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https://www.readbyqxmd.com/read/28636017/effect-of-cisplatin-on-the-transport-activity-of-pii-type-atpases
#1
Francesco Tadini-Buoninsegni, Giacomo Sordi, Serena Smeazzetto, Giovanni Natile, Fabio Arnesano
Cisplatin (cis-diamminedichlorido-Pt(ii)) is extensively used as a chemotherapeutic agent against various types of tumors. However, cisplatin administration causes serious side effects, including nephrotoxicity, ototoxicity and neurotoxicity. It has been shown that cisplatin can interact with P-type ATPases, e.g., Cu(+)-ATPases (ATP7A and ATP7B) and Na(+),K(+)-ATPase. Cisplatin-induced inhibition of Na(+),K(+)-ATPase has been related to the nephrotoxic effect of the drug. To investigate the inhibitory effects of cisplatin on the pumping activity of PII-type ATPases, electrical measurements were performed on sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) and Na(+),K(+)-ATPase embedded in vesicles/membrane fragments adsorbed on a solid-supported membrane...
June 21, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28633677/surveillance-of-endoscopes-comparison-of-different-sampling-techniques
#2
Lien Cattoir, Thomas Vanzieleghem, Lisa Florin, Tania Helleputte, Martine De Vos, Bruno Verhasselt, Jerina Boelens, Isabel Leroux-Roels
OBJECTIVE To compare different techniques of endoscope sampling to assess residual bacterial contamination. DESIGN Diagnostic study. SETTING The endoscopy unit of an 1,100-bed university hospital performing ~13,000 endoscopic procedures annually. METHODS In total, 4 sampling techniques, combining flushing fluid with or without a commercial endoscope brush, were compared in an endoscope model. Based on these results, sterile physiological saline flushing with or without PULL THRU brush was selected for evaluation on 40 flexible endoscopes by adenosine triphosphate (ATP) measurement and bacterial culture...
June 21, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28633562/advances-in-imaging-brain-metabolism
#3
Fahmeed Hyder, Douglas L Rothman
Metabolism is central to neuroimaging because it can reveal pathways by which neuronal and glial cells use nutrients to fuel their growth and function. We focus on advanced magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) methods used in brain metabolic studies. (17)O-MRS and (31)P-MRS, respectively, provide rates of oxygen use and ATP synthesis inside mitochondria, whereas (19)F-MRS enables measurement of cytosolic glucose metabolism. Calibrated functional MRI (fMRI), an advanced form of fMRI that uses contrast generated by deoxyhemoglobin, provides maps of oxygen use that track neuronal firing across brain regions...
June 21, 2017: Annual Review of Biomedical Engineering
https://www.readbyqxmd.com/read/28632902/impaired-hippocampal-glucose-metabolism-during-and-after-flurothyl-induced-seizures-in-mice-reduced-phosphorylation-coincides-with-reduced-activity-of-pyruvate-dehydrogenase
#4
Tanya S McDonald, Karin Borges
OBJECTIVE: To determine changes in glucose metabolism and the enzymes involved in the hippocampus ictally and postictally in the acute mouse flurothyl seizure model. METHODS: [U-(13) C]-Glucose was injected (i.p.) prior to, or following a 5 min flurothyl-induced seizure. Fifteen minutes later, mice were killed and the total metabolite levels and % (13) C enrichment were analyzed in the hippocampal formation using gas chromatography-mass spectrometry. Activities of key metabolic and antioxidant enzymes and the phosphorylation status of pyruvate dehydrogenase were measured, along with lipid peroxidation...
June 20, 2017: Epilepsia
https://www.readbyqxmd.com/read/28631948/possible-contribution-of-pannexin-1-to-capsaicin-induced-atp-release-in-rat-nasal-columnar-epithelial-cells
#5
Toyoaki Ohbuchi, Ba Hung Do, Hiroki Koizumi, Shoko Takeuchi, Yoichi Ueta, Hideaki Suzuki
Current evidence indicates that transient receptor potential (TRP) channel activity involves a relationship between opening of pannexin-1 and release of ATP into the extracellular space. We examined the effects of agonists of thermosensitive TRP channels (TRPM8, TRPA1, TRPV1, and TRPV2) on ATP release from rat nasal mucosa, and measured ciliary beat frequency (CBF) using digital high-speed video imaging. Single-cell patch clamping from dissociated rat nasal columnar epithelial cells was performed to confirm the relationship between pannexin-1 and TRP...
