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https://www.readbyqxmd.com/read/29037939/relationship-of-hospital-teaching-status-with-in-hospital-outcomes-for-st-segment-elevation-myocardial-infarction
#1
Tanush Gupta, Kavisha Patel, Dhaval Kolte, Sahil Khera, Pedro A Villablanca, Wilbert S Aronow, William H Frishman, Howard A Cooper, Anna E Bortnick, Gregg C Fonarow, Julio A Panza, Giora Weisz, Mark A Menegus, Mario J Garcia, Deepak L Bhatt
BACKGROUND: Prior analyses have largely shown a survival advantage with admission to a teaching hospital for acute myocardial infarction. However, most prior studies report data on patients hospitalized over a decade ago. It is important to re-examine the association of hospital teaching status with outcomes of acute myocardial infarction in the current era. METHODS: We queried the 2010 to 2014 National Inpatient Sample databases to identify all patients aged ≥18 years hospitalized with the principal diagnosis of ST-segment elevation myocardial infarction (STEMI)...
October 13, 2017: American Journal of Medicine
https://www.readbyqxmd.com/read/29026596/flow-cytometry-of-duodenal-intraepithelial-lymphocytes-improves-diagnosis-of-celiac-disease-in-difficult-cases
#2
Julio Valle, José Mario T Morgado, Juan Ruiz-Martín, Antonio Guardiola, Miriam Lopes-Nogueras, Almudena García-Vela, Beatriz Martín-Sacristán, Laura Sánchez-Muñoz
BACKGROUND: Diagnosis of celiac disease is difficult when the combined results of serology and histology are inconclusive. Studies using flow cytometry of intraepithelial lymphocytes (IELs) have found that celiac patients have increased numbers of γδ IELs, along with a decrease in CD3-CD103 + IELs. OBJECTIVE: The objective of this article is to assess the role of flow cytometric analysis of IELs in the diagnosis of celiac disease in difficult cases. METHODS: A total of 312 patients with suspicion of celiac disease were included in the study...
October 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/29025667/adhesion-forces-of-biofilms-developed-in-vitro-from-clinical-strains-of-skin-wounds
#3
Elizabeth Alvarado-Gomez, Mario Perez-Diaz, Donato Valdez-Perez, Jaime Ruiz-Garcia, Martin Magaña-Aquino, Gabriel Martinez-Castañon, Fidel Martinez-Gutierrez
A biofilm is a very complex consortium formed by a mix of different microorganisms, which have become an important health problem, because its formation is a resistance mechanism used by bacteria against antibiotics or the immune system. In this work, we show differences between some physicochemical properties of biofilms in mono- and multi-species, formed by bacteria from clinical samples of infected chronic wounds. Of the most prevalent bacteria in wounds, two mono- and one multi-species biofilms were developed in vitro by Drip Flow Reactor: one biofilm was developed by S...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29023707/guidelines-for-the-use-of-flow-cytometry-and-cell-sorting-in-immunological-studies
#4
Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Immanuel Andrä, Francesco Annunziato, Petra Bacher, Vincenzo Barnaba, Luca Battistini, Wolfgang M Bauer, Sabine Baumgart, Burkhard Becher, Wolfgang Beisker, Claudia Berek, Alfonso Blanco, Giovanna Borsellino, Philip E Boulais, Ryan R Brinkman, Martin Büscher, Dirk H Busch, Timothy P Bushnell, Xuetao Cao, Andrea Cavani, Pratip K Chattopadhyay, Qingyu Cheng, Sue Chow, Mario Clerici, Anne Cooke, Antonio Cosma, Lorenzo Cosmi, Ana Cumano, Van Duc Dang, Derek Davies, Sara De Biasi, Genny Del Zotto, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, Andreas Diefenbach, James Di Santo, Francesco Dieli, Andreas Dolf, Vera S Donnenberg, Thomas Dörner, Götz R A Ehrhardt, Elmar Endl, Pablo Engel, Britta Engelhardt, Charlotte Esser, Bart Everts, Christine S Falk, Todd A Fehniger, Andrew Filby, Simon Fillatreau, Marie Follo, Irmgard Förster, John Foster, Gemma A Foulds, Paul S Frenette, David Galbraith, Natalio Garbi, Maria Dolores García-Godoy, Kamran Ghoreschi, Lara Gibellini, Christoph Goettlinger, Carl S Goodyear, Andrea Gori, Jane Grogan, Mor Gross, Andreas Grützkau, Daryl Grummitt, Jonas Hahn, Quirin Hammer, Anja E Hauser, David L Haviland, David Hedley, Guadalupe Herrera, Martin Herrmann, Falk Hiepe, Tristan Holland, Pleun Hombrink, Jessica P Houston, Bimba F Hoyer, Bo Huang, Christopher A Hunter, Anna Iannone, Hans-Martin Jäck, Beatriz Jávega, Stipan Jonjic, Kerstin Juelke, Steffen Jung, Toralf Kaiser, Tomas Kalina, Baerbel Keller, Srijit Khan, Deborah Kienhöfer, Thomas Kroneis, Désirée Kunkel, Christian Kurts, Pia Kvistborg, Joanne Lannigan, Olivier Lantz, Anis Larbi, Salome LeibundGut-Landmann, Michael D Leipold, Megan K Levings, Virginia Litwin, Yanling Liu, Michael Lohoff, Giovanna Lombardi, Lilly Lopez, Amy Lovett-Racke, Erik Lubberts, Burkhard Ludewig, Enrico Lugli, Holden T Maecker, Glòria Martrus, Giuseppe Matarese, Christian Maueröder, Mairi McGrath, Iain McInnes, Henrik E Mei, Fritz Melchers, Susanne Melzer, Dirk Mielenz, Kingston Mills, Jenny Mjösberg, Jonni Moore, Barry Moran, Alessandro Moretta, Lorenzo Moretta, Tim R Mosmann, Susann Müller, Werner Müller, Christian Münz, Gabriele Multhoff, Luis Enrique Munoz, Kenneth M Murphy, Toshinori Nakayama, Milena Nasi, Christine Neudörfl, John Nolan, Sussan Nourshargh, José-Enrique O'Connor, Wenjun Ouyang, Annette Oxenius, Raghav Palankar, Isabel Panse, Pärt Peterson, Christian Peth, Jordi Petriz, Daisy Philips, Winfried Pickl, Silvia Piconese, Marcello Pinti, A Graham Pockley, Malgorzata Justyna Podolska, Carlo Pucillo, Sally A Quataert, Timothy R D J Radstake, Bartek Rajwa, Jonathan A Rebhahn, Diether Recktenwald, Ester B M Remmerswaal, Katy Rezvani, Laura G Rico, J Paul Robinson, Chiara Romagnani, Anna Rubartelli, Jürgen Ruland, Shimon Sakaguchi, Francisco Sala-de-Oyanguren, Yvonne Samstag, Sharon Sanderson, Birgit Sawitzki, Alexander Scheffold, Matthias Schiemann, Frank Schildberg, Esther Schimisky, Stephan A Schmid, Steffen Schmitt, Kilian Schober, Thomas Schüler, Axel Ronald Schulz, Ton Schumacher, Cristiano Scotta, T Vincent Shankey, Anat Shemer, Anna-Katharina Simon, Josef Spidlen, Alan M Stall, Regina Stark, Christina Stehle, Merle Stein, Tobit Steinmetz, Hannes Stockinger, Yousuke Takahama, Attila Tarnok, ZhiGang Tian, Gergely Toldi, Julia Tornack, Elisabetta Traggiai, Joe Trotter, Henning Ulrich, Marlous van der Braber, René A W van Lier, Marcello Veldhoen, Salvador Vento-Asturias, Paulo Vieira, David Voehringer, Hans-Dieter Volk, Konrad von Volkmann, Ari Waisman, Rachael Walker, Michael D Ward, Klaus Warnatz, Sarah Warth, James V Watson, Carsten Watzl, Leonie Wegener, Annika Wiedemann, Jürgen Wienands, Gerald Willimsky, James Wing, Peter Wurst, Liping Yu, Alice Yue, Qianjun Zhang, Yi Zhao, Susanne Ziegler, Jakob Zimmermann
No abstract text is available yet for this article.
