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Yun Zhang, Yang Zhang, Jie Gao, Qiuxuan Shen, Zhihui Bai, Xuliang Zhuang, Guoqiang Zhuang
Although Nitrobacter winogradskyi is an important chemoorganotrophic organism for the study of nitrite-oxidizing bacteria (NOB) physiology as well as nitrification, until now, the mixotrophic medium for this organism growth has not been optimized, comprehensively. In this study, we aimed to improve the growth medium of N. winogradskyi using the one-factor-at-a-time (NaNO2 , glycerol, pH) method. In addition, a further experimental design was carried out based on Central Composite Design (CCD) with response surface methodology (RSM)...
June 21, 2018: Letters in Applied Microbiology
Ali Fatehizadeh, Mohammad Mehdi Amin, Bijan Bina, Mohammad Reza Zare, Mohammad Ghasemian, Ensiyeh Taheri
Background: Due to depletion of fossil fuel reserves, global warming, and the need for energy efficiency, the new energy resource is required. For this purpose, hydrogen can be considered as clean and efficient energy carrier. In this study, biohydrogen production from physical pretreated mixed culture was investigated. Materials and Methods: In this study, the optimal conditions for physical pretreatment of mixed culture were investigated for the enrichment of hydrogen-producing bacteria (HPB)...
2018: Advanced Biomedical Research
Chao-Ping Lin, Xiao-Ling Tang, Ren-Chao Zheng, Yu-Guo Zheng
An efficient chemoenzymatic route was developed for synthesis of (S)-α-amino-4-fluorobenzeneacetic acid, a valuable chiral intermediate of Aprepitant, using immobilized penicillin amidase catalyzed kinetic resolution of racemic N-phenylacetyl-4-fluorophenylglycine. The optimum temperature, pH and agitation rate of the reaction were determined to be 40 °C, 9.5 and 300 rpm, respectively. Kinetic resolution of 80 g L-1 N-phenylacetyl-4-fluorophenylglycine by immobilized amidase 20 g L-1 resulted in 49...
June 15, 2018: Bioorganic Chemistry
Heng-Ju Yoon, Jae Young Lee, Tae-Ho Yoon
A millstone (MS) was introduced in the production of large-size few-layer-graphene oxide (FLGO) via true shear exfoliation in order to minimize fragmentation. The MS was constructed with two glass plates, where the top plate was designed to rotate against the stationary bottom plate, thereby generating true shear force. Mildly oxidized graphite (MOG) was used for MS exfoliation in order to obtain both good property and high yield. The rpm of rotation (10, 20, 30, 40, and 50), solution concentration (0.5, 1, and 2 mg/ml), and number of exfoliation (1, 2, and 3) were optimized by measuring the UV-vis absorption, and the effect of oxidation time (30, 60, and 90 min) was studied under the given optimum conditions...
June 20, 2018: Nanoscale Research Letters
Lili Ling, Yawei Feng, Sa Yan, Donglai Pan, Hao Ge, Hao Li, Hexing Li, Zhenfeng Bian
The introduction of piezoelectric field has been proven a promising method to enhance photocatalytic activity by preventing photoelectron-hole recombination. However, the formation of piezoelectric field requires additional mechanical force or high-frequency ultrasonic baths, which limits its potential application in industrial scale. Therefore, it is of great practical significance to design the catalyst which can harvest the discrete energy such as the fluid mechanical energy to form the electric field. Herein, PZT/TiO2 catalyst with a core-shell configuration was prepared by a simple coating method...
June 21, 2018: Environmental Science & Technology
Carolina Aloisio, Micaela Ponce-Ponte, Gladys E Granero, Marcela R Longhi
The aim of this work was to predict the permeability of two model drugs, sulfamerazine (SMR) and indomethacin (INM), and to determine the effect on their apparent permeabilities by complexation with cyclodextrins and/or meglumine or incorporation in microemulsions. Permeation experiments were performed using two-chamber diffusion cells with a new composition of bio-mimetic membrane composed of 80% of Lipoid® S100 and 20% of cholesterol in n-octanol 10% w/w solution, at 37 ± 0.5°C and 14,000 rpm. The predictive capacity of the permeability of passive diffusion absorbed compounds was evaluated using 20 drug standards and showed an exponential correlation between the apparent permeability coefficients (Papp ) and the fraction absorbed percentages in humans (Fa%), with an R2 value of 0...
