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https://www.readbyqxmd.com/read/27913752/expression-and-bioactivity-of-human-alpha-fetoprotein-in-a-bac-to-bac-system
#1
Bo Lin, Kun Liu, Wenting Wang, Wei Li, Xu Dong, Yi Chen, Yan Lu, Junli Guo, Mingyue Zhu, Mengsen Li
Alpha-fetoprotein (AFP) is an early serum growth factor in fetal embryonic development and hepatic oncogenesis. A growing number of investigations of AFP as a tumor-specific biomarker have concluded that AFP is an important target for cancer treatment. AFP also plays an immunomodulatory role in the treatment of several autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, myasthenia gravis, and thyroiditis. In an effort to support biochemical screening and drug design and discovery, we attempted to express and purify human AFP in a Bac-to-Bac system...
December 2, 2016: Bioscience Reports
https://www.readbyqxmd.com/read/27908418/lead-pb-exposure-promotes-diabetes-in-obese-rodents
#2
Jannifer B Tyrrell, Samar Hafida, Paul Stemmer, Angie Adhami, Todd Leff
BACKGROUND: Pb (lead) exposure occurs at elevated frequency in urban inner city populations that also have high rates of obesity and diabetes. OBJECTIVES: To determine if Pb can promote the development of diabetes in a setting of obesity, we examined the effect of Pb exposure on glucose metabolism in a rodent model of obesity. METHODS: Adult female ZDF rats were exposed to Pb in drinking water for 24 weeks. Fasting blood glucose, insulin, and glucose tolerance were measured at regular intervals...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27907881/effect-of-toad-skin-extracts-on-the-pain-behavior-of-cancer-model-mice-and-its-peripheral-mechanism-of-action
#3
Tao Chen, Shen-Jun Yuan, Xue-Qin Yu, Liang-Bo Jiao, Wei Hu, Wang-Long Chen, Bo Xie
The changes in thermal and mechanical hyperalgesia in paw cancer pain model mice and the action mechanism of toad skin extracts (TSE) was investigated. Eighty female mice were subcutaneously injected with saline or inoculated with H22 hepatoma cells in the right hind paw and administration with saline, vehicle, morphine and TSE. The pain behavior was recorded before treatment and at 0.5, 1.0, 1.5, 3 and 6h after initial administration, and thereafter on the 2nd, 4th, 6th, and 8th day after administration. On the last day, samples were collected after the euthanasia for the detection of β-END, CRF, IL-1β, POMC, μ-OR, CD3+, CD8+ and CD4+ in sera and the tumor tissues...
November 28, 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27907035/exendin-4-inhibits-hepatic-lipogenesis-by-increasing-%C3%AE-catenin-signaling
#4
Mi Hae Seo, Jinmi Lee, Seok-Woo Hong, Eun-Jung Rhee, Se Eun Park, Cheol Young Park, Ki Won Oh, Sung Woo Park, Won-Young Lee
The aim of this study is to investigate whether the beneficial effect of exendin-4 on hepatic steatosis is mediated by β-catenin signaling. After the HepG2 human hepatoma cells were treated with PA for 24 hours, total triglycerides levels were increased in a dose-dependent manner, and the expression levels of perilipin family members were upregulated in cells treated with 400 μM PA. For our in vitro model of hepatic steatosis, HepG2 cells were treated with 400 μM palmitic acid (PA) in the presence or absence of 100 nM exendin-4 for 24 hours...
2016: PloS One
https://www.readbyqxmd.com/read/27902355/the-use-of-human-umbilical-cord-blood-serum-is-beneficial-for-the-continuous-production-of-hepatitis-c-virus
#5
Xiao-Yang Jiao, Rineke H G Steenbergen, D Lorne Tyrrell
In this study we investigated if human umbilical cord blood serum (CBS) is a suitable replacement for fetal bovine serum (FBS) in cultures of human hepatoma cell line Huh7.5, particularly regarding its capacity to maintain high growth rates, differentiation status and its ability to support robust HCV infection. Generally, CBS cultured Huh7.5 cells remained comparable to FBS cultured cells, and proliferated equally well. Albumin secretion, a hepatocyte differentiation marker, had increased 8x in CBS, however, most other hepatocyte markers we tested had not changed...
