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https://www.readbyqxmd.com/read/27902982/limitation-of-simultaneous-analysis-of-t-cell-receptor-and-%C3%AE%C2%BA-deleting-recombination-excision-circles-based-on-multiplex-real-time-polymerase-chain-reaction-in-common-variable-immunodeficiency-patients
#1
Faranaz Atschekzei, Fareed Ahmad, Torsten Witte, Roland Jacobs, Reinhold E Schmidt
AIM OF STUDY: We used a triplex real-time polymerase chain reaction (PCR) to classify our common variable immunodeficiency (CVID) patients into distinct groups according to the amount of their T-cell receptor excision circles (TRECs) and κ-deleting recombination excision circles (KRECs). MATERIALS AND METHODS: TREC and KREC analysis was performed using a multiplex real-time PCR assay. The T- and B-lymphocyte subsets were measured by flow cytometry. RESULTS: The copy number of TRECs and KRECs was significantly reduced in CVID patients compared to healthy controls...
December 1, 2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27880933/immunological-effects-of-nilotinib-prophylaxis-after-allogeneic-stem-cell-transplantation-in-patients-with-advanced-chronic-myeloid-leukemia-or-philadelphia-chromosome-positive-acute-lymphoblastic-leukemia
#2
Nira Varda-Bloom, Ivetta Danylesko, Roni Shouval, Shiran Eldror, Atar Lev, Jacqueline Davidson, Esther Rosenthal, Yulia Volchek, Noga Shem-Tov, Ronit Yerushalmi, Avichai Shimoni, Raz Somech, Arnon Nagler
Allogeneic stem cell transplantation remains the standard treatment for resistant advanced chronic myeloid leukemia and Philadelphia chromosome-positive acute lymphoblastic leukemia. Relapse is the major cause of treatment failure in both diseases. Post-allo-SCT administration of TKIs could potentially reduce relapse rates, but concerns regarding their effect on immune reconstitution have been raised. We aimed to assess immune functions of 12 advanced CML and Ph+ ALL patients who received post-allo-SCT nilotinib...
November 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27873105/newborn-screening-for-severe-primary-immunodeficiency-diseases-in-sweden-a-2-year-pilot-trec-and-krec-screening-study
#3
Michela Barbaro, Annika Ohlsson, Stephan Borte, Susanne Jonsson, Rolf H Zetterström, Jovanka King, Jacek Winiarski, Ulrika von Döbeln, Lennart Hammarström
Newborn screening for severe primary immunodeficiencies (PID), characterized by T and/or B cell lymphopenia, was carried out in a pilot program in the Stockholm County, Sweden, over a 2-year period, encompassing 58,834 children. T cell receptor excision circles (TREC) and kappa-deleting recombination excision circles (KREC) were measured simultaneously using a quantitative PCR-based method on DNA extracted from dried blood spots (DBS), with beta-actin serving as a quality control for DNA quantity. Diagnostic cutoff levels enabling identification of newborns with milder and reversible T and/or B cell lymphopenia were also evaluated...
November 21, 2016: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/27864993/thyrotropin-alters-t-cell-development-in-the-thymus-in-subclinical-hypothyroidism-mouse-model
#4
Kunpeng Wu, Meng Zhao, Chunyan Ma, Haiqing Zhang, Xiaojing Liu, Lingyan Zhou, Jiajun Zhao, Ling Gao, Dan Wang
Subclinical hypothyroidism (SCH) is highly prevalent in the general population and is associated with potential deleterious effects. Although developing T cells express thyroid stimulating hormone receptor (TSH-R), the changes of T cell development in thymus in SCH have not been fully clarified. SCH mouse model, which is characterized by elevated serum TSH but similar thyroid hormone levels, were used to study the role of TSH in T cell development. Thymus weight of SCH mice increased 18% compared with controls...
November 16, 2016: Scandinavian Journal of Immunology
https://www.readbyqxmd.com/read/27857951/validation-of-an-ingestible-temperature-data-logging-and-telemetry-system-during-exercise-in-the-heat
#5
Gavin J S Travers, David S Nichols, Abdulaziz Farooq, Sébastien Racinais, Julien D Périard
Aim: Intestinal temperature telemetry systems are promising monitoring and research tools in athletes. However, the additional equipment that must be carried to continuously record temperature data limits their use to training. The purpose of this study was to assess the validity and reliability of a new gastrointestinal temperature data logging and telemetry system (e-Celsius™) during water bath experimentation and exercise trials. Materials and Methods: Temperature readings of 23 pairs of e-Celsius (TeC) and VitalSense (TVS) ingestible capsules were compared to rectal thermistor responses (Trec) at 35, 38...
