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https://www.readbyqxmd.com/read/28631818/inactivation-of-bacillus-cereus-spores-on-red-chili-peppers-using-a-combined-treatment-of-aqueous-chlorine-dioxide-and-hot-air-drying
#1
Songyi Kim, Huyong Lee, Jee-Hoon Ryu, Hoikyung Kim
The effect of a combined treatment using aqueous chlorine dioxide (ClO2 ) and hot-air drying to inactivate Bacillus cereus spores on red chili peppers was evaluated. Ten washed and dried pepper samples, each comprising half of a single pepper (Capsicum annuum L.), were inoculated with B. cereus spore suspension. The inoculated samples were washed with sodium hypochlorite (NaOCl; 50, 100, or 200 μg/mL) or ClO2 (50, 100, or 200 μg/mL) solution for 1 min and then air-dried (25 ± 1 °C, 47 ± 1% relative humidity), which was followed by drying with hot air at 55 °C for up to 48 h...
June 20, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28631815/expansion-of-sesquiterpene-biosynthetic-gene-clusters-in-pepper-confers-nonhost-resistance-to-the-irish-potato-famine-pathogen
#2
Hyun-Ah Lee, Sejun Kim, Seungill Kim, Doil Choi
Chemical barriers contribute to nonhost resistance, which is defined as the resistance of an entire plant species to nonadapted pathogen species. However, the molecular basis of metabolic defense in nonhost resistance remains elusive. Here, we report genetic evidence for the essential role of phytoalexin capsidiol in nonhost resistance of pepper (Capsicum spp.) to potato late blight Phytophthora infestans using transcriptome and genome analyses. Two different genes for capsidiol biosynthesis, 5-epi-aristolochene synthase (EAS) and 5-epi-aristolochene-1,3-dihydroxylase (EAH), belong to multigene families...
June 20, 2017: New Phytologist
https://www.readbyqxmd.com/read/28624494/antibacterial-activity-and-phospholipid-recognition-of-the-recombinant-defensin-j1-1-from-capsicum-genus
#3
Francisco Guillén-Chable, Iván Arenas-Sosa, Ignacio Islas-Flores, Gerardo Corzo, Cynthia Martinez-Liu, Georgina Estrada
The gene of the four disulfide-bridged defensin J1-1 from Capsicum was cloned into the expression vector pQE30 containing a 6His-tag as fusion protein. This construct was transfected into Origami strain of Escherichia coli and expressed after induction with isopropyl thiogalactoside (IPTG). The level of expression was 4 mg/L of culture medium, and the His-tagged recombinant defensin (HisXarJ1-1) was expressed exclusively into inclusion bodies. After solubilization, HisXarJ1-1 was purified by affinity and hydrophobic interaction chromatography...
June 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28612389/biochar-potential-in-intensive-cultivation-of-capsicum-anuum-l-sweet-pepper-crop-yield-and-plant-protection
#4
Abhay Kumar, Yigal Elad, Ludmila Tsechansky, Vikas Abrol, Beni Lew, Rivka Offenbach, Ellen R Graber
BACKGROUND: The influence of various biochars on crop yield and disease resistance of Capsicum anuum L. (sweet pepper) under modern, high-input, intensive net house cultivation was tested over the course of 2011 to 2014 in the Arava desert region of Israel. A pot experiment with Lactuca sativa L. (lettuce) grown in the absence of fertilizer employed the 3-year old field trial soils to determine if biochar treatments contributed to soil intrinsic fertility. RESULTS: Biochar amendments resulted in a significant increase in number and weight of pepper fruits over three years...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28588235/parasitic-nematode-meloidogyne-incognita-interactions-with-different-capsicum-annum-cultivars-reveal-the-chemical-constituents-modulating-root-herbivory
#5
Ruth Kihika, Lucy K Murungi, Danny Coyne, Margaret Ng'ang'a, Ahmed Hassanali, Peter E A Teal, Baldwyn Torto
Plant volatile signatures are often used as cues by herbivores to locate their preferred hosts. Here, we report on the volatile organic compounds used by the subterranean root-knot nematode (RKN) Meloidogyne incognita for host location. We compared responses of infective second stage juveniles (J2s) to root volatiles of three cultivars and one accession of the solanaceous plant, Capsicum annum against moist sand in dual choice assays. J2s were more attracted to the three cultivars than to the accession, relative to controls...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28584274/overexpression-of-pea-dna-helicase-45-pdh45-imparts-tolerance-to-multiple-abiotic-stresses-in-chili-capsicum-annuum-l
#6
Tagginahalli N Shivakumara, Rohini Sreevathsa, Prasanta K Dash, M S Sheshshayee, Pradeep K Papolu, Uma Rao, Narendra Tuteja, M UdayaKumar
Imparting tolerance to abiotic stresses is of global importance as they inflict significant yield losses in field as well as in vegetable crops. Transcriptional activators, including helicases are identified to play a pivotal role in stress mitigation. Helicases, also known as molecular motors, are involved in myriad cellular processes that impart intrinsic tolerance to abiotic stresses in plants. Our study demonstrates the potential of a Pea DNA Helicase 45 (PDH45), in combating multiple abiotic stresses in chili...
