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https://www.readbyqxmd.com/read/28807662/a-preliminary-study-of-osmotic-dehydration-in-zebrafish-embryos-implications-for-vitrification-and-ultra-fast-laser-warming
#1
Michelle H Connolly, Estefania Paredes, Peter Mazur
To date, traditional cryopreservation techniques have not been amenable to zebrafish (Danio rerio) embryos, due in part to their large yolky eggs, which have a low surface to volume ratio and limited permeability to water and cryoprotectants. However, recent vitrification and ultra-rapid warming studies in mice have demonstrated successful preservation by dehydrating 85% or more of their total water content. We hypothesized that this approach may help overcome the barriers to embryo cryopresrvation among D...
August 11, 2017: Cryobiology
https://www.readbyqxmd.com/read/28799183/utilization-of-porcine-in%C3%A2-vitro-produced-parthenogenetic-embryos-for-co-transfer-with-vitrified-and-warmed-embryos
#2
Yoshiyuki Nakamura, Kazuhiro Kikuchi
The present study was conducted to evaluate the possibility of using in vitro-produced parthenogenetic (PA) embryos for co-transfer with morulae that had been collected in vivo and cryopreserved. The proportion of PA blastocysts (20.5%) was higher than that of their in vitro fertilization (IVF) counterparts (16.6%). Although there were no differences in morphology or diameter between the two groups, the number of cells in early PA blastocysts after in vitro culture for 6 days was lower than for IVF blastocysts (25...
August 10, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28798080/long-term-programming-effect-of-early-hypoxia-and-high-carbohydrate-diet-at-first-feeding-on-glucose-metabolism-in-rainbow-trout-juveniles
#3
Jingwei Liu, Karine Dias, Elisabeth Plagnes-Juan, Vincent Véron, Stéphane Panserat, Lucie Marandel
Environmental conditions experienced during early life play an important role in the long-term metabolic status of individuals. The present study investigated whether early hypoxia exposure (24 h, 2.5 mg·l(-1), 20% dissolved O2) during embryo stage alone (hypoxic history) or combined with a 5-day high carbohydrate diet (60%) stimulus at first-feeding (high carbohydrate diet history) can affect the glucose metabolism later in life, i.e. in fish juveniles. After 19 weeks of growth, we observed a decrease in final body weight in fish with a high carbohydrate diet history...
August 10, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28797287/is-a-high-serum-copper-concentration-a-risk-factor-for-implantation-failure
#4
Hidehiko Matsubayashi, Kotaro Kitaya, Kohei Yamaguchi, Rie Nishiyama, Yukiko Takaya, Tomomoto Ishikawa
BACKGROUND: Copper-containing contraceptive devices may deposit copper ions in the endometrium, resulting in implantation failure. The deposition of copper ions in many organs has been reported in patients with untreated Wilson's disease. Since these patients sometimes exhibit subfertility and/or early pregnancy loss, copper ions were also considered to accumulate in the uterine endometrium. Wilson's disease patients treated with zinc successfully delivered babies because zinc interfered with the absorption of copper from the gastrointestinal tract...
August 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28796907/likelihood-of-pregnancy-after-embryo-transfer-is-reduced-in-recipient-mares-with-a-short-preceding-oestrus
#5
J Cuervo-Arango, A N Claes, M Ruijter-Villani, T A Stout
BACKGROUND: Previous surveys reported a positive association between the length of the follicular phase and subsequent fertility in embryo transfer donor and Thoroughbred mares. However, it is unclear whether a longer oestrus positively influences fertilisation and oviductal development (oocyte quality, oviductal environment), or uterine receptivity and survival of the embryo in the uterus. OBJECTIVES: To determine the effect of length of oestrus (characterised by duration of endometrial oedema) on likelihood of pregnancy and early embryo loss (EEL) in recipient mares after embryo transfer (ET)...