April 2016: Temperature: Multidisciplinary Biomedical Journal
https://www.readbyqxmd.com/read/27824893/an-age-associated-decline-in-thymic-output-differs-in-dog-breeds-according-to-their-longevity
#6
Angela Holder, Stephanie Mella, Donald B Palmer, Richard Aspinall, Brian Catchpole
The age associated decline in immune function is preceded in mammals by a reduction in thymic output. Furthermore, there is increasing evidence of a link between immune competence and lifespan. One approach to determining thymic output is to quantify signal joint T cell receptor excision circles (sj-TRECs), a method which has been developed and used in several mammalian species. Life expectancy and the rate of aging vary in dogs depending upon their breed. In this study, we quantified sj-TRECs in blood samples from dogs of selected breeds to determine whether there was a relationship between longevity and thymic output...
2016: PloS One
https://www.readbyqxmd.com/read/27789773/effect-of-hypohydration-on-thermoregulatory-responses-in-males-with-low-and-high-body-fat-exercising-in-the-heat
#7
Matthew A Tucker, Aaron R Caldwell, Cory L Butts, Forrest B Robinson, Haley C Reynebeau, Stavros A Kavouras, Brendon P McDermott, Tyrone Anthony Washington, Ronna C Turner, Matthew S Ganio
: It is unclear if males with low body fat (LO-BF) have impaired thermoregulation during exercise heat stress compared to those with high body fat (HI-BF) when euhydration (EU) is maintained. Further, in LO-BF individuals, hypohydration (HY) impairs thermoregulatory responses during exercise heat stress, but it is unknown if this occurs in HI-BF counterparts. PURPOSE: To test the hypotheses that males with HI-BF have impaired thermoregulatory responses to exercise heat-stress, and HY further exacerbates these impairments versus LO-BF...
October 27, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27777141/angiogenic-factors-correlate-with-t-cell-immune-reconstitution-and-clinical-outcomes-after-double-unit-umbilical-cord-blood-transplantation-in-adults
#8
Ioannis Politikos, Haesook Kim, Theodoros Karantanos, Julia Brown, Sean McDonough, Lequn Li, Corey Cutler, Joseph H Antin, Karen K Ballen, Jerome Ritz, Vassiliki A Boussiotis
Umbilical cord blood (UCB) is a valuable graft source for allogeneic hematopoietic stem cell transplantation (HSCT) in patients who lack adult donors. UCB transplantation (UCBT) in adults results in delayed immune reconstitution, leading to high infection-related morbidity and mortality. Angiogenic factors and markers of endothelial dysfunction have biologic and prognostic significance in conventional HSCT, but their role in UCBT has not been investigated. Furthermore, the interplay between angiogenesis and immune reconstitution has not been studied...
October 21, 2016: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/27746351/kinetic-behaviour-of-recombinant-fusarium-solani-lipases-using-monomolecular-films-effect-of-the-heterologous-expression
#9
Raida Jallouli, Madiha Bouali, Youssef Gargouri, Sofiane Bezzine
Two lipases from Fusarium solani, FSL and FSL2, were efficiently expressed in Pichia pastoris. To check the influence of the expression on interfacial properties of FSL and to study kinetic properties of FSL2, interfacial parameters of FSL2, native FSL, untagged recombinant and tagged recombinant forms of FSL were compared using the monomolecular film technique. Kinetic study on the dependence of the stereoselectivity of these lipases on the surface pressure was performed using three dicaprin isomers spread in the form of monomolecular films at the air-water interface...
