keyword
MENU ▼
Read by QxMD icon Read
search

Nestin

keyword
https://www.readbyqxmd.com/read/29468805/aligned-nanofiber-material-supports-cell-growth-and-increases-osteogenesis-in-canine-adipose-derived-mesenchymal-stem-cells-in-vitro
#1
Sony Pandey, Kusum Rathore, Jed Johnson, Maria Cekanova
Tissue engineering shows great promise for the treatment of degenerative diseases, including bone repair. Polymer nanofibers provide a three-dimensional (3-D) scaffold for attachment and growth of mesenchymal stem cells. Increasing evidence supports that fiber alignment on scaffolds plays a major role in the viability and differentiation of stem cells. We compared the cell viability of canine adipose tissue-derived mesenchymal stem cells (cADMSCs) cultured in the aligned- (NanoAligned™) and random- (NanoECM™) oriented polycaprolactone (PCL) nanofiber-coated plates to control polystyrene tissue culture plates using a proliferation assay...
February 22, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29468156/adherent-vs-free-floating-neural-induction-by-dual-smad-inhibition-for-neurosphere-cultures-derived-from-human-induced-pluripotent-stem-cells
#2
Martje G Pauly, Victor Krajka, Felix Stengel, Philip Seibler, Christine Klein, Philipp Capetian
Keeping neural stem cells under proliferation, followed by terminal differentiation, can substantially increase the number of neurons generated. With regard to the usability of proliferating neurospheres (NSPHs) cultures, adherent induction protocols have not yet been studied in comparison to embryoid body (EB)-based protocols. To compare these proctocols, neural induction of human induced pluripotent stem cells was performed by dual SMAD inhibition under both adherent and free-floating EB culture conditions...
2018: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/29459500/choroid-plexus-carcinoma-with-neuronal-and-glial-differentiation-in-a-7-week-old-male-sprague-dawley-rat
#3
Mari Inohana, Ayumi Eguchi, Misato Nakamura, Rei Nagahara, Yosuke Watanabe, Toshinori Yoshida, Makoto Shibutani
We describe a case of choroid plexus carcinoma arising in the cerebrum of a 7-week-old male Sprague-Dawley rat. The tumor mass occupied the right lateral ventricle of the cerebrum. Histological analyses revealed that the epithelial tumor cells had proliferated in tubular, cribriform, papillary and solid growth patterns in the vicinity of the choroid plexus, with slight invasion into the cerebrum parenchyma. We divided the tumor cells into cuboidal, elongated and intermediate cells. Immunohistochemical studies showed that these tumor cells expressed relatively high levels of cytokeratin AE1/AE3, vimentin and glial fibrillary acidic proteins, and low levels of nestin, oligodendrocyte transcription factor and doublecortin proteins...
February 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29457125/enhanced-differentiation-of-dental-pulp-cells-cultured-on-microtubular-polymer-scaffolds-in-vitro
#4
Morteza Haeri, Karen Sagomonyants, Mina Mina, Liisa T Kuhn, A Jon Goldberg
Dental caries (tooth decay) is the most common chronic disease. Dental tissue engineering is a promising alternative approach to alleviate the shortcomings of the currently available restorative materials. Mimicking the natural extracellular matrix (ECM) could enhance the performance of tissue engineering scaffolds. In this study, we developed microtubular (~20 μm diameter) polymethyl methacrylate (PMMA) scaffolds resembling the tubular (~2.5 μm diameter) structure of dentin, the collagen-based mineralized tissue that forms the major portion of teeth, to study the effect of scaffold architecture on differentiation of mouse dental pulp cells in vitro ...
June 2017: Regenerative Engineering and Translational Medicine
https://www.readbyqxmd.com/read/29449696/snail-regulates-bmp-and-tgf%C3%AE-pathways-to-control-the-differentiation-status-of-glioma-initiating-cells
#5
Laia Caja, Kalliopi Tzavlaki, Mahsa S Dadras, E-Jean Tan, Gad Hatem, Naga P Maturi, Anita Morén, Lotta Wik, Yukihide Watanabe, Katia Savary, Masood Kamali-Moghaddan, Lene Uhrbom, Carl-Henrik Heldin, Aristidis Moustakas
Glioblastoma multiforme is a brain malignancy characterized by high heterogeneity, invasiveness, and resistance to current therapies, attributes related to the occurrence of glioma stem cells (GSCs). Transforming growth factor β (TGFβ) promotes self-renewal and bone morphogenetic protein (BMP) induces differentiation of GSCs. BMP7 induces the transcription factor Snail to promote astrocytic differentiation in GSCs and suppress tumor growth in vivo. We demonstrate that Snail represses stemness in GSCs. Snail interacts with SMAD signaling mediators, generates a positive feedback loop of BMP signaling and transcriptionally represses the TGFB1 gene, decreasing TGFβ1 signaling activity...
