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Shuang Hu, Xiao Yang, Jiao Xue, Xuan Chen, Xiao-Hong Bai, Zhi-Hui Yu
A novel graphene/dodecanol floating solidification microextraction followed by HPLC with diode-array detection has been developed to extract trace levels of four cinnamic acid derivatives in traditional Chinese medicines. Several parameters affecting the performance were investigated and optimized. Also, possible microextraction mechanism was analyzed and discussed. Under the optimum conditions (amount of graphene in dodecanol: 0.25 mg/mL; volume of extraction phase: 70 μL; pH of sample phase: 3; extraction time: 30( ) min; stirring rate: 1000 rpm; salt amount: 26...
May 17, 2017: Journal of Separation Science
Shuangxi Xie, Xiaodong Wang, Niandong Jiao, Steve Tung, Lianqing Liu
Trapping and transporting microorganisms with intrinsic motility are important tasks for biological, physical, and biomedical applications. However, fast swimming speed makes the manipulation of these organisms an inherently challenging task. In this study, we demonstrated that an optoelectrical technique, namely, optically induced dielectrophoresis (ODEP), could effectively trap and manipulate Chlamydomonas reinhardtii (C. reinhardtii) cells swimming at velocities faster than 100 μm s(-1). Furthermore, live C...
May 17, 2017: Lab on a Chip
Sharmini Sunasee, Kien Tiek Wong, Gooyong Lee, Saravanan Pichiah, Shaliza Ibrahim, Chulhwan Park, Nam Chan Kim, Yeomin Yoon, Min Jang
In this study, bisphenol A (BPA) removal by sonophotocatalysis coupled with commercially available titanium dioxide (TiO2, P25) was assessed in batch tests using energy-based advanced oxidation combining ultrasound (US) and ultraviolet (UV). The kinetics of BPA removal were systematically evaluated by changing operational parameters, such as US frequency and power, mechanical stirring speed, and temperature, but also comparison of single and coupled systems under the optimum US conditions (35 kHz, 50 W, 300 rpm stirring speed, and 20 °C)...
May 16, 2017: Environmental Science and Pollution Research International
Elie-Jacques Fares, Laurie Isacco, Cathriona R Monnard, Jennifer L Miles-Chan, Jean-Pierre Montani, Yves Schutz, Abdul G Dulloo
Standardized approaches to assess human energy expenditure (EE) are well defined at rest and at moderate to high-intensity exercise, but not at light intensity physical activities energetically comparable with those of daily life (i.e., 1.5-4 times the resting EE, i.e., 1.5-4 METs). Our aim was to validate a graded exercise test for assessing the energy cost of low-intensity dynamic work in physically inactive humans, that is, those who habitually do not meet the guidelines for moderate-to-vigorous aerobic physical activity levels...
May 2017: Physiological Reports
M Obadah Al-Chekakie, Haikun Bao, Paul W Jones, Kenneth M Stein, Lucas Marzec, Paul D Varosy, Frederick A Masoudi, Jeptha P Curtis, Joseph G Akar
BACKGROUND: Among patients with implantable defibrillators (ICD), use of remote patient monitoring (RPM) is associated with lower risk of death and rehospitalization. Standard ICD RPM can be supplemented with weight and blood pressure data. It is not known whether standard RPM plus routine weight and blood pressure transmission (RPM+) is associated with better outcomes. METHODS AND RESULTS: RPM+ patients (n=4106) were compared with patients who only transmitted standard ICD RPM data (n=14 183)...
May 2017: Circulation. Cardiovascular Quality and Outcomes
Farha Masood, Maria Abdul-Salam, Tariq Yasin, Abdul Hameed
In this study, the influence of different physicochemical parameters on the yield of polyhydroxyalkanoates (PHAs) produced by Bacillus cereus FA11 is investigated. The physicochemical factors include pH, temperature, time, inoculum size and its age, agitation speed and composition of the glucose rich peptone deficient (GRPD) medium. During two-stage fermentation, B. cereus FA11 produced a significantly high (p < 0.05) yield (80.59% w/w) of PHAs copolymer using GRPD medium containing glucose (15 g/L) and peptone (2 g/L) at pH 7, 30 °C and 150 rpm after 48 h of incubation...
May 2017: 3 Biotech
Wengui Shi, Yanfang Xie, Jinpeng He, Jian Zhou, Yuhai Gao, Wenjun Wei, Nan Ding, Huiping Ma, Cory J Xian, Keming Chen, Jufang Wang
It is well documented that microgravity in space environment leads to bone loss in astronauts. These physiological changes have also been validated by human and animal studies and modeled in cell-based analogs. However, the underlying mechanisms are elusive. In the current study, we identified a novel phenomenon that primary cilia (key sensors and functioning organelles) of rat calvarial osteoblasts (ROBs) gradually shrank and disappeared almost completely after exposure to simulated microgravity generated by a random positioning machine (RPM)...