October 13, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27727179/t-cell-receptor-excision-circle-trec-monitoring-after-allogeneic-stem-cell-transplantation-a-predictive-marker-for-complications-and-clinical-outcome
#10
Ahmed Gaballa, Mikael Sundin, Arwen Stikvoort, Muhamed Abumaree, Mehmet Uzunel, Darius Sairafi, Michael Uhlin
Allogeneic hematopoietic stem cell transplantation (HSCT) is a well-established treatment modality for a variety of malignant diseases as well as for inborn errors of the metabolism or immune system. Regardless of disease origin, good clinical effects are dependent on proper immune reconstitution. T cells are responsible for both the beneficial graft-versus-leukemia (GVL) effect against malignant cells and protection against infections. The immune recovery of T cells relies initially on peripheral expansion of mature cells from the graft and later on the differentiation and maturation from donor-derived hematopoietic stem cells...
October 11, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27699228/decreases-in-thymopoiesis-of-astronauts-returning-from-space-flight
#11
Cara L Benjamin, Raymond P Stowe, Lisa St John, Clarence F Sams, Satish K Mehta, Brian E Crucian, Duane L Pierson, Krishna V Komanduri
Following the advent of molecular assays that measure T cell receptor excision circles (TRECs) present in recent thymic emigrants, it has been conclusively shown that thymopoiesis persists in most adults, but that functional output decreases with age, influencing the maintenance of a diverse and functional T cell receptor (TCR) repertoire. Space flight has been shown to result in a variety of phenotypic and functional changes in human T cells and in the reactivation of latent viruses. While space flight has been shown to influence thymic architecture in rodents, thymopoiesis has not previously been assessed in astronauts...
August 4, 2016: JCI Insight
https://www.readbyqxmd.com/read/27686709/improving-the-text-classification-using-clustering-and-a-novel-hmm-to-reduce-the-dimensionality
#12
A Seara Vieira, L Borrajo, E L Iglesias
In text classification problems, the representation of a document has a strong impact on the performance of learning systems. The high dimensionality of the classical structured representations can lead to burdensome computations due to the great size of real-world data. Consequently, there is a need for reducing the quantity of handled information to improve the classification process. In this paper, we propose a method to reduce the dimensionality of a classical text representation based on a clustering technique to group documents, and a previously developed Hidden Markov Model to represent them...
November 2016: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/27632869/can-temperate-water-immersion-effectively-reduce-rectal-temperature-in-exertional-heat-stroke-situations-a-critically-appraised-topic
#13
Tyler T Truxton, Kevin C Miller
Clinical Scenario Exertional heat stroke (EHS) is a medical emergency which, if left untreated, can result in death. The standard of care for EHS patients includes confirmation of hyperthermia via rectal temperature (Trec) and then immediate cold-water immersion (CWI). While CWI is the fastest way to reduce Trec,(1,2) it may be difficult to lower and maintain water bath temperature in the recommended ranges (1.7°C-15°C [35°F-59°F])(1) because of limited access to ice and/or the bath being exposed to high ambient temperatures for long periods of time...
August 24, 2016: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/27627852/severe-neonatal-cmv-infection-complicated-with-thrombotic-microangiopathy-successfully-treated-with-ganciclovir
#14
Noriko Mitsuiki, Keita Tamanuki, Kenshi Sei, Jun Ito, Aiko Kishi, Kenji Kobayashi, Yoshiho Hatai, Masayuki Nagasawa
We report a neonate of severe cytomegalovirus (CMV) infection who presented vomiting, severe thrombocytopenia and thrombotic microangiopathy (TMA). He showed occasional vomiting at 3 weeks of age and visited us with systemic petechiae at 29 days old. Platelet was markedly decreased to 18,000/μL and fragmented red blood cells were increased in the peripheral blood. Intravenous ganciclovir (GCV) administration was started at 35 days old after detection of CMV in the peripheral blood. His normal values of T-cell receptor excision circles (TREC) and signal joint kappa-deleting recombination excision circles (sjKREC) excluded the possibility of severe immunodeficiency...
September 11, 2016: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/27593166/a-part-of-speech-term-weighting-scheme-for-biomedical-information-retrieval
#15
Yanshan Wang, Stephen Wu, Dingcheng Li, Saeed Mehrabi, Hongfang Liu
In the era of digitalization, information retrieval (IR), which retrieves and ranks documents from large collections according to users' search queries, has been popularly applied in the biomedical domain. Building patient cohorts using electronic health records (EHRs) and searching literature for topics of interest are some IR use cases. Meanwhile, natural language processing (NLP), such as tokenization or Part-Of-Speech (POS) tagging, has been developed for processing clinical documents or biomedical literature...
