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https://www.readbyqxmd.com/read/28802100/influence-of-il-6-il-33-and-tnf-%C3%AE-on-human-mast-cell-activation-lessons-from-single-cell-analysis-by-flow-cytometry
#1
Nathalie Cop, Didier G Ebo, Chris H Bridts, Jessy Elst, Margo M Hagendorens, Christel Mertens, Margaretha A Faber, Luc S De Clerck, Vito Sabato
BACKGROUND: Mechanisms that govern priming and degranulation of human mast cells (MCs) remain elusive. Besides, most of our knowledge is based on experiments from which data only reflect an average of all stimulated cells. This study aims at investigating the effects of proinflammatory cytokines IL-6, IL-33, and TNF-α on IgE-dependent and IgE-independent activation of individual MCs. METHODS: MCs were derived from CD34(+) progenitors isolated from 50 mL whole blood from 4 healthy controls and 5 birch pollen allergic patients...
August 12, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28658502/immediate-moxifloxacin-hypersensitivity-is-there-more-than-currently-meets-the-eye
#2
A L Van Gasse, V Sabato, A P Uyttebroek, J Elst, M A Faber, M M Hagendorens, C Mertens, C H Bridts, L S De Clerck, D G Ebo
Immediate drug hypersensitivity reactions (IDHR) to moxifloxacin constitute a pathomechanistic conundrum and a diagnostic challenge. Our objective was to study whether simultaneous phenotyping and quantification of histamine release might add to our knowledge about the basophil activation properties of moxifloxacin and constitute a reliable diagnostic aid. Fifteen patients with an IDHR to moxifloxacin and nine moxifloxacin challenged controls were selected. All had a basophil activation test (BAT) with moxifloxacin...
June 28, 2017: Allergy
https://www.readbyqxmd.com/read/28655384/altered-basophil-function-in-patients-with-chronic-kidney-disease-on-hemodialysis%C3%A2
#3
Zenib Aljadi, Anna Nopp, Ola Winqvist, Aman Russom, Britta Hylander, Stefan Jacobson, Joachim Lundahl
AIMS: Chronic kidney disease (CKD) leads to impairment of immune cell function. Given the potential role of basophils in the pathogenesis of CKD, we aimed to study the basophil responsiveness towards microbial antigen exposure, judged as adhesion molecule expression and degranulation, in CKD patients on hemodialysis. MATERIALS AND METHODS: We selected markers linked to two crucial biological phases: the transmigration and degranulation processes, respectively. For the transmigration process, we selected the adhesion molecules CD11b, active CD11b epitope, and CD62L and for the degranulation process CD203c (piecemeal degranulation marker), CD63 (degranulation marker), and CD300a (inhibitory marker of degranulation)...
June 28, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28474352/airway-basophils-are-increased-and-activated-in-eosinophilic-asthma
#4
Yoshihiro Suzuki, Keiko Wakahara, Tomoko Nishio, Satoru Ito, Yoshinori Hasegawa
BACKGROUND: The impact of basophils on asthma pathogenesis remains largely unexplored, particularly in humans. Here, we evaluated the frequencies and activation status of basophils in the sputum of adult asthmatic patients and related our findings to other parameters of eosinophilic airway inflammation. METHODS: We enrolled 44 adult asthmatic patients who were being treated with inhaled corticosteroids (ICS). Analysis of the induced sputum, exhaled nitric oxide fraction (FeNO) measurement, and Asthma Control Test (ACT) were carried out together with standard blood and pulmonary function tests...
May 5, 2017: Allergy
https://www.readbyqxmd.com/read/28454740/analysis-of-basophil-activation-in-patients-with-aspirin-exacerbated-respiratory-disease
#5
Chihiro Mitsui, Keiichi Kajiwara, Emiko Ono, Kentaro Watai, Hiroaki Hayashi, Yosuke Kamide, Yuma Fukutomi, Kiyoshi Sekiya, Takahiro Tsuburai, Kazuhiko Yamamoto, Masami Taniguchi
Basophils are a major source of cysteinyl leukotrienes, but little is known about their association with the pathogenesis of aspirin-exacerbated respiratory disease (AERD). We assessed CD203c expression on peripheral basophils of patients with AERD.
