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https://www.readbyqxmd.com/read/28927171/evolving-quality-standards-for-large-scale-registries-the-garfield-af-experience
#1
Keith A A Fox, Bernard J Gersh, Sory Traore, A John Camm, Gloria Kayani, Anders Krogh, Shweta Shweta, Ajay K Kakkar
Aims: Registries have the potential to capture treatment practices and outcomes in populations beyond the constraints of clinical trial settings. The value of data obtained depend critically upon robust quality standards (including source data verification [SDV] and training); features that are commonly absent from registries. This article outlines the quality standards developed for Global Anticoagulant Registry in the FIELD-Atrial Fibrillation (GARFIELD-AF). Methods and Results: GARFIELD-AF comprises ∼57 000 patients prospectively recruited over 6...
April 1, 2017: European Heart Journal. Quality of Care & Clinical Outcomes
https://www.readbyqxmd.com/read/28926960/genetic-and-hormonal-regulation-of-chlorophyll-degradation-during-maturation-of-seeds-with-green-embryos
#2
REVIEW
Galina Smolikova, Elena Dolgikh, Maria Vikhnina, Andrej Frolov, Sergei Medvedev
The embryos of some angiosperms (usually referred to as chloroembryos) contain chlorophylls during the whole period of embryogenesis. Developing embryos have photochemically active chloroplasts and are able to produce assimilates, further converted in reserve biopolymers, whereas at the late steps of embryogenesis, seeds undergo dehydration, degradation of chlorophylls, transformation of chloroplast in storage plastids, and enter the dormancy period. However, in some seeds, the process of chlorophyll degradation remains incomplete...
September 16, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28926954/evidence-for-complex-formation-of-the-bacillus-cereus-haemolysin-bl-components-in-solution
#3
Franziska Tausch, Richard Dietrich, Kristina Schauer, Robert Janowski, Dierk Niessing, Erwin Märtlbauer, Nadja Jessberger
Haemolysin BL is an important virulence factor regarding the diarrheal type of food poisoning caused by Bacillus cereus. However, the pathogenic importance of this three-component enterotoxin is difficult to access, as nearly all natural B. cereus culture supernatants additionally contain the highly cytotoxic Nhe, the second three-component toxin involved in the aetiology of B. cereus-induced food-borne diseases. To better address the toxic properties of the Hbl complex, a system for overexpression and purification of functional, cytotoxic, recombinant (r)Hbl components L₂, L₁ and B from E...
September 16, 2017: Toxins
https://www.readbyqxmd.com/read/28926874/-multicenter-epidemiological-investigation-of-hospitalized-elderly-young-and-middle-aged-patients-with-severe-burn
#4
Y Tang, L X Wang, W G Xie, Z A Shen, G H Guo, J J Chen, C M Han, L C Ren, Z G Chu, M F Yin, Y Wang, D X Zhang, Y S Huang, J P Zhang
Objective: To compare and analyze the epidemiological characteristics of hospitalized elderly, young and middle-aged patients with severe burn in recent years, so as to provide reference for the prevention and treatment of elderly patients with severe burn. Methods: Relying on the entry system of epidemiological case data and biological sample of severe burn from multicenter in clinic, medical records of patients with severe burn, aged above 18, hospitalized in 8 burn wards from January 2012 to December 2015 were collected...
September 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28926823/preparation-characterization-and-application-of-urease-nanoparticles-for-construction-of-an-improved-potentiometric-urea-biosensor
#5
Seema Jakhar, C S Pundir
The nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine dihydrochloride. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM), UV and Fourier transform infrared (FTIR) spectroscopy. The TEM images of urease NPs showed their size in the range, 18-100nm with an average of 51.2nm. The ENPs were more active and stable with a longer shelf life than native enzyme molecules...