June 19, 2018: AAPS PharmSciTech
Mathieu Menard, Mathieu Domalain, Arnaud Decatoire, Patrick Lacouture
Knee functional disorders are one of the most common lower extremity non-traumatic injuries reported by cyclists. Incorrect bicycle configuration may predispose cyclist to injury but the evidence of an effect of saddle setback on knee pain remains inconclusive. The aim of this study was to determine the effect of saddle setback on knee joint forces during pedalling using a musculoskeletal modelling approach. Ten cyclists were assessed under three saddle setback conditions (range of changes in saddle position ~6 cm) while pedalling at a steady power output of 200 W and cadence of 90 rpm...
June 19, 2018: Sports Biomechanics
Moritz Vollrath, Julia Engert, Gerhard Winter
The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc) extruder. In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics. It is difficult to attribute release characteristics to only one parameter, since the release will always be influenced by a combination of parameters. In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run...
June 15, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Csaba Bartos, Orsolya Jójárt-Laczkovich, Gábor Katona, Mária Budai-Szűcs, Rita Ambrus, Alexandra Bocsik, Ilona Gróf, Mária Anna Deli, Piroska Szabó-Révész
Purpose: The article reports a wet milling process, where the planetary ball mill was combined with pearl milling technology to reach nanosize range of meloxicam (Mel; 100-500 nm). The main purpose was to increase the dissolution rate and extent of a poorly water-soluble Mel as nonsteroidal anti-inflammatory drug as well as to study its permeability across cultured intestinal epithelial cell layers. Methods: Viscosity of milled dispersion and particle size distribution and zeta potential of Mel were investigated and differential scanning calorimeter and X-ray powder diffractometer were used to analyse the structure of the suspended Mel...
2018: Drug Design, Development and Therapy
Alessandra Oliva, Maria Teresa Mascellino, Bich Lien Nguyen, Massimiliano De Angelis, Alessia Cipolla, Annalisa Di Berardino, Antonio Ciccaglioni, Claudio Maria Mastroianni, Vincenzo Vullo
Introduction: Sonication showed more sensitivity than traditional culture in the diagnosis of device infections. Aims of the study were to assess the role of sonication in the microbiological diagnosis and management of cardiac device infections (CDIs), to evaluate the sensitivity of sonication in patients on antimicrobial therapy at the time of device removal, and to analyze biofilm formation of the isolated strains. Materials and Methods: A total of 90 devices (31 generators and 59 electrodes) collected from 31 patients with infection underwent sonication before culture...
April 2018: Journal of Global Infectious Diseases
Ozgur Ozkaya, Gorkem Aybars Balci, Hakan As, Emre Vardarli
Although reliability correlations of traditional power indices of the Wingate test have been well documented, no study has analyzed new generation power indices based on milliseconds obtained from a Peak Bike. The purpose of this study was to investigate the retest reliability of new generation power indices. Thirty-two well-trained male athletes who were specialized in basketball, football, tennis, or track and field volunteered to take part in the study (age: 24.3 ± 2.2 years; body mass: 77 ± 8.3 kg; height: 180...
April 7, 2018: Sports
Xin Wu, Huijun He, William L Yang, Jiaping Yu, Chunping Yang
A novel bionanomaterial comprising Saccharomyces cerevisiae (S. cerevisiae) and Fe3 O4 nanoparticles encapsulated in a sodium alginate-polyvinyl alcohol (SA-PVA) matrix was synthesized for the efficient removal of atrazine from aqueous solutions. The effects of the operating parameters, nitrogen source, and glucose and Fe3+ contents on atrazine removal were investigated, and the intermediates were detected by gas chromatography-mass spectrometry (GC-MS). In addition, the synthesized Fe3 O4 particles were characterized by XRD, EDX, HR-TEM, FTIR, and hysteresis loops, and the bionanomaterial was characterized by SEM...
June 17, 2018: Applied Microbiology and Biotechnology
N Lopez-Ramirez, T Volke-Sepulveda, I Gaime-Perraud, G Saucedo-Castañeda, E Favela-Torres
A novel bench-scale stirred bioreactor for solid-state fermentation was used to determine the effect of the stirring rate on growth and enzymes production by Trichoderma harzianum PBLA. Lab-scale static tubular bioreactors were first used to assess the effect of bioreactor diameter on heat accumulation, growth, and production of cellulases and xylanases. The increased diameters (1.8-4.2 cm) led to increases in temperature up to 36 °C (at a rate of 1.08 °C/cm), which negatively affected the growth and enzyme production...