April 25, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28428897/acute-myeloid-leukemia-with-basophilic-differentiation-transformed-from-myelodysplastic-syndrome
#6
Yasuhiro Tanaka, Atsushi Tanaka, Akiko Hashimoto, Kumiko Hayashi, Isaku Shinzato
Myelodysplastic syndrome (MDS) terminally transforms to acute myeloid leukemia (AML) or bone marrow failure syndrome, but acute myeloid leukemia with basophilic differentiation has been rarely reported. An 81-year-old man was referred to our department for further examination of intermittent fever and normocytic anemia during immunosuppressive treatment. Chromosomal analysis showed additional abnormalities involving chromosome 7. He was diagnosed as having MDS. At the time of diagnosis, basophils had not proliferated in the bone marrow...
2017: Case Reports in Hematology
https://www.readbyqxmd.com/read/28418072/myb-gata1-fusion-promotes-basophilic-leukaemia-involvement-of-interleukin-33-and-nerve-growth-factor-receptors
#7
Stéphane Ducassou, Valérie Prouzet-Mauléon, Marie-Céline Deau, Philippe Brunet de la Grange, Bruno Cardinaud, Hayssam Soueidan, Cathy Quelen, Pierre Brousset, Jean-Max Pasquet, François Moreau-Gaudry, Michel Arock, François-Xavier Mahon, Eric Lippert
Acute basophilic leukaemia (ABL) is a rare subtype of acute myeloblastic leukaemia. We previously described a recurrent t(X;6)(p11;q23) translocation generating an MYB-GATA1 fusion gene in male infants with ABL. To better understand its role, the chimeric MYB-GATA1 transcription factor was expressed in CD34-positive haematopoietic progenitors, which were transplanted into immunodeficient mice. Cells expressing MYB-GATA1 showed increased expression of markers of immaturity (CD34), of granulocytic lineage (CD33 and CD117), and of basophilic differentiation (CD203c and FcϵRI)...
April 18, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28396318/blocking-allergic-reaction-through-targeting-surface-bound-ige-with-low-affinity-anti-ige-antibodies
#8
Ke Zhang, Jeffrey Liu, Thao Truong, Elyssa Zukin, Wendy Chen, Andrew Saxon
Allergic disorders have now become a major worldwide public health issue, but the effective treatment options remain limited. We report a novel approach to block allergic reactivity by targeting the surface-bound IgE of the allergic effector cells via low-affinity anti-human IgE Abs with dissociation constants in the 10(-6) to 10(-8) M range. We demonstrated that these low-affinity anti-IgE mAbs bind to the cell surface-bound IgE without triggering anaphylactic degranulation even at high concentration, albeit they would weakly upregulate CD203c expression on basophils...
May 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28342911/basophils-high-affinity-ige-receptors-and-ccl2-in-human-anaphylaxis
#9
Peter Korosec, Paul J Turner, Mira Silar, Peter Kopac, Mitja Kosnik, Bernhard F Gibbs, Mohamed H Shamji, Adnan Custovic, Matija Rijavec
BACKGROUND: The role of basophils in anaphylaxis is unclear. OBJECTIVE: We sought to investigate whether basophils have an important role in human anaphylaxis. METHODS: In an emergency department study we recruited 31 patients with acute anaphylaxis, predominantly to Hymenoptera venom. We measured expression of basophil activation markers (CD63 and CD203c); the absolute number of circulating basophils; whole-blood FCER1A, carboxypeptidase A3 (CPA3), and L-histidine decarboxylase (HDC) gene expression; and serum markers (CCL2, CCL5, CCL11, IL-3, and thymic stromal lymphopoietin) at 3 time points (ie, during the anaphylactic episode and in convalescent samples 7 and 30 days later)...
March 22, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28328081/btk-inhibition-is-a-potent-approach-to-block-ige-mediated-histamine-release-in-human-basophils
#10
D Smiljkovic, K Blatt, G Stefanzl, Y Dorofeeva, C Skrabs, M Focke-Tejkl, W R Sperr, U Jaeger, R Valenta, P Valent
BACKGROUND: Recent data suggest that Bruton's tyrosine kinase (BTK) is an emerging therapeutic target in IgE receptor (IgER)-cross-linked basophils. METHODS: We examined the effects of four BTK inhibitors (ibrutinib, dasatinib, AVL-292, and CNX-774) on IgE-dependent activation and histamine release in blood basophils obtained from allergic patients (n=11) and nonallergic donors (n=5). In addition, we examined the effects of these drugs on the growth of the human basophil cell line KU812 and the human mast cell line HMC-1...
