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https://www.readbyqxmd.com/read/28443593/value-of-post-transfer-day-12-beta-human-chorionic-gonadotropin-levels-for-pregnancy-outcome-prediction-of-intracytoplasmic-sperm-injection-cycles
#1
İnci Kahyaoğlu, Berfu Demir, Sezin Ertürk Aksakal, İskender Kaplanoğlu, Leyla Mollamahmutoğlu
BACKGROUND: Several markers were studied previously in order to predict the pregnancy outcome of assisted reproductive techniques, however serum beta human chorionic gonadotropin (β-hCG) was found to be the most predictive marker. AIMS: The primary aim of this study was to evaluate the value of serum β-hCG levels in discriminating biochemical and clinical pregnancies 12 days after embryo transfer (ET) and to determine the factors predicting of ongoing pregnancy was established as the secondary aim...
April 13, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28442051/cerium-oxide-nanoparticles-ceo2-nps-improve-the-developmental-competence-of-in-vitro-matured-prepubertal-ovine-oocytes
#2
F Ariu, L Bogliolo, A Pinna, L Malfatti, P Innocenzi, L Falchi, D Bebbere, S Ledda
The present study investigated whether supplementation with different doses of cerium dioxide nanoparticles (CeO2 NPs) during in vitro maturation (IVM) of prepubertal ovine oocytes influenced their embryonic development in vitro. Cumulus-oocyte complexes derived from the ovaries of slaughtered prepubertal sheep underwent IVM with CeO2NPs (0, 44, 88 or 220µg mL(-1)). Matured oocytes were fertilised in vitro and zygotes were cultured for 7 days. The results demonstrated that CeO2NPs were internalised in the cumulus cells and not in the oocyte...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442049/prion-protein-2-dublet-gene-prnd-role-in-ovine-semen-capacitation-cryopreservation-and-fertility
#3
L M Ferreira, M Garcia-Herreros, A Domingos, C C Marques, P Mesquita, J P Barbas, M C Baptista, J Pimenta, A E M Horta, J A M Prates, R M L N Pereira
The aim of the present study was to examine the role of Doppel protein in the capacitation process and fertilising ability of both fresh and frozen-thawed (FT) spermatozoa from rams carrying different prion protein 2 (dublet) (PRND) gene polymorphisms. The detection efficacy of new anti-Doppel monoclonal antibodies and PRND mRNA quantification were also explored in ovine spermatozoa. Three different genotypes (AA, GA, GG) were identified for codon 26 of ovine PRND-c.78G>A. Using flow cytometry, a higher fluorescence was detected in fresh compared with FT sperm samples incubated with anti-Doppel primary and fluorescein isothiocyanate-conjugated secondary antibodies (P<0...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442048/expression-of-17%C3%AE-hydroxysteroid-dehydrogenase-and-the-effects-of-lh-fsh-and-prolactin-on-oestrone-and-17%C3%AE-oestradiol-secretion-in-the-endometrium-of-pigs-during-early-pregnancy-and-the-oestrous-cycle
#4
B Wojciechowicz, G Kotwica, K Zglejc, E Waszkiewicz, A Franczak
The endometrium of pregnant and cyclic pigs is a source of oestrone (E1) and 17β-oestradiol (E2). However, the roles of LH, FSH and prolactin (PRL) as regulators of endometrial steroidogenesis, and the presence of 17β-hydroxysteroid dehydrogenase (17β-HSD) in the porcine endometrium, remain unknown. Therefore, in the present study we examined 17β-HSD expression and the effects of LH, FSH and PRL on E1 and E2 release in vitro in endometrial explants harvested from gravid pigs on Days 10-11 (embryo migration within the uterus), 12-13 (maternal recognition of pregnancy) and 15-16 (beginning of implantation) and compared them with results obtained in non-gravid pigs...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28440715/gnrh-agonist-triggering-for-final-oocyte-maturation-in-gnrh-antagonist-ivf-cycles-induces-decreased-lh-pulse-rate-and-amplitude-in-early-luteal-phase-a-possible-luteolysis-mechanism
#5
Samer Tannus, Yechiel Burke, Christopher R McCartney, Shahar Kol
The use of GnRH agonist to trigger final oocyte maturation in GnRH-antagonist in vitro fertilization (IVF) cycles has been shown to significantly reduce or even eliminate the risk of ovarian hyperstimulation syndrome (OHSS) by inducing rapid luteolysis early in the luteal phase. The exact mechanism of this early luteolysis is still widely unknown. Since luteinizing hormone (LH) has a major role in corpus luteum support, we sought to explore the pattern of LH secretion early in the luteal phase. Ten high risk patients for developing OHSS and triggered with GnRH agonist were included...
