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flow cytometry in sarcoidosis

Marcial Sebode, Sören Weidemann, Malte Wehmeyer, Ansgar W Lohse, Christoph Schramm
We present a case of hepatosplenic necrotizing sarcoid granulomatosis, a variant form of "classical" sarcoidosis, that became clinically apparent in the form of multiple hepatic and splenic masses mimicking malignancy. Flow cytometry of intrahepatic T cells isolated from liver biopsy led to the targeted treatment with anti-tumor necrosis factor-alpha, which was highly effective in inducing remission. (Hepatology 2017;65:1410-1412).
April 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Simon D Fraser, Laura R Sadofsky, Paul M Kaye, Simon P Hart
In sarcoidosis, the proinflammatory cytokines interferon gamma, tumour necrosis factor and interleukin-6 are released by monocyte-derived macrophages and lymphocytes in the lungs and other affected tissues. Regulatory receptors expressed on monocytes and macrophages act to suppress cytokine production, and reduced expression of regulatory receptors may thus promote tissue inflammation. The aim of this study was to characterise the role of regulatory receptors on blood monocytes in patients with sarcoidosis...
December 8, 2016: Scientific Reports
Zdenka Navratilova, Eva Novosadova, Michael Hagemann-Jensen, Susanna Kullberg, Vitezslav Kolek, Johan Grunewald, Martin Petrek
BACKGROUND: Sarcoidosis is characterised by up-regulation of cytokines and chemokine ligands/receptors and proteolytic enzymes. This pro-inflammatory profile is regulated post-transcriptionally by RNA-binding proteins (RBPs). We investigated in vivo expression of six RBPs (AUF1, HuR, NCL, TIA, TIAR, PCBP2) and two inhibitors of proteolytic enzymes (RECK, PTEN) in pulmonary sarcoidosis and compared it to the expression in four control groups of healthy individuals and patients with other respiratory diseases: chronic obstructive pulmonary disease (COPD), asthma and idiopathic interstitial pneumonias (IIPs)...
2016: PloS One
Maria-Jose Martinez-Bravo, Casper J E Wahlund, Khaleda Rahman Qazi, Robert Moulder, Ana Lukic, Olof Rådmark, Riitta Lahesmaa, Johan Grunewald, Anders Eklund, Susanne Gabrielsson
BACKGROUND: Sarcoidosis is an inflammatory granulomatous disorder characterized by accumulation of TH1-type CD4(+) T cells and immune effector cells within affected organs, most frequently the lungs. Exosomes are extracellular vesicles conveying intercellular communication with possible diagnostic and therapeutic applications. OBJECTIVES: We aimed to provide an understanding of the proinflammatory role of bronchoalveolar lavage fluid (BALF) exosomes in patients with sarcoidosis and to find candidates for disease biomarkers...
April 2017: Journal of Allergy and Clinical Immunology
Sabine Ringkowski, Joshua Loke, Shuying Huang, Hasib Ahmadzai, Felix J F Herth, Paul S Thomas, Cristan Herbert
RATIONALE: Granulomas in sarcoidosis have recently been described as containing Interleukin (IL)-27, one of the members of the IL-12 family of cytokines, which also includes IL-35. Levels of these cytokines and the IL-27 receptor subunits were hypothesised to differ between patients with sarcoidosis compared to healthy controls in peripheral blood. METHODS: Using a cross-sectional study design, plasma and peripheral blood mononuclear cells (PBMC) were collected from patients and control subjects...
August 2016: Respiratory Medicine
Sory J Ruiz, Yaxia Zhang, Sanjay Mukhopadhyay
BACKGROUND: Despite mixed results in the literature, some clinicians continue to consider an elevated CD4/CD8 ratio in bronchoalveolar lavage (BAL) fluid to be supportive of a diagnosis of sarcoidosis. However, the CD4/CD8 ratio in mediastinal lymph nodes involved by sarcoidosis has not been extensively studied. The primary aim of this study was to evaluate the utility of the CD4/CD8 ratio in mediastinal lymph node aspirates obtained by endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) for diagnosing sarcoidosis...
October 2016: Journal of Bronchology & Interventional Pulmonology
Mona Mlika, Nourane Kria, Emna Braham, Chokri Chebbi, Faouzi El Mezni
Multidisciplinary concertation is mandatory in order to assess interstitial pneumonias. The study of the bronchoalveolar lavage helps evoking a diagnosis according to the lavage profile. In lymphocytic alveolitis, immunocytochemistry, or in flux cytometry are necessary in order to identify the different clusters of lymphocytes implicated. Our objective was to evaluate the profile of 31 lymphocytic alveolitis using 2 different techniques which are the immunocytochemistry and the in flow cytometry in order to evaluate the efficacy of each technique and to compare the different results to the final diagnoses...
