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https://www.readbyqxmd.com/read/28922602/cucurbit-7-uril-induced-formation-of-fret-enabled-uni-lamellar-lipid-vesicles
#1
Shilpi Kushwaha, Arunava Maity, Monalisa Gangopadhyay, Sapna Ravindranathan, Pattuparambil R Rajamohanan, Amitava Das
A unique fluorescence resonance energy transfer (FRET) process is found to be operational in a unilamellar lipid self-assembly in the aqueous phase. A newly synthesized naphthyl based long chain lipid derivative [N-(naphthalene-1-ylmethyl)tetradecane-1-ammonium chloride, 14NA+] forms various self-assembled architectures in the aqueous phase. Controlled changes in lipid concentration lead to a transition of the self-assemblies from micelles to vesicles to rods. In the presence of cucurbit[7]uril (CB7), 14NA+ forms a host-guest [2]pseudorotaxane complex (CB7∋14NA+) and secondary interactions lead to the formation of a lipid bilayer with hydrophobic pockets situated in between the layers...
September 18, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28922156/soluble-oligomers-require-a-ganglioside-to-trigger-neuronal-calcium-overload
#2
Roberta Cascella, Elisa Evangelisti, Alessandra Bigi, Matteo Becatti, Claudia Fiorillo, Massimo Stefani, Fabrizio Chiti, Cristina Cecchi
An altered distribution of membrane gangliosides (GM), including GM1, has recently been reported in the brains of Alzheimer's disease (AD) patients. Moreover, amyloid-positive synaptosomes obtained from AD brains were found to contain high-density GM1 clusters, suggesting a pathological significance of GM1 increase at presynaptic neuritic terminals in AD. Here, we show that membrane GM1 specifically recruits small soluble oligomers of the 42-residue form of amyloid-β peptide (Aβ42), with intracellular flux of Ca2+ ions in primary rat hippocampal neurons and in human neuroblastoma cells...
September 8, 2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28920931/single-molecule-fret-reveals-the-energy-landscape-of-the-full-length-sam-i-riboswitch
#3
Christoph Manz, Andrei Yu Kobitski, Ayan Samanta, Bettina G Keller, Andres Jäschke, G Ulrich Nienhaus
S-adenosyl-L-methionine (SAM) ligand binding induces major structural changes in SAM-I riboswitches, through which gene expression is regulated via transcription termination. Little is known about the conformations and motions governing the function of the full-length Bacillus subtilis yitJ SAM-I riboswitch. Therefore, we have explored its conformational energy landscape as a function of Mg(2+) and SAM ligand concentrations using single-molecule Förster resonance energy transfer (smFRET) microscopy and hidden Markov modeling analysis...
September 18, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28919480/cholesterol-modulates-the-cellular-localization-of-orai1-channels-and-its-disposition-among-membrane-domains
#4
A Bohórquez-Hernández, Enrico Gratton, Jonathan Pacheco, Alexander Asanov, Luis Vaca
Store Operated Calcium Entry (SOCE) is one of the most important mechanisms for calcium mobilization in to the cell. Two main proteins sustain SOCE: STIM1 that acts as the calcium sensor in the endoplasmic reticulum (ER) and Orai1 responsible for calcium influx upon depletion of ER. There are many studies indicating that SOCE is modulated by the cholesterol content of the plasma membrane (PM). However, a myriad of questions remain unanswered concerning the precise molecular mechanism by which cholesterol modulates SOCE...
September 12, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28918008/a-finite-element-study-on-the-mechanical-response-of-the-head-neck-interface-of-hip-implants-under-realistic-forces-and-moments-of-daily-activities-part-2
#5
Khosro Fallahnezhad, Hamidreza Farhoudi, Reza H Oskouei, Mark Taylor
A finite element model was developed to investigate the effect of loading regimes caused by various daily activities on the mechanical behaviour of the head-neck taper junction in modular hip replacements. The activities included stair up, stair down, sit to stand, stand to sit, one leg standing and knee bending. To present the real mechanical environment of the junction, in addition to the force components, the frictional moments produced by the frictional sliding of the head and cup were applied to a CoCr/CoCr junction having a 12/14 taper with a proximal mismatch angle of 0...
