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https://www.readbyqxmd.com/read/28448522/nestin-expression-is-upregulated-in-the-fibrotic-rat-heart-and-is-localized-in-collagen-expressing-mesenchymal-cells-and-interstitial-cd31-cells
#1
Vanessa Hertig, Kim Tardif, Marc Andre Meus, Natacha Duquette, Louis Villeneuve, Fanny Toussaint, Jonathan Ledoux, Angelino Calderone
Renal and lung fibrosis was characterized by the accumulation of collagen-immunoreactive mesenchymal cells expressing the intermediate filament protein nestin. The present study tested the hypothesis that nestin expression was increased in the hypertrophied/fibrotic left ventricle of suprarenal abdominal aorta constricted adult male Sprague-Dawley rats and induced in ventricular fibroblasts by pro-fibrotic peptide growth factors. Nestin protein levels were upregulated in the pressure-overloaded left ventricle and expression positively correlated with the rise of mean arterial pressure...
2017: PloS One
https://www.readbyqxmd.com/read/28447764/sevoflurane-anesthesia-in-pregnant-rats-negatively-affects-nerve-function-in-offspring-potentially-via-inhibition-of-the-wnt-%C3%AE-catenin-pathway
#2
Yiyao Wang, Yu Li, Qunzhi Xing, Xuechan G Han, Xu Dong, Yiping Lu, Mintao Zhou
Due to the rapid development of medical technology used to perform intrauterine procedures during pregnancy, the number of patients receiving fetal surgery under general anesthesia is increasing. The aim of the present study was to determine the effects of anesthetics on the offspring of rats, and to identify the potential mechanisms underlying these effects. On day 14 of pregnancy, Sprague‑Dawley rats were equally divided into the following 3 groups (n=9): Control group (n=3), 3% sevoflurane group (n=3) and 4% sevoflurane group (n=3)...
March 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28442243/abnormal-neural-precursor-cell-regulation-in-the-early-postnatal-fragile-x-mouse-hippocampus
#3
Mary Sourial, Laurie C Doering
The regulation of neural precursor cells (NPCs) is indispensable for a properly functioning brain. Abnormalities in NPC proliferation, differentiation, survival, or integration have been linked to various neurological diseases including Fragile X syndrome. Yet, no studies have examined NPCs from the early postnatal Fragile X mouse hippocampus despite the importance of this developmental time point, which marks the highest expression level of FMRP, the protein missing in Fragile X, in the rodent hippocampus and is when hippocampal NPCs have migrated to the dentate gyrus (DG) to give rise to lifelong neurogenesis...
April 22, 2017: Brain Research
https://www.readbyqxmd.com/read/28441579/geldanamycin-inspired-compounds-induce-direct-trans-differentiation-of-human-mesenchymal-stem-cells-to-neurons
#4
Srinivas Jogula, Anand Ram Soorneedi, Jagan Gaddam, Srinivas Chamakuri, Girdhar Singh Deora, Ranjith Kumar Indarapu, Murali Krishna Ramgopal, Subhadra Dravida, Prabhat Arya
Inspired from geldanamycin, the synthesis of a new series of 20-membered macrocyclic compounds is developed. The key features in our design are (i) retention of the fragment having the precise chiral functional groups of geldanamycin at C10, C11, C12 and C14, and (ii) replacement of an olefin moiety with the ester group, and the quinoid sub-structure with the triazole ring. The southern fragment needed for the macrocyclic ring formation was obtained from Evans' syn aldol as the key reaction and with the use of D-mannitol as the cheap source of a chiral starting material...
April 18, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28439082/expression-of-nestin-associates-with-brca1-mutations-a-basal-like-phenotype-and-aggressive-breast-cancer
#5
Kristi Krüger, Elisabeth Wik, Gøril Knutsvik, Hawa Nalwoga, Tor A Klingen, Jarle B Arnes, Ying Chen, Monica Mannelqvist, Konstantina Dimitrakopoulou, Ingunn M Stefansson, Even Birkeland, Turid Aas, Nicholas P Tobin, Inge Jonassen, Jonas Bergh, William D Foulkes, Lars A Akslen
We here examined whether Nestin, by protein and mRNA levels, could be a predictor of BRCA1 related breast cancer, a basal-like phenotype, and aggressive tumours. Immunohistochemical staining of Nestin was done in independent breast cancer hospital cohorts (Series I-V, total 1257 cases). Also, TCGA proteomic data (n = 103), mRNA microarray data from TCGA (n = 520), METABRIC (n = 1992), and 6 open access breast cancer datasets (n = 1908) were analysed. Patients with Nestin protein expression in tumour cells more often had BRCA1 germline mutations (OR 8...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28438578/global-cerebral-ischemia-in-rats-leads-to-amnesiadue-to-selective-neuronal-death-followed-by-astroglial-scar-formationin-the-ca1-layer
#6
Kevin Sadelli, Jean-Claude Stamegna, Stéphane D Girard, Nathalie Baril, Guy Escoffier, Maïna Brus, Antoine D Véron, Michel Khrestchatisky, François S Roman
Global Cerebral Ischemia (GCI) occurs following cardiac arrest or neonatal asphyxia and leads to harmful neurological consequences. In most cases, patients who survive cardiac arrest develop severe cognitive and motor impairments. This study focused on learning and memory deficits associated with brain neuroanatomical reorganization that appears after GCI. The four-vessel occlusion (4VO) model was performed to produce a transient GCI. Hippocampal lesions in ischemic rats were visualized using anatomical Magnetic Resonance Imaging (aMRI)...