October 24, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27900002/therapeutic-targeting-of-liver-cancer-with-a-recombinant-dna-vaccine-containing-the-hemagglutinin-neuraminidase-gene-of-newcastle-disease-virus-via-apoptotic-dependent-pathways
#6
Li-Gang Chen, Yuan-Sheng Liu, Tang-Hui Zheng, Xu Chen, Ping Li, Chuan-Xing Xiao, Jian-Lin Ren
A total of ~38.6 million mortalities occur due to liver cancer annually, worldwide. Although a variety of therapeutic methods are available, the efficacy of treatment at present is extremely limited due to an increased risk of malignancy and inherently poor prognosis of liver cancer. Gene therapy is considered a promising option, and has shown notable potential for the comprehensive therapy of liver cancer, in keeping with advances that have been made in the development of cancer molecular biology. The present study aimed to investigate the synergistic effects of the abilities of the hemagglutinin neuraminidase protein of Newcastle disease virus (NDV), the pro-apoptotic factor apoptin from chicken anaemia virus, and the interferon-γ inducer interleukin-18 (IL-18) in antagonizing liver cancer...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27899290/didymin-induces-apoptosis-through-mitochondrial-dysfunction-and-up-regulation-of-rkip-in-human-hepatoma-cells
#7
Jinbin Wei, Quanfang Huang, Facheng Bai, Jun Lin, Jinlan Nie, Shengjuan Lu, Chunyuang Lu, Renbin Huang, Zhongpeng Lu, Xing Lin
In the present study, a flavonoid was isolated from Origanum vulgare and identified as didymin. The effect and mechanism of O. vulgare didymin (OVD) on human HepG2 liver carcinoma cell was then assessed. Our results showed that OVD strongly inhibited the viability, clonogenicity and migration of HepG2 cells. OVD significantly induced apoptosis and induced cell cycle arrest at G2/M phase by regulating cyclin B1, cyclin D1 and CDK4. The anti-proliferative and pro-apoptotic effects were associated with changes in the Bcl-2/Bax ratio and induction of caspase-mediated apoptosis...
November 26, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27899122/endocytosed-nanoparticles-hold-endosomes-and-stimulate-binucleated-cells-formation
#8
Lin Xia, Weihong Gu, Mingyi Zhang, Ya-Nan Chang, Kui Chen, Xue Bai, Lai Yu, Juan Li, Shan Li, Gengmei Xing
BACKGROUND: Nanotechnology developed rapidly in cellular diagnosis and treatment, the endocytic system was an important pathway for targeting cell. In the research of developing macrophages as drug carriers or important therapeutic targets, an interesting phenomenon, internalized nanoparticles induced to form binucleated macrophages, was found although the particles dose did not cause obvious cytotoxicity. RESULTS: Under 25 μg/ml, internalized 30 nm polystyrene beads(30 nm Ps nanoparticles) induced the formation of binucleated macrophages when they entered into endosomes via the endocytic pathway...
November 29, 2016: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/27896668/target-specific-delivery-of-sirna-into-hepatoma-cells-cytoplasm-by-bifunctional-carrier-peptide
#9
Xiaoxuan Liu, Lin Zhu, Jingjing Ma, Xinxiao Qiao, Dunwan Zhu, Lanxia Liu, Xigang Leng
RNA interference (RNAi) is among the most potential approach for the therapy of hepatocellular carcinoma and the major barrier hindering siRNA therapeutics is the low efficiency of delivery to the desired cells. The current study aimed at developing a novel peptide for more efficient hepatoma targeted siRNA delivery, by combining luteinizing hormone-releasing hormone with hepatoma targeting specificity and MPG(△NLS) with cytoplasm-delivery tendency. The developed bifunctional peptide LHRH-MPG(△NLS) and siRNA were mixed together and resulted in LHRH-MPG(△NLS)/siRNA polyplexes through self-assembly...
November 28, 2016: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/27896567/foxo1-and-lxr%C3%AE-downregulate-the-apolipoprotein-a-i-gene-expression-during-hydrogen-peroxide-induced-oxidative-stress-in-hepg2-cells
#10
Vladimir S Shavva, Alexandra M Bogomolova, Artemy A Nikitin, Ella B Dizhe, Galina N Oleinikova, Ivan A Lapikov, Dmitry A Tanyanskiy, Andrej P Perevozchikov, Sergey V Orlov
Reactive oxygen species damage various cell components including DNA, proteins, and lipids, and these impairments could be a reason for severe human diseases including atherosclerosis. Forkhead box O1 (FOXO1), an important metabolic transcription factor, upregulates antioxidant and proapoptotic genes during oxidative stress. Apolipoprotein A-I (ApoA-I) forms high density lipoprotein (HDL) particles that are responsible for cholesterol transfer from peripheral tissues to liver for removal in bile in vertebrates...