February 10, 2017: Channels
https://www.readbyqxmd.com/read/28631388/the-efficacy-of-commercial-tooth-storage-media-for-maintaining-the-viability-of-human-periodontal-ligament-fibroblasts
#6
W Lee, S Stover, M Rasoulianboroujeni, K Sherman, F Fahimipour, E Dashtimoghadam, C Zito, H Jazayeri, L Tayebi
AIM: To evaluate Save-A-Tooth (SAT), EMT Toothsaver (EMT), and Hank's Balanced Salt Solution (HBSS) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblast (HPDLF). METHODOLOGY: Primary HPDLFs were seeded on 96-well cell culture plates and exposed to SAT, EMT, HBSS and water (negative control) for 0.5, 1, 3, 6, 12 and 24 hours at room temperature (22°C). After each exposure time, cell viability was measured by quantifying adenosine triphosphate (ATP) using a luminescent dye...
June 20, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28630044/single-molecule-fret-analysis-discloses-the-dynamics-of-the-dna-topoisomerase-ii-top2-interaction-in-the-presence-of-top2-targeting-agents
#7
Wan-Chen Huang, Chun-Ying Lee, Tao-Shih Hsieh
Topoisomerases play crucial roles in DNA replication, transcription, and recombination. For instance, topoisomerase II (Top2) is critically important for resolving DNA tangles during cell division, and as such, it is a broad anticancer drug target. Top2 regulates DNA topology by transiently breaking one double stranded DNA molecule (cleavage), allowing a second double strand to pass through the opened DNA gate (opening), and then closing the gate by rejoining the broken ends. Drugs that modulate Top2 catalysis may therefore affect enzymatic activity at several different steps...
June 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28628302/highly-fe3-selective-fluorescent-nanoprobe-based-on-ultra-bright-n-p-co-doped-carbon-dots-and-its-application-in-biological-samples
#8
Jingfang Shangguan, Jin Huang, Dinggeng He, Xiaoxiao He, Kemin Wang, Runzhi Ye, Xue Yang, Taiping Qing, Jinlu Tang
Measuring the levels of Fe3+ in human body has attracted considerable attention for health monitoring as it plays an essential element in many physiological processes. In this work, we reported a selective fluorescent nanoprobe for Fe3+ detection in biological samples based on ultra-bright N/P co-doped carbon dots. By employing adenosine 5'-triphosphate (ATP) as the carbon, nitrogen and phosphorus source, the N/P co-doped carbon dots could be simply prepared through hydrothermal treatment. The obtained carbon dots exhibited high quantum yields up to 43...
June 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28624939/additive-effect-of-calcium-depletion-and-low-resource-quality-on-gammarus-fossarum-crustacea-amphipoda-life-history-traits
#9
Marc Rollin, Romain Coulaud, Michael Danger, Bénédicte Sohm, Justine Flayac, Alexandre Bec, Arnaud Chaumot, Olivier Geffard, Vincent Felten
Gammarus fossarum is an often-abundant crustacean detritivore that contributes importantly to leaf litter breakdown in oligotrophic, mainly heterotrophic, headwater streams. This species requires large amounts of Ca to moult, thus allowing growth and reproduction. Because resource quality is tightly coupled to the organism's growth and physiological status, we hypothesised that low Ca concentration [Ca] and low food resource quality (low phosphorus [P] and/or reduced highly unsaturated fatty acid [HUFA] contents) would interactively impair molecular responses (gene expression) and reproduction of G...
June 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28624434/brain-glucose-metabolism-role-of-wnt-signaling-in-the-metabolic-impairment-in-alzheimer-s-disease
#10
REVIEW
Pedro Cisternas, Nibaldo C Inestrosa
The brain is an organ that has a high demand for glucose. In the brain, glucose is predominantly used in energy production, with almost 70% of the energy used by neurons. The importance of the energy requirement in neurons is clearly demonstrated by the fact that all neurodegenerative disorders exhibit a critical metabolic impairment that includes decreased glucose uptake/utilization and decreased mitochondrial activity, with a consequent diminution in ATP production. In fact, in Alzheimer's disease, the measurement of the general metabolic rate of the brain has been reported to be an accurate tool for diagnosis...
