promoter-analysis-of-chiiv3-a-chitinase-of-pepper-plant-in-response-to-phytophthora-capsici-infection
#6
Zhiqin Liu, Lanping Shi, Sheng Yang, Youquan Lin, Yahong Weng, Xia Li, Ansar Hussain, Ali Noman, Shuilin He
Despite the involvement of many members of the chitinase family in plant immunity, the precise functions of the majority of the members remain poorly understood. Herein, the gene ChiIV3 in Capsicum annuum encoding a chitinase protein containing a chitin binding domain and targeting to the plasma membrane was found to be induced by Phytophthora capsici inoculation (PCI) and applied chitin treatment. Besides its direct inhibitory effect on growth of Phytophthora capsici (P. capsici), ChiIV3 was also found by virus-induced gene silencing (VIGS) and transient overexpression (TOE) in pepper plants to act as a positive regulator of plant cell death and in triggering defense signaling and upregulation of PR (pathogenesis related) genes against PCI...
August 1, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28759173/direct-online-extraction-and-determination-by-supercritical-fluid-extraction-with-chromatography-and-mass-spectrometry-of-targeted-carotenoids-from-red-habanero-peppers-capsicum-chinense-jacq
#7
Mariosimone Zoccali, Daniele Giuffrida, Paola Dugo, Luigi Mondello
Recently, supercritical fluid chromatography coupled to mass spectrometry has gained attention as a fast and useful technology applied to the carotenoids analysis. However no reports are available in the literature on the direct on-line extraction and determination, by supercritical fluid extraction with chromatography and mass spectrometry. The aim of this research was focused on the development of an online method coupling supercritical fluid extraction and supercritical fluid chromatography for a detailed targeted native carotenoids characterization in red habanero peppers...
July 31, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28751388/rhizospheric-microbiome-profiling-of-capsicum-annuum-l-cultivated-in-amended-soils-by-16s-and-internal-transcribed-spacer-2-rrna-amplicon-metagenome-sequencing
#8
Ali Asaff-Torres, Mariana Armendáriz-Ruiz, Manuel Kirchmayr, Raúl Rodríguez-Heredia, Marcos Orozco, Juan Carlos Mateos-Díaz, Luis Figueroa-Yáñez, Itzamná Baqueiro-Peña, Jorge Verdín
Rhizospheric microbiomes of Capsicum annuum L. cultivated either conventionally or amended with a synthetic microbial consortium or a root exudate inductor, were characterized by 16S/internal transcribed spacer 2 (ITS2) rRNA amplicon metagenome sequencing. The most abundant taxa found, although differently represented in each treatment, were Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, and Bacilli, as well as Chytridiomycetes and Mortierellomycotina.
July 27, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28747860/lipolytic-efficacy-of-alginate-double-layer-nanoemulsion-containing-oleoresin-capsicum-in-differentiated-3t3-l1-adipocytes
#9
Mak-Soon Lee, Sunyoon Jung, Yoonjin Shin, Seohyun Lee, Chong-Tai Kim, In-Hwan Kim, Yangha Kim
Background: Oleoresin capsicum (OC) is an organic extract from fruits of the genus Capsicum, and has been reported to have an anti-obesity effect. Objective: This study comparatively investigated lipolytic effects of single-layer nanoemulsion (SN) and alginate double-layer nanoemulsion (AN) containing OC in 3T3-L1 adipocytes. Methods: SN and AN were compared by analyzing the intracellular lipid accumulation, triglyceride (TG) content, release of free fatty acids (FFAs) and glycerol, and mRNA expression of genes related to adipogenesis and lipolysis were analyzed in fully differentiated 3T3-L1 adipocytes...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28746617/resistance-of-xanthomonas-euvesicatoria-strains-from-brazilian-pepper-to-copper-and-zinc-sulfates
#10
Maysa S Areas, Ricardo M Gonçalves, José M Soman, Ronaldo C Souza, Ricardo Gioria, Tadeu A F DA Silva, Antonio C Maringoni
Bacterial spot, caused by Xanthomonas spp., is one of the major bacterial diseases in pepper (Capsicum annuum L.). The infection results in reduced crop yield, particularly during periods of high rainfall and temperature, due to the low efficiency of chemical control with copper bactericides. This study evaluated the copper and zinc sulfate sensitivity of 59 pathogenic strains of Xanthomonas euvesicatoria isolated from pepper plants produced in various regions throughout Brazil. Both the respective sulfates and a mixture thereof was evaluated at 50, 100, 200 and 400 μg...
