keyword
MENU ▼
Read by QxMD icon Read
search

dimerization

keyword
https://www.readbyqxmd.com/read/29160708/photophysics-of-zinc-porphyrin-aggregates-in-dilute-water-ethanol-solutions
#1
Amy L Stevens, Neeraj Kumar Joshi, Matthew F Paige, Ronald Paul Steer
Dimeric and multimeric aggregates of a model metalloporphyrin, zinc tetraphenylporphyrin (ZnTPP), have been produced in a controlled manner by incrementally increasing the water content of dilute aqueous ethanol solutions. Steady state absorption, fluorescence emission and fluorescence excitation spectra have been measured to identify the aggregates present as a function of solvent composition. The dynamics of the excited states of the aggregates produced initially by excitation in the Soret region have been measured by ultrafast fluorescence upconversion techniques...
November 21, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29160688/a-novel-real-time-proximity-assay-for-characterizing-multiple-receptor-interactions-on-living-cells
#2
Sina Bondza, Hanna Björkelund, Marika Nestor, Karl Andersson, Jos Buijs
Cellular receptor activity is often controlled through complex mechanisms involving interactions with multiple molecules, which can be soluble ligands and/or other cell surface molecules. In this study, we combine a fluorescence based technology for real-time interaction analysis with fluorescence quenching to create a novel time resolved proximity assay to study protein-receptor interactions on living cells. This assay extracts the binding kinetics and affinity for two proteins if they bind in proximity on the cell surface...
November 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29160572/rapid-on-site-evaluation-of-routine-biochemical-parameters-to-predict-right-ventricular-dysfunction-in-and-the-prognosis-of-patients-with-acute-pulmonary-embolism-upon-admission-to-the-emergency-room
#3
Dong Jia, Fan Liu, Qin Zhang, Guang-Qiao Zeng, Xue-Lian Li, Gang Hou
INTRODUCTION: Patients with acute pulmonary embolism(APE)who present with right ventricular dysfunction (RVD) have a worse prognosis. This study aimed to evaluate the value of routine biochemical parameters in predicting RVD and 30-day mortality in patients with APE. METHODS: We retrospectively collected the clinical data for 154 enrolled patients, including the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), D-dimer, cardiac troponin I (cTnI), and N-terminal pro-brain natriuretic peptide (NT-proBNP)...
November 21, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29160296/structure-of-the-fission-yeast-s-pombe-telomeric-tpz1-poz1-rap1-complex
#4
Jing Xue, Hongwen Chen, Jian Wu, Miho Takeuchi, Haruna Inoue, Yanmei Liu, Hong Sun, Yong Chen, Junko Kanoh, Ming Lei
Telomeric shelterin complex caps chromosome ends and plays a crucial role in telomere maintenance and protection. In the fission yeast Schizosaccharomyces pombe, shelterin is composed of telomeric single- and double-stranded DNA-binding protein subcomplexes Pot1-Tpz1 and Taz1-Rap1, which are bridged by their interacting protein Poz1. However, the structure of Poz1 and how Poz1 functions as an interaction hub in the shelterin complex remain unclear. Here we report the crystal structure of Poz1 in complex with Poz1-binding motifs of Tpz1 and Rap1...
November 21, 2017: Cell Research
https://www.readbyqxmd.com/read/29160227/enhanced-green-upconversion-luminescence-in-zno-er3-yb3-on-mo6-co-doping-for-temperature-sensor-application
#5
R Anjana, Subha P P, Kurias K Markose, Madambi K Jayaraj
Erbium (Er) doped zinc oxide (ZnO) powder was prepared using co-precipitation method. Effect of ytterbium (Yb) and molybdenum (Mo) co-doping on upconversion emission was studied. The emission intensity has been enhanced on Yb co-doping due to energy transfer from Yb to Er. Co-doping Mo in ZnO:Er, Yb has brought a significant enhancement in green upconversion emission, when excited with 980 nm laser. The enhancement in green emission on Mo doping is because of the formation of Yb3+-MoO42- dimer. Since ZnO is a material having high thermal stability the enhanced green emission in Mo, Er, Yb doped ZnO can be used for temperature sensor applications...
