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Stella Maris Lins Terena, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori, Aldo Brugnera Junior, Daniela de Fátima Teixeira da Silva, Eric Moreno Ramos Magalhães, Raquel Agnelli Mesquita Ferrari
BACKGROUND: The photobiomodulation using the low-level laser therapy (LLLT) exerts a positive modulating effect on the synthesis of collagen in skeletal muscles and tendons. However, few studies have addressed this effect during the compensatory overload. OBJECTIVE: Evaluate the effect of infrared laser on the deposition and organization of collagen fibers in muscle and tendon tissue during compensatory overload of the plantar muscle in rats. MATERIALS AND METHODS: Wistar rats were submitted to bilateral ablation of the synergist muscles of the hind paws and divided in groups: Control, Hypertrophy, and Hypertrophy (H)+LLLT (780 nm, 40 mW, 9...
February 21, 2018: Photomedicine and Laser Surgery
Chiara Locatelli, Danitza Pradelli, Giulia Campo, Ilaria Spalla, Alice Savarese, Paola G Brambilla, Claudio Bussadori
Objectives Large studies focusing on restrictive cardiomyopathy (RCM) in the cat are scarce. The aims of this retrospective study were to describe epidemiological characteristics and to analyse prognostic factors affecting survival in cats with RCM. Methods The clinical archives of the Gran Sasso Veterinary Clinic (Milan, Italy) and of the cardiology unit of the Department of Veterinary Medicine (University of Milan, Italy) from 1997-2015 were reviewed for all cats diagnosed with RCM based on an echocardiographic examination (left atrial/bi-atrial enlargement, normal left ventricle wall thickness, normal or mildly decreased systolic function and restrictive left ventricle filling pattern with pulsed Doppler echocardiography)...
February 1, 2018: Journal of Feline Medicine and Surgery
Danielle De Lima Rodrigues, Agnelo Neves Alves, Beatriz Ribeiro Guimarães, Weslley Wallace de Alcântara Araujo Amorim, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari
The aim of the present study was to evaluate the effects of LLLT prior to muscle injury with and without post-injury irradiation on the expression of isoforms of myosin heavy chain (MyHC), calcineurin (CaN), and myostatin during the repair process. Wistar rats were divided into five groups: control (n = 7); injury (n = 21); LLLT + injury (n = 21); injury + LLLT (n = 21), and LLLT + injury + LLLT (n = 21). Cryoinjury was performed on the tibialis anterior (TA) muscle. The injured groups were euthanized at 3, 7, and 14 days after injury...
February 15, 2018: Lasers in Medical Science
Fernanda Cordeiro da Silva, Andréa Oliver Gomes, Paulo Roberto da Costa Palácio, Fabiano Politti, Daniela de Fátima Teixeira da Silva, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori
Photobiomodulation is a treatment that has been widely used in neurotrauma and neurodegenerative diseases. In the present study, low-level laser therapy was administered to patients with spinal cord injury. Twenty-five individuals were divided into two groups: placebo photobiomodulation plus physiotherapy and active photobiomodulation plus physiotherapy. Electromyographic evaluations were performed before and after 12 sessions of phototherapy as well as 30 days after the end of treatment. In the active phototherapy group, median frequency values of the brachial biceps and femoral quadriceps muscles were higher at rest and during isotonic contraction 30 days after photobiomodulation (p = 0...
February 13, 2018: Lasers in Medical Science
Camila Basilio Okamoto, Lara Jasinski Motta, Renato Araujo Prates, Ana Carolina Costa da Mota, Marcela Leticia Leal Gonçalves, Anna Carolina Ratto Tempestini Horliana, Raquel Agnelli Mesquita Ferrari, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori
To assure the success of endodontic treatment, the root canal must be completely decontaminated. Thus, some methods have been researched in an attempt to effectively eliminate microorganisms from the interior of the root canal system. Among them, is conventional endodontic therapy in conjunction with photodynamic therapy (PDT). Five anterior deciduous teeth were treated endodontically with the conventional technique and PDT. For PDT, 0.005% methylene blue was used as a photosensitizer and a 660nm laser. After the conventional endodontic therapy, the photosensitizer was applied inside the root canal, 3 minutes of pre-irradiation time and then the laser was applied for 40s with an energy of 4J...
