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https://www.readbyqxmd.com/read/28543986/a-case-of-primordial-odontogenic-tumor-a-new-entity-in-the-latest-who-classification-2017
#1
Toshinori Ando, Madhu Shrestha, Takashi Nakamoto, Kaori Uchisako, Sachiko Yamasaki, Koichi Koizumi, Ikuko Ogawa, Mutsumi Miyauchi, Takashi Takata
Primordial odontogenic tumor (POT) is a rare lesion in the jaw which has been included as a new entity of benign mixed epithelial and mesenchymal odontogenic tumour in the latest World Health Organization (WHO) classification (2017). Only seven cases have been reported. It typically occurs in the posterior mandible. We report an additional case of POT in the maxilla of an 8-year-old girl presenting with an asymptomatic buccal enlargement. A well-defined, unilocular, radiolucent lesion was observed radiographically...
May 25, 2017: Pathology International
https://www.readbyqxmd.com/read/28540429/identification-of-markers-for-quiescent-pancreatic-stellate-cells-in-the-normal-human-pancreas
#2
Michael Friberg Bruun Nielsen, Michael Bau Mortensen, Sönke Detlefsen
Pancreatic stellate cells (PSCs) play a central role as source of fibrogenic cells in pancreatic cancer and chronic pancreatitis. In contrast to quiescent hepatic stellate cells (qHSCs), a specific marker for quiescent PSCs (qPSCs) that can be used in formalin-fixed and paraffin embedded (FFPE) normal human pancreatic tissue has not been identified. The aim of this study was to identify a marker enabling the identification of qPSCs in normal human FFPE pancreatic tissue. Immunohistochemical (IHC), double-IHC, immunofluorescence (IF) and double-IF analyses were carried out using a tissue microarray consisting of cores with normal human pancreatic tissue...
May 25, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28538193/ethyl-pyruvate-protects-pc12-cells-from-oxygen-glucose-deprivation-a-potential-role-in-ischemic-cerebrovascular-disease
#3
Wenbo Li, Jiyu Lou, Liping Wei, Hongying Bai, Yaohui Zhang, Yuqing He
The protective potential of ethyl pyruvate (EP) on neuron has been investigated previously. This study was intended to investigate the effects of EP on the severity of oxygen-glucose deprivation (OGD)-induced injury in neural-like PC12 cells. PC12 cells were exposed to OGD condition with or without EP treatment. Then, cell viability, apoptosis, and the expressions of neurotrophic factors were detected. Further, Sprague-Dawley rats were intravenously administered with 5mg/kg EP for 14 days post-middle cerebral artery occlusion (MCAO)...
May 21, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28534946/differentiation-of-nestin%C3%A2-negative-human-hair-follicle-outer-root-sheath-cells-into-neurons-in%C3%A2-vitro
#4
Wei Wu, Xiao-Li Wu, Yu-Qing Ji, Zhen Gao
A specialized quiescent population of hair follicle stem cells, residing in the hair follicle outer root sheath cells (ORSCs), has previously demonstrated pluripotency for differentiation into neural stem cells (NSCs). A previous study indicated that nestin‑positive hair follicle ORSCs are able to differentiate into neurons. However, little has been reported on the isolation of nestin‑negative human ORSCs and whether they can successfully differentiate into neurons in vitro. In the present study, nestin‑positive ORSCs were significantly reduced with a prolonged incubation time in vitro...
May 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28532017/effects-of-pore-orientation-on-in-vitro-properties-of-retinoic-acid-loaded-plga-gelatin-scaffolds-for-artificial-peripheral-nerve-application
#5
Farnaz Ghorbani, Ali Zamanian, Hanieh Nojehdehian
Different manufacturing processes of scaffolds affect their main properties by altering the microstructure of pores. In this study, poly (lactic-co-glycolic acid) (PLGA) scaffolds were synthesized by both freeze casting and freeze drying methods. Scanning electron microscopy (SEM) micrographs demonstrated a unidirectional microstructure in the freeze-cast and a number of random pores in the freeze-dried scaffolds. According to the results, the formation of a lamellar microstructure increased the mechanical strength, swelling ratio, biodegradation behavior and drug release level...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28524001/brain-derived-neurotrophic-factor-promotes-growth-of-neurons-and-neural-stem-cells-possibly-by-triggering-the-phosphoinositide-3-kinase-akt-glycogen-synthase-kinase-3%C3%AE-%C3%AE-catenin-pathway
#6
Jin-Wei Yang, Zhang Liang, Shi-Kang Deng Deng, Wei Ma, Xian-Bin Wang, Xing-Tong Li, Tong-Tong Wang, Yun-Fei Dai, Jian-Hui Guo, Li-Yan Li
BACKGROUND: Brain-derived neurotrophic factor (BDNF) plays a crucial role in promoting survival and differentiation of neurons and neural stem cells (NSCs), but the downstream regulating mechanisms remain poorly understood. OBJECTIVE: We investigated whether BDNF exerts its effect by triggering the phosphoinositide 3-kinase (PI3K), protein kinase B, PKB (AKT), glycogen synthase kinase-3β (GSK-3β) and β-catenin signaling pathway in cultured neurons and NSCs derived from the rat embryonic spinal cord...