February 16, 2018: Oncogene
https://www.readbyqxmd.com/read/29445893/nestin-expression-is-differently-regulated-between-odontoblasts-and-the-subodontoblastic-layer-in-mice
#6
Mitsushiro Nakatomi, Angela Quispe-Salcedo, Masaka Sakaguchi, Hiroko Ida-Yonemochi, Hideyuki Okano, Hayato Ohshima
The Nestin gene encodes type VI intermediate filament and is known to be expressed in undifferentiated cells during neurogenesis and myogenesis. To regulate Nestin expression, the first or second intron enhancer is activated in a tissue-dependent manner, for example, the former in mesodermal cells and the latter in neural stem cells. Although Nestin has also been used as a differentiation marker for odontoblasts during tooth development, how Nestin expression is regulated in odontoblasts remains unclear. Therefore, this study aimed to compare the expression patterns of Nestin-GFP (green fluorescent protein) with that of endogenous Nestin in developing teeth of Nestin-EGFP (enhanced GFP) transgenic mice, in which the second intron enhancer is connected with the EGFP domain, at postnatal 7d, 3w, and 8w...
February 14, 2018: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/29437010/neuronal-expression-of-junctional-adhesion-molecule-c-is-essential-for-retinal-thickness-and-photoreceptor-survival
#7
Y Li, F Zhang, W Lu, X Li
BACKGROUND: Photoreceptor cell death is a key pathology of retinal degeneration diseases. To date, the molecular mechanisms for this pathological process remain largely unclear. Junctional adhesion molecule-c (Jam-c) has been shown to play important roles in different biological events. However, its effect on retinal neuronal cells is unknown. OBJECTIVE: To determine the effect of Jam-c on adult mouse eyes, particularly, on retinal structure, vasculature and photoreceptor cells, in order to explore potential important target molecules for ocular diseases...
February 12, 2018: Current Molecular Medicine
https://www.readbyqxmd.com/read/29436662/comparison-of-the-properties-of-neural-stem-cells-of-the-hippocampus-in-the-tree-shrew-and-rat-in%C3%A2-vitro
#8
Yuan-Dong Hu, Qiong Zhao, Xue-Rong Zhang, Liu-Lin Xiong, Zi-Bin Zhang, Piao Zhang, Rong-Ping Zhang, Ting-Hua Wang
Neural stem cells (NSCs) are characterized by the ability of self‑renewal and capacity to proliferate and produce new nervous tissue. NSCs are capable of differentiating to three lineages of neural cells, including neurons, oligodendrocytes and astrocytes. Furthermore, hippocampal NSCs transplantation can improve the neurological deficits associated with expression of cytokines. Therefore, to compare the properties of NSCs of tree shrews and rats in vitro, NSCs from tree shrews (tsNSCs) and rats f(rNSCs) were isolated...
February 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29434962/electron-microscopic-analysis-of-different-cell-types-in-human-pancreatic-cancer-spheres
#9
Toshiyuki Ishiwata, Fumio Hasegawa, Masaki Michishita, Norihiko Sasaki, Naoshi Ishikawa, Kaiyo Takubo, Yoko Matsuda, Tomio Arai, Junko Aida
Cancer stem cells (CSCs), which are pluripotent and self-renewable, contribute to the initiation and metastasis of cancer, and are responsible for resistance to chemotherapy and radiation. Pancreatic ductal adenocarcinoma (PDAC) is an aggressive type of cancer that is associated with a high incidence of distant metastasis and recurrence. Sphere formation reveals cell proliferation under nonadherent conditions and is commonly used to identify CSCs; measurements of the number, area and volume of the spheres are used to estimate stemness of PDAC cells...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434302/retinal-progenitor-cells-release-extracellular-vesicles-containing-developmental-transcription-factors-microrna-and-membrane-proteins
#10
Jing Zhou, Alberto Benito-Martin, Jason Mighty, Lynne Chang, Shima Ghoroghi, Hao Wu, Madeline Wong, Sara Guariglia, Petr Baranov, Michael Young, Rajendra Gharbaran, Mark Emerson, Milica Tesic Mark, Henrik Molina, M Valeria Canto-Solar, Hector Peinado Selgas, Stephen Redenti
A range of cell types, including embryonic stem cells, neurons and astrocytes have been shown to release extracellular vesicles (EVs) containing molecular cargo. Across cell types, EVs facilitate transfer of mRNA, microRNA and proteins between cells. Here we describe the release kinetics and content of EVs from mouse retinal progenitor cells (mRPCs). Interestingly, mRPC derived EVs contain mRNA, miRNA and proteins associated with multipotency and retinal development. Transcripts enclosed in mRPC EVs, include the transcription factors Pax6, Hes1, and Sox2, a mitotic chromosome stabilizer Ki67, and the neural intermediate filaments Nestin and GFAP...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29428671/swertisin-ameliorates-diabetes-by-triggering-pancreatic-progenitors-for-islet-neogenesis-in-streptozotocin-treated-balb-c-mice
#11
Abhay Srivastava, Nidheesh Dadheech, Mitul Vakani, Sarita Gupta
In the present study, Swertisin's role in triggering resident pancreatic progenitors for islet neogenesis in Streptozotocin (STZ) diabetic mice was explored. STZ diabetic mice when treated with Swertisin demonstrated reversion to normoglycemia and significant elevation of fasting serum insulin levels. On screening the pancreatic tissue post Swertisin treatment in the STZ diabetic mice, we observed significant up-regulation of key transcription factors viz. Pdx1, Neurog3, MafA and Nkx6.1 required for islet neogenesis and beta cell homeostasis...