April 25, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28440686/severe-ovarian-hyperstimulation-syndrome-after-combined-gnrh-agonist-and-low-dose-human-chorionic-gonadotropin-trigger-in-a-patient-with-a-single-kidney
#6
Nigel Pereira, Jovana P Lekovich, Isaac Kligman, Zev Rosenwaks
Ovarian hyperstimulation syndrome (OHSS) following gonadotropin-releasing hormone agonist (GnRH-a) trigger is rare. Here, we report a case of severe OHSS after combined GnRH-a and low-dose human chorionic gonadotropin (hCG) trigger in a patient with a single kidney. The patient is a 32-year-old women with a two-year history of infertility. The patient's history was significant for a single kidney, that is, she had donated a kidney to a family member three years ago. The patient underwent controlled ovarian stimulation (COS) for in vitro fertilization (IVF) and received a combined 2 mg GnRH-a and 1500 IU hCG ovulatory trigger...
April 25, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28437630/modified-natural-cycle-for-embryo-transfer-using-frozen-thawed-blastocysts-a-satisfactory-option
#7
Quoc V Le, Sina Abhari, Omar M Abuzeid, Jennifer DeAnna, Mohamed A Satti, Tarek Abozaid, Iqbal Khan, Mostafa I Abuzeid
OBJECTIVE: To describe pregnancy outcomes of frozen-thawed blastocysts cycles using modified natural cycle frozen embryo transfers (NC-FET) and down-regulated hormonally controlled frozen embryo transfers (HC-FET) protocols. STUDY DESIGN: This retrospective cohort study included all patients undergoing either modified NC-FET or down-regulated HC-FET using frozen-thawed day 5 embryos. Cycles with donor blastocysts were excluded. Four hundred twenty eight patients underwent a total of 493 FET cycles...
April 6, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28435194/molecular-characterization-of-velogenic-viscerotropic-ranikhet-newcastle-disease-virus-from-different-outbreaks-in-desi-chickens
#8
V S Dhaygude, G K Sawale, M M Chawak, N R Bulbule, S D Moregaonkar, D S Gavhane
AIM: Diagnosis of velogenic viscerotropic Ranikhet disease from six different flocks of desi chicken in and around Mumbai by gross and histopathological examination, isolation of virus and molecular methods. MATERIALS AND METHODS: A total of 25 carcasses (varying between 2 and 6 carcasses from each flock) of six different flocks of adult desi chicken were subjected to necropsy examination for diagnosis of the disease during the span of a year (2014-2015). After thorough gross examination, the tissue samples were collected and processed for virus isolation and histopathological examination...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28433371/in%C3%A2-vitro-fertilization-with-preimplantation-genetic-diagnosis-for-aneuploidies-in-advanced-maternal-age-a-randomized-controlled-study
#9
Carmen Rubio, José Bellver, Lorena Rodrigo, Gema Castillón, Alfredo Guillén, Carmina Vidal, Juan Giles, Marcos Ferrando, Sergio Cabanillas, José Remohí, Antonio Pellicer, Carlos Simón
OBJECTIVE: To determine the clinical value of preimplantation genetic diagnosis for aneuploidy screening (PGD-A) in women of advanced maternal age (AMA; between 38 and 41 years). DESIGN: This was a multicenter, randomized trial with two arms: a PGD-A group with blastocyst transfer, and a control group with blastocyst transfer without PGD-A. SETTING: Private reproductive centers. PATIENT(S): A total of 326 recruited patients fit the inclusion criteria, and 205 completed the study (100 in the PGD-A group and 105 in the control group)...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28425233/comparison-of-the-standard-gnrh-antagonist-protocol-and-the-luteal-phase-estradiol-gnrh-antagonist-priming-protocol-in-poor-ovarian-responders
#10
Mehmet Fırat Mutlu, İlknur Mutlu, Mehmet Erdem, İsmail Güler, Ahmet Erdem
BACKGROUND/AIM: The aim of the study was to compare the luteal estradiol patch/GnRH antagonists priming protocol (LPP) with the standard GnRH antagonist protocol in poor ovarian responders (PORs) in terms of the outcomes of in vitro fertilization (IVF) treatment. MATERIALS AND METHODS: IVF outcomes of 265 cycles in 265 patients (106 in the LPP group, 159 in the standard GnRH antagonist group) were evaluated retrospectively. RESULTS: Mean length of stimulation (11...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28423889/withdrawn-polymorphism-of-bola-drb3-gene-and-its-association-with-embryo-development-after-in-vitro-fertilization-in-slovak-spotted-x-holstein-fresian-crossbred-cattle
#11
Dominika Kułaj, Joanna Pokorska, Alexander Makarevič, Lucia Olexikova, Joanna Makulska, Peter Chrenek
Ahead of Print article withdrawn by publisher.