2017: Journal of Immunoassay & Immunochemistry
Takahiro Nakajima, Kazuhiro Yasufuku, Taiki Fujiwara, Ichiro Yoshino
Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a minimally invasive modality for sampling of mediastinal and hilar lymph nodes as well as pulmonary lesions adjacent to the airway. Guidelines for staging of lung cancer suggest that EBUS-TBNA should be considered the best first test of nodal staging for radiologically abnormal lymph nodes that are accessible by this approach. The application of EBUS-TBNA in pulmonary medicine and thoracic oncology is expanding with its role in the diagnosis of sarcoidosis, lymphoma, and tuberculosis...
July 2016: Respiratory Investigation
Jasmin Schnerch, Antje Prasse, Dimitrios Vlachakis, Kathrin L Schuchardt, Dmitri V Pechkovsky, Torsten Goldmann, Karoline I Gaede, Joachim Müller-Quernheim, Gernot Zissel
Sarcoidosis is a granulomatous disease characterized by a T-helper type 1 (Th1) cell-dominated alveolitis. As a role of bacteria in the pathogenesis of sarcoidosis has been discussed, Toll-like receptors (TLRs) may be involved in the initiation of a first immune reaction. We analyzed expression and functional relevance of several TLRs in bronchoalveolar lavage (BAL) cells from patients with pulmonary sarcoidosis. In parallel, we determined the release of C-X-C motif chemokine 9 (CXCL9), CXCL10, and CXCL11 by BAL cells from patients with pulmonary sarcoidosis...
November 2016: American Journal of Respiratory Cell and Molecular Biology
Ylva Kaiser, Rico Lepzien, Susanna Kullberg, Anders Eklund, Anna Smed-Sörensen, Johan Grunewald
Disease phenotypes of pulmonary sarcoidosis are distinguished by clinical rather than immunological criteria. We aimed to characterise patterns of CD4(+) T-cell lineage plasticity underlying the differences in clinical presentation and disease course between the acute form, Löfgren's syndrome, and the heterogeneous, potentially progressive "non-Löfgren" form.33 pulmonary sarcoidosis patients and nine controls underwent bronchoscopy with bronchoalveolar lavage. CD4(+) T-cell transcription factor, chemokine receptor and T-cell receptor expression, proliferation and cytokine production were assessed in the lavage fluid and peripheral blood using flow cytometry and multicolour FluoroSpot...
August 2016: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Lisa Bretagne, Ibrahima-Dina Diatta, Mohamed Faouzi, Antoine Nobile, Massimo Bongiovanni, Laurent P Nicod, Romain Lazor
BACKGROUND: The CD103 integrin is present on CD4+ lymphocytes of the bronchial mucosa, but not on peripheral blood CD4+ lymphocytes. It has been hypothesized that CD4+ lymphocytes in pulmonary sarcoidosis originate from redistribution from the peripheral blood to the lung, and therefore do not bear the CD103 integrin. Some data suggest that a low CD103+ percentage among bronchoalveolar lavage fluid (BALF) CD4+ lymphocytes discriminates between sarcoidosis and other diagnoses. OBJECTIVE: To determine the diagnostic value of BALF CD103+ to identify sarcoidosis among other causes of alveolar lymphocytosis in a large retrospective case series...
2016: Respiration; International Review of Thoracic Diseases
Andrea Oleñik, Beatriz Gonzalo-Suárez, Marcelino Revenga, Ernesto Roldán, Mónica Vázquez, Francisco J Muñoz-Negrete
PURPOSE: To report the use of flow cytometry on aqueous fluid to diagnose sarcoidosis in a patient with recurrent granulomatous anterior uveitis. METHODS: Case report. RESULTS: Flow cytometry on aqueous fluid demonstrated a CD4/CD8 ratio >9.5, consistent with a diagnosis of sarcoidosis. CONCLUSIONS: Flow cytometry on aqueous fluid may offer an additional pathway for diagnosing sarcoid anterior uveitis.
August 2017: Ocular Immunology and Inflammation
Jianming Wu, Yunfang Li, Weihua Guan, Kevin Viken, David M Perlman, Maneesh Bhargava
Sarcoidosis is a multisystem granulomatous disorder that causes significant morbidity. Genetic factors contribute to sarcoidosis risks. In this study, we investigated whether copy number variations (CNVs) of FCGR3A (coding for FcγRIIIA) and FCGR3B (coding for FcγRIIIB) genes are associated with sarcoidosis susceptibility and whether the expressions of FcγRIIIA on NK cells and FcγRIIIB on neutrophils are altered in sarcoidosis patients. TaqMan real-time PCR assays were used to analyze the CNV of FCGR3A and FCGR3B genes...