November 28, 2016: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/27895771/co-culture-of-hepatoma-cells-with-hepatocytic-precursor-stem-like-cells-inhibits-tumor-cell-growth-and-invasion-by-downregulating-akt-nf-%C3%AE%C2%BAb-expression
#11
Cheng-Jun Sui, Miao Xu, Wei-Qing Li, Jia-Mei Yang, Hong-Zhu Yan, Hui-Min Liu, Chun-Yan Xia, Hong-Yu Yu
Hepatocytic stem cells (HSCs) have inhibitory effects on hepatocarcinoma cells. The present study investigated the effects of HSC activity in hepatocarcinoma cells in vitro. A Transwell co-culture system of hepatocytic precursor (stem-like) WB-F344 cells and hepatoma CBRH-7919 cells was used to assess HSC activity in metastasized hepatoma cells in vitro. Nude mouse xenografts were used to assess HSC activity in vivo. Co-culture of hepatoma CBRH-7919 cells with WB-F344 cells suppressed the growth and colony formation, tumor cell migration and invasion capacity of CBRH-7919 cells...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895723/cisplatin-induced-regulation-of-signal-transduction-pathways-and-transcription-factors-in-p53-mutated-subclone-variants-of-hepatoma-cells-potential-application-for-therapeutic-targeting
#12
Jinn-Rung Kuo, Hung-Sheng Shang, Chun-Te Ho, Kun-Goung Lai, Tsan-Zon Liu, Yin-Ju Chen, Jeng-Fong Chiou
Cisplatin is commonly recognized as a DNA-damaging drug; however, its versatile antitumor effects have been demonstrated to extend beyond this narrow functional attribute. The present study determined how cisplatin regulates alternative pathways and transcription factors to exert its additional antitumor actions. Cisplatin was observed to be able to trigger an endoplasmic reticulum stress response through aggravated nitrosative stress coupled to perturbed mitochondrial calcium (Ca(2+)) homeostasis, which substantially downregulated glucose-regulated protein (GRP) 78 expression by suppressing the cleavage of activating transcription factor (ATF) 6α (90 kDa) to its active 50 kDa subunit...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27894814/tanshinone-iia-induced-cell-death-via-mir30b-p53-ptpn11-shp2-signaling-pathway-in-human-hepatocellular-carcinoma-cells
#13
Xuanqi Ren, Cui Wang, Binbin Xie, Linfeng Hu, Hui Chai, Lei Ding, Lihua Tang, Yongliang Xia, Xiaobing Dou
Tanshinone IIA, a multi-pharmaceutical compound from traditional Chinese herb, has been reported to have anti-hepatocarcinomic (HCC) properties through cell death induction. Apart from the typical p53-dependent pathway, mechanisms of the anti-carcinogenic role of Tanshinone remain scarce. In an effort to explore the mechanism behind Tanshinone IIA, we detected the upstream of the p53 and the potential novel pathway. Tanshinone IIA dose-dependently initiated HepG2 cell apoptosis and cell cycle arrest at the G1 checkpoint...
November 25, 2016: European Journal of Pharmacology
https://www.readbyqxmd.com/read/27894242/indazolo-3-2-b-quinazolinones-attack-hepatocellular-carcinoma-hep3b-cells-by-inducing-mitochondrial-dependent-apoptosis-and-inhibition-of-nrf2-are-signaling-pathway
#14
Y Zhang, R Qiao, D He, Z Zhao, S Yang, H Zou, X Zhang, M Wu, J Chen, P Chen
Hepatocellular carcinoma (HCC) is the second leading cause of cancer death worldwide. Genotoxic stress resistance in patients often contributes to poor clinical outcomes, and is intensively associated to the upregulation of Nrf2/ARE signaling pathway. In this study, we examined the connection between the anti-cancer activity of two novel indazolo[3,2-b]quinazolinone (IQ) derivatives, IQ-7 and IQ-12, and their effect on the Nrf2/ARE signaling pathway. The two compounds were shown to induce apoptosis and inhibit the Nrf2/ARE signaling pathway in Hep3B cells (human hepatoma cell line)...
November 28, 2016: Current Molecular Medicine
https://www.readbyqxmd.com/read/27886437/the-microrna-99-family-modulates-hepatitis-b-virus-replication-by-promoting-igf-1r-pi3k-akt-mtor-ulk1-signaling-induced-autophagy
#15
Yong Lin, Wanyu Deng, Jinke Pang, Thekla Kemper, Jing Hu, Jian Yin, Jiming Zhang, Mengji Lu
MicroRNAs (miRNAs) are small highly conserved noncoding RNAs that are widely expressed in multicellular organisms and participate in the regulation of various cellular processes including autophagy and viral replication. Evidently, miRNAs are able to modulate host gene expression and thereby inhibit or enhance hepatitis B virus (HBV) replication. The miR-99 family members are highly expressed in the liver. Interestingly, the plasma levels of miR-99 family in the peripheral blood correspond with HBV DNA loads...
