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https://www.readbyqxmd.com/read/28944996/negative-pressure-wound-therapy-promotes-muscle-derived-stem-cell-osteogenic-differentiation-through-mapk-pathway
#1
Hong Liu, Xun Zheng, Liang Chen, Chao Jian, Xiang Hu, Yong Zhao, Zonghuan Li, Aixi Yu
Negative pressure wound therapy (NPWT) has been revealed to be effective in the treatment of open fractures, although the underlying mechanism is not clear. This article aimed to investigate the effects of NPWT on muscle-derived stem cell (MDSC) osteoblastic differentiation and the related potential mechanism. The cell proliferation rate was substantially increased in NPWT-treated MDSCs in comparison with a static group for 3 days. There was no observable effect on the apoptosis of MDSC treated with NPWT compared with the control group for 3 days...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944927/%C3%AE-7-nicotinic-acetylcholine-receptor-agonist-attenuates-the-cerebral-injury-in-a-rat-model-of-cardiopulmonary-bypass-by-activating-the-akt-gsk3%C3%AE-pathway
#2
Yingjie Sun, Dandan Song, Mei Wang, Keyan Chen, Tiezheng Zhang
α7 nicotinic acetylcholine receptor (α7nAchR) agonist treatment may provide a promising therapeutic effect for cerebral injuries. However, it is unclear whether the activation of α7nAchR agonist may reduce cerebral injuries induced by cardiopulmonary bypass (CPB). A total of 96 male Sprague‑Dawley rats were randomly divided into four groups (n=24/group): i) Sham operation group; ii) CPB group; iii) CPB + α7nAchR agonist group; and iv) CPB + α7nAchR agonist + α7nAchR antagonist group. Following treatment, 24 rats from each group were sacrificed and the serum and hippocampal tissues were collected...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944924/cyclic-helix-b-peptide-protects-hk%C3%A2-2-cells-from-oxidative-stress-by-inhibiting-er-stress-and-activating-nrf2-signalling-and-autophagy
#3
Long Li, Miao Lin, Lexi Zhang, Shang Huang, Chao Hu, Long Zheng, Liping Li, Chao Zhang, Cheng Yang, Yaqiu Long, Ruiming Rong, Tongyu Zhu
Renal ischemia‑reperfusion injury (IRI) is present in numerous diseases and is observed following certain treatments, including renal transplantation. Preventing tubular epithelial cells (TECs) from undergoing apoptosis is vital for treatment of renal IRI. Cyclic helix B peptide (CHBP) is a novel agent that has a protective effect on renal IRI in vivo. In the present study, the effect and underlying mechanism of CHBP on TECs was investigated. The HK‑2 human renal proximal tubular epithelial cell line was treated with 500 µmol/l H2O2 for 4 h prior to determining the effect of CHBP pretreatment for 1 h on cell viability, caspase 3 activity and expression levels, expression levels of oxidative stress markers, endoplasmic reticulum (ER) stress markers, NF‑E2‑related factor 2 (Nrf2), heme oxygenase‑1 (HO‑1) and autophagy markers...
September 22, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944915/neuroprotective-effect-of-total-flavonoids-from-ilex-pubescens-against-focal-cerebral-ischemia-reperfusion-injury-in-rats
#4
Xiaoyan Fang, Yujie Li, Jingyi Qiao, Ying Guo, Mingsan Miao
Ilex pubescens is commonly used in traditional Chinese medicine to treat cardiovascular and cerebrovascular diseases, such as coronary artery disease and stroke. However, the underlying mechanisms remain to be fully elucidated. The aim of the present study was to investigate the effects of Ilex pubescens total flavonoids (IPTF) on neuroprotection and the potential mechanisms in a rat model of focal cerebral ischemia/reperfusion (I/R) injury. Rats were pretreated with intragastric administration of IPTF at doses of 200 and 100 mg/kg for 5 days; middle cerebral artery occlusion surgery was then performed to induce cerebral I/R injury...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944867/succinate-promotes-skeletal-muscle-protein-synthesis-via-erk1-2-signaling-pathway
#5
Yexian Yuan, Yaqiong Xu, Jingren Xu, Bingqing Liang, Xingcai Cai, Canjun Zhu, Lina Wang, Songbo Wang, Xiaotong Zhu, Ping Gao, Xiuqi Wang, Yongliang Zhang, Qingyan Jiang, Gang Shu
It is well known that endurance training is effective to attenuate skeletal muscle atrophy. Succinate is a typical TCA metabolite, of which exercise could dramatically increase the content. The present study aimed to investigate the effect of succinate on protein synthesis in skeletal muscle, and try to delineate the underlying mechanism. The in vitro study revealed that succinate dose‑dependently increased protein synthesis in C2C12 myotube along with the enhancement of phosphorylation levels of AKT Serine/Threonine Kinase 1(Akt), mammalian target of rapamycin, S6, eukaryotic translation initiation factor 4E, 4E binding protein 1 and forkhead box O (FoxO) 3a...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944842/mitochondrial-dna%C3%A2-induced-inflammatory-damage-contributes-to-myocardial-ischemia-reperfusion-injury-in-rats-cardioprotective-role-of-epigallocatechin