July 24, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28744701/aging-and-visual-3-d-shape-recognition-from-motion
#11
J Farley Norman, Olivia C Adkins, Catherine J Dowell, Stevie C Hoyng, Lindsey M Shain, Lauren E Pedersen, Jonathan D Kinnard, Alexia J Higginbotham, Ashley N Gilliam
Two experiments were conducted to evaluate the ability of younger and older adults to recognize 3-D object shape from patterns of optical motion. In Experiment 1, participants were required to identify dotted surfaces that rotated in depth (i.e., surface structure portrayed using the kinetic depth effect). The task difficulty was manipulated by limiting the surface point lifetimes within the stimulus apparent motion sequences. In Experiment 2, the participants identified solid, naturally shaped objects (replicas of bell peppers, Capsicum annuum) that were defined by occlusion boundary contours, patterns of specular highlights, or combined optical patterns containing both boundary contours and specular highlights...
July 25, 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/28742940/functional-analysis-of-the-pepper-protein-phosphatase-caaipp1-and-its-interacting-partner-caairf1-modulation-of-aba-signaling-and-the-drought-stress-response
#12
Woonhee Baek, Chae Woo Lim, Sung Chul Lee
Plant adaptive responses to abiotic stress are coordinated by restriction of plant growth and development. The plant hormone abscisic acid (ABA) is the key regulator of the response to abiotic stress, and its sensitivity determines abiotic stress tolerance levels. We previously showed that the E3 ubiquitin ligase CaAIRF1 functions as a positive regulator of ABA and drought stress via modulation of transcription and stability of the type 2C protein phosphatase CaADIP1. Here, we report the identification and functional analysis of a novel type 2C phosphatase, CaAIPP1 (Capsicum annuum CaAIRF1 Interacting Protein Phosphatase 1)...
July 25, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28736938/genetic-variation-in-pythium-myriotylum-based-on-snp-typing-and-development-of-a-pcr-rflp-detection-of-isolates-recovered-from-pythium-soft-rot-ginger
#13
Duy P Le, Mike K Smith, Elizabeth A B Aitken
Pythium myriotylum is responsible for severe losses in both capsicum and ginger crops in Australia under different regimes. Intraspecific genomic variation within the pathogen might explain the differences in aggressiveness and pathogenicity on diverse hosts. In this study, whole genome data of four P. myriotylum isolates recovered from three hosts and one P. zingiberis isolate were derived and analysed for sequence diversity based on single nucleotide polymorphisms (SNPs). A higher number of true and unique SNPs occurred in P...
July 23, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28721659/antimicrobial-activity-of-selected-polyphenols-and-capsaicinoids-identified-in-pepper-capsicum-annuum-l-and-their-possible-mode-of-interaction
#14
Meriem Mokhtar, Giovanna Ginestra, Fatma Youcefi, Angela Filocamo, Carlo Bisignano, Ali Riazi
Antimicrobial activity of pepper polyphenols and capsaicinoids (Coumarin, caffeic acid, narangin, kaempferol, rutin, quercetin, capsaicin, and dihydrocapsaicin) against 13 pathogen bacteria and three beneficial strains was studied using the disk diffusion and microdilution methods. In general, phenolic compounds had the most important activity with the highest inhibition zones obtained with caffeic acid (3.5-20.5 mm), quercetin (4.75-3.5 mm), and kaempferol (7-14 mm). In the determination of the minimal inhibitory concentrations, the effects of both quercetin and kaempferol were more important than caffeic acid...
