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Embryo day3

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https://www.readbyqxmd.com/read/27497663/vitrification-at-day3-stage-appears-not-to-affect-the-methylation-status-of-h19-igf2-differentially-methylated-region-of-in%C3%A2-vitro-produced-human-blastocysts
#1
Marzieh Derakhshan-Horeh, Farid Abolhassani, Farnoosh Jafarpour, Ashraf Moini, Khadijeh Karbalaie, Sayyed Morteza Hosseini, Mohammad Hossein Nasr-Esfahani
One of the most widely used assisted reproductive technology (ART) is vitrification. The aim of this study is to evaluate DNA methylation of H19/IGF2 differentially methylation region (DMR) in in vitro produced human blastocysts derived from non-vitrified and vitrified day3 embryos. Day3 embryos derived from ICSI cycles from fertile couples referring for family balancing program were either biopsied or vitrified/warmed and subsequently biopsied. Following biopsy, embryos were cultured to day 5. Day5 blastocysts with desired sex were transferred or vitrified for future use...
October 2016: Cryobiology
https://www.readbyqxmd.com/read/23274513/a-highly-sensitive-electrochemiluminescence-immunoassay-for-detecting-human-embryonic-human-chorionic-gonadotropin-in-spent-embryo-culture-media-during-ivf-et-cycle
#2
Chen Xiao-Yan, Li Jie, Jiang Dang, Li Tao, Liu Xin-Ru, Zhuang Guang-Lun
PURPOSE: To investigate the stability and repeatability of electrochemiluminescence immunoassay (ECLIA) for beta-hCG detection in embryo spent culture media. To evaluate the correlation between the viability of preimplantation embryo and beta-hCG profile by the new assay. METHODS: In a retrospective study, a total of 357 spent culture media from day1 to day5 were individually collected and quantified by ECLIA. The blank controls and reliability test were performed with normal saline/pure culture media...
March 2013: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/19015022/multi-stage-analysis-of-gene-expression-and-transcription-regulation-in-c57-b6-mouse-liver-development
#3
Tingting Li, Jian Huang, Ying Jiang, Yan Zeng, Fuchu He, Michael Q Zhang, Zeguang Han, Xuegong Zhang
The liver performs a number of essential functions for life. The development of such a complex organ relies on finely regulated gene expression profiles which change over time in the development and determine the phenotype and function of the liver. We used high-density oligonucleotide microarrays to study the gene expression and transcription regulation at 14 time points across the C57/B6 mouse liver development, which include E11.5 (embryonic day 11.5), E12.5, E13.5, E14.5, E15.5, E16.5, E17.5, E18.5, Day0 (the day of birth), Day3, Day7, Day14, Day21, and normal adult liver...
March 2009: Genomics
https://www.readbyqxmd.com/read/18648497/expression-analysis-of-the-nlrp-gene-family-suggests-a-role-in-human-preimplantation-development
#4
Pu Zhang, Morag Dixon, Marco Zucchelli, Fredwell Hambiliki, Lev Levkov, Outi Hovatta, Juha Kere
BACKGROUND: The NLRP (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing) family, also referred to as NALP family, is well known for its roles in apoptosis and inflammation. Several NLRPs have been indicated as being involved in reproduction as well. METHODOLOGY: We studied, using the unique human gametes and embryo materials, the expression of the NLRP family in human gametes and preimplantation embryos at different developmental stages, and compared the expression levels between normal and abnormal embryos using real-time PCR...
2008: PloS One
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