March 22, 2017: Allergy
https://www.readbyqxmd.com/read/28281330/basophil-activation-test-implementation-and-standardization-between-systems-and-between-instruments
#11
Anne-Emmanuelle Depince-Berger, Khaled Sidi-Yahya, Mohammed Jeraiby, Claude Lambert
The basophil activation test (BAT) is a good ex vivo alternative for measuring hypersensitivity to an allergen in sensitized patients but still lacks standardization. In this present study, we have implemented one of the systems and proposed inter-systems, inter-instrument standardization. Our method for basophil activation and labeling on whole blood: EDTA in one step using BasoflowEx(®) and FlowCast(®) . Setup on Navios and fluorescence targets converted to set up FACSCanto™ instrument. Our results: 1) A CD203c/CD63 (BasoflowEx) method was adapted for EDTA samples and simplified...
March 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28258478/in-vitro-diagnosis-of-immediate-drug-hypersensitivity-anno-2017-potentials-and-limitations
#12
REVIEW
I I Decuyper, E A Mangodt, A L Van Gasse, K Claesen, A Uyttebroek, M Faber, V Sabato, C H Bridts, C Mertens, M M Hagendorens, L S De Clerck, Didier G Ebo
BACKGROUND: For most physicians, quantification of drug-specific immunoglobulin E (drug-sIgE) antibodies constitutes the primary in vitro measure to document immediate drug hypersensitivity reactions (IDHR). Unfortunately, this is often insufficient to correctly identify patients with IgE-mediated IDHR and impossible for non-IgE-mediated IDHR that result from alternative routes of basophil and mast cell activation. In these difficult cases, diagnosis might benefit from cellular tests such as basophil activation tests (BAT)...
June 2017: Drugs in R&D
https://www.readbyqxmd.com/read/28238741/microparticles-in-nasal-lavage-fluids-in-chronic-rhinosinusitis-potential-biomarkers-for-diagnosis-of-aspirin-exacerbated-respiratory-disease
#13
Toru Takahashi, Atsushi Kato, Sergejs Berdnikovs, Whitney W Stevens, Lydia A Suh, James E Norton, Roderick G Carter, Kathleen E Harris, Anju T Peters, Kathryn E Hulse, Leslie C Grammer, Kevin C Welch, Stephanie Shintani-Smith, Bruce K Tan, David B Conley, Robert C Kern, Bruce S Bochner, Robert P Schleimer
BACKGROUND: Microparticles (MPs) are submicron-sized shed membrane vesicles released from activated or injured cells and are detectable by flow cytometry. MP levels have been used as biomarkers to evaluate cell injury or activation in patients with pathological conditions. OBJECTIVE: We sought to compare MP types and levels in nasal lavage fluids (NLFs) from controls and patients with chronic rhinosinusitis without nasal polyps (CRSsNP), chronic rhinosinusitis with nasal polyps (CRSwNP), and aspirin-exacerbated respiratory disease (AERD)...
February 24, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28219062/the-impact-on-allergy-related-cells-of-a-birch-pollen-allergoid-with-and-without-monophosphoryl-lipid-a-in-comparison-with-the-native-equivalent
#14
Margitta Worm, Dennis Ernst, Markus Kraller, Magda Babina
BACKGROUND: The clinical efficacy and safety of allergoid immunotherapy have been demonstrated in clinical trials. However, simultaneous monitoring of the immunological changes by allergoids versus allergens in the cells of the same individual has not been extensively performed, and the impact of concurrent Toll-like receptor 4 (TLR4) ligation has not been specified. METHODS: Three types of birch allergen were utilized: glutaraldehyde-treated allergoid (extract A), the same allergoid plus monophosphoryl lipid A (MPL), i...
2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/28034549/basophil-activation-test-is-a-relevant-biomarker-of-the-outcome-of-rapid-desensitization-in-platinum-compounds-allergy
#15
Pedro Giavina-Bianchi, Violeta Régnier Galvão, Matthieu Picard, Joana Caiado, Mariana C Castells
BACKGROUND: Rapid drug desensitization (RDD) has become a cornerstone in the management of immediate drug hypersensitivity reactions (DHRs) to chemotherapeutic agents. Because of the inherent risk of anaphylaxis during RDD, biomarkers to predict patients at risk of developing such severe reactions are needed. The basophil activation test (BAT) has been used in DHRs as a diagnostic tool. OBJECTIVE: We evaluated basophil CD63 and CD203c expression (BAT) as a biomarker to assess the safety and effectiveness of RDD in platinum compounds-allergic patients...