September 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28926769/experimental-study-of-time-response-of-bending-deformation-of-bone-cantilevers-in-an-electric-field
#6
Huimin Kang, Zhende Hou, Qing-Hua Qin
Bone is a complex composite material with hierarchical structures and anisotropic mechanical properties. Bone also processes electromechanical properties, such as piezoelectricity and streaming potentials, which termed as stress generated potentials. Furthermore, the electrostrictive effect and flexoelectric effect can also affect electromechanical properties of the bone. In the present work, time responses of bending deflections of bone cantilever in an external electric field are measured experimentally to investigate bone's electromechanical behavior...
September 13, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28926733/epidemiology-of-type-1-diabetes-mellitus-in-basrah-southern-iraq-a-retrospective-study
#7
Dhaighum Almahfoodh, Majid Alabbood, Ahmed Alali, Abbas Mansour
AIMS: To investigate the epidemiology of type 1 diabetes mellitus (T1DM) in Basrah city, Southern Iraq, between 2012 and 2016 among people 0-40year old. METHODS: This was a retrospective data analysis of electronic archives for patients with T1DM registered in Faiha Specialized Diabetes, Endocrine, and Metabolism Center (FDEMC), which is a tertiary referring Center in Basrah. The data include electronic database from August 2008 to February 2016. Incidence and prevalence rates are expressed per 100,000...
September 5, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28926704/chromatographic-properties-of-minimal-aspect-ratio-monolithic-silica-columns
#8
Takeshi Hara, Shunta Futagami, Wim De Malsche, Sebastiaan Eeltink, Herman Terryn, Gino V Baron, Gert Desmet
We report on a study wherein we synthesized TMOS-based silica monolithic skeletons in capillaries with an i.d. of 5 and 10 μm to produce skeleton structures with very low capillary-to-domain size aspect-ratios. These structures include the absolute minimal aspect-ratio case of a monolithic structure whose cross-section only contains a single node point. With domain-sized based reduced plate heights running as low as hmin = 1.3-1.5 for retained coumarin dyes providing a retention factor of k = 0.6-1.0, the study confirms the classic observation that ultra-low aspect ratio columns generate a markedly lower dispersion than columns with a larger aspect ratio made in the past by Knox, Jorgenson and Kennedy for the packed bed of spheres, but now for silica monoliths...
September 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28926460/peripheral-bacterial-septic-arthritis-review-of-diagnosis-and-management
#9
Ahmed S Hassan, Allison Rao, Augustine M Manadan, Joel A Block
Septic arthritis refers to an infection in a joint due to a bacterial, mycobacterial, or fungal cause. Joint infections are a serious cause of morbidity and mortality and constitute a true musculoskeletal emergency. The estimated incidence of septic arthritis in the general population is between 2 and 6 cases per 100,000 people per year. The most common presentation is an acute monoarthritis. Identification of organisms in the synovial fluid is the criterion standard for diagnosis. Synovial fluid aspiration should be performed prior to initiating antibiotics...
September 19, 2017: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
https://www.readbyqxmd.com/read/28926140/biological-variation-of-serum-neuron-specific-enolase-and-carbohydrate-antigen-724-tumor-markers
#10
Shuo Wang, Min Zhao, Runqing Mu, Xin Zhang, Ke Yun, Hong Shang
BACKGROUND: The aim of our study was to assess two tumor markers, carbohydrate antigen 724 (CA724) and neuron-specific enolase (NSE), in 20 healthy individuals. Then, we determined their average intra- and inter-individual biological variations (CVI and CVG ). METHODS: Blood samples were taken at weekly intervals for five weeks from 20 healthy subjects through a detailed questionnaire and physical examination. These samples were stored at -80°C and analyzed in a single run in duplicate...
September 19, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28926121/new-pediatric-percentiles-of-liver-enzyme-serum-levels-alt-ast-ggt-effects-of-age-sex-bmi-and-pubertal-stage
#11
Sarah Bussler, Mandy Vogel, Diana Pietzner, Kristian Harms, Theresa Buzek, Melanie Penke, Norman Händel, Antje Körner, Ulrich Baumann, Wieland Kiess, Gunter Flemming
The present study aims to clarify the effects of sex, age, BMI and puberty on transaminase serum levels in children and adolescents and to provide new age- and sex-related percentiles for alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyltransferase (GGT). Venous blood and anthropometric data were collected from 4,126 cases. Excluded were cases of participants with potential hepatotoxic medication, with evidence of potential illness at the time of blood sampling and non-normal BMI (BMI < 10(th) or > 90(th) )...