August 10, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28796038/clomiphene-citrate-is-associated-with-favorable-cycle-characteristics-but-impaired-outcomes-of-obese-women-with-polycystic-ovarian-syndrome-undergoing-ovarian-stimulation-for-in-vitro-fertilization
#6
Shutian Jiang, Yanping Kuang
The aim of this study was to explore the effect of clomiphene citrate (CC) on the cycle characteristics and outcomes of obese women with polycystic ovarian syndrome (PCOS) undergoing ovarian stimulation for in vitro fertilization (IVF).This is a retrospective cohort study, and it was conducted at the tertiary-care academic medical center.This study included 174 obese PCOS patients undergoing IVF.In the study group (n = 90), CC and human menopausal gonadotropin (HMG) were administered simultaneously beginning on cycle day 3, while in control group (n = 84) HMG was used only...
August 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28791838/collagenous-scaffolds-supplemented-with-hyaluronic-acid-and-chondroitin-sulfate-used-for-wound-fibroblast-and-embryonic-nerve-cell-culture
#7
Jacek Drobnik, Krystyna Pietrucha, Lucyna Piera, Jacek Szymański, Alicja Szczepanowska
BACKGROUND: Tissue engineering is a strategy aimed at improving the regeneration of injured tissues. OBJECTIVES: The aim of the present study was to determine whether a tri-copolymer composed of crosslinked collagen, chondroitin sulfate and hyaluronic acid (Col + CS + HA) provides a better environment for fibroblast and embryonic nerve cell culture than a collagenous scaffold (Col). MATERIAL AND METHODS: The porosity of each of the matrices was characterized with a scanning electron microscope...
March 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/28776828/the-quality-after-culture-in%C3%A2-vitro-or-in%C3%A2-vivo-of-porcine-oocytes-matured-and-fertilized-in%C3%A2-vitro-and-their-ability-to-develop-to-term
#8
Yoshiyuki Nakamura, Sigeyuki Tajima, Kazuhiro Kikuchi
The quality of porcine blastocysts produced in vitro is poor in comparison with those that develop in vivo. We examined the quality of in vitro-matured and fertilized (IVM/IVF) oocytes, their abilities to develop to blastocysts under in vivo and in vitro conditions, and the potential of the embryos to develop to term after transfer. IVM/IVF oocytes were either transferred and the embryos recovered on Days 5 and 6 (100% and 87.5%, respectively) ('ET-vivo' embryos), or cultured in vitro for 5 or 6 days ('IVC' embryos)...
August 4, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28774739/are-there-any-differences-between-antagonist-administration-on-days-6-and-%C3%A2-6-of-coh-on-assisted-reproductive-technique-outcomes
#9
Zeynep Ozturk Inal, Nafiye Yilmaz, Hasan Ali Inal, Necati Hancerliogullari, Bugra Coskun
BACKGROUND: The aim of this study was to investigate the cost-effectiveness of antagonist administration on stimulation on days <6 and ≥6 of COH on assisted reproductive technique (ART) outcomes. METHODS: In this retrospective cohort study, 412 patients who were admitted to the ART Department were evaluated. In group 1 (203 patients), antagonist administration was provided on days <6 of COH. For group 2 (209 patients), antagonist administration was provided on days ≥6 of COH...
July 31, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28772113/structural-integrity-and-developmental-potential-of-spermatozoa-following-microwave-assisted-drying-in-the-domestic-cat-model
#10
Jennifer L Patrick, Gloria D Elliott, Pierre Comizzoli
Characterizing the resilience of mammalian cells to non-physiological conditions is necessary to develop preservation and long-term storage strategies at low or ambient temperatures. Using the domestic cat model, the objective of the study was to characterize structural integrity (morphology and DNA damage) as well as functional properties (sperm aster formation and embryo formation after sperm injection) of spermatozoa after microwave-assisted drying to a moisture content compatible with storage in a glassy state at supra-zero temperatures...