April 21, 2017: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/28436114/all-trans-retinoic-acid-stimulates-overexpression-of-tumor-protein-d52-tpd52-isoform-3-and-neuronal-differentiation-of-imr-32-cells
#7
Sudha Sravanti Kotapalli, Chandrashekhar Dasari, Divya Duscharla, Karthik Reddy Kami Reddy, Manjula Kasula, Ramesh Ummanni
Tumor protein D52 (TPD52), a proto-oncogene is overexpressed in a variety of epithelial carcinomas and plays an important role in cell proliferation, migration and cell death. In the present study we found that the treatment of IMR-32 neuroblastoma (NB) cells with retinoic acid (RA) stimulates an increase in expression of TPD52. TPD52 expression is detectable after 72 h, can be maintained till differentiation of NB cells suggesting that TPD52 is involved in differentiation. Here, we demonstrate that TPD52 is essential for RA to promote differentiation of NB cells...
April 24, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28434161/can-valproic-acid-regulate-neurogenesis-from-nestin-cells-in-the-adult-midbrain
#8
Parisa Farzanehfar, Malcolm K Horne, Tim D Aumann
Degeneration of dopamine (DA) neurons in the substantia nigra pars compacta (SNc) causes the motor symptoms (e.g. tremor, muscle rigidity, bradykinesia, postural instability) of Parkinson's disease (PD). It is generally agreed that replacing these neurons will provide better motor symptom relief and fewer side effects than current pharmacotherapies. One potential approach to this is up-regulating endogenous DA neurogenesis in SNc. In the present study, we conducted bioinformatics analyses to identify signalling pathways that control expression of Pax6 and Msx1 genes, which have been identified as potentially important neurogenic regulators in the adult midbrain...
April 22, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28432079/regulation-of-microtubule-associated-motors-drives-intermediate-filament-network-polarization
#9
Cécile Leduc, Sandrine Etienne-Manneville
Intermediate filaments (IFs) are key players in the control of cell morphology and structure as well as in active processes such as cell polarization, migration, and mechanoresponses. However, the regulatory mechanisms controlling IF dynamics and organization in motile cells are still poorly understood. In this study, we investigate the mechanisms leading to the polarized rearrangement of the IF network along the polarity axis. Using photobleaching and photoconversion experiments in glial cells expressing vimentin, glial fibrillary acidic protein, and nestin, we show that the distribution of cytoplasmic IFs results from a continuous turnover based on the cooperation of an actin-dependent retrograde flow and anterograde and retrograde microtubule-dependent transports...
April 21, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28428031/the-effect-of-hypoxia-preconditioning-on-the-neural-and-stemness-genes-expression-profiling-in-human-umbilical-cord-blood-mesenchymal-stem-cells
#10
Maryam Kheirandish, Somaie Piri Gavgani, Shahram Samiee
In recent years, human umbilical cord blood-derived mesenchymal stem cell (hUB-MSCs) has been regarded as an alternative source for stem cell therapy. In this study, we evaluated the effect of hypoxia preconditioning (HPC) on the expression of Nt-3, GFAP, Nestin, Oct-4 and Nanog genes and proliferative capacity of hUB-MSCs in comparison with normoxic conditions. HPC+Hypoxia protocol includes cultured hUB-MSCs for 15min at 2.5% O2 and after that reoxygenation for 30min at 21% O2 (HPC), and then hypoxia preconditioned hUB-MSCs subjected to 2...
March 31, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28427858/mice-with-conditional-neurod1-knockout-display-reduced-aberrant-hippocampal-neurogenesis-but-no-change-in-epileptic-seizures
#11
Rebecca Brulet, Jingfei Zhu, Mahafuza Aktar, Jenny Hsieh, Kyung-Ok Cho
Adult neurogenesis is significantly increased in the hippocampus of rodent models of temporal lobe epilepsy (TLE). These adult-generated neurons have recently been shown to play a contributing role in the development of spontaneous recurrent seizures (SRS). In order to eventually target pro-epileptic adult neurogenesis in the clinical setting, it will be important to identify molecular players involved in the control of aberrant neurogenesis after seizures. Here, we focused on NeuroD1 (ND1), a member of the bHLH family of transcription factors previously shown to play an essential role in the differentiation and maturation of adult-generated neurons in the hippocampus...