September 19, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28926059/br%C3%A3-nsted-acidity-in-deep-eutectic-solvents-and-ionic-liquids
#12
Andrew P Abbott, Sahar S M Alabdullah, Azhar Y M Al-Murshedi, Karl S Ryder
Despite the importance of ionic liquids in a variety of fields, little is understood about the behaviour of protons in these media. The main difficulty arises due to the unknown activity of protons in non-aqueous solvents. This study presents acid dissociation constants for nine organic acids in deep eutectic solvents (DESs) using standard pH indicator solutes. The pKIn value for bromophenol blue was found by titrating the DES with triflic acid. The experimental method was developed to understand the acid-base properties of deep eutectic solvents, and through this study it was found that the organic acids studied were slightly less dissociated in the DES than in water with pKa values between 0...
September 19, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28925739/synthesis-of-piperazine-based-thiazolidinones-as-vegfr2-tyrosine-kinase-inhibitors-inducing-apoptosis
#13
Mostafa Mm El-Miligy, Heba A Abd El Razik, Marwa M Abu-Serie
AIM: VEGFR2 tyrosine kinase is a main target in suppressing cancer growth and metastasis. Materials & methods: Piperazine-based thiazolidinones were synthesized and screened for their anticancer and VEGFR2 tyrosine kinase inhibitory activity. Results: Compounds 11, 13 and 16 displayed potent anticancer activity against HepG-2 with IC50 values 0.03-0.06 μM. They were safe on normal human fibroblasts with selectivity indices 8.09, 11.40 and 4.37, respectively. Also, these compounds showed VEGFR2 tyrosine kinase inhibitory activities more than the reference staurosporine with IC50 values <0...
September 19, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28925380/numerical-simulation-of-vortex-dynamics-in-type-ii-superconductors-in-oscillating-magnetic-field-using-time-dependent-ginzburg-landau-equations
#14
Hasnain Mehdi Jafri, Xingqiao Ma, Congpeng Zhao, Deshan Liang, Houbing Huang, Zhuhong Liu, Long-Qing Chen
Time dependent Ginzburg-Landau equations were solved by finite difference scheme for a superconducting sample in steady and oscillating magnetic fields for three-dimensional geometry. Dynamic behaviour of penetrating and leaving magnetic vortices in superconductor with the oscillating magnetic field was simulated. Carrier concentration density and the average magnetization of the sample were studied as a function of external oscillating magnetic field. Anomalies in carrier concentration at certain magnetic field values were observed and discussed...