July 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28770245/molecular-characterization-and-phylogenetic-analyses-of-virulent-infectious-bronchitis-viruses-isolated-from-chickens-in-eastern-saudi-arabia
#11
Maged Gomaa Hemida, Mohammed A Al-Hammadi, Abdul Hafeed S Daleb, Cecilio R Gonsalves
Infectious bronchitis virus (IBV) is one of the major respiratory viral threats for chickens. Despite the intensive application of IBV vaccines, several outbreaks have been reported worldwide. Here, we report several IBV outbreaks in thirteen poultry farms in Eastern Saudi Arabia (ESA) from 2013 to 2014. The main goals of the current study were as follows: (1) isolation and molecular characterization of the currently circulating strains in ESA (Al-Hasa, Dammam, and Buqayq) and (2) evaluation of the immune status of these birds to IBV...
June 2017: Virusdisease
https://www.readbyqxmd.com/read/28767159/effects-of-in-ovo-exposure-to-3-3-4-4-tetrachlorobiphenyl-pcb-77-on-heart-development-in-tree-swallow-tachycineta-bicolor
#12
Tiffany Carro, Mary K Walker, Karen M Dean, Mary Ann Ottinger
Tree swallow (Tachycineta bicolor) eggs from two uncontaminated sites, Patuxent Research Refuge (PRR), MD and Cobleskill Reservoir, NY were dosed with PCB 77 to evaluate effects on the developing cardiovascular system. To ensure embryonic viability, treatments were administered into the air cell at embryonic day (ED) 2.5 including: untreated (control), vehicle (filtered sterilized fatty acid mixture), 100 ng/g and 1,000 ng/g egg. Eggs were dosed in the field with 0.2 μl per egg, returned to the nest, collected at ED13, hatched in the laboratory and necropsied...
August 2, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28763726/effect-of-different-hormonal-combinations-on-follicular-wave-emergence-and-superovulatory-response-in-sheep
#13
Joanna Maria Gonçalves Souza-Fabjan, Rômulo Mendonça da Rosa, Mário Felipe Alvarez Balaro, Pedro Henrique Nicolau Pinto, Gustavo Bervian Dos Santos, Eduardo Kenji Nunes Arashiro, Jeferson Ferreira da Fonseca, Rodolfo Ungerfeld, Felipe Zandonadi Brandão
The aim of the present study was to compare hormonal treatments to induce and synchronize follicular wave emergence to improve the results of superovulatory (SOV) treatments in ewes. In Experiment 1 (n = 66), ewes were treated with a progesterone intravaginal implant plus a PGF2α analogue (group GP4), or with the same treatment plus estradiol benzoate (GP4+EB), a GnRH agonist (GP4+GnRH), or both, estradiol benzoate and a GnRH agonist (GP4+EB+GnRH) in a 2 × 2 factorial arrangement. Follicular wave emergence was determined by ultrasound...
July 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/28758253/mir-182-selectively-targets-hoxa10-in-goat-endometrial-epithelium-cells-in-vitro
#14
L Zhang, X-R Liu, J-Z Liu, Y-X Song, Z-Q Zhou, B-Y Cao
Proper HOXA10 expression was essential for endometrial receptivity what was crucial for successful embryo implantation in mammalian. This study confirmed that miR-182 regulated the expression levels of HOXA10 by binding to its 3' UTR, selectively downregulated HOXA10 in goat endometrial epithelium cells (gEECs) but not stromal cell (gESCs) in vitro. However, HOXA10 and miR-182 both up-expressed in the goat endometrium at gestational day 15 (D15) compared with gestational day 5 (D5), suggesting that there were some other factors regulated the expression of HOXA10 during the development of goat endometrium in vivo...
July 30, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28756497/natural-selection-between-day-3-and-day-5-6-pgd-embryos-in-couples-with-reciprocal-or-robertsonian-translocations
#15
Claire E Beyer, E Willats
PURPOSE: For translocation carriers, preimplantation genetic diagnosis (PGD) provides the opportunity to distinguish between normal/balanced and unbalanced embryos prior to implantation and, as such, increases the likelihood of a successful ongoing pregnancy. The data presented here compares autosomal reciprocal and Robertsonian translocation segregation patterns in day 3 versus day 5/6 IVF-PGD embryos to determine if there is a difference in the chromosome segregation patterns observed at these developmental time points...