June 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28578966/new-findings-on-green-sweet-pepper-capsicum-annum-pectins-rhamnogalacturonan-and-type-i-and-ii-arabinogalactans
#7
Georgia Erdmann do Nascimento, Marcello Iacomini, Lucimara M C Cordeiro
Polysaccharides were extracted from sweet pepper (Capsicum annum) with hot water and named ANW (9% yield). Starch was precipitated by freeze-thaw treatment, while pectic polysaccharides (8% yield) remained soluble and consisted of GalA (67.0%), Rha (1.6%), Ara (6.4%), Xyl (0.3%), Gal (6.7%) and Glc (4.4%). A highly methoxylated homogalacturonan (HG, degree of methylesterification of 85% and degree of acetylation of 5%), and type I and type II arabinogalactans (AG-I and AG-II) were observed in NMR analyses. These were fractionated with Fehling's solution to give HG (5...
September 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28573647/brazilian-capsicum-peppers-capsaicinoids-content-and-antioxidant-activity
#8
Stanislau Bogusz, Silvia H Libardi, Fernanda F G Dias, Janclei P Coutinho, Vivian C Bochi, Daniele Rodrigues, Arlete M T Melo, Helena T Godoy
BACKGROUND: Capsicum peppers are known as a source of capsaicinoids, phenolic compounds, and antioxidants. Brazilian Capsicum peppers are important spices use in foods worldwide. However, little information is available on the chemical composition and antioxidant activity of these peppers. RESULTS: Capsaicin, dihydrocapsaicin, total phenolic compounds, and antioxidant activity were investigated in the extracts of three Brazilian peppers: Capsicum frutescens, C. chinense and C...
June 2, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28572963/antibacterial-activity-of-jalape%C3%A3-o-pepper-capsicum-annuum-var-annuum-extract-fractions-against-select-foodborne-pathogens
#9
Karleigh Bacon, Renee Boyer, Cynthia Denbow, Sean O'Keefe, Andrew Neilson, Robert Williams
Capsicum annuum fruits have been investigated for antimicrobial activity in a number of studies. Capsaicin or other cinnamic acid pathway intermediates are often suggested to be the antimicrobial component, however there are conflicting results. No research has specifically fractionated jalapeño pepper (Capsicum annuum var. annuum) extract to isolate and identify compound(s) responsible for inhibition. In this study, fractions were collected from jalapeño pepper extracts using reverse-phase HPLC and tested for antibacterial activity using the disk diffusion method...
May 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28572934/evaluation-of-different-solvents-to-extract-antibacterial-compounds-from-jalape%C3%A3-o-peppers
#10
Karleigh Bacon, Renee Boyer, Cynthia Denbow, Sean O'Keefe, Andrew Neilson, Robert Williams
Chili peppers (Capsicum spp.) may possess antibacterial properties and have potential to be used in foods as antimicrobial. The complete chili pepper extract should be evaluated to determine which compounds are responsible for the antimicrobial activity. Extraction of compounds from the pepper is completed using a solvent. The type of solvent used for extraction influences which compounds are isolated, therefore the best extraction method needs to be determined. The purpose of this study was to identify which solvent is most successful at extracting unknown antibacterial compounds from jalapeño peppers...
