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https://www.readbyqxmd.com/read/28408361/design-of-a-mobile-app-for-nutrition-education-trec-lifestyle-and-formative-evaluation-with-families-of-overweight-children
#1
Silvia Gabrielli, Marco Dianti, Rosa Maimone, Marta Betta, Lorena Filippi, Monica Ghezzi, Stefano Forti
BACKGROUND: Nutrition and diet apps represent today a popular area of mobile health (mHealth), offering the possibility of delivering behavior change (BC) interventions for healthy eating and weight management in a scalable and cost-effective way. However, if commercial apps for pediatric weight management fail to retain users because of a lack of theoretical background and evidence-based content, mHealth apps that are more evidence-based are found less engaging and popular among consumers...
April 13, 2017: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/28381232/detection-of-newly-produced-t-and-b-lymphocytes-by-digital-pcr-in-blood-stored-dry-on-nylon-flocked-swabs
#2
Marion Vaglio Tessitore, Alessandra Sottini, Aldo M Roccaro, Claudia Ghidini, Simona Bernardi, Giovanni Martellosio, Federico Serana, Luisa Imberti
BACKGROUND: A normal number of T-cell receptor excision circles (TRECs) and K-deleting recombination excision circles (KRECs) is considered a biomarker for adequate new T- and B-cell production. In newborns, detection of TRECs and KRECs by real time PCR from dried blood spotted on filter paper is used for the screening of severe immunodeficiency. In adults, elderly and during diseases, where the number of TRECs is lower than in newborns and children, a large amount of DNA and a sensitive method of amplification are necessary to identify newly produced lymphocytes...
April 5, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28373873/novel-senescent-regulatory-t-cell-subset-with-impaired-suppressive-function-in-rheumatoid-arthritis
#3
Johannes Fessler, Andrea Raicht, Rusmir Husic, Anja Ficjan, Christine Schwarz, Christina Duftner, Wolfgang Schwinger, Winfried B Graninger, Martin H Stradner, Christian Dejaco
OBJECTIVE: Premature senescence of lymphocytes is a hallmark of inflammatory rheumatic diseases such as rheumatoid arthritis (RA). Early T-cell aging affects conventional T-cells but is presumably not limited to this cell population; rather it might also occur in the regulatory T-cells (Tregs) compartment. In RA, Tregs fail to halt aberrant immune reactions and disease progression. Whether this is associated with early Treg senescence leading to phenotypic and functional changes of this subset is elusive so far...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28229174/characterization-of-the-specific-interaction-between-the-dna-aptamer-sgc8c-and-protein-tyrosine-kinase-7-receptors-at-the-surface-of-t-cells-by-biosensing-afm
#4
Michael Leitner, Alexandra Poturnayova, Constanze Lamprecht, Sabine Weich, Maja Snejdarkova, Ivana Karpisova, Tibor Hianik, Andreas Ebner
We studied the interaction of the specific DNA aptamer sgc8c immobilized at the AFM tip with its corresponding receptor, the protein tyrosine kinase-7 (PTK7) embedded in the membrane of acute lymphoblastic leukemia (ALL) cells (Jurkat T-cells). Performing single molecule force spectroscopy (SMFS) experiments, we showed that the aptamer sgc8c bound with high probability (38.3 ± 7.48%) and high specificity to PTK7, as demonstrated by receptor blocking experiments and through comparison with the binding behavior of a nonspecific aptamer...
April 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28207294/validity-of-core-temperature-measurements-at-3-rectal-depths-during-rest-exercise-cold-water-immersion-and-recovery
#5
Kevin C Miller, Lexie E Hughes, Blaine C Long, William M Adams, Douglas J Casa
CONTEXT:   No evidence-based recommendation exists regarding how far clinicians should insert a rectal thermistor to obtain the most valid estimate of core temperature. Knowing the validity of temperatures at different rectal depths has implications for exertional heat-stroke (EHS) management. OBJECTIVE:   To determine whether rectal temperature (Trec) taken at 4 cm, 10 cm, or 15 cm from the anal sphincter provides the most valid estimate of core temperature (as determined by esophageal temperature [Teso]) during similar stressors an athlete with EHS may experience...