May 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28000952/human-basophils-are-a-source-of-and-are-differentially-activated-by-il-31
#16
U Raap, M Gehring, S Kleiner, U Rüdrich, B Eiz-Vesper, H Haas, A Kapp, B F Gibbs
BACKGROUND: Basophils are important effector cells involved in the pathogenesis of inflammatory skin diseases including chronic urticaria which is associated by increased IL-31 serum levels. So far the effects of IL-31 on human basophils are unknown. OBJECTIVE: To analyse the functional role of IL-31 in basophil biology. METHODS: IL-31 expression was evaluated in skin samples derived from chronic spontaneous urticaria patients. Oncostatin M receptor (OSMR), IL-31 receptor A (RA) and IL-31 protein expressions were analysed on human basophils from healthy donors...
April 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27999658/exploratory-analysis-of-cd63-and-cd203c-expression-in-basophils-from-hazelnut-sensitized-and-allergic-individuals
#17
Bianca Lötzsch, Sabine Dölle, Stefan Vieths, Margitta Worm
BACKGROUND: Sensitization to hazelnut (HN) is frequent and requires clarification to determine whether this sensitization is clinically relevant. The aim of this study was to investigate basophil activation profiles in HN-sensitized and allergic subjects. METHODS: Basophil activation was determined by flow cytometric analyses of CD63 and CD203c expression using several HN allergen concentrations. Depending on their clinical reaction pattern, an oral allergy symptom group (OAS, n = 20), a systemic reaction group (n = 12) and a sensitized group without clinical symptoms (n = 20) were identified...
2016: Clinical and Translational Allergy
https://www.readbyqxmd.com/read/27582406/the-basophil-activation-test-is-safe-and-useful-for-confirming-drug-induced-anaphylaxis
#18
Suk Yeon Kim, Joo Hee Kim, Young Sook Jang, Jeong Hee Choi, Sunghoon Park, Yong Il Hwang, Seung Hun Jang, Ki Suck Jung
The basophil activation test (BAT) has been suggested as a complementary method for diagnosing drug allergies. The aim of this study was to evaluate the clinical utility of this test in patients with drug-induced anaphylaxis. In total, 19 patients, all of whom had a history of moderate to severe anaphylaxis, were enrolled. None of the causative drugs had available in vitro tests or reliable skin tests; these drugs included, among others, first and second-generation cephalosporins, H2 blockers, and muscle relaxants...
November 2016: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27533953/effect-of-rapid-desensitization-on-platelet-inhibition-and-basophil-activation-in-patients-with-aspirin-hypersensitivity-and-coronary-disease
#19
Stéphane Manzo-Silberman, Pascale Nicaise-Roland, Catherine Neukirch, Florence Tubach, Marie-Geneviève Huisse, Sylvie Chollet-Martin, Hélène Abergel, Fathi Driss, Toni Alfaiate, Nadine Ajzenberg, Philippe Gabriel Steg
Aims: To determine antiplatelet efficacy after desensitization in patients with a history of aspirin hypersensitivity. Methods and results: We conducted a case-control study to evaluate the efficacy of aspirin 1 day (D1) and 6-8 weeks (W6-8) after desensitization. We also assessed ex vivo basophil reactivity to aspirin after desensitization. Cases were patients with coronary artery disease (CAD) and documented history of aspirin hypersensitivity who underwent rapid successful oral desensitization to aspirin...
April 1, 2017: European Heart Journal. Cardiovascular Pharmacotherapy
https://www.readbyqxmd.com/read/27527263/assessing-basophil-activation-by-using-flow-cytometry-and-mass-cytometry-in-blood-stored-24%C3%A2-hours-before-analysis
#20
Kaori Mukai, Nicolas Gaudenzio, Sheena Gupta, Nora Vivanco, Sean C Bendall, Holden T Maecker, Rebecca S Chinthrajah, Mindy Tsai, Kari C Nadeau, Stephen J Galli
BACKGROUND: Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies. OBJECTIVE: We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample. METHODS: Blood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs...
March 2017: Journal of Allergy and Clinical Immunology
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