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Suk Yeon Kim, Joo Hee Kim, Young Sook Jang, Jeong Hee Choi, Sunghoon Park, Yong Il Hwang, Seung Hun Jang, Ki Suck Jung
The basophil activation test (BAT) has been suggested as a complementary method for diagnosing drug allergies. The aim of this study was to evaluate the clinical utility of this test in patients with drug-induced anaphylaxis. In total, 19 patients, all of whom had a history of moderate to severe anaphylaxis, were enrolled. None of the causative drugs had available in vitro tests or reliable skin tests; these drugs included, among others, first and second-generation cephalosporins, H2 blockers, and muscle relaxants...
November 2016: Allergy, Asthma & Immunology Research
Stéphane Manzo-Silberman, Pascale Nicaise-Roland, Catherine Neukirch, Florence Tubach, Marie-Geneviève Huisse, Sylvie Chollet-Martin, Hélène Abergel, Fathi Driss, Toni Alfaiate, Nadine Ajzenberg, Philippe Gabriel Steg
AIMS: To determine antiplatelet efficacy after desensitization in patients with a history of aspirin hypersensitivity. METHODS AND RESULTS: We conducted a case-control study to evaluate the efficacy of aspirin 1 day (D1) and 6-8 weeks (W6-8) after desensitization. We also assessed ex vivo basophil reactivity to aspirin after desensitization. Cases were patients with coronary artery disease (CAD) and documented history of aspirin hypersensitivity who underwent rapid successful oral desensitization to aspirin...
August 5, 2016: European Heart Journal. Cardiovascular Pharmacotherapy
Kaori Mukai, Nicolas Gaudenzio, Sheena Gupta, Nora Vivanco, Sean C Bendall, Holden T Maecker, Rebecca S Chinthrajah, Mindy Tsai, Kari C Nadeau, Stephen J Galli
BACKGROUND: Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies. OBJECTIVE: We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample. METHODS: Blood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs...
July 15, 2016: Journal of Allergy and Clinical Immunology
Arnold S Kirshenbaum, Glenn Cruse, Avanti Desai, Geethani Bandara, Maarten Leerkes, Chyi-Chia R Lee, Elizabeth R Fischer, Kevin J O'Brien, Bernadette R Gochuico, Kelly Stone, William A Gahl, Dean D Metcalfe
Hermansky-Pudlak Syndrome type-1 (HPS-1) is an autosomal recessive disorder caused by mutations in HPS1 which result in reduced expression of the HPS-1 protein, defective lysosome-related organelle (LRO) transport and absence of platelet delta granules. Patients with HPS-1 exhibit oculocutaneous albinism, colitis, bleeding and pulmonary fibrosis postulated to result from a dysregulated immune response. The effect of the HPS1 mutation on human mast cells (HuMCs) is unknown. Since HuMC granules classify as LROs along with platelet granules and melanosomes, we set out to determine if HPS-1 cutaneous and CD34+ culture-derived HuMCs have distinct granular and cellular characteristics...
2016: PloS One
N Cop, I I Decuyper, M A Faber, V Sabato, C H Bridts, M M Hagendorens, B Y De Winter, L S De Clerck, D G Ebo
BACKGROUND: Mast cell progenitor cells, derived from CD34(+) hematopoietic stem cells, enter the circulation and subsequently mucosal or connective tissues where they mature to mast cells. Upon activation, mast cells increase the expression of activation markers, e.g. CD63, and release histamine amongst other mediators. Traditionally, release of these mediators is quantified using assays measuring their extracellular concentration in the supernatant of stimulated cells. METHODS: Human mast cells were cultured from peripheral blood, phenotypically characterized, passively sensitized with allogenic IgE antibodies and finally stimulated by anti-IgE that cross-links IgE/FcεRI complexes...
July 12, 2016: Cytometry. Part B, Clinical Cytometry
Hiroyuki Shiono, Takuya Matsui, Tadashi Okada, Yoichiro Ito
We have developed a novel continuous flow-through cell separation method using a Percoll density gradient. This method can continuously separate a large number of cells into five fractions according to their densities. To apply this method to the separation of basophils, Percoll density gradients were modified to improve basophil enrichment. When a set of Percoll density gradients was prepared (1.071, 1.075, 1.080, 1.084, and 1.090 g/mL) the basophils in a healthy volunteer were enriched by an average of 23...