February 8, 2018: Photochemistry and Photobiology
Lucas Andreo, Raquel A Mesquita-Ferrari, Beatriz G Ribeiro, Adriana Benitte, Tatiane de Fátima Nogueira, Cristiane M França, Daniela de Fátima Teixeira da Silva, Sandra K Bussadori, Kristianne P S Fernandes, Fernanda I Corrêa, João C F Corrêa
OBJECTIVE: The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. MATERIALS AND METHODS: Sixty-five Wistar rats were divided into five groups: Control-animals not submitted to any procedure; Injury-cryoinjury of the tibialis anterior muscle; HBSS-animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells-animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT-animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3...
February 5, 2018: Lasers in Surgery and Medicine
Luciana G Langella, Paula F C Silva, Larissa Costa-Santos, Marcela L L Gonçalves, Lara J Motta, Alessandro M Deana, Kristianne P S Fernandes, Raquel A Mesquita-Ferrari, Sandra Kalil Bussadori
BACKGROUND: Temporomandibular disorder (TMD) is described as a subgroup of orofacial pain with a set of signs and symptoms that involve the temporomandibular joint, masticatory muscles, ears, and neck. TMD can occur unilaterally or bilaterally and approximately 70% of the population is affected with at least one sign. The disorder progresses with orofacial pain, muscle pain involving the masticatory and cervical muscles, joint noises (clicks and pops), joint block, mandibular dysfunction, and headache...
January 26, 2018: Trials
Marcela Leticia Leal Gonçalves, Antonio Carlos da Silva Tavares, Ana Carolina Costa da Mota, Luiz Alberto Plácido Penna, Alessandro Melo Deana, Sandra Kalil Bussadori
The aim of the present study was to evaluate colorimetric changes and tooth sensitivity in adolescents and young patients submitted to tooth bleaching with 20% and 35% hydrogen peroxide. A randomized, controlled, clinical trial was conducted with 53 patients aged 11 to 24 years who were allocated to groups based on the use of the following commercial products: Whiteness HP - FGM® (35% hydrogen peroxide); Whiteness HP Blue Calcium - FGM® (35% hydrogen peroxide); and Whiteness HP Blue Calcium - FGM® (20% hydrogen peroxide)...
November 2017: Brazilian Dental Journal
Carolina Carvalho Bortoletto, Mônica da Consolação Canuto Salgueiro, Renata Valio, Yara Dadalti Fragoso, Pamella de Barros Motta, Lara Jansiski Motta, Fernanda Yukie Kobayashi, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Alessandro Deana, Sandra Kalil Bussadori
[Purpose] Present study aimed to evaluate the relationship between sleep bruxism and headache in school children. [Subjects and Methods] This study was conducted with 103 children aged 3-6 years. The exclusion criteria were early tooth loss, dental appliance was used, physical or psychological limitations, chronic disease and continuous medication. Sleep bruxism was diagnosed based on an indication by parents of the occurrence of teeth clenching/grinding and incisor/occlusal tooth wear, following the criteria of the American Academy of Sleep Medicine...
November 2017: Journal of Physical Therapy Science
Evaldo Moreira da Silva Junior, Raquel Agnelli Mesquita-Ferrari, Cristiane Miranda França, Lucas Andreo, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes
AIMS: The purpose of this study was toclarify the effect of low intensity pulsed ultrasound (LIPUS) on the phenotype of the inflammatory infiltrate in muscle tissue following acute injury. MAIN METHODS: Forty Wistar rats were submitted to cryoinjury of the tibialis anterior muscle and only half were treated with LIPUS. After 1, 2, 3 and 7 days macrophages and neutrophils were quantified. KEY FINDINGS: With one day, LIPUS led to reductions in the number of neutrophils and M1 macrophages...