February 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29422311/merkel-cell-carcinoma-in-a-steer
#12
A P P Teh, U Z Izzati, N V Diep, T Hirai, R Yamaguchi
Merkel cell carcinoma is a rare and aggressive cutaneous neuroendocrine tumour reported only in man, dogs and cats. A 20-month-old Japanese black fattening steer was presented with necrotic protruding skin masses over the left thoracic area and a 20 × 25 cm subcutaneous mass in the left abdominal area. Microscopical evaluation of the masses revealed cords of small to medium-sized round tumour cells with marked anisocytosis and anisokaryosis and clear and vacuolated cytoplasm, which were separated by a delicate fibrovascular stroma and arranged in a trabecular and nested pattern...
January 2018: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/29416637/liver-x-receptors-agonist-promotes-differentiation-of-rat-bone-marrow-derived-mesenchymal-stem-cells-into-dopaminergic-neuron-like-cells
#13
Oumei Cheng, Xiaoyan Tian, Ying Luo, Shaoshan Mai, Yang Yang, Shengnan Kuang, Qi Chen, Jie Ma, Beibei Chen, Rong Li, Lu Yang, Huan Li, Congli Hu, Jiahua Zhang, Zhihao Chen, Yuke Li, Hui Xia, Ying Xu, Junqing Yang
Dopaminergic (DA) neurons derived from bone marrow derived mesenchymal stem cells (BMSCs) maybe a valuable source for cell replacement therapy in Parkinson disease. Recent studies showed that new functions of LXR and their ligands have been proposed to prevent PD in the adult nervous system. The present study was designed to observe the effect of liver X receptors (LXR) agonist on differentiation of rat BMSCs into DA neurons. Expressions of the neuronal markers (Tuj1 and Nestin), the specific marker of DA neurons (tyrosine hydroxylase, TH), LXR α and LXR β were measured by immunocytochemical assay and TH/Tuj1 positive cells were determined by quantitative cell count analyses...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29414342/dep-and-dbp-induce-cytotoxicity-in-mouse-embryonic-stem-cells-and-abnormally-enhance-neural-ectoderm-development
#14
Nuoya Yin, Shengxian Liang, Shaojun Liang, Bowen Hu, Renjun Yang, Qunfang Zhou, Guibin Jiang, Francesco Faiola
Diethyl phthalate (DEP) and dibutyl phthalate (DBP) are two typical small phthalate esters, extensively used in personal care and consumer products. Although previous studies have linked phthalate esters to several health issues, it is still unclear whether they can affects the early stages of embryonic development. In this study, we evaluated the early developmental neurotoxicity as well as the cytotoxicity of DEP and DBP, using mouse embryonic stem cells (mESCs). Our results showed that both DEP and DBP could decrease mESC viability in a dose-dependent manner...
February 1, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29411916/neural-androgen-receptors-affect-the-number-of-surviving-new-neurons-in-the-adult-dentate-gyrus-of-male-mice
#15
Ashlyn Swift-Gallant, Paula Duarte-Guterman, Dwayne K Hamson, Muna Ibrahim, D Ashley Monks, Liisa A M Galea
Adult hippocampal neurogenesis occurs in many mammalian species. In rats, the survival of new neurons within the hippocampus is modulated by androgen action via the androgen receptor (AR), however it is not known whether this holds true in mice. Furthermore, evidence is mixed as to whether androgens act in neural tissue or via peripheral non-neural targets to promote hippocampal neuron survival. We evaluated whether androgen action via AR underlies the survival of new neurons in mice, and asked whether increasing AR selectively in neural tissue would increase new neuron survival in the hippocampus...
