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https://www.readbyqxmd.com/read/29334275/laparoscopic-ovarian-drilling-versus-gnrh-antagonist-combined-with-cabergoline-as-a-prophylaxis-against-the-re-development-of-ovarian-hyperstimulation-syndrome
#1
Emaduldin Seyam, Enas Hefzy
OBJECTIVE: The aim of this work was to investigate the value of laparoscopic ovarian drilling (LOD) compared with GnRH antagonist flexible protocol combined with cabergoline (Cb), as a prophylaxis against the re-development of ovarian hyperstimulation syndrome (OHSS) in women with clomiphene citrate-resistant polycystic ovary disease (CCR-PCOD) who had severe OHSS before in a previous ICSI cycle. STUDY DESIGN: It is a prospective controlled study, where 250 CCR-PCOD women (n = 250) with a history of severe OHSS before, had been recruited for the study...
January 15, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29332610/improvements-in-the-conception-rate-milk-composition-and-embryo-quality-of-rabbit-does-after-dietary-enrichment-with-n-3-polyunsaturated-fatty-acids
#2
M Rodríguez, R M García-García, M Arias-Álvarez, P Millán, N Febrel, N Formoso-Rafferty, J López-Tello, P L Lorenzo, P G Rebollar
This work attempts to confirm the effect of an enriched diet with n-3 polyunsaturated fatty acids (PUFA) trying to mitigate the reproductive performances issues such as low conception rate of primiparous rabbits. A total of 127 does were fed ad libitum throughout their two first cycles with two diets with different fat sources: mixed fat in the control and salmon oil in the enriched one, with 3.19 g/100 g (n=63 does) and 28.77 g/100 g (n=64 does) of n-3 of the total fatty acid, respectively. Feed intake was similar between groups (P>0...
January 15, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/29331829/additional-small-dose-of-prostaglandin-f2%C3%AE-at-timed-artificial-insemination-failed-to-improve-pregnancy-risk-of-lactating-dairy-cows
#3
J A Sauls, B E Voelz, L G D Mendonça, J S Stevenson
Two experiments were performed to test the hypothesis that administering PGF2α concurrent with timed artificial insemination (AI) in lactating dairy cows would enhance pregnancy per AI (P/AI). In experiment 1, lactating Holstein cows (n = 289) in one herd were enrolled after a non-pregnancy diagnosis (30-36 d after AI) to synchronize subsequent ovulation before AI. Cows were assigned randomly to receive (im) 10 mg of PGF2α concurrent with timed AI (Day 0; treatment) or no injection (control). Blood samples were collected on Days -3, 0, and 13 to determine serum concentrations of progesterone...
January 3, 2018: Theriogenology
https://www.readbyqxmd.com/read/29331235/antim%C3%A3-llerian-hormone-as-a-predictor-of-live-birth-following-assisted-reproduction-an-analysis-of-85-062-fresh-and-thawed-cycles-from-the-society-for-assisted-reproductive-technology-clinic-outcome-reporting-system-database-for-2012-2013
#4
Reshef Tal, David B Seifer, Ethan Wantman, Valerie Baker, Oded Tal
OBJECTIVE: To determine if serum antimüllerian hormone (AMH) is associated with and/or predictive of live birth assisted reproductive technology (ART) outcomes. DESIGN: Retrospective analysis of Society for Assisted Reproductive Technology Clinic Outcome Reporting System database from 2012 to 2013. SETTING: Not applicable. PATIENT(S): A total of 69,336 (81.8%) fresh and 15,458 (18.2%) frozen embryo transfer (FET) cycles with AMH values...
January 11, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29331034/effects-of-in-situ-selenium-exposure-and-maternal-transfer-on-survival-and-deformities-of-brown-trout-salmo-trutta-fry
#5
Sean M Covington, Rami B Naddy, Alan L Prouty, Steven A Werner, Mark Dunn Lewis
Offspring of wild adult brown trout exposed to a range of selenium (Se) concentrations were reared in a laboratory setting to primarily assess effects on survival and deformities. Maternal whole body Se concentrations ranged from 4.7 to 22.6 mg/kg dry weight (dw) for wild fish. Corresponding Se concentrations in embryos ranged from 6.2 to 40.3 mg/kg dw. Significant relationships were found between embryo and whole-body tissue concentrations. Increasing egg Se concentrations were correlated with decreasing survival; however, hatch success was not significantly correlated with increasing embryo Se...