June 14, 2017: Neuroscience and Biobehavioral Reviews
https://www.readbyqxmd.com/read/28622850/infection-of-porcine-precision-cut-intestinal-slices-by-transmissible-gastroenteritis-coronavirus-demonstrates-the-importance-of-the-spike-protein-for-enterotropism-of-different-virus-strains
#11
Tanja Krimmling, Andreas Beineke, Christel Schwegmann-Weßels
TGEV is a coronavirus that is still widely spread in pig farming. On molecular level this virus has been studied in detail. However, studying TGEV infection within the complexity of the porcine intestinal epithelium reveals difficulties due to limiting infection models. Here we established a new ex vivo model to analyze the enterotropism of TGEV in porcine intestinal tissue. Precision cut intestinal slices (PCIS) were produced and ATP level was measured to proof vitality of the slices. ATP measurements and HE staining revealed living tissue in culture for up to 24h...
June 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28620318/extraction-protocols-for-individual-zebrafish-s-ventricle-myosin-and-skeletal-muscle-actin-for-in-vitro-motility-assays
#12
Lisa-Mareike Scheid, Cornelia Weber, Nasrin Bopp, Matias Mosqueira, Rainer H A Fink
The in vitro motility assay (IVMA) is a technique that enables the measurement of the interaction between actin and myosin providing a relatively simple model to understand the mechanical muscle function. For actin-myosin IVMA, myosin is immobilized in a measurement chamber, where it converts chemical energy provided by ATP hydrolysis into mechanical energy. The result is the movement of fluorescently labeled actin filaments that can be recorded microscopically and analyzed quantitatively. Resulting sliding speeds and patterns help to characterize the underlying actin-myosin interaction that can be affected by different factors such as mutations or active compounds...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28619102/dl-3-n-butylphthalide-protects-the-heart-against-ischemic-injury-and-h9c2-cardiomyoblasts-against-oxidative-stress-involvement-of-mitochondrial-function-and-biogenesis
#13
Xiaochao Tian, Weiliang He, Rong Yang, Yingping Liu
BACKGROUND: Myocardial infarction (MI) is an acute and fatal condition that threatens human health. Dl-3-n-butylphthalide (NBP) has been used for the treatment of acute ischemic stroke. Mitochondria may play a protective role in MI injury. However, there are few reports on the cardioprotective effect of NBP or the potential mitochondrial mechanism for the NBP-induced protection against cardiac ischemia injury. We investigated the therapeutic effects of NBP in an in vivo MI model and an in vitro oxidative stress model, as well as the potential mitochondrial mechanism...
June 15, 2017: Journal of Biomedical Science
https://www.readbyqxmd.com/read/28619042/metabolic-targeting-of-hif-dependent-glycolysis-reduces-lactate-increases-oxygen-consumption-and-enhances-response-to-high-dose-single-fraction-radiotherapy-in-hypoxic-solid-tumors
#14
Eric Leung, Rob A Cairns, Naz Chaudary, Ravi N Vellanki, Tuula Kalliomaki, Eduardo H Moriyama, Hilda Mujcic, Brian C Wilson, Bradly G Wouters, Richard Hill, Michael Milosevic
BACKGROUND: A high rate of glycolysis leading to elevated lactate content has been linked to poor clinical outcomes in patients with head and neck and cervical cancer treated with radiotherapy. Although the biological explanation for this relationship between lactate and treatment response remains unclear, there is a continued interest in evaluating strategies of targeting metabolism to enhance the effectiveness of radiotherapy. The goal of this study was to investigate the effect of metabolic-targeting through HIF-1α inhibition and the associated changes in glycolysis, oxygen consumption and response on the efficacy of high-dose single-fraction radiotherapy (HD-SFRT)...
June 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28618116/differences-in-p53-status-significantly-influence-the-cellular-response-and-cell-survival-to-1-25-dihydroxyvitamin-d3-metformin-cotreatment-in-colorectal-cancer-cells
#15
Mohamed A Abu El Maaty, Wendy Strassburger, Tooba Qaiser, Yasamin Dabiri, Stefan Wölfl
Mutations in the tumor suppressor p53 are highly prevalent in cancers and are known to influence the sensitivity of cells to various chemotherapeutics including the anti-cancer candidates 1,25-dihydrovitamin D3 [1,25D3] and metformin. Previous studies have demonstrated additive/synergistic anti-cancer effects of the 1,25D3-metformin combination in different models, however the influence of p53 status on the efficacy of this regimen has not been investigated. The CRC cell lines HCT116 wild-type (wt), HCT116 p53-/- and HT-29 (mutant; R273H) were employed, covering 3 different p53 variations...