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28422964/effect-of-women-s-age-on-embryo-morphology-cleavage-rate-and-competence-a-multicenter-cohort-study
#12
MULTICENTER STUDY
Marie Louise Grøndahl, Sofie Lindgren Christiansen, Ulrik Schiøler Kesmodel, Inge Errebo Agerholm, Josephine Gabriela Lemmen, Peter Lundstrøm, Jeanette Bogstad, Morten Raaschou-Jensen, Steen Ladelund
This multicenter cohort study on embryo assessment and outcome data from 11,744 IVF/ICSI cycles with 104,830 oocytes and 42,074 embryos, presents the effect of women's age on oocyte, zygote, embryo morphology and cleavage parameters, as well as cycle outcome measures corrected for confounding factors as center, partner's age and referral diagnosis. Cycle outcome data confirmed the well-known effect of women's age. Oocyte nuclear maturation and proportion of 2 pro-nuclear (2PN) zygotes were not affected by age, while a significant increase in 3PN zygotes was observed in both IVF and ICSI (p<0...
2017: PloS One
https://www.readbyqxmd.com/read/28420519/simultaneous-cul-de-sac-and-tubal-pregnancy-following-in%C3%A2-vitro-fertilization
#13
Cheng-En Hsieh, Yuh-Ming Hwu, Sheng-Hsiang Li, Robert Kuo-Kuang Lee
OBJECTIVE: The aim of this study is to share a valuable experience of heterotopic pregnancy following in vitro fertilization. CASE REPORT: A 37-year-old, gravida 3, para 2 (cesarean section 2 times), woman underwent in vitro fertilization with three embryos transferred. On Day 23 after the embryo transfer, right tubal pregnancy with a 0.7-cm gestational sac was found by ultrasound, and her serum β-human chorionic gonadotropin level was 81,388 mIU/mL. She underwent a laparotomy with right salpingectomy...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28417348/elevated-progesterone-and-its-impact-on-birth-weight-after-fresh-embryo-transfers
#14
Yetunde Ibrahim, Miriam J Haviland, Michele R Hacker, Alan S Penzias, Kim L Thornton, Denny Sakkas
PURPOSE: The purpose of the study was to examine the association between serum progesterone levels on the day of hCG administration and birth weight among singleton live births after fresh embryo transfer. METHODS: This study was conducted as a retrospective cohort database analysis on patients who underwent IVF treatment cycles from January 2004 to April 2012. The study was performed at a University affiliated private infertility practice. All cycles that had achieved a singleton live birth after fresh embryo transfer and for which progesterone was measured on the day of hCG administration were examined...
April 17, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28413451/differential-dna-methylation-at-conserved-non-genic-elements-and-evidence-for-transgenerational-inheritance-following-developmental-exposure-to-mono-2-ethylhexyl-phthalate-and-5-azacytidine-in-zebrafish
#15
Jorke H Kamstra, Liana Bastos Sales, Peter Aleström, Juliette Legler
BACKGROUND: Exposure to environmental stressors during development may lead to latent and transgenerational adverse health effects. To understand the role of DNA methylation in these effects, we used zebrafish as a vertebrate model to investigate heritable changes in DNA methylation following chemical-induced stress during early development. We exposed zebrafish embryos to non-embryotoxic concentrations of the biologically active phthalate metabolite mono(2-ethylhexyl) phthalate (MEHP, 30 µM) and the DNA methyltransferase 1 inhibitor 5-azacytidine (5AC, 10 µM)...
