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https://www.readbyqxmd.com/read/28527697/antibiotics-induce-mitonuclear-protein-imbalance-but-fail-to-inhibit-respiration-and-nutrient-activation-in-pancreatic-%C3%AE-cells
#1
Jaime Santo-Domingo, Isabelle Chareyron, Charlotte Broenimann, Steve Lassueur, Andreas Wiederkehr
Chloramphenicol and several other antibiotics targeting bacterial ribosomes inhibit mitochondrial protein translation. Inhibition of mitochondrial protein synthesis leads to mitonuclear protein imbalance and reduced respiratory rates as confirmed here in HeLa and PC12 cells. Unexpectedly, respiration in INS-1E insulinoma cells and primary human islets was unaltered in the presence of chloramphenicol. Resting respiratory rates and glucose stimulated acceleration of respiration were also not lowered when a range of antibiotics including, thiamphenicol, streptomycin, gentamycin and doxycycline known to interfere with bacterial protein synthesis were tested...
May 17, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28527566/abnormalities-in-high-energy-phosphate-metabolism-in-first-episode-bipolar-disorder-measured-using-31-p-magnetic-resonance-spectroscopy
#2
Fei Du, Cagri Yuksel, Virginie-Anne Chouinard, Polly Huynh, Kyle Ryan, Bruce M Cohen, Dost Öngür
BACKGROUND: Brain energy metabolism is critical for supporting synaptic function and information processing. A growing body of evidence suggests abnormalities in brain bioenergetics in psychiatric disorders, including both bipolar disorder (BD) and schizophrenia. (31)P magnetic resonance spectroscopy provides a noninvasive window into these processes in vivo. Using this approach, we previously showed that patients with BD show normal adenosine triphosphate (ATP) and phosphocreatine levels at rest but cannot maintain normal ATP levels in the visual cortex during times of high energy demand (photic stimulation)...
April 7, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/28526627/dynamic-fatigue-measurement-of-human-erythrocytes-using-dielectrophoresis
#3
Yuhao Qiang, Jia Liu, E Du
Erythrocytes must undergo severe deformation to pass through narrow capillaries and submicronic splenic slits for several hundred thousand times in their normal lifespan. Studies of erythrocyte biomechanics have been mainly focused on cell deformability and rheology measured from a single application of stress and mostly under a static or quasi-static state using classical biomechanical techniques, such as optical tweezers and micropipette aspiration. Dynamic behavior of erythrocytes in response to cyclic stresses that contributes to the membrane failure in blood circulation is not fully understood...
May 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28520446/-diabetic-foot-syndrome-importance-of-calf-muscles-mr-spectroscopy-in-the-assessment-of-limb-ischemia-and-effect-of-revascularization
#4
Andrea Němcová, Michal Dubský, Alexandra Jirkovská, Petr Šedivý, Miloslav Drobný, Milan Hájek, Monika Dezortová, Robert Bém, Vladimíra Fejfarová, Anna Pyšná
AIM: The standard method for assessment of effect of revascularization in patients with diabetic foot (DF) and critical limb ischemia (CLI) is transcutaneous oxygen pressure (TcPO2). Phosphorus magnetic resonance spectroscopy (31P MRS) enables to evaluate oxidative muscle metabolism that could be impaired in patients with diabetes and its complications. The aim of our study was to compare MRS of calf muscle between patients with DF and CLI and healthy controls and to evaluate the contribution of MRS in the assessment of the effect of revascularization...
2017: Vnitr̆ní Lékar̆ství
https://www.readbyqxmd.com/read/28516269/%C3%AE-alanine-intercede-metabolic-recovery-for-amelioration-of-human-cervical-and-renal-tumors
#5
Muthuraman Pandurangan, Gansukh Enkhtaivan, Bhupendra Mistry, Rahul V Patel, Sohyun Moon, Doo Hwan Kim
β-Alanine is a non-essential amino acid and presents as a major component of various sports supplements. It is a non-proteogenic amino acid, formed in vivo by degradation of carnosine, anserine, balenine, and dihydrouracil. The present study was aimed at investigating the anti-tumor effects of β-alanine in renal and cervical tumor cells. Sulforhodamine-B assay and flow cytometric analysis were used to measure cell viability. Lactate dehydrogenase (LDH) expression was analyzed using FITC-conjugated fluorescent antibody...