November 21, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/29160072/solvent-controlled-branching-of-localized-versus-delocalized-singlet-exciton-states-and-equilibration-with-charge-transfer-in-a-structurally-well-defined-tetracene-dimer
#6
Jasper D Cook, Thomas J Carey, Dylan H Arias, Justin C Johnson, Niels H Damrauer
A detailed photophysical picture is elaborated for a structurally well-defined and symmetrical bis-tetracene dimer in solution. The molecule was designed for interrogation of the initial photophysical steps (S1 → (1)TT) in intramolecular singlet fission (SF). (Triisopropylsilyl)acetylene substituents on the dimer TIPS-BT1 as well as a monomer model TIPS-Tc enable a comparison of photophysical properties, including transient absorption dynamics, as solvent polarity is varied. In nonpolar toluene solutions, TIPS-BT1 decays via radiative and nonradiative pathways to the ground state with no evidence for dynamics related to the initial stages of SF...
November 21, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29159349/near-field-chemical-mapping-of-gold-nanostructures-using-a-functionalized-scanning-probe
#7
C Dab, C Awada, A Merlen, A Ruediger
We report on photochemical and photophysical properties produced by Surface Plasmon Resonance (SPR) on metallic nanograins by means of high resolution Functionalized Tip-Enhanced Raman Spectroscopy (F-TERS). This technique relies on a sharp gold tip functionalized with Raman-active molecules to be scanned relatively to plasmonic hot-spots on a surface. We describe the local variation of plasmon-induced Raman enhancement on the surface of nanostructures that also affects the photochemistry while the quantitative interpretation of peak intensities requires the consideration of surface topography near the tip apex...
November 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29158434/high-levels-of-cyclic-di-gmp-in-klebsiella-pneumoniae-attenuate-virulence-in-the-lung
#8
David A Rosen, Joy Twentyman, David A Hunstad
The bacterial second messenger, bis-(3' -5')-cyclic dimeric guanosine monophosphate (c-di-GMP) has been shown to influence expression of virulence factors in certain pathogenic bacteria, but little is known about its activity in the increasingly antibiotic-resistant pathogen Klebsiella pneumoniae Here, expression in K. pneumoniae of a heterologous diguanylate cyclase increased bacterial c-di-GMP concentration and attenuated pathogenesis in murine pneumonia. This attenuation remained evident in mice lacking the c-di-GMP sensor STING, indicating that high c-di-GMP exerted its influence not on host responses, but on bacterial physiology...
November 20, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/29158389/burkholderia-cenocepacia-integrates-cis-2-dodecenoic-acid-and-cyclic-dimeric-guanosine-monophosphate-signals-to-control-virulence
#9
Chunxi Yang, Chaoyu Cui, Qiumian Ye, Jinhong Kan, Shuna Fu, Shihao Song, Yutong Huang, Fei He, Lian-Hui Zhang, Yantao Jia, Yong-Gui Gao, Caroline S Harwood, Yinyue Deng
Quorum sensing (QS) signals are used by bacteria to regulate biological functions in response to cell population densities. Cyclic diguanosine monophosphate (c-di-GMP) regulates cell functions in response to diverse environmental chemical and physical signals that bacteria perceive. In Burkholderia cenocepacia, the QS signal receptor RpfR degrades intracellular c-di-GMP when it senses the QS signal cis-2-dodecenoic acid, also called Burkholderia diffusible signal factor (BDSF), as a proxy for high cell density...
November 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29158379/energy-decomposition-analysis-of-single-bonds-within-kohn-sham-density-functional-theory
#10
Daniel S Levine, Martin Head-Gordon
An energy decomposition analysis (EDA) for single chemical bonds is presented within the framework of Kohn-Sham density functional theory based on spin projection equations that are exact within wave function theory. Chemical bond energies can then be understood in terms of stabilization caused by spin-coupling augmented by dispersion, polarization, and charge transfer in competition with destabilizing Pauli repulsions. The EDA reveals distinguishing features of chemical bonds ranging across nonpolar, polar, ionic, and charge-shift bonds...