July 2016: Human Genetics
Hakan Tanrıverdi, Fırat Uygur, Tacettin Örnek, Fatma Erboy, Bülent Altınsoy, Figen Atalay, Mustafa Çağatay Büyükuysal, İshak Özel Tekin, Mehmet Araslı, Müge Meltem Tor
BACKGROUND: Bronchoalveolar lavage is considered a helpful tool in the diagnosis of diffuse parenchimal lung diseases such as sarcoidosis. CD4/CD8 ratio is higly specific but not sensitive to distinguish sarcoidosis and other intestitial lung diseases. We aimed to compare the diagnostic value of CD4/CD8 ratio and other lmphocyte subpopulations such as CD3+16+56, CD103+, CD4+CD103+, CD8+CD103+ in bronchoalveolar lavage to distinguish sarcoidosis and other nonsarcoidosis interstitial lung diseases...
January 15, 2016: Sarcoidosis, Vasculitis, and Diffuse Lung Diseases: Official Journal of WASOG
Yongzhe Liu, Lan Qiu, Yanxun Wang, Halimulati Aimurola, Yuyue Zhao, Shan Li, Zuojun Xu
OBJECTIVES: Pulmonary sarcoidosis is an immune-mediated disease, and some patients can be effectively treated with corticosteroids. However, nearly half of all sarcoidosis patients relapse after corticosteroid withdrawal. Different subsets of CD4+ helper T cells participate in the immunopathogenesis of sarcoidosis. Thus, the aims of our study were to investigate whether the circulating subsets of CD4+ helper T cells were associated with sarcoidosis relapse and with its remission after retreatment...
2016: PloS One
Johan Grunewald, Ylva Kaiser, Mahyar Ostadkarampour, Natalia V Rivera, Francesco Vezzi, Britta Lötstedt, Remi-André Olsen, Lina Sylwan, Sverker Lundin, Max Käller, Tatiana Sandalova, Kerstin M Ahlgren, Jan Wahlström, Adnane Achour, Marcus Ronninger, Anders Eklund
In pulmonary sarcoidosis, CD4(+) T-cells expressing T-cell receptor Vα2.3 accumulate in the lungs of HLA-DRB1*03(+) patients. To investigate T-cell receptor-HLA-DRB1*03 interactions underlying recognition of hitherto unknown antigens, we performed detailed analyses of T-cell receptor expression on bronchoalveolar lavage fluid CD4(+) T-cells from sarcoidosis patients.Pulmonary sarcoidosis patients (n=43) underwent bronchoscopy with bronchoalveolar lavage. T-cell receptor α and β chains of CD4(+) T-cells were analysed by flow cytometry, DNA-sequenced, and three-dimensional molecular models of T-cell receptor-HLA-DRB1*03 complexes generated...
March 2016: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
P Sakthivel, J Grunewald, A Eklund, D Bruder, J Wahlström
Sarcoidosis is a granulomatous inflammatory disorder of unknown aetiology. The increased frequency of activated lung CD4(+) T cells with a T helper type 1 (Th1) cytokine profile in sarcoidosis patients is accompanied by a reduced proportion and/or impaired function of regulatory T cells (Tregs ). Here we evaluated the expression of the inducible co-stimulator (ICOS) on lung and blood CD4(+) T cell subsets in sarcoidosis patients with different prognosis, by flow cytometry. Samples from the deep airways were obtained by bronchoalveolar lavage (BAL)...
February 2016: Clinical and Experimental Immunology
M Facco, A Cabrelle, F Calabrese, A Teramo, F Cinetto, S Carraro, V Martini, F Calzetti, N Tamassia, M A Cassatella, G Semenzato, C Agostini
BACKGROUND: TNF-like ligand 1A (TL1A), a recently recognized member of the TNF superfamily, and its death domain receptor 3 (DR3), firstly identified for their relevant role in T lymphocyte homeostasis, are now well-known mediators of several immune-inflammatory diseases, ranging from rheumatoid arthritis to inflammatory bowel diseases to psoriasis, whereas no data are available on their involvement in sarcoidosis, a multisystemic granulomatous disease where a deregulated T helper (Th)1/Th17 response takes place...
2015: Clinical and Molecular Allergy: CMA
Tadeusz Płusa, Sylwia Chełstowska
UNLABELLED: Sarcoidosis is a disease of unclear etiology, immunopathogenesis complex and diverse clinical course. AIM: The aim of study was to evaluate the usefulness of CD34+ cells, CD4 and CD8 lymphocytes in peripheral blood for the diagnosis and better understanding of the sarcoidosis pathogenesis. MATERIALS AND METHODS: The study included 40 patients (16 women and 24 men) aged 29-71 years (mean 45 years) with newly diagnosed pulmonary sarcoidosis...
May 2015: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
Yue-song Weng, Hua-ying Wang, Ding-feng Lv, Zhong-ming Fu, Wan-jun Yu
OBJECTIVE: To investigate the Th17 cell and Treg cell levels in patients with sarcoidosis, and their relation to disease activation and glucocorticoids treatment. METHODS: Twenty-three sarcoidosis patients admitted in Yinzhou People's Hospital from January 2009 to December 2013 and 25 healthy subjects (controls) were included in this study. The blood samples and bronchoalveolar lavage fluid (BALF) samples were collected in all patients before and after glucocorticoids treatment...
March 2015: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
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