April 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/28093005/cardiac-and-respiratory-effects-of-deep-regional-hyperthermia-using-an-8-mhz-radiofrequency-capacitive-device-on-patients-with-cancer
#6
Rieko Izukura, Hajime Imada, Nobuko Hashiguchi, Hiroyuki Sawatari, Takayuki Ohguri, Mami Miyazono, Shin Ohta, Chizuko Takakura, Keiko Yamasaki, Chie Magota, Kanae Fujita, Hiromi Kuroda, Hideki Hirata, Tomoko Ohkusa, Akiko Chishaki
PURPOSE: Hyperthermia (HT), an adjuvant therapy for variable cancers, may cause physiological changes in the patients, which may lead to cardiovascular problems. Among various HT treatments, the physiological effects of deep regional HT are still unclear. We examined the physiological alterations throughout deep regional HT to improve the HT safety. MATERIALS AND METHODS: Thirty-one patients (age: 61±12 years) with cancer received HT in the thoracic or upper abdominal regions using an 8-MHz radiofrequency-capacitive-device for 50 min...
January 16, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28069045/improving-nursing-home-care-through-feedback-on-performance-data-inform-protocol-for-a-cluster-randomized-trial
#7
Matthias Hoben, Peter G Norton, Liane R Ginsburg, Ruth A Anderson, Greta G Cummings, Holly J Lanham, Janet E Squires, Deanne Taylor, Adrian S Wagg, Carole A Estabrooks
BACKGROUND: Audit and feedback is effective in improving the quality of care. However, methods and results of international studies are heterogeneous, and studies have been criticized for a lack of systematic use of theory. In TREC (Translating Research in Elder Care), a longitudinal health services research program, we collect comprehensive data from care providers and residents in Canadian nursing homes to improve quality of care and life of residents, and quality of worklife of caregivers...
January 10, 2017: Trials
https://www.readbyqxmd.com/read/28065337/profound-t-cell-lymphopenia-associated-with-prenatal-exposure-to-purine-antagonists-detected-by-trec-newborn-screening
#8
Caroline Y Kuo, Maria I Garcia-Lloret, Patricia Slev, John F Bohnsack, Karin Chen
No abstract text is available yet for this article.
January 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28056841/thymus-activity-measured-by-t-cell-receptor-excision-circles-in-patients-with-different-severities-of-respiratory-syncytial-virus-infection
#9
Kiran Aftab Gul, Tonje Sonerud, Hans O Fjærli, Britt Nakstad, Tore Gunnar Abrahamsen, Christopher S Inchley
BACKGROUND: Respiratory syncytial virus (RSV) infection is an important cause of hospitalization in previously healthy infants. Immunological mechanisms predisposing infants to severe disease are poorly understood. Early biomarkers for disease severity may assist clinical decisions. We investigated T-cell receptor excision circles (TREC), episomal DNA made during thymic T-cell receptor rearrangement, and a marker for thymus activity, both during disease and in neonatal screening cards as a risk factor for RSV disease severity...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28010810/conventional-and-novel-body-temperature-measurement-during-rest-and-exercise-induced-hyperthermia
#10
COMPARATIVE STUDY
Colin Towey, Chris Easton, Robert Simpson, Charles Pedlar
Despite technological advances in thermal sensory equipment, few core temperature (TCORE) measurement techniques have met the established validity criteria in exercise science. Additionally, there is debate as to what method serves as the most practically viable, yet upholds the proposed measurement accuracy. This study assessed the accuracy of current and novel TCORE measurement techniques in comparison to rectal temperature (TREC) as a reference standard. Fifteen well-trained subjects (11 male, 4 female) completed 60min of exercise at an intensity equating to the lactate threshold; measured via a discontinuous exercise test...
January 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/27998477/advanced-portrayal-of-smil-coating-by-allying-cze-performance-with-in-capillary-topographic-and-charge-related-surface-characterization
#11
Lorenz G Stock, Michael Leitner, Lukas Traxler, Klaus Bonazza, Laurent Leclercq, Hervé Cottet, Gernot Friedbacher, Andreas Ebner, Hanno Stutz
A successive multiple ionic polymer layer (SMIL) coating composed of four layers improved the capillary electrophoretic separation of a recombinant major birch pollen allergen and closely related variants when poly(acrylamide-co-2-acrylamido-2-methyl-1-propansulfonate) (55% PAMAMPS) replaced dextran sulfate as terminal SMIL layer. 55% PAMAMPS decelerated the electroosmotic flow (EOF) due to its lower charge density. Atomic force microscopy (AFM) was used to investigate SMIL properties directly on the inner capillary surface and to relate them to EOF measurements and results of associated CZE separations of a mixture of model proteins and peptides that were performed in the same capillary...
