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https://www.readbyqxmd.com/read/28816611/biosynthesis-regulation-of-natamycin-production-from-streptomyces-natalensis-hdmnte-01-enhanced-by-response-surface-methodology
#1
Jingping Ge, Changli Wang, Shoufeng Huang, Renpeng Du, Kun Liu, Gang Song, Wenxiang Ping
Natamycin has been widely applied in medical treatments and food protection widely due to its effective inhibition to the growth of yeast and mold. As polyene macrolide antibiotic, the biosynthesis pathway of natamycin is relatively clear. To regulate the biosynthesis of natamycin, additions of precursors affecting cell growth and natamycin production were investigated. The results showed that 0.003% (w/v) potassium ferrocyanide and sodium propionate: n-butanol at a ratio of 4:1 was added into the broth at 0 h and 24 h respectively and they contributed to yield natamycin, reaching 1...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28815441/pluripotent-stem-cells-in-adult-tissues-struggling-to-be-acknowledged-over-two-decades
#2
REVIEW
Deepa Bhartiya
Stem cells have fascinated scientists for a long time and huge research efforts have been put into them as they have the potential to regenerate diseased organs. Besides embryonic stem cells (ES) and induced pluripotent stem cells (iPS), it has been postulated that pluripotent stem cells (PSCs) may also exist in various adult tissues. They are thought to be more primitive than the adult stem cells (ASCs), serve as a backup pool to give rise to ASCs and thus play a crucial role in maintaining life-long homeostasis...
August 16, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28812072/critical-universality-and-asymmetry-of-ionic-solution-iodobenzene-1-decyl-3-methyl-imidazolium-bis-trifluorosulfonyl-imide
#3
Xiaoyi Tao, Tianxiang Yin, Weiguo Shen
The liquid-liquid coexistence curve, heat capacity in the critical and noncritical regions, and the turbidity in critical one-phase and two-phase regions of the binary solution {iodobenzene + 1-decyl-3-methylimidazolium bis(trifluorosulfonyl)imide ([C10mim][NTf2])} have been precisely measured. From the data collected in the critical region, the critical exponents α, β, γ and ν, as well as the universal critical amplitude ratios RB and X were obtained and were shown to agree well with their theoretical values for the 3D-Ising universality class, which further confirmed the 3D-Ising criticality of ionic solutions even in a very low relative permittivity solvent...
August 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28808820/benzene-contaminated-groundwater-remediation-using-calcium-peroxide-nanoparticles-synthesis-and-process-optimization
#4
Hamid Mosmeri, Ebrahim Alaie, Mahmoud Shavandi, Seyed Mohammad Mehdi Dastgheib, Saeideh Tasharrofi
Nano-size calcium peroxide (nCaO2) is an appropriate oxygen source which can meet the needs of in situ chemical oxidation (ISCO) for contaminant remediation from groundwater. In the present study, an easy to handle procedure for synthesis of CaO2 nanoparticles has been investigated. Modeling and optimization of synthesis process was performed by application of response surface methodology (RSM) and central composite rotatable design (CCRD) method. Synthesized nanoparticles were characterized by XRD and FESEM techniques...
August 14, 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28808639/comparison-of-cyclic-fatigue-life-of-nickel-titanium-files-an-examination-using-high-speed-camera
#5
Taha Özyürek, Neslihan Büşra Keskin, Fatma Furuncuoğlu, Uğur İnan
OBJECTIVES: To determine the actual revolutions per minute (rpm) values and compare the cyclic fatigue life of Reciproc (RPC, VDW GmbH), WaveOne (WO, Dentsply Maillefer), and TF Adaptive (TFA, Axis/SybronEndo) nickel-titanium (NiTi) file systems using high-speed camera. MATERIALS AND METHODS: Twenty RPC R25 (25/0.08), 20 WO Primary (25/0.08), and 20 TFA ML 1 (25/0.08) files were employed in the present study. The cyclic fatigue tests were performed using a dynamic cyclic fatigue testing device, which has an artificial stainless steel canal with a 60° angle of curvature and a 5-mm radius of curvature...
