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Hossein Shahinian, Mohammed Hassan, Harish Cherukuri, Brigid A Mullany
This paper details the feasibility of using fiber-based tools in a computer numerical control (CNC) environment to process optical materials and their ability to reduce the amplitude of pre-existing mid-spatial-frequency (MSF) surface errors. The work is motivated by earlier research conducted by the group exploring the ability of polymeric fiber-based tools to remove material from BK7 glass substrates. To evaluate these tools in a CNC environment, three tasks are explored. First, the ability of the tools to maintain their form and material removal profile while operating under translational conditions is explored...
October 10, 2017: Applied Optics
Suja Thangaraj, Nagarajan Govindan
The significance of mileage to the fruitful operation of a trucking organization cannot be downplayed. Fuel is one of the biggest variable expenses in a trucking wander. An attempt is made in this research to improve the combustion efficiency of a diesel engine for better fuel economy by introducing hydroxy gas which is also called browns gas or HHO gas in the suction line, without compromising performance and emission. Brown's gas facilitates the air-fuel mixture to ignite faster and efficient combustion. By considering safety and handling issues in automobiles, HHO gas generation by electrolysis of water in the presence of sodium bicarbonate electrolytes (NaHCO3) and usage was explored in this research work over compressed pure hydrogen, due to generation and capacity of immaculate hydrogen as of now confines the application in diesel engine operation...
October 18, 2017: Environmental Science and Pollution Research International
Ana S Fernandes, Susana M Paixão, Tiago P Silva, José C Roseiro, Luís Alves
With the increasing awareness on the toxicity of several synthetic dyes, demand for pigments from natural sources, such as microbial carotenoids, has gained interest as a promising safe alternative colour additive. In this study, a surface response methodology based on the Doehlert distribution for two factors [% of glucose in a mixture of glucose + fructose (10 g/L total sugars), and sulfate concentration] was used towards the optimal carotenoids production by Gordonia alkanivorans strain 1B in the presence of light (400 lx)...
October 17, 2017: Bioprocess and Biosystems Engineering
Jianzhi Zhang, Xi Li
OBJECTIVES: To enhance the efficiency of phenyllactic acid (PLA) production from L-phenylalanine (L-Phe) by introducing a novel artificial pathway into Escherichia coli RESULTS: The production of PLA from L-Phe by recombinant E. coli (ldh-lpox) coexpressing L-phenylalanine oxidase and L-lactate dehydrogenase was studied. The new PLA synthesis pathway was confirmed to be efficient in recombinant E. coli. Subsequently, two different biocatalyst processes were carried out and optimized for PLA production...
October 16, 2017: Biotechnology Letters
Zi-Long Zhao, Shiwei Zhou, Shanqing Xu, Xi-Qiao Feng, Yi Min Xie
A common spinning toy, called "buzzer", consists of a perforated disk and flexible threads. Despite of its simple construction, a buzzer can effectively transfer translational motions into high-speed rotations. In the present work, we find that the disk can be spun by hand at an extremely high rotational speed, e.g., 200,000 rpm, which is much faster than the previously reported speed of any manually operated device. We explore, both experimentally and theoretically, the detailed mechanics and potential applications of such a thread-disk system...
October 13, 2017: Scientific Reports
Mingge Ding, Jiao Ning, Na Feng, Zeyang Li, Zhenhua Liu, Yuanbo Wang, Yueming Wang, Xing Li, Cong Huo, Xin Jia, Rong Xu, Feng Fu, Xiaoming Wang, Jianming Pei
Mechanical trauma (MT) causes myocardial injury and cardiac dysfunction. However, the underlying mechanism remains largely unclear. This study investigated the role of mitochondrial dynamics in post-traumatic cardiac dysfunction and the protective effects of melatonin. Adult male Sprague Dawley rats were subjected to 5-minute rotations (200 revolutions at a rate of 40 rpm) to induce MT model. Melatonin was administrated intraperitoneally 5 minute after MT. Mitochondrial morphology, myocardial injury, and cardiac function were determined in vivo...
October 11, 2017: Journal of Pineal Research
Lagzouli Mohamed, Youssfi El Kettani, Aitounejjar Ali, Elyachioui Mohamed, Jadal Mohamed
OBJECTIVE: Glucoamylase is among the most important enzymes in biotechnology. The present study aims to determine better conditions for growth and glucoamylase production by Candida guilliermondii and to reduce the overall cost of the medium using Box-Behnken design with one central point and response surface methodology. METHODOLOGY: Box-Behnken factorial design based on three levels was carried out to obtain optimal medium combination of five independent variables such as initial pH, soluble starch, CH4N2O, yeast extract and MgSO4...