May 18, 2017: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/28516286/establishment-of-a-tumor-sphere-cell-line-from-a-metastatic-brain-neuroendocrine-tumor
#7
Ryoichi Iwata, Masato Maruyama, Tomoki Ito, Yosuke Nakano, Yonehiro Kanemura, Taro Koike, Souichi Oe, Kunikazu Yoshimura, Masahiro Nonaka, Shosaku Nomura, Tetsuo Sugimoto, Hisao Yamada, Akio Asai
Neuroendocrine tumors are rare, and little is known about the existence of cancer stem cells in this disease. Identification of the tumorigenic population will contribute to the development of effective therapies targeting neuroendocrine tumors. Surgically resected brain metastases from a primary neuroendocrine tumor of unknown origin were dissociated and cultured in serum-free neurosphere medium. Stem cell properties, including self-renewal, differentiation potential, and stem cell marker expression, were examined...
May 17, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28515423/mir-520b-as-a-novel-molecular-target-for-suppressing-stemness-phenotype-of-head-neck-cancer-by-inhibiting-cd44
#8
Ya-Ching Lu, Ann-Joy Cheng, Li-Yu Lee, Guo-Rung You, Yan-Liang Li, Hsin-Ying Chen, Joseph T Chang
Cancer stem cells preferentially acquire the specific characteristics of stress tolerance and high mobility, allowing them to progress to a therapy-refractive state. To identify a critical molecule to regulate cancer stemness is indispensable to erratically cure cancer. In this study, we identified miR-520b as a novel molecular target to suppress head-neck cancer (HNC) with stemness phenotype. MiR-520b inhibited cellular migration and invasion via the mechanism of epithelial-mesenchymal transition. It also sensitized cells to therapeutic drug and irradiation...
May 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28512471/resveratrol-induces-differentiation-of-human-umbilical-cord-mesenchymal-stem-cells-into-neuron-like-cells
#9
Li Guo, Liang Wang, Li Wang, Shi Yun-Peng, Jing-Jing Zhou, Zongmao Zhao, De-Pei Li
Objective. Human umbilical cord mesenchymal stem cells (hUC-MSCs) potentially differentiate to various types of cells including neuron-like cells. The natural polyphenol resveratrol benefits patients with many diseases including ischemic brain injury. We hypothesize that resveratrol induces differentiation of hUC-MSCs into neuron-like cells. Methods. Flow cytometry was used to determine the surface antigens in different stage of hUC-MSCs (P2, P5, and P10). Nestin, neuron-specific enolase (NSE), and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry, Western blotting, and real time RT-PCT...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28508891/joint-morphogenetic-cells-in-the-adult-mammalian-synovium
#10
Anke J Roelofs, Janja Zupan, Anna H K Riemen, Karolina Kania, Sharon Ansboro, Nathan White, Susan M Clark, Cosimo De Bari
The stem cells that safeguard synovial joints in adulthood are undefined. Studies on mesenchymal stromal/stem cells (MSCs) have mainly focused on bone marrow. Here we show that lineage tracing of Gdf5-expressing joint interzone cells identifies in adult mouse synovium an MSC population largely negative for the skeletal stem cell markers Nestin-GFP, Leptin receptor and Gremlin1. Following cartilage injury, Gdf5-lineage cells underpin synovial hyperplasia through proliferation, are recruited to a Nestin-GFP(high) perivascular population, and contribute to cartilage repair...