May 12, 2017: Scientific Reports
Priti R Pandit, M H Fulekar
Worldwide consumption of hen eggs results in availability of large amount of discarded egg waste particularly egg shells. In the present study, the waste shells were utilized for the synthesis of highly active heterogeneous calcium oxide (CaO) nanocatalyst to transesterify dry biomass into methyl esters (biodiesel). The CaO nanocatalyst was synthesied by calcination-hydration-dehydration technique and fully characterized by infrared spectroscopy, X-ray powder diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), brunauer-emmett-teller (BET) elemental and thermogravimetric analysis...
May 6, 2017: Journal of Environmental Management
Annica A M Andersson, R Andersson, Anette Jonsäll, Jörgen Andersson, Helena Fredriksson
Wheat bran and rye bran are mostly used as animal feed today, but their high content of dietary fiber and bioactive components are beneficial to human health. Increased use of bran as food raw material could therefore be desirable. However, bran mainly contains unextractable dietary fiber and deteriorates the sensory properties of products. Processing by extrusion could increase the extractability of dietary fiber and increase the sensory qualities of bran products. Wheat bran and rye bran were therefore extruded at different levels of moisture content, screw speed and temperature, in order to find the optimal setting for increased extractability of dietary fiber and positive sensory properties...
May 11, 2017: Journal of Food Science
Ran Han, Yaa Asantewaa Kafui Klu, Jinru Chen
This study compared the abilities of selected Salmonella enterica and enterohemorrhagic Escherichia coli (EHEC) strains of fresh produce origin to form biofilms on polystyrene surface and to attach to alfalfa and bean sprouts. Each of the 7 S. enterica and 4 EHEC inocula (2 mL; 10(7) CFU/mL) was placed in 6 different broths in 24-well polystyrene tissue culture plates at 28 °C for 1 to 7 d. Developed biofilms were quantified using the crystal violet binding assay. In a separate experiment, alfalfa and mung bean sprouts (5 g) were exposed to 25 mL inocula (10(7) CFU/mL) of S...
May 10, 2017: Journal of Food Science
Mustafa M Abo Elela, Nermeen A ElKasabgy, Emad B Basalious
Quetiapine fumarate (QF), an anti-schizophrenic drug, suffers from rapid elimination and poor bioavailability due to extensive first-pass effect. Intramuscularly (IM) injected lipospheres were designed to enhance the drug's bioavailability and extend its release. A central composite design was applied to optimize the liposphere preparation by a melt dispersion technique using Compritol® 888 ATO or glyceryl tristearate as lipid component and polyvinyl alcohol as surfactant. Lipospheres were evaluated for their particle size, entrapment efficiency, and in vitro release...
May 10, 2017: AAPS PharmSciTech
Johnson Preethi, Loganathan Chitra, Krishnan Vennila, Sakayanathan Penislusshiyan, Raji Vijayan, Vijayan Sudha, Palanivel Sathishkumar, Kumarasamy Murugesan, Thayumanavan Palvannan
The aqueous extract of various plants like C. sativum (AECS), A. tenella colla (AEAT), S. hispida (AESH) and M. verticillata (AEMV) was studied for its hexavalent chromium (Cr(VI)) reduction property. Even though antioxidant activity was present, AEAT, AESH and AEMV did not reduce Cr(VI). AECS showed rapid and dose-dependent Cr(VI) reduction. The efficient reduction of 50 mg/L of Cr(VI) using AECS was attained in the presence of 250 µg/ml of starting plant material, incubating the reaction mixture at pH 2, 30 °C and agitation at 190 rpm...
May 10, 2017: Environmental Technology
Wenpan Li, Xirui Zhang, Shasha Jing, Xiu Xin, Kang Chen, Dawei Chen, Haiyang Hu
This research focused on optimizing the preparations of pDNA-loaded calcium phosphate nanoparticles by employing a 3-factor, 3-level Box-Behnken design. Results indicated that a Ca/P ratio of 189.56, pH of 7.82, and a stirring speed of 528.83 rpm were the optimum conditions for preparation of the nanoparticles. The size of the optimized CaP/pDNA nanoparticles was 61.3±3.64 nm, with a polydispersity index of 0.341 and an encapsulation efficiency of up to 92.11 %. The optimized CaP/pDNA nanoparticles had a high transfection efficiency and demonstrated good biocompatibility in vitro...