October 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29023104/quantitative-evaluation-of-cisplatin-uptake-in-sensitive-and-resistant-individual-cells-by-single-cell-icp-ms-sc-icp-ms
#5
Mario Corte Rodríguez, Roberto Álvarez-Fernández García, Elisa Blanco Gonzalez, Jorg Bettmer, Maria Montes-Bayon
One of the main limitations to the Pt-therapy in cancer is the development of associated drug resistance that can be associated to a significant reduction of the intracellular platinum concentration. Thus, intracellular Pt concentration could be considered as a biomarker of cisplatin resistance. In this work, an alternative method to address intracellular Pt concentration in individual cells is explored to permit the evaluation of different cell models and/or alternative therapies in a relatively fast way. For this aim, total Pt analysis in single cells has been implemented using a total consumption nebulizer coupled to inductively coupled plasma mass spectrometric detection (ICP-MS)...
October 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29022223/acute-kidney-injury-in-pregnancy-and-the-role-of-underlying-ckd-a-point-of-view-from-m%C3%A3-xico
#6
REVIEW
Margarita Ibarra-Hernández, Oralia Alejandra Orozco-Guillén, María Luz de la Alcantar-Vallín, Ruben Garrido-Roldan, María Patricia Jiménez-Alvarado, Kenia Benitez Castro, Francisco Villa-Villagrana, Mario Borbolla, Juan Manuel Gallardo-Gaona, Guillermo García-García, Norberto Reyes-Paredes, Giorgina Barbara Piccoli
The incidence of acute kidney injury in pregnancy (P-AKI) in developed countries is significantly lower than in developing ones, where it is estimated to range between 4 and 26%. Mortality in cases of P-AKI requiring dialysis is high, varying from 20 to 80%. In developing countries, clinical decisions are often based on the availability of services and not on needs. Prenatal surveillance in Mexico does not include serum creatinine, limiting the potential for early diagnosis of AKI and CKD and their differential diagnosis...
October 11, 2017: Journal of Nephrology
https://www.readbyqxmd.com/read/29019580/effects-of-local-pressure-on-cutaneous-blood-flow-in-pigs
#7
Michel Luciano Holger Toledano Vaena, João Paulo Sinnecker, Bruno Benedetti Pinto, Mario Fritsch Toros Neves, Fernando Serra-Guimarães, Ruy Garcia Marques
OBJECTIVE: to evaluate the effects of increasing pressures on the cutaneous blood flow in the skin of pigs. METHODS: we conducted an experimental study in pigs submitted to subcutaneous magnetic implants (n=30). After healing, were applied external magnets with varying magnetic forces to the skin, generating compression. We evaluated the cutaneous circulation of the skin under compression by the Laser Speckle Contrast Imaging (LSCI) technique. We measured the depth of the implants by ultrasonography, and applied computational simulations to the calculation of the different pressure values, considering the different distances between implants and external magnets...
September 2017: Revista do Colégio Brasileiro de Cirurgiões
https://www.readbyqxmd.com/read/28991277/centro-de-trasplante-hep%C3%A3-tico-en-m%C3%A3-xico-con-bajo-volumen-y-excelentes-resultados
#8
Mario Vilatobá, Miguel Ángel Mercado, Alan Gabriel Contreras-Saldivar, Rafael Paulino Leal-Villalpando, Jorge Zamudio-Bautista, Ignacio García-Juárez, Armando Gamboa-Domínguez
other: El trasplante hepático ortotópico (THO) es el tratamiento de elección para la insuficiencia hepática terminal. Numerosos estudios muestran una relación inversa entre el número de procedimientos y la mortalidad operatoria. El objetivo de este estudio es mostrar los resultados de nuestro centro y determinar si puede tener resultados equiparables a los obtenidos en centros de alto volumen. Es un estudio retrospectivo en el que se analizó la información de pacientes con THO en nuestra institución, de 1985 al 31 de diciembre de 2012...