January 13, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29330027/selective-estrogen-receptor-modulator-ormeloxifene-suppresses-embryo-implantation-via-inducing-mir-140-and-targeting-insulin-like-growth-factor-1-receptor-in-rat-uterus
#6
Vijay K Sirohi, Kanchan Gupta, Rohit Kumar, Vinay Shukla, Anila Dwivedi
Ormeloxifene, the non-steroidal SERM contraceptive, inhibits endometrial receptivity and embryo implantation via countering nidatory estrogen. However, the molecular mechanism of ormeloxifene action responsible for its contraceptive efficacy still remains unclear. Herein, we aimed to identify the miRNAs modulated under the influence of ormeloxifene and to explore their role in endometrial receptivity and embryo implantation. By doing microRNA sequencing analysis, a total of 168 miRNAs were found to be differentially expressed in uterine tissue of ormeloxifene-treated rats, on day 5 (10:00 h) of pregnancy i...
January 9, 2018: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29323231/mice-deficient-in-the-shmt2-gene-have-mitochondrial-respiration-defects-and-are-embryonic-lethal
#7
Haruna Tani, Sakiko Ohnishi, Hiroshi Shitara, Takayuki Mito, Midori Yamaguchi, Hiromichi Yonekawa, Osamu Hashizume, Kaori Ishikawa, Kazuto Nakada, Jun-Ichi Hayashi
Accumulation of somatic mutations in mitochondrial DNA (mtDNA) has been proposed to be responsible for human aging and age-associated mitochondrial respiration defects. However, our previous findings suggested an alternative hypothesis of human aging-that epigenetic changes but not mutations regulate age-associated mitochondrial respiration defects, and that epigenetic downregulation of nuclear-coded genes responsible for mitochondrial translation [e.g., glycine C-acetyltransferase (GCAT), serine hydroxymethyltransferase 2 (SHMT2)] is related to age-associated respiration defects...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29323161/lack-of-fgf18-causes-abnormal-clustering-of-motor-nerve-terminals-at-the-neuromuscular-junction-with-reduced-acetylcholine-receptor-clusters
#8
Kenyu Ito, Bisei Ohkawara, Hideki Yagi, Hiroaki Nakashima, Mikito Tsushima, Kyotaro Ota, Hiroyuki Konishi, Akio Masuda, Shiro Imagama, Hiroshi Kiyama, Naoki Ishiguro, Kinji Ohno
FGF receptor 2 is involved in the formation of the neuromuscular junction (NMJ), but its in vivo ligand remains to be determined. Laser capture microdissection of the mouse spinal motor neurons (SMNs) revealed that Fgf18 mRNA is highly expressed in SMNs in adults. Expression of Fgf18 mRNA was the highest in the spinal cord at embryonic day (E) 15.5, which gradually decreased to postnatal day 7. FGF18 protein was localized at the NMJs of the tibialis anterior muscle at E18.5 and in adults. Fgf18-/- mice at E18...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320655/ivf-transfer-of-fresh-or-frozen-embryos-in-women-without-polycystic-ovaries
#9
Lan N Vuong, Vinh Q Dang, Tuong M Ho, Bao G Huynh, Duc T Ha, Toan D Pham, Linh K Nguyen, Robert J Norman, Ben W Mol
BACKGROUND: Among women who are undergoing in vitro fertilization (IVF), the transfer of frozen embryos has been shown to result in a higher rate of live birth than the transfer of fresh embryos in those with infertility associated with the polycystic ovary syndrome. It is not known whether frozen-embryo transfer results in similar benefit in women with infertility that is not associated with the polycystic ovary syndrome. METHODS: We randomly assigned 782 infertile women without the polycystic ovary syndrome who were undergoing a first or second IVF cycle to receive either a frozen embryo or a fresh embryo on day 3...