April 18, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28427209/hif1%C3%AE-regulates-single-differentiated-glioma-cell-dedifferentiation-to-stem-like-cell-phenotypes-with-high-tumorigenic-potential-under-hypoxia
#12
Pan Wang, Chuan Lan, Shuanglong Xiong, Xiuwen Zhao, You'an Shan, Rong Hu, Wenwu Wan, Shuangjiang Yu, Bin Liao, Guangzhi Li, Junwei Wang, Dewei Zou, Bing Chen, Hua Feng, Nan Wu
The standard treatment for Glioblastoma multiforme (GBM) is surgical resection and subsequent radiotherapy and chemotherapy. Surgical resection of GBM is typically restricted because of its invasive growth, which results in residual tumor cells including glioma stem cells (GSCs) and differentiated cells. Recurrence has been previously thought to occur as a result of these GSCs, and hypoxic microenvironment maintains the GSCs stemness also plays an important role. Summarizing traditional studies and we find many researchers ignored the influence of hypoxia on differentiated cells...
March 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420791/adult-enteric-nervous-system-in-health-is-maintained-by-a-dynamic-balance-between-neuronal-apoptosis-and-neurogenesis
#13
Subhash Kulkarni, Maria-Adelaide Micci, Jenna Leser, Changsik Shin, Shiue-Cheng Tang, Ya-Yuan Fu, Liansheng Liu, Qian Li, Monalee Saha, Cuiping Li, Grigori Enikolopov, Laren Becker, Nikolai Rakhilin, Michael Anderson, Xiling Shen, Xinzhong Dong, Manish J Butte, Hongjun Song, E Michelle Southard-Smith, Raj P Kapur, Milena Bogunovic, Pankaj J Pasricha
According to current dogma, there is little or no ongoing neurogenesis in the fully developed adult enteric nervous system. This lack of neurogenesis leaves unanswered the question of how enteric neuronal populations are maintained in adult guts, given previous reports of ongoing neuronal death. Here, we confirm that despite ongoing neuronal cell loss because of apoptosis in the myenteric ganglia of the adult small intestine, total myenteric neuronal numbers remain constant. This observed neuronal homeostasis is maintained by new neurons formed in vivo from dividing precursor cells that are located within myenteric ganglia and express both Nestin and p75NTR, but not the pan-glial marker Sox10...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28418837/rapamycin-promotes-differentiation-increasing-%C3%AE-iii-tubulin-neun-and-neurod-while-suppressing-nestin-expression-in-glioblastoma-cells
#14
Michela Ferrucci, Francesca Biagioni, Paola Lenzi, Stefano Gambardella, Rosangela Ferese, Maria Teresa Calierno, Alessandra Falleni, Alfonso Grimaldi, Alessandro Frati, Vincenzo Esposito, Cristina Limatola, Francesco Fornai
Glioblastoma cells feature mammalian target of rapamycin (mTOR) up-regulation which relates to a variety of effects such as: lower survival, higher infiltration, high stemness and radio- and chemo-resistance. Recently, it was demonstrated that mTOR may produce a gene shift leading to altered protein expression. Therefore, in the present study we administered different doses of the mTOR inhibitor rapamycin to explore whether the transcription of specific genes are modified. By using a variety of methods we demonstrate that rapamycin stimulates gene transcription related to neuronal differentiation while inhibiting stemness related genes such as nestin...
March 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28418164/molecular-subtyping-of-mammary-like-adenocarcinoma-of-the-vulva-shows-molecular-similarity-to-breast-carcinomas
#15
Basile Tessier-Cloutier, Karama Asleh-Aburaya, Varsha Shah, W Glenn McCluggage, Anna Tinker, C Blake Gilks
AIMS: Mammary-like adenocarcinoma (MLA) of the vulva is thought to be derived from vulvar mammary-like glands. We characterized a series of MLA using an immunohistochemical algorithm that identifies the major molecular subtypes of breast cancer. METHODS AND RESULTS: Seven cases of vulval MLA were stained for ER, PR, HER2, Ki-67, EGFR, CK5, nestin and INPP4b. 17 cases of vulval extramammary Paget disease (EMPD), 7 with invasion, were studied for comparison. The median age of patients with MLA was 72 years...