June 15, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28615375/inclusion-of-almonds-in-a-cholesterol-lowering-diet-improves-plasma-hdl-subspecies-and-cholesterol-efflux-to-serum-in-normal-weight-individuals-with-elevated-ldl-cholesterol
#16
Claire E Berryman, Jennifer A Fleming, Penny M Kris-Etherton
Background: Almonds may increase circulating HDL cholesterol when substituted for a high-carbohydrate snack in an isocaloric diet, yet little is known about the effects on HDL biology and function.Objective: The objective was to determine whether incorporating 43 g almonds/d in a cholesterol-lowering diet would improve HDL subspecies and function, which were secondary study outcomes.Methods: In a randomized, 2-period, crossover, controlled-feeding study, a diet with 43 g almonds/d (percentage of total energy: 51% carbohydrate, 16% protein, and 32% total and 8% saturated fat) was compared with a similar diet with an isocaloric muffin substitution (58% carbohydrate, 15% protein, and 26% total and 8% saturated fat) in men and women with elevated LDL cholesterol...
June 14, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28613823/inhibition-of-mitochondrial-bioenergetics-by-esterase-triggered-cos-h2s-donors
#17
Andrea K Steiger, Michela Marcatti, Csaba Szabo, Bartosz Szczesny, Michael D Pluth
Hydrogen sulfide (H2S) is an important biological mediator, and synthetic H2S donating molecules provide an important class of investigative tools for H2S research. Here we report esterase-activated H2S donors that function by first releasing carbonyl sulfide (COS), which is rapidly converted to H2S by the ubiquitous enzyme carbonic anhydrase (CA). We report the synthesis, self-immolative decomposition, and H2S release profiles of the developed scaffolds. In addition, the developed esterase-triggered COS/H2S donors exhibit higher levels of cytotoxicity than equivalent levels of Na2S or the common H2S donors GYY4137 and AP39...
June 14, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28609713/nicorandil-modulated-macrophages-activation-and-polarization-via-nf-%C3%AE%C2%BAb-signaling-pathway
#18
Fengyun Zhang, Yongli Xuan, Jinjin Cui, Xinxin Liu, Zhiying Shao, Bo Yu
Nicorandil, a drug with both nitrate-like and ATP-sensitive potassium (KATP) channel-activating properties, has been well demonstrated in various aspects of myocardial infarction (MI), especially in inhibiting cell apoptosis and increasing coronary flow. However, the role of nicorandil in regulating inflammation and angiogenesis following myocardial infarction is still unrevealed. In the present study, we explored the effect of nicorandil on macrophage phenotype transition and inflammation regulation and the potential underlying mechanisms...
June 10, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28607618/catechin-and-epicatechin-reduce-mitochondrial-dysfunction-and-oxidative-stress-induced-by-amiodarone-in-human-lung-fibroblasts
#19
Luciana Fernandes Silva Santos, Adriana Stolfo, Caroline Calloni, Mirian Salvador
BACKGROUND: Amiodarone (AMD) and its metabolite N-desethylamiodarone can cause some adverse effects, which include pulmonary toxicity. Some studies suggest that mitochondrial dysfunction and oxidative stress may play a role in these adverse effects. Catechin and epicatechin are recognized as important phenolic compounds with the ability to decrease oxidative stress. Therefore, the aim of this study was to evaluate the potential of catechin and epicatechin to modulate mitochondrial dysfunction and oxidative damage caused by AMD in human lung fibroblast cells (MRC-5)...
June 2017: Journal of Arrhythmia
https://www.readbyqxmd.com/read/28607162/impact-of-age-on-the-association-between-cardiac-high-energy-phosphate-metabolism-and-cardiac-power-in-women
#20
Maria Nathania, Kieren G Hollingsworth, Matthew Bates, Christopher Eggett, Michael I Trenell, Lazar Velicki, Petar M Seferovic, Guy A MacGowan, Doug M Turnbull, Djordje G Jakovljevic
OBJECTIVE: Diminished cardiac high-energy phosphate metabolism (phosphocreatine-to-ATP (PCr:ATP) ratio) and cardiac power with age may play an important roles in development of cardiac dysfunction and heart failure. The study defines the impact of age on PCr:ATP ratio and cardiac power and their relationship. METHODS: Thirty-five healthy women (young≤50 years, n=20; and old≥60 years, n=15) underwent cardiac MRI with (31)P spectroscopy to assess PCr:ATP ratio and performed maximal graded cardiopulmonary exercise testing with simultaneous gas-exchange and central haemodynamic measurements...
June 12, 2017: Heart: Official Journal of the British Cardiac Society
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