September 19, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28925149/-research-on-modeling-method-to-analyze-lonicerae-japonicae-flos-extraction-process-with-online-mems-nir-based-on-two-types-of-error-detection-theory
#15
Chen-Zhao Du, Zhi-Sheng Wu, Na Zhao, Zheng Zhou, Xin-Yuan Shi, Yan-Jiang Qiao
To establish a rapid quantitative analysis method for online monitoring of chlorogenic acid in aqueous solution of Lonicera Japonica Flos extraction by using micro-electromechanical near infrared spectroscopy (MEMS-NIR). High performance liquid chromatography(HPLC) was used as reference method.Kennard-Stone (K-S) algorithm was used to divide sample sets, and partial least square(PLS) regression was adopted to establish the multivariate analysis model between the HPLC analysis contents and NIR spectra. The synergy interval partial least squares (SiPLS) was used to selected modeling waveband to establish PLS models...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925146/-rapid-monitoring-five-components-of-ethanol-precipitation-process-of-shenzhiling-oral-solution-using-near-infrared-spectroscopy
#16
Tong-Tong Li, Tian Hu, Lei Nie, Li-Xuan Zang, Ying-Zi Zeng, Heng-Chang Zang
To develop a method for the rapid monitoring of five components during the alcohol precipitation process of Shenzhiling oral solution using near infrared spectroscopy(NIRS).The contents of five components detemined by high performance liquid chromatography(HPLC) were used as the reference values, and the NIRS based partial least square regression(PLSR) models were used to monitor the concentrations of paeoniflorin, albiflorin, liquiritin, cinnamic acid and glycyrrhizic acid during the alcohol precipitation process of Shenzhiling oral solution, which were optimized and verified through comparing of different spectral pre-processing and variables selection methods...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28925005/simultaneous-qualitative-and-quantitative-analyses-of-triterpenoids-in-ilex-pubescens-by-ultra-high-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry
#17
Di Cao, Qing Wang, Jing Jin, Maosong Qiu, Lian Zhou, Xinghong Zhou, Hui Li, Zhongxiang Zhao
INTRODUCTION: Ilex pubescens Hook et Arn mainly contains triterpenoids that possess antithrombotic, anti-inflammatory and analgesic effects. Quantitative and qualitative analyses of the triterpenoids in I. pubescens can be useful for determining the authenticity and quality of raw materials and guiding its clinical preparation. OBJECTIVES: To establish a method for rapid and comprehensive analysis of triterpenoids in I. pubescens using ultra-high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS), which will also be applied to evaluate the contents of nine triterpenoids among root, root heartwood and root bark of I...
September 18, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28924601/incidental-colorectal-focal-18-f-fdg-uptake-a-novel-indication-for-colonoscopy
#18
Eugénie Rigault, Laurence Lenoir, Guillaume Bouguen, Mael Pagenault, Astrid Lièvre, Etienne Garin, Laurent Siproudhis, Jean-François Bretagne
BACKGROUND AND STUDY AIMS:  The relevance of incidental colorectal focal (18) F-FDG PET/CT uptake is debatable. All patients who were referred for colonoscopy because of incidental colonic focal FDG uptake were included in this retrospective study. PATIENTS AND METHODS:  PET/CT imaging characteristics were reviewed by a nuclear physician who was blinded to endoscopic and histopathological findings to determine the location of FDG uptake sites and to measure the maximum standardized uptake values (SUVmax) and metabolic volume (MV)...
September 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28924541/the-lrp1-gene-polymorphism-is-associated-with-increased-risk-of-metabolic-syndrome-prevalence-in-the-serbian-population
#19
N Vučinić, E Stokić, I Djan, D Obreht, N Veličković, K Stankov, M Djan
The determination of genetic background in metabolic syndrome (MetS) represents one of the necessary steps to prevent the disorder, thus reducing the cost of medical treatments and helping to design targeted therapy. The study explores the association between individual alleles of the LRP1 gene and the diagnosis of MetS to find correlation between the low-density lipoprotein receptor-related (LRP1) gene polymorphism and each individual anthropometric and biochemical parameter. The study included 93 males and females, aged from 19 to 65, divided into two groups...
June 30, 2017: Balkan Journal of Medical Genetics: BJMG
https://www.readbyqxmd.com/read/28924465/brain-dead-donation-rate-in-month-of-ramadan-and-the-other-months-2005-2014
#20
M Aghighi, M Mahdavi-Mazdeh, M Saberi Isfeedvajani, S A Tavakoli, N Tirgar, A Heidary Rouchi
Regardless of the level of development, religion and beliefs have crucial impact on people's attitude towards organ donation. Although organ donation in Islam is obviously appraised, mainly due to lack of an appropriate infrastructure, post-mortem donation rate in Islamic countries is not comparable to successful settings. We conducted this study to assess the extent of contribution of factors that reduce the level of effectiveness, and also to determine the impact of altruistic feelings in the month of Ramadan on family refusal as the leading modifiable contributor to organ donation rate...
2017: International Journal of Organ Transplantation Medicine
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