2017: Pakistan Journal of Biological Sciences: PJBS
Ulisses M F de Oliveira, Leonardo J B Lima de Matos, Maria Cristiane M de Souza, Bruna B Pinheiro, José C S Dos Santos, Luciana R B Gonçalves
Lipase from Rhizomucor miehei (RML) was immobilized onto chitosan support in the presence of some surfactants added at low levels using two different strategies. In the first approach, the enzyme was immobilized in the presence of surfactants on chitosan supports previously functionalized with glutaraldehyde. In the second one, after prior enzyme adsorption on chitosan beads in the presence of surfactants, the complex chitosan beads-enzyme was then cross-linked with glutaraldehyde. The effects of surfactant concentrations on the activities of free and immobilized RML were evaluated...
October 10, 2017: Applied Biochemistry and Biotechnology
Xiao Shen, Danijel Dojcinovic, Lucia Baldi, David L Hacker, Immanuel F Luescher, Florian M Wurm
OBJECTIVES: To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line. RESULTS: When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recombinant major histocompatibility (MHC) class II protein (HLA-DR12xHis) increased four-fold over the levels observed for the same cells cultivated in roller bottles (RB) without vented caps...
October 9, 2017: Biotechnology Letters
Ming Zhang, Pascal Guiraud
The treatment of nanoparticle (NP) polluted aqueous suspensions by flotation can be problematic due to the low probability of collision between particles and bubbles. To overcome this limitation, the present work focuses on developing an enhanced flotation technique using the surface-functionalized microbubbles - colloidal gas aphrons (CGAs). The CGA generator was adapted to be air flow rate controlled based on the classical Sebba system; thus it could be well adopted in a continuous flotation process. Cetyl trimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) were employed for CGA creation...
September 28, 2017: Water Research
Ricardo Andres Landazuri-Del Barrio, Wagner Nunes de Paula, Rubens Spin-Neto, João Antônio Chaves de Souza, Guilherme José Pimentel Lopes de Oliveira, Elcio Marcantonio-Junior
OBJECTIVE: The aim of this study was to evaluate the influence of the drilling speed on bone healing and the osseointegration of implants placed with a guided flapless surgical technique in rabbit tibias. METHODS: For the evaluation of bone healing, a total of 30 perforations (defects) were made in both tibias of 15 rabbits using 2 different drilling speeds (1500 rpm-control group; 50 rpm-test group). The regeneration of bone tissue in the surgical sites was evaluated at 0, 7, and 14 days...
October 3, 2017: Implant Dentistry
B C Saha, G J Kennedy
Itaconic acid (IA), an unsaturated 5-carbon dicarboxylic acid, is a building block platform chemical that is currently produced industrially from glucose by fermentation with Aspergillus terreus. Softwood has the potential to serve as low cost source of sugars for its production. Effective utilization of all softwood derived sugars such as glucose, mannose and galactose by the fungus for production of IA will lower the cost of its production. In this work, 20 A. terreus strains were evaluated for the first time for IA production from mannose and galactose in shake-flasks at initial pH of 3...
October 4, 2017: Letters in Applied Microbiology
Shweta V Pawar, Virendra K Rathod
This study explores a novel concept of co-production of uricase and alkaline protease by Bacillus licheniformis using single substrate in single step. Seven local bacterial strains were screened for uricase production amongst which Bacillus licheniformis is found to produce highest uricase along with alkaline protease. Optimization of various factors influencing maximum enzyme co-production by Bacillus licheniformis are investigated. Maximum enzyme productivity of 0.386 U/mL uricase and 0.507 U/mL alkaline protease is obtained at 8 h incubation period, 1% (v/v) inoculum and at 0...
October 4, 2017: Preparative Biochemistry & Biotechnology
Łukasz Wajda, Aleksandra Duda-Chodak, Tomasz Tarko, Paweł Kamiński
Current study was focused on optimising lead(II) biosorption carried out by living cells of Arthrospira platensis using Principal Component Analysis. Various experimental conditions were considered: initial metal concentration (50 and 100 mg/l), solution pH (4.0, 4.5, 5.0, 5.5) and contact time (10, 20, 30, 40, 50 and 60 min) at constant rotary speed 200 rpm. It was found that when the biomass was separated from experimental solutions by the filtration, almost 50% of initial metal dose was removed by the filter paper...