November 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29157782/comparative-assessment-of-the-effect-of-hyper-glycosylation-on-the-pattern-and-kinetics-of-degradation-of-darbepoetin-alfa-using-a-stability-indicating-orthogonal-testing-protocol
#11
Eman M Moenes, Medhat A Al-Ghobashy, Abeer A Mohamed, Maissa Y Salem
Darbepoetin alfa (DA); hyper-glycosylated Erythropoietin alfa (EPO) is an essential treatment of anemia in patients with chronic kidney failure and cancer. In this study, DA and EPO were subjected to physicochemical stress factors that might be encountered during production, transport and storage (pH, temperature, agitation, repeated freeze-thaw and oxidation). An orthogonal stability-indicating assay protocol comprised of SE-HPLC, RP-HPLC, ELISA and SDS-PAGE was developed and validated to investigate the effect of further glycosylation of DA on the pattern and kinetics of degradation...
November 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29157189/molecular-dynamics-of-a-far-positioned-sod1-mutant-v14m-reveals-pathogenic-misfolding-behavior
#12
Dharma Rao Tompa, K Saraboji
Human superoxide dismutase (Cu/Zn SOD1) is a homodimeric enzyme. Mutations in Cu/Zn SOD1 causes a familial form of amyotrophic lateral sclerosis (fALS), and aggregation of mutant SOD1 has been proposed to play a role in neurodegeneration. Though a majority of the mutations are point substitutions, there are a few changes that result in amino acid deletions or truncations of the polypeptide. These pathogenic mutations are scattered throughout the three-dimensional structure of the dimeric enzyme, which creates a puzzling pattern to investigate the molecular determinants of fALS...
November 20, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29156316/neutral-dipole-dipole-dimers-a-new-field-in-science
#13
Edward M Kosower, Galina Borz
Dimer formation with dipole neutralization produces species such as low polarity water (LPW) compatible with hydrophobic surfaces (Phys. Chem. Chem. Phys. 2015, 17, 24895-24900) Dimerization and dipole neutralization occurs for N-methylacetamide on polyethylene, a behavior drastically different from its contortions in acetonitrile on AgBr:AgCl planar crystals (AgX) (ChemPhysChem 2014, 15, 3598-3607). The weak infrared absorption of the amide dimer on polyethylene is shown experimentally. Dimerization of palmitic acid is shown along with some of the many ramifications for intracellular systems...
November 11, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29156124/definition-of-free-o-h-groups-of-water-at-the-air-water-interface
#14
Fujie Tang, Tatsuhiko Ohto, Taisuke Hasegawa, Wen Jun Xie, Limei Xu, Mischa Bonn, Yuki Nagata
Free O-H groups of water are often found at the water-hydrophobic medium interface, e.g. for water contact with hydrophobic protein residues, or at the water-air interface. In surface-specific vibrational spectroscopic studies using sum-frequency generation (SFG) spectroscopy, free O-H groups are experimentally well characterized in the O-H stretch region by a sharp 3700 cm(-1) peak. Although these free O-H groups are often defined as the O-H groups which are not hydrogen-bonded to other water molecules, a direct correlation between such non-hydrogen-bonded O-H group and the 3700 cm(-1) SFG response has been lacking...
November 20, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/29156095/multiple-role-of-3-mercaptopyruvate-sulfurtransferase-antioxidative-function-h2-s-and-polysulfide-production-and-possible-sox-production
#15
REVIEW
Noriyuki Nagahara
Rat 3-mercaptopyruvate sulfurtransferase (MST, [EC 2.8.1.2]) is a 32,808-Da simple protein. Cys(247) is a catalytic site, and Cys(154) and Cys(263) are on the enzyme surface. MST is found in all tissues, particularly in the kidneys, although activity localization differs in each tissue. In this review, four functions of MST are reviewed. 1) Antioxidative function: Cys(247) is redox-sensitive and serves as a redox-sensing switch. It is oxidized to cysteine sulfenate, which has a low redox potential, upon which the enzyme is inactivated...