May 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/28507480/electro-acupuncture-promotes-the-proliferation-of-neural-stem-cells-and-the-survival-of-neurons-by-downregulating-mir-449a-in-rat-with-spinal-cord-injury
#11
Yi Zhu, Yaochi Wu, Rong Zhang
The aim of this study is to investigate the mechanism of electro-acupuncture (EA) on the recovery of injured spinal cord. Rats were randomly divided into normal control, sham-operated, SCI, SCI+EA group and T10 segment spinal cord injury (SCI) rat model was established by the modified Allen's method. After 7 days, the mRNA and protein expression of Nestin, neuron specific nuclear protein (NeuN) and calcitonin gene related peptide (CGRP) were detected by real time RT-PCR, Western blot and immunohistochemistry respectively...
2017: EXCLI journal
https://www.readbyqxmd.com/read/28502321/cytomegalovirus-in-the-human-dentate-gyrus-and-its-impact-on-neural-progenitor-cells-report-of-two-cases
#12
Ju-Yoon Yoon, Brett Danielson, Derek Mathis, Jason Karamchandani, David G Munoz
Congenital cytomegalovirus (CMV) infection in the 2nd and 3rd trimester results in catastrophic CNS abnormalities. This susceptibility is thought to result from the high proportion of neural stem cells in the developing brain. In immunocompromised adults, CNS infection by CMV preferentially affects ependymal surfaces, from where it expands to involve the parenchyma. Experimental models of murine CMV infection demonstrate viral tropism for the dentate gyrus (DG) and subventricular zone, the areas in which adult neurogenesis occurs...
May 15, 2017: Clinical Neuropathology
https://www.readbyqxmd.com/read/28500734/differentiation-of-human-olfactory-bulb-derived-neural-stem-cell-towards-oligodendrocyte-development
#13
H E Marei, Z Shouman, A Althani, N Afifi, A Abd-Elmaksoud, S Lashen, A Hasan, T Caceci, R Rizzi, C Cenciarelli, P Casalbore
In the central nervous system, oligodendrocytes are the glial element in charge of myelin formation. Obtaining an overall presence of oligodendrocyte precursor cells/oligodendrocytes. (OPCs/OLs) in culture from different sources of NSCs is an important research area, because OPCs/OLs may provide a promising therapeutic strategy for diseases affecting myelination of axons. The present study was designed to differentiate human olfactory bulb NSCs (OBNSCs) into OPCs/OLs and using expression profiling (RT-qPCR) gene, immunocytochemistry and specific protein expression to highlight molecular mechanism(s) underlying differentiation of human OBNSCs into OPCs/OLs...
May 13, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28500556/reversing-glioma-malignancy-a-new-look-at-the-role-of-antidepressant-drugs-as-adjuvant-therapy-for-glioblastoma-multiforme
#14
Anna M Bielecka-Wajdman, Marta Lesiak, Tomasz Ludyga, Aleksander Sieroń, Ewa Obuchowicz
PURPOSE: The role of glioma stem cells (GSCs) in cancer progression is currently debated; however, it is hypothesised that this subpopulation is partially responsible for therapeutic resistance observed in glioblastoma multiforme (GBM). Recent studies have shown that the current treatments not only fail to eliminate the GSC population but even promote GSCs through reprogramming of glioma non-stem cells to stem cells. Since the standard GBM treatment often requires supplementation with adjuvant drugs such as antidepressants, their role in the regulation of the heterogeneous nature of GSCs needs evaluation...
June 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28500065/loss-of-mpdz-impairs-ependymal-cell-integrity-leading-to-perinatal-onset-hydrocephalus-in-mice
#15
Anja Feldner, M Gordian Adam, Fabian Tetzlaff, Iris Moll, Dorde Komljenovic, Felix Sahm, Tobias Bäuerle, Hiroshi Ishikawa, Horst Schroten, Thomas Korff, Ilse Hofmann, Hartwig Wolburg, Andreas von Deimling, Andreas Fischer
Hydrocephalus is a common congenital anomaly. LCAM1 and MPDZ (MUPP1) are the only known human gene loci associated with non-syndromic hydrocephalus. To investigate functions of the tight junction-associated protein Mpdz, we generated mouse models. Global Mpdz gene deletion or conditional inactivation in Nestin-positive cells led to formation of supratentorial hydrocephalus in the early postnatal period. Blood vessels, epithelial cells of the choroid plexus, and cilia on ependymal cells, which line the ventricular system, remained morphologically intact in Mpdz-deficient brains...