February 7, 2018: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/29410383/concentrated-ambient-pm2-5-induced-inflammation-and-endothelial-dysfunction-in-a-murine-model-of-neural-ikk2-deficiency
#16
Minjie Chen, Xiaobo Qin, Lianglin Qiu, Sufang Chen, Huifen Zhou, Yanyi Xu, Ziying Hu, Yuhao Zhang, Qi Cao, Zhekang Ying
BACKGROUND: Exposure to ambient fine particulate matter (PM2.5) is associated with cardiovascular mortality, but underlying pathophysiologic mechanisms are not fully understood. Hypothalamic inflammation, characterized by the activation of Inhibitor kappaB kinase 2/Nuclear factor kappaB (IKK2/NF-κB) signaling pathway, may play an important role in the pathogenesis of cardiovascular diseases. We recently demonstrated that hypothalamic inflammation is increased in mice exposed to concentrated ambient PM2...
February 5, 2018: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29402326/treatment-of-gingival-defects-with-gingival-mesenchymal-stem-cells-derived-from-human-fetal-gingival-tissue-in-a-rat-model
#17
Jing Li, Shi-Qing Xu, Kai Zhang, Wen-Jian Zhang, Hong-Lin Liu, Zhen Xu, Hong Li, Jin-Ning Lou, Li-Hong Ge, Bao-Hua Xu
BACKGROUND: The study aimed to evaluate the efficacy and safety of gingival mesenchymal stem cells (GMSCs) from human fetal gingival tissue used for treating gingival defects in a rat model. METHODS: GMSCs were isolated from human fetal gingival tissue and identified by flow cytometry for nestin, Oct4, vimentin, NANOG, CD105, and CD90. The immunogenicity of GMSCs was analyzed by mixed lymphocyte reactions; the tumorigenicity of GMSCs was evaluated by xenotransplanting into nude mice...
February 5, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29399816/characterization-and-virus-susceptibility-of-a-continuous-cell-line-derived-from-the-brain-of-aequidens-rivulatus-g%C3%A3-nther
#18
S W Yeh, Y H Cheng, F N Nan, C M Wen
Cell cultures derived from the brain tissues of Aequidens rivulatus (Günther) have been characterized previously. In this study, a continuous cell line ARB8 was further established, and its growth characteristics, transcription and susceptibility to fish viruses-including chum salmon reovirus (CSV), marbled eel infectious pancreative necrosis virus (MEIPNV), grouper nervous necrosis virus (GNNV), giant seaperch iridovirus (GSIV), red seabream iridovirus (RSIV), koi herpesvirus (KHV), herpesvirus anguilla (HVA) and marbled eel polyoma-like virus (MEPyV)-were examined...
February 5, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29384270/cognitive-deficits-and-increases-in-creatine-precursors-in-a-brain-specific-knockout-of-the-creatine-transporter-gene-slc6a8
#19
Kenea C Udobi, Amanda N Kokenge, Emily R Hautman, Gabriela Ullio, Julie Coene, Michael T Williams, Charles V Vorhees, Aloïse Mabondzo, Matthew R Skelton
Creatine transporter (CrT; SLC6A8) deficiency (CTD) is an X-linked disorder characterized by severe cognitive deficits, impairments in language, and an absence of brain creatine (Cr). In a previous study, we generated floxed Slc6a8 (Slc6a8flox ) mice to create ubiquitous Slc6a8 knockout (Slc6a8-/y ) mice. Slc6a8-/y mice lacked whole body Cr and exhibited cognitive deficits. While Slc6a8-/y mice have a similar biochemical phenotype to CTD patients, they also showed a reduction in size and reductions in swim speed that may have contributed to the observed deficits...
January 31, 2018: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/29380193/the-emu-oil-emulsified-in-egg-lecithin-and-butylated-hydroxytoluene-enhanced-the-proliferation-stemness-gene-expression-and-in-vitro-wound-healing-of-adipose-derived-stem-cells
#20
Khatereh Saei Arezoumand, Effat Alizadeh, Mohammad Esmaeillou, Maryam Ghasemi, Shahriar Alipour, Younes Pilehvar-Soltanahmadi, Nosratollah Zarghami
In recent decades, mesenchymal stem cells originated from adipose tissue (adipose-derived stem cells, ASCs) have gained increased attention for production of cell-based therapeutics. Emu oil as a natural compound showed antioxidant effects in previous studies. The goal of this study was to investigate the effect of crude emu oil on the proliferation, cell cycle progression, stemness genes expression, and in vitro wound healing potential of ASCs. An emulsion of emu oil was prepared using egg lecithin and butylated hydroxytoluene to improve bioavailability and solubility of emu oil in the expansion medium...
January 29, 2018: In Vitro Cellular & Developmental Biology. Animal
keyword
keyword
47025
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"