September 7, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28917774/fret-on-lateral-flow-test-strip-to-enhance-sensitivity-for-detecting-cancer-biomarker
#6
Jidong Wang, Fengjing Cao, Songliang He, Yong Xia, Xinyu Liu, Wenxiao Jiang, Yangyang Yu, Huisheng Zhang, Wenwen Chen
Fluorescence resonance energy transfer (FRET) between fluorescein isothiocyanate (FITC) and gold nanoparticles (Au NPs) is introduced in the lateral flow strip to detect cancer biomarker CEA with the color and fluorescence dual-readout. Anti-CEA monoclonal antibody coated Au NPs were on the conjugate pad and FITC labelled antibody (FITC-Ab) for CEA was coated on the test line. All the reagents were general in the lateral flow strip or commercially available and no new materials or technique were involved, which make our proposal a more universal method and easier to operate...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28916818/identification-of-4-phenylquinolin-2-1h-one-as-a-specific-allosteric-inhibitor-of-akt
#7
Bill X Huang, Kenny Newcomer, Karl Kevala, Elena Barnaeva, Wei Zheng, Xin Hu, Samarjit Patnaik, Noel Southall, Juan Marugan, Marc Ferrer, Hee-Yong Kim
Akt plays a major role in tumorigenesis and the development of specific Akt inhibitors as effective cancer therapeutics has been challenging. Here, we report the identification of a highly specific allosteric inhibitor of Akt through a FRET-based high-throughput screening, and characterization of its inhibitory mechanism. Out of 373,868 compounds screened, 4-phenylquinolin-2(1H)-one specifically decreased Akt phosphorylation at both T308 and S473, and inhibited Akt kinase activity (IC50 = 6 µM) and downstream signaling...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28915384/supramolecular-nano-sniffers-for-ultrasensitive-detection-of-formaldehyde
#8
Uchangi Satyaprasad Akshath, Praveena Bhatt
Supramolecular nanoparticle hybrids for biosensing of analytes have been a major focus due to their tunable optical and surface properties. Quantum dots-Gold nanoparticle (QDs-GNP) based FRET probes involving turn on/off principles have gained immense interest due to their specificity and sensitivity. Recent focus is on applying these supramolecular hybrids for enzyme operated biosensors that can specifically turn-on fluorescence induced by co-factor or product formed from enzymatic reaction. The present study focuses on locking and unlocking the interaction between QD-GNP pair leading to differential fluorescent properties...
September 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28914606/nanoscale-architecture-of-the-schizosaccharomyces-pombe-contractile-ring
#9
Nathan A McDonald, Abigail L Lind, Sarah E Smith, Rong Li, Kathleen Gould
The contractile ring is a complex molecular apparatus important for dividing many eukaryotic cells. Despite knowledge of its composition, the molecular architecture of the ring is not known. Here we applied super-resolution microscopy and FRET to determine the nanoscale spatial organization of Schizosaccharomyces pombe contractile ring components relative to the plasma membrane. As in other membrane-tethered actin structures, contractile ring proteins are stratified relative to the membrane. The lowest layer (0-80 nm) contains membrane-binding scaffolds, formin, and the myosin-II tail...
September 15, 2017: ELife
https://www.readbyqxmd.com/read/28914010/fret-based-method-for-evaluation-of-the-efficiency-of-reversible-and-irreversible-sonoporation
#10
Paulius Ruzgys, Mindaugas Tamošiūnas, Vanesa Lukinsone, Saulius Šatkauskas
It is widely known that not all of the treated cells survive after introduction of exogenous molecules via any physical method. Therefore, it is important to develop methods that would allow simultaneous evaluation of both molecular delivery efficiency and cell viability. This study presents Förster resonance energy transfer (FRET)-based method that allows molecular transfer and cell viability evaluation in a single measurement by employing two common fluorescent dyes, namely, ethidium bromide and trypan blue...