August 2016: Journal of Separation Science
Tahia D Fernández, Adriana Ariza, Francisca Palomares, María I Montañez, María Salas, Angela Martín-Serrano, Rubén Fernández, Arturo Ruiz, Miguel Blanca, Cristobalina Mayorga, María J Torres
Although fluoroquinolones (FQs) are generally well-tolerated antibiotics, increasing numbers of hypersensitivity reactions have been reported. These can be evaluated in vitro by basophil activation tests (BATs); however, sensitivity is not optimal. Many factors could influence sensitivity such as basophil activation markers. The objective of this study was to evaluate the influence of 2 different activations markers, CD63 and CD203c, on the sensitivity of BAT to FQ. We studied 17 patients with immediate allergic reactions to FQ...
June 2016: Medicine (Baltimore)
Yael Gernez, Jeffrey Waters, Rabindra Tirouvanziam, Leonore Herzenberg, Richard Moss
No abstract text is available yet for this article.
April 22, 2016: Journal of Allergy and Clinical Immunology
S Kleiner, G-J Braunstahl, U Rüdrich, M Gehring, B Eiz-Vesper, T A Luger, B Steelant, S F Seys, A Kapp, M Böhm, P W Hellings, U Raap
BACKGROUND: α-melanocyte-stimulating hormone (α-MSH) was shown to inhibit allergic airway inflammation and exert suppressive effects on human basophils. OBJECTIVE: This study aims to extend our current knowledge on the melanocortin 1 receptor (MC1R) expression in nasal tissue of patients with allergic rhinitis (AR) and functional effects of α-MSH in human basophils especially from patients with allergic rhinitis. METHODS: MC1R expression before and after nasal allergen provocation was studied in nasal mucosal tissue of AR patients and in a mouse model of allergic airway inflammation using immunofluorescence...
August 2016: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Takuya Iwamoto, Hiroko Sugimoto, Tsutomu Tabata, Masahiro Okuda
PURPOSE: Drug desensitization has been found to be an effective option for carboplatin rechallenge in patients at risk for severe hypersensitivity reaction. However, identifying such patients requires precise clinical tests. This study was performed to evaluate the clinical utility of basophil CD203c to predict the timing of carboplatin-induced severe hypersensitivity reaction. METHODS: Here we report on 3 patients undergoing a carboplatin-desensitization protocol at Mie University Hospital...
June 2016: Clinical Therapeutics
N Harada, M Hiragun, M Mizuno, T Kawaguchi, K Ishii, Y Yanase, T Hiragun, Y Sugita, M Hide
No abstract text is available yet for this article.
2016: Journal of Investigational Allergology & Clinical Immunology
Shih Han Tsai, Kiyoshi Takeda
Adenosine 5'-triphosphate (ATP) is released from dying or damaged cells, as well as from activated cells. Once secreted, extracellular ATP induces several immune responses via P2X and P2Y receptors. Basophils and mast cells release ATP upon FcεRI-crosslinking, and ATP activates basophils and mast cells in an autocrine manner. Nucleotide-converting ectoenzymes, such as E-NTPD1, E-NTPD7, and E-NPP3, inhibit ATP-dependent immune responses by hydrolyzing ATP, thereby contributing to immune response regulation...
September 2016: Seminars in Immunopathology
Stavroula Giavi, Yvonne M Vissers, Antonella Muraro, Roger Lauener, Anastasios P Konstantinopoulos, Annick Mercenier, Antoine Wermeille, Francesca Lazzarotto, Remo Frei, Roberta Bonaguro, Selina Summermatter, Sophie Nutten, Nikolaos G Papadopoulos
BACKGROUND: A major drawback of oral immunotherapy for food allergy, is the possibility of severe side effects. We assessed both safety and efficacy of a low allergenic hydrolysed egg (HydE) preparation used in a double-blind placebo controlled randomized study in egg allergic children. METHODS: In a pilot multicentre study, 29 egg allergic patients (aged 1-5.5 years) were administered daily for 6 months 9g HydE or placebo in a blinded, randomised manner. Safety was verified by oral food challenge to assess tolerance towards HydE at the start, and efficacy by an open oral food challenge (OFC, primary outcome) at the end...