November 20, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29155820/mass-spectrometry-based-quantification-of-the-cellular-response-to-ultraviolet-radiation-in-hela-cells
#16
Hong Xu, Xuanyi Chen, Nanjiao Ying, Meixia Wang, Xiaoli Xu, Rongyi Shi, Yuejin Hua
Ultraviolet (UV) irradiation is a common form of DNA damage that can cause pyrimidine dimers between DNA, which can cause gene mutations, even double-strand breaks and threaten genome stability. If DNA repair systems default their roles at this stage, the organism can be damaged and result in disease, especially cancer. To better understand the cellular response to this form of damage, we applied highly sensitive mass spectrometry to perform comparative proteomics of phosphorylation in HeLa cells. A total of 4367 phosphorylation sites in 2100 proteins were identified, many of which had not been reported previously...
2017: PloS One
https://www.readbyqxmd.com/read/29155761/advanced-confocal-microscopy-techniques-to-study-protein-protein-interactions-and-kinetics-at-dna-lesions
#17
Soňa Legartová, Jana Suchánková, Jana Krejčí, Alena Kovaříková, Eva Bártová
Local microirradiation with lasers represents a useful tool for studies of DNA-repair-related processes in live cells. Here, we describe a methodological approach to analyzing protein kinetics at DNA lesions over time or protein-protein interactions on locally microirradiated chromatin. We also show how to recognize individual phases of the cell cycle using the Fucci cellular system to study cell-cycle-dependent protein kinetics at DNA lesions. A methodological description of the use of two UV lasers (355 nm and 405 nm) to induce different types of DNA damage is also presented...
November 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155581/phosphorescence-control-mediated-by-molecular-rotation-and-aurophilic-interactions-in-amphidynamic-crystals-of-1-4-bis-tri-p-fluorophenyl-phosphane-gold-i-ethynyl-benzene
#18
Mingoo Jin, Tim S Chung, Tomohiro Seki, Hajime Ito, Miguel A Garcia-Garibay
Here we present a structural design aimed at the control of phosphorescence emission as the result of changes in molecular rotation in a crystalline material. The proposed strategy includes the use of aurophilic interactions, both as a crystal engineering tool and as sensitive emission probe, and the use of a dumbbell-shaped architecture intended to create a low packing density region that permits the rotation of a central phenylene. Molecular rotor 1 with of a central 1,4-diethynylphenylene rotator linked to two gold(I) triphenylphosphane complexes was prepared and its structure confirmed by single crystal X-ray diffraction (XRD), which revealed chains mediated by dimeric aurophilic interactions...
November 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29155560/asymmetric-assembling-of-iron-oxide-nanocubes-for-improving-magnetic-hyperthermia-performance
#19
Dina Niculaes, Aidin Lak, George C Anyfantis, Sergio Marras, Oliver Laslett, Sahitya K Avugadda, Marco Cassani, David Serantes, Ondrej Hovorka, Roy Chantrell, Teresa Pellegrino
agnetic hyperthermia (MH) based on magnetic nanoparticles (MNPs) is a promising adjuvant therapy for cancer treatment. Particle clustering leading to complex magnetic interactions affects the heat generated by MNPs during MH. The heat efficiencies, theoretically predicted, are still poorly understood because of lack of the control of the fabrication of such clusters with defined geometries and thus their functionality. This study aims to correlate the heating efficiency under MH of individually coated iron oxide nanocubes (IONCs) vs...
November 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/29155493/the-crystal-structure-and-catalytic-mechanism-of-hydroxynitrile-lyase-from-passion-fruit-passiflora-edulis
#20
Fumihiro Motojima, Aem Nuylert, Yasuhisa Asano
Hydroxynitrile lyases (HNLs) are enzymes used in the synthesis of chiral cyanohydrins. The HNL from Passiflora edulis (PeHNL) is R-selective and is the smallest HNL known to date. The crystal structures of PeHNL and its C-terminal peptide depleted derivative were determined by molecular replacement method using the template structure of a heat stable protein, SP1, from Populus tremula at 2.8 Å and 1.8 Å resolution, respectively. PeHNL belongs to dimeric α+β barrel superfamily consisting of a central β-barrel in the middle of a dimer...
November 20, 2017: FEBS Journal
keyword
keyword
46994
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"