#6
Chao-Yi Qin, Hong-Wei Zhang, Jun Gu, Fei Xu, Huai-Min Liang, Kang-Jun Fan, Jia-Yu Shen, Zheng-Hua Xiao, Er-Yong Zhang, Jia Hu
Inflammation serves an important role in the pathogenesis of myocardial ischemia/reperfusion (I/R) injury. Fragments of endogenous damaged‑associated molecular patterns, recently identified as mitochondrial DNA (mtDNA), have been proven to be a potent pro‑inflammatory mediator. Epigallocatechin‑3‑gallate (EGCG) is able to regulate the expression levels of a series of inflammatory cytokines. However, the involvement of endogenous mtDNA in EGCG‑regulated inflammatory activities in the context of myocardial I/R injury remains to be elucidated...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944840/correlation-analysis-between-liver-metastasis-and-serum-levels-of-mir%C3%A2-200-and-mir%C3%A2-141-in-patients-with-colorectal-cancer
#7
Meng Ding, Tao Zhang, Shijie Li, Ying Zhang, Yunfeng Qiu, Bin Zhang
Colorectal cancer has become a disease with one of the highest incidence rates worldwide, and liver metastasis threatens the prognosis of colorectal cancer. MicroRNA (miR)‑200 and miR‑141 have been confirmed to be aberrantly expressed in colorectal cancer, however, whether miR‑200 and miR‑141 are involved in the progression of colorectal cancer, and their role in liver metastasis remain to be fully elucidated. The aim of the present study was to investigate the role of miR‑200 and miR‑141 in the progression and liver metastasis of colorectal cancer in patients...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944833/low-doses-of-co-nanoparticles-induce-death-and-regulate-osteogenic-differentiation-in-mg%C3%A2-63-cells
#8
Qinglin Han, Fan Liu
The aim of the present study was to investigate the effects of cobalt nanoparticles (CoNPs) on the proliferation and differentiation of human osteoblasts in vitro, and to investigate the molecular mechanisms via which CoNPs affect proliferation and differentiation of osteoblasts. The MG‑63 human osteoblast cell line was treated with different concentrations of CoNPs for 12 to 48 h in vitro. At each time point, cell morphology was observed and an MTT assay was performed to assess cell viability. Alkaline phosphatase (ALP), osteocalcin (BGLAP), collagen I (COL I), osteoprotegerin (OPG) and receptor activator of nuclear factor κ‑B ligand mRNA expression levels, and ALP, BGLAP and COL protein expression levels, were assessed by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944827/cerebral-ischemia-reperfusion-injury-induces-airway-mucus-hypersecretion-in-rats-and-activates-il%C3%A2-13%C3%A2-associated-inflammatory-mechanisms
#9
Baoning Xu, Lei Zhang, Yuqin Che, Chengyang Song, Wenjun Jiang, Jianhua Fan, Dali Tian
The majority of patients that suffer a stroke have excessive sputum, which accelerates the development of pulmonary complications. However, it is unclear whether cerebral ischemia and reperfusion (I/R) injury induces mucus hypersecretion, and the potential role of inflammation remains unknown. In the present study, the reversible middle cerebral artery occlusion model was applied in rats to induce cerebral I/R injury. The rats were grouped according to the duration of reperfusion (6, 12, 24, 48 and 72 h). Neurological dysfunction was evaluated by Longa scoring and lung dry‑to‑wet weight (dw/ww) ratios were determined to reflect the degree of mucus secretion...
September 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944807/conjugated-fatty-acid-rich-oil-from-gynostrmma-pentaphyllum-seed-can-ameliorate-lipid-and-glucose-metabolism-in-type-2-diabetes-mellitus-mice
#10
Mingxing Zhang, Nan Du, Lu Wang, Xiaobing Wang, Yaping Xiao, Kun Zhang, Quanhong Liu, Pan Wang
Gynostrmma pentaphyllum seed oil (GPSO), extracted from G. pentaphyllum seeds, is rich in conjugated linolenic acid, which is a special fatty acid consisting of cis-9, trans-11, trans-13 isomers. Type 2 diabetes mellitus (T2DM) is characterized by hyperglycemia resulting from insulin resistance, and is usually accompanied by hypertension, hyperlipidemia, atherosclerosis (i.e., the metabolic syndrome, or syndrome X), and polycystic ovarian disease. This study aimed to investigate the effect of GPSO on T2DM hepatic lipid metabolism and the underlying mechanism involving level of protein expression...
