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Time laps microscopy

Himansu Sekhar Nanda, Ankur Harish Shah, Gautama Wicaksono, Oleksandr Pokholenko, Feng Gao, Ivan Djordjevic, Terry W J Steele
Bioadhesives are a current unmet clinical need for mending of blood contacting soft tissues without inducing thrombosis. Recent development of carbene precursor bioadhesives with the advantages of on-demand curing, tuneable modulus, and wet adhesion have been synthesized by grafting diazirine onto poly (amidoamine) (PAMAM-G5) dendrimers. Herein, the structure activity relationships of platelet adhesion and activation is evaluated for the first time on the cured PAMAM-g-diazirine bioadhesives. Three strategies were employed to prevent healthy human donor platelets from adhering and activating on light cured bioadhesive surfaces: 1) Attenuation of cationic surface charge, 2) anti-fouling composites by incorporating heparin and alginate in uncured formulation, and 3) heparin wash of cured bioadhesive surface...
February 9, 2018: Biomacromolecules
Weiwei Wu, Yuangang Zu, Li Wang, Lingling Wang, Huimei Wang, Yuanyuan Li, Mingfang Wu, Xiuhua Zhao, Yujie Fu
The present work aimed to apply the liquid antisolvent precipitation (LAP) method for preparing the apigenin nanoparticles and thereby improving the solubility and bioavailability of apigenin. The different experimental parameters on particle size were optimized through central composite design (CCD) using the Design-Expert(®) software. Under the optimum conditions, the particle size of the apigenin nanosuspension was about 159.2 nm. In order to get apigenin nanoparticles, the freeze-drying method was selected and the mannitol was used as a cryoprotectant...
November 2017: Drug Delivery
Fan Wu, De-Wen Zhang, Jian Wang, Michael Watkinson, Steffi Krause
A copper(I)-catalyzed azide alkyne cycloaddition (CuAAC) reaction combined with microcontact printing was used successfully to pattern alkyne-terminated self-assembled organic monolayer-modified silicon surfaces. Despite the absence of a copper peak in X-ray photoelectron spectra, copper contamination was found and visualized using light-addressable potentiometric sensors (LAPS) and scanning photo-induced impedance microscopy (SPIM) after the "click"-modified silicon surfaces were rinsed with hydrochloric acid (HCl) solution, which was frequently used to remove copper residues in the past...
March 24, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
Yuangang Zu, Weiwei Wu, Xiuhua Zhao, Yong Li, Weiguo Wang, Chen Zhong, Yin Zhang, Xue Zhao
Taxifolin is a kind of flavanonol, whose antioxidant ability is superior to that of ordinary flavonoids compounds owing to its special structure. However, its low bioavailability is a major obstacle for biomedical applications, so the experiment is designed to prepare taxifolin nanoparticles by liquid antisolvent precipitation (LAP) to improve its bioavailability. We selected ethanol as solvent, deionized water as antisolvent, and investigated primarily the type of surfactant and adding amount, drug concentration, volume ratio of antisolvent to solvent, precipitation temperature, dropping speed, stirring speed, stirring time factors affecting drug particles size...
August 25, 2014: International Journal of Pharmaceutics
André de Lima Aires, Eulália Camelo Pessoa Azevedo Ximenes, Renata Alexandre Ramos Silva, Vanessa Xavier Barbosa, Alexandre José da Silva Góes, Christina Alves Peixoto, Valdênia Maria Oliveira Souza, Mônica Camelo Pessôa de Azevedo Albuquerque
The present study provides, for the first time, conclusions on the in vitro schistosomicidal properties of β-lap. Adult male Schistosoma mansoni worms of the BH strain were used for the study. Motility, mortality, cell viability and alterations in the tegument were employed as schistosomicidal parameters. Alterations in motility were observed 6h after incubation in concentrations of 50 and 100 μM. β-lap decreased significantly the worm viability, reducing the formation of formazan in 17.7%, 27.4% and 54...
July 2014: Experimental Parasitology
Yuangang Zu, Wei Sun, Xiuhua Zhao, Weiguo Wang, Yong Li, Yunlong Ge, Ying Liu, Kunlun Wang
We prepared amphotericin B (AmB) nanoparticles through liquid antisolvent precipitation (LAP) and by freeze-drying to improve the solubility of AmB for oral administration. The LAP was optimized through a single-factor experiment. We determined the effects of surfactants and their concentration, the stirring time, the precipitation temperature, the stirring intensity, the drug concentration and the volume ratio of antisolvent to solvent on the mean particle size (MPS) of the AmB nanoparticles. Increased stirring intensity and precipitation time favored AmB nanoparticles with smaller MPS, but precipitation times exceeding 30 min did not further reduce the MPS...