April 18, 2017: Histopathology
https://www.readbyqxmd.com/read/28416308/implantation-of-endothelial-cells-with-mesenchymal-stem-cells-accelerates-dental-pulp-tissue-regeneration-healing-in-pulpotomized-rat-molars
#16
Yukiko Sueyama, Tomoatsu Kaneko, Takafumi Ito, Reika Kaneko, Takashi Okiji
INTRODUCTION: This study aimed to examine whether the implantation of mesenchymal stem cells (MSCs) with endothelial cells (ECs) accelerates pulp tissue regeneration/healing and induces dentin bridge formation in a rat model of molar coronal pulp regeneration. METHODS: The maxillary first molars of Wistar rats were subjected to pulpotomy. Then, pulp chambers were implanted with biodegradable hydrogel-made scaffolds carrying MSCs together or without dermal microvascular ECs, and the cavities were sealed with mineral trioxide aggregate...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28415725/stat3-positive-tumor-cells-contribute-to-vessels-neoformation-in-primary-central-nervous-system-lymphoma
#17
Simona Ruggieri, Roberto Tamma, Nicoletta Resta, Francesco Albano, Nicoletta Coccaro, Daria Loconte, Tiziana Annese, Mariella Errede, Giorgina Specchia, Rebecca Senetta, Paola Cassoni, Domenico Ribatti, Beatrice Nico
With the aim of elucidating the relationship between Stat3 expression and tumor vessels abnormalities in the PCNLs, in this study we evaluated Stat3 and pStat3 expression by Real-time PCR and by immunohistochemistry in biopsy sections from PCNSL patients. Correlations of the expression levels with the presence of aberrant vessels were analyzed by confocal laser microscopy analysis, using FVIII as endothelial cell marker, CD133 and nestin as cancer stem cell (CSC) marker, CD20 as tumor cell marker, and Stat3...
March 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28411880/in%C3%A2-vitro-induction-effect-of-1-25-oh-2d3-on-differentiation-of-hair-follicle-stem-cell-into-keratinocyte
#18
Sanaz Joulai Veijouyeh, Farhad Mashayekhi, Abazar Yari, Fatemeh Heidari, Nayereh Sajedi, Fatemeh Moghani Ghoroghi, Maliheh Nobakht
BACKGROUND: Stem cells are characterized by self-renewal and differentiation capabilities. The bulge hair follicle stem cells (HFSCs) are able to convert to epithelial components. The active metabolite of vitamin D, 1,25(OH)2D3, plays important roles in this differentiation process. In the present study has found that 1,25(OH)2D3 induces the HFSCs differentiation into keratinocyte. METHODS: HFSCs are isolated from rat whiskers and cultivated in DMEM medium. To isolate bulge stem cell population, flow cytometry and immunocytochemistry using K15, CD34 and nestin biomarkers were performed...
February 2017: Biomedical Journal
https://www.readbyqxmd.com/read/28411401/expression-of-stem-cell-markers-and-dopamine-d2-receptors-in-human-and-rat-prolactinomas
#19
Zhichao Gao, Lin Cai, Jianglong Lu, Chengde Wang, Qun Li, Jian Chen, Xiaoxiao Song, Xianbin Chen, Linlin Zhang, Weiming Zheng, Zhipeng Su
BACKGROUND Dopamine agonists (DAs) are the first-line treatment for prolactinomas. DAs primarily target the dopamine D2 receptor (D2R). Tumor stem-like cells (TSLCs) are associated with the tolerance to radiotherapy and chemotherapy. TSLCs have also been identified in pituitary adenomas. We aimed to characterize the expression pattern of stem cell markers and D2R in human and rat prolactinomas. MATERIAL AND METHODS Human prolactinoma specimens (n=14) were obtained from patients with surgical resection. The xenograft model of rat prolactinomas was generated by endermically injecting MMQ cells, HE and PRL were confirmed by immunohistochemical staining of tumor sections, and the expression of serum PRL was measured by ELISA...
April 15, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28401575/new-insights-into-the-neural-differentiation-potential-of-canine-adipose-tissue-derived-mesenchymal-stem-cells
#20
D Blecker, M I Elashry, M Heimann, S Wenisch, S Arnhold
Adipose tissue-derived stem cells (ASCs) can be obtained from different adipose tissue sources within the body. It is an abundant cell pool, easily accessible, suitable for cultivation and expansion in vitro and preparation for therapeutic approaches. Amongst these therapeutic approaches are tissue engineering and nervous system disorders such as spinal cord injuries. For such treatment, ASCs have to be reliably differentiated in to the neuronal direction. Therefore, we investigated the neural differentiation potential of ASCs using protocols with neurogenic inductors such as valproic acid and forskolin, while dog brain tissue served as control...
April 11, 2017: Anatomia, Histologia, Embryologia
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