2017: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
https://www.readbyqxmd.com/read/28988671/c3-deposits-worsens-the-prognosis-in-type-iii-extracapillary-glomerulonephritis
#9
Marina Sánchez-Agesta Martínez, Cristina Rabasco Ruiz, Rafael Sánchez Sánchez, Rosa Ortega Salas, María López Andreu, Pedro Aljama García, Mario Espinosa Hernández
INTRODUCTION: Type iii extracapillary glomerulonephritis (PEGN) is a common cause of rapidly progressive glomerulonephritis and it is usually associated with circulating anti-neutrophil cytoplasmic antibodies (ANCAs). Recent evidence points to complement activation as an important factor in the pathogenesis of PEGN. The aim of the present study was to assess the value of C3 deposits in the prognosis of PEGN. METHODS: All patients diagnosed of PEGN from 1995 to 2015 (n=72) were included in this study...
October 5, 2017: Nefrología: Publicación Oficial de la Sociedad Española Nefrologia
https://www.readbyqxmd.com/read/28978170/imatinib-in-systemic-mastocytosis-a-phase-iv-clinical-trial-in-patients-lacking-exon-17-kit-mutations-and-review-of-the-literature
#10
Iván Alvarez-Twose, Almudena Matito, José Mário Morgado, Laura Sánchez-Muñoz, María Jara-Acevedo, Andrés García-Montero, Andrea Mayado, Carolina Caldas, Cristina Teodósio, Javier Ignacio Muñoz-González, Manuela Mollejo, Luis Escribano, Alberto Orfao
Resistance to imatinib has been recurrently reported in systemic mastocytosis (SM) carrying exon 17 KIT mutations. We evaluated the efficacy and safety of imatinib therapy in 10 adult SM patients lacking exon 17 KIT mutations, 9 of which fulfilled criteria for well-differentiated SM (WDSM). The World Health Organization 2008 disease categories among WDSM patients were mast cell (MC) leukemia (n = 3), indolent SM (n = 3) and cutaneous mastocytosis (n = 3); the remainder case had SM associated with a clonal haematological non-MC disease...
September 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28975665/crystal-structures-of-staphylococcus-aureus-ketol-acid-reductoisomerase-in-complex-with-two-transition-state-analogs-that-have-biocidal-activity
#11
Khushboo Patel, David Teran, Shan Zheng, Ajit Kandale, Mario Garcia, You Lv, Mark A Schembri, Ross P McGeary, Gerhard Schenk, Luke William Guddat
Ketol-acid reductoisomerase (KARI) is an NAD(P)H and Mg2+-dependent enzyme of the branched-chain amino acid (BCAA) biosynthesis pathway. Here we describe the first crystal structures of Staphylococcus aureus (Sa) KARI, these in complex with two transition state analogs, cyclopropane-1,1-dicarboxylate (CPD) and N-isopropyloxalyl hydroxamate (IpOHA). These compounds bind competitively and in multi-dentate manner to KARI with Ki values of 2.73 μM and 7.9 nM, respectively; however, IpOHA binds slowly to the enzyme...
October 4, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28974003/small-molecules-derived-from-thieno-3-4-c-pyrrole-4-6-dione-tpd-and-their-use-in-solution-processed-organic-solar-cells
#12
Cesar Garcias-Morales, Daniel Romero-Borja, José-Luis Maldonado, Arián E Roa, Mario Rodríguez, J Pablo García-Merinos, Armando Ariza-Castolo
In this work, microwave synthesis, chemical, optical and electrochemical characterization of three small organic molecules, TPA-TPD, TPA-PT-TPD and TPA-TT-TPD with donor-acceptor structure and their use in organic photovoltaic cells are reported. For the synthesis, 5-(2-ethylhexyl)-4H-thieno[3,4-c]pyrrole-4,6(5H)-dione was used as electron withdrawing fragment while the triphenylamine was used as electron donating fragment. Molecular electronic geometry and electronic distribution density were established by density functional theory (DFT) calculations and confirmed by optical and chemical characterization...