March 12, 2014: European Journal of Pharmaceutical Sciences
Karen E Trainor, Aaron Roberts, Brad Weeks, Nicolette Jackson, Richard W Troughton, Christopher J Charles, Miriam T Rademaker, Iain C Melton, Ian G Crozier, Werner Hafelfinger, Dan E Gutfinger, Neal L Eigler, William T Abraham, Fred J Clubb
A left atrial pressure (LAP) monitoring system was developed for guiding the management of patients with heart failure. The LAP sensor is implanted into the left atrium via transseptal catheterization and affixed to the interatrial septum by nitinol anchors. The long-term safety of permanent implantation of the LAP sensor in patients was evaluated based on the comparative pathology in animals. Tissue specimens of the LAP sensor from 7 patients, 49 canines, and 14 ovine were examined for thrombosis and tissue overgrowth...
September 2013: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Bae Hyun Shin, Yunki Lim, Hye Jin Oh, Sang Min Park, Sun-Kyung Lee, Joohong Ahnn, Do Han Kim, Woo Keun Song, Tae Hwan Kwak, Woo Jin Park
Intracellular accumulation of polyglutamine (polyQ)-expanded Huntingtin (Htt) protein is a hallmark of Huntington's disease (HD). This study evaluated whether activation of Sirt1 by the anti-cancer agent, β-lapachone (β-lap), induces autophagy in human neuroblastoma SH-SY5Y cells, thereby reducing intracellular levels of polyQ aggregates and their concomitant cytotoxicity. Treatment of cells with β-lap markedly diminished the cytotoxicity induced by forced expression of Htt exon 1 containing a pathogenic polyQ stretch fused to green fluorescent protein (HttEx1(97Q)-GFP)...
2013: PloS One
Henrik Failmezger, Paurush Praveen, Achim Tresch, Holger Fröhlich
MOTIVATION: As RNA interference is becoming a standard method for targeted gene perturbation, computational approaches to reverse engineer parts of biological networks based on measurable effects of RNAi become increasingly relevant. The vast majority of these methods use gene expression data, but little attention has been paid so far to other data types. RESULTS: Here we present a method, which can infer gene networks from high-dimensional phenotypic perturbation effects on single cells recorded by time-lapse microscopy...
June 15, 2013: Bioinformatics
Lei Shao, Andrei S Susha, Lap Shan Cheung, Tapan K Sau, Andrey L Rogach, Jianfang Wang
Gold nanostars, possessing multiple sharp spikes, have emerged as promising plasmonic particles in the field of ultrasensitive sensing. We have developed a water-based method for high-yield synthesis of size-tunable anisotropic gold nanoparticles with a varying number of spiky surface protrusions, and performed systematic experimental and theoretical analyses of the optical properties of the single gold nanostars by characterizing them simultaneously with scanning electron microscopy and dark-field scattering spectroscopy...
June 19, 2012: Langmuir: the ACS Journal of Surfaces and Colloids
Anne Palitsch, Matthias Hannig, Paul Ferger, Markus Balkenhol
OBJECTIVES: The connection between resin denture teeth and the denture base is essential for the integrity of partial and full dentures. The aim of the present study was to analyse the bond strength of acrylic denture teeth to two light curing denture base materials compared to the gold-standard (MMA/PMMA) using different conditioning liquids. METHODS: The ridge laps of 220 identical denture teeth were ground and pre-treated using different conditioning liquids (MMA, an experimental conditioning liquid as well as the two commercially available liquids Palabond and Versyo...
March 2012: Journal of Dentistry
King-Chung Lee, Lap-Sam Mak
Ultrastructural examination is a time-consuming and tiring process, requiring search for diagnostic features on a low-contrast screen in a dim environment. This article describes a method to circumvent these problems through the creation of a virtual ultrathin slide. This can be achieved by automated capturing of hundreds of images at high magnification and stitching them together into a digital image with a resolution of 4 nm/pixel. The pathologist can then navigate the virtual slide at his/her workstation computer...
October 2011: International Journal of Surgical Pathology
Murat Kurt, Yakup Şinasi Saraç, Cağrı Ural, Duygu Saraç
OBJECTIVES: This study evaluated the effects of various pre-processing methods on the bond strength between resin and denture teeth. BACKGROUNDS: Debonding of acrylic resin teeth from denture base material is a problem for patients wearing complete dentures. MATERIALS AND METHODS: Four experimental groups (n = 30) were investigated by subjecting tooth-resin bonding to tensile loading. Specimens were prepared and tested according to the methods of the International Standards Organization (ISO 22112:2005) using a special assembly...