November 25, 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27881334/-over-expression-of-receptor-interacting-protein-140-in-tumor-associated-macrophages-suppresses-invasion-and-proliferation-of-hepatoma-cells-in-vitro
#16
Zhu-Jun Yi, Yun Zhou, Wen-Feng Zhang, Hao Chen, Wei Zhao, Zuo-Jin Liu, Jian-Ping Gong
OBJECTIVE: To investigate the role of receptor-interacting protein 140 (RIP140) in tumor-associated macrophages (TAMs) in the invasion and proliferation of hepatoma cells in vitro. METHODS: Western blotting, qRT-PCR and flow cytometry were performed to examine the effects of lentivirus-mediated RIP140 over-expression in mouse peritoneal macrophages (PMs). Western blotting, qRT-PCR and immunofluorescence staining were used to detect the expression of RIP140 in TAMs following stimulation of the PMs with hepatocellular carcinoma conditioned medium (HCM) for 24 h...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27879394/selective-control-of-up-regulated-and-down-regulated-genes-by-temporal-patterns-and-doses-of-insulin
#17
Takanori Sano, Kentaro Kawata, Satoshi Ohno, Katsuyuki Yugi, Hiroaki Kakuda, Hiroyuki Kubota, Shinsuke Uda, Masashi Fujii, Katsuyuki Kunida, Daisuke Hoshino, Atsushi Hatano, Yuki Ito, Miharu Sato, Yutaka Suzuki, Shinya Kuroda
Secretion of insulin transiently increases after eating, resulting in a high circulating concentration. Fasting limits insulin secretion, resulting in a low concentration of insulin in the circulation. We analyzed transcriptional responses to different temporal patterns and doses of insulin in the hepatoma FAO cells and identified 13 up-regulated and 16 down-regulated insulin-responsive genes (IRGs). The up-regulated IRGs responded more rapidly than did the down-regulated IRGs to transient stepwise or pulsatile increases in insulin concentration, whereas the down-regulated IRGs were repressed at lower concentrations of insulin than those required to stimulate the up-regulated IRGs...
November 22, 2016: Science Signaling
https://www.readbyqxmd.com/read/27873035/dmfc-3-5-dimethyl-7-h-furo-3-2-g-chromen-7-one-regulates-bim-to-trigger-bax-and-bak-activation-to-suppress-drug-resistant-human-hepatoma
#18
Jun Xiang, Zhe Wang, Qianqian Liu, Xia Li, Jianguo Sun, Kwok-Pui Fung, Feiyan Liu
3,5-Dimethyl-(7)H-furo[3,2-g]chromen-7-one (DMFC) is a coumarin derivative with anti-cancer activity against human hepatoma cells, but the mechanisms underlying DMFC function in cancer suppression is unknown. In this study, we aimed at elucidating the molecular mechanisms underlying DMFC anti-cancer activity and determining whether DMFC is effective in suppression of drug-resistant human hepatocellular carcinoma. We show here that DMFC effectively suppresses both the parent and the multidrug-resistant hepatoma cell growth in vitro and DMFC suppresses hepatoma cell growth at least in part through inducing tumor cell apoptosis...
November 21, 2016: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27872984/cinnamic-acid-derivatives-enhance-the-efficacy-of-transarterial-embolization-in-a-rat-model-of-hepatocellular-carcinoma
#19
Luke R Wilkins, David L Brautigan, Hanping Wu, Hooman Yarmohammadi, Ewa Kubicka, Vlad Serbulea, Norbert Leitinger, Wendy Liu, John R Haaga
INTRODUCTION: We hypothesize that the combination of transarterial embolization (TAE) plus inhibition of lactate export will limit anaerobic metabolism and reduce tumor survival compared to TAE alone. The purpose of this study was to test this hypothesis in a rat model of hepatocellular carcinoma (HCC). METHODS: Rat N1-S1 hepatoma cells were assayed in vitro using the Seahorse XF analyzer to measure extracellular acidification (lactate excretion) comparing effects of the addition of caffeic acid (CA) or ferulic acid (FA) or UK-5099 with control...
November 21, 2016: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/27860468/porphyrin-based-carbon-dots-for-photodynamic-therapy-of-hepatoma
#20
Yang Li, Xiaohua Zheng, Xiaoyu Zhang, Shi Liu, Qing Pei, Min Zheng, Zhigang Xie
Porphyrin-containing carbon dots (CDs) possess ultrasmall size, excellent water solubility, and photostability. These CDs can effectively generate cytotoxic singlet oxygen upon irradiation, and induce the cell apoptosis. Photodynamic ability of CDs inhibits the growth of hepatoma. This work not only sheds light on developing functional carbon dots, but also highlights the importance of special-structure precursor molecules in synthesizing functional CDs.
November 18, 2016: Advanced Healthcare Materials
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