January 11, 2018: New England Journal of Medicine
https://www.readbyqxmd.com/read/29318868/embryo-development-until-blastocyst-stage-with-and-without-renewal-of-single-medium-on-day-3
#10
Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales
OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3)...
January 10, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29315421/development-of-a-new-comprehensive-and-reliable-endometrial-receptivity-map-er-map-er-grade-based-on-rt-qpcr-gene-expression-analysis
#11
M Enciso, J P Carrascosa, J Sarasa, P A Martínez-Ortiz, S Munné, J A Horcajadas, J Aizpurua
STUDY QUESTION: Is it possible to determine the receptivity status of an endometrium by combined quantitative reverse transcription PCR (RT-qPCR) expression analysis of genes involved in endometrial proliferation and immunity? SUMMARY ANSWER: The new ER Map®/ER Grade® test can predict endometrial receptivity status by RT-qPCR using a new panel of genes involved in endometrial proliferation and the maternal immune response associated to embryonic implantation. WHAT IS KNOWN ALREADY: The human endometrium reaches a receptive status adequate for embryonic implantation around Days 19-21 of the menstrual cycle...
January 5, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29313599/neurodegeneration-in-zebrafish-embryos-and-adults-after-cadmium-exposure
#12
Antonio Monaco, Teresa Capriello, Maria C Grimaldi, Valentina Schiano, Ida Ferrandino
Cadmium is a biologically non-essential metal. It is also toxic to many organs including the brain. The aim of this study was to analyse the neurodegenerative effects of this metal in embryos and adults of zebrafish exposed to sub-lethal concentrations of cadmium. The study was performed by cytochemical stainings. Six hours after fertilisation (hpf) zebrafish embryos were treated for 24 hours with 9 μM of cadmium and subsequently stained with Acridine orange in whole mount to detect apoptosis in the brain...
October 19, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29307115/bioinformatic-analyses-of-zona-pellucida-genes-in-vertebrates-and-their-expression-in-nile-tilapia
#13
Tianli Wu, Yunying Cheng, Zhilong Liu, Wenjing Tao, Shuqing Zheng, Deshou Wang
Zona pellucida (ZP) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4 ZP genes belonging to 5 subfamilies (ZPA, ZPB, ZPC, ZPD, and ZPAX) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively...
January 6, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29306198/a-glyphosate-based-herbicide-reduces-fertility-embryonic-upper-thermal-tolerance-and-alters-embryonic-diapause-of-the-threatened-annual-fish-austrolebias-nigrofasciatus
#14
Yuri Dornelles Zebral, Luize Real Lansini, Patrícia Gomes Costa, Mauricio Roza, Adalto Bianchini, Ricardo Berteaux Robaldo
Roundup is the most popular glyphosate-based herbicide (GBH) worldwide. These formulations kill a wide range of plants. Despite that, non-target species can be jeopardized by GBH, such as the annual fish Austrolebias nigrofasciatus. This species occurs in wetlands that dries annually. Key-adaptations permit them to live in such harsh habitats, e. i. Elevated fertility, drought-tolerant diapausing embryos and elevated thermal tolerance. We aimed to evaluate acute (96 h) effects of Roundup exposure (0.36 or 3...
January 2, 2018: Chemosphere
https://www.readbyqxmd.com/read/29305844/progesterone-rise-on-hcg-day-is-negatively-correlated-with-ivf-et-outcomes-in-natural-cycles
#15
Xiadi Wu, Yundong Mao, Yan Gao, Xiaoqiao Qian, Wei Wang, Wei Ding, Shi-Wen Jiang, Jiayin Liu
OBJECTIVE: To investigate the effect of progesterone rise on hCG day on the laboratory and clinical outcomes in natural cycles and to explore the possible factors related to the occurrence of progesterone elevation. PATIENTS AND STUDY METHODS: Retrospective analysis was performed in 1157 infertile women with decreased ovarian reserve. Eligible infertile women undergoing IVF in natural cycles were assigned to four groups according to serum progesterone levels on the day of hCG administration: group 1, P<2nmol/L; group 2, 2<P<2...