May 5, 2017: Journal of Pharmaceutical Sciences
Govind V Waghmare, Abhishek Chatterji, Virendra K Rathod
This work illustrates the enzymatic synthesis of cinnamyl butyrate by esterification of butyric acid and cinnamyl alcohol. Experiments were performed to study the various operating parameters such as molar ratio, enzyme concentration, temperature, and speed of agitation. Also, the suitable kinetic model for esterification reaction was predicted and the various kinetic parameters were determined. It has been observed that the experimental results agree well with the simulated results obtained by following the ping-pong bi-bi mechanism with dead-end inhibition by both the substrate acid and alcohol...
May 6, 2017: Applied Biochemistry and Biotechnology
Seung-Ok Yang, Hong Sodaneath, Jung-In Lee, Hyekyeng Jung, Jin-Hee Choi, Hee Wook Ryu, Kyung-Suk Cho
The mycoremediation has been considered as a promising method for decolorizing dye wastewater. To explore new bioresource for mycoremediation, a new white-rot fungus that could decolorize various dyes commonly used in textile industries was isolated, and its ligninolytic enzyme activity and decolorization capacity were characterized. The isolated CBR43 was identified as Trametes versicolor based on the morphological properties of its fruit body and spores, as well as through partial 18S rDNA gene sequences...
May 2, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Ralph Beneke, Tobias G J Weber, Renate M Leithäuser
Effects of different cycling cadences (RPM) on metabolic rate, blood lactate concentration (BLC), and reliance on carbohydrate (CHO) defined as the fraction of oxygen uptake used for CHO oxidation (relCHO) are highly individual. Whether this depends on the individually maximal achievable RPM obtained at minimized cycling resistance (RPMmax), is unknown. We tested the hypotheses that 1) the individual freely chosen RPM at an incremental cycle ergometer test (ILT), and 2) relCHO at given BLC levels both depend on RPMmax...
May 1, 2017: International Journal of Sports Physiology and Performance
Anand Kishore Kola, Mallaiah Mekala, Venkat Reddy Goli
In the present investigation, we report that statistical design and optimization of significant variables for the microbial production of citric acid from sucrose in presence of filamentous fungi A. niger NCIM 705. Various combinations of experiments were designed with Central Composite Design (CCD) of Response Surface Methodology (RSM) for the production of citric acid as a function of six variables. The variables are; initial sucrose concentration, initial pH of medium, fermentation temperature, incubation time, stirrer rotational speed, and oxygen flow rate...
June 2017: Data in Brief
Lorena Vidal, Mazaher Ahmadi, Elena Fernández, Tayyebeh Madrakian, Antonio Canals
This study presents a new, user-friendly, cost-effective and portable headspace solid-phase extraction technique based on graphene oxide decorated with iron oxide magnetic nanoparticles as sorbent, located on one end of a small neodymium magnet. Hence, the new headspace solid-phase extraction technique has been called Magnetic Headspace Adsorptive Extraction (Mag-HSAE). In order to assess Mag-HSAE technique applicability to model analytes, some chlorobenzenes were extracted from water samples prior to gas chromatography-mass spectrometry determination...
June 8, 2017: Analytica Chimica Acta
Elias Luiz de Souza, Noeli Sellin, Cintia Marangoni, Ozair Souza
Pseudostem of the Musa cavendishii banana plant was submitted to chemical pretreatments with acid (H2SO4 2%, 120 °C, 15 min) and with alkali (NaOH 3%, 120 °C, 15 min), saccharified by commercial enzymes Novozymes® (Cellic CTec2 and HTec2). The influences of the pretreatments on the degradation of the lignin, cellulose and hemicellulose, porosity of the surface, particle crystallinity, and yield in reducing sugars after saccharification (Y RS), were established. Different concentrations of biomass (70 and 100 g/L in dry matter (dm)), with different physical differences (dry granulated, crushed wet bagasse, and whole pseudostem), were used...
April 28, 2017: Applied Biochemistry and Biotechnology
Shraddha Awasthi, Neha Srivastava, Tripti Singh, D Tiwary, Pradeep Kumar Mishra
Polythene is considered as one of the important object used in daily life. Being versatile in nature and resistant to microbial attack, they effectively cause environmental pollution. In the present study, biodegradation of low-density polyethylene (LDPE) have been performed using fungal lab isolate Rhizopus oryzae NS5. Lab isolate fungal strain capable of adhering to LDPE surface was used for the biodegradation of LDPE. This strain was identified as Rhizopus oryzae NS5 (Accession No. KT160362). Fungal growth was observed on the surface of the polyethylene when cultured in potato dextrose broth at 30 °C and 120 rpm, for 1 month...
May 2017: 3 Biotech
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