May 17, 2017: Amino Acids
https://www.readbyqxmd.com/read/28512177/evaluation-of-novel-process-indicators-for-rapid-monitoring-of-hydrogen-peroxide-decontamination-processes
#6
Neil P McLeod, Melanie Clifford, J Mark Sutton
Geobacillus stearothermophilus spores on stainless steel discs are routinely used as Biological Indicators (BIs) for the validation of hydrogen peroxide bio-decontamination processes. Given ongoing concerns about the reliability and response time of BIs, we explored the potential for an enzyme-based approach decontamination process evaluation. Thermostable adenylate kinase (tAK) enzyme was coated onto a solid support and exposed to hydrogen peroxide vapour, in parallel with standard commercial 6-log BIs, during a series of vapour-phase hydrogen peroxide (VPHP) cycles in a flexible film isolator...
May 15, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/28507727/correlation-between-hemoglobin-a1c-and-serum-lipid-profile-in-afghani-patients-with-type-2-diabetes-hemoglobin-a1c-prognosticates-dyslipidemia
#7
Arshad Hussain, Iftikhar Ali, Muhammad Ijaz, Afaq Rahim
BACKGROUND: Patients with type 2 diabetes (T2DM) have an increased prevalence of dyslipidemia, which contributes to their high risk of cardiovascular diseases (CVDs). This study is an attempt to determine the correlation between hemoglobin A1c (HbA1c) and serum lipid profile and to evaluate the importance of HbA1c as an indicator of dyslipidemia in Afghani patients with T2DM. METHODS: A total of 401 Afghani patients with T2DM (men, 175; women, 226; mean age, 51...
April 2017: Therapeutic Advances in Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28503498/evaluation-of-energy-balance-on-human-telomerase-reverse-transcriptase-htert-alternative-splicing-by-semi-quantitative-rt-pcr-in-human-umbilical-vein-endothelial-cells
#8
Mohaddeseh Behjati, Mohammad Hashemi, Mohammad Kazemi, Mansoor Salehi, Shaghayegh Haghjooy Javanmard
BACKGROUND: Decreased high-energy phosphate level is involved in endothelial cell injury and dysfunction. Reduced telomerase activity in endothelial cells in parallel with reduced energy levels might be due to altered direction of alternative splicing machine as a complication of depleted energy during the process of atherosclerosis. MATERIALS AND METHODS: Isolated human umbilical vein endothelial cells (HUVECs) were treated for 24 hours by oligomycine (OM) and 2-deoxy glucose (2-DG)...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28503167/the-impact-of-space-flight-on-survival-and-interaction-of-cupriavidus-metallidurans-ch34-with-basalt-a-volcanic-moon-analog-rock
#9
Bo Byloos, Ilse Coninx, Olivier Van Hoey, Charles Cockell, Natasha Nicholson, Vyacheslav Ilyin, Rob Van Houdt, Nico Boon, Natalie Leys
Microbe-mineral interactions have become of interest for space exploration as microorganisms could be used to biomine from extra-terrestrial material and extract elements useful as micronutrients in life support systems. This research aimed to identify the impact of space flight on the long-term survival of Cupriavidus metallidurans CH34 in mineral water and the interaction with basalt, a lunar-type rock in preparation for the ESA spaceflight experiment, BIOROCK. Therefore, C. metallidurans CH34 cells were suspended in mineral water supplemented with or without crushed basalt and send for 3 months on board the Russian FOTON-M4 capsule...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28502309/effects-of-vitrification-of-cumulus-enclosed-porcine-oocytes-at-the-germinal-vesicle-stage-on-cumulus-expansion-nuclear-progression-and-cytoplasmic-maturation
#10
Ruth Appeltant, Tamás Somfai, Elisa C S Santos, Thanh Quang Dang-Nguyen, Takashi Nagai, Kazuhiro Kikuchi
Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus-oocyte complexes (COCs) and control COCs (no cryoprotectant treatment and no cooling), as well as a toxicity control (no cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol and 17...