September 30, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28972965/hyaluronic-acid-family-in-bladder-cancer-potential-prognostic-biomarkers-and-therapeutic-targets
#13
Daley S Morera, Martin S Hennig, Asif Talukder, Soum D Lokeshwar, Jiaojiao Wang, Michael Garcia-Roig, Nicolas Ortiz, Travis J Yates, Luis E Lopez, Georgios Kallifatidis, Mario W Kramer, Andre R Jordan, Axel S Merseburger, Murugesan Manoharan, Mark S Soloway, Martha K Terris, Vinata B Lokeshwar
BACKGROUND: Molecular markers of clinical outcome may aid in designing targeted treatments for bladder cancer. However, only a few bladder cancer biomarkers have been examined as therapeutic targets. METHODS: Data from The Cancer Genome Atlas (TCGA) and bladder specimens were evaluated to determine the biomarker potential of the hyaluronic acid (HA) family of molecules - HA synthases, HA receptors and hyaluronidase. The therapeutic efficacy of 4-methylumbelliferone (4MU), a HA synthesis inhibitor, was evaluated in vitro and in xenograft models...
October 3, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/28971731/absence-of-measles-virus-detection-from-stapes-of-patients-with-otosclerosis
#14
María de Lourdes Flores-García, Claudia Adriana Colín-Castro, Mario Sabas Hernández-Palestina, Roberto Sánchez-Larios, Rafael Franco-Cendejas
Objective To determine molecularly the presence of measles virus genetic material in the stapes of patients with otosclerosis. Study Design A cross-sectional study. Setting A tertiary referral hospital. Subjects and Methods Genetic material was extracted from the stapes of patients with otosclerosis (n = 93) during the period from March 2011 to April 2012. The presence of viral measles sequences was evaluated by the real-time reverse transcriptase polymerase chain reaction (RT-PCR). The expression of the CD46 gene was determined...
September 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28964831/discovery-of-novel-non-competitive-inhibitors-of-mammalian-neutral-m1-aminopeptidase-apn
#15
Isel Pascual, Pedro A Valiente, Gabriela García, Mario E Valdés-Tresanco, Yarini Arrebola, Lisset Díaz, Lotfi Bounaadja, Rosa María Uribe, Mae Chappé Pacheco, Isabelle Florent, Jean-Louis Charli
Neutral metallo-aminopeptidase (APN) catalyzes the cleavage of neutral and basic amino acids from the N-terminus of protein or peptide substrates. APN expression is dysregulated in inflammatory diseases as well as in several types of cancer. Therefore, inhibitors of APN may be effective against cancer and inflammation. By virtual screening and enzymatic assays, we identified three non-competitive inhibitors (α > 1) of the porcine and human APN with Ki values in the μM range. These non-peptidic compounds lack the classical zinc-binding groups (ZBG) present in most of the APN inhibitors...
September 27, 2017: Biochimie
https://www.readbyqxmd.com/read/28963820/genomic-profiling-of-intestinal-type-sinonasal-adenocarcinoma-reveals-subgroups-of-patients-with-distinct-clinical-outcomes
#16
Alejandro López-Hernández, Jhudit Pérez-Escuredo, Blanca Vivanco, Cristina García-Inclán, Sira Potes-Ares, Virginia N Cabal, Cristina Riobello, María Costales, Fernando López, José Luis Llorente, Mario A Hermsen
BACKGROUND: Patients with intestinal-type sinonasal adenocarcinoma (ITAC) have an unfavorable prognosis and new therapeutic approaches are needed to improve clinical management. METHODS: Genetic analysis of 96 ITACs was performed by microarray comparative genomic hybridization and immunohistochemistry and correlated to previously obtained mutation, methylation, and protein expression data, and with pathological characteristics and clinical outcome. RESULTS: Seven copy number alterations (CNAs) were significantly associated with unfavorable clinical outcome: gains at 1q22-23, 3q28-29, 6p22, and 13q31-33, and losses at 4p15-16, 4q32-35, and 10q24...