January 25, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27974734/antibiofilm-and-anti-%C3%AE-lactamase-activities-of-burdock-root-extract-and-chlorogenic-acid-against-klebsiella-pneumoniae
#12
Satish Kumar Rajasekharan, Samiraj Ramesh, Ann Susan Satish, Jintae Lee
Small phytochemicals have been successfully adopted as antibacterial chemotherapies and are being increasingly viewed as potential antibiofilm agents. Some of these molecules are known to repress biofilm and toxin production by certain bacterial and yeast pathogens, but information is lacking with regard to the genes allied with biofilm formation. The present study was performed to investigate the inhibitory effect of burdock root extract (BRE) and of chlorogenic acid (CGA; a component of BRE) on clinical isolates of Klebsiella pneumoniae...
March 28, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27916705/newborn-screening-for-severe-combined-immunodeficiency-using-a-novel-and-simplified-method-to-measure-t-cell-excision-circles-trec
#13
Laura Tagliaferri, Joachim B Kunz, Margit Happich, Susanna Esposito, Thomas Bruckner, Daniel Hübschmann, Jürgen G Okun, Georg F Hoffmann, Ansgar Schulz, Judit Kappe, Carsten Speckmann, Martina U Muckenthaler, Andreas E Kulozik
The prognosis of children with severe combined immunodeficiency (SCID) depends on a presymptomatic diagnosis and early treatment before complications occur. We established and tested a simplified, practical and economic newborn screening method based on the quantification of T-cell receptor excision circles (TRECs) on dried blood spots (DBSs) through qPCR. Our method was validated by the analysis of 11 positive controls, which all showed an absence of TRECs, thus yielding a sensitivity of 100%. Further, we analyzed 6034 anonymized newborns of whom 6031 (99,95%) showed a normal TREC qPCR with a median of 600 estimated TREC copies/1...
December 2, 2016: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/27902982/limitation-of-simultaneous-analysis-of-t-cell-receptor-and-%C3%AE%C2%BA-deleting-recombination-excision-circles-based-on-multiplex-real-time-polymerase-chain-reaction-in-common-variable-immunodeficiency-patients
#14
Faranaz Atschekzei, Fareed Ahmad, Torsten Witte, Roland Jacobs, Reinhold E Schmidt
AIM OF STUDY: We used a triplex real-time polymerase chain reaction (PCR) to classify our common variable immunodeficiency (CVID) patients into distinct groups according to the amount of their T-cell receptor excision circles (TRECs) and κ-deleting recombination excision circles (KRECs). MATERIALS AND METHODS: TREC and KREC analysis was performed using a multiplex real-time PCR assay. The T- and B-lymphocyte subsets were measured by flow cytometry. RESULTS: The copy number of TRECs and KRECs was significantly reduced in CVID patients compared to healthy controls...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27880933/immunological-effects-of-nilotinib-prophylaxis-after-allogeneic-stem-cell-transplantation-in-patients-with-advanced-chronic-myeloid-leukemia-or-philadelphia-chromosome-positive-acute-lymphoblastic-leukemia
#15
Nira Varda-Bloom, Ivetta Danylesko, Roni Shouval, Shiran Eldror, Atar Lev, Jacqueline Davidson, Esther Rosenthal, Yulia Volchek, Noga Shem-Tov, Ronit Yerushalmi, Avichai Shimoni, Raz Somech, Arnon Nagler
Allogeneic stem cell transplantation remains the standard treatment for resistant advanced chronic myeloid leukemia and Philadelphia chromosome-positive acute lymphoblastic leukemia. Relapse is the major cause of treatment failure in both diseases. Post-allo-SCT administration of TKIs could potentially reduce relapse rates, but concerns regarding their effect on immune reconstitution have been raised. We aimed to assess immune functions of 12 advanced CML and Ph+ ALL patients who received post-allo-SCT nilotinib...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/27873105/newborn-screening-for-severe-primary-immunodeficiency-diseases-in-sweden-a-2-year-pilot-trec-and-krec-screening-study
#16
Michela Barbaro, Annika Ohlsson, Stephan Borte, Susanne Jonsson, Rolf H Zetterström, Jovanka King, Jacek Winiarski, Ulrika von Döbeln, Lennart Hammarström
Newborn screening for severe primary immunodeficiencies (PID), characterized by T and/or B cell lymphopenia, was carried out in a pilot program in the Stockholm County, Sweden, over a 2-year period, encompassing 58,834 children. T cell receptor excision circles (TREC) and kappa-deleting recombination excision circles (KREC) were measured simultaneously using a quantitative PCR-based method on DNA extracted from dried blood spots (DBS), with beta-actin serving as a quality control for DNA quantity. Diagnostic cutoff levels enabling identification of newborns with milder and reversible T and/or B cell lymphopenia were also evaluated...