December 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Adriana de Brito, Agnelo Neves Alves, Beatriz Guimaraes Ribeiro, Daniel Victor D Emilio Barbosa, Erick Moreno Ramos Magalhaes, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori, Juliana Barbosa Goulardins, Raquel Agnelli Mesquita-Ferrari
The purpose of this study was to evaluate the effects of low-level laser therapy (LLLT) on morphological aspects, IL-6 and IL-1β expressions, as well as the distribution and organization of collagen in the tibialis anterior (TA) muscle of elderly rats submitted to cryoinjury. Histological photomicrographs were taken of TA muscles stained with HE and picrosirius red. Immunohistochemistry was used for the evaluation of IL-6 and IL-1β. Male Wistar rats, aged 20 months, were distributed into three groups: (1) control animals not injured or treated with LLLT (n = 5), (2) cryoinjury without LLLT treatment (n = 15), and (3) cryoinjury treated with infrared LLLT (n = 15)...
November 27, 2017: Lasers in Medical Science
Mariana Moreira da Silva, Regiane Albertini, Paulo de Tarso Camillo de Carvalho, Ernesto Cesar Pinto Leal-Junior, Sandra Kalil Bussadori, Stella Sousa Vieira, Danilo Sales Bocalini, Luis Vicente Franco de Oliveira, Vanessa Grandinetti, José Antonio Silva, Andrey Jorge Serra
This study evaluated the role of the phototherapy and exercise training (EXT) as well as the combined treatment in general symptoms, pain, and quality of life in women suffering from fibromyalgia (FM). A total of 160 women were enrolled and measures were carried out in two sets: it was sought to identify the acute effect for a single phototherapy and EXT session (Set 1); long-term effect (10 weeks) of the interventions (Set 2). Phototherapy irradiation was performed at 11 locations in their bodies, employing a cluster with nine diodes (one super-pulsed infrared 905 nm, four light-emitting diodes [LEDs] of 640 nm, and four LEDs of 875 nm, 39...
February 2018: Lasers in Medical Science
Larissa Carbonera Candeo, Nicole Cristine Rigonato-Oliveira, Aurileia Aparecida Brito, Rodrigo Labat Marcos, Cristiane Miranda França, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Sandra Kalil Bussadori, Rodolfo Paula Vieira, Adriana Lino-Dos-Santos-Franco, Ana Paula Ligeiro-Oliveira, Anna Carolina Ratto Tempestini Horliana
To evaluate whether periodontitis modulates lung inflammation in an experimental model of asthma as well as the photodynamic therapy (PDT) is associated with a reduction of lung inflammation. Seventy-two BALB/c male mice (~2 months) were randomly divided into 8 groups (n = 9): Basal, Periodontitis (P), P+PT, P+PT+PDT, Asthma (A), A+P, A+P+PT, and A+P+PT+PDT. Periodontitis was induced by using the ligature technique and asthma was induced by ovalbumin (OVA). PT was performed with curettes and PDT with methylene blue (0...
2017: PloS One
L Andreo, C B Soldera, B G Ribeiro, P R V de Matos, S K Bussadori, K P S Fernandes, R A Mesquita-Ferrari
Phototherapy has demonstrated positive effects in the treatment of peripheral nerve injury, but there is a need to investigate the dosimetric parameters. Thus, the aim of the present study was to conduct a literature review on the effects of photobiomodulation with the use of low-level laser therapy (LLLT) on the treatment of peripheral nerve injury in experimental models. The databases of PubMed/MEDLINE, SCOPUS, and SPIE Digital Library were searched for articles on the use of LLLT in experimental models of peripheral nerve injury published in English between January 2007 and March 2016...
December 2017: Lasers in Medical Science
Leandro Lauriti, João Gualberto de Cerqueira Luz, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes, Alessandro Melo Deana, Anna Carolina Ratto Tempestini Horliana, Larissa Costa-Santos, Aldo Brugnera Junior, Sandra Kalil Bussadori
OBJECTIVE: The aim of this study was to evaluate the effect of phototherapy on bite force, facial swelling, mandibular movements, and pain in patients having undergone surgical treatment for mandibular fractures. BACKGROUND: These are among the predominant types of facial fractures, and treatment involving surgical fixation with titanium plates is one of the most common procedures in oral-maxillofacial surgery. Phototherapy has been used to accelerate the muscle healing process and significantly improves muscle regeneration by inducing the formation of new muscle fibers...