October 3, 2017: World Journal of Microbiology & Biotechnology
Usha Sharma, Krupa Patel, Vandana Shah, Surabhi Sinha, Vishnu Pratap Singh Rathore
INTRODUCTION: Newer Candida species are now becoming increasingly predominant commensal in the oral cavity. AIM: Aim of the study was to identify and compare different Candida species in the oral cavity of Type II diabetic individuals. MATERIALS AND METHODS: The present microbial study was carried out for the duration of three months. Sixty participants were included in the study and divided into two groups of 30 individual each. Group I consisted of patients with Type II diabetes while Group II consisted of healthy individuals without diabetes or any other systemic disease...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
Sergio R Arias, Frederick A Rueggeberg, Donald Mettenburg, Mohamed Sharawy, Stephen Looney, Ranya Elsayed, Mohammed E Elsalanty
STATEMENT OF PROBLEM: Little information is available on the effect of drilling speed on surrounding bone during the removal of an abutment screw fragment. PURPOSE: The purpose of this in vitro study was to compare, in vitro, the peak temperature increase during the removal of fractured abutment screws from implants placed in a porcine mandible, using drilling speeds of 600 or 2000 rpm. MATERIAL AND METHODS: Twenty 4.3×13-mm dental implants were placed in 10 dissected porcine mandibles: 2 implants per mandible, 1 on each side...
September 26, 2017: Journal of Prosthetic Dentistry
Christine Erbe, Craig McPherson
Geotechnical site investigations prior to marine construction typically involve shallow, small-core drilling and standard penetration testing (SPT), during which a small tube is hammered into the ground at the bottom of the borehole. Drilling (120 kW, 83 mm diameter drillbit, 1500 rpm, 16-17 m drill depth in sand and mudstone) and SPT (50 mm diameter test tube, 15 mm wall thickness, 100 kg hammer, 1 m drop height) by a jack-up rig in 7-13 m of water were recorded with a drifting hydrophone at 10-50 m range...
September 2017: Journal of the Acoustical Society of America
Corinne Frère, Katsushi Kobayashi, Claire Dunois, Jean Amiral, Pierre-Emmanuel Morange, Marie-Christine Alessi
BACKGROUND: Light transmission aggregometry (LTA) is considered as the gold standard for testing platelet function in the setting of both platelet disorders suspicion and response to antiplatelet therapy evaluation. LTA requires however specialized equipment, substantial blood sample volumes, is technically challenging and time-consuming. AIM: To evaluate an automated platelet aggregation method performed on a routine coagulation analyzer Sysmex CS-2000i. METHODS: 46 patients presenting a bleeding syndrome and 62 patients with acute coronary syndrome receiving dual antiplatelet therapy were studied in total...
September 29, 2017: Platelets
G Ramanjaneyulu, A Sridevi, P Seshapani, A Ramya, K Dileep Kumar, G Praveen Kumar Reddy, B Rajasekhar Reddy
Growth of Fusarium sp. BVKT R2, a potential isolate of forest soils of Eastern Ghats on birchwood xylan in mineral salts medium (MSM) under un-optimized conditions of 30 °C, pH of 5.0, 150 rpm and inoculum size of 5 agar plugs for 7 days, yielded titer of 1290 U/mL of xylanase (EC The effect of various operating parameters such as different substrates and their concentration, additional carbon and nitrogen sources, incubation temperature, initial pH, agitation and inoculum size on the production of xylanase by Fusarium sp...
October 2017: 3 Biotech
Shraddha Awasthi, Pratap Srivastava, Pardeep Singh, D Tiwary, Pradeep Kumar Mishra
Biodegradation of plastics, which are the potential source of environmental pollution, has received a great deal of attention in the recent years. We aim to screen, identify, and characterize a bacterial strain capable of degrading high-density polyethylene (HDPE). In the present study, we studied HDPE biodegradation using a laboratory isolate, which was identified as Klebsiella pneumoniae CH001 (Accession No MF399051). The HDPE film was characterized by Universal Tensile Machine (UTM), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscope (SEM), and Atomic Force Microscope (AFM) before and after microbial incubation...
October 2017: 3 Biotech
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