September 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28892380/gne-781-a-highly-advanced-potent-and-selective-bromodomain-inhibitor-of-cyclic-adenosine-monophosphate-response-element-binding-protein-binding-protein-cbp
#11
F Anthony Romero, Jeremy M Murray, Kwong Wah Lai, Vickie Tsui, Brian K Albrecht, Le An, Maureen H Beresini, Gladys de Leon Boenig, Sarah M Bronner, Emily W Chan, Kevin X Chen, Zhongguo Chen, Edna F Choo, Kyle Clagg, Kevin Clark, Terry D Crawford, Patrick Cyr, Denise De Almeida Nagata, Karen E Gascoigne, Jane L Grogan, Georgia Hatzivassiliou, Wei Huang, Thomas L Hunsaker, Susan Kaufman, Stefan G Koenig, Ruina Li, Yingjie Li, Xiaorong Liang, Jiangpeng Liao, Wenfeng Liu, Justin Q Ly, Jonathan Maher, Colin Masui, Mark Merchant, Yingqing Ran, Alexander M Taylor, John S Wai, Fei Wang, Xiaocang Wei, Dong Yu, Bing-Yan Zhu, Xiaoyu Zhu, Steven R Magnuson
Inhibition of the bromodomain of the transcriptional regulator CBP/P300 is an especially interesting new therapeutic approach in oncology. We recently disclosed in vivo chemical tool 1 (GNE-272) for the bromodomain of CBP that was moderately potent and selective over BRD4(1). In pursuit of a more potent and selective CBP inhibitor we used structure-based design. Constraining the aniline of 1 into a tetrahydroquinoline motif maintained potency and increased selectivity 2-fold. Structure-activity relationship studies coupled with further structure-based design targeting the LPF shelf, BC loop and KAc regions allowed us to significantly increase potency and selectivity, resulting in the identification of non-CNS penetrant 19 (GNE-781; TR-FRET IC50 = 0...
September 11, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28888786/microwave-assisted-synthesis-of-ruthenium-ii-complexes-with-alkynes-as-potential-inhibitor-by-selectively-recognizing-c-myc-g-quadruplex-dna
#12
Shuangyan Zhang, Qiong Wu, Hao Zhang, Qi Wang, Xicheng Wang, Wenjie Mei, Xiaohui Wu, Wenjie Zheng
Herein, two polypyridyl ruthenium(II) complexes with alkynes, [Ru(bpy)2L](ClO4)2 (L=p-TEPIP (1) and p-BEPIP (2); bpy=2,2'-bipyridine; p-TEPIP=2-(4-trimethylsilylpropargyl)-1H-imidazo[4,5f][1,10]phenanthroline; p-BEPIP=2-(4-phenyacetylenephenyl)-1H-imidazo[4,5f][1,10]phenanthroline) have been successfully achieved in yields of 32%-89% by a Sonogashira coupling reaction under microwave irradiation. We studied these complexes as potential stabilizers of c-myc G-quadruplex DNA. Observations revealed that both complexes could selectively bind to and stabilize c-myc G-quadruplex DNA with a constant of approximately 1...
August 30, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28887488/the-chemical-form-of-metal-species-released-from-corroded-taper-junctions-of-hip-implants-synchrotron-analysis-of-patient-tissue
#13
Anna Di Laura, Paul D Quinn, Vasiliki C Panagiotopoulou, Harry S Hothi, Johann Henckel, Jonathan J Powell, Fitim Berisha, Fernanda Amary, J Fred W Mosselmans, John A Skinner, Alister J Hart
The mechanisms of metal release from the articulation at the head cup bearing and the tapered junctions of orthopaedic hip implants are known to differ and the debris generated varies in size, shape and volume. Significantly less metal is lost from the taper junction between Cobalt-Chromium-Molybdenum (CoCrMo) and Titanium (Ti) components (fretting-corrosion dominant mechanism), when compared to the CoCrMo bearing surfaces (wear-corrosion dominant mechanism). Corrosion particles from the taper junction can lead to Adverse Reactions to Metal Debris (ARMD) similar to those seen with CoCrMo bearings...
September 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28887451/evolution-of-protein-coupled-rna-dynamics-during-hierarchical-assembly-of-ribosomal-complexes
#14
Sanjaya C Abeysirigunawardena, Hajin Kim, Jonathan Lai, Kaushik Ragunathan, Mollie C Rappé, Zaida Luthey-Schulten, Taekjip Ha, Sarah A Woodson
Assembly of 30S ribosomes involves the hierarchical addition of ribosomal proteins that progressively stabilize the folded 16S rRNA. Here, we use three-color single molecule FRET to show how combinations of ribosomal proteins uS4, uS17 and bS20 in the 16S 5' domain enable the recruitment of protein bS16, the next protein to join the complex. Analysis of real-time bS16 binding events shows that bS16 binds both native and non-native forms of the rRNA. The native rRNA conformation is increasingly favored after bS16 binds, explaining how bS16 drives later steps of 30S assembly...