May 15, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28501647/application-of-optical-action-potentials-in-human-induced-pluripotent-stem-cells-derived-cardiomyocytes-to-predict-drug-induced-cardiac-arrhythmias
#11
H R Lu, M P Hortigon-Vinagre, V Zamora, I Kopljar, A De Bondt, D J Gallacher, G Smith
INTRODUCTION: Human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) are emerging as new and human-relevant source in vitro model for cardiac safety assessment that allow us to investigate a set of 20 reference drugs for predicting cardiac arrhythmogenic liability using optical action potential (oAP) assay. METHODS: Here, we describe our examination of the oAP measurement using a voltage sensitive dye (Di-4-ANEPPS) to predict adverse compound effects using hiPS-CMs and 20 cardioactive reference compounds...
May 10, 2017: Journal of Pharmacological and Toxicological Methods
https://www.readbyqxmd.com/read/28500222/energy-requirements-of-odor-transduction-in-the-chemosensory-cilia-of-olfactory-sensory-neurons-rely-on-oxidative-phosphorylation-and-glycolytic-processing-of-extracellular-glucose
#12
Pablo S Villar, Ricardo Delgado, Cecilia Vergara, Juan G Reyes, Juan Bacigalupo
The mechanisms that power the physiological events occuring in cilia, flagella and microvilli are of fundamental importance for the functions of these important and ubicuous organelles. The olfactory epithelium (OE) is mostly populated by ciliated olfactory sensory neurons (OSNs) and surrounding sustentacular cells (SCs) with apical microvilli. The only OSN dendrite extends to the surface forming a knob projecting several chemosensory cilia of approximately 50 x 0.2 μm, devoid of inner membranes embedded in a mucus layer...
May 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28497358/predictive-value-of-selected-biomarkers-related-to-metabolism-and-oxidative-stress-in-children-with-autism-spectrum-disorder
#13
Afaf El-Ansary, Geir Bjørklund, Salvatore Chirumbolo, Osima M Alnakhli
Autism spectrum disorder (ASD) as a neurodevelopmental disorder is characterized by impairments in social interaction, communication, and restricted, repetitive behavior. Several and reproducible studies have suggested that oxidative stress may represent one of the primary etiological mechanism of ASD that can be targeted for therapeutic intervention. In the present study, multiple regression and combined receiver operating characteristic (ROC) analysis were used to search for a relationship between impaired energy and oxidative metabolic pathways in the etiology of ASD and to find the linear combination that maximizes the partial area under a ROC curve for a pre-identified set of markers related to energy metabolism and oxidative stress...
May 11, 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/28497200/effect-of-lipoic-acid-on-the-biochemical-mechanisms-of-resistance-to-bortezomib-in-sh-sy5y-neuroblastoma-cells
#14
Daniele Tibullo, Cesarina Giallongo, Fabrizio Puglisi, Daniele Tomassoni, Giuseppina Camiolo, Martina Cristaldi, Maria Violetta Brundo, Carmelina Daniela Anfuso, Gabriella Lupo, Tomaso Stampone, Giovanni Li Volti, Francesco Amenta, Roberto Avola, Vincenzo Bramanti
Neuroblastoma (NB) is an extracranial solid cancer and the most common cancer in infancy. Despite the standard treatment for NB is based on the combination of chemotherapeutic drugs such as doxorubicin, vincristine, cyclophosphamide, and cisplatin, chemoresistance occurs over the time. The aim of the present research was to evaluate the effect of bortezomib (BTZ) (50 nM) on NB cell viability and how lipoic acid (ALA) (100 μM) modifies pharmacological response to this chemotherapeutic agent. Cell viability was assessed by ATP luminescence assay whereas expression of oxidative stress marker (i...
May 11, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28495544/modeling-glucose-metabolism-and-lactate-production-in-the-kidney
#15
Ying Chen, Brendan C Fry, Anita T Layton
The metabolism of glucose provides most of the ATP required for energy-dependent transport processes. In the inner medulla of the mammalian kidney, limited blood flow and O2 supply yield low oxygen tension; therefore, a substantial fraction of the glucose metabolism in that region is anaerobic. Lactate is considered to be a waste product of anaerobic glycolysis, which yields two lactate molecules for each glucose molecule consumed, thereby likely leading to the production and accumulation of a significant amount of lactate in the inner medulla...