October 12, 2017: Photomedicine and Laser Surgery
Sergio Brossi Botta, Patricia Aparecida Ana, Marcela Leticia Leal Gonçalves, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Renato de Araújo Prates, Aldo Brugnera Junior, Sandra Kalil Bussadori
OBJECTIVE: The aim of this in vitro study was to evaluate the degradation of type I collagen fibers after treatment with a papain-based gel associated with a blue dye (PapaMBlue™) for use in antimicrobial photodynamic therapy. MATERIALS AND METHODS: For such, 60 bioabsorbable membrane sponge discs were used. Group 1 was the negative control group. In groups 2, 3, and 4, the papain-based gel PapaMBlue gel was applied all over the samples for 4 min and irradiated using red laser (660 ± 10 nm) with 15, 30, and 40 J/cm(2), respectively...
October 12, 2017: Photomedicine and Laser Surgery
Katia Llanos do Vale, Durvanei Augusto Maria, Lara Cristina Picoli, Alessandro Melo Deana, Marcelo Betti Mascaro, Raquel Agnelli Mesquita Ferrari, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes
OBJECTIVE: Photobiomodulation (PBM) can modulate the proliferation of some types of stem cells. However, few reports have addressed the effects of PBM delivered by light-emitting diode (LED) on stem cells obtained from the pulp tissue of deciduous teeth. The aim of the present study was to investigate the effect of PBM delivered by red LED (630 nm, 75 mW, 37 mW/cm(2)) with different radiant exposures on the cell cycle, mitochondrial membrane potential, and senescence of stem cells from human exfoliated deciduous teeth (SHED)...
September 21, 2017: Photomedicine and Laser Surgery
Mônica da Consolação Canuto Salgueiro, Carolina Carvalho Bortoletto, Anna Carolina RattoTempestini Horliana, Ana Carolina Costa Mota, Lara Jansiski Motta, Pamella de Barros Motta, Raquel Agnelli MesquitaFerrari, Kristianne Porta Santos Fernandes, Sandra Kalil Bussadori
BACKGROUND: Bruxism is a repetitive activity that causes tooth wear, audible sounds, and discomfort. Preventive measures have been studied for conditions that can exert a negative influence on physiological development in children. Low-level laser therapy administered over acupoints is an effective, painless, low-cost treatment option that has achieved good results. Thus, the aim of the proposed study is to evaluate changes in muscle activity, bite force and salivary cortisol in children with bruxism after the application of low-level laser to accupoints...
August 8, 2017: BMC Complementary and Alternative Medicine
Marcela Leticia Leal Gonçalves, Sandra Kalil Bussadori, Yara Dadalti Fragoso, Vinicius Vieira Belarmino da Silva, Alessandro Melo Deana, Ana Carolina Costa da Mota, Erika Horácio Pinto, Anna Carolina RattoTempestini Horliana, Cristiane Miranda França
Smell and odours play a vital role in social interaction. Halitosis is a social problem that affects one third of the population, causing a negative impact on the quality of life. There is little knowledge on the prevalence and management of halitosis in multiple sclerosis (MS) patients. The present study aims to evaluate the presence of halitosis in patients with MS when compared to a control group, and also evaluate treatment of the problem with antimicrobial photodynamic therapy (aPDT). This is a case-control clinical study in which 60 patients were evaluated: 30 MS patients in treatment at the Specialties Clinic School of Medicine, and 30 healthy patients, matched in age and gender for the control group...
October 27, 2017: Journal of Breath Research
Cintia R Lima Leal, Letícia H Alvarenga, Tamires Oliveira-Silva, Ilka T Kato, Bianca Godoy-Miranda, Sandra K Bussadori, Martha S Ribeiro, Renato A Prates
BACKGROUND: Dental caries are a multifactorial disease that progressively produces tooth destruction as a result of bacterial colonization of enamel surface, especially Streptococcus mutans. The objective of this work was to investigate the role of glucose in antimicrobial photodynamic therapy (aPDT) on S. mutans. METHODS: S. mutans ATCC 25175 were cultured on microaerophilia at 37°C for 48h, and we tested aPDT in the presence of 50mM glucose. Bacterial suspension was used to investigate aPDT with 100μM methylene blue (MB) under LED emitting radiation at ʎ=660nm and parameters as following (P=473 mW; I=166...
September 2017: Photodiagnosis and Photodynamic Therapy
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