June 2012: Gerodontology
Hanna Ksiezak-Reding, Lap Ho, Ismael Santa-Maria, Carmen Diaz-Ruiz, Jun Wang, Giulio Maria Pasinetti
Abnormal folding of the microtubule-associated protein tau leads to aggregation of tau into paired helical filaments (PHFs) and neurofibrillary tangles, the major hallmark of Alzheimer's disease (AD). We have recently shown that grape seed polyphenol extract (GSPE) reduces tau pathology in the TMHT mouse model of tauopathy (Wang et al., 2010). In the present studies we assessed the impact of GSPE exposure on the ultrastructure of PHFs isolated from Alzheimer's disease brain. Transmission electron microscopy revealed that GSPE induced profound dose- and time-dependent alterations in the morphology of PHFs with partial disintegration of filaments...
July 2012: Neurobiology of Aging
J Oliva Encina, A Marco Valdenebro, J Pelegrí Gabarró, C Rioja Sanz
Cystoscopy is gold-standard method in non muscule invasive bladder cancer diagnosis. In the cistoscopic exploratión 30% of tumors could be overlooked: it is due to a flat forms, little size tumours or difficult visualization. Photodynamic diagnosis reduces overlooked tumours rate, and has improved diagnosis on flat forms, with the consequence of increasing lap time to recurrence and decreasing the number of iterative cistoscopy; nevertheless the false positive rate is high. In the last years developed new optical devices who try to improve alone or associated diagnostic sensibility in cistoscopy without reduction of specificity...
September 2010: Actas Urologicas Españolas
Jigang Wu, Xiquan Cui, Guoan Zheng, Ying Min Wang, Lap Man Lee, Changhuei Yang
We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm x 5 mm and acquisition time of 2.5 s. The resolution is fundamentally limited by the spot size--our demonstrated average FWHM spot diameter was 0...
July 1, 2010: Optics Letters
H X Wang, S K Guan, X Wang, C X Ren, L G Wang
The key to manufacturing magnesium-based alloys that are suitable as biodegradable orthopaedic implants is how to adjust their degradation rates and mechanical integrity in the physiological environment. In this study, to solve this challenge, a soluble Ca-deficient hydroxyapatite (Ca-def HA) coating was deposited on an Mg-Zn-Ca alloy substrate by pulse eletrodeposition. This deposition can be demonstrated by X-ray diffractometry and energy dispersion spectroscopy analyses, and the Ca/P atomic ratio of as-deposited coating is about 1...
May 2010: Acta Biomaterialia
Gudjón Andri Gylfason, Erna Knútsdóttir, Bjarni Asgeirsson
Lipid rafts are glycosphingolipid/cholesterol-enriched membrane microdomains that have been extensively studied during the past two decades. Our aim was to isolate and perform biochemical characterization of lipid rafts from the intestinal brush border membrane (BBM) of Atlantic cod (Gadus morhua) to confirm their existence in a cold-water species and compare their characteristics with lipid rafts from other species in terms of lipid and protein content. To validate the isolation process, we assayed marker enzymes for subcellular organelles, including alkaline phosphatase (AP) and leucine aminopeptidase (LAP), both well-known marker enzymes for BBM and lipid rafts...
January 2010: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Wesley B Williams, Brigid A Mullany, Wesley C Parker, Patrick J Moyer, Mark H Randles
Grinding, lapping, and polishing are finishing processes used to achieve critical surface parameters in a variety of precision optical and electronic components. As these processes remove material from the surface through mechanical and chemical interactions, they may induce a damaged layer of cracks, voids, and stressed material below the surface. This subsurface damage (SSD) can degrade the performance of a final product by creating optical aberrations due to diffraction, premature failure in oscillating components, and a reduction in the laser induced damage threshold of high energy optics...
September 20, 2009: Applied Optics
T Sultana, G L Georgiev, R J Baird, G W Auner, G Newaz, R Patwa, H J Herfurth
Biomedical devices and implants require precision joining for hermetic sealing which can be achieved with low power lasers. The effect of two different thin metal film coating methods was studied in transmission laser micro-joints of titanium-coated glass and polyimide. The coating methods were cathodic arc physical vapor deposition (CA-PVD) and electron beam evaporation (EB-PVD). Titanium-coated glass joined to polyimide film can have neural electrode application. The improvement of the joint quality will be essential for robust performance of the device...
July 2009: Journal of the Mechanical Behavior of Biomedical Materials
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