May 12, 2017: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/28499977/human-neural-stem-progenitor-cells-derived-from-the-olfactory-epithelium-express-the-trkb-receptor-and-migrate-in-response-to-bdnf
#16
Leonardo Ortiz-López, Jorge Julio González-Olvera, Nelly Maritza Vega-Rivera, Maria García-Anaya, Ana Karen Carapia-Hernández, Julio César Velázquez-Escobar, Gerardo Bernabé Ramírez-Rodríguez
Neurogenesis constitutively occurs in the olfactory epithelium of mammals, including humans. The fact that new neurons in the adult olfactory epithelium derive from resident neural stem/progenitor cells suggests a potential use for these cells in studies of neural diseases, as well as in neuronal cell replacement therapies. In this regard, some studies have proposed that the human olfactory epithelium is a source of neural stem/progenitor cells for autologous transplantation. Although these potential applications are interesting, it is important to understand the cell biology and/or whether human neural stem/progenitor cells in the olfactory epithelium sense external signals, such as brain-derived neurotrophic factor (BDNF), that is also found in other pro-neurogenic microenvironments...
May 10, 2017: Neuroscience
https://www.readbyqxmd.com/read/28498445/evaluation-of-expression-of-cancer-stem-cell-markers-and-fusion-gene-in-synovial-sarcoma-insights-into-histogenesis-and-pathogenesis
#17
Yang Zhou, Dongdong Chen, Yan Qi, Ruixue Liu, Shugang Li, Hong Zou, Jiaojiao Lan, Xinxin Ju, Jinfang Jiang, Weihua Liang, Yaoyuan Shen, Lijuan Pang, Feng Li
Synovial sarcoma (SS) is an aggressive soft tissue tumor, with uncertain histological and cellular origin. SYT-SSX is considered to be responsible for sarcoma initiation and progression. The histogenesis and pathogenesis of this tumor are poorly understood, and prognosis of patients of SS is unsatisfactory. Recent studies have shown an association of cancer stem cells with the initiation and development of tumors. We explored immunohistochemical expression level of stem cell associated markers to determine the possible histogenesis and pathogenesis of SS...
May 2, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28493828/overexpression-of-leptin-receptor-in-human-glioblastoma-correlation-with-vasculogenic-mimicry-and-poor-prognosis
#18
Guosheng Han, Yanan Li, Yiqun Cao, Zhijian Yue, Yuhui Zhang, Laixing Wang, Jianmin Liu
Vasculogenic mimicry (VM) was an important tumor blood supply to complement the endothelial cell-dependent angiogenesis, while leptin and receptor (ObR) involved in angiogenesis in glioblastoma has been reported on previous study, but the relationship between ObR expression and VM formation in human glioblastoma tissues, as well as their prognostic significance still remains unclear. In our study, we found that VM recognized by CD31-/PAS+ immunohistochemical staining in glioblastoma tissues showed a positive correlation with leptin expression (r = 0...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28487338/poststroke-sonic-hedgehog-agonist-treatment-improves-functional-recovery-by-enhancing-neurogenesis-and-angiogenesis
#19
Yongming Jin, Austin Barnett, Yifan Zhang, Xin Yu, Yu Luo
BACKGROUND AND PURPOSE: Because of the limitation in treatment window of the r-tPA (recombinant tissue-type plasminogen activator), the development of delayed treatment for stroke is needed. In this study, we examined the efficacy of delayed poststroke treatment (post 3-8 days) of the sonic hedgehog pathway agonist on functional recovery and the underlying mechanisms. METHODS: We evaluated functional recovery at 1 month after stroke using locomotion analysis and Barnes maze test for cognitive function...
May 9, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28480968/the-influence-of-raav2-mediated-sox2-delivery-into-neonatal-and-adult-human-rpe-cells-a-comparative-study
#20
Razie Ezati, Azadeh Etemadzadeh, Zahra-Soheila Soheili, Shahram Samiei, Ehsan Ranaei Pirmardan, Malihe Davari, Hoda Shams Najafabadi
Cell replacement is a promising therapy for degenerative diseases like age-related macular degeneration (AMD). Since the human retina lacks regeneration capacity, much attention has been directed towards persuading for cells that can differentiate into retinal neurons. In this report we have investigated reprogramming of the human RPE cells and concerned the effect of donor age on the cellular fate as a critical determinant in reprograming competence. We evaluated the effect of SOX2 over-expression in human neonatal and adult RPE cells in cultures...
May 8, 2017: Journal of Cellular Physiology
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