July 29, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28755343/histological-analysis-of-the-developmental-stages-of-direct-somatic-embryogenesis-induced-from-in-vitro-leaf-explants-of-date-palm
#16
Ola H Abdelbar
Somatic embryogenesis is an ideal technique for the micropropagation of date palm using different explant tissue; however, histological studies describing the ontogenesis of plant regeneration are limited. This chapter provides a simple protocol for the histological analysis of the successive developmental stages of direct somatic embryogenesis induced from in vitro leaf explants. Direct somatic embryos are obtained from Murashige and Skoog (MS) medium containing 2 mg/L 6-benzylaminopurine. In order to observe the different developmental stages, histological analysis is carried out on samples at 15-day intervals for 60 days...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28752967/-expression-of-hsp70-in-kunming-mouse-embryos-stimulated-by-chronic-mild-anticipatory-stress
#17
Xiao-Hong Li, Shan Luo, Wei Fan, Xun Zeng, Yu Bai, Lang Qin, Shang-Wei Li
OBJECTIVE: To measure the expression of heat shock protein 70 (HSP70) in mice embryos with the stimulation of chronic mild anticipatory stress (CUMS) to female Kunming mice. METHODS: Three hundreds female Kunming mice were stressed by 9 chronic mild unpredictable stress factors for 28 days and then divided into three groups of mild, moderate and severe stress. PMSG/hCG was measured to assess the induction of superovulation, and ovarian response and embryo development potential were observed...
July 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28744794/propensity-score-matched-study-and-meta-analysis-of-cumulative-outcomes-of-day-2-3-versus-day-5-6-embryo-transfers
#18
Ye Yin, Ge Chen, Kezhen Li, Qiuyue Liao, Sijia Zhang, Nieying Ma, Jing Chen, Yan Zhang, Jihui Ai
The superiority of the cumulative outcomes of day 5/6 embryo transfer to those of day 2/3 embryo transfer in infertile couples has been debated. This retrospective study included data collected from 1051 patients from July 2011 to June 2014. Multiple maternal baseline covariates were subjected to propensity score matching analysis, and each day 5/6 group woman was matched to one day 2/3 group woman. A systematic meta-analysis was conducted to validate the results. After matching was completed, 217 patients on the day 2/3 group were matched with those on the day 5/6 group, and no significant differences in the baseline characteristics were observed between the two groups...
July 25, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28737450/initial-characterization-of-the-growth-stimulation-and-heat-shock-induced-adaptive-response-in-developing-lake-whitefish-embryos-after-ionizing-radiation-exposure
#19
Christopher Thome, Charles Mitz, Emily N Hulley, Christopher M Somers, Richard G Manzon, Joanna Y Wilson, Douglas R Boreham
Ionizing radiation is known to effect development during early life stages. Lake whitefish (Coregonus clupeaformis) represent a unique model organism for examining such effects. The purpose of this study was to examine how ionizing radiation affects development in lake whitefish embryos and to investigate the presence of an adaptive response induced by heat shock. Acute exposure to (137)Cs gamma rays was administered at five time points corresponding to major developmental stages, with doses ranging from 0...
July 24, 2017: Radiation Research
https://www.readbyqxmd.com/read/28736370/-pregnancy-and-obstetric-outcomes-of-fresh-embryo-transfer-versus-frozen-thawed-embryo-transfer-in-women-below-35-years-of-age
#20
Ling Sun, Zhi-Heng Chen, Min-Na Yin, Yu Deng
OBJECTIVE: To compare the obstetric and perinatal outcomes between fresh embryo transfer (ET) and frozen-thawed ET (the "freeze-all" strategy) and evaluate the benefits of the "freeze-all" embryo strategy for young patients. METHODS: We reviewed a total of 2091 ET cycles performed between January, 2011 and December, 2015 in women aged 20-35 years, including 1295 fresh ET cycles and 796 frozen-thawed ET cycles. The demographic characteristics, ovarian stimulation syndrome, clinical pregnancy rates, live birth rate and the obstetric outcomes (gestational age, preterm delivery rate and mean birth weight) were compared between the two groups...
July 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
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