2017: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/28412990/influence-of-pre-storage-incubation-on-hatchability-traits-thyroid-hormones-antioxidative-status-and-immunity-of-newly-hatched-chicks-at-two-chicken-breeder-flock-ages
#16
T A Ebeid, F A Twfeek, M H Assar, A M Bealish, R E Abd El-Karim, M Ragab
Egg storage longer than 7 days is associated with negative effects on hatchability traits. Pre-storage incubation has been a suggested method to reduce the negative effects of long-term storage times by enhancing the developmental stage of the embryo and probably reducing the embryonic stress. The objective of the present study was to investigate the effects of pre-storage incubation and storage time on hatchability characteristics, chick quality and serum thyroid hormones, antioxidative properties and immunoglobulin Y (IgY) concentrations of newly hatched chicks at two breeder flock ages...
April 17, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28412670/influences-of-granulocyte-growth-factor-in-uterine-perfusion-on-pregnancy-outcome-of-patients-with-failure-of-embryo-implantation-for-unknown-reason
#17
Jun He, Juan Liu, Hua Zhou, Chao Jun Chen
To investigate the influence of granulocyte growth factor in uterine perfusion on the pregnancy outcome of patients with failure of embryo implantation for unknown reason. Then, 68 patients with failure of embryo implantation for unknown reason were enrolled in our hospital from November 2013 to February 2015, which were divided into observation group and control group by random (34 patients in each group). Patients in observation group received basic treatment for granulocyte growth factor in uterine perfusion on the next day, while patients in control group received basic treatment with placebo...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28412632/seed-dormancy-and-germination-of-the-medicinal-holoparasitic-plant-cistanche-deserticola-from-the-cold-desert-of-northwest-china
#18
Jia Wang, Jerry M Baskin, Carol C Baskin, Guofang Liu, Xuejun Yang, Zhenying Huang
Cistanche deserticola is a holoparasitic plant with high medicinal value that reproduces only by seeds. However, the requirements for seed dormancy break and germination of this species remain unclear. The freshly matured dust-like seeds consist of a water-permeable seed coat and an undifferentiated oval-shaped embryo embedded in endosperm. No fresh seeds germinated in water or a 10(-5) M fluridone solution at any incubation temperature within 60 days. Length of embryos in seeds incubated in warm- and cold-started stratification sequences had increased 10...
April 8, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28412087/pregestational-type-2-diabetes-mellitus-induces-cardiac-hypertrophy-in-the-murine-embryo-through-cardiac-remodeling-and-fibrosis
#19
Xue Lin, Penghua Yang, E Albert Reece, Peixin Yang
BACKGROUND: Cardiac hypertrophy is highly prevalent in patients with type 2 diabetes mellitus (T2DM). Experimental evidence has implied that pregnant women with T2DM and their children are at an increased risk of cardiovascular diseases. Our previous mouse model study has revealed that maternal T2DM induces structural heart defects in their offspring. OBJECTIVE: The present study aims to determine whether maternal T2DM induces embryonic heart hypertrophy in a murine model of diabetic embryopathy...
April 12, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28407839/-effect-of-histone-acetylation-deacetylation-imbalances-on-key-gene-of-planar-cell-polarity-pathway
#20
Hong-Yu Duan, Yi Zhang, Kai-Yu Zhou, Chuan Wang, DA-Jian Qiu, Yi-Min Hua
OBJECTIVE: To investigate the effect of histone acetylation/deacetylation imbalances on embryonic hearts of mice and its effect on key genes of planar cell polarity (PCP) pathway-Vangl2, Scrib and Rac1 in H9C2 cells. METHODS: Forty pregnant C57/B6 mice were randomly assigned into three groups: blank group (n=10), vehicle group (n=10), and valproic acid (VPA)-treated group (n=20). In the VPA-treated group, VPA, a histone deacetylase (HDAC) inhibitor, was administered to each individual dam intraperitoneally at a single dose of 700 mg/kg on embryonic day 10...
April 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
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