July 18, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28720957/bioactive-compounds-and-antioxidant-activity-in-scalded-jalape%C3%A3-o-pepper-industrial-byproduct-capsicum-annuum
#15
Claudia Jaqueline Sandoval-Castro, Maribel Valdez-Morales, B Dave Oomah, Roberto Gutiérrez-Dorado, Sergio Medina-Godoy, L Gabriela Espinosa-Alonso
Bioactive compounds and antioxidant activity were evaluated from industrial Jalapeño pepper byproducts and simulated non processed byproducts from two Mexican states (Chihuahua and Sinaloa) to determine their value added potential as commercial food ingredients. Aqueous 80% ethanol produced about 13% of dry extract of polar compounds. Total phenolic content increased and capsaicin and dihydrocapsaicin decreased on scalding samples (80 °C, 2 min) without affecting ascorbic acid. The major phenolic compounds, rutin, epicatechin and catechin comprised 90% of the total compounds detected by HPLC of each Jalapeño pepper byproducts...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28714761/gamma-irradiation-of-medicinally-important-plants-and-the-enhancement-of-secondary-metabolite-production
#16
P Vivek Vardhan, Lata I Shukla
PURPOSE: The profitable production of some important plant-based secondary metabolites (ginsenosides, saponins, camptothecin, shikonins etc.) in vitro by gamma irradiation is a current area of interest. We reviewed different types of secondary metabolites, their mode of synthesis and effect of γ-radiation on their yield for different plants, organs and in vitro cultures (callus, suspension, hairy root). Special effort has been made to review the biochemical mechanisms underlying the increase in secondary metabolites...
July 17, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28706523/identification-of-optimal-reference-genes-for-normalization-of-qpcr-analysis-during-pepper-fruit-development
#17
Yuan Cheng, Xin Pang, Hongjian Wan, Golam J Ahammed, Jiahong Yu, Zhuping Yao, Meiying Ruan, Qingjing Ye, Zhimiao Li, Rongqing Wang, Yuejian Yang, Guozhi Zhou
Due to its high sensitivity and reproducibility, quantitative real-time PCR (qPCR) is practiced as a useful research tool for targeted gene expression analysis. For qPCR operations, the normalization with suitable reference genes (RGs) is a crucial step that eventually determines the reliability of the obtained results. Although pepper is considered an ideal model plant for the study of non-climacteric fruit development, at present no specific RG have been developed or validated for the qPCR analyses of pepper fruit...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28702041/characterization-of-bip-genes-from-pepper-capsicum-annuum-l-and-the-role-of-cabip1-in-response-to-endoplasmic-reticulum-and-multiple-abiotic-stresses
#18
Hu Wang, Huanhuan Niu, Yufei Zhai, Minghui Lu
Adverse environmental conditions have a detrimental impact on crop growth and development, and cause protein denaturation or misfolding. The binding protein (BiP) plays an important protective role by alleviating endoplasmic reticulum (ER) stress induced by misfolded proteins. In this study, we characterized three BiP genes (CaBiP1, CaBiP2, and CaBiP3) in pepper, an economically important vegetable and spice species. The role of CaBiP1 in plant tolerance to ER stress and adverse environmental conditions (including heat, salinity, osmotic and drought stress) were investigated...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28702034/cardr1-an-rna-dependent-rna-polymerase-plays-a-positive-role-in-pepper-resistance-against-tmv
#19
Lei Qin, Ning Mo, Yang Zhang, Tayeb Muhammad, Guiye Zhao, Yan Zhang, Yan Liang
RNA silencing functions as a major natural antiviral defense mechanism in plants. RNA-dependent RNA polymerases (RDRs) that catalyze the synthesis of double-stranded RNAs, are considered as a fundamental element in RNA silencing pathways. In Arabidopsis thaliana, RDR1, 2 and 6 play important roles in anti-viral RNA silencing. Expression of RDR1 can be elevated following plant treatment with defense hormones and virus infection. RDR1 has been studied in several crop species, but not in pepper (Capsicum annuum L...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28692124/genetic-variability-of-a-brazilian-capsicum-frutescens-germplasm-collection-using-morphological-characteristics-and-ssr-markers
#20
S I C Carvalho, L B Bianchetti, C F Ragassi, C S C Ribeiro, F J B Reifschneider, G S C Buso, F G Faleiro
Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - SSR) molecular markers. Morphological characterization was carried out using 57 descriptors, and molecular characterization was based on 239 alleles from 24 microsatellite loci...
July 6, 2017: Genetics and Molecular Research: GMR
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