June 14, 2018: Bioresource Technology
C B Jacometo, A S Alharthi, Z Zhou, D Luchini, J J Loor
Pregnancy and early life are critical periods during which environmental factors such as nutrition can affect development. Rumen-protected methionine (Met; RPM) supplementation during the prepartum period improves not only performance but immune responses in dairy cows. We investigated the effects of enhanced maternal supply of Met via feeding RPM on whole-blood in vitro lipopolysaccharide (LPS; 0, 0.01, or 5 μg/mL of blood) challenge and targeted microRNA and mRNA abundance in calf blood polymorphonuclear leukocytes (PMNL)...
June 13, 2018: Journal of Dairy Science
Abdallah R Ismail, Samy B El-Henawy, Sherif A Younis, Mohamed A Betiha, Nour Sh El-Gendy, Mohamed S Azab, Nagwa M Sedky
THE AIM: of this study was to isolate and identify lipolytic bacteria. Perform a statistical stepwise physicochemical optimization for maximum production of extracellular lipase and its validation in a bioreactor. METHODS AND RESULTS: Several lipolytic bacteria were isolated from petroleum hydrocarbon polluted soil. The strain expressing the highest lipase activity (47 U ml-1 ) was genetically identified as Gram +ve Bacillus stratosphericus PSP8 (NCBI GenBank Accession no...
June 16, 2018: Journal of Applied Microbiology
Yu-Wen Huang, Hua Kwang Lee, Hou-Kuang Shih, Jen-Fon Jen
A dumbbell-shaped magnetic stir-bar with sublimate sorbent was prepared for the stir bar sorptive extraction (SBSE) of pesticides in an aqueous sample prior to gas chromatography-micro-electron capture detection (GC-μECD). Cyclododecane (CDD) was coated onto a magnetic stir-bar surface as a sublimate sorbent, and steel balls were placed on both ends to form a dumbbell-shaped magnetic stir-bar for SBSE. Four EDC pesticides including chlorpyrifos, ethion, bromopropylate, and λ-cyhalothrin in aqueous samples were selected as model species to examine the proposed SBSE and the following desorption...
June 6, 2018: Journal of Chromatography. A
Serap Gedikli, Gökhan Güngör, Yağmur Toptaş, Dilber Ece Sezgin, Murat Demirbilek, Nuray Yazıhan, Pınar Aytar Çelik, Emir Baki Denkbaş, Vural Bütün, Ahmet Çabuk
In the present study, culture conditions of Streptococcus equi was optimized through Box-Behnken experimental design for hyaluronic acid production. About 0.87 gL-1 of hyaluronic acid was produced under the determined conditions and optimal conditions were found as 38.42 °C, 24 hr and 250 rpm. The validity and practicability of this statistical optimization strategy were confirmed relation between predicted and experimental values. The hyaluronic acid obtained under optimal conditions was characterized...
June 14, 2018: Preparative Biochemistry & Biotechnology
A A Ayoola, O S I Fayomi, I F Usoro
In this research paper the production of biodiesel from palm kernel oil (PKO) using CaO obtained from waste turkey bones (WTB) and analytical grade calcium oxide was investigated. Treated WTB was reduced to fine particulate size of <150 µm and then calcinated at 800 °C for 3 h to increase its catalytic activity by its conversion from Calcium phosphate hydroxide (Ca10 P6 O26 H2 ) to CaO. X-ray diffraction (XRD) and X-ray fluorescent (XRF) analysis of the analytical grade CaO, uncalcined and calcined WTB were carried out to establish their elemental chemical composition...
August 2018: Data in Brief
O S I Fayomi, A A Daniyan, L E Umoru, A P I Popoola
In this work, a detail effect of nanoparticle loading and improved process parameter on the synthesis of modified Zn-TiO2 electrocodeposited nanocomposite coating was presented. The coatings were performed at constant time of 20 minute at a stirring rate of 400 rpm at temperature of 70 °C. The effect of particle loading and input current on the properties of the electrocodeposited Nanocomposite was studied. The co-deposition was carried out at a current interval between 1.0 and 1.5 A for the coating period...
June 2018: Data in Brief
Ayoola A Ayodeji, Igho E Blessing, Fayomi O Sunday
Biodiesel was produced from soybean oil using calcium oxide and cow bone as heterogeneous catalysts, through transesterification process. The soybean oil used was characterized using gas chromatography mass spectrometer (GCMS) and the cow bone catalyst produced was characterized X-ray fluorescence (XRF) spectrometer. The effects of the variation of methanol/oil mole ratio, catalyst concentration and reaction temperature on biodiesel yield during the transesterification of soybean oil were investigated. Reaction time of 3 h and stirring rate of 500 rpm were kept constant...
June 2018: Data in Brief
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