September 25, 2017: Food & Function
https://www.readbyqxmd.com/read/28944697/influence-of-trunk-control-and-lower-extremity-impairments-on-gait-capacity-in-children-with-cerebral-palsy
#11
Julia Balzer, Petra Marsico, Elena Mitteregger, Marietta L van der Linden, Thomas H Mercer, Hubertus J A van Hedel
PURPOSE: We investigated the combined impact of trunk control and lower extremities impairments on predicting gait capacity in children with cerebral palsy (CP) and evaluated relationships between trunk control and lower extremities impairments. METHODS: Data of 52 children with CP [29 boys, mean age 11 years 9 months (±4 years 6 months)] were included in this observational study. Gait capacity was measured by the "modified Time Up and Go test". Experienced therapists performed the "Modified Ashworth Scale", "Manual Muscle Test", the "Selective Control Assessment of the Lower Extremity", and the "Trunk Control Measurement Scale"...
September 24, 2017: Disability and Rehabilitation
https://www.readbyqxmd.com/read/28944676/multiphoton-absorption-order-of-cspbbr3-as-determined-by-wavelength-dependent-nonlinear-optical-spectroscopy
#12
Felix Ochieng Saouma, Constantinos C Stoumpos, Mercouri G Kanatzidis, Yong Soo Kim, Joon I Jang
CsPbBr3 is a direct-gap semiconductor where optical absorption takes place across the fundamental bandgap. But this all-inorganic halide perovskite typically exhibits above-bandgap emission when excited over an energy level, lying above the conduction-band minimum. We probe this bandgap anomaly using wavelength-dependent multiphoton absorption spectroscopy and find that the fundamental gap is strictly two-photon forbidden, rendering it three-photon absorption (3PA) active. Instead, two-photon absorption (2PA) commences when the two-photon energy is resonant with the optical gap, associated with the level causing the anomaly...
September 25, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28944589/evolution-of-gene-expression-after-whole-genome-duplication-new-insights-from-the-spotted-gar-genome
#13
Jeremy Pasquier, Ingo Braasch, Peter Batzel, Cedric Cabau, Jérome Montfort, Thaovi Nguyen, Elodie Jouanno, Camille Berthelot, Christophe Klopp, Laurent Journot, John H Postlethwait, Yann Guiguen, Julien Bobe
Whole-genome duplications (WGDs) are important evolutionary events. Our understanding of underlying mechanisms, including the evolution of duplicated genes after WGD, however, remains incomplete. Teleost fish experienced a common WGD (teleost-specific genome duplication, or TGD) followed by a dramatic adaptive radiation leading to more than half of all vertebrate species. The analysis of gene expression patterns following TGD at the genome level has been limited by the lack of suitable genomic resources. The recent concomitant release of the genome sequence of spotted gar (a representative of holosteans, the closest-related lineage of teleosts that lacks the TGD) and the tissue-specific gene expression repertoires of over 20 holostean and teleostean fish species, including spotted gar, zebrafish, and medaka (the PhyloFish project), offers a unique opportunity to study the evolution of gene expression following TGD in teleosts...
September 25, 2017: Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
https://www.readbyqxmd.com/read/28944583/venous-anastomosis-using-a-nonpenetrating-vascular-closure-system-in-orthotopic-liver-transplantation
#14
Yuan Wang, Zhao Xin, Bing Pan, Shao-Cheng Lv, Xing-Mao Zhang, Zhi-Hua Zhang, Li-Xin Li, Xian-Liang Li, Qiang He
BACKGROUND: A nonpenetrating vessel closure system (VCS-AnastoClip(®) ) may facilitate vascular anastomosis. The purpose of this study is to explore the utilization of a nonpenetrating VCS in orthotopic liver transplantation (OLT). METHODS: From January 2015 to February 2017, patients who underwent OLT were divided into two groups, i.e., those who underwent nonpenetrating VCS application for inferior vena cava (IVC) and portal vein (PV) reconstructions and those who underwent hand sewing for these purposes...
September 25, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28944557/enhanced-sensitivity-and-responses-to-viologens-from-a-whole-cell-bacterial-bioreporter-treated-with-branched-polyethyleneimines
#15
Sandrine Soh Mabekou, Sang Cheon Lee, Thu Huong Dinh, Keehoon Won, Robert J Mitchell
AIMS: Evaluate the use of polyethyleneimines (PEIs) as membrane permeabilizers to improve the responses and sensitivity of a bacterial bioreporter strain to viologens. METHODS AND RESULTS: The responses from E. coli str. EBS, i.e., E. coli BW25113 carrying plasmid pSDS, when exposed to five different viologens was characterized, as were the toxicities of seven different PEIS, including two linear and five branched species. Based upon these results, benzyl viologen led to the greatest responses and 0...