May 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28569248/effect-of-foliar-application-of-salicylic-acid-hydrogen-peroxide-and-a-xyloglucan-oligosaccharide-on-capsiate-content-and-gene-expression-associatedwith-capsinoids-synthesis-in-capsicum-annuum-l
#11
A Y Zunun-Perez, T Guevara-Figueroa, S N Jimenez-Garcia, A A Feregrino-Perez, F Gautier, R G Guevara-Gonzalez
Capsinoids are non-pungent analogues of capsaicinoids in pepper (Capsicum spp). The absence of pungency, in addition to their biological activities similar to that of capsaicinoids such as anti-inflammatory, antimicrobial, and antioxidant properties, makes capsinoids an excellent option for increasing use in human and animal nutrition, as well as health and pharmaceutical industries. There are only few sources of pepper producing capsinoids, and one of them (accession 509-45-1), Capsicum annuum L., is a potential source for increasing capsinoids content using strategies as controlled elicitation during plant production in the greenhouse...
June 2017: Journal of Biosciences
https://www.readbyqxmd.com/read/28567626/isolation-characterization-and-genetic-diversity-of-nbs-lrr-class-disease-resistant-gene-analogs-in-multiple-virus-resistant-line-of-chilli-capsicum-annuum-l
#12
P Naresh, M Krishna Reddy, Anand C Reddy, B Lavanya, D C Lakshmana Reddy, K Madhavi Reddy
Viruses are serious threat to chilli crop production worldwide. Resistance screening against several viruses resulted in identifying a multiple virus resistant genotype 'IHR 2451'. Degenerate primers based on the conserved regions between P-Loop and GLPL of Resistance genes (R-genes) were used to amplify nucleotide binding sites (NBS)-encoding regions from genotype 'IHR 2451'. Alignment of deduced amino acid sequences and phylogenetic analyses of isolated sequences distinguished into two groups representing toll interleukin-1 receptor (TIR) and non-TIR, and different families within the group confirming the hypotheses that dicots have both the types of NBS-LRR genes...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28536285/gut-microbiota-mediates-the-protective-effects-of-dietary-capsaicin-against-chronic-low-grade-inflammation-and-associated-obesity-induced-by-high-fat-diet
#13
Chao Kang, Bin Wang, Kanakaraju Kaliannan, Xiaolan Wang, Hedong Lang, Suocheng Hui, Li Huang, Yong Zhang, Ming Zhou, Mengting Chen, Mantian Mi
Metabolic endotoxemia originating from dysbiotic gut microbiota has been identified as a primary mediator for triggering the chronic low-grade inflammation (CLGI) responsible for the development of obesity. Capsaicin (CAP) is the major pungent bioactivator in chili peppers and has potent anti-obesity functions, yet the mechanisms linking this effect to gut microbiota remain obscure. Here we show that mice fed a high-fat diet (HFD) supplemented with CAP exhibit lower levels of metabolic endotoxemia and CLGI associated with lower body weight gain...
May 23, 2017: MBio
https://www.readbyqxmd.com/read/28527512/protective-effect-of-capsaicin-against-methyl-methanesulphonate-induced-toxicity-in-the-third-instar-larvae-of-transgenic-drosophila-melanogaster-hsp70-lacz-bg-9
#14
Saba Khanam, Ambreen Fatima, Rahul Smita Jyoti, Fahad Ali, Falaq Naz, Barkha Shakya, Yasir Hasan Siddique
Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is the main component in hot peppers, including red chili peppers, jalapenos, and habanero, belonging to the genus Capsicum. Capsaicin is a potent antioxidant that interferes with free radical activities. In the present study, the possible protective effect of capsaicin was studied against methyl methanesulphonate (MMS) induced toxicity in third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg(9). The third instar was allowed to feed on the diet having different doses of capsaicin and MMS separately and in combination...
April 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28508261/functional-roles-of-the-pepper-leucine-rich-repeat-protein-and-its-interactions-with-pathogenesis-related-and-hypersensitive-induced-proteins-in-plant-cell-death-and-immunity
#15
REVIEW
Jeum Kyu Hong, In Sun Hwang, Byung Kook Hwang
Pepper leucine-rich repeat protein (CaLRR1) interacts with defense response proteins to regulate plant cell death and immunity. This review highlights the current understanding of the molecular functions of CaLRR1 and its interactor proteins. Plant cell death and immune responses to microbial pathogens are controlled by complex and tightly regulated molecular signaling networks. Xanthomonas campestris pv. vesicatoria (Xcv)-inducible pepper (Capsicum annuum) leucine-rich repeat protein 1 (CaLRR1) serves as a molecular marker for plant cell death and immunity signaling...