January 3, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29303236/andro-ivf-a-novel-protocol-for-poor-responders-to-ivf-controlled-ovarian-stimulation
#16
Ludmila Bercaire, Sara Mb Nogueira, Priscila Cm Lima, Vanessa R Alves, Nilka Donadio, Artur Dzik, Mario Cavagna, Renato Fanchin
OBJECTIVE: This study aimed to assess a novel protocol designed to improve poor ovarian response through intra-ovarian androgenization. The endpoints were: number of oocytes and mature oocytes retrieved, fertilization, cancellation and pregnancy rates. METHODS: This prospective crossover study enrolled poor responders from previous ovarian stimulation cycles submitted to a novel protocol called ANDRO-IVF. The protocol included pretreatment with transdermal AndroGel(r) (Besins) 25 mg, oral letrozole 2...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29301505/optimising-the-chick-chorioallantoic-membrane-xenograft-model-of-neuroblastoma-for-drug-delivery
#17
Rasha Swadi, Grace Mather, Barry L Pizer, Paul D Losty, Violaine See, Diana Moss
BACKGROUND: Neuroblastoma is a paediatric cancer that despite multimodal therapy still has a poor outcome for many patients with high risk tumours. Retinoic acid (RA) promotes differentiation of some neuroblastoma tumours and cell lines, and is successfully used clinically, supporting the view that differentiation therapy is a promising strategy for treatment of neuroblastoma. To improve treatment of a wider range of tumour types, development and testing of novel differentiation agents is essential...
January 4, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29289536/expression-of-pik3ip1-in-the-murine-uterus-during-early-pregnancy
#18
Hanna E Teasley, Hye Jin Chang, Tae Hoon Kim, Bon Jeong Ku, Jae-Wook Jeong
The ovarian steroid hormones, estrogen (E2) and progesterone (P4), are essential regulators of uterine functions necessary for development, embryo implantation, and normal pregnancy. ARID1A plays an important role in steroid hormone signaling in endometrial function and pregnancy. In previous studies, using high density DNA microarray analysis, we identified phosphatidylinositol-3-kinase interacting protein 1 (Pik3ip1) as one of the genes up-regulated by ARID1A. In the present study, we performed real-time RT-PCR and immunohistochemistry analysis to investigate the regulation of PIK3IP1 by ARID1A and determine expression patterns of PIK3IP1 in the uterus during early pregnancy...
December 28, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29286475/microstructured-devices-for-optimized-microinjection-and-imaging-of-zebrafish-larvae
#19
Felix Ellett, Daniel Irimia
Zebrafish have emerged as a powerful model of various human diseases and a useful tool for an increasing range of experimental studies, spanning fundamental developmental biology through to large-scale genetic and chemical screens. However, many experiments, especially those related to infection and xenograft models, rely on microinjection and imaging of embryos and larvae, which are laborious techniques that require skill and expertise. To improve the precision and throughput of current microinjection techniques, we developed a series of microstructured devices to orient and stabilize zebrafish embryos at 2 days post fertilization (dpf) in ventral, dorsal, or lateral orientation prior to the procedure...
December 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284207/production-of-transgenic-pigs-using-the-pgfap-creert2-egfploxp-inducible-system-for-central-nervous-system-disease-models
#20
Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun
Transgenic (TG) pigs are an important model for biomedical research, including disease modeling, pharmaceutical toxicity testing, and regenerative medicine. Here, we constructed 2 vector systems using the promoter of the pig glial fibrillary acidic protein (pGFAP) gene, which is an astrocyte cell marker. We established donor TG fibroblasts with pGFAP-CreERT2; LCMV-EGFPLoxP. We evaluated the effect of the transgenes on TG-SCNT embryo development. The cleavage rates were not significantly different among control and transgene-donor group...
December 28, 2017: Journal of Veterinary Science
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