January 2017: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/27864993/thyrotropin-alters-t-cell-development-in-the-thymus-in-subclinical-hypothyroidism-mouse-model
#17
K Wu, M Zhao, C Ma, H Zhang, X Liu, L Zhou, J Zhao, L Gao, D Wang
Subclinical hypothyroidism (SCH) is highly prevalent in the general population and is associated with potential deleterious effects. Although developing T cells express thyroid-stimulating hormone receptor (TSH-R), the changes of T cell development in thymus in SCH have not been fully clarified. SCH mouse model, which is characterized by elevated serum TSH but similar thyroid hormone levels, was used to study the role of TSH in T cell development. Thymus weight of SCH mice increased 18% compared with controls...
January 2017: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/27857951/validation-of-an-ingestible-temperature-data-logging-and-telemetry-system-during-exercise-in-the-heat
#18
Gavin J S Travers, David S Nichols, Abdulaziz Farooq, Sébastien Racinais, Julien D Périard
Aim: Intestinal temperature telemetry systems are promising monitoring and research tools in athletes. However, the additional equipment that must be carried to continuously record temperature data limits their use to training. The purpose of this study was to assess the validity and reliability of a new gastrointestinal temperature data logging and telemetry system (e-Celsius™) during water bath experimentation and exercise trials. Materials and Methods: Temperature readings of 23 pairs of e-Celsius (TeC) and VitalSense (TVS) ingestible capsules were compared to rectal thermistor responses (Trec) at 35, 38...
April 2016: Temperature: Multidisciplinary Biomedical Journal
https://www.readbyqxmd.com/read/27824893/an-age-associated-decline-in-thymic-output-differs-in-dog-breeds-according-to-their-longevity
#19
Angela Holder, Stephanie Mella, Donald B Palmer, Richard Aspinall, Brian Catchpole
The age associated decline in immune function is preceded in mammals by a reduction in thymic output. Furthermore, there is increasing evidence of a link between immune competence and lifespan. One approach to determining thymic output is to quantify signal joint T cell receptor excision circles (sj-TRECs), a method which has been developed and used in several mammalian species. Life expectancy and the rate of aging vary in dogs depending upon their breed. In this study, we quantified sj-TRECs in blood samples from dogs of selected breeds to determine whether there was a relationship between longevity and thymic output...
2016: PloS One
https://www.readbyqxmd.com/read/27789773/effect-of-hypohydration-on-thermoregulatory-responses-in-males-with-low-and-high-body-fat-exercising-in-the-heat
#20
Matthew A Tucker, Aaron R Caldwell, Cory L Butts, Forrest B Robinson, Haley C Reynebeau, Stavros A Kavouras, Brendon P McDermott, Tyrone Anthony Washington, Ronna C Turner, Matthew S Ganio
It is unclear if males with low body fat (LO-BF) have impaired thermoregulation during exercise heat stress compared to those with high body fat (HI-BF) when euhydration (EU) is maintained. Further, in LO-BF individuals, hypohydration (HY) impairs thermoregulatory responses during exercise heat stress, but it is unknown if this occurs in HI-BF counterparts. PURPOSE: To test the hypotheses that males with HI-BF have impaired thermoregulatory responses to exercise heat-stress, and HY further exacerbates these impairments versus LO-BF...
October 27, 2016: Journal of Applied Physiology
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