August 2017: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/28808387/new-apigenin-glycoside-polyphenolic-constituents-anti-inflammatory-and-hepatoprotective-activities-of-gaillardia-grandiflora-and-gaillardia-pulchella-aerial-parts
#6
Fatma A Moharram, Rabab Abd El Moneim El Dib, Mohamed S Marzouk, Siham M El-Shenawy, Haitham A Ibrahim
BACKGROUND: Gaillardia grandiflora Hort. ex Van Houte and Gaillardia pulchella Foug are flowering plants widely cultivated in Egypt for their ornamental value. Previous reports demonstrated that sesquiterpene derivatives represent the major compounds in both species. Moreover, only few flavones were identified from genus Gaillardia and few studies on the cytotoxicity of G. pulchella were found. AIM OF THE STUDY: Investigation of the phenolic constituents of the aerial parts of both species and evaluation of their anti-inflammatory and hepatoprotective activities...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28803348/effect-of-small-proportion-of-butanol-additive-on-the-performance-emission-and-combustion-of-australian-native-first-and-second-generation-biodiesel-in-a-diesel-engine
#7
Md Mofijur Rahman, Mohammad Golam Rasul, Nur Md Sayeed Hassan, Abul Kalam Azad, Md Nasir Uddin
This paper aims to investigate the effect of the addition of 5% alcohol (butanol) with biodiesel-diesel blends on the performance, emissions, and combustion of a naturally aspirated four stroke multi-cylinder diesel engine at different engine speeds (1200 to 2400 rpm) under full load conditions. Three types of local Australian biodiesel, namely macadamia biodiesel (MB), rice bran biodiesel (RB), and waste cooking oil biodiesel (WCB), were used for this study, and the data was compared with results for conventional diesel fuel (B0)...
August 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28799143/adsorption-studies-on-the-removal-of-cod-and-bod-from-treated-sewage-using-activated-carbon-prepared-from-date-palm-waste
#8
Abd ElAziz A Nayl, Reda A Elkhashab, Tamer El Malah, Sobhy M Yakout, Mohamed A El-Khateeb, Mahmoud M S Ali, Hazim M Ali
In this work, the adsorption of chemical oxygen demand (COD) and biological oxygen demand (BOD) from treated sewage with low-cost activated carbon prepared from date palm shell waste by chemical activation method was studied. Different parameters affecting the adsorption process such as carbon dose, pH, contact time, agitation rate, and temperature were studied. Adsorption equilibrium was attained after 150 min at pH 6.0 with agitation rate of 400 rpm at 25 °C. The results showed that COD removal percentage of 95...
August 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28794553/vitamin-d-level-in-alopecia-areata
#9
Yasmeen Jabeen Bhat, Insha Latif, Rauf Malik, Iffat Hassan, Gousia Sheikh, Kouser Sideeq Lone, Sabiya Majeed, Peerzada Sajad
BACKGROUND: Alopecia areata (AA) is an immune-mediated disease in which autoantigens play an important part in activating T-lymphocytes. Vitamin D has been associated with various autoimmune diseases, and Vitamin D receptors are strongly expressed in hair follicles and their expression in keratinocytes is necessary for the maintenance of the normal hair cycle. AIM: The aim of this study was to find the association between Vitamin D level and AA. MATERIALS AND METHODS: This was a hospital-based cross-sectional study in which 50 patients with clinically and trichoscopically diagnosed AA cases, and 35 healthy age- and sex-matched controls were studied in summer months...
July 2017: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/28794310/structured-mono-and-diacylglycerols-with-a-high-content-of-medium-chain-fatty-acids
#10
Alaina A Esperón-Rojas, R Baeza-Jiménez, Cynthia Cano-Sarmiento, Hugo S García
In the present work, direct enzyme-catalysed esterification of medium chain fatty acids (MCFA) from three different sources (Medium chain triacylglycerols, MCT; saponified MCT and a mixture of free MCFA) was evaluated to obtain structured mono- and diacylglycerols. The esterifications were carried out mixing the different sources of MCFA with glycerol at two weight ratios (1:1 and 4:1, w/w), using three immobilized lipases: Novozym 435, Lipozyme RM IM and Lipozyme TL IM; different reaction times (t = 0, 15, 30, 60, 120 min); enzyme loadings (5, 10 or 15% with respect to the total weight of substrates)...