September 30, 2017: Head & Neck
https://www.readbyqxmd.com/read/28958733/identification-of-bacillus-thuringiensis-cry1abmod-binding-proteins-from-spodoptera-frugiperda
#17
Diana L Martínez de Castro, Blanca I García-Gómez, Isabel Gómez, Alejandra Bravo, Mario Soberón
Bacillus thuringiensis Cry toxins are currently used for pest control in transgenic crops but evolution of resistance by the insect pests threatens the use of this technology. The Cry1AbMod toxin was engineered to lack the alpha helix-1 of the parental Cry1Ab toxin and was shown to counter resistance to Cry1Ab and Cry1Ac toxins in different insect species including the fall armyworm Spodoptera frugiperda. In addition, Cry1AbMod showed enhanced toxicity to Cry1Ab-susceptible S. frugiperda populations. To gain insights into the mechanisms of this Cry1AbMod-enhanced toxicity, we isolated the Cry1AbMod toxin binding proteins from S...
September 25, 2017: Peptides
https://www.readbyqxmd.com/read/28954077/cacipacore-virus-as-an-emergent-mosquito-borne-flavivirus
#18
Mario Luis Garcia de Figueiredo, Alberto Anastacio Amarilla, Glauciane Garcia de Figueiredo, Helda Liz Alfonso, Veronica Lippi, Felipe Gonçalves Motta Maia, Felipe Alves Morais, Cristóvão Alves da Costa, Dyana Alves Henriques, Edison Luis Durigon, Luiz Tadeu Moraes Figueiredo, Victor Hugo Aquino
INTRODUCTION: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons...
July 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28948065/genetic-alterations-of-idh1-and-vegf-in-brain-tumors
#19
Silvia Veganzones, Virginia de la Orden, Lucía Requejo, Beatriz Mediero, María Luisa González, Náyade Del Prado, Carmen Rodríguez García, Raquel Gutiérrez-González, Alvaro Pérez-Zamarrón, Armando Martínez, Marisa L Maestro, Horacio Mario Zimman, Anna González-Neira, Jesús Vaquero, Gregorio Rodríguez-Boto
BACKGROUND: This study evaluates the presence of R132H mutation in isocitrate dehydrogenase (IDH1) gene and the vascular endothelial growth factor (VEGF) +936 C/T polymorphism in brain tumors. The impact of these genetic alterations on overall survival (OS) and progression free survival (PFS) was evaluated. METHODS: A cohort of 80 patients surgically treated at Hospital Clínico San Carlos, Madrid, between March 2004 and November 2012, was analyzed. Tumors were distributed in 73 primary brain tumors (gliomas, meningiomas, hemangiopericytomas and hemangioblastomas) and seven secondary tumors evolved from a low grade glioma, thus providing a mixed sample...
September 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28933733/melatonin-decreases-glucose-metabolism-in-prostate-cancer-cells-a-13-c-stable-isotope-resolved-metabolomic-study
#20
David Hevia, Pedro Gonzalez-Menendez, Mario Fernandez-Fernandez, Sergio Cueto, Pablo Rodriguez-Gonzalez, Jose I Garcia-Alonso, Juan C Mayo, Rosa M Sainz
The pineal neuroindole melatonin exerts an exceptional variety of systemic functions. Some of them are exerted through its specific membrane receptors type 1 and type 2 (MT1 and MT2) while others are mediated by receptor-independent mechanisms. A potential transport of melatonin through facilitative glucose transporters (GLUT/SLC2A) was proposed in prostate cancer cells. The prostate cells have a particular metabolism that changes during tumor progression. During the first steps of carcinogenesis, oxidative phosphorylation is reactivated while the switch to the "Warburg effect" only occurs in advanced tumors and in the metastatic stage...
July 26, 2017: International Journal of Molecular Sciences
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