May 15, 2017: Planta
https://www.readbyqxmd.com/read/28507559/the-dnaj-gene-family-in-pepper-capsicum-annuum-l-comprehensive-identification-characterization-and-expression-profiles
#16
FangFei Fan, Xian Yang, Yuan Cheng, Yunyan Kang, Xirong Chai
The DnaJ proteins which function as molecular chaperone played critical roles in plant growth and development and response to heat stress (HS) and also called heat shock protein 40 based on molecular weight. However, little was reported on this gene family in pepper. Recently, the release of the whole pepper genome provided an opportunity for identifying putative DnaJ homologous. In this study, a total of 76 putative pepper DnaJ genes (CaDnaJ01 to CaDnaJ76) were identified using bioinformatics methods and classified into five groups by the presence of the complete three domains (J-domain, zinc finger domain, and C-terminal domain)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28506257/an-overview-of-systematic-reviews-of-complementary-and-alternative-therapies-for-fibromyalgia-using-both-amstar-and-robis-as-quality-assessment-tools
#17
Rachel Perry, Verity Leach, Philippa Davies, Chris Penfold, Andy Ness, Rachel Churchill
BACKGROUND: Fibromyalgia (FM) is a chronic, debilitating pain disorder. Dissatisfaction with conventional medicine can lead people with FM to turn to complementary and alternative medicine (CAM). Two previous overviews of systematic reviews of CAM for FM have been published, but they did not assessed for risk of bias in the review process. METHODS: Five databases Medline, Embase, AMED (via OVID), Web of Science and Central were searched from their inception to December 2015...
May 15, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/28503186/the-pepper-ring-finger-e3-ligase-cadir1-regulates-the-drought-stress-response-via-aba-mediated-signaling
#18
Hyunhee Joo, Chae Woo Lim, Sang-Wook Han, Sung Chul Lee
Drought stress from soil or air limits plant growth and development, leading to a reduction in crop productivity. Several E3 ligases positively or negatively regulate the drought stress response. In the present study, we show that the pepper (Capsicum annuum) Drought Induced RING type E3 ligase 1, CaDIR1, regulates the drought stress response via abscisic acid (ABA)-mediated signaling. CaDIR1 contains a C3HC4-type RING finger domain in the N-terminal region; this domain functions during protein degradation via attachment of ubiquitins to the substrate target proteins...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28497560/untargeted-metabolomic-analysis-of-capsicum-spp-by-gc-ms
#19
Bianca Camargo Aranha, Jessica Fernanda Hoffmann, Rosa Lia Barbieri, Cesar Valmor Rombaldi, Fábio Clasen Chaves
INTRODUCTION: In order to conserve the biodiversity of Capsicum species and find genotypes with potential to be utilised commercially, Embrapa Clima Temperado maintains an active germplasm collection (AGC) that requires characterisation, enabling genotype selection and support for breeding programmes. OBJECTIVE: The objective of this study was to characterise pepper accessions from the Embrapa Clima Temperado AGC and differentiate species based on their metabolic profile using an untargeted metabolomics approach...
May 11, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28483879/an-r2r3-myb-transcription-factor-in-capsaicinoid-biosynthesis
#20
Magda Lisette Arce-Rodríguez, Neftalí Ochoa-Alejo
Capsaicinoids are responsible for the hot taste of chili peppers. They are restricted to the genus Capsicum and are synthesized by the acylation of the aromatic compound vanillylamine (derived from the phenylpropanoid pathway) with a branched-chain fatty acid by the catalysis of the putative enzyme capsaicinoid synthase. R2R3-MYB transcription factors have been reported in different species of plants as regulators of structural genes of the phenylpropanoid pathway; therefore, we hypothesized that MYB genes might be involved in the regulation of the biosynthesis of pungent compounds...
May 8, 2017: Plant Physiology
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