August 8, 2017: Journal of Oleo Science
https://www.readbyqxmd.com/read/28793754/necessity-of-purification-during-bacterial-dna-extraction-with-environmental-soils
#11
Hyun Jeong Lim, Jung-Hyun Choi, Ahjeong Son
Objectives: Complexity and heterogeneity of soil samples have often implied the inclusion of purification steps in conventional DNA extraction for PCR assays. Unfortunately the purification steps are also time and labor intensive. Therefore the necessity of DNA purification was re-visited and investigated for a variety of environmental soil samples that contained various amounts of PCR inhibitors. Methods: Bead beating and centrifugation was used as the baseline (without purification) method for DNA extraction...
August 8, 2017: Environmental Health and Toxicology
https://www.readbyqxmd.com/read/28783308/rotating-sleeve-triboelectric-electromagnetic-hybrid-nanogenerator-for-high-efficiency-of-harvesting-mechanical-energy
#12
Ran Cao, Tao Zhou, Bin Wang, Yingying Yin, Zuqing Yuan, Congju Li, Zhong Lin Wang
Currently, a triboelectric nanogenerator (TENG) and an electromagnetic generator (EMG) have been hybridized to effectively scavenge mechanical energy. However, one critical issue of the hybrid device is the limited output power due to the mismatched output impedance between the two generators. In this work, impedance matching between the TENG and EMG is achieved facilely through commercial transformers, and we put forward a highly integrated hybrid device. The rotating-sleeve triboelectric-electromagnetic hybrid nanogenerator (RSHG) is designed by simulating the structure of a common EMG, which ensures a high efficiency in transferring ambient mechanical energy into electric power...
August 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/28779906/-reverse-expansion-a-new-technique-of-breast-reconstruction-with-autologous-tissue
#13
L Fabiocchi, G Semprini, F Cattin, L Dellachiesa, T Fogacci, G Frisoni, D Samorani
The treatment for breast cancer is sometimes long and requires a multidisciplinary approach. In 2010, in our centre, we began to perform fat grafting for breast reconstruction using the so-called 'reverse expansion' technique. This consists of the insertion of a skin expander during mastectomy, in its expansion and then in its gradual deflation in the surgical theatre during fat grafting. We performed a complete breast reconstruction in 57 patients by reverse expansion. We harvested fat from the fat excess areas using a normal liposuction cannula...
July 18, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28777391/design-and-numerical-evaluation-of-an-axial-partial-assist-blood-pump-for-chinese-and-other-heart-failure-patients
#14
Guang-Mao Liu, Dong-Hai Jin, Jian-Ye Zhou, Xi-Hang Jiang, Han-Song Sun, Yan Zhang, Hai-Bo Chen, Sheng-Shou Hu, Xing-Min Gui
A fully implantable axial left ventricular assist device LAP31 was developed for Chinese or other heart failure patients who need partial support. Based on the 5-Lpm total cardiac blood output of Chinese without heart failure disease, the design point of LAP31 was set to a flow rate of 3 Lpm with 100-mmHg pressure head. To achieve the required pressure head and good hemolytic performance, a structure that includes a spindly rotor hub and a diffuser with splitter and cantilevered main blades was developed. Computational fluid dynamics (CFD) was used to analyze the hydraulic and hemodynamic performance of LAP31...
August 2, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28776492/pinaverium-bromide-development-and-validation-of-spectrophotometric-methods-for-assay-and-dissolution-studies
#15
Danielly da Fonte Carvalho Martins, Lorena Coimbra Florindo, Anna Karolina Mouzer da Silva Machado, Vítor Todeschini, Maximiliano da Silva Sangoi
This study presents the development and validation of UV spectrophotometric methods for the determination of pinaverium bromide (PB) in tablet assay and dissolution studies. The methods were satisfactorily validated according to International Conference on Harmonization guidelines. The response was linear (r² > 0.99) in the concentration ranges of 2-14 μg/mL at 213 nm and 10-70 μg/mL at 243 nm. The LOD and LOQ were 0.39 and 1.31 μg/mL, respectively, at 213 nm. For the 243 nm method, the LOD and LOQ were 2...