May 8, 2017: Mathematical Biosciences
https://www.readbyqxmd.com/read/28495375/methylene-blue-stimulates-substrate-level-phosphorylation-catalysed-by-succinyl-coa-ligase-in-the-citric-acid-cycle
#16
T Komlódi, L Tretter
Methylene blue (MB), a potential neuroprotective agent, is efficient in various neurodegenerative disease models. Beneficial effects of MB have been attributed to improvements in mitochondrial functions. Substrate-level phosphorylation (SLP) results in the production of ATP independent from the ATP synthase (ATP-ase). In energetically compromised mitochondria, ATP produced by SLP can prevent the reversal of the adenine nucleotide translocase and thus the hydrolysis of glycolytic ATP. The aim of the present study was to investigate the effect of MB on mitochondrial SLP catalysed by succinyl-CoA ligase...
May 8, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28486967/pseudoxanthoma-elasticum
#17
REVIEW
Dominique P Germain
Pseudoxanthoma elasticum (PXE) is a genetic metabolic disease with autosomal recessive inheritance caused by mutations in the ABCC6 gene. The lack of functional ABCC6 protein leads to ectopic mineralization that is most apparent in the elastic tissues of the skin, eyes and blood vessels. The clinical prevalence of PXE has been estimated at between 1 per 100,000 and 1 per 25,000, with slight female predominance. The first clinical sign of PXE is almost always small yellow papules on the nape and sides of the neck and in flexural areas...
May 10, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/28486587/effects-of-in-vitro-exposure-to-dibutyl-phthalate-mono-butyl-phthalate-and-acetyl-tributyl-citrate-on-ovarian-antral-follicle-growth-and-viability
#18
Lindsay M Rasmussen, Nivedita Sen, Jahaira C Vera, Xiaosong Liu, Zelieann R Craig
Dibutyl phthalate (DBP) is present in consumer products and the coating of some oral medications. Acetyl tributyl citrate (ATBC) has been proposed as an alternative to DBP because DBP causes endocrine disruption in animal models. Following ingestion, DBP is converted to its main metabolite mono-butyl phthalate (MBP) which has been detected in >90% of human follicular fluid samples. Previous studies show that DBP reduces the number of antral follicles present in the ovaries of mice. Thus, this study was designed to evaluate the effects of DBP, MBP, and ATBC on in vitro growth and viability of mouse ovarian antral follicles...
May 8, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28486329/effect-of-short-term-coenzyme-q10-supplementation-and-pre-cooling-on-serum-endogenous-antioxidant-enzymes-of-elite-swimmers
#19
Ali Emami, Asghar Tofighi, Siamak A Rezayi, Behnaz B Gilani
This study aimed to investigate the effect of the use of a two-week pre-cooling strategy and supplementation coenzyme Q10 (CoQ10) on superoxide dismutase (SOD), catalase (CAT), and serum glutathione peroxidase (GPx) in elite, adolescent swimmers during heavy and regular trainings and recording of freestyle swimming. 36 healthy males (mean ± SD; age: 17.5±1.1, body fat content: 14.55±1.75%) were randomly selected and divided into four groups of CoQ10 (300 mg/d), precooling (immersion in the water at 18 ± 0...
May 5, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28483812/coronary-plaque-characterization-in-psoriasis-reveals-high-risk-features-which-improve-following-treatment-in-a-prospective-observational-study
#20
Joseph B Lerman, Aditya A Joshi, Abhishek Chaturvedi, Tsion M Aberra, Amit K Dey, Justin A Rodante, Taufiq Salahuddin, Jonathan H Chung, Anshuma Rana, Heather L Teague, Jashin J Wu, Martin P Playford, Benjamin N Lockshin, Marcus Y Chen, Veit Sandfort, David A Bluemke, Nehal N Mehta
Background -Psoriasis, a chronic inflammatory disease associated with an accelerated risk of MI, provides an ideal human model to study inflammatory atherogenesis in vivo We hypothesized that the increased cardiovascular risk observed in psoriasis would be partially attributable to an elevated subclinical coronary artery disease (CAD) burden composed of non-calcified plaques with high-risk features. However, inadequate efforts have been made to directly measure CAD in this vulnerable population. As such, we sought to compare total (TB) and non-calcified (NCB) coronary plaque burden, and high-risk plaque (HRP) prevalence, between psoriasis patients (n=105), hyperlipidemic patients eligible for statin therapy under NCEP-ATP III guidelines (n=100) who were ~10 years older, and non-psoriasis healthy volunteers (HV) (n=25)...
May 8, 2017: Circulation
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