September 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28886457/competition-derived-fret-switching-cationic-conjugated-polymer-ir-iii-complex-probe-for-thrombin-detection
#15
Chunnuan Du, Yufang Hu, Qingqing Zhang, Zhiyong Guo, Guoping Ge, Sui Wang, Chunyang Zhai, Mingshan Zhu
A novel, label-free and convenient strategy for thrombin assay has been developed based on the fluorescence resonance energy transfer (FRET) from a cationic conjugated polymer (CCP) to Ir(III) complex. The energy donor (CCP) and acceptor (Ir(III) complex) were taken into close proximity through π-π stacking interaction and electrostatic interaction, leading to the occurrence of FRET. However, the introduction of the thrombin aptamer upset the status and blocked the FRET process, but afterwards the reappearance of FRET phenomenon was confirmed by the special binding interaction between aptamer and thrombin, thus achieving the quantitative detection of thrombin...
August 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28885735/the-seating-mechanics-of-head-neck-modular-tapers-in-vitro-load-displacement-measurements-moisture-and-rate-effects
#16
Eric S Ouellette, Aarti A Shenoy, Jeremy L Gilbert
The mechanically-assisted crevice corrosion performance of head-neck modular tapers is a significant concern in orthopedic biomaterials. Fretting crevice corrosion processes in modular tapers are thought to be influenced by a wide array of factors including seating mechanics of the junction, hence there is a need for in vitro test methods that can assess their performance. This study presented a test method to directly measure the load-displacement seating mechanics of modular tapers and used this method to compare the seating mechanics for different tapers, moisture, seating loads and seating rates...
September 8, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28884489/voltage-and-wear-debris-from-ti-6al-4v-interact-to-affect-cell-viability-during-in-vitro-fretting-corrosion
#17
Tiantian Hui, Gregory W Kubacki, Jeremy L Gilbert
Fretting corrosion (or mechanically assisted corrosion, MAC) is a major corrosion mechanism in modular orthopedic implants. There is a complex interplay between fretting corrosion and the surrounding biological environment that includes particle generation and electrochemical potential excursions and currents. The goal of this work is to directly investigate the effects of fretting on cells in vitro. Using an in vitro fretting device, MC3T3 preosteoblasts were cultured onto Ti-6Al-4V disks adjacent to the fretting site...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28883449/graphene-quantum-dot-solid-sheets-strong-blue-light-emitting-photocurrent-producing-band-gap-opened-nanostructures
#18
Ganapathi Bharathi, Devaraj Nataraj, Sellan Premkumar, Murugaiyan Sowmiya, Kittusamy Senthilkumar, T Daniel Thangadurai, Oleg Yu Khyzhun, Mukul Gupta, Deodatta Phase, Nirmalendu Patra, Shambhu Nath Jha, Dibyendu Bhattacharyya
Graphene has been studied intensively in opto-electronics, and its transport properties are well established. However, efforts to induce intrinsic optical properties are still in progress. Herein, we report the production of micron-sized sheets by interconnecting graphene quantum dots (GQDs), which are termed 'GQD solid sheets', with intrinsic absorption and emission properties. Since a GQD solid sheet is an interconnected QD system, it possesses the optical properties of GQDs. Metal atoms that interconnect the GQDs in the bottom-up hydrothermal growth process, induce the semiconducting behaviour in the GQD solid sheets...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28879486/f%C3%A3-rster-resonance-energy-transfer-study-of-cytochrome-c-lipid-interactions
#19
Galyna P Gorbenko, Valeriya Trusova, Julian G Molotkovsky
Specific interactions between a mitochondrial hemoprotein cytochrome c (cyt c) and cardiolipin, a lipid component of mitochondrial membrane, are crucial to electron shuttling and apoptotic activities of this protein. In the present study the Förster resonance energy transfer (FRET) between anthrylvinyl-labeled phosphatidylcholine as a donor and heme moiety of cyt c as an acceptor was employed to give a quantitative characterization of the protein binding to the model membranes from the mixtures of phosphatidylcholine (PC) with phosphatidylglycerol (PG), phosphatidylserine (PS) or cardiolipin (CL) in different molar ratios...
September 6, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28877485/conformational-heterogeneity-and-fret-data-interpretation-for-dimensions-of-unfolded-proteins
#20
Jianhui Song, Gregory-Neal Gomes, Tongfei Shi, Claudiu C Gradinaru, Hue Sun Chan
A mathematico-physically valid formulation is required to infer properties of disordered protein conformations from single-molecule Förster resonance energy transfer (smFRET). Conformational dimensions inferred by conventional approaches that presume a homogeneous conformational ensemble can be unphysical. When all possible-heterogeneous as well as homogeneous-conformational distributions are taken into account without prejudgment, a single value of average transfer efficiency 〈E〉 between dyes at two chain ends is generally consistent with highly diverse, multiple values of the average radius of gyration 〈Rg〉...
September 5, 2017: Biophysical Journal
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