August 4, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28771562/measurement-of-surface-roughness-changes-of-unpolished-and-polished-enamel-following-erosion
#16
Francesca Mullan, Rupert S Austin, Charles R Parkinson, Adam Hasan, David W Bartlett
OBJECTIVES: To determine if Sa roughness data from measuring one central location of unpolished and polished enamel were representative of the overall surfaces before and after erosion. METHODS: Twenty human enamel sections (4x4 mm) were embedded in bis-acryl composite and randomised to either a native or polishing enamel preparation protocol. Enamel samples were subjected to an acid challenge (15 minutes 100 mL orange juice, pH 3.2, titratable acidity 41.3mmol OH/L, 62...
2017: PloS One
https://www.readbyqxmd.com/read/28763254/decolorization-of-textile-dyes-in-an-air-lift-bioreactor-inoculated-with-bjerkandera-adusta-obr105
#17
Hong Sodaneath, Jung-In Lee, Seung-Ok Yang, Hyekyeng Jung, Hee Wook Ryu, Kyung-Suk Cho
A new decolorizing white-rot fungus, OBR105, was isolated from Mount Odae in South Korea and identified by the morphological characterization of its fruit body and spores and partial 18s rDNA sequences. The ligninolytic enzyme activity of OBR105 was studied to characterize their decolorizing mechanism using a spectrophotometric enzyme assay. For the evaluation of the decolorization capacity of OBR105, the isolate was incubated in an erlenmeyer flask and in an airlifte bioreator with potato dextrose broth (PDB) medium supplemented with each dye...
August 1, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28759988/comparative-analysis-of-stirred-catalytic-basket-bio-reactor-for-the-production-of-bio-ethanol-using-free-and-immobilized-saccharomyces-cerevisiae-cells
#18
Amir Hussain, Martin Kangwa, Marcelo Fernandez-Lahore
The successful industrial production of ethanol and fine chemicals requires the development of new biocatalytic reactors and support materials to achieve economically viable processes. In this work, a Stirred-Catalytic-Basket-BioReactor using various immobilizing foams as support material and compared to free cells were used, focusing mainly on; (i) effect of mass-transfer on cells physiology and (ii) ethanol productivity. The performance of the reactor was further evaluated by ethanol volumetric productivity, yield and time for process completion and it was found that the variation of ethanol production and diffusion of the substrate in fermentation process are co-related with the stirrer speed and initial glucose concentration...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28759452/influence-of-nanoparticles-on-filterability-of-fruit-juice-industry-wastewater-using-submerged-membrane-bioreactor
#19
Guler Turkoglu Demirkol, Nadir Dizge, Turkan Ormanci Acar, Oyku Mutlu Salmanli, Nese Tufekci
In this study, polyethersulfone (PES) ultrafiltration membrane surface was modified with nano-sized zinc oxide (nZnO) and silver (nAg) to improve the membrane filterability of the mixed liquor and used to treat fruit-juice industry wastewater in a submerged membrane bioreactor (MBR). The nAg was synthesized using three different methods. In the first method, named as nAg-M1, PES membrane was placed on the membrane module and nAg solution was passed through the membrane for 24 h at 25 ± 1 °C. In the second method, named as nAg-M2, PES membrane was placed in a glass container and it was shaken for 24 h at 150 rpm at 25 ± 1 °C...
July 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28758518/allometric-scaling-of-power-force-velocity-ergometry-profiles-in-men
#20
Hamdi Jaafar
AIM: To examine the appropriate magnitude of allometric scaling of the force-velocity relationship according to body dimensions and to establish normative data for the power-force-velocity relationship for active men. SUBJECTS AND METHODS: Ninety-seven participants completed a force-velocity test on a Monark cycle ergometer. Allometric exponents and percentile ranks were established for maximal power (Pmax), maximal force (F0) and maximal velocity (V0). RESULTS: The mean (± SD) of Pmax, F0 and V0 were 1114...
July 31, 2017: Annals of Human Biology
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