September 25, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28944530/effect-of-changing-forage-on-the-dynamic-variation-in-rumen-fermentation-in-sheep
#16
Xiao Xie, Jia-Kun Wang, Leluo Guan, Jian-Xin Liu
To better understand rumen adaptation during dietary transitions between high- and low-quality forages, 10 rumen-cannulated Hu sheep were randomly allocated to two dietary treatments (five sheep each) with the same concentrate-to-forage ratio and concentration mixture, but different forage sequences: (i) alfalfa hay (AH) to corn stover (CS) and back to AH; and (ii) CS to AH and back to CS. A significant decrease in the rumen microbial protein concentration was observed on day 6 after dietary transition whether the transition was from AH to CS or from CS to AH, and this was accompanied by an increase in the ammonia nitrogen concentration as well as a decrease in the total volatile fatty acids concentration and pH...
September 25, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28944518/scenarios-reveal-pathways-to-sustain-future-ecosystem-services-in-an-agricultural-landscape
#17
Jiangxiao Qiu, Stephen R Carpenter, Eric G Booth, Melissa Motew, Samuel C Zipper, Christopher J Kucharik, Xi Chen, Steven P Loheide, Jenny Seifert, Monica G Turner
Sustaining food production, water quality, soil retention, flood and climate regulation in agricultural landscapes is a pressing global challenge given accelerating environmental changes. Scenarios are stories about plausible futures, and scenarios can be integrated with biophysical simulation models to explore quantitatively how the future might unfold. However, few studies have incorporated a wide range of drivers (e.g., climate, land-use, management, population, human diet) in spatially explicit, process-based models to investigate spatial-temporal dynamics and relationships of a portfolio of ecosystem services...
September 25, 2017: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/28944511/cost-utility-analysis-of-direct-vad-versus-double-bridges-to-heart-transplantation-in-patients-with-refractory-heart-failure
#18
Hsiao-Huang Chang, Po-Lin Chen, I-Ming Chen, Tzu-Ting Kuo, Zen-Chung Weng, Pei-Jung Huang, Nai-Yuan Wu, Ching-Li Cheng
OBJECT: This study compared the cost-utility of direct ventricular assist device (VAD) versus double bridges, extracorporeal membrane oxygenation (ECMO) before VAD, to heart transplantation in patients with refractory heart failure. MATERIALS AND METHODS: From a health payer perspective, a Markov model was developed. The cycle length was one month and the time horizon was a lifetime. Probabilities and direct cost data were calculated from a nationwide claim database...
September 25, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28944453/personalised-digital-interventions-for-reducing-hazardous-and-harmful-alcohol-consumption-in-community-dwelling-populations
#19
REVIEW
Eileen Fs Kaner, Fiona R Beyer, Claire Garnett, David Crane, Jamie Brown, Colin Muirhead, James Redmore, Amy O'Donnell, James J Newham, Frank de Vocht, Matthew Hickman, Heather Brown, Gregory Maniatopoulos, Susan Michie
BACKGROUND: Excessive alcohol use contributes significantly to physical and psychological illness, injury and death, and a wide array of social harm in all age groups. A proven strategy for reducing excessive alcohol consumption levels is to offer a brief conversation-based intervention in primary care settings, but more recent technological innovations have enabled people to interact directly via computer, mobile device or smartphone with digital interventions designed to address problem alcohol consumption...
September 25, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28944423/differential-dna-hydroxymethylation-in-human-uterine-leiomyoma-cells-depending-on-the-phase-of-menstrual-cycle-and-presence-of-med12-gene-mutations
#20
A S Kol'tsova, A A Pendina, O A Efimova, A N Kaminskaya, A V Tikhonov, N S Osinovskaya, I Yu Sultanov, N Yu Shved, M I Kakhiani, V S Baranov
Using immunofluorescence with specific antibodies, we analyzed DNA hydroxymethylation in uncultured cells from 25 human uterine leiomyomas considering the menstrual cycle phase during surgery and the presence of MED12 gene mutations. It was found that each tumor node had specific DNA hydroxymethylation level that did not depend on the presence of mutations in MED12 gene, but depended on the phase of menstrual cycle. The degree of DNA hydroxymethylation was significantly lower in cells of leiomyomas excised during the luteal phase compared to the follicular phase (p=0...
September 25, 2017: Bulletin of Experimental Biology and Medicine
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