September 1, 2016: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/27570676/finding-related-publications-extending-the-set-of-terms-used-to-assess-article-similarity
#16
Wei Wei, Rebecca Marmor, Siddharth Singh, Shuang Wang, Dina Demner-Fushman, Tsung-Ting Kuo, Chun-Nan Hsu, Lucila Ohno-Machado
Recommendation of related articles is an important feature of the PubMed. The PubMed Related Citations (PRC) algorithm is the engine that enables this feature, and it leverages information on 22 million citations. We analyzed the performance of the PRC algorithm on 4584 annotated articles from the 2005 Text REtrieval Conference (TREC) Genomics Track data. Our analysis indicated that the PRC highest weighted term was not always consistent with the critical term that was most directly related to the topic of the article...
2016: AMIA Summits on Translational Science Proceedings
https://www.readbyqxmd.com/read/27511024/hsp72-and-hsp90%C3%AE-mrna-transcription-is-characterised-by-large-sustained-changes-in-core-temperature-during-heat-acclimation
#17
Oliver R Gibson, James A Tuttle, Peter W Watt, Neil S Maxwell, Lee Taylor
Increased intracellular heat shock protein-72 (Hsp72) and heat shock protein-90α (Hsp90α) have been implicated as important components of acquired thermotolerance, providing cytoprotection during stress. This experiment determined the physiological responses characterising increases in Hsp72 and Hsp90α mRNA on the first and tenth day of 90-min heat acclimation (in 40.2 °C, 41.0 % relative humidity (RH)) or equivalent normothermic training (in 20 °C, 29 % RH). Pearson's product-moment correlation and stepwise multiple regression were performed to determine relationships between physiological [e...
November 2016: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/27453982/integrating-unified-medical-language-system-and-association-mining-techniques-into-relevance-feedback-for-biomedical-literature-search
#18
Yanqing Ji, Hao Ying, John Tran, Peter Dews, R Michael Massanari
BACKGROUND: Finding highly relevant articles from biomedical databases is challenging not only because it is often difficult to accurately express a user's underlying intention through keywords but also because a keyword-based query normally returns a long list of hits with many citations being unwanted by the user. This paper proposes a novel biomedical literature search system, called BiomedSearch, which supports complex queries and relevance feedback. METHODS: The system employed association mining techniques to build a k-profile representing a user's relevance feedback...
2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/27403355/abnormal-trec-based-newborn-screening-test-in-a-premature-neonate-with-massive-perivillous-fibrin-deposition-of-the-placenta
#19
Stefan Kostadinov, Karen A Robbins, Anthony Hayward
Severe combined immunodeficiency (SCID), a primary immunodeficiency arising from variable defects in lymphocyte development and survival, is characterized by significant deficiency of thymus derived (T-) lymphocytes and variable defects in the B-lymphocyte population. Newborn screening for SCID is based on detection of low numbers of T-cell receptor excision circles (TRECs) by real time quantitative PCR (RT-qPCR). This screening allows for early identification of individuals with SCID and other disorders characterized by T-lymphopenia...
2016: Case Reports in Immunology
https://www.readbyqxmd.com/read/27367027/inflammatory-related-gene-ikk%C3%AE-ikk%C3%AE-ikk%C3%AE-cooperates-to-determine-liver-cancer-stem-cells-progression-by-altering-telomere-via-heterochromatin-protein-1-hotair-axis
#20
Jiahui An, Mengying Wu, Xiaoru Xin, Zhuojia Lin, Xiaonan Li, Qidi Zheng, Xin Gui, Tianming Li, Hu Pu, Haiyan Li, Dongdong Lu
Cancer stem cells are associated with tumor recurrence. IKK is a protein kinase that is composed of IKKα, IKKβ, IKKγ. Herein, we demonstrate that IKKα plus IKKβ promoted and IKKγ inhibited liver cancer stem cell growth in vitro and in vivo. Mechanistically, IKKα plus IKKβ enhanced and IKKγ inhibited the interplay among HP1α, HP1β and HP1γ that competes for the interaction among HP1α, SUZ12, HEZ2. Therefore, IKKα plus IKKβ inhibited and IKKγ enhanced the activity of H3K27 methyltransferase SUZ12 and EZH2, which methylates H3K27 immediately sites on HOTAIR promoter region...
June 29, 2016: Oncotarget
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