April 5, 2016: Allergy
Alexandra F Santos, Natalia Bécares, Alick Stephens, Victor Turcanu, Gideon Lack
BACKGROUND: Basophil activation test (BAT) reproduces IgE-mediated allergic reactions in vitro and has been used as a diagnostic test. Different markers can be used to identify basophils in whole blood and have implications for the outcome of the test. We aimed to assess changes in the expression of CD123 and HLA-DR following basophil activation and to select the best gating strategy for BAT using these markers. METHODS: BAT was performed in whole blood from 116 children...
2016: Clinical and Translational Allergy
Masooma Abdullahi, Reza Ranjbaran, Soheaila Alyasin, Zeinab Keshavarz, Amin Ramezani, Abbas Behzad-Behbahani, Sedigheh Sharifzadeh
BACKGROUND: Asthma is very common in children and its diagnosis is based on clinical manifestations, which can be misdiagnosed as other respiratory diseases with similar signs and symptoms. OBJECTIVE: To analyze the expression of ST2L and CD203c in the diagnosis of pediatric asthma. METHODS: Basophils were purified from whole blood samples of patients and healthy controls using Ficol-Paque gradient and Basophil Isolation Kit. RNA extraction was done by RNX-Plus solution and after synthesis of cDNA, the gene expression was analyzed by means of real time PCR...
March 2016: Iranian Journal of Immunology: IJI
S Mommert, S Kleiner, M Gehring, B Eiz-Vesper, H Stark, R Gutzmer, T Werfel, U Raap
BACKGROUND: IgE-mediated cross-linking of FcεRI results in the release of mediators stored in basophil granules, such as histamine and proteases, and in the de novo synthesis of sulfidoleukotrienes. OBJECTIVE: In this study, we investigated the role of the histamine receptors, in particular that of the histamine H4 receptor (H4R), in modulating human basophil function. METHODS: The mRNA expression of the histamine receptors was measured by real-time PCR...
September 2016: Allergy
Sadan Soyyigit, Deniz Guloglu, Aydan Ikinciogullari, Derya Secil, Derya Oztuna, Dilsad Mungan, Zeynep Misirligil, Betul Ayse Sin
BACKGROUND: There is a continuing debate about whether monoallergen subcutaneous immunotherapy (SCIT) is able to modulate immune and clinical responses toward main causal allergen in polysensitized patients. OBJECTIVE: To investigate short-term immunologic changes and clinical effectiveness of SCIT with Dermatophagoides pteronyssinus in monosensitized and polysensitized patients who have rhinitis with or without asthma. METHODS: Nineteen monosensitized and 24 polysensitized patients participated in this prospective, self-placebo-controlled, interventional study...
March 2016: Annals of Allergy, Asthma & Immunology
Anna Katelari, Marianna Tzanoudaki, Maria Noni, Maria Kanariou, Maria Theodoridou, Emmanuel Kanavakis, Stavros-Eleftherios Doudounakis, Christina Kanaka-Gantenbein
BACKGROUND: Allergic bronchopulmonary aspergillosis (ABPA) and Aspergillus fumigatus sensitization (AFS) are quite often observed in cystic fibrosis (CF) patients. The aim of this study was to evaluate the use of basophil activation test (BAT) in these manifestations of hypersensitivity reactions. METHODS: BAT (CD63 and CD203c) was performed for 56 CF patients (17 ABPA, 24 AFS and 15 non-AFS). A receiver operating characteristic (ROC) curve analysis and a survival analysis were performed...
September 2016: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
Tomoaki Kumagai, Nozomi Yamaguchi, Hiroyuki Hirai, Shigeyuki Kojima, Yoshiko Kodani, Akihiko Hashiguchi, Michiko Haida, Masataka Nakamura
Basophil activation test (BAT) is an in vitro allergy test that is useful to identify allergens that cause IgE-dependent allergies. The test has been used to detect not only food allergies and allergies caused by environmental factors but also to detect drug hypersensitivity, which has been known to include IgE-independent reactions. In our preliminary studies in which BAT was applied to detect hypersensitivity of loxoprofen, a non-steroidal anti-inflammatory drug (NSAID), conventional BAT with incubation for 30min did not show basophil activation by means of increased CD203c expression...
April 2016: International Immunopharmacology
Thamiris Palacios, Leland Stillman, Larry Borish, Monica Lawrence
No abstract text is available yet for this article.
May 2016: Journal of Allergy and Clinical Immunology in Practice
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